CN110586220A - Liquid feeding and sample feeding device for micro-fluidic chip - Google Patents
Liquid feeding and sample feeding device for micro-fluidic chip Download PDFInfo
- Publication number
- CN110586220A CN110586220A CN201910982678.7A CN201910982678A CN110586220A CN 110586220 A CN110586220 A CN 110586220A CN 201910982678 A CN201910982678 A CN 201910982678A CN 110586220 A CN110586220 A CN 110586220A
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- Prior art keywords
- seat plate
- liquid adding
- lifting seat
- rotating shaft
- sample
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0289—Apparatus for withdrawing or distributing predetermined quantities of fluid
- B01L3/0293—Apparatus for withdrawing or distributing predetermined quantities of fluid for liquids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
- B01L9/527—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for microfluidic devices, e.g. used for lab-on-a-chip
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention relates to the technical field of microfluidics, in particular to a liquid adding and sampling device for a microfluidic chip. Comprises a workbench, and a chip clamping mechanism, a liquid feeding mechanism, a sample feeding mechanism and a driving mechanism which are arranged on the workbench; the actuating mechanism includes the rotatable pivot that sets up on the workstation and is used for driving pivot pivoted step motor, fixed cam and the driving lever of being equipped with in the pivot, the cam is used for pushing down the lift bedplate along with the pivot rotation, the driving lever is used for pushing down the pressure head along with the pivot rotation, cam and driving lever staggered distribution, and be used for on the cam to have one section concentric arc section with the central concentric distribution of pivot with lift bedplate complex position, so that the driving lever rotates along with the pivot and pushes down the in-process that the pressure head was passed, highly maintain unchangeably through the sliding fit between concentric arc section and the lift bedplate in order to control the lift bedplate. The invention has simple structure and high reliability, and ensures the effective and efficient implementation of the microfluidic detection.
Description
Technical Field
The invention relates to the technical field of microfluidics, in particular to a liquid adding and sampling device for a microfluidic chip.
Background
The micro-fluidic chip is a new technology for carrying out automatic experiments and analysis by shrinking the biological and chemical experimental processes to a chip of a few centimeters. The key for realizing automatic detection by the microfluidic technology is the automation of sample adding and liquid feeding. The sample adding and liquid inlet device for the existing microfluidic chip is mostly operated manually, for example, patent 201620763359.9 discloses a liquid inlet clamp for the microfluidic chip, which adopts manual clamping to feed liquid and can not adapt to the requirements of microfluidic detection automation equipment. And the existing microfluidic sample adding and liquid feeding system has complex structure and operation, and poor stability and precision of sample adding and liquid feeding.
Disclosure of Invention
The invention aims to provide a liquid adding and sampling device for a microfluidic chip, which has a simple structure and high reliability and ensures effective and efficient implementation of microfluidic detection.
In order to solve the technical problems, the invention adopts the technical scheme that: a liquid feeding and sample feeding device for a microfluidic chip comprises a workbench, and a chip clamping mechanism, a liquid feeding mechanism, a sample feeding mechanism and a driving mechanism which are arranged on the workbench;
the liquid adding mechanism comprises two sliding columns which are vertically fixed on the workbench and are respectively positioned at two sides of the chip clamping mechanism, a lifting seat plate of which the end parts are respectively arranged on the two sliding columns in a sliding manner, and a plurality of liquid adding joints which are arranged on the lifting seat plate in a matching manner; the upper end of the liquid feeding joint is connected with a reagent pump through a hose, the lower end of the liquid feeding joint penetrates through the lower edge of the lifting seat plate and corresponds to a liquid feeding port on the chip up and down, a second reset spring is sleeved on the part of the liquid feeding joint located in the assembling hole, and two ends of the second reset spring are respectively in contact fit with a first bulge arranged on the lifting seat plate and a second bulge arranged on the liquid feeding joint;
the sample feeding mechanism comprises a sample feeder detachably connected to a sample inlet of the chip, a fixed seat plate fixedly arranged on the workbench and positioned above the chip clamping mechanism, and a pressure head arranged on the fixed seat plate in a sliding fit manner along the vertical direction, the sample feeder comprises a sample feeding pipe used for being connected with the sample inlet and a push rod which is arranged in the sample feeding pipe in a sliding manner and can push a sample in the sample feeding pipe into the sample inlet, an assembly groove for the sliding fit of the pressure head is formed in the fixed seat plate, the lower end of the pressure head extends out of a first through hole formed in the bottom of the assembly groove and can be in contact fit with the top of the push rod, a third reset spring is sleeved on a part of the pressure head positioned in the assembly groove, and two ends of the third reset spring are respectively in contact fit with a third bulge arranged at the top of the pressure head and the upper edge;
the actuating mechanism includes the rotatable pivot that sets up on the workstation and is used for driving pivot pivoted step motor, fixed cam and the driving lever of being equipped with in the pivot, the cam is used for pushing down the lift bedplate along with the pivot rotation, the driving lever is used for pushing down the pressure head along with the pivot rotation, cam and driving lever staggered distribution, and be used for on the cam to have one section concentric arc section with the central concentric distribution of pivot with lift bedplate complex position, so that the driving lever rotates along with the pivot and pushes down the in-process that the pressure head was passed, highly maintain unchangeably through the sliding fit between concentric arc section and the lift bedplate in order to control the lift bedplate.
