CN203523573U - Lasioderma serricorne breeding box - Google Patents
Lasioderma serricorne breeding box Download PDFInfo
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- CN203523573U CN203523573U CN201320579103.9U CN201320579103U CN203523573U CN 203523573 U CN203523573 U CN 203523573U CN 201320579103 U CN201320579103 U CN 201320579103U CN 203523573 U CN203523573 U CN 203523573U
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- lasioderma serricorne
- humidity
- cigarette beetle
- incubator
- control system
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Abstract
The utility model belongs to the technical field of insect control, and particularly relates to a Lasioderma serricorne breeding box and a method for enriching Lasioderma serricorne infecting microorganisms through the Lasioderma serricorne breeding box. The Lasioderma serricorne breeding box comprises a growth box, a water tank, a temperature control system and a humidity control system. The method for enriching the Lasioderma serricorne infecting microorganisms through the Lasioderma serricorne breeding box includes several steps such as the breeding step, the inoculation step, the collection step and the pathogenetic insect body grinding step. The Lasioderma serricorne breeding box is simple, the temperature and the humidity of the Lasioderma serricorne breeding box can be precisely controlled so as to meet the requirements of Lasioderma serricorne for temperature and humidity in various growth stages, and a large number of Lasioderma serricorne adults can be bred. The method for enriching the Lasioderma serricorne infecting microorganisms through the Lasioderma serricorne breeding box is simple, high in speed and effective; collected powder is a mixture of the Lasioderma serricorne and the Lasioderma serricorne infecting microorganisms, is safe and nontoxic and can be repeatedly used for infecting new Lasioderma serricorne, and therefore the method for enriching the Lasioderma serricorne infecting microorganisms through the Lasioderma serricorne breeding box is environmentally friendly and economical.
Description
Technical field
The invention belongs to insect control technical field, be specifically related to the incubator of a kind of cigarette beetle.
Background technology
Cigarette beetle (Lasioderma serricorne) belongs to Insecta, coleoptera, is a kind of global stored product insect.Cigarette beetle feeding habits are complicated, endanger the grains such as cereal, beans, oil plant in grain depot; The tobacco leaf of harm storage in tobacco leaf warehouse, the tobacco products such as cigarette and cigar.The growth optimum condition of cigarette beetle is 30 ℃ of temperature and relative moisture 70%, completes its life Zhou Yuexu 24 days under this environment.Each female worm can lay eggs more than 100 all one's life, and ovum produces in tobacco leaf gap or grain grain recess, lays eggs one at every turn.Cigarette beetle is very big for the tobacco epidemic of storage, and Tobacco Product Quality is had to very important impact.Before 2000, the phenomenon that domestic tobacco enterprise causes cigarette quality to decline to cigarette beetle is paid little attention to, and oil smoke, the events such as cigarette of damaging by worms often have generation.In recent years, along with the variation of the technological transformation of cigarette factory, processing technology, environment, the raising that product quality requires, the harmfulness of cigarette beetle and the importance of control being familiar with by people progressively.
In prior art, for effectively preventing and treating cigarette beetle, the method that multiplex aluminum phosphide fumigation sprays in conjunction with chrysanthemum ester is carried out integrated control to cigarette beetle, controls effect comparatively obvious.But medicine aluminum phosphate used health to operating personnel in operation process has potential safety hazard, and during operation, impact is normal produces, and chrysanthemum ester class chemicals has the risk at tobacco product Pesticide Residues, so the method for this class chemical control cigarette beetle is quite disputed on.
In recent years, the research that utilizes ecological law to carry out biological control to all kinds of damage by disease and insect is in the ascendant, and its major advantage is safety, environmental friendliness.Utilize microorganism to carry out the more existing positive explorations of ecological prevention and control to cigarette beetle; as Zou Xiao etc. thinks that white muscardine fungi has certain effect (Zou Xiao for prevention and control cigarette beetle; Zhang Xiaomin etc.; 4 kinds of entomogenous fungis lethal effect that infects to cigarette beetle; plant protection; 2008,34(2): 40 ~ 43).But in prior art, lack the enrichment method that microorganism after cigarette beetle is infected in a kind of incubator for cigarette beetle and enrichment.
Summary of the invention
The object of the invention is to provide the incubator of a kind of cigarette beetle and utilizes this device enrichment to infect cigarette beetle method of microorganism.
The present invention is achieved through the following technical solutions the object of the invention.
A cigarette beetle incubator, comprises growth case, water tank, temperature control system and 4 parts of moisture control system, and it is inner that water tank is positioned at growth box body, and temperature control system and moisture control system are controlled respectively the temperature and humidity of growth box body inside.
Described cigarette beetle incubator, growth box body has upper cover, can open and seal, and tank box has upper cover, can open and seal.
Described cigarette beetle incubator, tank box upper cover is porose, and cellulose acetate filter rod immerses water body in water tank by this hole, with this moisture that volatilizees, increases humidity in growth box body.
