CN206799616U - Purification column applied to plasmid extraction kit - Google Patents
Purification column applied to plasmid extraction kit Download PDFInfo
- Publication number
- CN206799616U CN206799616U CN201720470164.XU CN201720470164U CN206799616U CN 206799616 U CN206799616 U CN 206799616U CN 201720470164 U CN201720470164 U CN 201720470164U CN 206799616 U CN206799616 U CN 206799616U
- Authority
- CN
- China
- Prior art keywords
- filter membrane
- extraction kit
- plasmid extraction
- sample tube
- out sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A kind of purification column applied to plasmid extraction kit is the utility model is related to, is characterized in that：There are filter assemblies on the collecting pipe being detachably connected including adsorption column and with adsorption column, the top of the adsorption column with adding mouth, bottom with out sample tube mouth, the out sample tube mouth releasable connection.It can effectively remove the miscellaneous bacteria in product, need not be degerming again or using high concentration antibiotic treatment in subsequent experimental transfection process, so as to improve product pick-up rate and eliminate to resulting adverse effect.
Description
Technical field
Molecular biology or medicinal plasmid extracting technology field are the utility model is related to, it is more particularly to a kind of to be applied to plasmid
The purification column of extracts kit.
Background technology
Plasmid extraction is operation common in molecular biology and medicinal plasmid research field, and its product is usually used in follow-up reality
The processes such as the transfection tested, conversion, vector construction.In recent years, gene therapy and DNA vaccines are in cancer, single gene inheritance disease, Chinese mugwort
Prominent advantage is shown in terms of the diagnosis and treatment of growing the major diseases such as disease, angiocardiopathy, arthritis, its application study is just
It is growing.Plasmid is increasingly becoming the most important gene delivery system of gene therapy at present, applied to the medicinal of gene therapy
The development of the extractive technique of plasmid is at present as one of emphasis of the area research.This further improves high-purity plasmid to carry
Take the development of technology.
The method extraction matter that plasmid extraction kit common at present is isolated and purified using alkaline lysis combination plasmid purification column
Grain, the product after extraction are placed in sterilized water or TE solution and preserved.The patent text that existing Authorization Notice No. is CN201527375U
Part discloses a kind of nucleic acid and isolates and purifies post, including adsorption column, waste collection pipe, adsorption column are enclosed in waste collection pipe, absorption
Absorption column bottom is placed on, the buckle for fixing adsorption layer is provided with adsorption column.
Although this nucleic acid isolates and purifies post and fundamentally solves nucleic acid adsorption material pole during nucleic acid isolates and purifies
Caducous chronic disease, but there is no degerming measure during isolating and purifying, this results in product in the experiments such as follow-up transfection
Often need to carry out bacteriological filtration processing or use high concentration antibiotic treatment, so as to reduce product pick-up rate and produce unfavorable shadow to result
Ring.
The content of the invention
The purpose of this utility model is to provide a kind of purification column applied to plasmid extraction kit, and it can effectively be removed
Miscellaneous bacteria in product, need not be degerming again or using high concentration antibiotic treatment in subsequent experimental transfection process, so as to improve
Product pick-up rate and eliminate to resulting adverse effect.
Above-mentioned technical purpose of the present utility model technical scheme is that：
A kind of purification column applied to plasmid extraction kit, including adsorption column and the receipts that are detachably connected with adsorption column
There is filtering group on collector, the top of the adsorption column with adding mouth, bottom with out sample tube mouth, the out sample tube mouth releasable connection
Part.
By using above-mentioned technical proposal, filter assemblies are installed during isolating and purifying, filter assemblies can remove product
In miscellaneous bacteria, in subsequent experimental transfection process without it is degerming again or use high concentration antibiotic treatment, so as to improve production
Thing pick-up rate and eliminate to resulting adverse effect.
The utility model is further arranged to：The filter assemblies include the screen pipe being detachably connected with out sample tube mouth,
The bottom of the screen pipe is installed with filter membrane collet, and the filter membrane collet offers some vias, the upper table of the filter membrane collet
Face is disposed with filter membrane and filter membrane pressure ring, and the bottom of the filter membrane pressure ring and out sample tube mouth is inconsistent.
