CN107008518A - Centrifuge tube, centrifuge tube adapter and the method for extracting sample - Google Patents

Centrifuge tube, centrifuge tube adapter and the method for extracting sample Download PDF

Info

Publication number
CN107008518A
CN107008518A CN201710235804.3A CN201710235804A CN107008518A CN 107008518 A CN107008518 A CN 107008518A CN 201710235804 A CN201710235804 A CN 201710235804A CN 107008518 A CN107008518 A CN 107008518A
Authority
CN
China
Prior art keywords
centrifuge tube
centrifugation
lid
sample
suction pipe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201710235804.3A
Other languages
Chinese (zh)
Other versions
CN107008518B (en
Inventor
郝大勇
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Mingyue Medical Technology Co.,Ltd.
Original Assignee
郝大勇
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 郝大勇 filed Critical 郝大勇
Priority to CN201710235804.3A priority Critical patent/CN107008518B/en
Publication of CN107008518A publication Critical patent/CN107008518A/en
Application granted granted Critical
Publication of CN107008518B publication Critical patent/CN107008518B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021Test tubes specially adapted for centrifugation purposes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/24Apparatus for enzymology or microbiology tube or bottle type
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • G01N1/10Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14Suction devices, e.g. pumps; Ejector devices

Abstract

Method the present invention relates to centrifuge tube, centrifuge tube adapter and for extracting sample.The centrifuge tube adapter is used to coordinate centrifugation tube body using to extract the sample after centrifugation positioned at the center section of centrifuge tube body interior or bottom, and it includes:Centrifuge tube lid, it is used for the sample for closing centrifuge tube body interior;Centrifuge tube transition lid, centrifuge tube lid can be installed on centrifuge tube transition lid, and centrifuge tube transition cover body can be installed on centrifugation tube body;Suction pipe, it is fixed on centrifuge tube transition lid and for extracting the sample after centrifugation positioned at the center section of the centrifuge tube body interior or bottom.The centrifuge tube and its centrifuge tube adapter of the present invention, can be located at the sample for centrifuging tube body center section or bottom after the decomposition of extraction and application density-gradient centrifugation method, and the sample extracted is not polluted by upper sample or other mixed liquors.

