CN107008518B - Centrifuge tube, centrifuge tube adapter and the method for extracting sample - Google Patents
Centrifuge tube, centrifuge tube adapter and the method for extracting sample Download PDFInfo
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- CN107008518B CN107008518B CN201710235804.3A CN201710235804A CN107008518B CN 107008518 B CN107008518 B CN 107008518B CN 201710235804 A CN201710235804 A CN 201710235804A CN 107008518 B CN107008518 B CN 107008518B
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- China
- Prior art keywords
- centrifuge tube
- lid
- sample
- centrifugation
- centrifuge
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/24—Apparatus for enzymology or microbiology tube or bottle type
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
Abstract
The present invention relates to centrifuge tube, centrifuge tube adapter and methods for extracting sample.The centrifuge tube adapter is used to that centrifugation tube body to be cooperated to use the sample to extract the middle section or bottom that are located at centrifuge tube body interior after centrifuge separation comprising: centrifuge tube lid is used to close the sample of centrifuge tube body interior；Centrifuge tube transition lid, centrifuge tube lid can be installed on centrifuge tube transition lid, and centrifuge tube transition cover body can be installed on centrifugation tube body；Suction pipe is fixed on centrifuge tube transition lid and the sample for extracting the middle section or bottom that are located at the centrifuge tube body interior after centrifuge separation.Centrifuge tube and its centrifuge tube adapter of the invention, can extraction and application density-gradient centrifugation method decompose after be located at the centrifugation tube body middle section perhaps sample of bottom and extracted sample is not by the pollution of upper sample or other mixed liquors.
Description
Technical field
The present invention relates to medical instruments fields, more particularly to a kind of centrifuge tube and centrifuge tube adapter and for extracting
The method of sample.
Background technique
Density-gradient centrifugation method refers to forms continuous or discontinuous density gradient with certain medium in centrifuge tube, will
Cell suspension or homogenate are placed in the top of medium, make cell layering, separation by the effect of gravity or centrifugal force field.It is close at present
Degree gradient centrifugation is widely used in separation nucleic acid, subcellular organelle, compound protein and other compositions.Utilizing density gradient
Centrifugal process separate cell after, under extraction when confluent monolayer cells, often influenced by upper component, and can not extract it is pure under
Confluent monolayer cells.
For example, the optimization processing for semen is a conventional application technology in assisted reproductive technology.Using close
It spends gradient centrifugation and carries out sperm optimization processing, be not only able to filter out form and the good sperm of vigor for helping pregnant treatment, and
Has the function of certain removal microorganism for the sperm of inflammation and patients with infectious diseases.But in the prior art, using normal
It advises centrifuge tube and density gradient centrifugation is carried out to sperm, the sperm after extracting lower layer's optimization generallys use two methods: one is
Successively draw upper layer refining, gradient liquid abandons, the sperm after optimization required for then drawing；Another kind is directly to use suction pipe
Lower layer's sperm is drawn across upper layer refining and gradient liquid.
All there is the risk that upper layer refining is mixed into the sperm after lower layer's optimization in above two method.Semen sample is state
Family's regulation has a Potential infection source of " bio-safety second level " level, the infection risk of semen sample and male's seminal vesicle inflammation and its
The communicable virus carried is closely related.Therefore, using density gradient centrifugation method separation sperm after, how to obtain not by
The optimization sperm of upper layer refining pollution becomes our urgent problems to be solved at present.
Summary of the invention
For the technical problems in the prior art, the present invention provides a kind of centrifuge tube and centrifuge tube adapter and use
In the method for extracting sample.It can extract through the invention and be located at centrifugation tube body middle section after density-gradient centrifugation method decomposes
Perhaps the sample of bottom and extracted sample is not by the pollution of upper sample or other mixed liquors.
The present invention provides a kind of centrifuge tube adapter, is used to that centrifugation tube body to be cooperated to use to extract by centrifuge separation
Afterwards positioned at the sample of the middle section of centrifuge tube body interior or bottom, the centrifuge tube adapter includes: centrifuge tube lid,
For closing the sample of the centrifuge tube body interior；Centrifuge tube transition lid, the centrifuge tube lid can be installed on
The centrifuge tube transition lid, the centrifuge tube transition cover body can be installed on the centrifugation tube body；Suction pipe is fixed on institute
State centrifuge tube transition lid and for extracts by centrifuge separation after positioned at the centrifuge tube body interior middle section or
The sample of bottom.
