CN208617837U - A kind of enriching apparatus recycling dissociative DNA - Google Patents
A kind of enriching apparatus recycling dissociative DNA Download PDFInfo
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- CN208617837U CN208617837U CN201820977070.6U CN201820977070U CN208617837U CN 208617837 U CN208617837 U CN 208617837U CN 201820977070 U CN201820977070 U CN 201820977070U CN 208617837 U CN208617837 U CN 208617837U
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- circular cone
- straight tube
- dissociative dna
- tube
- toroidal cavity
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Abstract
The utility model discloses a kind of enriching apparatus for recycling dissociative DNA, including a probe, multiple magnetic beads, connecting tube, probe includes straight tube, circular cone, multiple communicating pipes, straight tube is the hollow cylinder of both ends open, toroidal cavity is formed in straight tube, multiple filter openings are uniformly provided on straight tube tube wall, filter opening one end is connected with the external world, the other end is connected with toroidal cavity；It is hollow structure inside circular cone, the coaxial straight tube of circular cone is arranged, and circular cone is connected with toroidal cavity, and the diameter of circular cone increases along the direction close to straight tube；Communicating pipe one end be connected with straight tube, the other end passes through circular cone outer wall, and multiple communicating pipes are circumferentially uniformly distributed along circular cone；Multiple magnetic beads are uniformly built in toroidal cavity；Connecting tube one end is connected with circular cone.Will be emerging intravenous in probe and magnetic bead merging, directly dissociative DNA is enriched in vein, and without extracting blood out, avoids the influence that cell rupture after blood extraction is enriched with dissociative DNA.
Description
Technical field
The utility model relates to biological sample separation technology fields, and in particular to a kind of enriching apparatus for recycling dissociative DNA.
Background technique
In healthy human body, there is a small amount of dissociative DNA, the free DNA in this part detects the initial stage of some diseases
It plays an important role, the method for dissociative DNA is first to extract blood from human body out in separated plasma at present, in blood after allowing
Dissociative DNA separated, free DNA is separated in the blood then extracted out, due to blood extract amount it is limited, from extraction blood
The amount for the dissociative DNA that can be isolated in liquid is less, and the blood extracted out has some apoptosis, the cell of apoptosis
DNA has part that can escape, and generates many interference to subsequent detection.
Utility model content
The purpose of the utility model is to overcome above-mentioned technical deficiencies, provide a kind of enriching apparatus for recycling dissociative DNA, solution
The technical issues of DNA to dissociate in blood is unfavorable for late detection is certainly separated by way of blood drawing in the prior art.
To reach above-mentioned technical purpose, the technical solution of the utility model provides a kind of enriching apparatus for recycling dissociative DNA,
Including a probe, multiple magnetic beads, connecting tube, the probe includes straight tube, circular cone, multiple communicating pipes, and the straight tube is both ends
The hollow cylinder of opening is formed with toroidal cavity in the straight tube, multiple filter openings is uniformly provided on the straight tube tube wall, described
Filter opening one end is connected with the external world, the other end is connected with the toroidal cavity；It is hollow structure inside the circular cone, it is described
The circular cone coaxially straight tube setting, and the circular cone is connected with the toroidal cavity, the diameter edge of the circular cone is leaned on
The direction of the nearly straight tube increases；Described communicating pipe one end is connected with the straight tube, the other end passes through the circular cone outer wall,
And multiple communicating pipes are circumferentially uniformly distributed along the circular cone；Multiple magnetic beads are uniformly built in the toroidal cavity；
Described connecting tube one end is connected with the circular cone.
Compared with prior art, the beneficial effects of the utility model include: will be emerging intravenous in probe and magnetic bead merging, directly
It connects and dissociative DNA is enriched in vein, the amount for the dissociative DNA that can be collected is more, and without extracting blood out, avoids blood
The influence that cell rupture is enriched with dissociative DNA after extraction；By setting circular cone, communicating pipe, make blood when flowing through probe, one
Part blood is flowed through from entering communicating pipe between straight tube, another part straight tube outer wall and blood vessel, is flowed to vessel inner blood
Hinder it is small, prevent straight tube block blood vessel；By the way that toroidal cavity is arranged in straight tube, magnetic bead is built in toroidal cavity, blood from
It is flowed through between the inner wall of straight tube and the outer wall of straight tube, increases the contact area of blood and straight tube, be conducive to dissociative DNA and enter ring
Shape cavity.
