KR20100056291A - Sequential injection analyzer with replaceable lab-on-a-chip - Google Patents

Abstract

PURPOSE: A sequential injection analyzer is provided to easily exchange a fluid pipeline module, and to move or transport the analyzer with a simple and small structure compare to a conventional analyzer. CONSTITUTION: A sequential injection analyzer comprises the following: a detachable fluid pipeline module(260); and a syringe pump and a multiport valve fixed to a manifold(261), the fluid pipeline module is a laminating structure including substrates with minute flow paths. The fluid pipeline module performs a sequential injection analysis while pressured and coupled to the manifold. The detachable fluid pipeline module is formed with glass, silicon, polymethylmethacrylate, polycarbonate, polystyrene resins and others. A base substrate is connected with the manifold while including a penetration hole.

Description

Sequential injection analyzer with replaceable lab piping on replaceable lab-on-a-chip

The present invention relates to a chemical analysis device for measuring the concentration of a specific component in an aqueous solution. The flow injection analysis device is a representative method of automating wet chemical analysis. It automatically performs sample suction, quantification injection, mixing, reaction, and detection process to reduce errors that are likely to occur in manual operation, and performs repetitive work reliably. A further development of the flow injection analysis device is the sequential injection analysis device. Unlike the continuous flow of reagent and carrier solution, the sequential injection analyzer uses a multiport valve to inhale reagent and sample sequentially to react in the pipeline and transfer the reactants to the detector using the multiport valve. to be. The present invention is an improvement of the sequential injection analysis device.

 Flow injection analysis was pioneered by Lujika and Hanson in the 1970s, and thousands of papers and professional societies have been published over the past 30 years, and analysis methods have been reported for hundreds of chemicals. It is widely used for urine analysis and toxic substance analysis. The sequential injection analysis device, which is an improved flow injection analysis method, consists of a syringe pump and a multiport valve. Select the sample and reagent by using the multiport valve, and inject the reagent and sample sequentially with a syringe pump to react in the tube.After the reaction, select the multiport valve toward the detector and transfer the reactants to the detector. Detect. The main advantages of the sequential injection analyzer are the low reagent consumption and the simple operation. However, there is a disadvantage that an expensive multiport valve and a syringe pump are not required in the flow injection analysis method.

Sequential injection analysis was devised by the University of Washington team in Seattle, USA, and was widely used in the analytical chemistry world. fialab.com originates from a team that designed sequential analyzers and offers a variety of sequential injectors. In particular, the lab-on-a-valve structure is currently evaluated as the most advanced system in the sequential injection method, and is easy to manufacture and has many advantages in miniaturization. The pipe was simplified by arranging the detector, the mixing coil, and the syringe pump around the multiport valve (http://www.flowinjection.com/Brochures/fialab3200.aspx).

However, these wrap language valve structure is difficult to implement the mechanical process and still not removable removable structure that the present invention seeks.

Similarly to the present invention, there is a lab language chip that seeks miniaturization (http://en.wikipedia.org/wiki/Lab-on-a-chip). Lap language chips have a structure in which pumps, valves, and flow paths are integrated into a small laminated substrate, and are being spotlighted with advanced technology. However, the fluid driving method of the lab language chip uses an electroosmotic force using high pressure electricity. For this purpose, the flow path must be very fine, and the detector must also detect the sample in the fine flow path, so that high-precision and expensive equipment such as laser induction fluorescence is required. The disadvantage is that it is necessary. Although the chip can be small, miniaturization is not easy given the detection devices and other electronic devices.

One of the difficulties in making and using sequential injection analysis is the piping problem. The most basic device consists of a six-port valve, a syringe pump, and a detector. For example, there are twelve pipe inlets and outlets, and the tubing must be connected with special expensive fittings in order to pipe without dead volume. When using the device, if the inner wall of the pipe is contaminated, scaled, or replaced with a different type of reagent, the pipe must be changed accordingly. On the other hand, when the piping work with the fittings and tubes, the volume occupies also increases, which increases the size of the device.

Recently, as sequential injection analysis devices are used in biotechnology such as water analysis and food analysis, such as immunoassay, there is a need to use very few samples or reagents.

In addition, there are many protein measurements in the field of biotechnology, and since proteins adhere well to the flow path walls, there is a difficulty in frequently replacing pipes.

For these applications, pipes made of conventional fittings and tubes are not suitable because of their large internal volume, which leads to the need for a sequential analysis device that can simplify the flow path, simplify the pipe, and replace the entire pipe easily.

The present invention solved the problem of miniaturization and easy pipe replacement by devising to make the module detachable by detaching the pipe. The syringe pump and the multi-port valve are all adjusted to the manifold, and the input and output parts of the valve and the pump are tightly connected to the input / output part of the piping module by Teflon or O-ring so that they can be combined without leakage. The problem of integrating a piping unit and modularizing it was solved by using a lab language chip technology using an acrylic resin. This technology engraves the desired pipe shape on the base acrylic substrate by various techniques such as milling, hot stamping, compression molding, injection molding, and laser processing, and covers and bonds the cover substrate on it. The micro-channel is characterized in that there is no deformation or blockage.

The flow injection analyzer according to the present invention has no cumbersome piping tubes or fittings compared to the existing apparatus, so the size is very small, so it is easy to move or carry. It is convenient for detecting stuck samples, and the flow path engraved in the piping module can be made very precisely, and can be used for biotechnological applications where the amount of sample or reagent is very small.

