CN102353589B - Method for using digital images relevant technology in cartilage mechanical property test - Google Patents
Method for using digital images relevant technology in cartilage mechanical property test Download PDFInfo
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- CN102353589B CN102353589B CN 201110191699 CN201110191699A CN102353589B CN 102353589 B CN102353589 B CN 102353589B CN 201110191699 CN201110191699 CN 201110191699 CN 201110191699 A CN201110191699 A CN 201110191699A CN 102353589 B CN102353589 B CN 102353589B
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Abstract
The invention provides a method for using a digital images relevant technology in a cartilage mechanical property test, which comprises the following steps: 1) taking a cartilage with a subchondral bone as a test sample; 2) fixing the test sample by a clamp, blocking the images observation surface of the cartilage by transparent glass; 3) embedding the dark color nanoparticles on the images observation surface of the cartilage for inhibiting plaques; 4) fixing the left and right clamping positions of the clamp on a micro tensile machine for loading, immersing the clamp which fixes the cartilage in a glassware filled with normal saline during the loading process. The beneficial effects of the present invention comprise that: the test for cartilage mechanical property is carried out by using the advanced digital images relevant technology, the test environment approaches the normal work environment of human body, the influence caused by the typical boundary problem on the test result when using the technology is avoided, and the digital images relevant technology is effectively combined for artificially inhibiting plaques on the observation surface by aiming at the special material cartilage, so that the obtained clear images are used for subsequent data analysis and processing.
Description
Technical field
The present invention relates to cartilage mechanics performance testing technology in the biological tissue, particularly a kind of method in the cartilage Mechanics Performance Testing, using the digital picture correlation technique.
Background technology
[0002] along with the development of computer technology, modern optical electronic technology and Digital Image Processing level, Digital Image Correlation Method becomes a kind of important method of testing in Experimental Mechanics field day by day.Biological tissue is different from general material, relative other materials, and its uniqueness just is its complex structure, forms various.Biological tissue and cartilage generally are comprised of the solid coupling material of stream, and the live body mechanical environment is very complicated, requires stricter in the process that its mechanical property is tested.Suitable mechanical test is related to the important fields such as development to the understanding of organizing various aspects of performance, in vitro culture, disease treatment and medical industry.And in view of the good measurement advantage of digital picture correlation technique, be able to satisfy to a great extent the test to biological tissue.Digital Image Correlation Method has that optical path is simple, and lower to the requirement of measurement environment, the speed of measurement is fast, precision is high, and is easy to operate, noncontact, the characteristics of the whole audience.Therefore, increasing researcher both at home and abroad used this method that the research object of oneself is tested in recent years, and had obtained considerable achievement.
Articular cartilage is to surround the connective tissue of some bone surface end.Become human articular cartilage, usually look it is relative equilibrium, and in fact articular cartilage is to be made of material with complex, is heterogeneity and anisotropic.Articular cartilage heterogeneity and anisotropy obviously not only are its structure and formation, and are its bio-mechanical property.Cartilage all shows the related variation of the extremely strong degree of depth when compressing with tension.The character of understanding Cartilage biomechanics is connected health and the illness of understanding the joint connection with behavior be very important.
The at present mechanical property of articular cartilage experiment main stretching or compression test from the cartilage test specimen that exsomatizes, because articular cartilage solid, liquid two-phase complex structure, test specimen obtain, process the reliability that affects cartilage mechanical property result, in relevant bibliographical information, the data that experiment draws are dispersed strong.This is because test specimen has broken away from original indivisible complex environment.Also have to adopt and test with the cartilage of subchondral bone, mainly carry out compression experiment, but these experiments only are the experiments that has reflected the cartilage macroscopic const itutive relation
,The meticulous structure of articular cartilage can be divided into three layers according to collagenous fibres mode arranged evenly, and shallow top layer is positioned at the cartilage top layer, and forms slidingsurface, and collagenous fibres are parallel with articular surface, and cartilage cell's major axis is to parallel with articular surface; Fiber and the surface in middle layer are interspersed at an angle; The collagenous fibres diameter of deep layer is thicker.The mechanical property of zones of different does not provide in these experiments, and the feature of macro-mechanical property does not have different layers district mechanical behavior to make an explanation yet.Therefore adopt the mechanical property in digital correlation technical research articular cartilage macro-mechanical property and zone, obtain the mechanical property rule of cartilage both macro and micro.
