CN106913348B - Pre-diluted vacuum blood collection tube and method for measuring platelet number - Google Patents
Pre-diluted vacuum blood collection tube and method for measuring platelet number Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/153—Devices specially adapted for taking samples of venous or arterial blood, e.g. with syringes
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- A—HUMAN NECESSITIES
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- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150755—Blood sample preparation for further analysis, e.g. by separating blood components or by mixing
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Abstract
The invention discloses a pre-diluted vacuum blood collection tube which comprises a tube body and a tube plug, wherein a tube cap is arranged on the outer side of the tube plug, the sealed tube body is in a vacuum state, scale marks for marking the volume of the tube body are arranged on the outer wall of the tube body, scale values expressed by the scale marks are from 9mL to 10.5mL, the minimum scale value is 0.1mL, and 9mL of sodium chloride injection or compound electrolyte injection is filled in the tube body. The invention also discloses a method for measuring the platelet number of the EDTA-dependent pseudothrombocytopenia patient by using the pre-diluted vacuum blood collection tube. The invention has simple structure and accurate scale mark, uses the liquid without anticoagulant to dilute venous blood, overcomes the self antigen in the structure of the anticoagulant induced activated platelet membrane glycoprotein GPIIb, and achieves the aim of inhibiting platelet aggregation; through the mode of vacuum blood sampling, the operation is simplified, and the pollution of open blood sampling to operators and the environment is prevented.
Description
Technical Field
The invention belongs to the technical field of medical appliances, and particularly relates to a pre-diluted vacuum blood collection tube and a method for measuring the number of platelets of an EDTA-dependent pseudothrombocytopenia patient.
Background
EDTA-dependent pseudothrombocytopenia (EDTA-PTCP) has a prevalence of between 0.09% and 0.21%, and is a phenomenon called pseudothrombocytopenia in which the number of platelets is significantly reduced over time, but the number of platelets is not significantly reduced in the patient itself, and clinical symptoms and signs are detected again by other methods, and the number of platelets is normal, since the number of platelets is detected by a blood cell analyzer after anticoagulation of venous whole blood using EDTA. EDTA-dependent pseudothrombocytopenia cases account for more than 90% of all pseudothrombocytopenia cases, and because EDTA-dependent pseudothrombocytopenia has a low probability of occurrence, no specific population, and blood samples are continuously and repeatedly detected by a full-automatic blood cell analyzer, the number of platelets is very close; the existing full-automatic blood cell analyzer checks blood convention, if platelets gather, abnormal scattered points appear in a scattered point diagram of a DIFF channel of the full-automatic blood cell analyzer, and the full-automatic blood cell analyzer has alarm prompt, but the accuracy of the alarm prompt is poor; in addition, clinical staff, who are unfamiliar with the actual clinical condition of EDTA-dependent pseudothrombocytopenia patients, have resulted in a significant deceptive increase in the number of sham-reduced platelets to both the clinical and clinical doctors. Clinically, the range between critical and normal reference values for platelet count is too large, from 30× 10 9 Up to 125X 10 9 According to the method, the number of platelets is low, the medical risks are high, and the clinician is easy to drive the clinical actions of infusion platelets, suspension operation, bone marrow puncture examination and the like on the patients with the EDTA-dependent pseudo-thrombocytopenia, so that the physical health of the patients is seriously affected, precious blood resources are wasted, the stress of doctor-patient relations is aggravated, and medical disputes and medical accidents are induced. The clinical laboratory is urgently needed in daily work, and the method for rechecking the platelet count of the EDTA-dependent pseudothrombocytopenia patient is simple to operate and accurate and reliable in result. In patients with EDTA-dependent pseudothrombocytopenia, platelet aggregation-induced autoantigens (Ag) are hidden in platelet membrane glycoprotein GPIIb structures, under the condition of in vitro room temperature, anticoagulants (calcium ion chelating agents) can directly induce the platelet membrane glycoprotein GPIIb structures to change, the autoantigens (Ag) hidden in the glycoprotein GPIIb structures are exposed, the exposed platelet autoantigens (Ag) are identified and combined by anti-platelet autoantibodies (Ab) existing in the patients, the change of GPIIb/IIIa receptor structures on platelet membranes is induced, fibrinogen receptors are activated and exposed, fibrinogen existing in blood directly bridges platelets, and the platelets are aggregated in vitro, so that the platelet aggregation is bigger and bigger along with the time extension, and the platelet number is obviously reduced. EDTA-dependent pseudothrombocytopenia is an abnormal phenomenon induced by anticoagulant (calcium ion chelator) and initiated by platelet autoantigen antibody, directly involved in fibrinogen in blood and fibrinogen receptor on platelet membrane, and occurs under the condition of long-term standing at room temperature in vitro, so that more than 95% of EDTA-dependent pseudothrombocytopenia patients appear in hospitalized patients. Referring to the related literature, it is verified that EDTA-dependent pseudothrombocytopenia patients can be corrected, and platelets induced by anticoagulants (calcium ion chelating agents) in vitro The method for gathering and ensuring accurate and reliable platelet number detection mainly comprises the following three steps:
first, use blue cap vacuum blood collection tube containing 200. Mu.L 3.2% sodium citrate anticoagulant, or 3.8mg EDTAK with 200. Mu.L amikacin sulfate injection 2 The method for detecting the number of platelets by using a full-automatic blood cell analyzer whole blood mode includes the steps of collecting venous blood by a purple head cap vacuum blood collection tube of an anticoagulant. The specific operation flow is that a blue head cap vacuum blood collection tube containing 200 mu L of 3.2% sodium citrate anticoagulant or an injection containing 3.8mg EDTAK and 200 mu L of amikacin sulfate injection is used 2 The method comprises the steps of collecting venous blood again by a purple head cap vacuum blood collection tube of an anticoagulant, and carrying out routine blood detection by using a full-automatic blood cell analyzer whole blood mode to obtain the number of platelets. The method for rechecking the number of the platelets by using the vacuum blood collection tube containing 200 mu L of 3.2% of anticoagulated blue head cover containing sodium citrate has the advantages of simple operation, good repeatability of platelet count, closed blood