CN201518026U

CN201518026U - Lymphocyte human leukocyte antigen B5 solid-phase detecting reagent case

Info

Publication number: CN201518026U
Application number: CN2009202208761U
Authority: CN
Inventors: 傅金瑞; 王高升; 李立和
Original assignee: Tianjin Baodi Hospital
Current assignee: Tianjin Baodi Hospital
Priority date: 2009-09-25
Filing date: 2009-09-25
Publication date: 2010-06-30
Anticipated expiration: 2019-09-25

Abstract

The utility model relates to a lymphocyte human leukocyte antigen B5 solid-phase detecting reagent case, comprising a case body and a case cover connected onto the case body. A cover glass box and a slide glass box are fixed side by side at the left end inside the case body, two lines of reagent bottles are placed at the other positions inside the case body, and are all provided with bottle caps, sponge is arranged at the bottom of the case body, and is provided with a hole for positioning, and the diameter of the hole is the same as those of the longitudinal cross sections of the cover glass box and the slide glass box and the reagent bottles. A square groove is arranged on the left half portion of the slide glass, and has the dimension of 3mm, 3mm and 1mm, positive cells are fixed on the bottom surface of the groove, and a plane smear is arranged on the right half portion of the slide glass to contain lymphocytes to be detected. The lymphocyte human leukocyte antigen B5 solid-phase detecting reagent case has the advantages of convenient carrying, simple operation, zero fault operation, less labour and time consumption and short detecting time about 2 hours because the reagent for detecting, the slide glass and the cover glass both with positive control are gathered together.

Description

Lymphocyte humam leucocyte antigen B5 solid phase detection kit

Technical field

The utility model relates to a kind of kit, particularly relates to the solid phase detection kit of a kind of lymphocyte humam leucocyte antigen B5.

Background technology

The Behcet's disease clinical manifestation is very complicated, be difficult to diagnosis, humam leucocyte antigen B5 is considered to this sick heredity mark, help this sick examination and make a definite diagnosis, present domestic widespread use be microlymphocytotoxicity test, it belongs to liquid phase process, needs the cell effect plate, micro syringe, specific installations such as inverted microscope, trivial operations, (going out the result about 12 hours) consuming time, testing result is vulnerable to the complement quality, the reaction plate quality is extracted cell concentration, influence of various factors such as cell survival, repeatability is relatively poor, need the average of a peek parallel testing result to mend this deficiency, thereby increase the detection cost.

Summary of the invention

The utility model provides a kind of solid phase detection method time and labour saving economical with materials again that is used for for solving the technical matters that exists in the known technology, and testing result accurately and the solid phase detection kit of the low lymphocyte humam leucocyte antigen B5 of cost.

The technical scheme that the utility model is taked for the technical matters that exists in the solution known technology is, it comprises box body and lid, it is characterized in that: the box body top is connected with lid, left end in the box body is fixed cap slide box and Glass carrier box side by side, two row's reagent bottles are laid at other position in the box body, reagent bottle all has bottle cap, bottom at box body is placed with sponge, have on the sponge and the cover glass box, the hole that the Glass carrier box longitudinal cross-section is identical with the reagent bottle diameter, the left-half of described microslide is manufactured with square groove, the lower surface of groove is fixed with positive cell, and the right half part of microslide is the smear on plane.

The utility model can also be by the following technical solutions:

Described groove is square or circular, and its square groove is of a size of 3 * 3 * 1mm, and its circular groove is of a size of

3 * 1mm;

The material of described box body, lid, cover glass box, Glass carrier box is rigid plastic;

Advantage and the good effect that the utlity model has are: reagent, cover glass and the microslide that uses when detecting owing to all (attaching has positive control) all concentrates in together, be easy to carry, simple to operate, be difficult for during use making a mistake, also need not now join, saved manpower and time and reagent, shorten detection time, can finish experiment in general about 2 hours.

Description of drawings

Fig. 1 is the utility model one-piece construction synoptic diagram;

Fig. 2 is the slide configurations synoptic diagram;

Fig. 3 is the sponge structure synoptic diagram.

Among Fig. 1-Fig. 3: 1. box body, 2. lid, 3. cover glass box, 4. Glass carrier box, 5 reagent bottles, 6. bottle cap, 7. sponge, 8. microslide, 9. groove, 10. smear.

Embodiment

For further understanding summary of the invention of the present utility model, characteristics and effect, exemplify following examples now, and conjunction with figs. is described in detail as follows, see also Fig. 1-Fig. 3.

As shown in Figure 1-Figure 3: box body 1 top is connected with lid 2, left end in the box body 1 is fixed cap slide box 3 and Glass carrier box 4 side by side, two row's reagent bottles 5 are laid at other position in the box body 1, reagent bottle 5 all has bottle cap 6, be placed with sponge 7 in the bottom of box body 1, have on the sponge 7 and cover glass box 3 and Glass carrier box 4 longitudinal cross-sections and the identical hole of reagent bottle 5 diameters, the hole on the sponge 7 in order to the location, microslide 8 left-half be manufactured with square groove 9 (or circular groove its be of a size of 3 * 1mm), the size of groove 9 is 3 * 3 * 1mm, and the lower surface of groove 9 is fixed with positive cell, and the right half part of microslide 8 is the institute of tested lymphocyte smear 10.The material of described box body 1, lid 2, cover glass box 3, Glass carrier box 4 is rigid plastic.

During use, be that the application cell separating agent prepares the lymphocyte suspension, smear is dry fixing, drip antibody A respectively on smear and in the groove, temperature is bathed the flushing back and is dripped antibody B, temperature is bathed the flushing back and is dripped fluorescer, and temperature is bathed flushing back mountant and cover plate mounting, judges smear results with reference to the groove positive control again under fluorescent microscope.

Its advantage is: reagent, cover glass and the slide (being accompanied with positive control) that use when detecting owing to all concentrate in together, be easy to carry, simple to operate, need not on-the-spot preparation also be difficult for mistaken during use, save manpower and time, be about 2 hours detection time, saved reagent, inspection cost reduces, and is about 2/3 of microlymphocyto toxicity liquid phase method material.

Claims

1. lymphocyte humam leucocyte antigen B5 solid phase detection kit, it comprises box body and lid, it is characterized in that: box body (1) top is connected with lid (2), left end in the box body (1) is fixed cap slide box (3) and Glass carrier box (4) side by side, two row's reagent bottles (5) are laid at other position in the box body (1), reagent bottle (5) all has bottle cap (6), be placed with sponge (7) in the bottom of box body (1), have on the sponge (7) and cover glass box (3), Glass carrier box (4) longitudinal cross-section and the identical hole of reagent bottle (5) diameter, the left-half of described microslide (8) is manufactured with square groove (9), the lower surface of groove (9) is fixed with positive cell, and the right half part of microslide (8) is the smear (10) on plane.

2. lymphocyte humam leucocyte antigen B5 solid phase detection kit according to claim 1, it is characterized in that: described groove (9) is square or circular, and its square groove is of a size of 3 * 3 * 1mm,, its circular groove is of a size of

3 * 1mm.

3. lymphocyte humam leucocyte antigen B5 solid phase detection kit according to claim 1, it is characterized in that: the material of described box body (1), lid (2), cover glass box (3), Glass carrier box (4) is rigid plastic.