CN201837629U - Indirect enzyme-linked immuno sorbent assay (ELISA) detection reagent kit for I type duck hepatitis virus antibodies - Google Patents
Indirect enzyme-linked immuno sorbent assay (ELISA) detection reagent kit for I type duck hepatitis virus antibodies Download PDFInfo
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- CN201837629U CN201837629U CN2010202989203U CN201020298920U CN201837629U CN 201837629 U CN201837629 U CN 201837629U CN 2010202989203 U CN2010202989203 U CN 2010202989203U CN 201020298920 U CN201020298920 U CN 201020298920U CN 201837629 U CN201837629 U CN 201837629U
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- elisa plate
- hepatitis virus
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Abstract
The utility model relates to an indirect enzyme-linked immuno sorbent assay (ELISA) detection reagent kit for I type duck hepatitis virus antibodies, which comprises a 96-hole ELISA plate, wherein the 96-hole ELISA plate consists of a plurality of ELISA plate units used for detecting the I type duck hepatitis virus antibodies, at least two holes are arranged on each ELISA plate unit, each ELISA plate unit can be provided with 12 holes, and the number of the ELISA plate units is eight. The 96-hole ELISA plate is the I type duck hepatitis virus antibody ELISA plate prepared by covering Duck Hepatitis Virus-I (DHV-I) capsid protein high antigen index region (VP1). The indirect ELISA detection reagent kit for the I type duck hepatitis virus antibodies also comprises 100* concentrated ELISA second antibody storage liquid, masculine reference liquid, feminine reference liquid, sample diluting liquid, 10* concentrated washing liquid, 100* concentrated substrate storage liquid, substrate buffer liquid and stop buffer, wherein the 100* concentrated ELISA second antibody storage liquid is hypothalamic regulatory peptide (HRP) marked goat anti rabbit (IgG) polyclonal antibody 100-time concentrated liquid. The indirect ELISA detection reagent kit adopts the dismountable 96-hole ELISA plate, and the resource waste can be reduced when the varieties of detection samples are few.
Description
Technical field
The utility model relates to a kind of antiviral antibody pick-up unit, particularly a kind of I type duck hepatitis virus antibody indirect ELISA (Enzyme-Linked Immunosorbnent Assay, enzyme connect immunosorbent and measure) detection kit.
Background technology
ELISA is that enzyme connects the abbreviation that immunosorbent is measured (Enzyme-Linked ImmunosorbnentAssay).ELISA is the laboratory diagnosis method of a kind of susceptibility height, high specificity, good reproducibility, because its stable reagent, easily preserve, easy and simple to handle, result judges factors such as more objective, and not only be suitable for extensive examination test but also can be used for the detection of a small amount of sample, both can do qualitative test and also can do advantages such as quantitative test, be widely used in fields such as microbiology, parasitology, oncology and cell factor.It is a kind of immunoenzyme technics that grows up after immunofluorescence and radioimmunoassay technique.This technology has developed very rapidly since coming out the beginning of the seventies, has been widely used in biology and medical science applied many fields at present.
ELISA is based on immunological response, the very high experimental technique of a kind of susceptibility that the specific reaction of antigen, antibody and enzyme are combined to the efficient catalytic effect of substrate.A kind of solid phase carrier that is reflected at owing to antigen, antibody---carry out in the hole of polystyrene microtiter plates, after a kind of reagent of every adding is hatched, can remove unnecessary free reactant by washing, thus warranty test result's specificity and stability.In actual applications, by different designs, concrete method step can have multiple.That is: be used to detect antibody indirect method, be used to detect the double antibody sandwich method of antigen and be used to detect micromolecule antigen or haptenic antigenic competition method or the like.Relatively more commonly used is ELISA double antibody sandwich method and ELISA indirect method.
The kit that is used for the detection of ELISA indirect method is called indirect ELISA testing kit, at present, carry out the detection of I type duck hepatitis virus antibody indirect ELISA and need one 96 hole ELISA Plate at least, when sample type more after a little while, untapped hole count is more in the ELISA Plate, but this plate can not be reused again, can cause the wasting of resources.
The utility model content
The purpose of this utility model is to provide a kind of I type duck hepatitis virus antibody indirect ELISA testing kit.96 hole ELISA Plate can not be split in the prior art to solve, when the test sample kind more after a little while, can cause the technical matters of the wasting of resources.
A kind of I type duck hepatitis virus antibody indirect ELISA testing kit comprises 96 hole ELISA Plate, and described 96 hole ELISA Plate are made up of in order to the ELISA Plate unit that detects I type duck hepatitis virus antibody some, and at least 2 above hole counts are set on each ELISA Plate unit.
Preferably, described each ELISA Plate unit is set to 12 holes, and described ELISA Plate unit is 8.
Wherein, described 96 hole ELISA Plate are for wrapping the I type duck hepatitis virus antibody ELISA Plate that is made by DHV-I capsid protein high antigenic index region (VP1).
Described I type duck hepatitis virus antibody indirect ELISA testing kit also comprises: 100* concentrates ELIAS secondary antibody stock solution, positive control solution, negative controls, sample diluting liquid, 10* concentrated cleaning solution, 100* concentrated substrate stock solution, substrate buffer solution and stop buffer, and it is 100 times of concentrates of HRP mark goat anti-rabbit igg polyclonal antibody that described 100* concentrates the ELIAS secondary antibody stock solution.
The utility model is 96 detachable hole ELISA Plate, when the test sample kind more after a little while, can reduce the wasting of resources.
Description of drawings
Fig. 1 is the structural representation of I type duck hepatitis virus antibody indirect ELISA testing kit.
Fig. 2 is the structural representation of each ELISA Plate unit in the I type duck hepatitis virus antibody indirect ELISA testing kit.
