CN205449993U - Autoimmune liver disease antibody spectrum detection kit - Google Patents
Autoimmune liver disease antibody spectrum detection kit Download PDFInfo
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- CN205449993U CN205449993U CN201620188279.5U CN201620188279U CN205449993U CN 205449993 U CN205449993 U CN 205449993U CN 201620188279 U CN201620188279 U CN 201620188279U CN 205449993 U CN205449993 U CN 205449993U
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Abstract
The utility model provides an autoimmune liver disease antibody spectrum detection kit, including external packing box, placed reagent bottle holder and receiver in the external packing box, the receiver upper surface is provided with recess I, and the description is put in the recess I, the left end face of receiver is provided with side channel I, the interpretation template place in in the side channel I, the right -hand member face of receiver is provided with side channel II, the accessory hold in the palm place in in the side channel II, reagent bottle holder includes the reagent bottle holder body, and the center of reagent bottle holder body is provided with incubation groove, and the upper surface of reagent bottle holder body divides and two sets ofly is provided with six reagent of putting reagent side by side and puts the through -hole, III bottles concentrated buffer solution bottles, enzyme conjugates bottle, colouring agent bottle, I bottle blocking agent, II bottles blocking agents and blocking agents put respectively in reagent is put in the through -hole. This autoimmune liver disease antibody spectrum detection kit simple structure, small in size conveniently hand -carry, can lump autoimmune liver disease antibody spectrum detection reagent and measuring instrument in order.
Description
Technical field
This utility model relates to a kind of test kit, particularly to a kind of autoimmune liver disease antibody repertoire detection kit.
Background technology
Autoimmune liver disease (autoimmuneliverdisease, AILD) it is one group of liver extremely mediated by autoimmune, gallbladder damages, according to its clinical manifestation, immunology, biochemical, iconography and Histopathological characteristics, can be classified as with hepatitis as principal mode, i.e. autoimmune hepatitis (autoimmunehepatitis, AIH), damage with stones in intrahepatic bile duct and cholestasis is as principal mode, i.e. primary biliary cirrhosis (primarybiliarycirrhosis, and primary sclerosing cholangitis (primarysclerosingcholangitis PBC), PSC).Additionally, the also Overlap syndrome (overlapsyndromes) of any two kinds in these three disease, wherein the most common with AIH-PBC Overlap syndrome.These diseases are all the liver chronic inflammation diseases mediated by autoimmune, can behave as serious hepatic lesions, and finally progress to liver cirrhosis.
Autoimmune liver disease antibody repertoire detection kit patients with autoimmune hepatitis spectrum in external qualitative detection serum.Restructuring or highly purified monoclonal antibody former AMAM2, Sp100, LKM1, gp210, LC1 and SLA are fixed on nitrocellulose filter in ribbon form.During for detecting, for avoiding non-specific binding occurs in inspection, first nitrocellulose filter is carried out protein blocking, add blood serum sample and hatch.If sample exists corresponding antibody, then specific binding with corresponding target antigen during hatching, (Ag-Ab) complex formed is fixed on nitrocellulose filter ad-hoc location, removes the partial antibody of uncombined (or unstable non-specific binding) after rinsing；Add the goat anti-human igg antibody's (enzyme conjugates) through horseradish peroxidase-labeled, after hatching, form Ag-Ab-anti antibody multienzyme complex, after rinsing, remove uncombined part enzyme labelled antibody；Adding colourless developer (TMB), through hatching, TMB embeds heart channel enzyme catalysis in peroxidase activity and becomes blue；Removing developer and add the reaction of distilled water color development stopping, absorbent paper compares interpretation card after blotting film bar and carries out testing result interpretation.
Relate to concentrate buffer, enzyme conjugates, developer, the many bottles of kinds of experiments reagent such as sealer and distilled water during the detection of autoimmune liver disease antibody repertoire, the most also include the instrument that the multiple needs such as incubation slot, detection membrane bar, tweezers, interpretation template and description are used, and the current commonly used simple packaging of autoimmune liver disease antibody repertoire detection kit, detectable and detection instrument are put unordered, in test kit carrying or moving process, easily cause the mutual collision between reagent bottle, be likely to result in the damage of experiment reagent.
Utility model content
The purpose of this utility model is to provide a kind of compact, simple in construction, the detectable that can relate to during being detected by autoimmune liver disease antibody repertoire and the autoimmune liver disease antibody repertoire detection kit of the orderly lump of detection instrument.
nullFor realizing technique scheme，A kind of autoimmune liver disease antibody repertoire detection kit that this utility model provides，Including external packing box，Reagent rack and receiver it is placed with in described external packing box，It is placed in parallel about described receiver and reagent rack，Described receiver upper surface is provided with groove I，Description is placed in described groove I，The left side of receiver is provided with side channel I，Interpretation template is positioned in described side channel I，The right side of receiver is provided with side channel II，Accessory props up and is placed in described side channel II，Described reagent rack includes I-shaped reagent rack body，Described reagent rack body be provided centrally with incubation slot，PVC liner plate it is pasted with above described incubation slot，The upper surface of described PVC liner plate divides two groups to be arranged side by side ten circles to hatch hole，Often group includes that five circles hatch hole，The upper surface of described reagent rack body divides two groups to be arranged side by side six reagent putting reagent to put through hole，Often group includes that through hole put by three reagent，Concentrate buffer bottle、Enzyme conjugates bottle、Developer bottle、Sealer I bottle、Sealer II bottle and sealer III bottle are placed in described reagent respectively and put in through hole.
