CN201354373Y - Rolling load device for constructing cartilage in vitro - Google Patents
Rolling load device for constructing cartilage in vitro Download PDFInfo
- Publication number
- CN201354373Y CN201354373Y CNU2008201439849U CN200820143984U CN201354373Y CN 201354373 Y CN201354373 Y CN 201354373Y CN U2008201439849 U CNU2008201439849 U CN U2008201439849U CN 200820143984 U CN200820143984 U CN 200820143984U CN 201354373 Y CN201354373 Y CN 201354373Y
- Authority
- CN
- China
- Prior art keywords
- wedge block
- compression adjustment
- cartilage
- roller
- adjustment frame
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a rolling load device for constructing cartilage in vitro according to the simulation principle of tissue engineering construction, which comprises a rolling control mechanism and a compression adjusting mechanism, wherein the rolling control mechanism is used for exerting the mechanical condition of rolling load on culture. On the one hand, the device can control the rolling speed of a roller through a stepper motor and a leading screw, and a roller which is formed by gears and racks rolls purely along the culture at even speed; on the other hand, the working surface can be horizontally lifted through the relative sliding of wedge-shaped sliding blocks, thereby, the even and adjustable amount of compression can be generated on the culture. The device simulates the movement state of knee joint, and the rolling load is similar to the actual stress state of the cartilage, and therefore, the research model of mechanical effects of artificial cartilage can be formed under the rolling load conditions, thereby being conducive to the cartilage exploration in cartilage mechanics biology.
Description
Technical field
The present invention relates to cell engineering and field of tissue engineering technology, particularly relate to a kind of roll extrusion deceleration loading device that is used for the cartilage external structure.
Background technology
In recent years, because cartilage degradation or damagedly cause arthralgia, moving obstacle even completely lose the difficult problem that function of joint has become therapeutic treatment.Although adopt a lot of new technologies, novel method to treat clinically, do not obtain satisfactory therapeutic effects but still form suitable method.
At present, cartilage tissue engineeredly just becoming the damaged Perfected process of a kind of permanent repair tissue, and all obtaining huge progress at aspects such as seed cell, timbering material, growth regulator, tissue constructions.Current normally used pair cell is cultivated, tissue and cell-scaffold culture are cultivated and the bio-reactor of maintenance has four kinds, that is: static bio-reactor, rotating and culturing bottle bio-reactor, rotating wall vessel bioreactor and perfusion type bio-reactor [Advanced Drug Delivery Reviews 33 (1998) 15-30, Tissue Engineering.2003,9 (3), 549-553], different seed cells, the New-support material, somatomedins etc. constitute culture, cultivating in these several bio-reactors respectively, mainly is to be beneficial to the culture form, the growth of structure.Although the growth of culture has been optimized in the use of various bio-reactors in the prior art, the growth of culture, growth still do not reach clinical requirement.The artificial cartilage of cultivating also has bigger gap with the live body cartilage on 26S Proteasome Structure and Function, this has directly influenced artificial cartilage application clinically.
In fact have a lot of physical factors all can influence the g and D of cartilaginous tissue, and mechanics factor has played main effect therein.Therefore, Many researchers has been developed the multiple bio-reactor that mechanical environment is arranged, attempt cultivating the functionalization artificial cartilage, the mechanical condition of these reactors has: fluid shearing stress, liquid pressure, stretching, directly compression, distortion shearing stress or the wherein combination of one or more conditions.Wherein the condition of the cultivation cartilage of fluid shearing stress, liquid pressure and direct compression just is widely studied.But there is the function of support, motion in the joint, and synovia, nutrition cartilage and lubricated cartilage surface are arranged between distal femur and the proximal tibia.Distal femur and proximal tibia are in contact with one another, and two kinds of relative movement are arranged, and a kind of is to roll, and another kind is to slide, and can vividly describe the direct contact compression into a kind of cylinder, and another kind of seemingly wheel slides, and produces surperficial shearing stress.Rolling produces compression to cartilage on the one hand, also causes shearing strain on the other hand.Roll extrusion load is the main representative of live body joint cartilage mechanical environment.For organizational project, different tissues has different mechanical conditions, and this through engineering approaches of joint cartilage makes up just needs specific mechanical environment.According to the emulation principle that organizational project makes up, roll extrusion loads the structure that is more suitable for joint cartilage.