Preferably, the lifting seat plate, the fixed seat plate and the rotating shaft are distributed in parallel at intervals.
Preferably, two cams in the same direction are respectively and fixedly arranged on the rotating shaft corresponding to the two ends of the lifting seat plate.
Preferably, the upper edge of the lifting seat plate is fixedly provided with a cover plate, a second through hole for the upper end of the liquid feeding connector to penetrate out is correspondingly formed in the position, corresponding to any one assembling hole, of the cover plate, and a first protrusion for the upper end of the second return spring to contact and match is formed in the part, located around the second through hole, of the cover plate.
Preferably, the two sides of the chip clamping mechanism on the workbench are respectively provided with an upright post for connecting the fixed seat plate and shaft seats for rotatably matching two ends of the rotating shaft.
Preferably, the groove wall of the assembly groove is provided with a strip-shaped notch for the deflector rod to extend into the assembly groove to be in contact fit with the top of the pressure head.
Preferably, the chip clamping mechanism comprises an L-shaped limiting plate and a spring clamping seat which are fixed on the workbench at intervals.
Preferably, the lower end of the push rod is provided with a sealing ring which is in sealing fit with the inner wall of the sampling tube.
Preferably, the lower ends of the liquid feeding joint and the sampling pipe are both conical.
Advantageous effects
The liquid adding head and the sample injector are driven by the same rotating shaft in cooperation with the stepping motor, so that the reagents and the samples can be accurately added. The cam for controlling the butt joint of the liquid adding joint and the chip and the deflector rod for controlling the sample injector to push the sample are arranged on the rotating shaft in a staggered mode, and liquid adding and sample injection can be controlled to be carried out in steps. Meanwhile, the cam is provided with a concentric arc section concentric with the rotating shaft, so that the situation that the rotating shaft rotates to press the pressure head down through the driving lever in the sample injection process is guaranteed, the height positions of the liquid adding joints are consistent with the lifting seat plate all the time and are normally matched with liquid adding ports on the chip, liquid adding can be normally supplied to the chip by the reagent pump, the liquid adding amount is controlled by the reagent pump, the sample injection amount is controlled by the rotating angle of the rotating shaft driven by the stepping motor, the liquid adding amount and the sample injection process are not interfered with each other, and the cam has the characteristics of simple structure, high reliability, easiness in automatic detection and wide application range.
Drawings
FIG. 1 is a schematic perspective view of the present invention in use;
FIG. 2 is a schematic diagram of the structure of FIG. 1 with the chip removed;
FIG. 3 is a schematic perspective view of the sample injector of the present invention in a state of being mounted with a chip;
FIG. 4 is a schematic diagram of the sample injector of the present invention;
FIG. 5 is a schematic view of the engagement between the liquid filling joint and the chip in the present invention;
FIG. 6 is a schematic diagram of the sample injector and the chip of the present invention;
the labels in the figure are: 1. the lifting seat plate comprises a lifting seat plate, 101, an assembling hole, 2, a cover plate, 3, a liquid adding connector, 4, a deflector rod, 5, a rotating shaft, 6, a cam, 601, a concentric arc section, 7, a sliding column, 8, a first return spring, 9, a shaft seat, 10, a belt pulley, 11, a fixed seat plate, 12, an upright column, 13, a pressure head, 14, a third return spring, 15, an assembling groove, 16, a spring clamping seat, 17, a sample injector, 1701, a push rod, 1702, a sealing ring, 1703, a sample inlet pipe, 18, a chip, 1801, a positioning notch, 1802, a liquid adding port, 1803, a sample inlet, 19, a workbench, 20, an L-shaped limiting plate, 21, a first bulge, 22, a second return spring, 23, a second bulge, 24 and a third bulge.
