CN201203612Y - Kit for rapidly detecting spermatozoa activity - Google Patents
Kit for rapidly detecting spermatozoa activity Download PDFInfo
- Publication number
- CN201203612Y CN201203612Y CNU2008200807260U CN200820080726U CN201203612Y CN 201203612 Y CN201203612 Y CN 201203612Y CN U2008200807260 U CNU2008200807260 U CN U2008200807260U CN 200820080726 U CN200820080726 U CN 200820080726U CN 201203612 Y CN201203612 Y CN 201203612Y
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- sample
- swimming pool
- reagent kit
- test
- fast detecting
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Abstract
The utility model discloses a sperm activity fast detecting reagent kit, which comprises a sample collecting part and a test part. The sample collecting part includes a sample collector and a sample swimming pool, wherein the sample collector is circular small-bowl-shaped, the bottom has a concave cylindrical hole; the sample swimming pool is a cylindrical container with sperm swimming solution, the two ends of the container are sealed by sealing films; and the external diameter of the sample swimming pool is slightly smaller than the internal diameter of the hole on the bottom of the sample collector, such that the sample swimming pool can be inserted into the hole on the bottom of the sample collector. The test part includes a test board, dyeing liquor, washing solution and a sampling burette, wherein the test board has an outer casing and a substrate, the center of the outer casing is provided with a test hole and a contrast hole, an absorbent paper, a filtering film and a standard color code are on the substrate, and the color of the standard color code is the color of a 20 million per milliliter active sperm sample added to the test hole of the test board, dyed and washed.
Description
Technical field
The utility model relates to a kind of in-vitro diagnosis class medicine equipment, specifically is a kind of quick detection kit that is applicable to the mankind spermatozoon activity.
Background technology
The fertility expert report, (no contraceptives) can not be conceived in 1 year for about in the world 15% Mr. and Mrs, and 40% in these cases are because male factor causes, also have male sex expert's report, and the incidence of disease of male sterility is approximately 6-12%.Above-mentioned report, the crowd that male sterility is described is a sizable crowd.
The main method of current diagnosis male sterility is the microscopically diagnosis of seminal fluid, and promptly sperm morphological detects, and this also is the goldstandard of male sterility diagnosis.But there are many shortcomings in this method, such as: because Chinese traditional concept is shyly gone the disease of hospital therapy male sterility aspect, thereby has been incured loss through delay treatment.Even gone to hospital, owing to the condition of getting sperm is limited, be subjected to the restriction of environment, method, time, be difficult for obtaining sample.It is more loaded down with trivial details to go to a hospital to see a doctor, must be through a series of programs such as registering, prescribe.
Summary of the invention
Whether qualified the purpose of this utility model be can operate at home in order to overcome the shortcoming of prior art, to provide a kind of, can detect activated sperm quantity spermatozoon activity fast detecting reagent kit fast, easy to use, low-cost.
Second purpose of the present utility model provides a kind of preparation and using method of spermatozoon activity fast detecting reagent kit.
For achieving the above object, the utility model comprises sample collecting part and test portion, and sample collecting partly comprises sample collecting device and sample swimming pool; The column type aperture that rounded little bowl-shape of sample collecting device, bottom have depression to go down; The sample swimming pool is cylindrical container, in be loaded with sperm swimming liquid, the container two ends are all sealed film and are sealed; The external diameter of sample swimming pool is slightly smaller than the internal diameter of the aperture of sample collecting device bottom, and the sample swimming pool can be inserted in the aperture of sample collecting device bottom; Test portion comprises breadboard, dyeing liquor, washing lotion, sampling dropper; Breadboard comprises shell, substrate, shell and substrate are that rectangular PVC plastics are made, and there are test hole and two apertures of control wells in shell central authorities, and thieving paper, filtering membrane and standard colour code are arranged on the described substrate, thieving paper and filtering membrane are positioned at the substrate top, the test hole below of shell; Thieving paper is positioned at the filtering membrane below; The standard colors mark is in the substrate top, below the control wells; Standard colors target color is that 2,000 ten thousand/milliliter activated sample of sperm add the test hole of breadboard through the color after dyeing, washing;
Advantage of the present utility model: whether the quantity that can detect activated sperm rapidly and accurately is qualified, thereby diagnose out the male sex whether can procreate, save a lot of times than traditional in the past diagnostic method, use simple, purchase gets final product in pharmacy, do not spend hospital admission, make the patient not have shy sense, make the man and wife can know the potential fertility Issue of oneself in advance.
