CN102353589A - Method for using digital images relevant technology in cartilage mechanical property test - Google Patents
Method for using digital images relevant technology in cartilage mechanical property test Download PDFInfo
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- CN102353589A CN102353589A CN2011101916990A CN201110191699A CN102353589A CN 102353589 A CN102353589 A CN 102353589A CN 2011101916990 A CN2011101916990 A CN 2011101916990A CN 201110191699 A CN201110191699 A CN 201110191699A CN 102353589 A CN102353589 A CN 102353589A
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Abstract
The invention provides a method for using a digital images relevant technology in a cartilage mechanical property test, which comprises the following steps: 1) taking a cartilage with a subchondral bone as a test sample; 2) fixing the test sample by a clamp, blocking the images observation surface of the cartilage by transparent glass; 3) embedding the dark color nanoparticles on the images observation surface of the cartilage for inhibiting plaques; 4) fixing the left and right clamping positions of the clamp on a micro tensile machine for loading, immersing the clamp which fixes the cartilage in a glassware filled with normal saline during the loading process. The beneficial effects of the present invention comprise that: the test for cartilage mechanical property is carried out by using the advanced digital images relevant technology, the test environment approaches the normal work environment of human body, the influence caused by the typical boundary problem on the test result when using the technology is avoided, and the digital images relevant technology is effectively combined for artificially inhibiting plaques on the observation surface by aiming at the special material cartilage, so that the obtained clear images are used for subsequent data analysis and processing.
Description
Technical field
The present invention relates to cartilage mechanics performance testing technology in the biological tissue, particularly a kind of method in utilization digital picture correlation technique in the cartilage Mechanics Performance Testing.
Background technology
[0002] along with the development of computer technology, modern optical electronic technology and Digital Image Processing level, Digital Image Correlation Method becomes a kind of important method of testing in Experimental Mechanics field day by day.Biological tissue is different from general material, relative other materials, and its uniqueness just is its complex structure, forms various.Biological tissue and cartilage generally are made up of the solid coupling material of stream, and the live body mechanical environment is very complicated, in the process that its mechanical property is tested, require strict.Suitable mechanical test is related to the important fields such as development to the understanding of organizing various aspects of performance, in vitro culture, disease treatment and medical industry.And, be able to satisfy test to a great extent to biological tissue in view of the good measurement advantage of digital picture correlation technique.It is simple that Digital Image Correlation Method has the light path of measurement, and lower to the requirement of measurement environment, measured speed is fast, precision is high, and is easy to operate, noncontact, the characteristics of whole audience property.Therefore, increasing in recent years domestic and international researcher uses this method that the research object of oneself is tested, and has obtained considerable achievement.
Articular cartilage is to surround the terminal connective tissue of some bone surface.Adult's articular cartilage look it is relative equilibrium usually, and in fact articular cartilage is to be made up of material with complex, is heterogeneity and anisotropic.Articular cartilage heterogeneity and anisotropy obviously not only are its structure and formation, and are its bio-mechanical property.Cartilage all shows the related variation of the extremely strong degree of depth when compressing with tension.The character of understanding the cartilage biomechanics is very important with behavior to health and the illness that the understanding joint is connected.
The mechanical property of articular cartilage experiment at present main stretching or compression test from the cartilage test specimen that exsomatizes; Because articular cartilage solid, liquid two-phase complex structure; Test specimen obtain, process the reliability that influences cartilage mechanical property result; In relevant bibliographical information, the data that experiment draws are dispersed strong.This is because test specimen has broken away from original indivisible complex environment.Also have and adopt the cartilage that has subchondral bone to experimentize, mainly carry out compression experiment, but these experiments only are the experiments that has reflected cartilage macroscopic view constitutive relation
,The meticulous structure of articular cartilage can be divided into three layers according to collagenous fibres mode arranged evenly, and shallow top layer is positioned at the cartilage top layer, and forms slidingsurface, and collagenous fibres are parallel with articular surface, and cartilage cell's major axis is to parallel with articular surface; The fiber and the surface in middle layer are interspersed at an angle; The collagenous fibres diameter of deep layer is thicker.The mechanical property of zones of different does not provide in these experiments, and the characteristic of macro-mechanical property does not have different layers field forces scholarship and moral conduct for making an explanation yet.Therefore adopt the mechanical property in digital correlation technical research articular cartilage macro-mechanical property and zone, obtain the mechanical property rule of cartilage both macro and micro.