Fig. 3 is the enlarged drawing of B1 among Fig. 2.
Embodiment
Below in conjunction with accompanying drawing, the utility model is done further detailed narration.Should be understood that these embodiment only to be used to the utility model is described and be not used in the restriction scope of the present utility model.
See also Fig. 1, Fig. 2, Fig. 3, I type duck hepatitis virus antibody indirect ELISA testing kit A, comprise 96 hole ELISA Plate B, 100* concentrates ELIAS secondary antibody stock solution 1, positive control solution 2, negative controls 3, sample diluting liquid 4,10* concentrated cleaning solution 5,100* concentrates substrate stock solution 6, substrate buffer solution 7 and stop buffer 8, Fig. 3 is the structural representation of each ELISA Plate unit, each ELISA Plate unit is set to 12 holes, and 10 for wrapping the I type duck hepatitis virus antibody enzyme mark hole that is made by DHV-I capsid protein high antigenic index region (VP1), can wrap by different viral antigens on different ELISA Plate unit.And 96 hole ELISA Plate B are positioned in the detection kit A.
When the sample kind that detects when need is less than 10 kinds, only need takes out a plate (12 hole) and detect and get final product.
Bag can be carried out the detection of different antiviral antibodies by different virus antigen on different ELISA Plate.
Embodiment 1, bag can be made DHV-I antibody 12 hole ELISA Plate by DHV-I capsid protein high antigenic index region (VP1) on 12 hole ELISA Plate 1, it is 100 times of concentrates of HRP mark goat anti-rabbit igg polyclonal antibody that 100* concentrates ELIAS secondary antibody stock solution 1, positive control solution is the positive duck serum dilution of DHV-I, and negative controls 4 is the negative duck serum dilution of DHV-I.This kit can be used for detecting DHV-I antibody, and following good effect is arranged: detection kit is easy to apply on a large scale to high specificity, the susceptibility height, simple to operate of DHV-I antibody, has vast market prospect.
More than disclosed only be several specific embodiments of the application, but the application is not limited thereto, any those skilled in the art can think variation, all should drop in the application's the protection domain.
Claims (3)
1. I type duck hepatitis virus antibody indirect ELISA testing kit, comprise 96 hole ELISA Plate, it is characterized in that described 96 hole ELISA Plate are made up of in order to the ELISA Plate unit that detects I type duck hepatitis virus antibody some, and at least 2 above hole counts are set on each ELISA Plate unit.
2. I type duck hepatitis virus antibody indirect ELISA testing kit as claimed in claim 1 is characterized in that each ELISA Plate unit is set to 12 holes, and described ELISA Plate unit is 8.
3. I type duck hepatitis virus antibody indirect ELISA testing kit as claimed in claim 1 is characterized in that, the I type duck hepatitis virus antibody ELISA Plate that 96 hole ELISA Plate are made by DHV-I capsid protein high antigenic index region for bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010202989203U CN201837629U (en) | 2010-08-19 | 2010-08-19 | Indirect enzyme-linked immuno sorbent assay (ELISA) detection reagent kit for I type duck hepatitis virus antibodies |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010202989203U CN201837629U (en) | 2010-08-19 | 2010-08-19 | Indirect enzyme-linked immuno sorbent assay (ELISA) detection reagent kit for I type duck hepatitis virus antibodies |
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| CN201837629U true CN201837629U (en) | 2011-05-18 |
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| CN2010202989203U Expired - Fee Related CN201837629U (en) | 2010-08-19 | 2010-08-19 | Indirect enzyme-linked immuno sorbent assay (ELISA) detection reagent kit for I type duck hepatitis virus antibodies |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102344973A (en) * | 2011-11-11 | 2012-02-08 | 广西壮族自治区兽医研究所 | Duck hepatitis virus type I LAMP (loop-mediated isothermal amplification) detection kit |
| CN104297493A (en) * | 2014-10-31 | 2015-01-21 | 四川农业大学 | Application of soluble I-type duck hepatitis virus 3D protein to preparation of ELISA reagent and ELISA kit |
| CN104764887A (en) * | 2015-04-29 | 2015-07-08 | 山东畜牧兽医职业学院 | Competitive ELISA detection method for DHAV-1 antibodies |
| CN106370854A (en) * | 2016-08-17 | 2017-02-01 | 中国科学院微生物研究所 | Duck hepatitis A virus 1 antibody detection method and special-purpose kit thereof |
-
2010
- 2010-08-19 CN CN2010202989203U patent/CN201837629U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102344973A (en) * | 2011-11-11 | 2012-02-08 | 广西壮族自治区兽医研究所 | Duck hepatitis virus type I LAMP (loop-mediated isothermal amplification) detection kit |
| CN102344973B (en) * | 2011-11-11 | 2013-04-24 | 广西壮族自治区兽医研究所 | Duck hepatitis virus type I LAMP (loop-mediated isothermal amplification) detection kit |
| CN104297493A (en) * | 2014-10-31 | 2015-01-21 | 四川农业大学 | Application of soluble I-type duck hepatitis virus 3D protein to preparation of ELISA reagent and ELISA kit |
| CN104764887A (en) * | 2015-04-29 | 2015-07-08 | 山东畜牧兽医职业学院 | Competitive ELISA detection method for DHAV-1 antibodies |
| CN104764887B (en) * | 2015-04-29 | 2018-08-17 | 山东畜牧兽医职业学院 | A kind of 1 type duck hepatitis A virus antibody competition ELISA detection method |
| CN106370854A (en) * | 2016-08-17 | 2017-02-01 | 中国科学院微生物研究所 | Duck hepatitis A virus 1 antibody detection method and special-purpose kit thereof |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110518 Termination date: 20130819 |