CN205538412U - Be used for purified sample homogenate of RNA to remove DNA device simultaneously - Google Patents
Be used for purified sample homogenate of RNA to remove DNA device simultaneously Download PDFInfo
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- CN205538412U CN205538412U CN201520988133.4U CN201520988133U CN205538412U CN 205538412 U CN205538412 U CN 205538412U CN 201520988133 U CN201520988133 U CN 201520988133U CN 205538412 U CN205538412 U CN 205538412U
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- 239000012521 purified sample Substances 0.000 title abstract 2
- 239000012528 membrane Substances 0.000 claims abstract description 21
- 238000000265 homogenisation Methods 0.000 claims description 13
- 238000000746 purification Methods 0.000 claims description 12
- 239000000523 sample Substances 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 11
- 238000001179 sorption measurement Methods 0.000 abstract 4
- 230000029087 digestion Effects 0.000 abstract 1
- 239000013614 RNA sample Substances 0.000 description 6
- 238000000605 extraction Methods 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
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Abstract
The utility model discloses a be used for purified sample homogenate of RNA to remove DNA device simultaneously, the main part of device is a centrifuging tube structure, be equipped with a plurality of functional membranes in the centrifuging tube, the functional membrane includes two homogenate membranes and a DNA adsorption film, two homogenate membranes are first homogenate membrane and second homogenate membrane respectively, first homogenate membrane, second homogenate membrane and DNA adsorption film are from top to bottom located in proper order in the centrifuging tube, wherein, first homogenate membrane is close to the mouth of pipe setting of centrifuging tube, the DNA adsorption film is close to the socle setting of centrifuging tube, above -mentioned each functional membrane is all hugged closely the inner wall setting of centrifuging tube. The utility model discloses can realize homogenate and go one step of completion of DNA, first homogenate membrane and second homogenate membrane play the homogenate effect, and DNA adsorption film now plays the effect of adsorbing genome DNA, yield through the sample RNA of homogenate can increase, and use this device to save time, can not use the digestion of DNASE I to sparingly test the cost.
Description
Technical field
This utility model relates to a kind of RNA purification devices, concretely relates to that a kind of sample homogenization for RNA purification is double removes DNA device.
Background technology
Currently available technology is the genomic DNA during using DNASE I to remove extraction RNA, and DNASE I cost is high, and easily causes protein contamination, when again removing unnecessary Dnase I, also can lose a part of RNA, also cannot embody homogenate effect in extraction.
Summary of the invention
For the deficiencies in the prior art, the purpose of this utility model is to provide that a kind of sample homogenization for RNA purification is double removes DNA device, this device can realize homogenate and go DNA mono-step to complete, extraction process is without using DNASE I, improve the yield of RNA, also save experimental cost and experimental period simultaneously.
This utility model solution technical problem be the technical scheme is that a kind of sample homogenization for RNA purification is double and removed DNA device, the main body of described device is centrifuge tube body, described tubular body is provided with some functional membranes, described functional membrane includes that two homogenate films and a DNA adsorbed film, said two homogenate film are respectively the first homogenate film and the second homogenate film；Described first homogenate film, the second homogenate film and DNA adsorbed film are from top to bottom sequentially arranged in described body, and wherein, described first homogenate film is arranged near the mouth of pipe of described body, and described DNA adsorbed film is arranged near at the bottom of the pipe of described body；Each described functional membrane is all close to the inwall of described body and is arranged.
Further, the aperture of described first homogenate film is 20 microns, and the aperture of described second homogenate film is 100 microns.
Further, described first homogenate film, the second homogenate film and DNA adsorbed film are pellosil.
During use, joining in said apparatus by the RNA sample containing DNA, by centrifugal method, RNA sample plays homogenate effect through the first homogenate film and the second homogenate film, DNA in sample is adsorbed by the DNA adsorbed film in body, thus reaches homogenate and go DNA mono-step to complete；The solution filtered out through this device is the RNA sample without DNA.
The beneficial effects of the utility model are: compared with prior art, and the sample homogenization for RNA purification of this utility model offer is double removes DNA device, can realize homogenate and go DNA mono-step to complete, it is not necessary to just can reach the effect of homogenate again with other homogenization apparatus；First homogenate film and the second homogenate film play homogenate effect, and DNA adsorbed film below plays the effect of absorption genomic DNA；Can increase through the yield of sample RNA of homogenate, and use this device can be time-consuming, DNASE I can not be used to digest, to save experimental cost.
Accompanying drawing explanation
The double structural representation removing DNA device of the sample homogenization for RNA purification that Fig. 1 provides for this utility model.
