CN202599958U

CN202599958U - Kit for protease 3 and myeloperoxidase combination detection

Info

Publication number: CN202599958U
Application number: CN 201220235365
Authority: CN
Inventors: 王高升; 王学超; 张晨磊; 付金瑞; 李会萍; 孙雪莲
Original assignee: Tianjin Baodi Hospital
Current assignee: Tianjin Baodi Hospital
Priority date: 2012-05-24
Filing date: 2012-05-24
Publication date: 2012-12-12
Anticipated expiration: 2022-05-24

Abstract

The utility model relates to a kit for protease 3 and myeloperoxidase combination detection. A kit body is a rectangular, and is connected with a kit cover. A reagent support made of stearin foamed plastic is placed in the kit body. The reagent support is provided with an A rectangular groove and a B rectangular groove, wherein the A rectangular groove and the B rectangular groove are respectively used for holding a cover glass box which holds cover glass and is made of hard paper, and a reaction plate. One fifth of the left side of the reaction plate undergoes brush finish. One side of the cover glass is provided with small points to protect reagents in a reaction area. The reagent support is further provided with five circular grooves which are respectively used for holding biotin, streptavidin, specimen diluents, wash concentrate and mounting medium. Four reaction points in the reaction area are respectively provided with different reagents in a dotting mode. The kit has the advantages that the kit for the protease 3 and myeloperoxidase combination detection is applied to indirect immunofluorescence of a biotin-streptavidin system; all the reagents are placed together for convenient use; the detection is fast and convenient, reliable and sensitive; experimental space is saved; and misoperation is avoided.

Description

Protease 3 and myeloperoxidase combine detection are used kit

Technical field

A kind of medical science that relates to the utility model detects and uses kit, and particularly a kind of detection is convenient, and convenient in application is reliable, and sensitivity is saved the protease 3 and the myeloperoxidase combine detection of lab space and used kit.

Background technology

As everyone knows, at present, the associated antibodies that detects protease 3 and myeloperoxidase in hospital and other relevant medical testing agencies is independent detection; Operating comparatively wastes time and energy, and because the inconsistent of operation steps all might produce certain deviation, the detection method in past is mostly used the indirect enzyme-linked immunosorbent method; Resultant error is big; Sensitivity is lower, and reagent is placed scattered, takes by mistake easily to cause the accident.

Summary of the invention

The utility model provides a kind of detection sensitivity high for solving the technical matters that exists in the known technology; Reliable results; Easy to operate, will detect separately and be converted into combine detection, save the protease 3 and the myeloperoxidase combine detection in running time and place and use kit.

The technical scheme that the utility model is taked for the technical matters that exists in the solution known technology is: it comprises box body, lid and reagent holder; It is characterized in that: the reagent box body is a rectangle; And be connected with lid; Place the reagent holder of processing in the reagent box body by stearic polyfoam; Have two A, B rectangular recess in the described reagent holder, what be respectively applied for dress cover glass in depositing processes cover glass box and reaction egative film with the hard papery, also has five circular grooves in the reagent holder and is respectively applied for and deposits biotin, streptavidin, sample diluent, concentrated washing lotion and mountant.

The utility model can also adopt following technical scheme:

Describedly process the cover glass box with the hard papery, the plastic foam plate of its liner 0.2 millimeters thick, the cover glass size in the lid glass box is suitable with the reaction egative film, and a side of cover glass is provided with outstanding point triangular in shape;

There is the poly-D-lysine coating on 4/5 surface, described reaction egative film right side; Four reaction areas on the coating is processed with polyester paint; The layout of each reaction zone is identical, and left upper point is gone up the protease 3 of 0.5mm in the reaction zone, and lower right-hand corner point is gone up the protein solution of 0.5mm; Place, upper right corner point is gone up the 0.5mm myeloperoxidase, and place, lower right corner point is gone up 0.5mm protease 3 antigen and positive Antiproteinase 3 antibody serum mixture; Left side 1/5 is a frosting;

Every on described reaction egative film is packed interior dress drying agent with plastic sheeting is independent sealed;

Described biotin is rat anti human IgG antibody and biotin labeling thing;

Described biotin, streptavidin, sample diluent, concentrated washing lotion and mountant all adopt round plastic bottle packing, and have bottle cap;

Described streptavidin adopts the label of luciferin and streptavidin;

Described four reaction areas is that the square 5mm of being of a size of * 5mm or circular dimension are ∮ 5mm.