Description

Centrifuge tube, centrifuge tube adapter and the method for extracting sample
Technical field
The present invention relates to medical instruments field, more particularly to a kind of centrifuge tube and centrifuge tube adapter and for extracting The method of sample.
Background technology
Density-gradient centrifugation method refers to form continuous or discrete density gradient in centrifuge tube with certain medium, incited somebody to action Cell suspension or homogenate are placed in the top of medium, cell is layered, separated by the effect of gravity or centrifugal force field.It is close at present Degree gradient centrifugation is widely used in seperated nuclear acid, subcellular organelle, compound protein and other compositions.Utilizing density gradient Centrifugal process separation cell after, under extraction during confluent monolayer cells, often influenceed by upper component, and can not extract it is pure under Confluent monolayer cells.
For example, in auxiliary procreation technology, the optimization processing for semen is a conventional application technology.Using close Spend gradient centrifugation and carry out seminal fluid optimization processing, be not only able to filter out form and the good sperm of vigor for helping pregnant treatment, and There is the effect of certain removal microorganism for the seminal fluid of inflammation and patients with infectious diseases.But in the prior art, using normal Advise centrifuge tube and density gradient centrifugation is carried out to seminal fluid, the seminal fluid after lower floor's optimization is extracted, generally using two methods:One kind is Upper strata refining, gradient liquid are drawn successively to abandon, and then draw the seminal fluid after required optimization;Another is directly to use suction pipe Lower floor's seminal fluid is drawn through upper strata refining and gradient liquid.
All there is the risk that upper strata refining is mixed into the seminal fluid after lower floor's optimization in above two method.Semen sample is state Potential infection source of family's regulation with " two grades of bio-safety " level, the infection risk of semen sample and male's seminal vesicle inflammation and its The communicable virus carried is closely related.Therefore, using density gradient centrifugation method separation seminal fluid after, how to obtain not by The optimization seminal fluid of upper strata refining pollution turns into our urgent problems to be solved at present.
The content of the invention
For technical problem present in prior art, the present invention provides a kind of centrifuge tube and centrifuge tube adapter and use In the method for extracting sample.It can be extracted by the present invention and be located at centrifugation tube body center section after density-gradient centrifugation method is decomposed Or the sample of bottom, and the sample extracted do not polluted by upper sample or other mixed liquors.
The present invention provides a kind of centrifuge tube adapter, and it is used to coordinate centrifugation tube body to use to extract by centrifugation Afterwards positioned at the sample of the center section of centrifuge tube body interior or bottom, the centrifuge tube adapter includes:Centrifuge tube lid, its The sample for closing the centrifuge tube body interior;Centrifuge tube transition lid, the centrifuge tube lid can be installed on The centrifuge tube transition lid, the centrifuge tube transition cover body can be installed on the centrifugation tube body;Suction pipe, it is fixed on institute State centrifuge tube transition lid and for extract after centrifugation positioned at the centrifuge tube body interior center section or The sample of bottom.
Preferably, the suction pipe extends to the bottom of the centrifuge tube body interior, and for extracting the centrifuge tube The sample of the bottom of body interior.
Preferably, the suction pipe extends to the center section of the centrifuge tube body interior, and for extract it is described from The sample of center section inside heart pipe.
Preferably, the internal diameter of the suction pipe is more than or equal to 0.25cm and less than or equal to 0.5cm.
Preferably, the centrifuge tube transition lid is provided with for the sample to be added into one of the centrifugation tube body Or multiple through holes.
Preferably, the centrifuge tube lid is connected through a screw thread or is interference fitted with the centrifuge tube transition lid and is connected; And/or the centrifuge tube transition lid is connected through a screw thread or is interference fitted with the centrifugation tube body and is connected.
Preferably, the suction pipe is higher by the upper surface of the centrifuge tube transition lid.
The present invention also provides a kind of centrifuge tube, and the centrifuge tube includes:Tube body is centrifuged, it is used to contain and centrifuged Sample;And according to the centrifuge tube adapter of the present invention, it can be connected with the centrifugation tube body, for from the centrifuge tube Body interior extracts the sample.
Preferably, the centrifugation tube body is conical centrifuge tube body.
The present invention also provides a kind of method for extracting sample, and this method is used to be extracted according to the centrifuge tube of the present invention and passed through The sample of the center section or bottom that are located at centrifuge tube body interior is crossed after centrifuging, be the described method comprises the following steps:
Step A, is installed on the centrifugation tube body, by being formed at by the centrifuge tube transition lid for being fixed with suction pipe The through hole of the centrifuge tube transition lid sequentially adds density gradient liquid into the centrifugation tube body and treats spun specimen,
Or, the centrifuge tube transition lid for being fixed with the suction pipe is first inserted into the centrifugation tube body, and incline The oblique centrifuge tube transition lid, sequentially adds density gradient liquid at the opening of the centrifugation tube body and treats spun specimen, Then the centrifuge tube transition lid is installed on the centrifugation tube body;
