CN205258465U - Sperm separator - Google Patents
Sperm separator Download PDFInfo
- Publication number
- CN205258465U CN205258465U CN201520879919.2U CN201520879919U CN205258465U CN 205258465 U CN205258465 U CN 205258465U CN 201520879919 U CN201520879919 U CN 201520879919U CN 205258465 U CN205258465 U CN 205258465U
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- Prior art keywords
- sperm
- density gradient
- centrifugal
- inner tube
- tube
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Abstract
The utility model provides a sperm separator, includes the centrifuging tube, centrifuging tube internally mounted centrifugation inner tube and the interior pipe holder of centrifugation, and the pipe holder lies in beta % density gradient solution below with the cooperation of centrifugal inner tube, centrifuging tube inside splendid attire alpha % density gradient solution and beta % density gradient solution, alpha % density gradient solution in the centrifugation. It provides a sperm separator on density gradient centrifugation method basis, be equipped with the passageway centrifugation inner tube of special absorption separation sperm, avoid using during ordinary centrifuging tube carries out conventional density gradient centrifugation method operation process, upper impurity can some adhesions at centrifuging tube lateral wall and the following current liquid level to high density solution, and then the adhesion is in sneaking into the movable sperm of isolating the bottom to thoroughly separate refining and sperm. Avoided the not thorough problem of edulcoration, success rate, the rate of recovery and sperm quality are all good.
Description
Technical field
The utility model relates to a kind of cell biology field, specifically a kind of sperm separator.
Background technology
Sperm separating treatment technology is requisite key technology in auxiliary procreation technology, and while implementing auxiliary procreation technology, conventional sperm isolation technics mainly comprises density gradient centrifugation and upstream partition method. these technology all have limitation separately. the sperm rate of recovery of density gradient centrifugation is higher, but after finishing dealing with, need remove upper strata impurity and low-density solution with suction pipe, draw centrifuge tube bottom sperm with suction pipe by higher density solution liquid level again, like this, upper strata impurity can partly stick to centrifugal tube wall the following current liquid level to higher density solution, and then stick to and on suction pipe, sneak in the isolated motile sperm in bottom, therefore part separated upper strata Necrospermia and refining impurity are sneaked into bottom screening sperm by this operation meeting, although thereby the rate of recovery is high, but it is low compared with upper reaches method to reclaim sperm motility, particularly due to this limitation, under the special circumstances (as some refining infects virus or bacterium) that refining thoroughly need to be separated with sperm at some, just cannot the harmful substance existing in refining thoroughly be removed by the method, although upper reaches method can be avoided the situation of refining contaminating impurity sperm, the rate of recovery is generally lower. therefore prior art there is no method a kind of separation method of taking into account the rate of recovery and sperm quality is provided.
Utility model content
The purpose of this utility model is to provide a kind of sperm separator, and its rate of recovery is high and can effectively avoid refining impurity matter to pollute sperm, thereby can address the above problem.
The utility model for achieving the above object, be achieved through the following technical solutions: a kind of sperm separator, comprise centrifuge tube, inner centrifugal inner tube and the centrifugal interior pipe holder installed of centrifuge tube, centrifugal interior pipe holder coordinates with centrifugal inner tube, the inner splendid attire α % density gradient solution of centrifuge tube and β % density gradient solution, α % density gradient solution is positioned at β % density gradient solution below.
In order further to realize the purpose of this utility model, can also be by the following technical solutions: seal spiral cover is installed on described centrifuge tube top. In described centrifuge tube, be provided with convex annular edge, centrifugal interior pipe holder periphery is provided with sleeve pipe, sleeve pipe with convex annular along coordinating, centrifugal inner tube mid-stent is provided with swivel nut, centrifugal inner tube is provided with external screw thread, swivel nut is connected with centrifugal Internal Pipe Thread, and seal spiral cover bottom is provided with positioning sleeve, and positioning sleeve coordinates with centrifugal inner tube top. Described centrifugal interior pipe holder is provided with locating convex block, and convex annular is offered locating slot on edge, and locating slot coordinates with locating convex block. The bottom of described centrifuge tube is taper.
The utility model has the advantage of: it is that a kind of sperm separator is provided on density－gradient centrifuga－tion method basis, be provided with the centrifugal inner tube of passage (1) of special absorption separated sperm, avoid using common centrifuge tube to carry out in the operating process of conventional density gradient centrifugation, upper strata impurity can partly stick to centrifuge tube (2) sidewall the following current liquid level to higher density solution, and then stick to sneak into bottom isolated motile sperm in, thereby separate thorough to refining and sperm. Avoided the halfway problem of removal of impurities, success rate, the rate of recovery and sperm quality are all good.