Preferably, the suction pipe extends to the bottom of the centrifuge tube body interior, and for extracting the centrifuge tube
The sample of the bottom of body interior.
Preferably, the suction pipe extends to the middle section of the centrifuge tube body interior, and for extract it is described from
The sample of middle section inside heart pipe.
Preferably, the internal diameter of the suction pipe is greater than or equal to 0.25cm and is less than or equal to 0.5cm.
Preferably, the centrifuge tube transition lid is provided with for the sample to be added one of the centrifugation tube body
Or multiple through-holes.
Preferably, the centrifuge tube lid and the centrifuge tube transition lid are connected through a screw thread or are interference fitted connection；
And/or the centrifuge tube transition lid and the centrifugation tube body are connected through a screw thread or are interference fitted connection.
Preferably, the suction pipe is higher by the upper surface of the centrifuge tube transition lid.
The present invention also provides a kind of centrifuge tube, the centrifuge tube includes: centrifugation tube body, is used to contain and be centrifugated
Sample；And centrifuge tube adapter according to the present invention, it can connect, be used for from the centrifuge tube with the centrifugation tube body
Body interior extracts the sample.
Preferably, the centrifugation tube body is conical centrifuge tube body.
The present invention also provides a kind of method for extracting sample, this method extracts warp using centrifuge tube according to the present invention
It is located at the middle section of centrifuge tube body interior or the sample of bottom after crossing centrifuge separation, the described method comprises the following steps:
The centrifuge tube transition lid for being fixed with suction pipe is installed on the centrifugation tube body, by being formed in by step A
The through-hole of the centrifuge tube transition lid sequentially adds density gradient into the centrifugation tube body and to spun specimen,
Alternatively, the centrifuge tube transition lid for being fixed with the suction pipe is first inserted into the centrifugation tube body, and incline
The oblique centrifuge tube transition lid, sequentially adds density gradient from the opening of the centrifugation tube body and to spun specimen,
Then the centrifuge tube transition lid is installed on the centrifugation tube body；
The centrifuge tube lid is installed on the centrifuge tube transition lid by step B；
The centrifuge tube is put into centrifuge, the scheduled time is centrifuged under scheduled centrifugal force by step C；
Step D opens the centrifuge tube lid, and another suction pipe that diameter is less than the diameter of the suction pipe is inserted into the suction
In pipe, another suction pipe is made to protrude into the bottom of the suction pipe to extract sample.
Centrifuge tube and centrifuge tube adapter of the invention can extract after density-gradient centrifugation method decomposes and be located at centrifuge tube sheet
The body middle section perhaps sample of bottom and extracted sample is not by the pollution of upper sample or other mixed liquors, and
And centrifugation adapter structure of the invention is simple, versatile, and conventional centrifuge tube can be cooperated to use.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of existing conventional centrifugal pipe；
Fig. 2 is the structural schematic diagram of centrifuge tube according to the present invention；
Fig. 3 is the cross-sectional view of centrifuge tube according to the present invention；
Fig. 4 is that centrifuge tube according to the present invention omits centrifuge tube lid and is centrifuged the structural schematic diagram of tube body；
Fig. 5 is the top view of the centrifuge tube transition lid of centrifuge tube of the invention according to Fig.2,；
Fig. 6 is the cross-sectional view of centrifuge tube adapter according to the present invention.
Description of symbols
100- centrifuge tube lid；200- centrifuge tube transition lid；210- external thread part；220- internal thread part；230- through-hole；
300- suction pipe；400- is centrifuged tube body.
Specific embodiment
Below with reference to the accompanying drawings centrifuge tube and centrifuge tube adapter of the invention are described in detail and are illustrated.
Fig. 1 is the structural schematic diagram of existing conventional centrifugal pipe.Existing conventional centrifugal pipe by centrifuge tube lid 100 and from
Heart tube body 400 form, extraction and application density-gradient centrifugation method separation after sample when, if required sample be located at from
When the bottom of heart tube body 400, the bottom that suction pipe can only be inserted into centrifugation tube body 400 be extracted, or by the mixing on upper layer
Liquid or the removal of other samples, sample needed for lower layer can be extracted.But above two operating method can all cause lower layer
The problem of pollution of the sample by upper layer mixed liquor or other samples.
Fig. 2 is the structural schematic diagram of centrifuge tube according to the present invention.Fig. 3 is the cross-sectional view of centrifuge tube according to the present invention.