Step B, the centrifuge tube transition lid is installed on by the centrifuge tube lid;
Step C, centrifuge is put into by the centrifuge tube, and the predetermined time is centrifuged under predetermined centrifugal force;
Step D, opens the centrifuge tube lid, and diameter is less than to another suction pipe insertion suction of the diameter of the suction pipe In pipe, another suction pipe is set to stretch into the bottom of the suction pipe to extract sample.
The centrifuge tube and centrifuge tube adapter of the present invention, can extract after density-gradient centrifugation method is decomposed and be located at centrifuge tube sheet Body center section or the sample of bottom, and the sample extracted do not polluted by upper sample or other mixed liquors, and And the centrifugation adapter structure of the present invention is simple, highly versatile, and conventional centrifuge tube can be coordinated to use.
Brief description of the drawings
Fig. 1 is the structural representation of existing conventional centrifugal pipe;
Fig. 2 is the structural representation of the centrifuge tube according to the present invention;
Fig. 3 is the sectional view of the centrifuge tube according to the present invention;
Fig. 4 is the structural representation that centrifuge tube lid and centrifugation tube body are omitted according to the centrifuge tube of the present invention;
Fig. 5 is the top view of the centrifuge tube transition lid of the centrifuge tube of the invention according to Fig. 2;
Fig. 6 is the sectional view of the centrifuge tube adapter according to the present invention.
Description of reference numerals
100- centrifuge tube lids;200- centrifuge tube transition lids;210- external thread parts;220- internal thread parts;230- through holes; 300- suction pipes;400- centrifuges tube body.
Embodiment
Below with reference to the accompanying drawings the centrifuge tube and centrifuge tube adapter of the present invention are described in detail and illustrated.
Fig. 1 is the structural representation of existing conventional centrifugal pipe.Existing conventional centrifugal pipe by centrifuge tube lid 100 and from Heart tube body 400 is constituted, extraction and application density-gradient centrifugation method separation after sample when, if required sample be located at from During the bottom of heart tube body 400, suction pipe can only be inserted and centrifuge the bottom of tube body 400 and extract, or by the mixing on upper strata Liquid or other samples are removed, and can extract the sample needed for lower floor.But above two operating method can all cause lower floor The problem of sample is polluted by upper strata mixed liquor or other samples.
Fig. 2 is the structural representation of the centrifuge tube according to the present invention.Fig. 3 is the sectional view of the centrifuge tube according to the present invention. Referring to figs. 2 and 3, centrifuge tube of the invention include centrifuge tube lid 100, centrifuge tube transition lid 200, suction pipe 300 and from Heart tube body 400.As shown in figure 3, in present embodiment, suction pipe 300 is fixed on centrifuge tube transition lid 200, and suction pipe 300 Upper end be higher by the upper surface of centrifuge tube transition lid 200.It can so avoid adding sample and close to centrifugation tube body 400 Spend gradient liquid when, aliquot either density gradient liquid flow into suction pipe 300 in or the opening of suction pipe 300 at by sample Pollution.Centrifuge tube lid 100 is tightened in centrifuge tube transition lid 200, the upper end of suction pipe 300 and centrifuge tube lid by screw thread Gap is left between 100.
With reference to Fig. 4 and Fig. 5, centrifuge tube transition lid 200 have the external thread part 210 that is connected with centrifuge tube lid 100 and The internal thread part 220 being connected with centrifugation tube body 400.From figure 5 it can be seen that centrifuge tube transition lid 200 is provided with two Through hole 230 for adding sample and density gradient liquid.Sample and density gradient liquid can be added by centrifuge tube by through hole 230 Body 400.
As shown in figure 3, suction pipe 300 extends to the bottom of centrifugation tube body 400, therefore, can directly it be carried by suction pipe 300 Take the sample of centrifugation tube body 400 bottom, and the sample extracted will not by centrifuge tube top or middle part other examinations The pollution of sample or mixed liquor.Equally, suction pipe 300 can also extend to other any positions of centrifugation tube body 400, to extract Required sample.
The internal diameter of suction pipe 300 is preferably greater than or equal to 0.25cm and is less than or equal to 0.5cm.If in suction pipe 300 Footpath is too small, due to capillarity, and substantial amounts of gradient liquid can be sucked in suction pipe 300, so as to influence the extraction of sample;If suction pipe 300 internal diameters are too big, then are unfavorable for the control of sample extracted amount.
It is preferably taper tube body to centrifuge tube body 400.Because, if after centrifugation, the sample to be extracted This is located at the bottom of centrifuge tube body and content is seldom, and the base diameter of taper tube body is less than the cylindrical tube sheet of same specification The base diameter of body, the then liquid level for the sample to be extracted is higher relative to the liquid level of the cylindrical tube body of same specification, so It is easy to the extraction of sample.
In the present embodiment, preferably, passing through screw thread between centrifuge tube lid 100 and centrifuge tube transition lid 200 Connection, also by threaded connection between centrifuge tube transition lid 200 and centrifugation tube body 400.Threaded connection is solid and reliable, be easy to Dismounting, while can also avoid splashing of sample etc. in unloading process.