When adopting digital correlation technical research articular cartilage mechanical property, the articular cartilage transversal section is the image acquisition face.Ubiquitous problem is: articular cartilage quality white, images acquired face monumented point, and need suitable dark particles to form blob features; The articular cartilage size is less, and loading head acts on that the contact position causes boundary effect on the cartilage, causes the error of cartilage Mechanics Performance Testing; Cartilage is the solid coupling material of stream in addition, during the cartilage test in a organized way flow go out, make the image blurring of collection, the enforcement of digital correlation is adopted in influence, these disadvantages affect the test of cartilage mechanical property.Adopt the digital picture correlation technique to carry out in the research of macroscopic view and two kinds of measurement of angle of microcosmic so far both at home and abroad; Although broken through all limitations of conventional test methodologies, however solve the influence of boundary effect to the result, better simulated in vivo environment and the balance that obtains on the clear reliable ramification problem are also not enough.The present invention just is devoted to solve the problem of these aspects, and it is reliable that this test is more improved, and the result is more accurate.
Summary of the invention
The object of the invention is intended to for overcoming the deficiency of prior art, and provide a kind of in the cartilage Mechanics Performance Testing method of utilization digital picture correlation technique, but this method makes measuring process more near the internal environment of human body, test result is more accurate.
Technical scheme of the present invention:
A kind of method of in the cartilage Mechanics Performance Testing, using the digital picture correlation technique, step is following:
1) get have subchondral bone cartilage as test sample book;
2), and the image viewing face of cartilage blocked with clear glass the test sample book clamps;
3) embed dark nano particle system spot on the image viewing surface of cartilage, system spot density is 200-300/mm
2
4) left and right sides retaining part with anchor clamps is fixed on implementation loading on the miniature spring machine, and the anchor clamps that in loading procedure, keep having fixed cartilage are immersed in the glassware that fills physiological saline.
Said dark nano particle is a black NiO nano particle, and particle diameter is 10 nanometers.
The said physiological saline concentration that is weight percentage is 0.9% sodium-chloride water solution.
Said anchor clamps comprise right hold assembly, left hold assembly and clamping plate composition; Right hold assembly is U type structure; The upper plate of right hold assembly is provided with view window and two bolt holes; The lower surface that upper plate is provided with the view window position is provided with the rectangle concave surface; Be provided with the protruding key that the cross section is a rectangle above the lower plate of right hold assembly; The upper plate right side is provided with two and connects screw, and front side board is provided with two and regulates screw; Left side hold assembly is the stepped appearance plate structure; Left side hold assembly is between the upper plate and lower plate of U type structure at right hold assembly; Left side hold assembly lower surface is provided with groove that the cross section is a rectangle and is slidingly matched with the protruding key of right hold assembly; The leading flank of left side hold assembly is provided with semicircle arc convex surface and is used for cartilage is loaded, and the left side of left hold assembly is provided with two and connects screw; Clamping plate are rectangular; Be positioned at right hold assembly upper plate below; Clamping plate are provided with two bolt holes and match with two bolt holes of right hold assembly upper plate; Cartilage places between right hold assembly upper plate and the clamping plate and passes through bolt and clamps, through two decrements of regulating screw-in screw depth adjusting cartilage in the screws at right hold assembly front side board; Clear glass is located in the rectangle concave surface of upper plate lower surface, and the thickness of clear glass is identical with the rectangle concave surface with width, and the extruding through cartilage behind the clear glass insertion rectangle concave surface is fixed; The glassware that fills physiological saline be positioned at anchor clamps below; Anchor clamps two through right hold assembly upper plate right side connect screw and left hold assembly left side two and are connected screw and are connected with the miniature spring machine with screw.