It will be described with reference to the drawings. 1 is a functional diagram of a typical sequential injection analysis device. First, the operation sequence of sequential injection analysis will be explained. First, the sample 154, the reagent 1 106, and the reagent 2 107 are provided, and the tubing pump 110 is operated to suck the sample and to discharge it to the outlet. During this process, the multi-port valve 103 is positioned at the position where the sample is selected, and the syringe pump 109 is positioned at the right of the built-in three-way valve 102 to inhale the sample and inject it into the mixing coil 102. . At the end of the sample injection, turn off the tubing pump and the multiport valve grows to the position of reagent 1 (106). The syringe pump then inhales reagent 1 and injects it into the mixing coil. Since the sample is first injected into the mixed coil, the reagent 1 is sequentially injected. In the same manner, reagent 2 107 may also be injected after reagent 1. After a certain time, the reaction is carried out by mixing the reagent and the sample under the influence of diffusion. When the reaction is completed, the multi-port valve is set to the detector 108 position, the built-in three-way valve of the syringe pump is extended to the left to pump the carrier solution and filled into the syringe, and then the three-way valve is turned to the right side. The reaction solution is transferred to the detector in one carrier solution. The detector measures the concentration of the reactants by methods such as absorption, emission, fluorescence, and electrochemical electrodes.

Next, an embodiment of a sequential injection analyzer adopting a multi-port valve having one 6 port will be described with reference to FIG. 2 for a piping module which is the core of the present invention.

The sample inlet 204 is connected to the tubing pump and the sample outlet 205 is connected to the waste medicine container. The reagent inlet 1 206, the reagent inlet 2 207, the reagent inlet 3 208, and the reagent inlet 4 209 are respectively connected to the corresponding reagent bottles. The syringe portion of the syringe pump is connected to the syringe port 201, the three-way valve of the syringe pump is connected to the three-way valve port 211, the six-port valve is connected to the six-port valve port 202. The piping module consisted of two layers in this embodiment. The base substrate 224 is bonded to the cover substrate 221 by thermal diffusion method. On the bottom of the base board or the bottom of the cover board, fine flow paths are formed by milling, hot stamping, compression molding, injection molding, laser processing, etc. 223 and 6-port valve port 225 are respectively in close contact with the syringe, 3-way valve, 6-port valve fixed to the manifold.

FIG. 3 is a diagram illustrating how the pipe module is in close contact with a fluid control device such as a syringe, a 3-way valve, a 6-port valve, and the like fixed to a manifold. The syringe pump is composed of parts 236 and 237 which change the rotational motion of the syringe 238 and the motor into linear motion. Meanwhile, the syringe pump works in conjunction with the 3-way valve. The three-way valve is composed of a Teflon disk 233, a Teflon backup plate 234, and a motor 235 for rotating the backup plate. Teflon discs are engraved with slotted slots and are fitted with three vertical through holes. When the teflon disk is rotated by 90 degrees, it is connected with the two in the through hole (240), and when it is rotated back to the 9th position, it is connected with the two or four in the through hole (241).

4 shows how the piping module 260 and the manifold 261 are detached from each other. Through-holes for the three-way valve port in the piping module is in close contact with the Teflon disk 262 of the manifold, the through-hole for the syringe port of the piping module is in close contact through the O-ring 263 of the manifold. No leakage occurs during compression due to the elasticity of the Teflon or O-ring material, and Teflon Sysk can be rotated at the same time while being compressed without leakage due to self-lubrication.

It will now be described by looking at the drawings for an embodiment of applying a sequential injection analyzer having a removable piping module according to the present invention to a portable water analyzer.

The present invention is particularly suitable for portable field water analyzers. Figure 5 is a photograph of the finished product applied to the nitric acid / nitrite ion analyzer.

The chemical reaction used is as follows.

Nitrate -------> Nitrite + Sulfanylamide Reagent-> 540nm Wavelength Absorbance Measurement

       Cadmium reducing agent

Recently, groundwater has become a problem due to contamination with nitrates. A standard method of water quality analysis was performed using a method of measuring chromium ions to nitrite ions and reacting nitrite ions with sulfanylamide reagents using an absorbance meter at 540 nm.

6, the groundwater sample 272 is prepared, and the sulfanyl amide reagent 271 is connected to the six-port valve port, and the cadmium powder is put in a long narrow container and connected to the six-port valve port. The sample is aspirated, transferred to a cadmium powder container, and the pump is stopped for a certain period of time. After the reaction is completed, remove the sample from the cadmium container, transfer to the mixed coil and inhale the sulfanylamide reagent to react. After the reaction, the reactant is sent to a detector, which is measured at 540 nm with an absorbometer (273). In the present embodiment, the piping module was manufactured in a size of 52 mm x 45 mm x 6 mm, and the manifold was manufactured in a size of 86 mm x 50 mm x 45 mm.

1 is a functional diagram of a sequential injection analyzer.

2 is a plan view of the piping module.

3 is a front view of a shape in which the piping module and the manifold are tightly coupled.

4 is a diagram showing the role of Teflon and O-ring in tight coupling between the piping module and the manifold.

5 is a photograph of an embodiment.

FIG. 6 is a functional diagram of an example of a Zr acid / nitrite ion analyzer. FIG.

Claims (2)

As a sequential injection analysis device It has a removable piping module, the syringe pump and the multi-port valve is fixed to the manifold, the piping module is a laminated structure of the substrate formed with a fine flow path, the piping module is press-bonded to the manifold to perform sequential injection analysis Sequential injection analysis device, characterized in that the pipe module can be removed and replaced According to claim 1, The piping module is a laminated structure consisting of glass, silicon, polymethyl methacrylate, polycarbonate, polystyrene resin, etc., the base substrate has a through hole connected to the manifold, each substrate has a fine flow path Sequential injection analysis device is formed and each substrate has a through hole to connect the flow path of the upper and lower substrates

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