Detailed Description
As shown in fig. 1 and fig. 2, the liquid adding and sample feeding device for a microfluidic chip of the present invention comprises a worktable 19, and a chip clamping mechanism, a liquid adding mechanism, a sample feeding mechanism and a driving mechanism which are arranged on the worktable 19. The chip 18 is positioned and fixed on the table top of the worktable 19 through the chip clamping mechanism, and under the driving action of the driving mechanism, the liquid adding mechanism and the sample feeding mechanism sequentially inject the reagent and the sample into the chip 18. Reagent and sample injection are accurate and stable, do not interfere with each other, and the operation is convenient.
The chip clamping mechanism comprises an L-shaped limiting plate 20 and a spring clamping seat 16 which are fixed on the table top of the workbench 19 at intervals. The L-shaped limiting plate 20 and the spring clamping seat 16 are distributed in parallel at intervals. As shown in fig. 3, a positioning notch 1801 is provided at a distal end of the chip 18, and in a process of inserting the chip 18 into the L-shaped limiting plate 20 and the spring seat 16, the positioning notch 1801 is matched with the spring seat 16 to realize a function of positioning by pushing the chip 18 once and ejecting the chip 18 by pushing twice. The spring card seat 16 is the same as the SD card positioning and fixing mechanism in the prior art, and is not described herein.
The liquid adding mechanism mainly comprises a lifting seat plate 1 which is arranged above the chip clamping mechanism in a lifting way and a plurality of liquid adding joints 3 arranged on the lifting seat plate 1.
The lifting seat plate 1 is strip-shaped, and two ends of the lifting seat plate are respectively arranged on two sliding columns 7 which are vertically fixed on the workbench 19 and are positioned at two sides of the chip clamping mechanism in a sliding fit mode. The part of the sliding column 7 above the lifting seat plate 1 is locked by a nut, and the part below the lifting seat plate 1 is sleeved with a first return spring 8. The first return spring 8 is used for jacking the lifting seat plate 1 to the initial height by the elastic potential energy accumulated by the first return spring 8 after the lifting seat plate 1 is lowered to a certain height under the action of external force and the action of the external force disappears.
The liquid adding joints 3 are correspondingly inserted into a plurality of assembling holes 101 distributed along the length direction of the lifting seat plate 1 respectively. As shown in fig. 5, the fitting hole 101 is formed in a shape of a stepped hole having a large top and a small bottom, and penetrates the thickness of the lifting seat plate 1 in the vertical direction. The upper end of the liquid adding connector 3 is connected with a reagent pump through a hose (both the hose and the reagent pump are shown in the figure), and the lower end of the liquid adding connector 3 passes through a pin hole in the assembling hole 101 and then vertically corresponds to a liquid adding opening 1802 positioned and fixed on a chip 18 in the chip clamping mechanism. The part of the charging connector 3 in the large hole of the assembly hole 101 is sleeved with a second return spring 22. Two ends of the second return spring 22 are respectively in contact fit with a first protrusion 21 arranged on the lifting seat plate 1 and a second protrusion 23 arranged on the liquid charging connector 3. In this embodiment, a strip-shaped cover plate 2 is correspondingly fixed to the upper edge of the lifting seat plate 1, and two ends of the cover plate 2 are fixed to the lifting seat plate 1 through screws respectively. A plurality of first through holes are formed in the cover plate 2 at positions corresponding to the positions of the fitting holes 101, and through which the liquid feeding joints 3 are exposed to be connected to the reagent pump through the hose. The aperture of the first penetration hole is smaller than that of the large hole portion of the fitting hole 101 so that the portion of the cap plate 2 located at the first penetration hole forms the first projection 21.