Described cigarette beetle incubator, the water of temperature control heating rod heating water tank of temperature control system by being positioned at tank box is so that growth casing temperature reaches preset value; Moisture control system is controlled fan by digital display humidity and is controlled the humidity in growth casing.
Described cigarette beetle incubator, moisture control system is equipped with 300 mesh sieve thin,tough silk with the air inlet place that digital display humidity is controlled fan, can prevent polypide flight.
Utilize the enrichment of above-mentioned cigarette beetle incubator to infect an enrichment method for cigarette beetle microorganism, comprise the following steps:
(1) incubator is carried out to sterilization, the aseptic ovum of cigarette beetle is placed in to incubator bottom, by cigarette beetle required temperature and humidity conditions of the history of life in each stage, cigarette beetle is cultivated, cultivate into Sterilized health polypide standby;
(2), in proportion to cigarette beetle microbe inoculation, under 25 ℃, 90% damp condition, cultivate 2 ~ 3 days;
(3) after polypide morbidity, collect morbidity polypide, air-dry grinding, obtains powder.
Each Life Stages temperature and humidity conditions of the described cigarette beetle of step (1) is: ovum hatching, and 32 ℃, 75% humidity, hatches 2 ~ 3 days; Larva, to the adult stage, comprises pupa time, and 25 ℃, 60% humidity, cultivates 18 days, until grow up to adult.
Step (2) is described is evenly sprayed on cigarette beetle polypide to cigarette beetle inoculation for microbial spore or microorganism uniform spreading are spread on to incubator bottom or make suspension.
Step (3) the polypide incidence of disease is selected, after 75% polypide morbidity, to collect morbidity polypide.
After the air-dry grinding of step (3), gained powder was selected 200 mesh sieves.
Cigarette beetle incubator provided by the present invention, each several part equipment is easy to buy from market, can incubator body temperature humidity be realized comparatively accurately and being controlled, meet the requirement in each stage of cigarette beetle growth, can cultivate a large amount of aseptic cigarette beetle adults.The enrichment method that utilizes incubator enrichment to infect cigarette beetle microorganism provided by the present invention, method is simple, fast effectively, collected powder is cigarette beetle and infects the mixture of cigarette beetle microorganism, safe, nontoxic, can be recycled and reused for and can infect new cigarette beetle, comparatively environmental protection and economy.
Accompanying drawing explanation
Fig. 1 is cigarette beetle incubator schematic diagram;
In figure, represent that content is respectively: 1, growth case; 2, water tank; 3, distilled water; 4, temperature control heating rod; 5, cellulose acetate filter rod; 6, digital display humidity is controlled fan; 7, power supply; 8, feed; 9, cigarette beetle.
Embodiment
Below in conjunction with embodiment the present invention will be further explained explanation.
embodiment 1
The special equipment that case is ectogenesis cigarette beetle is grown in cigarette beetle training, and as shown in Figure 1, cigarette beetle incubator comprises growth case 1, water tank 2, temperature control heating rod 4 and digital display humidity control fan 6 to its concrete structure; Wherein in water tank 2, be added with distilled water 3, temperature control heating rod 4 is arranged in water tank 2, by adding hot distilled water 3, carrys out temperature in growth regulation case 1 casing; Cellulose acetate filter rod 5 invades in distilled water 3 by the aperture covering on water tank 2, with this, increases humidity in growth 1 casing; Digital display humidity is controlled fan 6 and is connected with power supply 7, according to preset value, carrys out the humidity in growth regulation case 1 casing, at the air inlet of fan 6,300 mesh sieve thin,tough silk is housed, and can prevent polypide flight.
The distilled water 3 of incubator inner water tank 2 splendid attires, height of water level is about 2/3rds of water tank 2.Temperature control heating rod 4 is immersed in distilled water 3 completely, and the heated perimeter in winter can reach 20 ~ 40 ℃.Because specific heat of water is very large, such system makes whole training grow can be the as required constant upper and lower temperature difference scope at 2 ℃ of the temperature of case.The original effect of cellulose acetate filter rod 5 is mainly used in being processed into cylindrical bar cigarette smoke to filtration, it is the filter tip of filter tip cigarette, in this device, mainly utilize its good water imbibition and volatility, the moisture evaporation in water tank is grown in case to training, increase the humidity that case is grown in training.In growth case 1 casing of incubator, stack the feeds 8 such as pipe tobacco or cereal, provide cigarette beetle 9 growth desired nutritional materials, also as cigarette beetle, complete the place of whole history of life of process herein.
embodiment 2
Utilize cigarette beetle incubator enrichment described in embodiment 1 to infect the enrichment method of cigarette beetle microorganism, comprise the following steps:
(1) incubator is carried out to sterilization, the aseptic ovum of cigarette beetle is placed in to incubator bottom, according to cigarette beetle required temperature and humidity conditions of the history of life in each stage, tobacco process is cultivated, utilize the prepared cigarette beetle incubator of embodiment 1, can realize temp. and humidity and control comparatively accurately.Wherein the ovum hatching stage, 32 ℃, 75% humidity, hatch and can hatch larva in 2 ~ 3 days, and the time required compared with natural conditions shortens dramatically; Larva, to the adult stage, comprises pupa time, 25 ℃, 60% humidity, cultivates 18 days until adult, and compared with needing 24 days under natural conditions, even the longer time also shortens dramatically.The healthy polypide that cultivation is completed is standby.