Technical Analysis of the present invention:
In test, adopt above-mentioned optimization method, when cartilage is loaded, it is observed the surface block, avoiding in the traditional mechanics test process boundary effect, and do not influence test process is carried out the clear observation of the whole audience the influence of accuracy as a result with clear glass; Nano particle is embedded it observe surface system spot, make it in the stand under load process, deform in same pace take place, be convenient to mark, and then obtain the visual and accurate result, for follow-up data analysis and processing are laid a solid foundation with cartilage self; In loading procedure, keep cartilage to be immersed in the physiological saline, tissue fluid gets in the salt solution in the cartilage can not influence images acquired, and in physiological saline, can make its stand under load environment more approach the human internal environment.
The invention has the beneficial effects as follows: the digital picture correlation technique of uses advanced is tested the mechanical property of cartilage; Make test environment near normal operation circumstances in the human body; Typical boundary problem is to the influence of test result when having avoided using this technology; And, be convenient to follow-up data analysis and processing thereby obtain distinct image for combining the digital picture correlation technique to observe surface artificial system spot at it effectively to this special material of cartilage.
Description of drawings
The image that Fig. 1 gathers when directly cartilage being loaded.
Fig. 2 is for blocking the image that the observation surface avoids boundary problem influence back to gather with glass.
Fig. 3 is for observing the image of gathering behind the system spot of surface.
Fig. 4 is an anchor clamps three-dimensional structure synoptic diagram.
Fig. 5 is the right hold assembly three-dimensional structure synoptic diagram of anchor clamps.
Fig. 6 is the left hold assembly three-dimensional structure synoptic diagram of anchor clamps.
Fig. 7 is the clamp working-station vertical view.
Among the figure: 1. right hold assembly 2. left hold assembly 3. clamping plate 4. view windows 5. bolt holes 6. rectangle concave surfaces 7. protruding key 8-I, 8-II. connect screw 9. and regulate screw 10. grooves
11. arc convex surface 12. cartilages 13. clear glasses 14. glasswares.
Embodiment
Embodiment:
A kind of method of in the cartilage Mechanics Performance Testing, using the digital picture correlation technique; The used anchor clamps of test sample book among this embodiment; As shown in Figure 4; Comprise right hold assembly 1; Left side hold assembly 2 is formed with clamping plate 3; Right hold assembly 1; As shown in Figure 5; Be U type structure; The upper plate of right hold assembly 1 is provided with view window 4 and two bolt holes 5; The lower surface that upper plate is provided with the view window position is provided with rectangle concave surface 6; Be provided with the protruding key 7 that the cross section is a rectangle above the lower plate of right hold assembly 1, the upper plate right side is provided with two and connects screw 8-I, and front side board is provided with two and regulates screw 9; Left side hold assembly 2; As shown in Figure 6; Be the stepped appearance plate structure; Left side hold assembly 2 is between the upper plate and lower plate of U type structure at right hold assembly 1; Left side hold assembly 2 lower surfaces are provided with groove 10 that the cross section is a rectangle and are slidingly matched with the protruding key 7 of right hold assembly 1; The leading flank of left side hold assembly 2 is provided with semicircle arc convex surface 11 and is used for cartilage 12 is loaded, and the left side of left hold assembly 2 is provided with two and connects screw 8-II; Clamping plate 3 are rectangular; Be positioned at right hold assembly 1 upper plate below; Clamping plate 3 are provided with two bolt holes and match with two bolt holes 5 of right hold assembly 1 upper plate; Cartilage 12 places between right hold assembly 1 upper plate and the clamping plate and passes through bolt and clamps, through two decrements of regulating screw-in screw depth adjusting cartilage 12 in the screws 9 at right hold assembly 1 front side board; Clear glass 13 is located in the rectangle concave surface 6 of upper plate lower surface, and the thickness of clear glass 13 is identical with rectangle concave surface 6 with width, and clear glass 13 insertion rectangle concave surfaces 6 backs are fixed through the extruding of cartilage 12; The glassware 14 that fills physiological saline be positioned at anchor clamps below, as shown in Figure 7; Anchor clamps two through right hold assembly 1 upper plate right side connect screw 8-I and left hold assembly 2 left sides two and are connected screw 8-II and are connected with the miniature spring machine with screw.