By using above-mentioned technical proposal, the filter membrane collet for being fixedly arranged on filtering bottom of the tube is used to accept filter membrane, prevents filter membrane
In centrifugal process, deviate from screen pipe；And filter membrane pressure ring sets and plays a part of protection to filter membrane, screen pipe and out sample tube are prevented
When mouth connects, the breakage of filter membrane is caused.
The utility model is further arranged to：The filter membrane pressure ring is flexible setting.
By using above-mentioned technical proposal, it is easy to being installed in screen pipe for filter membrane pressure ring.
The utility model is further arranged to：The bottom of the inwall of the screen pipe offers resigning annular groove, the filter membrane
Pressure ring includes the location division being adapted with resigning annular groove and the sealing inconsistent with out sample tube mouth.
By using above-mentioned technical proposal, location division, which is set, causes filter membrane pressure ring to be limited in screen pipe.
The utility model is further arranged to：External screw thread is provided with the outer wall of the out sample tube mouth, the screen pipe
Internal thread, the screen pipe and out sample tube mouth threaded connection are provided with inwall.
By using above-mentioned technical proposal, screw connection structure is simple, is easy to the installation and dismounting of screen pipe, and contributes to
Sealed connection between screen pipe and out sample tube mouth.
The utility model is further arranged to：The filter membrane is 0.22 μm of filter membrane.
By using above-mentioned technical proposal, efficient absorption plasmid and living contaminants therein can be removed.
The utility model is further arranged to：Some vias form honeycomb porous structure.
By using above-mentioned technical proposal, honeycomb porous structure is made up of the via of regular hexagon, and honeycomb porous structure is not only
Make filter membrane collet that there is enough structural strengths, and filter membrane collet is not hindered the outflow of liquid, save material.
The utility model is further arranged to：The top of the outer wall of the adsorption column is provided with bulge loop, the collecting pipe
Upper inside wall is provided with fin, bulge loop and the fin clamping.
By using above-mentioned technical proposal, the bulge loop for being arranged at adsorption column and the fin clamping for being arranged at collecting pipe can be realized
Being detachably connected between adsorption column and collecting pipe, and interface arrangment is simple, is easily installed and dismantles.
The utility model is further arranged to：The adsorption column is connected with the lid for sealing adding mouth by connect band
Body.
By using above-mentioned technical proposal, lid is provided for sealing adding mouth.
In summary, the utility model has the advantages that：Filter assemblies are installed during isolating and purifying, filtered
Component can remove the miscellaneous bacteria in product, need not be degerming again or using at high concentration antibiotic in subsequent experimental transfection process
Reason, so as to improve product pick-up rate and eliminate to resulting adverse effect.
Brief description of the drawings
Fig. 1 is the structural representation of embodiment
Fig. 2 is the line A-A sectional view in Fig. 1；
Fig. 3 is the B portions enlarged drawing in Fig. 2；
Fig. 4 is the configuration schematic diagram of embodiment；
Fig. 5 is the structural representation of screen pipe in embodiment.
In figure, 1, adsorption column；11st, adding mouth；12nd, out sample tube mouth；121st, external screw thread；13rd, bulge loop；14th, connect band；15、
Lid；2nd, collecting pipe；21st, fin；3rd, filter assemblies；31st, screen pipe；311st, internal thread；312nd, resigning annular groove；313rd, filter membrane bottom
Support；3131st, via；32nd, 0.22 μm of filter membrane；33rd, filter membrane pressure ring；331st, location division；332nd, sealing.
Embodiment
The utility model is described in further detail below in conjunction with accompanying drawing.
Embodiment, applied to the purification column of plasmid extraction kit, as Figure 1-5, including adsorption column 1, the and of collecting pipe 2
Filter assemblies 3.
Adsorption column 1 is internally provided with the sorbing material for being capable of efficient, single-minded absorption plasmid, and the top of adsorption column 1 has
Adding mouth 11, bottom have out sample tube mouth 12, and adsorption column 1 is connected with the lid for sealing adding mouth 11 by connect band 14
15.The top of the outer wall of adsorption column 1 is provided with bulge loop 13, and the upper inside wall of collecting pipe 2 is provided with fin 21, bulge loop 13 and fin
21 clampings, and then realize being detachably connected between adsorption column 1 and collecting pipe 2.