Fig. 6 is the sectional view of the centrifuge tube adapter of the present invention, and it includes centrifuge tube lid 100, centrifuge tube transition lid 200 and suction pipe 300.Suction pipe 300 is fixed on centrifuge tube transition lid 200, and the top of suction pipe 300 is higher by centrifugation tube transitions The upper surface of lid 200.Centrifuge tube lid 100 is tightened in centrifuge tube transition lid 200 by screw thread, the upper end of suction pipe 300 and Gap is left between centrifuge tube lid 100.
The external thread part 210 of centrifuge tube transition lid 200 in the centrifuge tube adapter of the present invention can be with routine The external screw thread for centrifuging tube body is identical, and the internal thread part 220 of centrifuge tube transition lid 200 can be with conventional centrifugal lid body Internal thread is identical, so, and centrifuge tube adapter of the invention can be directly applied for conventional centrifuge tube.
As the present invention preferred scheme, centrifuge tube lid 100, centrifuge tube transition lid 200 and centrifugation tube body 400 it Between be connected through a screw thread.It is understood that centrifuge tube lid 100, centrifuge tube transition lid 200 and centrifuge tube sheet in the present invention Plug-in connection or other connected modes that can also be by interference fit between body 400 be connected.
The application of centrifuge tube adapter of the invention in auxiliary procreation technology is described in detail and illustrated below.
Separated by density-gradient centrifugation method after seminal fluid, the sample seminal fluid after optimization is in the bottom of centrifuge tube.According to existing There is the method commonly used in technology, when extracting lower floor's sample seminal fluid, it is impossible to avoid upper strata refining to the sample seminal fluid to be extracted Pollution.The centrifuge tube of the present invention has the suction pipe 300 for extending to centrifugation tube body 400 bottom due to it, can be easily The sample seminal fluid after the optimization of bottom is extracted, while ensureing that extracted sample seminal fluid is not polluted by upper strata refining.
Specifically used process of the centrifuge tube in auxiliary procreation technology of the present invention be:By the centrifuge tube adapter of the present invention Centrifugation tube body 400 is installed on, centrifuge tube lid 100 is opened.Density gradient centrifugation liquid is passed through into centrifugation according to the height of concentration The through hole 230 of tube transitions lid 200 is sequentially added in centrifugation tube body 400.Then initial seminal fluid is proportionally passed through into centrifugation The through hole 230 of tube transitions lid 200 is added in centrifugation tube body 400.Now, into the only density gradient inside suction pipe 300 Liquid.Centrifuge tube lid 100 is tightened in centrifuge tube transition lid 200.Finally centrifuge tube is placed in centrifuge, in 300g centrifugations Under power, centrifugation 20 minutes is carried out.Centrifuge tube lid 100 is opened, diameter is less than to another suction pipe (not shown) of suction pipe 300 The bottom of suction pipe 300 is inserted, the sample seminal fluid after optimization is extracted from the bottom of suction pipe 300.It is of course also possible to directly use this hair Bright centrifuge tube carries out density-gradient centrifugation method separation to original seminal fluid.Because the lower end of suction pipe 300 is not in contact with original seminal fluid, from After heart separation, only it is located at the density gradient liquid on the top of suction pipe 300 and positioned at the bottom of suction pipe 300 into inside suction pipe 300 Sample seminal fluid after optimization, therefore the sample seminal fluid extracted will not be polluted by the smart oar in upper strata.
In above process, if centrifuge tube transition lid 200 is not provided with through hole 230, first suction pipe will can be fixed with The 300 insertion centrifugation tube body 400 of centrifuge tube transition lid 200, and centrifuge tube transition lid 200 is tilted, by density gradient Centrifugate adds centrifugation tube body 400 according to the height of concentration at the opening of centrifugation tube body 400, then in the same way Proportionally initial seminal fluid is added and is centrifuged in centrifugation tube body 400.
Likewise, the present invention centrifuge tube and centrifuge tube adapter, can be applied to other mixed liquor density gradient from In heart method isolation technics, if to extract the sample of centrifugation tube body 400 center section, it is only necessary to according to the actual requirements using suitable When the suction pipe 300 of length.
According to the embodiment of the present invention, two through holes are provided with centrifuge tube transition lid 200, but should manage The centrifuge tube transition lid 200 for the present invention is solved it can also be provided that other structures, such as engraved structure, if guarantee by Suction pipe 300 is fixed on centrifuge tube transition lid 200, and one or more through holes with addition sample and density gradient liquid are Can.
The present invention can be applied equally to nucleic acid, subcellular organelle in addition to it can be applied to above-mentioned auxiliary procreation technology And the extraction of the sample that can be separated by density-gradient centrifugation method of compound protein etc..
The centrifuge tube adapter of the present invention can coordinate conventional centrifuge tube to use, highly versatile, simple in construction, user Just.The process density-gradient centrifugation method point in centrifuge tube can be extracted by using the centrifuge tube or centrifuge tube adapter of the present invention From it is rear be located at centrifugation tube body center section or bottom sample, and the sample extracted will not by upper strata or other The pollution of the sample or mixed liquor of position.
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, any Those familiar with the art disclosed herein technical scope in, the change or replacement that can be readily occurred in, all It should be included within the scope of the present invention.