When the liquid adding connector 3 moves downwards under the action of the driving mechanism, the liquid adding connector 3 is in contact with the chip 18, the lifting seat plate 1 continues to move downwards for a certain distance, the second return spring 22 is compressed, and the lower end of the liquid adding connector 3 is tightly pressed with a liquid adding port 1802 on the chip 18 to realize sealing. The lower end of the liquid adding connector 3 is provided with a certain taper, and a liquid adding port 1802 on the chip 18 is provided with a chamfer angle, so that the liquid adding connector 3 is convenient to center and seal. The number of the liquid adding joints 3 can be adjusted according to the detection requirement of the microfluidic chip 18 (namely the number of the liquid adding holes on the microfluidic chip 18).
The sample injection mechanism comprises a sample injector 17 for collecting a sample and injecting the sample into the chip 18 and a pressure head 13 for uniformly, stably and accurately controlling the sample injector 17 to complete sample injection into the chip 18.
Referring to fig. 3, the sample injector 17 is in the form of a syringe, and includes a sample inlet tube 1803 connected to the sample inlet 1803, and a push rod 1701 slidably disposed in the sample inlet tube 1803 and capable of pushing the sample in the sample inlet tube 1803 into the sample inlet 1803. An annular groove is provided on the outer periphery of the lower end of the push rod 1701, and a seal ring 1702 is fitted in the annular groove.
As shown in fig. 5, the pressing head 13 is slidably disposed on the strip-shaped fixing seat plate 11 fixed to a position above the chip holding mechanism in the vertical direction. Two ends of the fixed seat plate 11 are respectively fixed on the workbench 19 through the upright post 12, and the middle position of the fixed seat plate 11 is provided with an assembly groove 15 for the pressure head 13 to slide. The lower end of the ram 13 protrudes through a first through hole provided at the bottom of the fitting groove 15 and is adapted to be brought into contact with the top of the pushing rod 1701 of the injector 17 inserted into the chip 18 of the chip holding mechanism. The part of the pressure head 13 in the assembling groove 15 is sleeved with a third return spring 14, and two ends of the third return spring 14 are respectively in contact fit with a third protrusion 24 arranged at the top of the pressure head 13 and the upper edge of the bottom of the assembling groove 15.
The driving mechanism comprises a rotating shaft 5 which is rotatably arranged on the workbench 19, a stepping motor which is used for driving the rotating shaft 5 to rotate, and a cam 6 and a shifting lever 4 which are fixed on the rotating shaft 5. Two shaft seats 9 which are respectively used for the rotation matching of the end parts of the rotating shaft 5 are fixedly arranged on the workbench 19. A belt wheel 10 is fixedly arranged at one end of the rotating shaft 5, and the belt wheel 10 is driven by a stepping motor (the stepping motor is not shown in the figure) through a transmission belt to rotate. The two cams 6 are fixed at the two ends of the rotating shaft 5 in the same direction and used for pressing the lifting seat plate 1 down along with the rotation of the rotating shaft 5 so that the liquid adding connector 3 on the lifting seat plate 1 is in butt joint with the liquid adding port 1802 on the chip 18; the number of the deflector rods 4 is one and fixed in the middle of the rotating shaft 5, and the deflector rods 4 are used for pressing the pressure head 13 down along with the rotation of the rotating shaft 5, so that the pressure head 13 presses the pushing rod 1701 of the sample injector 17 down to inject the sample in the sample inlet 1803 of the sample injector 17 into the sample inlet 1803 of the chip 18.
Because the liquid adding and sample feeding processes are realized by the rotation drive of the rotating shaft 5, the cams 6 and the deflector rods 4 are distributed in a staggered way in order to avoid mutual interference. In the process of rotating along with the rotating shaft 5, the cam 6 is firstly contacted with the lifting seat plate 1, so that the lifting seat plate 1 descends along with the continuous rotation of the cam 6, and the lower end of the liquid adding connector 3 is butted with the liquid adding port 1802. After the sample is connected, the deflector rod 4 is contacted with the pressure head 13 along with the continuous rotation of the rotating shaft 5, so that the pressure head 13 descends along with the continuous rotation of the deflector rod 4, and the sample injection is completed. Meanwhile, in order to avoid that the lifting seat plate 1 also continuously descends in the sample feeding process, so that the liquid feeding connector 3 is excessively contacted with the butt joint port and even the liquid feeding connector 3 is broken, a concentric arc section 601 which is concentrically distributed with the center of the rotating shaft 5 is arranged at the position, matched with the lifting seat plate 1, on the cam 6. After the concentric arc 601 contacts the lifting seat plate 1, even if the rotating shaft 5 and the cam 6 rotate continuously, the height of the lifting seat plate 1 is kept unchanged all the time, so that the lower end of the liquid adding connector 3 is not damaged due to excessive contact.