Filter assemblies 3 include 31,0.22 μm of filter membranes 32 of screen pipe and the filter membrane pressure ring 33 of flexible setting, the bottom of screen pipe 31
Portion is installed with filter membrane collet 313, and screen pipe 31 and filter membrane collet 313 are integrally formed setting, filter membrane collet 313 offer it is some just
The via 3131 of hexagon, these vias 3131 form honeycomb porous structure, and the inwall of screen pipe 31 is in close to filter membrane collet 313
One end offers resigning annular groove 312.And filter membrane pressure ring 33 includes the location division 331 being adapted with resigning annular groove 312 and with going out
The inconsistent sealing of sample ozzle 12, the location division 331 of filter membrane pressure ring 33 are installed in the resigning annular groove 312 of screen pipe 31, filter
The sealing of mould ring 33 and the inwall of filter membrane pressure ring 33 fit.Screen pipe 31 is in one end away from filter membrane collet 313
Internal thread 311 is offered on wall, external screw thread 121, screen pipe 31 and the spiral shell of out sample tube mouth 12 are provided with the outer wall of out sample tube mouth 12
Line connects, and then realizes the releasable connection between out sample tube mouth 12 and filter assemblies 3.When screen pipe 31 and the screw thread of out sample tube mouth 12
After connection, the sealing of out sample tube mouth 12 and filter membrane pressure ring 33 is inconsistent.
The operation principle that this is applied to the purification column of plasmid extraction kit is as follows：Adsorption column 1 is inserted in collecting pipe 2, and
Connected and fixed by the bulge loop 13 for being arranged at adsorption column 1 and the fin 21 for being arranged at collecting pipe 2, open lid 15, will be to bacterium
Culture carries out the liquid after cracking processing, is added to by adding mouth 11 in adsorption column 1, covers lid 15.Room temperature is placed, from
The heart, extract adsorption column 1 and abandon waste liquid, adsorption column 1 is put into collecting pipe 2, open lid 15, rinsing liquid is added into adsorption column 1,
Lid 15 is covered, is centrifuged, adsorption column 1 is extracted and abandons waste liquid.The opening of adsorption column 1 is placed in room temperature or 50 DEG C of incubators are placed several minutes
Afterwards, combined with screen pipe 31 in the collecting pipe 2 after being put into a sterilizing, to the hanging 1-2ml that is added dropwise in adsorbed film center through 65 DEG C of water
The eluent of preheating is bathed, room temperature is placed, centrifugation.
This specific embodiment is only that it is not to limitation of the present utility model, ability to explanation of the present utility model
Field technique personnel can make the modification of no creative contribution to the present embodiment as needed after this specification is read, but
As long as all protected in right of the present utility model by Patent Law.
Claims (9)
1. a kind of purification column applied to plasmid extraction kit, including adsorption column (1) and it is detachably connected with adsorption column (1)
Collecting pipe (2), the top of the adsorption column (1) exists with adding mouth (11), bottom with out sample tube mouth (12), its feature
In：Out sample tube mouth (12) releasable connection has filter assemblies (3).
2. the purification column according to claim 1 applied to plasmid extraction kit, it is characterised in that：The filter assemblies
(3) screen pipe (31) being detachably connected with out sample tube mouth (12) is included, the bottom of the screen pipe (31) is installed with filter membrane bottom
Hold in the palm (313), the filter membrane collet (313) offers some vias (3131), and the upper surface of the filter membrane collet (313) is set successively
Filter membrane and filter membrane pressure ring (33) are equipped with, the filter membrane pressure ring (33) and the bottom of out sample tube mouth (12) are inconsistent.
3. the purification column according to claim 2 applied to plasmid extraction kit, it is characterised in that：The filter membrane pressure ring
(33) it is flexible setting.
4. the purification column according to claim 3 applied to plasmid extraction kit, it is characterised in that：The screen pipe
(31) bottom of inwall offers resigning annular groove (312), and the filter membrane pressure ring (33) includes being adapted with resigning annular groove (312)
Location division (331) and the sealing (332) inconsistent with out sample tube mouth (12).