Description of drawings
Below in conjunction with accompanying drawing to further detailed explanation of the utility model.
Fig. 1 is the sectional view of the utility model sample collecting device;
Fig. 2 is the structural representation of the utility model sample swimming pool;
Fig. 3 is the structural representation of the utility model breadboard.
1. cylindrical aperture 2. sperms swimming liquid 3. seal film 4. shells 5. substrates 6. test holes 7. control wells 8. filtering membranes 9. thieving papers 10. standard colour codes among the figure
Embodiment
As seen from the figure, the utility model comprises sample collecting part and test portion, and sample collecting partly comprises sample collecting device and sample swimming pool; The column type aperture 1 that rounded little bowl-shape of sample collecting device, bottom have depression to go down; The sample swimming pool is cylindrical container, in be loaded with sperm swimming liquid 2, the container two ends are all sealed film 3 and are sealed; The external diameter of sample swimming pool is slightly smaller than the internal diameter of the cylindrical aperture 1 of sample collecting device bottom, and the sample swimming pool can be inserted in the cylindrical aperture 1 of sample collecting device bottom; Test portion comprises breadboard, dyeing liquor, washing lotion, sampling dropper.Described sample collecting device is 50～60 millimeters of the diameters made by the PVC plastics, high 25～35 millimeters circular little bowl, and the diameter of the column type aperture 1 that the bottom notch of little bowl is gone down is 10～12 millimeters, and is high 15～20 millimeters.Described sample swimming pool is that the clear PET plastics are made, and external diameter is 9～11 millimeters, and internal diameter is 7～9 millimeters, and height is 60～80 millimeters.Breadboard comprises shell 4, substrate 5, shell 4 and substrate 5 are that rectangular PVC plastics are made, there are test hole 6 and 7 two apertures of control wells in shell central authorities, thieving paper 9, filtering membrane 8 and standard colour code 10 are arranged on the described substrate, thieving paper 9 and filtering membrane 8 are positioned at substrate 5 tops, test hole 6 belows of shell 4; Thieving paper 9 is positioned at filtering membrane 8 belows; The standard colors mark is in substrate 5 tops, control wells 7 belows.The color of standard colour code 10 is that 2,000 ten thousand/milliliter activated sample of sperm add the test hole of breadboard through the color after dyeing, washing.Described dyeing liquor is the 0.1-0.5% methylene blue solution.Described washing lotion is a physiological saline, also can be purified water and the destainer that contains acidic materials such as low-concentration acetic acid.Described sperm swimming liquid 2 is acrylamide chlorination sodium solution.
Use the method for spermatozoon activity fast detecting reagent kit: comprising following steps:
Collect semen sample in sample collecting device;
Treat that semen sample liquefaction back flows in the aperture of sample collecting device bottom, the general time is 30 to assign to 60 minutes;
Open the film that seals of sample swimming pool, because of swimming liquid is gel, has surface tension, so open and seal that swimming liquid can not flow out at once behind the film from the sample swimming pool, the sample swimming pool is inserted the aperture of sample collecting device bottom, activated sperm can upwards move about along swimming liquid, and accumulates in the upper strata of swimming liquid;
Add the swimming liquid on a sample swimming pool upper strata with the sampling dropper, join in the test hole of breadboard, allow test hole inner filtration film fully invade wet 1 minute;
Add dyeing liquor, add two dyeing liquors, allow test hole inner filtration film fully invade wet 1 minute to test hole;
Add washing lotion, add two washing lotions, allow test hole inner filtration film fully invade wet 1 minute to test hole;
The change color in viewing test hole:
The test hole blueness is darker than control wells, shows spermatozoon activity 〉=2,000 ten thousand/milliliter
The test hole blueness is blue more shallow than control wells, shows spermatozoon activity＜2,000 ten thousand/milliliter
To in 3 minutes, read the result, inaccurate above reading the possibility of result after 3 minutes;
Test for the second time: per twice test is at interval more than the week.