Utilize above-mentioned anchor clamps and adopt this method that pig coaster position articular cartilage is carried out Mechanics Performance Testing, step is following:
1) get fresh pig coaster position articular cartilage, it accomplished the test sample book that long 6cm, wide 3cm, thickness are about 2cm;
2), fix clear glass through the cartilage extruding simultaneously and observe with being convenient to avoid boundary problem the aforementioned clamps of test sample book;
3) embedding particle diameter on the image viewing surface of cartilage is the black NiO nano particle system spot of 10 nanometers, and system spot density is 200-300/mm
2
4) anchor clamps are fixed through the connection screw and the miniature spring machine at left and right retaining part two ends; Semicircle arc convex surface through left hold assembly under the pulling force of miniature spring machine loads cartilage; The anchor clamps that in loading procedure, keep having fixed cartilage are immersed in and fill in the glassware that weight percent concentration is 0.9% physiological saline; Open Control Software and Cai Tu program; Slide to cartilage and load the limit images acquired in the limit; At last the image and the force curve data that collect are preserved, in order to subsequent treatment and analysis.
Experimental result proves: when directly cartilage being loaded, as shown in Figure 1, by image acquisition to the data related coefficient about about 0.9, but because the reliable experiment result degree of this situation is under suspicion,, still need improve even related coefficient is higher; Avoided after the influence of boundary problem to the result; As shown in Figure 2; Because cartilage is squeezed; Inner tissue fluid effect of flood visual effect; Make the image definition that collects not enough; And then cause related coefficient to descend, and related coefficient be in the digital picture correlation technique to the far-reaching factor of result degree of accuracy, therefore such improvement is still very big defective; Adopt the method for testing of said invention, as shown in Figure 3, the sharpness of image is guaranteed, and has reached the standard of said claim, and the related coefficient of view data with directly the loading close, this makes test result more accurate, experimental result is more convincing.
Claims (4)
1. the method for a utilization digital picture correlation technique in the cartilage Mechanics Performance Testing is characterized in that step is following:
1) get have subchondral bone cartilage as test sample book;
2), and the image viewing face of cartilage blocked with clear glass the test sample book clamps;
3) embed dark nano particle system spot on the image viewing surface of cartilage, system spot density is 200-300/mm
2
4) left and right sides retaining part with anchor clamps is fixed on implementation loading on the miniature spring machine, and the anchor clamps that in loading procedure, keep having fixed cartilage are immersed in the glassware that fills physiological saline.
2. according to the said method of in the cartilage Mechanics Performance Testing, using the digital picture correlation technique of claim 1, it is characterized in that: said dark nano particle is a black NiO nano particle, and particle diameter is 10 nanometers.
According to claim 1 said in the cartilage Mechanics Performance Testing method of utilization digital picture correlation technique, it is characterized in that: the said physiological saline concentration that is weight percentage is 0.9% sodium-chloride water solution.