The utility model content
The technical problems to be solved in the utility model provides the roll extrusion deceleration loading device that is used for the cartilage external structure that a kind of emulation principle that makes up according to organizational project designs.
The technical scheme of the roll extrusion deceleration loading device that is used for the cartilage external structure of the present utility model is as follows:
A kind of roll extrusion deceleration loading device that is used for the cartilage external structure comprises the compression adjustment mechanism and the rolling controlling organization that is used for culture is applied roll extrusion load that are used to regulate to the draught of culture.
Above-mentioned compression adjustment mechanism comprises: the compression adjustment frame, platform, adjusting screw(rod), the 1st wedge block, the 2nd wedge block, pressing plate, culturing room, underframe and return spring, wherein, the compression adjustment frame has the cross section that is similar to U-shaped, two side arm passes two rectangular openings that form on the platform respectively, below platform, extend upwardly to above the platform, the inboard, upper end of two side arms of compression adjustment frame is set with a pressing plate respectively, in underframe, be provided with the 1st wedge block and the 2nd wedge block, a pair of adjusting screw(rod) is fixed on the both sides of underframe, two adjusting screw(rod)s are connected with an end of the 1st wedge block and the 2nd wedge block respectively, make it produce relative movement, the top of the bottom surface of compression adjustment frame is connected with the bottom surface of platform by return spring, but the lower surface of compression adjustment frame contacts with the upper surface of the 1st wedge block and relatively sliding, the upper surface of the 1st wedge block, the lower surface of compression adjustment frame is all parallel with platform, but the inclined-plane that is positioned at the bottom of the 1st wedge block overlaps with the superposed inclined-plane of the 2nd wedge block and relatively sliding.
Above-mentioned rolling controlling organization comprises stepper-motor, motor shaft, leading screw, web member, connecting rod, roller, gear and tooth bar, described stepper-motor is connected with leading screw by motor shaft, the other end of leading screw is connected with connecting rod by web member, the end of connecting rod is provided with draw-in groove, the cross section of draw-in groove is the annular of partial cut-out, the shape of the total shaft end of its shape and roller and gear adapts, the total shaft end of roller and gear is fixed in the draw-in groove, tooth bar is fixed on the compression adjustment frame, and wheel and rack is meshed.
The roll extrusion deceleration loading device that is used for the cartilage external structure of the present utility model by stepper-motor, leading screw control roller rolling speed, form the roller pure rolling by gear and tooth bar, and roller can roll across culture with uniform acceleration.In addition, the relatively sliding by wedge block can guarantee the parallel lifting of working face, thereby can produce uniform, adjustable draught to culture.The kinestate at this device simulation knee joint place because roll extrusion loads the strained condition that more approaches actual cartilage, therefore can form the research model of artificial cartilage mechanics influence under the roll extrusion loading environment, help the exploration of the power biology aspect of cartilage.
Description of drawings
Figure 1 shows that the perspective view of roll extrusion deceleration loading device of the present utility model;
Figure 2 shows that the side schematic view of roll extrusion deceleration loading device of the present utility model, wherein do not show culturing room and stepper-motor;
Figure 3 shows that the perspective view of lifting device in the roll extrusion deceleration loading device of the present utility model;
Figure 4 shows that the perspective view of leading screw moving system in the roll extrusion deceleration loading device of the present utility model.
Among the figure:
1. stepper-motor; 2. motor shaft; 3. screw mandrel;
4. web member; 5. connecting rod; 6. roller;
7. gear; 8. tooth bar; 9. compression adjustment frame;
10. platform; 11. adjusting screw(rod); 12. the 1st wedge block;
13. the 2nd wedge block; 14. pressing plate; 15. culturing room;
16. underframe; 17. return spring; 18. culture
19. draw-in groove
Embodiment
The utility model is a kind of deceleration loading device of joint cartilage external structure.The loading procedure of roll extrusion can be provided culture, and its one-piece construction as shown in Figure 1.Referring to Fig. 1, roll extrusion deceleration loading device of the present utility model divides from function and comprises two great institutions, that is: rolling controlling organization and compression adjustment mechanism.
