CN202994620U - Semi-quantification sperm density detection kit - Google Patents
Semi-quantification sperm density detection kit Download PDFInfo
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- CN202994620U CN202994620U CN 201220652523 CN201220652523U CN202994620U CN 202994620 U CN202994620 U CN 202994620U CN 201220652523 CN201220652523 CN 201220652523 CN 201220652523 U CN201220652523 U CN 201220652523U CN 202994620 U CN202994620 U CN 202994620U
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Abstract
The utility model discloses a semi-quantification sperm density detection kit which comprises a semen fluid collecting and metering container, cell staining fluid, scrubbing solution, a detecting plate and a dropper. The detecting plate comprises a casing and a substrate, a color atla hole and three detecting holes are formed in the center of the casing, a filtering film and a standard color atla are adhered to the substrate, and the filtering film is located above the substrate and below the detecting holes of the casing. The standard color atla is located above the substrate and below the color atla hole, and a color of the standard color atla is a color of dyeing results of 15000000 per milliliter of sperm density samples. The semi-quantification sperm density detection kit can rapidly and accurately conduct qualitative detection on whether sperm density is normal or not, uses 5000000 per milliliter to serve as a range unit, can rapidly and accurately judge several density stages such as aspermatism, oligospermia, seriou oligospermia and over-high sperm density, and accordingly achieves a purpose of semi-quantification rapid detection.
Description
Technical field
The utility model relates to a kind of in-vitro diagnosis class medicine equipment, particularly relates to a kind of semi-quantitative sperm Density Detection kit.
Background technology
At present, about 15% Mr. and Mrs can not be conceived in 1 year in the world, and 40% in these cases are because male factor causes.Also have Andriatrics Dept. expert report, the incidence of disease of male sterility is approximately 6-12%.The crowd of above-mentioned report explanation male sterility is a sizable crowd.The main method of semen quality inspection at present is to use microscope, be that sperm morphological detects, this is also the standard that present semen quality checks, but there are many shortcomings in this method, for example need to go to check to hospital, even gone to hospital, also because the condition of sampling is limited, be subjected to the restriction of environment, method, time, be difficult for obtaining sample, therefore, the inspection of semen quality usually is subject to the restriction of subjectivity and objectivity condition, thereby has delayed treatment.
Therefore how to obtain a kind ofly can operate at home, quick, easy to use and low-cost sperm concentration self check instrument, just seem particularly important, existing self check instrument is a kind of sperm concentration quick diagnosis reagent kit, but it can only be greater than or less than 2,000 ten thousand/milliliter to sperm concentration and do qualitative detection, and error burst is 5,000,000/milliliter, what is more important, existing method can not be distinguished few essence, serious essence and patients with azoospermia less, and these information are very important to the abnormal patient of semen quality, so it is restricted when reality is used.
The utility model content
The purpose of this utility model is in order to overcome the shortcoming of prior art, to provide a kind of and can quick semi-quantitatively detect a plurality of sperm concentration grades, and easy to use, low-cost sperm concentration detection kit.
The purpose of this utility model can be achieved through the following technical solutions:
A kind of semi-quantitative sperm Density Detection kit, comprise seminal fluid collecting measuring container, cell dyeing liquid, cleansing solution, check-out console and dropper, described check-out console comprises shell and substrate, shell central authorities have a colour atla hole and three to detect the hole, be attached with filtering membrane and standard colour code on substrate, wherein filtering membrane is positioned at above substrate, the below, detection hole of shell; The standard colors mark is in substrate top, below, colour atla hole, and standard colors target color is the color with the coloration result of 1,500 ten thousand/milliliter sperm concentration sample.
Seminal fluid collecting measuring container of the present utility model can be for the volume of the metering scale wide-mouth plastic cup greater than 15ml.
Described filtration rete is preferably and filters diameter less than the glass fibre membrane of 5 μ m, can tackle diameter is 0.1-3 μ m spermatoblast, acellular composition (being mainly γ-seminoprotcin) and dyestuff can filter, fiber is evenly distributed, cell-volume is large, and water absorbing capacity is large, and chemical stability is good, to the cell avirulence, dyestuff do not had suction-operated.
The shell of described check-out console and substrate are preferably rectangle shell and the substrate that the PVC plastics are made.
Compared with prior art, the utlity model has following advantage:
the utility model adopts above-mentioned four pore structures, can not only do qualitative detection to sperm concentration normal (sperm concentration is greater than 1,500 ten thousand/milliliter) rapidly and accurately, and can be to few essence (sperm concentration 5,000,000/milliliter to 1,500 ten thousand/milliliter), serious few essence (sperm concentration is less than 5,000,000/milliliter) and make judgement rapidly and accurately without essence (or accidental sperm) these density ratings, thereby realized the purpose of sxemiquantitative fast detecting, be conducive to the male sex oneself semen quality is had comparatively objective appraisal, different for the sperm concentration grade, take different therapeutic schemes.