Referring to figs. 2 and 3, centrifuge tube of the invention include centrifuge tube lid 100, centrifuge tube transition lid 200, suction pipe 300 and from
Heart tube body 400.As shown in figure 3, suction pipe 300 is fixed on centrifuge tube transition lid 200, and suction pipe 300 in present embodiment
Upper end be higher by the upper surface of centrifuge tube transition lid 200.It in this way can be to avoid sample and close be added to centrifugation tube body 400
When spending gradient liquid, perhaps density gradient flows into suction pipe 300 aliquot or the opening of suction pipe 300 is by sample
Pollution.Centrifuge tube lid 100 is tightened in centrifuge tube transition lid 200, the upper end of suction pipe 300 and centrifuge tube lid by screw thread
There are gaps between 100.
With reference to Fig. 4 and Fig. 5, centrifuge tube transition lid 200 have the external thread part 210 that is connect with centrifuge tube lid 100 and
The internal thread part 220 being connect with centrifugation tube body 400.From figure 5 it can be seen that there are two the settings of centrifuge tube transition lid 200
For adding the through-hole 230 of sample and density gradient.Centrifuge tube can be added in sample and density gradient by through-hole 230
Ontology 400.
As shown in figure 3, therefore the bottom that suction pipe 300 extends to centrifugation tube body 400 can directly be mentioned by suction pipe 300
The sample of centrifugation 400 bottom of tube body is taken, and extracted sample not will receive other examinations at centrifuge tube top or middle part
The pollution of sample or mixed liquor.Equally, suction pipe 300 can also extend to other any positions of centrifugation tube body 400, to extract
Required sample.
The internal diameter of suction pipe 300 is preferably greater than or equal to 0.25cm and is less than or equal to 0.5cm.If in suction pipe 300
Diameter is too small, and a large amount of gradient liquid can be sucked due to capillary phenomenon, in suction pipe 300, to influence the extraction of sample；If suction pipe
300 internal diameters are too big, then are unfavorable for the control of sample extracted amount.
Being centrifuged tube body 400 is preferably taper tube body.This is because if after centrifugation, the sample to be extracted
This is located at the bottom of centrifuge tube ontology and content is seldom, and the base diameter of taper tube body is less than the cylindrical tube sheet of same specification
The liquid level of the base diameter of body, the then sample to be extracted is higher relative to the liquid level of the cylindrical tube ontology of same specification, in this way
Convenient for the extraction of sample.
In the present embodiment, preferably, passing through screw thread between centrifuge tube lid 100 and centrifuge tube transition lid 200
Connection, also by threaded connection between centrifuge tube transition lid 200 and centrifugation tube body 400.Be threadedly coupled it is solid and reliable, be convenient for
Disassembly, while also can be avoided the splashing etc. of the sample in disassembly process.
Fig. 6 is the cross-sectional view of centrifuge tube adapter of the invention comprising centrifuge tube lid 100, centrifuge tube transition lid
200 and suction pipe 300.Suction pipe 300 is fixed on centrifuge tube transition lid 200, and the top of suction pipe 300 is higher by centrifugation tube transitions
The upper surface of lid 200.Centrifuge tube lid 100 is tightened in centrifuge tube transition lid 200 by screw thread, the upper end of suction pipe 300 and
There are gaps between centrifuge tube lid 100.
The external thread part 210 of centrifuge tube transition lid 200 in centrifuge tube adapter according to the present invention can be with routine
The external screw thread for being centrifuged tube body is identical, and the internal thread part 220 of centrifuge tube transition lid 200 can be with conventional centrifugal pipe lid
Internal screw thread is identical, in this way, centrifuge tube adapter of the invention can be directly applied for conventional centrifuge tube.
As a preferred solution of the present invention, centrifuge tube lid 100, centrifuge tube transition lid 200 and centrifugation tube body 400 it
Between be connected through a screw thread.It is understood that centrifuge tube lid 100, centrifuge tube transition lid 200 and centrifuge tube sheet in the present invention
It can also be connected by the plug-in of interference fit between body 400 or other connection types connect.
The application to centrifuge tube adapter of the invention in assisted reproductive technology is described in detail and illustrates below.
