CN203324272U

CN203324272U - Solid phase membrane detection reagent reaction tank

Info

Publication number: CN203324272U
Application number: CN201320344024XU
Authority: CN
Inventors: 吴善东
Original assignee: Hangzhou Zheda Dixun Biological Gene Engineering Co Ltd
Current assignee: Hangzhou Zheda Dixun Biological Gene Engineering Co Ltd
Priority date: 2013-06-14
Filing date: 2013-06-14
Publication date: 2013-12-04
Anticipated expiration: 2023-06-14

Abstract

The utility model discloses a solid phase membrane detection reagent reaction tank. The solid phase membrane detection reagent reaction tank comprises a tank body and a handle, wherein one side of the tank body is provided with a bayonet which can be adaptive to a clamping slot in a reaction tank support, and the handle is arranged at the other side of the tank body. The reaction tank provided by the utility model can be inserted in and drawn out of the reaction tank support easily, eliminates the hazard for polluting a membrane surface during the insertion and drawing process, increases an area for labeling specimen information, and can be applied to the design of western blots and protein chip detection reagents.

Description

Immobilon-p detects the reagent reacting groove

Technical field

The utility model relates to a kind of immobilon-p and detects the reagent reacting groove.

Background technology

At present, the most of solid film detects the reagent reacting groove because reaction, wash liquid is toppled over and changes inconvenient, needs to coordinate self-reacting device to use, and cost is higher, is not suitable for the basic medical unit use.The Western blotting of Germany Mediwiss-analytic company invention detects reagent reacting groove complex reaction groove support and uses, can realize easily reagent washing, change the manual operations of the steps such as liquid, can realize that single part of detection or many person-portions detect simultaneously, are applicable to scope wide.But the reactive tank of German Mediwiss-analytic company is being installed to that the reactive tank support gets on and when support takes out, finger need to be held the action that plug is realized in the reactive tank both sides, because lifting surface area is less, comparatively painstaking during plug, and point the surface that easily touches membrane reagent in reactive tank in the plug process, cause background contamination.In addition, German Mediwiss-analytic company reactive tank only can simply mark sample number into spectrum at reactive tank bayonet socket place, can't mark more sample information.

Summary of the invention

Detect reactive tank that the reagent reacting groove exists and plug on the reactive tank support and relatively require great effort, face easily polluted and mark the shortcomings such as sample information area is little in the plug process in order to overcome existing immobilon-p, the utility model discloses a kind of immobilon-p detection reagent reacting groove.

The technical solution adopted in the utility model is:

Immobilon-p detects the reagent reacting groove, comprises cell body, described cell body one side be provided with can with the reactive tank support on the suitable bayonet socket of draw-in groove, it is characterized in that: also comprise handle, described handle is arranged on the opposite side of described cell body.

Further, described handle is shaped as rectangle, square, semicircle, ellipse, fan-shaped, trapezoidal, triangle or other geometric configuratioies.

Further, described cell body is shaped as rectangle.

Further, described reactive tank is made of plastics.

When the utility model is used, operator one holds the reactive tank support, and one holds the handle place of reactive tank, reactive tank easily can be installed on support or from support and take off.In experimentation, the handle place can mark sample information simultaneously.

The beneficial effects of the utility model are embodied in: this use novel reaction groove can plug like a cork on the reactive tank support, and has eliminated the risk in the plug process, face polluted, and has increased the area for mark sample information simultaneously; Can be applicable to the design of Western blotting, protein chip detection reagent.

The accompanying drawing explanation

Fig. 1 is the utility model front view.

Fig. 2 is the utility model vertical view.

Fig. 3 is the utility model upward view.

Embodiment

Referring to figs. 1 through Fig. 3, immobilon-p detects the reagent reacting groove, comprises cell body 1, described cell body 1 one sides be provided with can with the reactive tank support on the suitable bayonet socket 2 of draw-in groove, also comprise handle 3, described handle 3 is arranged on the opposite side of described cell body 1.