Description of drawings
Fig. 1 is the structural representation of the utility model check-out console.
Fig. 2 is the vertical view of the utility model check-out console.
Be labeled as in figure: the 1-shell, the 2-substrate, 3-colour atla hole B, 4-detects hole A, and 5-detects hole C, and 6-detects hole D, 7-filtering membrane, 8-standard colour code.
Embodiment
Below in conjunction with drawings and Examples, the utility model is described in further detail.
As shown in Fig. 1-2, a kind of semi-quantitative sperm Density Detection kit, comprise seminal fluid collecting measuring container, cell dyeing liquid, cleansing solution, check-out console and dropper, described seminal fluid collecting measuring container is the wide-mouth plastic cup with metering scale, described check-out console comprises shell 1 and substrate 2, shell 1 and substrate 2 are rectangle, are made by the PVC plastics.Shell 1 central authorities have a colour atla hole B3 and three to detect the hole, are respectively and detect hole A4, detect hole C5, detect hole D6, have on substrate with filtering membrane 7 and standard colour code 8, and filtering membrane 7 is for filtering diameter less than the glass fibre membrane of 5 μ m.Filtering membrane 7 is positioned at substrate 2 tops, the detection hole A4 of shell 1, detects hole C5, detects the below of hole D6; Standard colour code 8 is positioned at substrate 2 tops, colour atla hole B3 below, and standard colors target color is the color with the coloration result of 1,500 ten thousand/milliliter sperm concentration sample.
Before experiment, detect hole A, C, D is white, during use, drips the seminal fluid sample after 3 dropping liquids in detecting hole A, then drips 1 of dyeing liquor, reaction is approximately after 1 minute, then drips 3 of cleansing solutions, after solution fully immerses sample pad, observes the color that detects hole A:
(1) approach or be deeper than standard colour code (B hole) when A hole color, representing that sperm concentration greater than 1,500 ten thousand/milliliter, can tentatively be judged as sperm concentration normal.If (need density range more accurately, can drip the seminal fluid sample after 1 dropping liquid in detecting C hole, hole, and drip successively 3 of 1 of dyeing liquor and cleansing solutions, if colour developing approaches or is deeper than the standard colour code, represent that sperm concentration is greater than 4,500 ten thousand/milliliter; If colour developing is shallower than the standard colour code, represent sperm concentration 4,500 ten thousand/milliliter is between 1,500 ten thousand/milliliter.）
(2) when A hole color more shallow than B hole, represent that sperm concentration is less than 1,500 ten thousand/milliliter, can tentatively be judged as few essence or serious few essence, need this moment for further testing, seminal fluid sample in detecting C hole, hole after dropping 6 dropping liquids, and drip successively 1 dyeing liquor and 3 cleansing solutions, observe the color that detects hole C:
If colour developing approaches or is deeper than the standard colour code, represent sperm concentration at 1,500 ten thousand/milliliter between 7,500,000/milliliter, can be judged as aspermia or oligospermia.
If it is more shallow than standard colour code to develop the color, drip 9 seminal fluid samples in detecting D hole, hole, and drip successively 3 of 1 of dyeing liquor and cleansing solutions, if colour developing approaches or be deeper than the standard colour code, represent sperm concentration 7,500,000/milliliter is between 5,000,000/milliliter, can be judged as aspermia or oligospermia; If colour developing is shallower than the standard colour code, represent sperm concentration less than 5,000,000/milliliter, can be judged as serious few essence.
(3) when A hole color be colourless, represent that sperm concentration is less than 5,000,000/milliliter, can tentatively be judged as serious few essence or without essence, need this moment for further testing, drip the seminal fluid sample after 10 dropping liquids in detecting D hole, hole, and drip successively 3 of 1 of dyeing liquor and cleansing solutions, if colour developing is for light blue, represent sperm concentration less than 5,000,000/milliliter, can be judged as serious few essence; If still show colourless, the expression without the essence or accidental sperm.
The above; it is only the better embodiment of the utility model; but protection domain of the present utility model does not limit to therewith; anyly be familiar with those skilled in the art in the technical scope that the utility model discloses; the variation that can expect easily or replacement are within all should being encompassed in protection domain of the present utility model.
Claims (4)
1. semi-quantitative sperm Density Detection kit, comprise seminal fluid collecting measuring container, cell dyeing liquid, cleansing solution, check-out console and dropper, it is characterized in that, described check-out console comprises shell and substrate, shell central authorities have a colour atla hole and three to detect the hole, be attached with filtering membrane and standard colour code on substrate, wherein filtering membrane is positioned at above substrate, the below, detection hole of shell; The standard colors mark is in substrate top, below, colour atla hole, and standard colors target color is the color with the coloration result of 1,500 ten thousand/milliliter sperm concentration sample.