At first the rolling controlling organization is described below.As shown in Figure 1 and Figure 2, described rolling controlling organization comprises: stepper-motor 1, motor shaft 2, leading screw 3, web member 4, connecting rod 5, roller 6, gear 7 and tooth bar 8.Stepper-motor 1 is connected with leading screw 3 by motor shaft 2, and the other end of leading screw 3 is connected with connecting rod 5 by web member 4.The end of connecting rod 5 is provided with draw-in groove 19 (see figure 4)s, the cross section of draw-in groove 19 is the annular of partial cut-out, its shape and roller 6 adapt with the shape of the total shaft end of gear 7, roller 6 is fixed in the draw-in groove with the total shaft end of gear 7, tooth bar 8 is fixed on the compression adjustment frame 9, gear 7 and tooth bar 8 engagements.
The rolling control process of above-mentioned rolling controlling organization is as follows: motor shaft 2 rotations of stepper-motor 1, cause that leading screw 3 moves back and forth along motor shaft 2 and axis direction, leading screw 3 drivening rods 5, roller 6 and gear 7 move together, gear 7 and tooth bar 8 engagements, and gear 7, roller 6 are done pure rolling.Culture 18 is arranged on below the roller 6, so roller 6 rollback that comes up on the surface of culture 18 is moving.The rolling speed of roller 6 is different and different with the velocity of rotation of stepper-motor 1.
Above-mentioned rolling control drives leading screw by stepper-motor and causes that roller rolls back and forth and realize.The motion that drives roller with leading screw can make the central shaft of roller obtain constant speed, and make roller on culture at the uniform velocity making pure rolling, thereby guaranteed that culture is stressed evenly.
Compression adjustment mechanism to the roll extrusion deceleration loading device describes below.
As Fig. 1～shown in Figure 4, described compression adjustment mechanism comprises: compression adjustment frame 9, platform 10, adjusting screw(rod) the 11, the 1st wedge block the 12, the 2nd wedge block 13, pressing plate 14, culturing room 15, underframe 16 and return spring 17.Compression adjustment frame 9 has the cross section that is similar to U-shaped, and two side arm passes two rectangular openings that form on the platform 10 respectively, extends upwardly to above the platform 10 below platform 10.The inboard of two side arm upper ends of compression adjustment frame 9 is set with a pressing plate 14 respectively.Pressing plate 14 guarantees that the draw-in groove of connecting rod 5 blocks the axle of roller 6.Roller 6 remains unchanged with compression adjustment frame 9 position in vertical direction, be that roller 6 raises simultaneously along with two relatively moving of wedge block with compression adjustment frame 9 or reduces, the distance of roller 6 and platform 10 is exactly the thickness that the thickness of culture 18 adds culturing room's base plate.But the lower surface of compression adjustment frame 9 contacts with the upper surface of the 1st wedge block 12 and relatively sliding, and the lower surface of the upper surface of the 1st wedge block 12, compression adjustment frame 9 is all parallel with platform 10.But the inclined-plane that is positioned at the bottom of the 1st wedge block 12 overlaps with the superposed inclined-plane of the 2nd wedge block 13 and relatively sliding.The 1st wedge block 12 and the 2nd wedge block 13 are contained in underframe 16 inside, and a pair of adjusting screw(rod) 11 is fixed on underframe 16 both sides.Two adjusting screw(rod)s 11 are connected with the end of the 1st wedge block 12 with the 2nd wedge block 13 respectively, can promote/spur two wedge blocks, make it produce relative movement.The waist of compression adjustment frame 9, promptly the top of bottom surface is connected with the bottom surface of platform 10 by return spring 17.
The compression adjustment working process of mechanism is: the adjusting screw(rod) 11 inside turns of the 1st wedge block 12, and promote the 1st wedge block 12 and on the inclined-plane of the 2nd wedge block 13, slide, the 1st wedge block 12 whole risings, thus compression adjustment frame 9 is risen.In like manner, also can promote 9 risings of compression adjustment frame by regulating another adjusting screw(rod) 11.Roller 6 remains unchanged the position in vertical direction with compression adjustment frame 9, and roller 6 just increases with the distance of base plate, and the draught of culture 18 just diminishes.When the outside turn of adjusting screw(rod) 11, spur the 1st wedge block 12 on the inclined-plane of the 2nd wedge block 13 to lower slip, the 1st wedge block 12 is whole to descend, return spring 17 promotes compression adjustment framves 9 and descends, thereby reduced the distance of roller 6 with base plate, the draught of culture 18 increases.The different draughts of culture 18 have so just been realized.