When adopting digital correlation technical research articular cartilage mechanical property, the articular cartilage transversal section is the image acquisition face.Ubiquitous problem is: articular cartilage quality white, gather the image surface monumented point, and need suitable dark particles to form blob features; The articular cartilage size is less, and loading head acts on that the contact position causes boundary effect on the cartilage, causes the error of cartilage Mechanics Performance Testing; Cartilage is the solid coupling material of stream in addition, during the cartilage test in a organized way liquid flow out, make the image blurring of collection, the enforcement of digital correlation is adopted in impact, these disadvantages affect the test of cartilage mechanical property.Adopt so far the digital picture correlation technique to carry out in the research of macroscopic view and two kinds of measurement of angle of microcosmic both at home and abroad, although broken through all limitations of conventional test methodologies, however solve the impact of boundary effect on the result, better simulated in vivo environment and the balance that obtains on the clear reliable ramification problem are also not enough.The present invention just is devoted to solve the problem of these aspects, makes this test more perfect reliable, and the result is more accurate.
Summary of the invention
Purpose of the present invention is intended to for overcoming the deficiencies in the prior art, and a kind of method of using the digital picture correlation technique in the cartilage Mechanics Performance Testing is provided, but the method makes measuring process more near the internal environment of human body, and test result is more accurate.
Technical scheme of the present invention:
A kind of method of in the cartilage Mechanics Performance Testing, using the digital picture correlation technique, step is as follows:
1) gets cartilage with subchondral bone as test sample book;
2) test sample book is fixed with anchor clamps, and the image viewing face of cartilage is blocked with clear glass;
3) embed dark nano particle spot processed on the image viewing surface of cartilage, spot density processed is 200-300/mm
2
4) left and right sides retaining part with anchor clamps is fixed on implementation loading on the miniature spring machine, and the anchor clamps that keep having fixed cartilage in loading procedure are immersed in the glassware that fills physiological saline.
Described dark nano particle is black NiO nano particle, and particle diameter is 10 nanometers.
The described physiological saline concentration that is weight percentage is 0.9% sodium-chloride water solution.
Described anchor clamps comprise that right hold assembly, left hold assembly and clamping plate form, right hold assembly is U-shaped structure, the upper plate of right hold assembly is provided with view window and two bolt holes, the lower surface that upper plate is provided with the view window position is provided with the rectangle concave surface, being provided with the cross section above the lower plate of right hold assembly is the protruding key of rectangle, the upper plate right side is provided with two connecting screw holes, and front side board is provided with two and regulates screw; Left hold assembly is the ladder sheet part structure, left hold assembly is between the upper plate and lower plate of U-shaped structure at right hold assembly, left hold assembly lower surface is provided with groove that the cross section is rectangle and is slidingly matched with the protruding key of right hold assembly, the leading flank of left hold assembly is provided with semicircle arc convex surface and is used for cartilage is loaded, and the left side of left hold assembly is provided with two connecting screw holes; Clamping plate are rectangular, be positioned at right hold assembly upper plate below, clamping plate are provided with two bolt holes and match with two bolt holes of right hold assembly upper plate, cartilage places between right hold assembly upper plate and the clamping plate and by bolt and anchor clamps and fixes, by two decrements of regulating screw-in screw depth adjusting cartilage in the screw at right hold assembly front side board; Clear glass is located in the rectangle concave surface of upper plate lower surface, and the thickness of clear glass is identical with the rectangle concave surface with width, and the extruding by cartilage behind the clear glass insertion rectangle concave surface is fixed; The glassware that fills physiological saline be positioned at anchor clamps below; Two connecting screw hole of anchor clamps by right hold assembly upper plate right side be connected two connecting screw holes of hold assembly left side and be connected with the miniature spring machine with screws.