In technique scheme, when using this autoimmune liver disease antibody repertoire detection kit, only the upper cover of external packing box need to be opened, receiver and reagent rack are taken out from external packing box, then interpretation template and accessory the torr side channel I from receiver and side channel II respectively are taken out, i.e. can be taken off the detection instrument that is placed in accessory torr and the experiment reagent being placed in reagent rack carries out test experience so that whole experimentation is convenient succinct.
Preferably, the upper surface of described accessory torr is provided with two parallel rectangular recess II spaced apart, two groups of detection membrane bars are respectively placed in described rectangular recess II, the side of described rectangular recess II is provided with V-shaped groove, tweezers are positioned in described V-shaped groove, tweezers and detection membrane bar are all instruments conventional in gastrointestinal disease antibody repertoire test experience, are desirable to separately put with reagent bottle, with the purpose reaching experiment reagent and experimental tool is separately put.
Preferably, described PVC liner plate hides 4/5ths of incubation slot at length direction, and it is whole that PVC liner plate hides incubation slot at width, and PVC liner plate can facilitate PVC liner plate paste in incubation slot and split at length direction slightly shorter than incubation slot.
This utility model having the beneficial effects that compared to prior art: the autoimmune liver disease antibody repertoire detection kit simple in construction of this utility model offer, compact, handy to carry about, can be by autoimmune liver disease antibody repertoire detectable and the detection orderly lump of instrument so that the detection of autoimmune liver disease antibody repertoire becomes convenient succinct.
Accompanying drawing explanation
Fig. 1 is perspective view of the present utility model.
Fig. 2 is the perspective view of reagent rack in this utility model.
Fig. 3 is the stereochemical structure decomposing schematic representation of receiver in this utility model.
In figure: 10, external packing box；20, reagent rack；30, receiver；201, reagent rack body；202, incubation slot；203, PVC liner plate；204, hole is hatched；205, buffer bottle is concentrated；206, enzyme conjugates bottle；207, developer bottle；208, sealer I bottle；209, sealer II bottle；210, sealer III bottle；301, groove I；31, description；32, interpretation template；33, accessory torr；331, groove II；332, V-shaped groove；40, detection membrane bar；50, tweezers.
Detailed description of the invention
Below in conjunction with the accompanying drawing in this utility model embodiment, the technical scheme in this utility model embodiment is carried out clear, complete description, it is clear that described embodiment is only a part of embodiment of this utility model rather than whole embodiments.The every other embodiment that this area ordinary person is obtained under not making creative work premise, belongs to protection domain of the present utility model.
Embodiment 1: a kind of autoimmune liver disease antibody repertoire detection kit.
nullReferring to figs. 1 through shown in Fig. 3，The autoimmune liver disease antibody repertoire detection kit that the present embodiment provides includes: external packing box 10，Reagent rack 20 and receiver 30 it is placed with in described external packing box 10，Described receiver 30 and reagent rack about 20 are placed in parallel，Described receiver 30 upper surface is provided with groove I 301，Description 31 is placed in described groove I 301，The left side of receiver 30 is provided with side channel I，Interpretation template 32 is positioned in described side channel I，The right side of receiver 30 is provided with side channel II，Accessory torr 33 is positioned in described side channel II，Described reagent rack 20 includes I-shaped reagent rack body 201，Described reagent rack body 201 be provided centrally with incubation slot 202，It is pasted with PVC liner plate 203 above described incubation slot 202，The upper surface of described PVC liner plate 203 divides two groups to be arranged side by side ten circles to hatch hole 204，Often group includes that five circles hatch hole 204，The upper surface of described reagent rack body 201 divides two groups to be arranged side by side six reagent putting reagent to put through hole，Often group includes that through hole put by three reagent，Concentrate buffer bottle 205、Enzyme conjugates bottle 206、Developer bottle 207、Sealer I bottle 208、Sealer II bottle 209 and sealer are placed in described reagent for III bottle 210 respectively and put in through hole.
In technique scheme, when using this autoimmune liver disease antibody repertoire detection kit, only the upper cover of external packing box 10 need to be opened, receiver 30 and reagent rack 20 are taken out from external packing box 10, then will interpretation template 32 and accessory torr 33 side channel I from receiver 30 and side channel II respectively be taken out, i.e. can be taken off the experimental tool that is placed in accessory torr 33 and be placed in the experiment reagent on receiver 30 and carry out test experience so that whole experimentation is convenient succinct.
With reference to shown in Fig. 3, the upper surface of described accessory torr 33 is provided with two parallel rectangular recess II 331 spaced apart, two groups of detection membrane bars 40 are respectively placed in described rectangular recess II 331, the side of described rectangular recess II 331 is provided with V-shaped groove 332, and tweezers 50 are positioned in described V-shaped groove 332.