For the ease of putting into incubator, the physical dimension of roll extrusion deceleration loading device of the present utility model (removing motor controling part exceptionally) can be done enough for a short time, for example, can be 200mm * 120mm * 120mm.When working, described roll extrusion deceleration loading device puts into existing incubator on the market, at the standard temperature CO of incubator
2Carry out under the concentration conditions.
Claims (2)
1. roll extrusion deceleration loading device that is used for the cartilage external structure, it is characterized in that: comprise the compression adjustment mechanism and the rolling controlling organization that is used for culture is applied roll extrusion load that are used to regulate to the draught of culture, described compression adjustment mechanism comprises: the compression adjustment frame, platform, adjusting screw(rod), the 1st wedge block, the 2nd wedge block, pressing plate, culturing room, underframe and return spring, wherein, the compression adjustment frame has the cross section that is similar to U-shaped, two side arm passes two rectangular openings that form on the platform respectively, below platform, extend upwardly to above the platform, the inboard, upper end of two side arms of compression adjustment frame is set with a pressing plate respectively, in underframe, be provided with the 1st wedge block and the 2nd wedge block, a pair of adjusting screw(rod) is fixed on the both sides of underframe, two adjusting screw(rod)s are connected with an end of the 1st wedge block and the 2nd wedge block respectively, make it produce relative movement, the top of the bottom surface of compression adjustment frame is connected with the bottom surface of platform by return spring, but the lower surface of compression adjustment frame contacts with the upper surface of the 1st wedge block and relatively sliding, the upper surface of the 1st wedge block, the lower surface of compression adjustment frame is all parallel with platform, but the inclined-plane that is positioned at the bottom of the 1st wedge block overlaps with the superposed inclined-plane of the 2nd wedge block and relatively sliding; Described rolling controlling organization comprises stepper-motor, motor shaft, leading screw, web member, connecting rod, roller, gear and tooth bar, described stepper-motor is connected with leading screw by motor shaft, the other end of leading screw is connected with connecting rod by web member, the end of connecting rod is provided with draw-in groove, the cross section of draw-in groove is the annular of partial cut-out, the shape of the total shaft end of its shape and roller and gear adapts, the total shaft end of roller and gear is fixed in the draw-in groove, tooth bar is fixed on the compression adjustment frame, and wheel and rack is meshed.
2. according to each described roll extrusion deceleration loading device in the claim 1～3, it is characterized in that: the physical dimension of described roll extrusion deceleration loading device is 200mm * 120mm * 120mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201439849U CN201354373Y (en) | 2008-12-05 | 2008-12-05 | Rolling load device for constructing cartilage in vitro |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201439849U CN201354373Y (en) | 2008-12-05 | 2008-12-05 | Rolling load device for constructing cartilage in vitro |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201354373Y true CN201354373Y (en) | 2009-12-02 |
Family
ID=41410715
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008201439849U Expired - Fee Related CN201354373Y (en) | 2008-12-05 | 2008-12-05 | Rolling load device for constructing cartilage in vitro |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201354373Y (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102353589A (en) * | 2011-07-11 | 2012-02-15 | 天津理工大学 | Method for using digital images relevant technology in cartilage mechanical property test |
| CN102943042A (en) * | 2012-12-06 | 2013-02-27 | 天津理工大学 | Combined multifunctional bioreactor |
| CN103066027A (en) * | 2011-08-25 | 2013-04-24 | 丰田自动车株式会社 | Power module, method for manufacturing power module, and molding die |
| CN106386383A (en) * | 2016-11-16 | 2017-02-15 | 贵州飞水利荣科技有限公司 | Kiwifruit planting device |