Technical Analysis of the present invention:
In test, adopt above-mentioned optimization method, when cartilage is loaded, it is observed the surface block with clear glass, avoiding in the traditional mechanics test process boundary effect on the as a result impact of accuracy, and do not affect test process is carried out the clear observation of the whole audience; Nano particle is embedded it observe surface spot processed, make it in the stand under load process, with cartilage self deform in same pace occur, be convenient to mark, and then obtain intuitively accurately result, for follow-up data analysis and processing are laid a solid foundation; Keep cartilage to be immersed in the physiological saline in loading procedure, tissue fluid enters in the salt solution in the cartilage can not affect the collection image, and can make its stand under load environment closer to the human internal environment in physiological saline.
The invention has the beneficial effects as follows: the digital picture correlation technique of uses advanced is tested the mechanical property of cartilage, make test environment near normal operation circumstances in the human body, typical boundary problem is on the impact of test result when having avoided using this technology, and for effectively observing surface artificial processed spot for this special material of cartilage at it in conjunction with the digital picture correlation technique, be convenient to follow-up data analysis and processing thereby obtain clearly image.
Description of drawings
The image that Fig. 1 gathers when directly cartilage being loaded.
Fig. 2 avoids the rear image that gathers of boundary problem impact for block the observation surface with glass.
Fig. 3 is for observing the image that gathers behind the spot processed of surface.
Fig. 4 is anchor clamps three-dimensional structure schematic diagram.
Fig. 5 is the right hold assembly three-dimensional structure schematic diagram of anchor clamps.
Fig. 6 is the left hold assembly three-dimensional structure schematic diagram of anchor clamps.
Fig. 7 is the clamp working-station vertical view.
Among the figure: 1. right hold assembly 2. left hold assembly 3. clamping plate 4. view windows 5. bolt holes 6. rectangle concave surfaces 7. protruding key 8-I, 8-II. connecting screw hole 9. is regulated screws 10. grooves
11. arc convex surface 12. cartilages 13. clear glasses 14. glasswares.
Embodiment
Embodiment:
A kind of method of in the cartilage Mechanics Performance Testing, using the digital picture correlation technique, the used anchor clamps of test sample book among this embodiment, as shown in Figure 4, comprise right hold assembly 1, left hold assembly 2 and clamping plate 3 form, right hold assembly 1, as shown in Figure 5, be U-shaped structure, the upper plate of right hold assembly 1 is provided with view window 4 and two bolt holes 5, the lower surface that upper plate is provided with the view window position is provided with rectangle concave surface 6, being provided with the cross section above the lower plate of right hold assembly 1 is the protruding key 7 of rectangle, and the upper plate right side is provided with two connecting screw hole 8-I, and front side board is provided with two and regulates screw 9; Left hold assembly 2, as shown in Figure 6, be the ladder sheet part structure, left hold assembly 2 is between the upper plate and lower plate of U-shaped structure at right hold assembly 1, left hold assembly 2 lower surfaces are provided with groove 10 that the cross section is rectangle and are slidingly matched with the protruding key 7 of right hold assembly 1, the leading flank of left hold assembly 2 is provided with semicircle arc convex surface 11 and is used for cartilage 12 is loaded, and the left side of left hold assembly 2 is provided with two connecting screw hole 8-II; Clamping plate 3 are rectangular, be positioned at right hold assembly 1 upper plate below, clamping plate 3 are provided with two bolt holes and match with two bolt holes 5 of right hold assembly 1 upper plate, cartilage 12 places between right hold assembly 1 upper plate and the clamping plate and by bolt and anchor clamps and fixes, by two decrements of regulating screw 9 interior screw-in screw depth adjusting cartilages 12 at right hold assembly 1 front side board; Clear glass 13 is located in the rectangle concave surface 6 of upper plate lower surface, and the thickness of clear glass 13 is identical with rectangle concave surface 6 with width, and clear glass 13 inserts the 6 rear extruding by cartilage 12 of rectangle concave surface and is fixed; The glassware 14 that fills physiological saline be positioned at anchor clamps below, as shown in Figure 7; Two the connecting screw hole 8-I of anchor clamps by right hold assembly 1 upper plate right side be connected two connecting screw hole 8-II of hold assembly 2 left sides and be connected with the miniature spring machine with screws.