After separating sperm by density-gradient centrifugation method, the sample sperm after optimization is in the bottom of centrifuge tube.According to existing
There is common method in technology, when extracting lower layer's sample sperm, not can avoid upper layer refining to the sample sperm to be extracted
Pollution.Centrifuge tube of the invention, can be easily since it has the suction pipe 300 for extending to centrifugation 400 bottom of tube body
Sample sperm after extracting bottom optimization, while guaranteeing extracted sample sperm not by the pollution of upper layer refining.
The specifically used process of centrifuge tube of the invention in assisted reproductive technology are as follows: by centrifuge tube adapter of the invention
It is installed on centrifugation tube body 400, opens centrifuge tube lid 100.Density gradient centrifugation liquid is passed through into centrifugation according to the height of concentration
The through-hole 230 of tube transitions lid 200 is sequentially added in centrifugation tube body 400.Then initial sperm is proportionally passed through into centrifugation
The through-hole 230 of tube transitions lid 200 is added in centrifugation tube body 400.At this point, into the only density gradient inside suction pipe 300
Liquid.Centrifuge tube lid 100 is tightened in centrifuge tube transition lid 200.Finally centrifuge tube is placed in centrifuge, is centrifuged in 300g
Under power, centrifugation 20 minutes is carried out.Centrifuge tube lid 100 is opened, diameter is less than to another suction pipe (not shown) of suction pipe 300
It is inserted into the bottom of suction pipe 300, extracts the sample sperm after optimization from the bottom of suction pipe 300.It is of course also possible to directly use this hair
Bright centrifuge tube carries out density-gradient centrifugation method separation to original sperm.Since the lower end of suction pipe 300 is not in contact with original sperm, from
After heart separation, only it is located at the density gradient on 300 top of suction pipe and positioned at 300 lower part of suction pipe into inside suction pipe 300
Sample sperm after optimization, therefore extracted sample sperm not will receive the pollution of upper layer essence paddle.
In above process, if centrifuge tube transition lid 200 is not provided with through-hole 230, suction pipe will can be first fixed with
The 300 insertion centrifugation tube body 400 of centrifuge tube transition lid 200, and centrifuge tube transition lid 200 is tilted, by density gradient
Centrifugation tube body 400 is added from the opening of centrifugation tube body 400 according to the height of concentration in centrifugate, then in the same way
Proportionally initial sperm is added in centrifugation tube body 400 and is centrifuged.
Likewise, centrifuge tube and centrifuge tube adapter of the invention, can be applied to the density gradient of other mixed liquor from
In heart method isolation technics, if to extract the sample of centrifugation 400 middle section of tube body, it is only necessary to according to actual needs using suitable
When the suction pipe 300 of length.
In embodiment according to the present invention, there are two through-holes for setting on centrifuge tube transition lid 200, but should manage
Solution is that centrifuge tube transition lid 200 of the invention may be set to be other structures, such as engraved structure, as long as it can ensure that will
Suction pipe 300 is fixed on centrifuge tube transition lid 200, and one or more through-holes with addition sample and density gradient are
It can.
The present invention can be applied equally to nucleic acid, subcellular organelle other than it can be applied to above-mentioned assisted reproductive technology
And the extraction of the sample that can be separated by density-gradient centrifugation method of compound protein etc..
Centrifuge tube adapter of the invention can cooperate conventional centrifuge tube to use, and versatile, structure is simple, user
Just.Dividing in centrifuge tube by density-gradient centrifugation method can be extracted by using centrifuge tube or centrifuge tube adapter of the invention
From it is rear be located at centrifugation tube body middle section perhaps the sample of bottom and extracted sample not will receive upper layer or other
The pollution of the sample or mixed liquor of position.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Within the technical scope disclosed by the invention, any changes or substitutions that can be easily thought of by those familiar with the art, all
It is covered by the protection scope of the present invention.
Claims (7)
1. a kind of centrifuge tube adapter is used to that centrifugation tube body to be cooperated to be located at centrifuge tube after centrifuge separation using to extract
The middle section of body interior or the sample of bottom, which is characterized in that the centrifuge tube adapter includes:
Centrifuge tube lid is used to close the sample of the centrifuge tube body interior；
Centrifuge tube transition lid, the centrifuge tube lid can be installed on the centrifuge tube transition lid, the centrifugation tube transitions
Lid can be connected through a screw thread or be interference fitted the top open part for being installed on the centrifugation tube body；
Suction pipe is fixed on the centrifuge tube transition lid and is located at the centrifuge tube sheet after centrifuge separation for extracting
The internal middle section in portion or the sample of bottom,
The centrifuge tube transition lid is provided with one or more through-holes for the sample to be added to the centrifugation tube body,
The centrifuge tube transition lid has flat upper surface, and the suction pipe is higher by the upper table of the centrifuge tube transition lid
Face, when the centrifuge tube lid is installed on the centrifuge tube transition lid, the upper end of the suction pipe and the centrifuge tube lid
Between there are gaps.