Claims (8)
1, a kind of spermatozoon activity fast detecting reagent kit comprises sample collecting part and test portion, and it is characterized in that: sample collecting partly comprises sample collecting device and sample swimming pool; The column type aperture that rounded little bowl-shape of sample collecting device, bottom have depression to go down: the sample swimming pool is cylindrical container, in be loaded with sperm swimming liquid, the container two ends are all sealed film and are sealed; The external diameter of sample swimming pool is slightly smaller than the internal diameter of the aperture of sample collecting device bottom, and the sample swimming pool can be inserted in the aperture of sample collecting device bottom; Test portion comprises breadboard, dyeing liquor, washing lotion, sampling dropper.
2, a kind of spermatozoon activity fast detecting reagent kit according to claim 1, it is characterized in that: described sample collecting device is 50～60 millimeters of the diameters made by the PVC plastics, high 25～35 millimeters circular little bowl, the diameter of the column type aperture that the bottom notch of little bowl is gone down is 10～12 millimeters, and is high 15～20 millimeters.
3, a kind of spermatozoon activity fast detecting reagent kit according to claim 1 is characterized in that: described sample swimming pool is that the clear PET plastics are made, and external diameter is 9～11 millimeters, and internal diameter is 7～9 millimeters, and height is 60～80 millimeters.
4, a kind of spermatozoon activity fast detecting reagent kit according to claim 1, it is characterized in that: breadboard comprises shell, substrate, shell and substrate are that rectangular PVC plastics are made, there are test hole and two apertures of control wells in shell central authorities, thieving paper, filtering membrane and standard colour code are arranged on the described substrate, thieving paper and filtering membrane are positioned at the substrate top, the test hole below of shell; Thieving paper is positioned at the filtering membrane below; The standard colors mark is in the substrate top, below the control wells.
5, a kind of spermatozoon activity fast detecting reagent kit according to claim 4 is characterized in that: standard colors target color is that 2,000 ten thousand/milliliter activated sample of sperm add the test hole of breadboard through the color after dyeing, washing.
6, a kind of spermatozoon activity fast detecting reagent kit according to claim 1 is characterized in that: described dyeing liquor is the 0.1-0.5% methylene blue solution.
7, a kind of spermatozoon activity fast detecting reagent kit according to claim 1 is characterized in that: described washing lotion is a physiological saline, also can be purified water and the destainer that contains acidic materials such as low-concentration acetic acid.
8, a kind of spermatozoon activity fast detecting reagent kit according to claim 1 is characterized in that: described sperm swimming liquid is acrylamide chlorination sodium solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200807260U CN201203612Y (en) | 2008-05-21 | 2008-05-21 | Kit for rapidly detecting spermatozoa activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200807260U CN201203612Y (en) | 2008-05-21 | 2008-05-21 | Kit for rapidly detecting spermatozoa activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201203612Y true CN201203612Y (en) | 2009-03-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008200807260U Expired - Fee Related CN201203612Y (en) | 2008-05-21 | 2008-05-21 | Kit for rapidly detecting spermatozoa activity |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201203612Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101271105B (en) * | 2008-05-21 | 2012-12-19 | 北京望升伟业科技发展有限公司 | Spermatozoon activity fast detecting reagent kit and its preparation and use method |
-
2008
- 2008-05-21 CN CNU2008200807260U patent/CN201203612Y/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101271105B (en) * | 2008-05-21 | 2012-12-19 | 北京望升伟业科技发展有限公司 | Spermatozoon activity fast detecting reagent kit and its preparation and use method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090304 Termination date: 20100521 |