Claims (10)

1. a kind of centrifuge tube adapter, it is used to coordinate centrifugation tube body to be located at centrifuge tube after centrifugation using to extract The center section of body interior or the sample of bottom, it is characterised in that the centrifuge tube adapter includes:
Centrifuge tube lid, it is used for the sample for closing the centrifuge tube body interior;
Centrifuge tube transition lid, the centrifuge tube lid can be installed on the centrifuge tube transition lid, the centrifugation tube transitions Lid can be installed on the centrifugation tube body;
Suction pipe, it is fixed on the centrifuge tube transition lid and is located at the centrifuge tube sheet after centrifugation for extracting The center section in internal portion or the sample of bottom.
2. centrifuge tube adapter according to claim 1, it is characterised in that the suction pipe extends to the centrifugation tube body Internal bottom, and for the sample for the bottom for extracting the centrifuge tube body interior.
3. centrifuge tube adapter according to claim 1, it is characterised in that the suction pipe extends to the centrifugation tube body Internal center section, and for extracting the sample of the center section inside the centrifuge tube.
4. centrifuge tube adapter according to claim 1, it is characterised in that the internal diameter of the suction pipe is more than or equal to 0.25cm and less than or equal to 0.5cm.
5. centrifuge tube adapter according to claim 1, it is characterised in that the centrifuge tube transition lid, which is provided with, to be used for The sample is added to one or more through holes of the centrifugation tube body.
6. centrifuge tube adapter according to claim 1, it is characterised in that
The centrifuge tube lid is connected through a screw thread or is interference fitted with the centrifuge tube transition lid and is connected;And/or
The centrifuge tube transition lid is connected through a screw thread or is interference fitted with the centrifugation tube body and is connected.
7. centrifuge tube adapter according to claim 1, it is characterised in that the suction pipe is higher by the centrifuge tube transition cover The upper surface of body.
8. a kind of centrifuge tube, it is characterised in that the centrifuge tube includes:
Tube body is centrifuged, it is used to containing and centrifuging sample;
Centrifuge tube adapter according to any one of claim 1-7, it can be connected with the centrifugation tube body, be used for The sample is extracted from the centrifuge tube body interior.
9. centrifuge tube according to claim 8, it is characterised in that the centrifugation tube body is conical centrifuge tube body.
10. a kind of method for extracting sample, this method is extracted by centrifugation using centrifuge tube according to claim 8 Positioned at the sample of the center section of centrifuge tube body interior or bottom after separation, it the described method comprises the following steps:
Step A, the centrifugation tube body is installed on by the centrifuge tube transition lid for being fixed with suction pipe, described by being formed at The through hole of centrifuge tube transition lid sequentially adds density gradient liquid into the centrifugation tube body and treats spun specimen,
Or, the centrifuge tube transition lid for being fixed with the suction pipe is first inserted into the centrifugation tube body, and tilt institute Centrifuge tube transition lid is stated, density gradient liquid is sequentially added at the opening of the centrifugation tube body and treats spun specimen, then The centrifuge tube transition lid is installed on the centrifugation tube body;
Step B, the centrifuge tube transition lid is installed on by the centrifuge tube lid;
Step C, centrifuge is put into by the centrifuge tube, and the predetermined time is centrifuged under predetermined centrifugal force;
Step D, opens the centrifuge tube lid, and another suction pipe that diameter is less than to the diameter of the suction pipe inserts the suction pipe It is interior, make another suction pipe stretch into the bottom of the suction pipe to extract sample.
CN201710235804.3A 2017-04-12 2017-04-12 Centrifuge tube, centrifuge tube adapter and the method for extracting sample Active CN107008518B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710235804.3A CN107008518B (en) 2017-04-12 2017-04-12 Centrifuge tube, centrifuge tube adapter and the method for extracting sample

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710235804.3A CN107008518B (en) 2017-04-12 2017-04-12 Centrifuge tube, centrifuge tube adapter and the method for extracting sample

Publications (2)

Publication Number Publication Date
CN107008518A true CN107008518A (en) 2017-08-04
CN107008518B CN107008518B (en) 2019-08-09

Family

ID=59446415

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710235804.3A Active CN107008518B (en) 2017-04-12 2017-04-12 Centrifuge tube, centrifuge tube adapter and the method for extracting sample

Country Status (1)

Country Link
CN (1) CN107008518B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108786950A (en) * 2018-03-30 2018-11-13 江苏科华医疗器械科技有限公司 A kind of lift takes liquid centrifuge tube
CN112295629A (en) * 2019-08-02 2021-02-02 上海唯问生物技术有限公司 Centrifuge tube