(2) for cigarette beetle inoculation, estimate microbial infection in proportion, under 25 ℃, 90% damp condition, cultivate 2 ~ 3 days; Described inoculation is in proportion prior art content, and because of microbe inoculation difference, inoculum concentration is distinguished to some extent; Described inoculation is for being spread on microbial spore uniform spreading incubator bottom or making suspension and evenly spray and cigarette beetle polypide.
(3) after polypide morbidity, select to regather morbidity polypide after 75% polypide morbidity, now infect the microbial biomass of cigarette beetle compared with horn of plenty; The air-dry grinding of morbidity polypide that collection is obtained, obtains mixed-powder, and gained mixed-powder is crossed to 200 mesh sieves.
Finally gained mixed-powder is cigarette beetle and infects the mixture of cigarette beetle microorganism, can be for microbe species and the concentration thereof of the suitableeest infection of screening cigarette beetle for this mixture.Because the whole preparation process of this mixture all belongs to sterile working, the mixed-powder therefore finally making is safe, nontoxic.After filtering out the suitableeest infection cigarette beetle microbe species and concentration thereof, the powder of preparing by the method can be recycled and reused for and can infect new cigarette beetle, so the method also has advantages of economic environmental protection, be suitable for applying.
Claims (5)
1. a cigarette beetle incubator, it is characterized in that: this incubator comprises growth case, water tank, temperature control system and 4 parts of moisture control system, it is inner that water tank is positioned at growth box body, and temperature control system and moisture control system are controlled respectively the temperature and humidity of growth box body inside.
2. cigarette beetle incubator as claimed in claim 1, is characterized in that: growth box body, tank box are equipped with the upper cover that can open or seal.
3. cigarette beetle incubator as claimed in claim 1, is characterized in that: tank box upper cover is porose, and cellulose acetate filter rod immerses water body in water tank by this hole, with this moisture that volatilizees, increases humidity in growth box body.
4. cigarette beetle incubator as claimed in claim 1, is characterized in that: the water of temperature control heating rod heating water tank of temperature control system by being positioned at tank box is so that growth casing temperature reaches preset value; Moisture control system is controlled fan by digital display humidity and is controlled the humidity in growth casing.
5. cigarette beetle incubator as claimed in claim 4, is characterized in that: moisture control system is equipped with 300 mesh sieve thin,tough silk with the air inlet place that digital display humidity is controlled fan.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320579103.9U CN203523573U (en) | 2013-09-18 | 2013-09-18 | Lasioderma serricorne breeding box |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320579103.9U CN203523573U (en) | 2013-09-18 | 2013-09-18 | Lasioderma serricorne breeding box |
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| Publication Number | Publication Date |
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| CN203523573U true CN203523573U (en) | 2014-04-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201320579103.9U Expired - Lifetime CN203523573U (en) | 2013-09-18 | 2013-09-18 | Lasioderma serricorne breeding box |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478082A (en) * | 2013-09-18 | 2014-01-01 | 河南中烟工业有限责任公司 | Lasioderma serricorne breeding chamber and method for gathering invasion microorganisms by aid of same |
| CN109152351A (en) * | 2016-05-20 | 2019-01-04 | 恩托普洛特公司 | For cultivating the device and method of invertebrate |
-
2013
- 2013-09-18 CN CN201320579103.9U patent/CN203523573U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478082A (en) * | 2013-09-18 | 2014-01-01 | 河南中烟工业有限责任公司 | Lasioderma serricorne breeding chamber and method for gathering invasion microorganisms by aid of same |
| CN109152351A (en) * | 2016-05-20 | 2019-01-04 | 恩托普洛特公司 | For cultivating the device and method of invertebrate |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP02 | Change in the address of a patent holder |
Address after: 450000 Yulin South Road, Henan, Zheng Dong, No. 16 South Road, Zhengzhou Patentee after: CHINA TOBACCO HENAN INDUSTRIAL Co.,Ltd. Address before: 450000 Henan province Zhengzhou guanchengou Longhai Road No. 72 (Zhengzhou factory) Patentee before: CHINA TOBACCO HENAN INDUSTRIAL Co.,Ltd. |
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| CX01 | Expiry of patent term |
Granted publication date: 20140409 |
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| CX01 | Expiry of patent term |