By described centrifugal inner tube (1) and centrifugal interior pipe holder (4), in conjunction with the centrifugally operated that carries out certain rotating speed, sperm yield is improved greatly, be conducive to carry out follow-up seminal fluid separating treatment.
By being inserted to glass pipette (3) sucking-off, described centrifugal inner tube (1) reclaims the spermatium separating, therefore with in the process of suction pipe isolated at suction sperm, upper strata impurity being brought into the defect of separated sperm, refining material is thoroughly separated with sperm while having avoided making conventional common centrifuge tube separated sperm. The utility model also has advantages of compactness simple for structure, cheap for manufacturing cost and easy to use.
Brief description of the drawings
Fig. 1 is the use state of a kind of sperm separator described in the utility model; Fig. 2 is structural representation of the present utility model; Fig. 3 is the structural representation of the utility model another program; Fig. 4 is the A-A profile of Fig. 3.
Brief description of the drawings: centrifugal interior pipe holder 5 α % density gradient solution 6 β % density gradient solution 7 seal spiral cover 8 convex annular of 1 centrifugal inner tube 2 centrifuge tube 3 glass pipette 4 are along 9 positioning sleeve 10 sleeve pipe 11 swivel nut 12 locating slot 13 locating convex blocks.
Detailed description of the invention
A kind of sperm separator described in the utility model, as shown in Figure 2, comprises centrifuge tube 2, inner centrifugal inner tube 1 and the centrifugal interior pipe holder 4 installed of centrifuge tube 2. While extracting sperm, in centrifugal inner tube 1, can insert glass pipette 3, glass pipette 3 edges, centrifugal inner tube 1 directly extends centrifuge tube 2 bottoms. Centrifugal interior pipe holder 4 coordinates with centrifugal inner tube 1, the inner splendid attire α of centrifuge tube 2 % density gradient solution 5 and β % density gradient solution 6, and α % density gradient solution 5 is positioned at β % density gradient solution 6 belows. The bottom of described centrifuge tube 2 is taper. Seal spiral cover 7 is installed on described centrifuge tube 2 tops. 1.5 millimeters of the internal diameters that what described centrifugal inner tube 1 was upper and lower opening can allow glass pipette 3 or plastic suction pipe passes through, the tubular structure of 2 millimeters of external diameters. Described centrifuge tube 2 for specification be the centrifuge tube of 10-15ml. Described glass pipette 3 can be house steward and is about 23 centimetres, and tubule is about the aseptic clean glass pipette of 10 centimetres.
The purpose of this utility model is to provide a kind of sperm separator on density－gradient centrifuga－tion method basis, be provided with the centrifugal inner tube 1 of passage of special absorption separated sperm, avoid using common centrifuge tube to carry out in the operating process of conventional density gradient centrifugation, upper strata impurity can partly stick to centrifuge tube 2 sidewalls the following current liquid level to higher density solution, and then stick to sneak into bottom isolated motile sperm in, thereby separate thorough to refining and sperm. Avoided the halfway problem of removal of impurities, success rate, the rate of recovery and sperm quality are all good.
By described centrifugal inner tube 1 and centrifugal interior pipe holder 4, in conjunction with the centrifugally operated that carries out certain rotating speed, sperm yield is improved greatly, be conducive to carry out follow-up seminal fluid separating treatment.
Reclaim by described centrifugal inner tube 1 being inserted to glass pipette 3 sucking-offs the spermatium separating, therefore with in the process of suction pipe isolated at suction sperm, upper strata impurity being brought into the defect of separated sperm, refining material is thoroughly separated with sperm while having avoided making conventional common centrifuge tube separated sperm.