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2198606A1 (en) * 1994-08-31 1996-03-07 Peter Van Vlasselaer Centrifuge syringe apparatus and method
JP2002116201A (en) * 2000-10-11 2002-04-19 Sekisui Chem Co Ltd Cell-function measuring container
CN2721094Y (en) * 2004-07-21 2005-08-31 张长青 Centrifugal tube for preparing blood cell enriched plasma
CN104651224A (en) * 2015-02-10 2015-05-27 张丽红 Double-tube vacuum film sperm separation method and separation device
CN204768798U (en) * 2015-06-30 2015-11-18 天津市康婷生物工程有限公司 Filterable suit centrifuging tube
CN205258465U (en) * 2015-11-06 2016-05-25 山东省计划生育科学技术研究所 Sperm separator

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2198606A1 (en) * 1994-08-31 1996-03-07 Peter Van Vlasselaer Centrifuge syringe apparatus and method
JP2002116201A (en) * 2000-10-11 2002-04-19 Sekisui Chem Co Ltd Cell-function measuring container
CN2721094Y (en) * 2004-07-21 2005-08-31 张长青 Centrifugal tube for preparing blood cell enriched plasma
CN104651224A (en) * 2015-02-10 2015-05-27 张丽红 Double-tube vacuum film sperm separation method and separation device
CN204768798U (en) * 2015-06-30 2015-11-18 天津市康婷生物工程有限公司 Filterable suit centrifuging tube
CN205258465U (en) * 2015-11-06 2016-05-25 山东省计划生育科学技术研究所 Sperm separator

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108786950A (en) * 2018-03-30 2018-11-13 江苏科华医疗器械科技有限公司 A kind of lift takes liquid centrifuge tube
CN108786950B (en) * 2018-03-30 2020-09-22 江苏科华医疗器械科技有限公司 Liquid centrifuging tube is got to over-and-under type
CN112295629A (en) * 2019-08-02 2021-02-02 上海唯问生物技术有限公司 Centrifuge tube

Also Published As

Publication number Publication date
CN107008518B (en) 2019-08-09

Similar Documents

Publication Publication Date Title
US20110117650A1 (en) Vessel and method for isolating cells from tissue portions
JP4398033B2 (en) Assembly for separating fluid sample components
US6409528B1 (en) Device and method for collecting, preparation and stabilizing a sample
EP2254991B1 (en) A system and process for separating a material
CN113751095B (en) Sample container and method for separating serum or plasma from whole blood
WO1998048938A1 (en) Semi-continuous, small volume centrifugal blood separator
CN107008518A (en) Centrifuge tube, centrifuge tube adapter and the method for extracting sample
ES2210724T3 (en) DEVICE FOR WASHING CELLS AND ASSOCIATED PROCEDURE.
JPS63267459A (en) Decanting cell for blood and biochemical medical fluid
US11344880B2 (en) Centrifuge tube separation system, and methods of use
US20230039229A1 (en) Biological material collection and separation system and methods of use
US20190025162A1 (en) Blood and Marrow Draw Processing Devices and Methods
JP5668075B2 (en) Stem cell isolation method
CN112263862A (en) Sample preservation liquid bottle, sample slide device and cell pretreatment method
CN206940863U (en) A kind of 24 hole treatments of the sample plates of automatic separation of solid and liquid
US20020164273A1 (en) Evacuated tube and method for microscopy examination of urine sediment, chemistry and microbiological assays
CN206121222U (en) Little extraction device of automatic dispersion liquid liquid of extraction tube and applied this pipe
CN214496302U (en) A centrifuging tube for peripheral blood draws DNA
CN207793276U (en) A kind of solid-liquid diversion reaction extraction system
CN215250799U (en) Device for extracting platelet-rich plasma endogenous exosome
CN209317122U (en) Tear separator
CN206127178U (en) Cell membrane albumen extracts kit with cytoplasm albumen
JP2006246835A (en) Separation/recovery container for density gradient
US10130951B2 (en) Open-ended conical tube for recovering cells from a microfludic chip
CN212632739U (en) PBMC centrifuge tube with magnetic handle

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20200909

Address after: 450052 No. 7 rehabilitation front street, Henan, Zhengzhou

Patentee after: THE THIRD AFFILIATED HOSPITAL OF ZHENGZHOU University

Address before: 450052 No. 7 rehabilitation front street, Henan, Zhengzhou

Patentee before: Hao Dayong

TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20201221

Address after: 201318 Room 401, building 24, no.1-28, Lane 588, Tianxiong Road, Pudong New Area, Shanghai

Patentee after: Shanghai Mingyue Medical Technology Co.,Ltd.

Address before: 450052 No. 7 rehabilitation front street, Henan, Zhengzhou

Patentee before: THE THIRD AFFILIATED HOSPITAL OF ZHENGZHOU University