2. centrifuge tube adapter according to claim 1, which is characterized in that the suction pipe extends to the centrifugation tube body
Internal bottom, and the sample of the bottom for extracting the centrifuge tube body interior.
3. centrifuge tube adapter according to claim 1, which is characterized in that the suction pipe extends to the centrifugation tube body
Internal middle section, and the sample for extracting the middle section inside the centrifuge tube.
4. centrifuge tube adapter according to claim 1, which is characterized in that the internal diameter of the suction pipe is greater than or equal to
0.25cm and be less than or equal to 0.5cm.
5. centrifuge tube adapter according to claim 1, which is characterized in that
The centrifuge tube lid and the centrifuge tube transition lid are connected through a screw thread or are interference fitted connection.
6. a kind of centrifuge tube, which is characterized in that the centrifuge tube includes:
It is centrifuged tube body, is used to contain and be centrifugated sample；
Centrifuge tube adapter according to any one of claims 1-5 can connect with the centrifugation tube body, be used for
The sample is extracted from the centrifuge tube body interior.
7. centrifuge tube according to claim 6, which is characterized in that the centrifugation tube body is conical centrifuge tube body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710235804.3A CN107008518B (en) | 2017-04-12 | 2017-04-12 | Centrifuge tube, centrifuge tube adapter and the method for extracting sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710235804.3A CN107008518B (en) | 2017-04-12 | 2017-04-12 | Centrifuge tube, centrifuge tube adapter and the method for extracting sample |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107008518A CN107008518A (en) | 2017-08-04 |
| CN107008518B true CN107008518B (en) | 2019-08-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710235804.3A Active CN107008518B (en) | 2017-04-12 | 2017-04-12 | Centrifuge tube, centrifuge tube adapter and the method for extracting sample |
Country Status (1)
| Country | Link |
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| CN (1) | CN107008518B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108786950B (en) * | 2018-03-30 | 2020-09-22 | 江苏科华医疗器械科技有限公司 | Liquid centrifuging tube is got to over-and-under type |
| CN112295629A (en) * | 2019-08-02 | 2021-02-02 | 上海唯问生物技术有限公司 | Centrifuge tube |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2198606A1 (en) * | 1994-08-31 | 1996-03-07 | Peter Van Vlasselaer | Centrifuge syringe apparatus and method |
| JP2002116201A (en) * | 2000-10-11 | 2002-04-19 | Sekisui Chem Co Ltd | Cell-function measuring container |
| CN104651224A (en) * | 2015-02-10 | 2015-05-27 | 张丽红 | Double-tube vacuum film sperm separation method and separation device |
| CN204768798U (en) * | 2015-06-30 | 2015-11-18 | 天津市康婷生物工程有限公司 | Filterable suit centrifuging tube |
| CN205258465U (en) * | 2015-11-06 | 2016-05-25 | 山东省计划生育科学技术研究所 | Sperm separator |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2721094Y (en) * | 2004-07-21 | 2005-08-31 | 张长青 | Centrifugal tube for preparing blood cell enriched plasma |
-
2017
- 2017-04-12 CN CN201710235804.3A patent/CN107008518B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2198606A1 (en) * | 1994-08-31 | 1996-03-07 | Peter Van Vlasselaer | Centrifuge syringe apparatus and method |
| JP2002116201A (en) * | 2000-10-11 | 2002-04-19 | Sekisui Chem Co Ltd | Cell-function measuring container |
| CN104651224A (en) * | 2015-02-10 | 2015-05-27 | 张丽红 | Double-tube vacuum film sperm separation method and separation device |
| CN204768798U (en) * | 2015-06-30 | 2015-11-18 | 天津市康婷生物工程有限公司 | Filterable suit centrifuging tube |
| CN205258465U (en) * | 2015-11-06 | 2016-05-25 | 山东省计划生育科学技术研究所 | Sperm separator |
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| Publication number | Publication date |
|---|---|
| CN107008518A (en) | 2017-08-04 |
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