A kind of sperm partition method of utilizing a kind of sperm separator to implement, comprises the following steps:
(a) by sperm washing lotion, density gradient solution dilution is made into α % density gradient solution 5 and β % density gradient solution 6, the numerical value of described α is greater than the numerical value of β, the α % density gradient solution 5 of equivalent and β % density gradient solution 6 are applied to glass pipette 3 and divide and be placed on centrifuge tube bottom by centrifugal inner tube, and make described α % density gradient solution be positioned at the below of β % density gradient solution and interface is clear between the two;
(b) arrive through centrifugal interior pipe holder 4 the density gradient solution top that step a does with glass pipette 3, seminal fluid is slowly dripped on β % density gradient solution 6 tops, note not destroying the complete of liquid level;
(c) closing seam screw lid 7 is by the ferrule of centrifuge tube 2;
(f) centrifuge tube 2 and centrifugal inner tube 1 were carried out to low-speed centrifugal processing after 15 ~ 20 minutes, arrive centrifuge tube bottom by inner tube with a clean aseptic long bus moral suction pipe, by the spermatium sucking-off of bottom, move and in other clean centrifuge tube, carry out follow-up Sperm washing processing.
As shown in Figure 3, in described centrifuge tube 2, be provided with convex annular along 8, centrifugal interior pipe holder 4 peripheries are provided with sleeve pipe 10, and sleeve pipe 10 coordinates along 8 with convex annular. Centrifugal interior pipe holder 4 middle parts are provided with swivel nut 11, and centrifugal inner tube 1 is provided with external screw thread, and swivel nut 11 is threaded with centrifugal inner tube 1, and swivel nut 11 and centrifugal inner tube 1 are connected firmly, and are convenient to processing assembling. Seal spiral cover 7 bottoms are provided with positioning sleeve 9, and positioning sleeve 9 coordinates with centrifugal inner tube 1 top, to centrifugal inner tube 1 is located, prevent that centrifugal inner tube 1 from rocking.
For preventing that sleeve pipe 10 from rotating along 8 relative to convex annular, as shown in Figure 4, described centrifugal interior pipe holder 4 is provided with locating convex block 13, and convex annular is along offering locating slot 12 on 8, and locating slot 12 coordinates with locating convex block 13.
The technical solution of the utility model is not restricted in the scope of embodiment described in the utility model. The utility model not technology contents of detailed description is known technology.
Claims (5)
1. a sperm separator, it is characterized in that: comprise centrifuge tube (2), inner centrifugal inner tube (1) and the centrifugal interior pipe holder (4) installed of centrifuge tube (2), centrifugal interior pipe holder (4) coordinates with centrifugal inner tube (1), centrifuge tube (2) inner splendid attire α % density gradient solution (5) and β % density gradient solution (6), α % density gradient solution (5) is positioned at β % density gradient solution (6) below.
2. a kind of sperm separator according to claim 1, is characterized in that: seal spiral cover (7) is installed on described centrifuge tube (2) top.
3. a kind of sperm separator according to claim 2, it is characterized in that: in described centrifuge tube (2), be provided with convex annular along (8), centrifugal interior pipe holder (4) periphery is provided with sleeve pipe (10), sleeve pipe (10) coordinates along (8) with convex annular, centrifugal interior pipe holder (4) middle part is provided with swivel nut (11), centrifugal inner tube (1) is provided with external screw thread, swivel nut (11) is threaded with centrifugal inner tube (1), seal spiral cover (7) bottom is provided with positioning sleeve (9), positioning sleeve (9) coordinates with centrifugal inner tube (1) top.
4. a kind of sperm separator according to claim 3, it is characterized in that: described centrifugal interior pipe holder (4) is provided with locating convex block (13), convex annular is along offering locating slot (12) on (8), and locating slot (12) coordinates with locating convex block (13).
5. a kind of sperm separator according to claim 1, is characterized in that: the bottom of described centrifuge tube (2) is taper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520879919.2U CN205258465U (en) | 2015-11-06 | 2015-11-06 | Sperm separator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520879919.2U CN205258465U (en) | 2015-11-06 | 2015-11-06 | Sperm separator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205258465U true CN205258465U (en) | 2016-05-25 |
Family
ID=56000003
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520879919.2U Expired - Fee Related CN205258465U (en) | 2015-11-06 | 2015-11-06 | Sperm separator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205258465U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107008518A (en) * | 2017-04-12 | 2017-08-04 | 郝大勇 | Centrifuge tube, centrifuge tube adapter and the method for extracting sample |
-
2015
- 2015-11-06 CN CN201520879919.2U patent/CN205258465U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107008518A (en) * | 2017-04-12 | 2017-08-04 | 郝大勇 | Centrifuge tube, centrifuge tube adapter and the method for extracting sample |
| CN107008518B (en) * | 2017-04-12 | 2019-08-09 | 郝大勇 | Centrifuge tube, centrifuge tube adapter and the method for extracting sample |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160525 Termination date: 20161106 |