CN202369594U - Glossy ganoderma cell incubator - Google Patents

Glossy ganoderma cell incubator Download PDF

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Publication number
CN202369594U
CN202369594U CN 201120545845 CN201120545845U CN202369594U CN 202369594 U CN202369594 U CN 202369594U CN 201120545845 CN201120545845 CN 201120545845 CN 201120545845 U CN201120545845 U CN 201120545845U CN 202369594 U CN202369594 U CN 202369594U
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CN
China
Prior art keywords
bottle
cell
glossy ganoderma
incubator
cell bottle
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Expired - Fee Related
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CN 201120545845
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Chinese (zh)
Inventor
岳才军
王艳红
王忠乔
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Heilongjiang Bayi Agricultural University
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Heilongjiang Bayi Agricultural University
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Priority to CN 201120545845 priority Critical patent/CN202369594U/en
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Publication of CN202369594U publication Critical patent/CN202369594U/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/08Flask, bottle or test tube
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/02Filters

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model relates to a glossy ganoderma cell incubator. The glossy ganoderma cell incubator comprises an electromagnetic stirring instrument and a cell bottle; the cell bottle is a conical flask; the cell bottle is placed on the electromagnetic stirring instrument; a bacteria filter membrane is arranged at the mouth of the cell bottle; the bacteria filter membrane is fastened at the bottle mouth through a bottle mouth hoop; a bottle cap is in threaded connection with the bottle mouth hoop and provided with a pore; and an air supply tube is inserted into the cell bottle from the bottle cap, and a water supply tube is arranged on a bottle body of the cell bottle; and a magnetic stirring bar is arranged in the cell bottle. The glossy ganoderma cell incubator is easy to operate, easy to sterilize and difficult to pollute, does not require additional sterile ventilation, has variable culture volume, and can achieve a good glossy ganoderma cell culture effect.