Utilize above-mentioned anchor clamps and adopt this method that pig coaster position articular cartilage is carried out Mechanics Performance Testing, step is as follows:
1) get fresh pig coaster position articular cartilage, it accomplished the test sample book that long 6cm, wide 3cm, thickness are about 2cm;
2) test sample book is fixed with aforementioned anchor clamps, fix clear glass by the cartilage extruding simultaneously and observe to avoid boundary problem and to be convenient to;
3) embedding particle diameter on the image viewing surface of cartilage is the black NiO nano particle spot processed of 10 nanometers, and spot density processed is 200-300/mm
2
4) anchor clamps are fixed by connecting screw hole and the miniature spring machine at left and right retaining part two ends, semicircle arc convex surface by left hold assembly under the pulling force of miniature spring machine loads cartilage, the anchor clamps that keep having fixed cartilage in loading procedure are immersed in and fill in the glassware that weight percent concentration is 0.9% physiological saline, open control software and Cai Tu program, slide to cartilage and load limit collection image in the limit, at last the image and the force curve data that collect are preserved, in order to subsequent treatment and analysis.
The results show: when directly cartilage being loaded, as shown in Figure 1, by image acquisition to the data related coefficient about about 0.9, but because the reliable experiment result degree of this situation is under suspicion, even related coefficient is higher, still need improve; Avoided after the impact of boundary problem on the result, as shown in Figure 2, because cartilage is squeezed, inner tissue fluid effect of flood visual effect, make the image definition that collects inadequate, and then cause related coefficient to descend, and related coefficient be in the digital picture correlation technique to the far-reaching factor of result degree of accuracy, therefore such improvement is still very large defective; Adopt the method for testing of described invention, as shown in Figure 3, the sharpness of image is guaranteed, and has reached the standard of described claim, and the related coefficient of view data with directly the loading close, this is so that test result is more accurate, experimental result is more convincing.
Claims (3)
1. method of in the cartilage Mechanics Performance Testing, using the digital picture correlation technique, step is as follows:
1) gets cartilage with subchondral bone as test sample book;
2) test sample book is fixed with anchor clamps, and the image viewing face of cartilage is blocked with clear glass;
3) embed dark nano particle spot processed on the image viewing surface of cartilage, spot density processed is 200-300/mm
2
4) left and right sides retaining part with anchor clamps is fixed on implementation loading on the miniature spring machine, and the anchor clamps that keep having fixed cartilage in loading procedure are immersed in the glassware that fills physiological saline;
It is characterized in that: described anchor clamps comprise that right hold assembly, left hold assembly and clamping plate form, right hold assembly is U-shaped structure, the upper plate of right hold assembly is provided with view window and two bolt holes, the lower surface that upper plate is provided with the view window position is provided with the rectangle concave surface, being provided with the cross section above the lower plate of right hold assembly is the protruding key of rectangle, the upper plate right side is provided with two connecting screw holes, and front side board is provided with two and regulates screw; Left hold assembly is the ladder sheet part structure, left hold assembly is between the upper plate and lower plate of U-shaped structure at right hold assembly, left hold assembly lower surface is provided with groove that the cross section is rectangle and is slidingly matched with the protruding key of right hold assembly, the leading flank of left hold assembly is provided with semicircle arc convex surface and is used for cartilage is loaded, and the left side of left hold assembly is provided with two connecting screw holes; Clamping plate are rectangular, be positioned at right hold assembly upper plate below, clamping plate are provided with two bolt holes and match with two bolt holes of right hold assembly upper plate, cartilage places between right hold assembly upper plate and the clamping plate and by bolt and anchor clamps and fixes, by two decrements of regulating screw-in screw depth adjusting cartilage in the screw at right hold assembly front side board; Clear glass is located in the rectangle concave surface of upper plate lower surface, and the thickness of clear glass is identical with the rectangle concave surface with width, and the extruding by cartilage behind the clear glass insertion rectangle concave surface is fixed; The glassware that fills physiological saline be positioned at anchor clamps below; Two connecting screw hole of anchor clamps by right hold assembly upper plate right side be connected two connecting screw holes of hold assembly left side and be connected with the miniature spring machine with screws.