4. according to the said method of in the cartilage Mechanics Performance Testing, using the digital picture correlation technique of claim 1; It is characterized in that: said anchor clamps comprise right hold assembly, left hold assembly and clamping plate composition; Right hold assembly is U type structure; The upper plate of right hold assembly is provided with view window and two bolt holes; The lower surface that upper plate is provided with the view window position is provided with the rectangle concave surface; Be provided with the protruding key that the cross section is a rectangle above the lower plate of right hold assembly; The upper plate right side is provided with two and connects screw, and front side board is provided with two and regulates screw; Left side hold assembly is the stepped appearance plate structure; Left side hold assembly is between the upper plate and lower plate of U type structure at right hold assembly; Left side hold assembly lower surface is provided with groove that the cross section is a rectangle and is slidingly matched with the protruding key of right hold assembly; The leading flank of left side hold assembly is provided with semicircle arc convex surface and is used for cartilage is loaded, and the left side of left hold assembly is provided with two and connects screw; Clamping plate are rectangular; Be positioned at right hold assembly upper plate below; Clamping plate are provided with two bolt holes and match with two bolt holes of right hold assembly upper plate; Cartilage places between right hold assembly upper plate and the clamping plate and passes through bolt and clamps, through two decrements of regulating screw-in screw depth adjusting cartilage in the screws at right hold assembly front side board; Clear glass is located in the rectangle concave surface of upper plate lower surface, and the thickness of clear glass is identical with the rectangle concave surface with width, and the extruding through cartilage behind the clear glass insertion rectangle concave surface is fixed; The glassware that fills physiological saline be positioned at anchor clamps below; Anchor clamps two through right hold assembly upper plate right side connect screw and left hold assembly left side two and are connected screw and are connected with the miniature spring machine with screw.
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Cited By (7)
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| CN103440423A (en) * | 2013-09-04 | 2013-12-11 | 天津理工大学 | Model for measuring mechanical properties of articular cartilage under action of physiological loads |
| CN104799816A (en) * | 2015-04-17 | 2015-07-29 | 上海交通大学 | Evaluation device for implant damage and fretting damage of neural electrode |
| CN110006790A (en) * | 2019-04-09 | 2019-07-12 | 中国人民解放军总医院 | Cartilage permeability measurement apparatus and measurement method |
| CN110132723A (en) * | 2019-04-23 | 2019-08-16 | 江苏科技大学 | Organization mechanics performance testing device and test method under a kind of imitative biological living environment |
| CN110542634A (en) * | 2018-12-15 | 2019-12-06 | 嘉思特华剑医疗器材(天津)有限公司 | Method for measuring through hole rate of 3D-printed titanium alloy bone trabecula test piece |
| CN112432852A (en) * | 2020-11-19 | 2021-03-02 | 中国医学科学院生物医学工程研究所 | Method for testing mechanical properties of 3D printing tissue engineering auricular cartilage and support |
| CN112683667A (en) * | 2020-12-02 | 2021-04-20 | 北京市理化分析测试中心 | System and method for testing tensile property of soft hydrophilic material in physiological saline |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103440423A (en) * | 2013-09-04 | 2013-12-11 | 天津理工大学 | Model for measuring mechanical properties of articular cartilage under action of physiological loads |
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| CN110542634B (en) * | 2018-12-15 | 2022-05-03 | 嘉思特华剑医疗器材(天津)有限公司 | Method for measuring through hole rate of 3D-printed titanium alloy bone trabecula test piece |
| CN110006790A (en) * | 2019-04-09 | 2019-07-12 | 中国人民解放军总医院 | Cartilage permeability measurement apparatus and measurement method |
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| CN110132723A (en) * | 2019-04-23 | 2019-08-16 | 江苏科技大学 | Organization mechanics performance testing device and test method under a kind of imitative biological living environment |
| CN110132723B (en) * | 2019-04-23 | 2021-07-27 | 江苏科技大学 | Device and method for testing tissue mechanical properties in bionic living body environment |
| CN112432852A (en) * | 2020-11-19 | 2021-03-02 | 中国医学科学院生物医学工程研究所 | Method for testing mechanical properties of 3D printing tissue engineering auricular cartilage and support |
| CN112432852B (en) * | 2020-11-19 | 2022-11-22 | 中国医学科学院生物医学工程研究所 | Method for testing mechanical properties of 3D printing tissue engineering auricular cartilage and support |
| CN112683667A (en) * | 2020-12-02 | 2021-04-20 | 北京市理化分析测试中心 | System and method for testing tensile property of soft hydrophilic material in physiological saline |
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