Advantage and the good effect that the utlity model has are: protease 3 and myeloperoxidase combine detection kit are applied to biotin-streptavidin system IIF; All reagent are all concentrated convenient in application together; It is convenient to detect, reliable, sensitivity; Save lab space, avoided maloperation.

Description of drawings

Fig. 1 is the utility model one-piece construction synoptic diagram.

Among Fig. 2: 1. reagent box body, 2 lids, 3 reagent holders, 4.A rectangular recess, 5.B rectangular recess; 6. cover glass box, the 6-1 cover glass, 6-2. gives prominence to point, 7. reaction egative film, 8 biotins; 9. streptavidin, 10. sample diluent, 11. concentrate washing lotions, 12. mountant, 13-16. reaction zone; 17. the upper left corner, 18. lower left corners, 19. lower right corner, 20. upper right corner.

Embodiment

For further understanding summary of the invention, characteristics and the effect of the utility model, the following examples of giving an example now, and conjunction with figs. specifies as follows, sees also Fig. 1, Fig. 2.

As shown in Figure 1: reagent box body 1 is a rectangle; And be connected with lid 2; The reagent holder 3 that the 1 interior placement of reagent box body is processed by stearic polyfoam has two A, B rectangular recess 4,5 in the described reagent holder 3, be respectively applied for and adorn processing cover glass box 6 and react egative film 7 with the hard papery of cover glass 6-1 in depositing; Also having five circular grooves in the reagent holder 3 is respectively applied for and deposits biotin 8, streptavidin 9, sample diluent 10, concentrates washing lotion 11 and mountant 12; They all adopt round plastic bottle packing, and have bottle cap, and wherein said streptavidin 9 adopts the label of luciferin and streptavidin; Describedly process cover glass box 6 with the hard papery; The plastic foam plate of its liner 0.2 millimeters thick; To prevent attrition crushing; Cover glass 6-1 size in the cover glass box 6 is suitable, as shown in Figure 3 with reaction egative film 7: the side of cover glass 6-1 is provided with outstanding point 6-2 triangular in shape, for the reagent in the reaction zone on the protective reaction egative film 7; As shown in Figure 2: there is the poly-D-lysine coating on 4/5 surface, described reaction egative film 7 right sides; Four

reaction areas

13,14,15,16 usefulness polyester paint on the coating is processed; The square 5mm of being of a size of * 5mm (or circular dimension is ∮ 5mm); The layout of each reaction zone is identical, and the upper left corner 17 place's points are gone up the protease 3 of 0.5mm in the reaction zone, and the lower left corner 18 place's points are gone up the protein solution (myeloperoxidase, protease 3 detect negative) of 0.5mm; The upper right corner 20 place's points are gone up the 0.5mm myeloperoxidase, and the lower right corner 19 place's points are gone up 0.5mm protease 3 antigen and positive Antiproteinase 3 antibody serum mixture; The left side 1/5 is a frosting so that hand gets, described reaction egative film 7 every pack interior dress dryer damp-proofing with plastic sheeting is independent sealed; Described biotin 8 is rat anti human IgG antibody and biotin labeling thing;

During operation, open the lid 2 of kit 1, take out reaction egative film 7 by detecting specimen amount; Detect sample and carry out dilution in 1: 10, sample 30 microlitres after the dilution are joined in the reaction zone room temperature reaction 30 minutes with sample diluent 10; To concentrate washing lotion 11 and carry out dilution in 1: 100 and process the application washing lotion, wash reacting egative film 7 with using washing lotion then, add biotin 8 totally 30 microlitres again; Reacted 30 minutes, and reaction egative film 7 was washed again, add streptavidin 14 again with using washing lotion; Reacted 30 minutes, and reaction egative film 7 was washed again, in reaction zone, drip a mountant 12 with using washing lotion; Get a slice cover glass in the cover glass box 6 and cover on reaction egative film 7 observation under fluorescent microscope, judged result.