collection process and no contact of staff with blood, and is most used in daily work of clinical laboratory. However, in blood of EDTA-dependent pseudothrombocytopenia patients, in a vacuum blood collection tube of a blue head cap containing 200 μl of 3.2% sodium citrate anticoagulant, there is a phenomenon that platelets are mildly aggregated, and a large platelet aggregation is visible at the tail of the sheet, which seriously affects the accuracy of the platelet number detection result. To 200. Mu.L amikacin sulfate injection containing 3.8mg EDTAK 2 In the vacuum blood collection tube of the ultraviolet cap, part of platelets of the patient suffering from EDTA-dependent pseudothrombocytopenia still have aggregation, the volume and optical parameters of the aggregated platelets are close to those of white blood cells, the white blood cell count and analysis are easy to interfere, the number of the platelets and the white blood cells in a blood routine report and the analysis accuracy are reduced, and the treatment and diagnosis of the patient by doctors are delayed. The amikacin sulfate injection added into the purple head cap vacuum blood collection tube is a liquid preparation, has unstable property, needs to be temporarily added during use, needs to vacuumize the blood collection tube by using a syringe after the injection, has complex operation and is relatively less clinically used. At the same time, because the 3.2 percent of sodium citrate anticoagulant and amikacin injection are prepared by liquidThe preparation is 200 mu L, blood filled in the blood collection tube is diluted by sodium citrate anticoagulant and amikacin injection, after the whole blood mode detection is adopted, the detection result is required to be manually multiplied by a blood collection volume correction factor N for correction, and the blood collection volume correction factor N=V 2 /V 3 Wherein V is 2 V is the total volume of the solution in the blood collection tube after the vein blood is introduced into the vacuum blood collection tube 3 To introduce the actual volume of venous blood into the pre-diluted vacuum blood collection tube, V 3 ＝V 2 -0.2, said volume units being ml, the unit of the blood collection volume correction factor N being 1. Because the common vacuum blood collection tube has no obvious accurate scale, the total volume of the blood which is introduced into the vacuum blood collection tube cannot be accurately measured and interpreted, and especially when the total volume of the blood which is introduced into the vacuum blood collection tube is smaller than or exceeds the label scale mark of 2ml of the vacuum blood collection tube, the volume correction factor of the blood collection amount cannot be calculated, the number of blood platelets cannot be accurately corrected, the blue head cap vacuum blood collection tube containing 200 mu L of 3.2% sodium citrate anticoagulant is used, or the blue head cap vacuum blood collection tube containing 3.8mg EDTAK is added with 200 mu L of amikacin sulfate injection 2 The method for detecting the platelet number of the patient with EDTA-dependent pseudothrombocytopenia by using the whole blood mode of the full-automatic blood cell analyzer has the advantages of simple operation, good platelet number detection repeatability and poor accuracy.
Secondly, collecting peripheral blood, detecting blood routine by using a pre-dilution mode of a full-automatic blood cell analyzer, and obtaining the number of platelets. The specific operation flow is that a clean centrifuge tube is taken, 180 mu L of diluent special for blood cell analysis is added, after ring fingers of EDTA-dependent pseudothrombocytopenia patients are conventionally disinfected, 20 mu L of peripheral whole blood is collected by a micro blood collection straw, the peripheral whole blood is rapidly injected into the centrifuge tube containing 180 mu L of blood cell diluent, after a cover is covered, the mixture is uniformly mixed, after the mixture is kept still for 3 minutes, the blood convention is detected by using a pre-dilution mode of a full-automatic blood cell analyzer, and the detection result of the platelet number is obtained. Peripheral blood was collected, platelet count was measured using a pre-dilution mode, with Correcting the false aggregation of blood platelet, compared with vacuum blood collection tube with blue head cap containing 200. Mu.L of 3.2% sodium citrate anticoagulant and injection containing 3.8mg EDTAK and 200. Mu.L of amikacin sulfate 2 The vacuum blood collection tube with the ultraviolet cap of the anticoagulant has the advantage of more thorough, but with the time extension, a small part of patients still have platelet aggregation, and the analysis is because the special blood cell diluent contains EDTAK 2 Anticoagulant, EDTAK 2 The anticoagulant agent still induces the GPIIb glycoprotein conformation on the platelet membrane to change, exposes autoantigens (Ag) hidden in the GPIIb glycoprotein, and after the autoantigens (Ag) are recognized and combined by the anti-platelet autoantibodies (Ab) existing in a patient, the structure of the GPIIb/IIIa receptor on the platelet membrane is induced to change, the fibrinogen receptor is activated and exposed, and the fibrinogen receptor is directly connected with fibrinogen existing in blood, so that the platelets are aggregated in vitro, and the platelet aggregate is bigger and bigger along with the time extension. Because the same manufacturer, the same model, the same batch of products and the same barrel of micro blood collection straws are different to some extent, the micro blood collection straws are used, the accuracy of collecting the blood collection amount of peripheral whole blood is poor, and the repeatability is low. Peripheral whole blood is easily diluted and disturbed by tissue fluid and residual substances on the surface of skin, and collected blood cannot accurately and comprehensively represent the real state of blood in a patient. The standard peripheral blood collection has strict requirements on technical operation of operators, the sealing performance of the centrifuge tube for containing diluent is poor, water in the diluent is prepared in advance and volatilizes easily, dust easily enters the centrifuge tube, the detection of platelet number is interfered, the centrifuge tube for containing diluent is filled before daily use in clinical laboratory, the centrifuge tube for containing diluent is used, and peripheral blood collection cannot be popularized and used in nurses in hospitalization parts. Peripheral blood is collected, a blood routine is detected by using a pre-dilution mode of a full-automatic blood cell analyzer, and the method for obtaining the number of platelets of an EDTA-dependent pseudothrombocytopenia patient is guided due to various defects that peripheral blood is not represented by a physical state of venous blood, blood volume is collected by using a micro-blood collection straw, and part of platelets of the patient still can be aggregated in special diluent Peripheral blood is collected, blood is routinely detected using a pre-diluted mode of a fully automatic blood cell analyzer, and methods for obtaining platelet numbers are rarely used clinically.