Detailed description of the invention
Fig. 1 is the schematic three dimensional views of the enriching apparatus of the utility model circulation dissociative DNA；
Fig. 2 is the structural schematic diagram of the enriching apparatus of the utility model circulation dissociative DNA；
Fig. 3 is the cross-sectional view of A-A line along Fig. 2；
Fig. 4 is the partial enlargement diagram in Fig. 3 at M；
Fig. 5 is the partial enlargement diagram in Fig. 3 at N.
Specific embodiment
In order to make the purpose of the utility model, technical solutions and advantages more clearly understood, below in conjunction with attached drawing and implementation
Example, the present invention will be further described in detail.It should be appreciated that specific embodiment described herein is only used to explain
The utility model is not used to limit the utility model.
The utility model provides a kind of enriching apparatus for recycling dissociative DNA, as shown in Fig. 1 to 5, including it is a probe 1, more
A magnetic bead 2, connecting tube 3, rubber stopper 4, probe 1 include straight tube 11, circular cone 12, multiple communicating pipes 13, and straight tube 11 is opened for both ends
The hollow cylinder of mouth, 11 outer diameter of straight tube are 4mm, and toroidal cavity is formed in straight tube 11, and the coaxial straight tube 11 of toroidal cavity is arranged, directly
It is uniformly provided with multiple filter openings on 11 tube wall of pipe, described filter opening one end is connected with the external world, the other end and the toroidal cavity phase
Connection, the filter opening diameter is preferably 0.6 μm.
Circular cone 12 is in truncated cone-shaped, is hollow structure, the setting of the coaxial straight tube 11 of circular cone 12, and taper inside circular cone 12
Block 12 is connected with the toroidal cavity, and the diameter of circular cone 12 increases along the direction close to straight tube 11；Communicating pipe 13 one end with
Straight tube 11 is connected, the other end passes through 12 outer wall of circular cone, and multiple communicating pipes 13 are circumferentially uniformly distributed along circular cone 12.
Multiple magnetic beads 2 are uniformly built in the toroidal cavity, and magnetic bead 2 is superparamagnetism silica nanometer magnetic bead 2, magnetic bead 2
Diameter be greater than the aperture of the filter opening, magnetic bead 2 can specifically identify on micro interface with nucleic acid molecules and efficient combination,
This magnetic bead 2 is commercially available.
3 one end of connecting tube is connected with circular cone 12, and connecting tube 3 is less than or equal to the one end of circular cone 12 far from straight tube 11
Diameter, 3 diameter of connecting tube is preferably 2.5mm.
4 part of rubber stopper is built in 3 other end of connecting tube, and 4 outer peripheral surface of rubber stopper is connected to 3 inner wall of connecting tube, and rubber
Fill in the other end setting of 4 coaxially connected pipes 3.
The specific workflow of the utility model: by syringe or other means, be probe 1, in connecting tube 3 full of life
Salt water is managed, by probe 1 through periphery venipuncture to central vein catheter, the dissociative DNA in blood enters toroidal cavity with blood plasma,
And other cell components (such as red blood cell, leukaemia, blood platelet etc.) in blood not can enter since size is larger, core at this time
Sour extraction specifically binds dissociative DNA with magnetic bead 2, and the dissociative DNA combined is trapped in toroidal cavity, is formed rich
Collection；When blood flows through straight tube 11 and circular cone 12, since circular cone 12 increases along the orient diameter close to straight tube 11, a part of blood
Liquid enters straight tube 11 from communicating pipe 13, and 11 inner wall of straight tube also offers filter hole, and dissociative DNA filter hole can enter ring from there
Shape cavity, another part blood are flowed through between 11 outer wall of straight tube and blood vessel, if direction of flow of blood and above-mentioned flowing side
To on the contrary, then a part of blood is flowed through between 11 outer wall of straight tube and blood vessel, another part blood is from straight tube 11 through being connected to
Pipe 13 passes through circular cone 12, and probe 1 is small to the obstruction of blood flow；After probe 1 is placed in 60 hours, probe 1 is taken out and carried out
Next step dissociative DNA analyzes and identifies work.