2. semi-quantitative sperm Density Detection kit as claimed in claim 1, is characterized in that, described seminal fluid collecting measuring container is the wide-mouth plastic cup with metering scale.
3. semi-quantitative sperm Density Detection kit as claimed in claim 1, is characterized in that, described filtration rete is for filtering diameter less than the glass fibre membrane of 5 μ m.
4. semi-quantitative sperm Density Detection kit as claimed in claim 1, is characterized in that, the shell of described check-out console and substrate are that rectangular PVC plastics are made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220652523 CN202994620U (en) | 2012-11-30 | 2012-11-30 | Semi-quantification sperm density detection kit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220652523 CN202994620U (en) | 2012-11-30 | 2012-11-30 | Semi-quantification sperm density detection kit |
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| CN202994620U true CN202994620U (en) | 2013-06-12 |
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| CN 201220652523 Expired - Fee Related CN202994620U (en) | 2012-11-30 | 2012-11-30 | Semi-quantification sperm density detection kit |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106932597A (en) * | 2017-03-29 | 2017-07-07 | 山东大学 | Purposes of 1 lysine of generation mass shift of ATP5A1 protein 53s in the few weak smart diagnostic reagent of severe is prepared |
| CN106996979A (en) * | 2017-03-29 | 2017-08-01 | 山东大学 | Purposes of 6 N 113.05347 of AKAP4 protein 18s in the few weak smart diagnostic reagent of severe is prepared |
| CN106996981A (en) * | 2017-03-29 | 2017-08-01 | 山东大学 | Purposes of 6 N 114.04278 of AKAP4 protein 18s in the few weak smart diagnostic reagent of severe is prepared |
| CN106996980A (en) * | 2017-03-29 | 2017-08-01 | 山东大学 | Purposes of the lysine of 733 generation mass shifts of AKAP4 albumen in the few weak smart diagnostic reagent of severe is prepared |
| CN107015005A (en) * | 2017-03-29 | 2017-08-04 | 山东大学 | Purposes of the threonine of 64 generation mass shifts of GAPDHS albumen in the few weak smart diagnostic reagent of severe is prepared |
| CN107036932A (en) * | 2017-03-22 | 2017-08-11 | 李乐 | Enzymatic liquefaction combines the method and device of centrifugation auxiliary half-quantitative detection sperm concentration |
-
2012
- 2012-11-30 CN CN 201220652523 patent/CN202994620U/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107036932A (en) * | 2017-03-22 | 2017-08-11 | 李乐 | Enzymatic liquefaction combines the method and device of centrifugation auxiliary half-quantitative detection sperm concentration |
| CN106932597A (en) * | 2017-03-29 | 2017-07-07 | 山东大学 | Purposes of 1 lysine of generation mass shift of ATP5A1 protein 53s in the few weak smart diagnostic reagent of severe is prepared |
| CN106996979A (en) * | 2017-03-29 | 2017-08-01 | 山东大学 | Purposes of 6 N 113.05347 of AKAP4 protein 18s in the few weak smart diagnostic reagent of severe is prepared |
| CN106996981A (en) * | 2017-03-29 | 2017-08-01 | 山东大学 | Purposes of 6 N 114.04278 of AKAP4 protein 18s in the few weak smart diagnostic reagent of severe is prepared |
| CN106996980A (en) * | 2017-03-29 | 2017-08-01 | 山东大学 | Purposes of the lysine of 733 generation mass shifts of AKAP4 albumen in the few weak smart diagnostic reagent of severe is prepared |
| CN107015005A (en) * | 2017-03-29 | 2017-08-04 | 山东大学 | Purposes of the threonine of 64 generation mass shifts of GAPDHS albumen in the few weak smart diagnostic reagent of severe is prepared |
| CN106932597B (en) * | 2017-03-29 | 2018-07-20 | 山东大学 | Purposes of the lysine of 1 generation mass shift of ATP5A1 protein 53s in preparing the few weak smart diagnostic reagent of severe |
| CN106996981B (en) * | 2017-03-29 | 2018-11-02 | 山东大学 | Purposes of 6 N-114.04278 of AKAP4 protein 18s in preparing the few weak smart diagnostic reagent of severe |
| CN106996979B (en) * | 2017-03-29 | 2018-11-02 | 山东大学 | Purposes of 6 N-113.05347 of AKAP4 protein 18s in preparing the few weak smart diagnostic reagent of severe |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130612 Termination date: 20211130 |
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| CF01 | Termination of patent right due to non-payment of annual fee |