Third, peripheral blood was collected and the number of platelets was counted visually using a microscope. The specific operation flow is that the tip of the ring finger of a patient is conventionally sterilized, 20 mu L of peripheral whole blood of the patient is collected by a micro blood collecting straw, the peripheral whole blood is injected into a test tube containing 380 mu L of 1% ammonium oxalate diluent, after the whole blood is completely hemolyzed, the whole blood is uniformly mixed for 1 minute, 1 drop of the platelet suspension after the uniform mixing is taken by a glass rod, the platelet suspension is filled into a counting pool of a blood cell counting plate, and is kept still for 10 to 15 minutes, so that the platelets sink, the high-power lens of a microscope is used for counting the number of platelets in five middle squares, namely four corners of a central large square and the center, and the number of platelets in the middle square is multiplied by 10 9 and/L is the number of platelets per liter of blood of the patient. The national clinical examination operation manual recommends that the phase microscope is used for visually counting platelets, so that the result is more accurate and the effect is better. The method for visually counting the number of the platelets by using a phase microscope is a traditional reference method for counting the platelets, but the method has high requirements on operators and equipment, a common laboratory is free of a phase microscope, the number of the platelets is visually counted by using a common optical microscope, the platelets which are easily interfered by impurities and dust in a platelet counting pool and stored in an ammonium oxalate diluent can be spontaneously dissolved along with the extension of the standing time, the counting result is reduced, the counting must be completed within 1 hour, the shorter the time is, the better the shorter the time is, the method for visually counting the number of the platelets by using the microscope is, the influence of technical errors and inherent errors is caused, the reproducibility of the method for visually counting the number of the platelets by using the microscope is poor, the same staff and the same sample is the same, and the variation coefficient of repeated counting before and after the number of the platelets reaches 10% -25% is not a reference method for counting the platelets. Meanwhile, as the same manufacturer, the same model, the same batch of products and the same barrel of micropipettes have certain differences, the accuracy of collecting peripheral whole blood by using the micropipettes is low, so that peripheral blood is collected, and the blood is visually counted by using a microscope The method for counting the number of the platelets has various defects, cannot meet the requirements of clinicians and patients on accuracy and timeliness of test reports, meanwhile, staff in clinical departments are used to use automatic equipment, the method for counting the number of the platelets by visual counting is contradicted by a purely manual operation method, the number of the platelets can only be counted by a microscope visual counting method, other blood cells cannot be counted, other parameters of the platelets cannot be measured, a complete blood routine report cannot be provided, and the possibility of blood collection of the patients exists, so that the method is rarely used in daily work.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a pre-dilution vacuum blood collection tube aiming at the defects of the prior art. The pre-dilution vacuum blood collection tube has simple structure, has accurate scale marks, is filled with 9mL sodium chloride injection or compound electrolyte injection, and is used for diluting venous whole blood and preventing anticoagulant (calcium ion chelating agent) from inducing exposure of autoantigen (Ag) in GPIIb glycoprotein on a platelet membrane; the venous blood is diluted in a large proportion to reduce the concentration of the blood coagulation factors in the blood solution in the pre-diluted vacuum blood collection tube, increase the distance between the blood coagulation factors, inhibit the mutual chained activation of the blood coagulation factors and prevent the coagulation of the blood; accurate and reliable venous blood volume collection is ensured through the precise scale on the blood collection tube; by using venous blood, dilution and interference of tissue fluid and skin surface residues on blood, platelet and coagulation factor activation are avoided when peripheral blood is collected; through the mode of vacuum blood sampling, the operation is simplified, and the pollution of open type collected blood to operators and the environment is prevented.
In order to solve the technical problems, the invention adopts the following technical scheme: the utility model provides a pre-diluted vacuum blood collection tube, includes the body and is used for sealing the tube stopper of body, the outside of tube stopper is provided with the pipe cap, and the body after the sealing is vacuum state, its characterized in that, be provided with on the outer wall of body from the scale mark that is used for annotating body volume, the scale value that the scale mark represents is from 9mL to 10.5mL, and minimum graduation value is 0.1mL, 9 mL's sodium chloride injection or compound electrolyte injection are equipped with in the body, the concentration of sodium chloride injection is 9g/L, the concentration of each raw materials in the compound electrolyte injection is: 5.26g/L of sodium chloride, 5.02g/L of sodium gluconate, 3.68g/L of sodium acetate, 0.37g/L of potassium chloride and 0.30g/L of magnesium chloride.
The pre-dilution vacuum blood collection tube is characterized in that the outer diameter of the tube body is 0.8 cm-2 cm, and the length of the tube body is 5 cm-35 cm.
The pre-dilution vacuum blood collection tube is characterized in that the outer diameter of the tube body is 1 cm-1.5 cm, and the length of the tube body is 10 cm-20 cm.
The pre-dilution vacuum blood collection tube is characterized in that the tube body is a glass tube or a PET plastic tube.
In addition, the invention also provides a method for measuring the platelet number of the EDTA-dependent pseudothrombocytopenia patient by utilizing the pre-dilution vacuum blood collection tube and matching with a full-automatic blood cell analyzer, which is characterized by comprising the following steps:
Step one, binding a tourniquet on the arm of an EDTA-dependent pseudothrombocytopenia patient, searching a vein, conventionally sterilizing, penetrating one end needle head of a blood taking needle into the vein of the patient, penetrating the other end needle head of the blood taking needle into a tube plug of a pre-dilution vacuum blood taking tube after blood return, introducing vein blood into the pre-dilution vacuum blood taking tube, rapidly pulling out the needle head of the blood taking needle inserted on the tube plug, rapidly reversing for 5-8 times, uniformly mixing to obtain diluted blood,
detecting the diluted blood obtained in the first step by using a full-automatic blood cell analyzer to obtain a detection value of the number of platelets, wherein the detection value of the number of platelets is multiplied by a blood sampling volume correction factor U to obtain a true value of the number of platelets; the full-automatic blood cell analyzer adopts a pre-dilution mode for detection, and the blood sampling volume correction factor U=V 1 V, where V 1 To introduce a standard volume of venous blood into the pre-diluted vacuum blood collection tube, V 1 The value of (1) is 1mL; v is the actual volume of the vein blood introduced into the pre-diluted vacuum blood collection tube, and the value of V meets the following conditions: v is more than or equal to 0.5mL and less than or equal to 1.5mL.
The method is characterized in that 1mL of venous blood is introduced into the pre-diluted vacuum blood collection tube in the first step.