Description

A kind of glossy ganoderma cell incubator
Technical field:
The utility model relates to the container of cultivating glossy ganoderma cell, what be specifically related to is a kind of glossy ganoderma cell incubator.
Background technology:
Glossy ganoderma is precious Chinese medicinal materials, and Ganodenic acid and ganoderan are the basic substances of glossy ganoderma physiologically active.Can obtain Ganodenic acid and ganoderan now through external liquid culture glossy ganoderma cell.Glossy ganoderma cell can shake bottle shaking culture on shaking table with the microorganism fermentation tank cultivation or with common triangle.But microorganism fermentation tank costs an arm and a leg and complicated operation, and sterile air need be provided.Use the shaking table shaking culture, cultivate small scale (the maximum triangular flask that uses on the shaking table is 500ml, and volume greatly just is difficult to vibration again), nutrient and moisture are used up easily and are scattered and disappeared.
Summary of the invention:
The purpose of the utility model provides a kind of glossy ganoderma cell incubator, and it is used to solve unhandy problem when having the container culturing cell of cultivating glossy ganoderma cell now.
The utility model solves the technical scheme that its technical problem adopted: this glossy ganoderma cell incubator comprises induction stirring appearance and cell bottle; The cell bottle is an Erlenmeyer flask; The cell bottle is placed on the induction stirring appearance, and the bottleneck of cell bottle is provided with the bacterium filtering membrane, and the bacterium filtering membrane is fastened on bottle mouth position through the bottleneck hoop; Bottle cap is threaded with the bottleneck hoop, and bottle cap has pore; Blowdown pipe is inserted in the cell bottle from bottle cap, on the cell bottle bottle filling pipe is set; Stirring rod is magnetic in the cell bottle.
The cell bottle is processed with transparent material in the such scheme.
Blowdown pipe is made of plastic in the such scheme.
The utlity model has following beneficial effect:
1, the utility model is simple to operate, is easy to sterilization, is difficult for polluting, and does not need the aseptic ventilation of extra confession, and volume of culture is changeable, can reach good glossy ganoderma cell culture effect.
2, the utility model is simple in structure, and cost is low, is suitable for medium and small sized enterprises and laboratory and uses.
Description of drawings
Fig. 1 is the structural representation of the utility model.
1. induction stirring appearance, 2. cell bottle, 3. bottle cap, 4. pore, 5. blowdown pipe, 6 magnetic stirring bars, 7 operating panels, 8 power switches, 9 filling pipes.
Embodiment
Below in conjunction with accompanying drawing the utility model is done further explanation:
As shown in Figure 1, this glossy ganoderma cell incubator comprises induction stirring appearance 1 and cell bottle 2, and cell bottle 2 is placed on the induction stirring appearance 1; Cell bottle 2 is Erlenmeyer flasks, processes with transparent material, and the bottleneck of cell bottle 2 is provided with the bacterium filtering membrane; The bacterium filtering membrane is fastened on bottle mouth position through the bottleneck hoop; The bottleneck hoop is the little cylinder of both ends open, and screw thread is all arranged on the inwall of bottleneck hoop and the outer wall, and bottle cap 3 is threaded with the bottleneck hoop; Bottle cap has pore 4, and blowdown pipe 5 is inserted in the cell bottle 2 from bottle cap 3, and blowdown pipe 5 is made of plastic; The top of cell bottle 2 bottles is connected to a filling pipe 9, can in the cell bottle, replenish the moisture that is scattered and disappeared in the culturing process through this filling pipe 9, also can add nutrient or stimulator.The stirring rod 6 that is magnetic in the induction stirring appearance 1 has operating panel 7 and power switch 8 on the shell of induction stirring appearance 1.
Cell bottle 2 is transparent, is convenient to observe the growing state of bottle inner cell like this, and pore 4 on the bottle cap 3 and bacterium filtering membrane can make things convenient for the turnover of air, can stop the entering of mikrobe again simultaneously, so the utility model need not extraly provide sterile air.In addition; Blowdown pipe 5 on the cell bottle cap 3 is the inlets that add gases such as oxygen, carbonic acid gas, ethene to the cell bottle; The operation that convenient research uses these gases to improve chemical substances such as Ganodenic acid, when not adding these gases, this blowdown pipe 5 can play the effect of gas air exchange equally.Also have, cell bottle 2 can independently place the high pressure moist heat sterilizer to sterilize, and guarantees sterilising effect easily.
Induction stirring appearance 1 in the utility model stirs appearance for great-power electromagnetic; Elongated magnetic stirring bar 6 places in the cell bottle 2; Its length is slightly less than cell bottle 2 internal diameters, under the driving of great-power electromagnetic stirring appearance 1, can rotate, and drive glossy ganoderma cell and culture medium movement in the cell bottle 2; Good mass transfer effect is arranged, guaranteed the growth of glossy ganoderma cell and the needs of synthetic nutrition of Ganodenic acid isoreactivity material and microenvironment.Owing to adopted powerful induction stirring appearance 1 that the cultivation scale of single glossy ganoderma cell incubator is reached more than the 20000ml.Powerful induction stirring appearance 1 mainly contains power switch 8 and speed governing button, and the speed governing button is arranged on the operating panel 7, such as, volume of culture is when 2000ml, and rotating speed is controlled at about 300 rev/mins and gets final product, so simple to operate.

Claims (3)

1. glossy ganoderma cell incubator; It is characterized in that: this glossy ganoderma cell incubator comprises induction stirring appearance (1) and cell bottle (2), and cell bottle (2) is an Erlenmeyer flask, and cell bottle (2) is placed on the induction stirring appearance (1); The bottleneck of cell bottle (2) is provided with the bacterium filtering membrane; The bacterium filtering membrane is fastened on bottle mouth position through the bottleneck hoop, and bottle cap (3) is threaded with the bottleneck hoop, and bottle cap (3) has pore (4); Blowdown pipe (5) is inserted in the cell bottle (2) from bottle cap (3), on cell bottle (2) bottle filling pipe (9) is set; The stirring rod that is magnetic in the cell bottle (2) (6).
2. glossy ganoderma cell incubator according to claim 1 is characterized in that: described cell bottle (2) is processed with transparent material.
3. glossy ganoderma cell incubator according to claim 1 is characterized in that: described blowdown pipe (5) is made of plastic.
CN 201120545845 2011-12-23 2011-12-23 Glossy ganoderma cell incubator Expired - Fee Related CN202369594U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201120545845 CN202369594U (en) 2011-12-23 2011-12-23 Glossy ganoderma cell incubator

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201120545845 CN202369594U (en) 2011-12-23 2011-12-23 Glossy ganoderma cell incubator

Publications (1)

Publication Number Publication Date
CN202369594U true CN202369594U (en) 2012-08-08

Family

ID=46593307

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 201120545845 Expired - Fee Related CN202369594U (en) 2011-12-23 2011-12-23 Glossy ganoderma cell incubator

Country Status (1)

Country Link
CN (1) CN202369594U (en)

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C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20120808

Termination date: 20121223