1-body；2-first is homogenized film；3-second is homogenized film；4-DNA adsorbed film.
Detailed description of the invention
Below in conjunction with the accompanying drawings and detailed description of the invention, it is further elucidated with this utility model, it should be understood that following detailed description of the invention is merely to illustrate this utility model rather than limits scope of the present utility model.It should be noted that the word "front", "rear" used in describing below, "left", "right", "up" and "down" refer to the direction in accompanying drawing, word " interior " and " outward " refer respectively to the direction towards or away from particular elements geometric center.
As shown in Figure 1, a kind of sample homogenization for RNA purification is double removes DNA device, the main body of described device is a centrifuge tube body 1, described body 1 is internal is provided with some functional membranes, described functional membrane includes that two homogenate films and a DNA adsorbed film, said two homogenate film are respectively the first homogenate film 2 and the second homogenate film 3；Described first homogenate film 2, second is homogenized film 3 and DNA adsorbed film 4 and is from top to bottom sequentially arranged in described body 1, and wherein, described first homogenate film 2 is arranged near the mouth of pipe of described body 1, and described DNA adsorbed film 4 is arranged near at the bottom of the pipe of described body 1；Each described functional membrane is all close to the inwall of described body 1 and is arranged.
The aperture of described first homogenate film 2 is 20 microns, and the aperture of described second homogenate film 3 is 100 microns.The first described homogenate film 2, second is homogenized film 3 and DNA adsorbed film 4 and is pellosil.
During use, joining in said apparatus by the RNA sample containing DNA, by centrifugal method, RNA sample plays homogenate effect through the first homogenate film 2 and the second homogenate film 3, DNA in sample is adsorbed by the DNA adsorbed film 4 in pipe, thus reaches homogenate and go DNA mono-step to complete；The solution filtered out through this device is the RNA sample without DNA.
The sample homogenization for RNA purification of the present embodiment offer is double removes DNA device, can realize homogenate and go DNA mono-step to complete, it is not necessary to just can reach the effect of homogenate again with other homogenization apparatus；First homogenate film and the second homogenate film play homogenate effect, and DNA adsorbed film below plays the effect of absorption genomic DNA；Can increase through the yield of sample RNA of homogenate, and use this device can be time-consuming, DNASE I can not be used to digest, to save experimental cost.
The above embodiments are only to be described preferred implementation of the present utility model, are not defined spirit and scope of the present utility model.On the premise of without departing from this utility model design concept, various modification that the technical solution of the utility model is made by this area ordinary person and improvement, protection domain of the present utility model all should be dropped into.
Claims (3)
1. the sample homogenization for RNA purification is double removes DNA device, it is characterized in that: the main body of described device is centrifuge tube body, described tubular body is provided with some functional membranes, described functional membrane includes that two homogenate films and a DNA adsorbed film, said two homogenate film are respectively the first homogenate film and the second homogenate film；Described first homogenate film, the second homogenate film and DNA adsorbed film are from top to bottom sequentially arranged in described body, and wherein, described first homogenate film is arranged near the mouth of pipe of described body, and described DNA adsorbed film is arranged near at the bottom of the pipe of described body；Each described functional membrane is all close to the inwall of described body and is arranged.
A kind of sample homogenization for RNA purification is double removes DNA device, it is characterised in that: the aperture of described first homogenate film is 20 microns, and the aperture of described second homogenate film is 100 microns.
A kind of sample homogenization for RNA purification is double removes DNA device, it is characterised in that: described first homogenate film, the second homogenate film and DNA adsorbed film are pellosil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520988133.4U CN205538412U (en) | 2015-12-03 | 2015-12-03 | Be used for purified sample homogenate of RNA to remove DNA device simultaneously |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520988133.4U CN205538412U (en) | 2015-12-03 | 2015-12-03 | Be used for purified sample homogenate of RNA to remove DNA device simultaneously |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205538412U true CN205538412U (en) | 2016-08-31 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520988133.4U Active CN205538412U (en) | 2015-12-03 | 2015-12-03 | Be used for purified sample homogenate of RNA to remove DNA device simultaneously |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205538412U (en) |
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2015
- 2015-12-03 CN CN201520988133.4U patent/CN205538412U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190702 Address after: 311121 Room 101, Building 2636 Yuhangtang Road, Cangqian Street, Yuhang District, Hangzhou City, Zhejiang Province Patentee after: Hangzhou Beiwo Medical Technology Co., Ltd. Address before: 314000 2nd Floor, Building 10, 501 Changshengnan Road, Jiaxing City, Zhejiang Province Patentee before: Shi Jingyu |