Compared with the prior art, the invention has the following advantages:
1. compared with the existing special vacuum blood collection tube, the pre-diluted vacuum blood collection tube is directly added with the sodium chloride injection or the compound electrolyte injection for diluting blood, does not need secondary addition, is directly added into the vacuum blood collection tube, and avoids secondary operation; meanwhile, the blood collection tube is provided with accurate scale values, so that a detector can conveniently read the addition amount of blood, calculate the dilution ratio of the blood and measure the accurate platelet detection value. The pre-injected 9ml sodium chloride injection or compound electrolyte injection is used for diluting venous blood, does not need to add any anticoagulant (calcium ion chelating agent) or antagonist such as medicine and the like, can prevent EDTA-dependent pseudothrombocytopenia patients from platelet aggregation induced by the anticoagulant (calcium ion chelating agent) in vitro, corrects platelet aggregation induced by the anticoagulant, and detects the generated platelet number error, ensures the accuracy and reliability of the platelet number during routine blood detection, and simultaneously ensures the accuracy and reliability of the leukocyte number and classification result.
2. The pre-dilution vacuum blood collection tube is mainly used for preventing the phenomenon of platelet aggregation induced by an anticoagulant (a calcium ion chelating agent) in vitro when blood is collected from platelets of an EDTA-dependent pseudothrombocytopenia patient, ensuring that the accurate platelet number is obtained when a full-automatic blood cell analyzer pre-dilution mode is used for detecting blood convention, and the whole blood collection process is simple to operate and easy to popularize.
3. According to the invention, the volume of the sodium chloride injection or the compound electrolyte injection is precisely controlled, so that the sodium chloride injection or the compound electrolyte injection is used as a diluent to dilute venous blood, 0.5-1.5 mL of blood is introduced into a pre-diluted vacuum blood collection tube at one time, a pre-dilution mode of a full-automatic blood cell analyzer is adopted after the pre-diluted blood is uniformly mixed, diluted blood is detected, a detection result is multiplied by a blood collection volume correction factor U, a true value of the number of platelets can be obtained, the accuracy of the detection result is ensured, and one rough blood collection is realized, so that the accurate number of platelets can be obtained, the technical requirements of the blood collection personnel are reduced, the blood collection precision can be improved, but the volume of each blood collection of the blood collection personnel is 1mL, the blood collection volume corresponding to the blood collection volume is 1, and the detection value of the number of platelets of the full-automatic blood cell analyzer is equal to the true value of the number of platelets.
4. The invention adopts the pre-dilution mode detection of the full-automatic blood cell analyzer, has good repeatability, high speed and high accuracy of platelet number detection results, is easy to control quality, meets the requirements of medical inspection professional quality specifications, and meets the accurate and timely service requirements of clinical patients on inspection reports.
5. The invention collects and dilutes venous blood by utilizing the pre-dilution vacuum blood collection tube, uses the full-automatic blood cell analyzer to measure the number of platelets, simultaneously, the analyzer can also detect all blood routine items to obtain comprehensive blood routine information, uses sodium chloride injection or compound electrolyte injection as diluent, has low detection cost, is nontoxic and harmless to the environment, and can carry out innocuous treatment by detecting the residual blood through a chlorine-containing disinfectant soaking mode.
6. The invention realizes the closed blood collection by adopting the vein blood collection mode of the vacuum blood collection tube, does not pollute the environment and operators, and meets the infection control requirement; the method has no special requirements on personnel equipment, is easy to accept by staff, corrects EDTA-dependent pseudothrombocytopenia patients, completely and thoroughly gathers platelets induced by anticoagulant (calcium ion chelating agent) in vitro, and ensures the accuracy and reliability of the platelet number during routine blood detection, so that the measurement method can be truly used clinically; meanwhile, the pre-diluted vacuum blood collection tube can be processed into commodities, can be widely popularized and used, and particularly can be used in nurses in hospitalization departments, so that the working efficiency of clinical departments is greatly improved, and the time for issuing blood routine inspection reports is shortened.
The technical scheme of the invention is further described in detail through the drawings and the embodiments.
Drawings
Fig. 1 is a schematic structural view of a pre-diluted vacuum blood collection tube according to the present invention.
Fig. 2 is a microscopic image of a blood smear prepared by the blood sampling method of comparative example 1 for patient 1.
Fig. 3 is a microscopic image of a blood smear prepared by the blood sampling method of comparative example 2 for patient 1.
Fig. 4 is a microscopic image of a blood smear prepared by the blood sampling method of comparative example 3 for patient 1.
Fig. 5 is a microscopic image of a blood smear prepared by the blood sampling method of example 1 for patient 1. Reference numerals illustrate:
1-a tube body; 2, a pipe cap; 3-a pipe plug;
4-scale marks.
Detailed Description
Example 1
As shown in fig. 1, the pre-diluted vacuum blood sampling tube comprises a tube body 1 and a tube plug 3 for sealing the tube body 1, a tube cap 2 is arranged on the outer side of the tube plug 3, the sealed tube body 1 is in a vacuum state, a scale mark 4 for marking the volume of the tube body 1 is arranged on the outer wall of the tube body 1, the scale mark 4 represents a scale value from 9mL to 10.5mL, the minimum scale value is 0.1mL, 9mL of sodium chloride injection or compound electrolyte injection is filled in the tube body 1, and the concentration of the sodium chloride injection is 9g/L.
In this embodiment, the sodium chloride injection is used as the diluent, the detection cost is low, no description is given in the prior art about the sodium chloride injection, the sodium chloride injection can be used for diluting blood in a vacuum blood collection tube, the result of blood cell count after dilution is stable within 4 hours, and the vacuum blood collection tube after detection can be repeatedly used after cleaning, so that the resources are saved and the environment is protected.
In this embodiment, the outer diameter of the tube body 1 is 1.3cm, and the length is 16cm.
In this embodiment, the tube body 1 is a glass tube, which has low cost and is convenient for industrialization.
Example 2
In this embodiment, the difference from embodiment 1 is that: the outer diameter of the pipe body 1 is 2cm, and the length is 5cm.
In this embodiment, the structure and connection relation of the rest are the same as those of embodiment 1.
Example 3
In this embodiment, the difference from embodiment 1 is that: the outer diameter of the pipe body 1 is 1.25cm, and the length is 15cm.
In this embodiment, the structure and connection relation of the rest are the same as those of embodiment 1.
Example 4
In this embodiment, the difference from embodiment 1 is that: the tube body 1 is provided with 9ml of compound electrolyte injection, and the concentration of each raw material in the compound electrolyte injection is as follows: 5.26g/L of sodium chloride, 5.02g/L of sodium gluconate, 3.68g/L of sodium acetate, 0.37g/L of potassium chloride and 0.30g/L of magnesium chloride.