Its advantage is: protease 3 and myeloperoxidase combine detection kit are applied to biotin-streptavidin system IIF, and all reagent are all concentrated convenient in application together, and it is convenient to detect; Reliably; Sensitivity is saved lab space, has avoided maloperation.

Above-described embodiment describes the preferred implementation of the utility model; Be not that scope to the utility model limits; Under the prerequisite that does not break away from the utility model design spirit; Various distortion and improvement that those of ordinary skills make the technical scheme of the utility model all should fall in the definite protection domain of the utility model claims.

Claims

1. protease 3 and myeloperoxidase combine detection are used kit; It comprises box body, lid and reagent holder; It is characterized in that: reagent box body (1) is rectangle; And be connected with lid (2), and place the reagent holder (3) of processing in the reagent box body (1) by stearic polyfoam, have two A, B rectangular recess (4,5) in the described reagent holder (3); Be respectively applied for the processing cover glass box (6) and react egative film (7) of dress cover glass (6-1) in depositing, also have five circular grooves in the reagent holder (3) and be respectively applied for and deposit biotin (8), streptavidin (9), sample diluent (10), concentrated washing lotion (11) and mountant (12) with the hard papery.

2. protease 3 according to claim 1 and myeloperoxidase combine detection are used kit; It is characterized in that: describedly process cover glass box (6) with the hard papery; The plastic foam plate of its liner 0.2 millimeters thick; Cover glass (6-1) size in the cover glass box (6) is suitable with reaction egative film (7), and a side of cover glass (6-1) is provided with outstanding point (6-2) triangular in shape.

3. protease 3 according to claim 1 and myeloperoxidase combine detection are used kit; It is characterized in that: there is the poly-D-lysine coating on 4/5 surface, described reaction egative film (7) right side; Four reaction areas on the coating (13,14,15,16) is processed with polyester paint; The layout of each reaction zone is identical, and the protease 3 of 0.5mm is located a little to go up in the upper left corner (17) in the reaction zone, and the protein solution of 0.5mm is located a little to go up in the lower left corner (18); The 0.5mm myeloperoxidase is located a little to go up in the upper right corner (20), and 0.5mm protease 3 antigen and positive Antiproteinase 3 antibody serum mixture are located a little to go up in the lower right corner (19); Left side 1/5 is a frosting.

4. protease 3 according to claim 1 and myeloperoxidase combine detection are used kit, it is characterized in that: every on described reaction egative film (7) is packed interior dress drying agent with plastic sheeting is independent sealed.

5. protease 3 according to claim 1 and myeloperoxidase combine detection are used kit, it is characterized in that: described biotin (8) is rat anti human IgG antibody and biotin labeling thing.

6. protease 3 according to claim 1 and myeloperoxidase combine detection are used kit; It is characterized in that: described biotin (8), streptavidin (9), sample diluent (10), concentrated washing lotion (11) and mountant (12) all adopt round plastic bottle packing, and have bottle cap.

7. protease 3 according to claim 1 and myeloperoxidase combine detection are used kit, it is characterized in that: described streptavidin (9) adopts the label of luciferin and streptavidin.

8. protease 3 according to claim 1 and myeloperoxidase combine detection are used kit, it is characterized in that: described four reaction areas (13,14,15,16) is that the square 5mm of being of a size of * 5mm or circular dimension are ∮ 5mm.