| CN108795756A (en) * | 2018-05-25 | 2018-11-13 | 南京理工大学 | The dynamic of organizational project articular cartilage rolls culture systems |
| CN108871933A (en) * | 2018-06-29 | 2018-11-23 | 西安工业大学 | The chisel teeth Shuan Ban plane strain mechanism of true triaxial pressure chamber |
-
2008
- 2008-12-05 CN CNU2008201439849U patent/CN201354373Y/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102353589A (en) * | 2011-07-11 | 2012-02-15 | 天津理工大学 | Method for using digital images relevant technology in cartilage mechanical property test |
| CN102353589B (en) * | 2011-07-11 | 2013-05-01 | 天津理工大学 | Method for using digital images relevant technology in cartilage mechanical property test |
| CN103066027A (en) * | 2011-08-25 | 2013-04-24 | 丰田自动车株式会社 | Power module, method for manufacturing power module, and molding die |
| US9059145B2 (en) | 2011-08-25 | 2015-06-16 | Toyota Jidosha Kabushiki Kaisha | Power module, method for manufacturing power module, and molding die |
| CN103066027B (en) * | 2011-08-25 | 2016-08-03 | 丰田自动车株式会社 | Power plant module, the method manufacturing power plant module and mould |
| CN102943042A (en) * | 2012-12-06 | 2013-02-27 | 天津理工大学 | Combined multifunctional bioreactor |
| CN102943042B (en) * | 2012-12-06 | 2014-08-06 | 天津理工大学 | Combined multifunctional bioreactor |
| CN106386383A (en) * | 2016-11-16 | 2017-02-15 | 贵州飞水利荣科技有限公司 | Kiwifruit planting device |
| CN108795756A (en) * | 2018-05-25 | 2018-11-13 | 南京理工大学 | The dynamic of organizational project articular cartilage rolls culture systems |
| CN108795756B (en) * | 2018-05-25 | 2023-01-24 | 南京理工大学 | Dynamic rolling culture system for tissue engineering articular cartilage |
| CN108871933A (en) * | 2018-06-29 | 2018-11-23 | 西安工业大学 | The chisel teeth Shuan Ban plane strain mechanism of true triaxial pressure chamber |
| CN108871933B (en) * | 2018-06-29 | 2024-01-26 | 西安工业大学 | Wedge-shaped tooth double-plate plane strain mechanism of true triaxial pressure chamber |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN201354373Y (en) | Rolling load device for constructing cartilage in vitro | |
| CN101265466B (en) | Cultivating method used for coronary camber tissue under composite load and bioreactor thereof | |
| US20080026465A1 (en) | Cell Culturing Method Using Biomechanical Stimulation Loading And System Therefor | |
| CN102943042B (en) | Combined multifunctional bioreactor | |
| CN101250501B (en) | Method for cultivating cartilage by simulating knee joint physical environment and biological reactor | |
| Partap et al. | Bioreactors in tissue engineering | |
| Cigan et al. | Nutrient channels and stirring enhanced the composition and stiffness of large cartilage constructs | |
| CN101058791B (en) | Method of culturing culture by roll load and bioreactor | |
| CN101314765B (en) | In vitro cultivation method for artificial cartilage or bone cartilage with different curve and bioreactor thereof | |
| CN101892154A (en) | Pressure-electricity co-stimulation cell culture device | |
| CN201154961Y (en) | Bioreactor applying rolling load and sliding load | |
| CN101899393B (en) | Dynamic load and recirculating perfusion bioreactor | |
| CN201485468U (en) | Loading equipment for cartilage tissue engineering | |
| CN201883096U (en) | Double-frequency compression-radial twisting intervertebral disc tissue engineering bioreactor | |
| CN215103325U (en) | Cell culture is with automatic liquid device that trades | |
| CN201817494U (en) | Dynamic loading and circular perfusion bioreactor | |
| CN103630490B (en) | The multiple physical field integrated test system of in-vitro evaluation is organized towards natural joint | |
| CN103160434B (en) | An automatic cell culture device capable of simulating internal environments of organisms | |
| CN110055175B (en) | Rolling type bioreactor for in vitro cartilage culture | |
| CN201501876U (en) | Loading device for culturing cartilage | |
| CN201485469U (en) | Loading device for double frequency loading of cultures | |
| CN201154962Y (en) | Bioreactor for culturing artificial cartilage | |
| ITBO20110091U1 (en) | BIOREACTOR FOR FUNCTIONAL TEXTILE ENGINEERING | |
| CN101130752B (en) | Culture method of applying load evenly and bioreactor | |
| CN204058481U (en) | A kind of neural axon tractive growing apparatus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20091202 Termination date: 20111205 |