The application process of the invention in the microfluidic experiment is as follows:
after the sample to be measured is collected by the sample injector 17, the sample is inserted into the chip 18 and inserted into the chip holding mechanism together with the chip 18. After the chip 18 is positioned, the belt wheel 10 is driven by a stepping motor to drive the rotating shaft 5 to rotate, and the rotation of the rotating shaft 5 is controlled to be divided into three stages:
(1) when the stepping motor is started (liquid adding), the rotating shaft 5 rotates by a certain angle from the initial position to drive the cam 6 to contact with the lifting seat plate 1, and the lifting seat plate 1 is pressed down by a certain distance to enable the liquid adding connector 3 to be in close contact with the liquid adding port 1802 on the chip 18. The other end of the liquid adding joint 3 is connected with a reagent pump, and reagents can be pumped in sequence according to the requirements of the detection process.
(2) The control motor is started for the second time (sample adding), the rotating shaft 5 continues to rotate for a certain angle, the shifting rod 4 is in contact with the pressure head 13 and performs downward pressing movement, the sample injector 17 fixed on the chip 18 is arranged below the pressure head 13, and the pressure head 13 pushes the sample injector 17 to complete sample injection. At this time, the pressing distance of the lifting seat plate 1 is kept unchanged when the rotating shaft 5 rotates within a certain range under the control of the concentric arc sections 601 on the cam 6. In order to ensure the stable pressing movement, a third return spring 14 is arranged in the pressing head 13.
(3) The stepping motor is started for three times to do reverse motion (reset), so that the deflector rod 4 is sequentially separated from the contact with the pressure head 13, the cam 6 and the lifting seat plate 1, the liquid feeding connector 3 and the pressure head 13 respectively return to the initial positions under the action of the first reset spring 8, the second reset spring 22 and the third reset spring 14.
Claims (9)
1. The utility model provides a micro-fluidic chip is with liquid feeding and sampling device which characterized in that: comprises a workbench (19), and a chip clamping mechanism, a liquid adding mechanism, a sample feeding mechanism and a driving mechanism which are arranged on the workbench (19);
the liquid adding mechanism comprises two sliding columns (7) which are vertically fixed on the workbench (19) and are respectively positioned at two sides of the chip clamping mechanism, a lifting seat plate (1) of which the end parts are respectively arranged on the two sliding columns (7) in a sliding manner, and a plurality of liquid adding joints (3) which are arranged on the lifting seat plate (1) in a matching manner; a first reset spring (8) for resetting the lifting seat plate (1) is sleeved on the sliding column (7), a liquid adding connector (3) is inserted into an assembly hole (101) which is vertically penetrated through the lifting seat plate (1), the upper end of the liquid adding connector (3) is connected with a reagent pump through a hose, the lower end of the liquid adding connector (3) penetrates out of the lower edge of the lifting seat plate (1) and vertically corresponds to a liquid adding port (1802) on a chip (18), a second reset spring (22) is sleeved on the part of the liquid adding connector (3) located in the assembly hole (101), and two ends of the second reset spring (22) are respectively in contact fit with a first bulge (21) arranged on the lifting seat plate (1) and a second bulge (23) arranged on the liquid adding connector (3);
the sample injection mechanism comprises a sample injector (17) detachably connected to a sample injection port (1803) of a chip (18), a fixed seat plate (11) fixedly arranged on a workbench (19) and positioned above the chip clamping mechanism, and a pressure head (13) installed on the fixed seat plate (11) in a sliding fit manner along the vertical direction, the sample injector (17) comprises a sample injection pipe (1703) connected with the sample injection port (1803) and a push rod (1701) which is arranged in the sample injection pipe (1703) in a sliding manner and can push a sample in the sample injection pipe (1703) into the sample injection port (1803), an assembly groove (15) for the sliding fit of the pressure head (13) is arranged on the fixed seat plate (11), the lower end of the pressure head (13) extends out of a first through hole arranged at the bottom position of the assembly groove (15) and can be in contact fit with the top of the push rod (1701), a third return spring (14) is sleeved on the part of the pressure head (13) positioned in the assembly groove (15), two ends of a third return spring (14) are respectively in contact fit with a third bulge (24) arranged at the top of the pressure head (13) and the upper edge of the bottom of the assembling groove (15);