2. the described method of using the digital picture correlation technique in the cartilage Mechanics Performance Testing according to claim 1, it is characterized in that: described dark nano particle is black NiO nano particle, and particle diameter is 10 nanometers.
3. the described method of using the digital picture correlation technique in the cartilage Mechanics Performance Testing according to claim 1 is characterized in that: the described physiological saline concentration that is weight percentage is 0.9% sodium-chloride water solution.
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| CN103440423B (en) * | 2013-09-04 | 2016-05-11 | 天津理工大学 | A kind of for measuring the model of physiological loads effect hypozygal cartilage mechanical property |
| CN104799816A (en) * | 2015-04-17 | 2015-07-29 | 上海交通大学 | Evaluation device for implant damage and fretting damage of neural electrode |
| CN110542634B (en) * | 2018-12-15 | 2022-05-03 | 嘉思特华剑医疗器材(天津)有限公司 | Method for measuring through hole rate of 3D-printed titanium alloy bone trabecula test piece |
| CN110006790B (en) * | 2019-04-09 | 2024-05-07 | 中国人民解放军总医院 | Cartilage permeability measuring device and measuring method |
| CN110132723B (en) * | 2019-04-23 | 2021-07-27 | 江苏科技大学 | Device and method for testing tissue mechanical properties in bionic living body environment |
| CN112432852B (en) * | 2020-11-19 | 2022-11-22 | 中国医学科学院生物医学工程研究所 | Method for testing mechanical properties of 3D printing tissue engineering auricular cartilage and support |
| CN112683667A (en) * | 2020-12-02 | 2021-04-20 | 北京市理化分析测试中心 | System and method for testing tensile property of soft hydrophilic material in physiological saline |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2191006A (en) * | 1986-05-22 | 1987-12-02 | Rolls Royce Plc | Testing physical properties of materials e.g. cartilage |
| CN101158679A (en) * | 2007-11-23 | 2008-04-09 | 清华大学 | Extract and mechanical properties measurement method of bone trabecula and measurement mechanism |
| CN201354373Y (en) * | 2008-12-05 | 2009-12-02 | 天津理工大学 | Rolling load device for constructing cartilage in vitro |
| CN101886995A (en) * | 2010-06-09 | 2010-11-17 | 北京航空航天大学 | Bone biomechanical measurement device |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19929578C2 (en) * | 1999-06-21 | 2001-06-07 | Universitaetsklinikum Charite | Method and device for testing cartilage |
-
2011
- 2011-07-11 CN CN 201110191699 patent/CN102353589B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2191006A (en) * | 1986-05-22 | 1987-12-02 | Rolls Royce Plc | Testing physical properties of materials e.g. cartilage |
| CN101158679A (en) * | 2007-11-23 | 2008-04-09 | 清华大学 | Extract and mechanical properties measurement method of bone trabecula and measurement mechanism |
| CN201354373Y (en) * | 2008-12-05 | 2009-12-02 | 天津理工大学 | Rolling load device for constructing cartilage in vitro |
| CN101886995A (en) * | 2010-06-09 | 2010-11-17 | 北京航空航天大学 | Bone biomechanical measurement device |
Non-Patent Citations (6)
| Title |
|---|
| 侯振德等.利用图像相关法测量骨拉伸弹性模量随试件厚度的变化规律.《中国生物医学工程学报》.2002,第21卷(第6期),507-510. |
| 利用图像相关法测量骨拉伸弹性模量随试件厚度的变化规律;侯振德等;《中国生物医学工程学报》;20021231;第21卷(第6期);507-510 * |
| 单鹏程等.软骨下骨在骨关节炎发病机制中的作用.《中国矫形外科杂志》.2009,第17卷(第23期),1792-1795. |
| 张东升.数字图像相关测量技术及其应用.《医用生物力学》.2009,第24卷(第2期),85-88. |
| 数字图像相关测量技术及其应用;张东升;《医用生物力学》;20090430;第24卷(第2期);85-88 * |
| 软骨下骨在骨关节炎发病机制中的作用;单鹏程等;《中国矫形外科杂志》;20091231;第17卷(第23期);1792-1795 * |
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