The beneficial effects of the utility model include, will be emerging intravenous in probe 1 and the merging of magnetic bead 2, right directly in vein
Dissociative DNA is enriched with, and the amount for the dissociative DNA that can be collected is more, and without extracting blood out, cell is broken after avoiding blood extraction
Split the influence to dissociative DNA enrichment；By the way that circular cone 12, communicating pipe 13 is arranged, make blood when flowing through probe 1, a part of blood
Liquid enters between straight tube 11,11 outer wall of another part straight tube and blood vessel from communicating pipe 13 to be flowed through, and is flowed to vessel inner blood
Hinder it is small, prevent straight tube 11 block blood vessel；By the way that toroidal cavity is arranged in straight tube 11, magnetic bead 2 is built in toroidal cavity, blood
Liquid flows through between the inner wall of straight tube 11 and the outer wall of straight tube 11, increases the contact area of blood Yu straight tube 11, is conducive to swim
Enter toroidal cavity from DNA；By the way that rubber stopper 4 is arranged, make probe 1, connecting tube 3 it is necessary to when can be used as PICC conduit and make
With.
Specific embodiment of the present utility model described above does not constitute the restriction to scope of protection of the utility model.
Any other various changes and modifications that any technical concept according to the present utility model is made should be included in practical
In novel scope of protection of the claims.
Claims (5)
1. a kind of enriching apparatus for recycling dissociative DNA, including a probe, multiple magnetic beads, connecting tube, the probe include straight tube,
Circular cone, multiple communicating pipes, the straight tube are the hollow cylinder of both ends open, are formed with toroidal cavity in the straight tube, described
It is uniformly provided with multiple filter openings on straight tube tube wall, described filter opening one end is connected with the external world, the other end and the toroidal cavity phase
Connection；It is hollow structure, the circular cone coaxially straight tube setting, and the circular cone and the ring inside the circular cone
Shape cavity is connected, and the diameter of the circular cone increases along the direction close to the straight tube；Described communicating pipe one end and described straight
Pipe is connected, the other end passes through the circular cone outer wall, and multiple communicating pipes are circumferentially uniformly distributed along the circular cone；It is more
A magnetic bead is uniformly built in the toroidal cavity；Described connecting tube one end is connected with the circular cone.
2. the enriching apparatus of circulation dissociative DNA according to claim 1, which is characterized in that the magnetic bead is superparamagnetism
Silica nanometer magnetic bead.
3. the enriching apparatus of circulation dissociative DNA according to claim 1, which is characterized in that the aperture of the filter opening is less than
The diameter of the magnetic bead.
4. the enriching apparatus of circulation dissociative DNA according to claim 1, which is characterized in that the richness of the circulation dissociative DNA
Acquisition means further include a rubber stopper, and the rubber plug portion is built in the connecting tube other end, and the rubber stopper outer peripheral surface supports
It is connected to the connection inside pipe wall, and the rubber stopper coaxially connecting tube other end setting.
5. the enriching apparatus of circulation dissociative DNA according to claim 1, which is characterized in that the circular cone is in truncated cone-shaped,
The connection pipe diameter is less than or equal to the diameter of the one end of the circular cone far from the straight tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820977070.6U CN208617837U (en) | 2018-06-25 | 2018-06-25 | A kind of enriching apparatus recycling dissociative DNA |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820977070.6U CN208617837U (en) | 2018-06-25 | 2018-06-25 | A kind of enriching apparatus recycling dissociative DNA |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208617837U true CN208617837U (en) | 2019-03-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820977070.6U Expired - Fee Related CN208617837U (en) | 2018-06-25 | 2018-06-25 | A kind of enriching apparatus recycling dissociative DNA |
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| CN (1) | CN208617837U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111471577A (en) * | 2020-05-22 | 2020-07-31 | 杭州同创医学检验实验室有限公司 | Gene dissociation DNA extraction system |
-
2018
- 2018-06-25 CN CN201820977070.6U patent/CN208617837U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111471577A (en) * | 2020-05-22 | 2020-07-31 | 杭州同创医学检验实验室有限公司 | Gene dissociation DNA extraction system |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190319 Termination date: 20210625 |