Further, described handle 3 is shaped as rectangle, square, semicircle, ellipse, fan-shaped, trapezoidal, triangle or other geometric configuratioies.

Further, described cell body 1 is shaped as rectangle.

Further, described reactive tank is made of plastics.

When the utility model is used, operator one holds the reactive tank support, and one holds handle 3 places of reactive tank, reactive tank easily can be installed on support or from support and take off.In experimentation, the handle place can mark sample information simultaneously.

When the utility model is specifically related to the detection of allergen specificity antibody IgE Western blotting, concrete experimental procedure is as follows:

1, the detection reagent membrane bar of allergen specificity antibody IgE Western blotting is cut into to the shape of described cell body, paste into described cell body bottom, be made into the reaction reagent bar, sample information on the handle place marks simultaneously; One holds the reactive tank support, and one holds the handle place of reactive tank, the bayonet socket of reactive tank is snapped in the draw-in groove of reactive tank support; Can snap at most 10 reactive tanks (i.e. 10 person-portion reagent) on each reactive tank support simultaneously.

2, after adding cleansing solution to make film bar reagent fully wetting, pick up the reactive tank support on the other hand in rack-mount reactive tank, the cleansing solution in reactive tank is outwelled to rear drying.

3, add the corresponding serum specimen of 250-300 microlitre in each reactive tank, support is placed on vortex mixer to the room temperature blending incubation after 45 minutes together with reactive tank, pick up on the other hand the reactive tank support, sample in reactive tank is carefully toppled over and along cell body, then with the cleansing solution in wash bottle, rinse back and forth face and 5 dryings afterwards of reaction cell body.

4, add 250-300 microlitre two hole working fluids in each reactive tank, support is placed on vortex mixer to the room temperature blending incubation after 45 minutes together with reactive tank, pick up on the other hand the reactive tank support, liquid runs down cell body in reactive tank is carefully toppled over and, then with the cleansing solution in wash bottle, rinse back and forth face and 5 dryings afterwards of reaction cell body.

5, add 250-300 microlitre enzyme working fluid in each reactive tank, support is placed on vortex mixer to the room temperature blending incubation after 20 minutes together with reactive tank, pick up on the other hand the reactive tank support, liquid runs down cell body in reactive tank is carefully toppled over and, then with the cleansing solution in wash bottle, rinse back and forth face and 5 dryings afterwards of reaction cell body.

6, add 250-300 microlitre substrate solution in each reactive tank, support is placed on vortex mixer to the room temperature blending incubation after 20 minutes together with reactive tank, on the other hand pick up the reactive tank support, the liquid runs down cell body in reactive tank is toppled over, so and with tap water, face and reactive tank are rinsed well.

7, after all faces and reactive tank are dried up, one holds the reactive tank support, and one holds the handle place of reactive tank, and reactive tank is extracted from the reactive tank support, proceeds to supporting interpretoscope sentence read result.

The described content of this instructions embodiment is only enumerating the way of realization of inventive concept; protection domain of the present utility model should not be regarded as only limiting to the concrete form that embodiment states, protection domain of the present utility model also reaches the equivalent technologies means that design can be expected according to the utility model in those skilled in the art.

Claims

1. immobilon-p detects the reagent reacting groove, comprises cell body, described cell body one side be provided with can with the reactive tank support on the suitable bayonet socket of draw-in groove, it is characterized in that: also comprise handle, described handle is arranged on the opposite side of described cell body.

2. immobilon-p as claimed in claim 1 detects the reagent reacting groove, it is characterized in that: described handle be shaped as rectangle, square, semicircle, ellipse, fan-shaped, trapezoidal, triangle or other geometric configuratioies.

3. immobilon-p as claimed in claim 2 detects the reagent reacting groove, it is characterized in that: described cell body be shaped as rectangle.

4. immobilon-p as claimed in claim 3 detects the reagent reacting groove, and it is characterized in that: described reactive tank is made of plastics.