With reference to shown in Fig. 1, described PVC liner plate 203 hides 4/5ths of incubation slot 202 at length direction, PVC liner plate 203 hides incubation slot 202 all at width, and PVC liner plate 203 can facilitate PVC liner plate 203 paste in incubation slot 202 and split at length direction slightly shorter than incubation slot 202.
In order to preferably explain this utility model, the operational approach of the special autoimmune liver disease antibody repertoire detection kit provided by this utility model is explained as follows:
(1) all reagent first balance to room temperature before using；
(2) the PVC liner plate 203 pasted on incubation slot 202 is taken off, use tweezers 50 to be pasted onto in incubation slot 202 by detection membrane bar 40, then cover PVC liner plate 203；
(3) in hatching hole 204, add concentration buffer, sealer, hatch (closing membrane bar)；
(4) sample to be tested, incubated at room temperature 30 minutes are added；
(5) with suction pipe, the reaction liquid hatched in hole 204 is thoroughly blotted, add buffer rinsing, after rinsing twice, the liquid hatched in hole 204 is blotted；
(6) in hatching hole 204, add buffer and enzyme conjugates, react 30 minutes under room temperature；
(7) with suction pipe, the reaction liquid hatched in hole 204 is thoroughly blotted, add buffer rinsing, after rinsing twice, the liquid hatched in hole 204 is blotted；
(8) in hatching hole 204, add distilled water and developer, react 15 minutes under room temperature；
(9) with suction pipe, the reaction liquid hatched in hole 204 is thoroughly blotted, add distilled water flushing once, repeat step 8 once；
(10) taking out detection membrane bar 40, contrast interpretation template 32 carries out result interpretation.
The foregoing is only preferred embodiment of the present utility model; not in order to limit this utility model; all within spirit of the present utility model and principle, done any modification, equivalent substitution and improvement etc., within should be included in protection domain of the present utility model.
Claims (3)
- null1. an autoimmune liver disease antibody repertoire detection kit，It is characterized in that including: external packing box (10)，Reagent rack (20) and receiver (30) it is placed with in described external packing box (10)，Described receiver (30) and reagent rack (20) left and right are placed in parallel，Described receiver (30) upper surface is provided with groove I (301)，Description (31) is placed in described groove I (301)，The left side of receiver (30) is provided with side channel I，Interpretation template (32) is positioned in described side channel I，The right side of receiver (30) is provided with side channel II，Accessory torr (33) is positioned in described side channel II，Described reagent rack (20) includes I-shaped reagent rack body (201)，Described reagent rack body (201) be provided centrally with incubation slot (202)，Described incubation slot (202) top is pasted with PVC liner plate (203)，The upper surface of described PVC liner plate (203) divides two groups to be arranged side by side ten circles to hatch hole (204)，Often group includes that five circles hatch hole (204)，The upper surface of described reagent rack body (201) divides two groups to be arranged side by side six reagent putting reagent to put through hole，Often group includes that through hole put by three reagent，Concentrate buffer bottle (205)、Enzyme conjugates bottle (206)、Developer bottle (207)、Sealer I bottle (208)、Sealer II bottle (209) and sealer III bottle (210) are placed in described reagent respectively and put in through hole.
- 2. autoimmune liver disease antibody repertoire detection kit as claimed in claim 1, it is characterized in that: the upper surface of described accessory torr (33) is provided with two parallel rectangular recess II (331) spaced apart, two groups of detection membrane bars (40) are respectively placed in described rectangular recess II (331), the side of described rectangular recess II (331) is provided with V-shaped groove (332), and tweezers (50) are positioned in described V-shaped groove (332).
- 3. autoimmune liver disease antibody repertoire detection kit as claimed in claim 2, it is characterized in that: described PVC liner plate (203) hides 4/5ths of incubation slot (202) at length direction, it is whole that PVC liner plate (203) hides incubation slot (202) at width.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620188279.5U CN205449993U (en) | 2016-03-11 | 2016-03-11 | Autoimmune liver disease antibody spectrum detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620188279.5U CN205449993U (en) | 2016-03-11 | 2016-03-11 | Autoimmune liver disease antibody spectrum detection kit |
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| Publication Number | Publication Date |
|---|---|
| CN205449993U true CN205449993U (en) | 2016-08-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620188279.5U Expired - Fee Related CN205449993U (en) | 2016-03-11 | 2016-03-11 | Autoimmune liver disease antibody spectrum detection kit |
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| Country | Link |
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| CN (1) | CN205449993U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107525924A (en) * | 2017-08-28 | 2017-12-29 | 张庆丰 | A kind of autoimmune hepatic diseases antibody assay kit |
-
2016
- 2016-03-11 CN CN201620188279.5U patent/CN205449993U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107525924A (en) * | 2017-08-28 | 2017-12-29 | 张庆丰 | A kind of autoimmune hepatic diseases antibody assay kit |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160810 Termination date: 20190311 |