In this embodiment, the external diameter of body 1 is 0.8cm, and length is 35cm, and the length of body 1 is high, and the distance is sparse between the scale mark, conveniently looks over the scale value.
In this embodiment, the compound electrolyte injection may also be used as a diluent, and the accuracy of the obtained detection result is more accurate than that obtained by the existing detection method.
In this embodiment, the tube body 1 is a plastic tube, the bottom of the plastic tube is adopted to make a planar structure, the plastic tube overcomes the defect that the concave liquid surface is provided on the liquid surface when the liquid is contained in the glass tube, and when the blood is introduced into the pre-diluted vacuum blood collection tube, the diluted blood sample is red, and the lowest part of the concave liquid surface can not be distinguished, so that the read data has constant negative deviation.
In this embodiment, the structure and connection relation of the rest are the same as those of embodiment 1.
Example 5
In this embodiment, the difference from embodiment 4 is that: the outer diameter of the pipe body 1 is 1cm, and the length is 20cm.
In this embodiment, the structure and connection relation of the rest are the same as those of embodiment 1.
Example 6
In this embodiment, the difference from embodiment 5 is that: the outer diameter of the pipe body 1 is 1.5cm, and the length is 10cm.
In this embodiment, the structure and connection relation of the rest are the same as those of embodiment 1.
Example 7
A method for measuring the number of platelets using the pre-diluted vacuum blood collection tube of example 1 in combination with a fully automatic blood cell analyzer, the method comprising the steps of:
firstly, binding a tourniquet on the arm of an EDTA-dependent pseudothrombocytopenia patient, searching a vein, conventionally sterilizing, penetrating a needle head at one end of a blood taking needle into the vein of the patient, penetrating a tube plug 3 of a pre-diluted vacuum blood taking tube with the needle head at the other end of the blood taking needle after blood return, introducing 1ml of vein blood into the pre-diluted vacuum blood taking tube, rapidly pulling out the needle head of the blood taking needle inserted on the tube plug 3, rapidly reversing and uniformly mixing for 5 times, and obtaining diluted blood;
step two, using a full-automatic blood cell analyzer to select a pre-dilution mode, detecting the diluted blood obtained in the step one to obtain a detection value of the number of platelets, multiplying the detection value of the number of platelets by a blood sampling volume correction factor U, and finally obtaining a true value of the number of platelets; the full-automatic blood cell analyzer adopts a pre-dilution mode, and the blood sampling volume correction factor U=V 1 V, where V 1 To introduce a standard volume of venous blood into the pre-diluted vacuum blood collection tube, V 1 1mL, V is the actual volume of the blood flowing into the vein of the pre-diluted vacuum blood collection tube, V is 1mL, the unit of the blood collection volume correction factor U is 1, the value of U is 1 in comparison with the table 1, and the detection value of the number of the blood platelets is the true value of the blood platelets.
TABLE 1 blood collection volume correction factor U and V relationship correspondence table
| V | 0.5 | 0.6 | 0.7 | 0.8 | 0.9 | 1 | 1.1 | 1.2 | 1.3 | 1.4 | 1.5 |
| U | 2 | 1.67 | 1.43 | 1.25 | 1.11 | 1 | 0.91 | 0.83 | 0.77 | 0.71 | 0.67 |
In this embodiment, when venous blood passes through the pre-dilution vacuum blood collection tube, a detector should strictly observe the reading convention, when the blood collection tube is required to be placed vertically in the reading, eyes look up the scale marks, when the added blood makes the upper surface of the solution in the blood collection tube tangential to the scale marks, the blood collection needle inserted on the rubber plug of the blood collection tube is pulled out rapidly, blood collection is stopped, mixing is reversed, accuracy of blood collection amount is guaranteed, and accuracy of platelet number detection results is further guaranteed.
Meanwhile, when the glass tube is used as the pre-dilution vacuum blood collection tube, after blood is introduced into the pre-dilution vacuum blood collection tube, the diluted blood sample is red, the lowest part of the concave liquid surface cannot be distinguished, and if a 10ml scale mark is used as a stop line of the venous blood collection amount, the blood collection amount can have constant negative deviation, which is less than 1ml. The highest part of the concave liquid surface can only be observed on the liquid surface after blood collection, and experiments prove that the liquid surface after blood collection of the pre-dilution vacuum blood collection tube is tangential to 10.2ml scale marks to ensure the accurate blood collection amount of 1ml.
The method for measuring platelets of the embodiment can also be used for measuring other items in blood routine and is accurate in measurement.
Example 8
A method for measuring the number of platelets using the pre-diluted vacuum blood collection tube of example 1 in combination with a fully automatic blood cell analyzer, the method comprising the steps of:
step one, tying a tourniquet on the arm of a patient with pseudo thrombocytopenia, searching a vein, conventionally sterilizing, penetrating a needle head at one end of a blood taking needle into the vein of the patient, penetrating a tube plug 3 of a pre-diluted vacuum blood taking tube with the needle head at the other end of the blood taking needle after blood return, introducing 1.2mL of vein blood into the pre-diluted vacuum blood taking tube, rapidly pulling out the needle head of the blood taking needle inserted on the tube plug 3, rapidly reversing and uniformly mixing for 5 times to obtain diluted blood,
step two, using a full-automatic blood cell analyzer to select a pre-dilution mode, and detecting the diluted blood obtained in the step one to obtain a detection value of the number of platelets, wherein the detection value of the number of platelets is multiplied byA blood sampling volume correction factor U, and finally obtaining a true value of the number of platelets; the blood analyzer adopts a pre-dilution mode to detect, and the blood sampling volume correction factor U=V 1 V, where V 1 To introduce a standard volume of venous blood into the pre-diluted vacuum blood collection tube, V 1 1mL, V is the actual volume of the venous blood introduced into the pre-diluted vacuum blood collection tube, V is 1.2mL, the unit of the blood collection volume correction factor U is 1, and the value of U is 0.83 in comparison table 1.
When the pre-dilution vacuum blood collection tube needs to be placed vertically to collect blood, eyes look at the scale marks in a head-on manner, when the upper surface of the solution in the blood collection tube is tangent to the scale marks by blood to be added, a blood collection needle inserted into a rubber plug of the blood collection tube is pulled out rapidly, blood collection is stopped, accuracy of blood collection amount is guaranteed, and accuracy of platelet number detection results is further guaranteed.
The method for measuring platelets of the embodiment can also be used for measuring other items in blood routine and is accurate in measurement.
Example 9
A method for measuring the number of platelets using the pre-diluted vacuum blood collection tube of example 1 in combination with a fully automatic blood cell analyzer, the method comprising the steps of:
step one, binding a tourniquet on the arm of an EDTA-dependent pseudothrombocytopenia patient, searching a vein, conventionally sterilizing, penetrating one end needle head of a blood taking needle into the vein of the patient, penetrating the other end needle head of the blood taking needle into a tube plug 3 of a pre-dilution vacuum blood taking tube after blood return, introducing 0.8mL of vein blood into the pre-dilution vacuum blood taking tube, rapidly pulling out the needle head of the blood taking needle inserted on the tube plug 3, rapidly reversing and uniformly mixing for 5 times to obtain diluted blood,
Step two, a full-automatic blood cell analyzer is utilized, a pre-dilution mode is selected, the diluted blood obtained in the step one is subjected to blood routine detection, a detection value of the number of blood platelets is obtained, the detection value of the number of blood platelets is multiplied by a blood sampling volume correction factor U, and finally a true value of the number of blood platelets is obtained; the full-automatic blood cell analyzer adopts pre-preparationDilution mode detection, the blood collection volume correction factor u=v 1 V, where V 1 To introduce a standard volume of venous blood into the pre-diluted vacuum blood collection tube, V 1 1mL, V is the actual volume of the venous blood introduced into the pre-diluted vacuum blood collection tube, V is 0.8mL, the unit of the blood collection volume correction factor U is 1, and the value of U is 1.25 according to the comparison table 1.
When the pre-dilution vacuum blood collection tube needs to be placed vertically to collect blood, eyes look at the scale marks in a head-on manner, when the upper surface of the solution in the blood collection tube is tangent to the scale marks by blood to be added, a blood collection needle inserted into a rubber plug of the blood collection tube is pulled out rapidly, blood collection is stopped, accuracy of blood collection amount is guaranteed, and accuracy of platelet number detection results is further guaranteed.
The method for measuring platelets of the embodiment can also be used for measuring other items in blood routine and is accurate in measurement.
The number of platelets in blood was measured for three patients at the same time by the methods of example 7, example 8 and example 9, respectively, and the test results are shown in table 2.
Table 2 test results for three patients for the number of platelets in blood using the methods of example 7, example 8 and example 9, respectively
As is clear from Table 2, the same patient has substantially the same number of platelets detected by the methods of examples 7, 8 and 9, which indicates that the results of detecting the number of platelets in the full-automatic blood cell analyzer for different blood collection amounts are accurate and reproducible by correcting the blood collection amount volume correction factor U.
Example 10
A method for measuring the number of platelets using the pre-diluted vacuum blood collection tube of example 1 in combination with a fully automatic blood cell analyzer, the method comprising the steps of:
firstly, binding a tourniquet on the arm of an EDTA pseudo thrombocytopenia patient, searching a vein, conventionally sterilizing, penetrating a needle head at one end of a blood taking needle into the vein of the patient, penetrating a tube plug 3 of a pre-diluted vacuum blood taking tube through the needle head at the other end of the blood taking needle after blood return, introducing 0.5mL of vein blood into the pre-diluted vacuum blood taking tube, rapidly pulling out the needle head of the blood taking needle inserted on the tube plug 3, and rapidly reversing and uniformly mixing for 8 times to obtain diluted blood;
Step two, using a full-automatic blood cell analyzer to select a pre-dilution mode, detecting the diluted blood obtained in the step one to obtain a detection value of the number of platelets, multiplying the detection value of the number of platelets by a blood sampling volume correction factor U, and finally obtaining a true value of the number of platelets;
the blood analyzer adopts a pre-dilution mode, and the blood sampling volume correction factor U=V 1 V, where V 1 To introduce a standard volume of venous blood into the pre-diluted vacuum blood collection tube, V 1 The blood sampling volume correction factor U is 1, the value of U is 1 in comparison with the table 1, and the platelet number detection value of the full-automatic blood cell analyzer is the true value of the platelet number.
When the pre-dilution vacuum blood collection tube needs to be placed vertically to collect blood, eyes look at the scale marks in a head-on manner, when the upper surface of the solution in the blood collection tube is tangent to the scale marks by blood to be added, a blood collection needle inserted into a rubber plug of the blood collection tube is pulled out rapidly, blood collection is stopped, accuracy of blood collection amount is guaranteed, and accuracy of platelet number detection results is further guaranteed.
The method for measuring platelets of the embodiment can also be used for measuring other items in blood routine and is accurate in measurement.
Example 11
A method for measuring the number of platelets using the pre-diluted vacuum blood collection tube of example 1 in combination with a fully automatic blood cell analyzer, the method comprising the steps of:
firstly, binding a tourniquet on the arm of an EDTA pseudo thrombocytopenia patient, searching a vein, conventionally sterilizing, penetrating a needle head at one end of a blood taking needle into the vein of the patient, penetrating a tube plug 3 of a pre-diluted vacuum blood taking tube through the needle head at the other end of the blood taking needle after blood return, introducing 1.5mL of vein blood into the pre-diluted vacuum blood taking tube, rapidly pulling out the needle head of the blood taking needle inserted on the tube plug 3, and rapidly reversing and uniformly mixing for 8 times to obtain diluted blood;
step two, using a full-automatic blood cell analyzer to select a pre-dilution mode, detecting the diluted blood obtained in the step one to obtain a detection value of the number of platelets, multiplying the detection value of the number of platelets by a blood sampling volume correction factor U, and finally obtaining a true value of the number of platelets; the blood analyzer adopts a pre-dilution mode, and the blood sampling volume correction factor U=V 1 V, where V 1 To introduce a standard volume of venous blood into the pre-diluted vacuum blood collection tube, V 1 The blood sampling volume correction factor U is 1, the value of U is 1 in comparison with the table 1, and the platelet number detection value of the full-automatic blood cell analyzer is the true value of the platelet number.
When the pre-dilution vacuum blood collection tube needs to be placed vertically to collect blood, eyes look at the scale marks in a head-on manner, when the upper surface of the solution in the blood collection tube is tangent to the scale marks by blood to be added, a blood collection needle inserted into a rubber plug of the blood collection tube is pulled out rapidly, blood collection is stopped, accuracy of blood collection amount is guaranteed, and accuracy of platelet number detection results is further guaranteed.
The method for measuring platelets of the embodiment can also be used for measuring other items in blood routine and is accurate in measurement.
Comparative example 1
Binding tourniquet on arm of EDTA pseudo thrombocytopenia patient, searching vein blood vessel, conventionally sterilizing skin, penetrating needle head of one end of blood taking needle into blood vessel of patient, and penetrating needle head of the other end of blood taking needle into EDTAK after blood return 2 Anticoagulation tube, and then collect the blood to a concentration of 3.8mg EDTAK 2 2ml of marking line of the vacuum blood collection tube of the anticoagulant, then rapidly pulling out the blood collection needle head inserted on the tube plug, rapidly reversing and uniformly mixing blood in the vacuum blood collection tube for 5 times, then detecting the blood by adopting a whole blood mode on a blood analyzer, and testing the number of blood platelets in the blood of a patient to obtain a blood platelet detection value of 21 EDTA-dependent pseudothrombocytopenia patient blood platelet correction method 1.
Comparative example 2
Binding tourniquet on arm of patient with EDTA pseudo thrombocytopenia, searching vein blood vessel, sterilizing skin, penetrating needle head at one end of blood taking needle into blood vessel of patient, penetrating needle head at the other end of blood taking needle into vacuum blood taking tube with 200 μL of 3.2% sodium citrate anticoagulant, collecting blood at 2ml mark line of anticoagulant tube, rapidly pulling out blood taking needle head inserted on blood taking tube rubber plug, rapidly reversing and mixing blood in vacuum blood taking tube for 5 times, detecting blood by whole blood mode on blood analyzer, testing platelet number detection value in blood of patient, multiplying detection value of platelet number with blood volume correction factor 1.11, obtaining platelet true value of 21 cases of blood taking correction method 2 of EDTA-dependent pseudo thrombocytopenia patient.
Comparative example 3
Binding tourniquet on arm of patient with EDTA pseudo thrombocytopenia, searching vein blood vessel, conventionally sterilizing skin, penetrating needle head of one end of blood taking needle into blood vessel of patient, and penetrating needle head of the other end of blood taking needle into blood vessel containing 3.8mg EDTAK after blood return 2 Anticoagulation tube, EDTAK 2 200 mu L of amikacin sulfate injection is added in the anticoagulation tube in advance, and then the blood is collected into EDTAK 2 And (3) at the position of a 2ml mark line of the anticoagulation tube, rapidly pulling out a blood sampling needle inserted on a tube plug, rapidly reversing and uniformly mixing blood in the vacuum blood sampling tube for 5 times, detecting the blood by adopting a whole blood mode on a blood analyzer, and testing a platelet number detection value in the blood of a patient, wherein the detection value is multiplied by a blood sampling volume correction factor of 1.11 to obtain a platelet true value of the platelet correction method 3 of 21 EDTA-dependent pseudothrombocytopenia patients.
The blood of 21 patients were tested for platelets by the methods of example 7, comparative example 1, comparative example 2 and comparative example 3 simultaneously, and the test results of the number of platelets are shown in table 3.
Table 3 platelet count measurements for 21 patients using the methods of example 7, comparative example 1, comparative example 2 and comparative example 3
As can be seen from table 3 in combination with fig. 2, 3, 4 and 5, EDTA-dependent pseudothrombocytopenia phenomenon, which is a phenomenon of in vitro platelet aggregation induced by an anticoagulant (calcium ion chelating agent) at room temperature, not only EDTA alone may induce, but also other anticoagulants such as sodium citrate may cause platelet aggregation, and it has been reported that anticoagulants such as sodium oxalate, dextrose citrate, heparin (calcium ion chelating agent) may also cause platelet aggregation, resulting in a pseudo-decrease in the number of platelets. Among them, EDTA has the strongest ability to chelate calcium ions, so that the induced platelet aggregation is the most serious; the ability of the sodium citrate anticoagulant to chelate calcium ions is poorer than that of EDTA, the effect of the sodium citrate anticoagulant tube on correcting the pseudo-aggregation of the platelets is ideal than that of the EDTA anticoagulant tube, but the platelets still have slight aggregation, the aggregation degree is obviously reduced than that of the EDTA anticoagulant aggregation, but the platelets visible at the tail part of the smear are aggregated in a large block, and the accuracy of the number of the platelets is affected; EDTAK containing 3.8mg and 200 μl amikacin injection was added 2 The vacuum blood collection tube of the ultraviolet cap of the anticoagulant has unsatisfactory effect on the aggregation correction of pseudo thrombocytopenia, has poor effect on inhibiting the aggregation of thrombocytes, and has the condition that the patient still has the aggregation of thrombocytes, and the amikacin sulfate used in the test has high concentration of 10mg/L and cannot effectively correct the thrombocytes induced by EDTA in vitro Platelet aggregation, which is also not ideal in correcting anticoagulant-induced platelet aggregation effects using amikacin sulfate-added EDTA anticoagulant tubes, corrects in vitro anticoagulant (calcium ion chelator) -induced platelet pseudo-aggregation patient-dependent, not dose-dependent. Vacuum blood collection tube containing 200 μl of 3.2% sodium citrate anticoagulant, or containing 3.8mg of EDTAK added with 200 μl of amikacin sulfate injection 2 Venous blood is collected by a vacuum blood collection tube of an anticoagulant, and an anticoagulant is used for correcting and detecting the blood platelet aggregation of EDTA-dependent pseudothrombocytopenia patients, wherein the platelet aggregation is induced by the anticoagulant (calcium ion chelating agent) in vitro, the defects of incomplete platelet aggregation in vitro and inaccurate platelet number detection exist, in order to better achieve the effect of correcting the platelet aggregation in vitro, the literature suggests that an anticoagulant sample of the patients is subjected to 37 ℃ heat preservation, the autoantibody (Ab) of the anti-platelet is prevented from being combined with autoantigen (Ag) in glycoprotein GPIIb at room temperature, but the temperature of the blood sample is always kept at 37 ℃ from a ward to a clinical laboratory in daily work, the operation is difficult, and the vacuum blood collection tube containing 200 mu L of 3.2% sodium citrate anticoagulant or the 3.8mg EDTAK containing 200 mu L of amikacin sulfate injection is possibly influenced 2 The vacuum blood collection tube of the anticoagulant has the effect of correcting the pseudo-aggregation of the blood platelets. The use of the pre-diluted vacuum blood collection tube of the invention for assisting in preparing a detection blood sample for detecting the platelets of a patient suffering from pseudo thrombocytopenia prevents the anticoagulant from inducing the exposure and activation of autoantigens (Ag) in platelet membrane glycoprotein GPIIb from the source because the anticoagulant is not used; simultaneously collecting venous blood, preventing tissue factors in tissue fluid from activating platelets and coagulation factors and diluting blood; the method of 1:10 large-scale dilution is used for increasing the distance between the blood coagulation factors, blocking the chain activation between the blood coagulation factors and playing an anticoagulation role; meanwhile, the concentration of platelet autoantibodies (Ab) is reduced by dilution, and the reaction strength with autoantigens (Ag) in platelet membrane glycoprotein GPIIb is weakened; finally, the activation expression of GPIIb/IIIa receptor fibrinogen receptor on the platelet mould is thoroughly prevented, and the aggregation phenomenon of platelets induced by anticoagulant in vitro is blocked. The accurate and controllable blood sampling amount is ensured through the accurate scale, the operation is simple, safe and convenient; the pre-dilution mode of the full-automatic blood analyzer ensures the repeated reliability of the platelet number detection result; the blood sampling method of the pre-dilution vacuum blood sampling tube has the advantages of simple operation, reliable platelet number detection, good repeatability, low cost and good economy, has sufficient commercialized conditions, completely eliminates the aggregation of platelets in vitro for patients with EDTA-dependent pseudothrombocytopenia, has accurate and reliable platelet number when detecting blood conventionally, has no requirement on personnel and equipment, and is convenient for large-scale popularization and use.
The above description is only of the preferred embodiments of the present invention, and is not intended to limit the present invention. Any simple modification, variation and equivalent variation of the above embodiments according to the technical substance of the invention still fall within the scope of the technical solution of the invention.
Claims (4)
1. The utility model provides a pre-diluted vacuum blood collection tube, includes body (1) and is used for sealing pipe stopper (3) of body (1), the outside of pipe stopper (3) is provided with pipe cap (2), and body (1) after the seal is vacuum state, a serial communication port, be provided with on the outer wall of body (1) from scale mark (4) that are used for annotating body (1) volume, scale mark (4) represent the scale mark value from 9mL to 10.5mL, minimum scale mark value is 0.1mL, be equipped with 9mL sodium chloride injection or compound electrolyte injection in body (1), the concentration of sodium chloride injection is 9g/L, the concentration of each raw materials in the compound electrolyte injection is: 5.26g/L of sodium chloride, 5.02g/L of sodium gluconate, 3.68g/L of sodium acetate, 0.37g/L of potassium chloride and 0.30g/L of magnesium chloride;
the method for measuring the platelet number by utilizing the pre-diluted vacuum blood collection tube and matching with a full-automatic blood cell analyzer comprises the following steps:
step one, introducing vein blood into a pre-diluted vacuum blood collection tube, and then rapidly reversing for 5-8 times and uniformly mixing to obtain diluted blood;
Detecting the diluted blood obtained in the first step by using a full-automatic blood cell analyzer to obtain a detection value of the number of platelets, wherein the detection value of the number of platelets is multiplied by a blood sampling volume correction factor U to obtain a true value of the number of platelets; the full-automatic blood cell analyzer adopts a pre-dilution mode to detect, and the blood sampling volume correction factor U=V1/V, wherein V1 is the standard volume of the intravenous blood introduced into the pre-dilution vacuum blood sampling tube, and the value of V1 is 1mL; v is the actual volume of the vein blood introduced into the pre-diluted vacuum blood collection tube, and the value of V meets the following conditions: v is more than or equal to 0.5mL and less than or equal to 1.5mL.
2. A pre-diluted vacuum blood collection tube according to claim 1, wherein the tube body (1) has an outer diameter of 0.8 cm-2 cm and a length of 5 cm-35 cm.
3. A pre-diluted vacuum blood collection tube according to claim 2, wherein the tube body (1) has an outer diameter of 1cm to 1.5cm and a length of 10cm to 20cm.
4. A pre-diluted vacuum blood collection tube according to claim 1, characterized in that the tube body (1) is a glass tube or a PET plastic tube.
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| CN108982382B (en) * | 2018-07-17 | 2021-05-18 | 厦门市计量检定测试院 | Calibration method of on-line total chromium analyzer |
| CN109813884A (en) * | 2019-03-25 | 2019-05-28 | 中国动物卫生与流行病学中心 | A kind of perlsucht gamma interferon quickly detects product |
| CN109998561B (en) * | 2019-05-15 | 2022-01-28 | 广西中医药大学附属瑞康医院 | Disposable blood coagulation vacuum blood collection tube |
| CN113470770B (en) * | 2021-06-30 | 2022-03-01 | 北京大学第三医院（北京大学第三临床医学院） | Platelet detection system based on error prediction model |
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| JPH09192119A (en) * | 1996-01-12 | 1997-07-29 | Sekisui Chem Co Ltd | Vacuum blood-collecting tube |
| CN202489959U (en) * | 2012-02-28 | 2012-10-17 | 徐大勇 | Vacuum blood collection tube for blood routine examination |
| CN106404636A (en) * | 2016-08-31 | 2017-02-15 | 成都瑞琦科技实业股份有限公司 | Tube and blood collection tube capable of avoiding appearance of EDTA dependent pseudothrombocytopenia (PTCP), and preparation method thereof |
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| WO2012075407A2 (en) * | 2010-12-02 | 2012-06-07 | Becton, Dickinson And Company | Blood collection devices containing blood stabilization agent |
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| JPH09192119A (en) * | 1996-01-12 | 1997-07-29 | Sekisui Chem Co Ltd | Vacuum blood-collecting tube |
| CN202489959U (en) * | 2012-02-28 | 2012-10-17 | 徐大勇 | Vacuum blood collection tube for blood routine examination |
| CN106404636A (en) * | 2016-08-31 | 2017-02-15 | 成都瑞琦科技实业股份有限公司 | Tube and blood collection tube capable of avoiding appearance of EDTA dependent pseudothrombocytopenia (PTCP), and preparation method thereof |
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