the driving mechanism comprises a rotating shaft (5) rotatably arranged on the workbench (19) and a stepping motor used for driving the rotating shaft (5) to rotate, a cam (6) and a shifting lever (4) are fixedly arranged on the rotating shaft (5), the cam (6) is used for pressing down the lifting seat plate (1) along with the rotation of the rotating shaft (5), the shifting lever (4) is used for pressing down a pressure head (13) along with the rotation of the rotating shaft (5), the cam (6) and the shifting lever (4) are distributed in a staggered mode, a concentric arc section (601) which is concentrically distributed with the center of the rotating shaft (5) is arranged at the position, matched with the lifting seat plate (1), on the cam (6), so that the shifting lever (4) rotates along with the rotating shaft (5) to press down the pressure head (13), and the height of the lifting seat plate (1) is kept unchanged through sliding fit between the concentric arc section (601) and the lifting seat plate (1.
2. The liquid adding and sampling device for the microfluidic chip according to claim 1, wherein: the lifting seat plate (1), the fixed seat plate (11) and the rotating shaft (5) are distributed in parallel at intervals.
3. The liquid adding and sampling device for the microfluidic chip according to claim 1, wherein: two homodromous cams (6) are respectively and fixedly arranged on the rotating shaft (5) corresponding to the positions of the two ends of the lifting seat plate (1).
4. The liquid adding and sampling device for the microfluidic chip according to claim 1, wherein: the upper edge of the lifting seat plate (1) is fixedly provided with a cover plate (2), a second through hole for the upper end of the liquid adding connector (3) to penetrate out is correspondingly formed in the position of the cover plate (2) corresponding to any one assembling hole (101), and the part of the cover plate (2) positioned around the second through hole forms a first bulge (21) for the upper end of a second reset spring (22) to be in contact fit with.
5. The liquid adding and sampling device for the microfluidic chip according to claim 1, wherein: an upright post (12) for connecting a fixed seat plate (11) and shaft seats (9) for rotatably matching two ends of the rotating shaft (5) are respectively arranged on the two sides of the chip clamping mechanism on the workbench (19).
6. The liquid adding and sampling device for the microfluidic chip according to claim 1, wherein: and a strip-shaped notch for the deflector rod (4) to extend into the assembly groove (15) to be in contact fit with the top of the pressure head (13) is formed in the groove wall of the assembly groove (15).
7. The liquid adding and sampling device for the microfluidic chip according to claim 1, wherein: the chip clamping mechanism comprises an L-shaped limiting plate (20) and a spring clamping seat (16) which are fixed on the workbench (19) at intervals.
8. The liquid adding and sampling device for the microfluidic chip according to claim 1, wherein: the lower end of the push rod (1701) is provided with a sealing ring (1702) which is in sealing fit with the inner wall of the sampling tube (1703).
9. The liquid adding and sampling device for the microfluidic chip according to claim 1, wherein: the lower ends of the liquid adding joint (3) and the sampling tube (1703) are both conical.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910982678.7A CN110586220B (en) | 2019-10-16 | 2019-10-16 | Liquid feeding and sampling device for microfluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910982678.7A CN110586220B (en) | 2019-10-16 | 2019-10-16 | Liquid feeding and sampling device for microfluidic chip |
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| CN110586220A true CN110586220A (en) | 2019-12-20 |
| CN110586220B CN110586220B (en) | 2023-07-04 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113368918A (en) * | 2021-06-21 | 2021-09-10 | 合肥瀚海星点生物科技有限公司 | Multi-channel liquid separation device and method based on microfluidic printing |
| CN116536155A (en) * | 2023-05-17 | 2023-08-04 | 扬州大学 | Chip culture device and application method thereof |
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| CN116536155B (en) * | 2023-05-17 | 2024-04-09 | 扬州大学 | Chip culture device and application method thereof |
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