5. the purification column according to claim 2 applied to plasmid extraction kit, it is characterised in that：The out sample tube mouth
(12) external screw thread (121) is provided with outer wall, internal thread (311), the mistake are provided with the inwall of the screen pipe (31)
Chimney filter (31) and the out sample tube mouth (12) threaded connection.
6. the purification column according to claim 2 applied to plasmid extraction kit, it is characterised in that：The filter membrane is
0.22 μm of filter membrane (32).
7. the purification column according to claim 2 applied to plasmid extraction kit, it is characterised in that：Some vias
(3131) honeycomb porous structure is formed.
8. the purification column according to claim 1 applied to plasmid extraction kit, it is characterised in that：The adsorption column
(1) top of outer wall is provided with bulge loop (13), and the upper inside wall of the collecting pipe (2) is provided with fin (21), the bulge loop
And fin (21) clamping (13).
9. the purification column according to claim 1 applied to plasmid extraction kit, it is characterised in that：The adsorption column
(1) lid (15) for sealing adding mouth (11) is connected with by connect band (14).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720470164.XU CN206799616U (en) | 2017-04-28 | 2017-04-28 | Purification column applied to plasmid extraction kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720470164.XU CN206799616U (en) | 2017-04-28 | 2017-04-28 | Purification column applied to plasmid extraction kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206799616U true CN206799616U (en) | 2017-12-26 |
Family
ID=60739035
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720470164.XU Active CN206799616U (en) | 2017-04-28 | 2017-04-28 | Purification column applied to plasmid extraction kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206799616U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108728432A (en) * | 2018-06-08 | 2018-11-02 | 浙江大学 | The method and its application of efficient purified virus double-stranded RNA genome in plant tissue |
| CN115093937A (en) * | 2022-07-19 | 2022-09-23 | 南开大学 | Plasmid extractor and extraction method |
-
2017
- 2017-04-28 CN CN201720470164.XU patent/CN206799616U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108728432A (en) * | 2018-06-08 | 2018-11-02 | 浙江大学 | The method and its application of efficient purified virus double-stranded RNA genome in plant tissue |
| CN115093937A (en) * | 2022-07-19 | 2022-09-23 | 南开大学 | Plasmid extractor and extraction method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106754292B (en) | The single-stranded separators of DNA and separation method for pyrosequencing | |
| CN206799616U (en) | Purification column applied to plasmid extraction kit | |
| CN206315290U (en) | A kind of Chinese medicine extract isolates and purifies system | |
| CN206924902U (en) | One kind purifying enrichment facility | |
| CN109289307A (en) | A kind of method of plasticiser in removing extract | |
| CN111205979A (en) | RNA extraction device and method | |
| JP7264046B2 (en) | Genetic test method and genetic test kit | |
| CN111150755B (en) | Traditional Chinese medicine composition for preventing and treating viral diseases and application thereof | |
| CN210170984U (en) | Filter equipment of pharmacy extract | |
| CN102321613A (en) | Extraction method of clotting genomic DNA (deoxyribonucleic acid) | |
| CN209348425U (en) | Cryptosporidium and giardia lamblia stiles are enriched with filter device in water | |
| CN205042500U (en) | Cell centrifuging tube | |
| CN208649176U (en) | It is a kind of for extracting the separating pipe of high concentration growth factor | |
| CN110396058A (en) | A kind of novel calcifediol (25-hydroxyvitamin D3) isolation and purification method | |
| CN108892707A (en) | A kind of antibody purification device | |
| CN106967693B (en) | Cell culture virus liquid separation and purification method | |
| CN206809485U (en) | Filter cartridge is vented sterile protecting apparatus | |
| CN209065803U (en) | A kind of antibody purification device | |
| CN205839002U (en) | cell filter | |
| CN209178359U (en) | A kind of plants essential oil purification purifying plant | |
| CN203075375U (en) | Air exhausting device and filter of medical infusion device | |
| CN103484354A (en) | Nucleic acid extraction chip capable of extracting nucleic acid of gram-positive bacteria and gram-negative bacteria | |
| CN205473754U (en) | Cell filter | |
| CN107412279A (en) | A kind of extracting method of Cordceps militaris | |
| CN208266150U (en) | A kind of health wine treatment machine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant |