CN101644706A - Semi-quantitative sperm concentration rapid self-test kit, prepration method and using method thereof - Google Patents
Semi-quantitative sperm concentration rapid self-test kit, prepration method and using method thereof Download PDFInfo
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- CN101644706A CN101644706A CN200910081425A CN200910081425A CN101644706A CN 101644706 A CN101644706 A CN 101644706A CN 200910081425 A CN200910081425 A CN 200910081425A CN 200910081425 A CN200910081425 A CN 200910081425A CN 101644706 A CN101644706 A CN 101644706A
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Abstract
The invention discloses a semi-quantitative sperm concentration rapid self-test kit, a prepration method and a using method thereof, the semi-quantitative sperm concentration rapid self-test kit comprises a sperm collection box, cell staining solution, physiological saline and a test board, wherein, the sperm collection box consists of a plastic collection box and a sperm liquefying agent, the test board comprises a housing and a substrate, a test hole and a control hole are formed in the center of the housing, water-absorbing paper, a filter membrane and a standard color unit are arranged onthe substrate, and the color of the standard color unit is the color of the test results of adding sperm samples with the concentration of 5 million, 10 million, 20 million, 30 million, 40 million, 60million, 80 million and 100 million/ml into the cell staining solution. The semi-quantitative sperm concentration rapid self-test kit has the advantages that the semi-quantitative sperm concentrationrapid self-test kit can rapidly and accurately detect the concentration of sperm and further diagnose whether a man can produce offspring or not, compared with the traditional diagnosis method, the semi-quantitative sperm concentration rapid self-test kit can save a lot of time, the use is simple, the semi-quantitative sperm concentration rapid self-test kit can be purchased at a pharmacy and used at home, thereby avoiding the feeling of shyness of a user.
Description
Technical field
The present invention relates to a kind of in-vitro diagnosis class medicine equipment, specifically is a kind of sxemiquantitative rapid self-inspection kit that is applicable to mankind spermatozoon concentration.
Background technology
The fertility expert report, (no contraceptives) can not be conceived in 1 year for about in the world 15% Mr. and Mrs, and 40% in these cases are because male factor causes.Also have Andriatrics Dept. expert report, the incidence of disease of male sterility is approximately 6-12%.Above-mentioned report, the crowd that male sterility is described is a sizable crowd.
The main method of current diagnosis male sterility is the microscopically diagnosis of seminal fluid, and promptly sperm morphological detects, and this also is the goldstandard of male sterility diagnosis.But there are many shortcomings in this method, such as: because Chinese traditional concept is shyly gone the disease of hospital therapy male sterility aspect, thereby has been incured loss through delay treatment.Even gone to hospital, owing to the condition of getting sperm is limited, be subjected to the restriction of environment, method, time, be difficult for obtaining sample.It is more loaded down with trivial details to go to a hospital to see a doctor, must be through a series of programs such as registering, prescribe.
Summary of the invention
The objective of the invention is in order to overcome the shortcoming of prior art, provide a kind of and can operate at home, quick, easy to use, low-cost semi-quantitative sperm concentration rapid self-inspection kit.
Second purpose of the present invention provides a kind of preparation and using method of semi-quantitative sperm concentration rapid self-inspection kit.
For achieving the above object, the present invention includes seminal fluid collecting box, cell dyeing liquid, physiological saline and breadboard, described seminal fluid collecting box collects box by plastics and the seminal fluid liquefier is formed, described breadboard comprises shell, substrate, shell and substrate are that rectangular PVC plastics are made, and there is a test hole in shell central authorities, and thieving paper, filtering membrane and standard colour code are arranged on the described substrate, thieving paper and filtering membrane are positioned at the substrate top, the test hole below of shell; Thieving paper is positioned at the filtering membrane below; The standard colors mark is around test hole on the shell, and standard colors target color is the color that 5,000,000,1,000 ten thousand, 2,000 ten thousand, 3,000 ten thousand, 4,000 ten thousand, 6,000 ten thousand, 8,000 ten thousand, 10,000 ten thousand/ml concn sample of sperm adds the cell dyeing liquid test findings.Described filtering membrane is the hydrophilic glass tunica fibrosa, and pore diameter range 1-30 micron, size dimension are 10 millimeters * 10 millimeters; Described thieving paper is lignocellulose paper, and its size dimension is consistent with filtering membrane.Described seminal fluid liquefier can be enzyme preparation such as bromelain, pronase or chymotrypsin, and non-enzyme preparation such as dithiothreitol (DTT), working concentration is 1-5mg/ml.Described cell dyeing liquid can be acid, alkalescence and neutral dye cell dyeing liquid, and serge blue for example, peacock green, Yihong etc., working concentration is 001-1.0%.Described physiological saline also can be pure water and the destainer that contains acidic materials such as low-concentration acetic acid.
A kind of preparation method of semi-quantitative sperm concentration rapid self-inspection kit comprises the steps:
Preparation cell dyeing agent solution: stain for cell is added phosphate buffer fully dissolve, filter rearmounted room temperature preservation;
The preparation standard colour code: the sperm sample with 5,000,000,1,000 ten thousand, 2,000 ten thousand, 3,000 ten thousand, 4,000 ten thousand, 6,000 ten thousand, 8,000 ten thousand, the 10000 ten thousand/ml concn of confirming through microscopic count is a master sample, add the coloring agent solution that has prepared, color according to experimental result, use modern printing technology and prepare the standard colour code, place on the shell around the test hole;
Filtering membrane and thieving paper are placed the substrate top successively, the test hole below.
Advantage of the present invention: the concentration that can detect sperm rapidly and accurately, thereby diagnose out the male sex whether can procreate, save a lot of times than traditional in the past diagnostic method, use simply, use and get final product, do not spend hospital admission in pharmacy's purchase, family, make the user not have shy sense, make the man and wife can know the potential fertility Issue of oneself in advance, use this kit to detect, also can be used for sperm bank sperm quality is screened effect after man's sterilization operation.
Description of drawings
The present invention will be further described in detail below in conjunction with accompanying drawing.
Fig. 1 is the structural representation of breadboard of the present invention.
Fig. 2 is the part-structure synoptic diagram of breadboard of the present invention.
1. substrates, 2. shells, 3. test holes, 4. filtering membranes, 5. thieving papers, 6. standard colour codes among the figure
Embodiment
As seen from the figure, breadboard of the present invention comprises substrate 1 and shell 2, and there is a test hole 3 in shell 2 central authorities, and filtering membrane 4, thieving paper 5 and standard colour code 6 are arranged on the substrate 1, and filtering membrane 4 and thieving paper 5 are positioned at substrate 1 top, test hole 3 belows; Thieving paper 5 is positioned at filtering membrane 4 belows; Standard colour code 6 is positioned on the shell 2 around the test hole 3.
Standard colors target color is the color that 2,000 ten thousand/ml concn sample of sperm adds the cell dyeing liquid test findings.
A kind of method for preparing the sperm concentration quick detection kit is an example with the serge blue stain for cell:
Preparation concentration is 0.1% methylene blue solution: precision takes by weighing 105 ℃ of biological stain serge blue 100mg that are dried to constant weight, puts in the 1000ml measuring bottle, adds phosphate buffer to scale, shakes up, and filters, and filters rearmounted room temperature preservation;
The preparation standard colour code: the sperm sample with 5,000,000,1,000 ten thousand, 2,000 ten thousand, 3,000 ten thousand, 4,000 ten thousand, 6,000 ten thousand, 8,000 ten thousand, the 10000 ten thousand/ml concn of confirming through microscopic count is a master sample, add the methylene blue solution that has prepared, color according to experimental result, prepare the standard colour code, use modern printing technology and prepare the standard colour code, place on the shell around the test hole;
Filtering membrane and thieving paper are placed the substrate top successively, the test hole below.
The using method of sperm concentration quick detection kit is as follows:
1, collects semen sample in the seminal fluid collecting box;
2, seminal fluid liquefaction allows seminal fluid liquefy in the seminal fluid collecting box, and the general time is 15 to 30 minutes, is determined at behind the seminal fluid collecting to finish in 12 hours;
3, application of sample is collected 1 seminal fluid with little plastic suction pipe and is added in the test hole, allow test hole inner filtration film fully soak 1 minute and allows thieving paper blot moisture content on the film;
4, add 2 physiological saline to test hole, allow test hole inner filtration film fully soak 1 minute and allow thieving paper blot moisture content on the film;
5, add 2 methylene blue solutions to test hole, allow test hole inner filtration film fully soak 1 minute and allow thieving paper blot moisture content on the film;
6, add 2 physiological saline to test hole, allow test hole inner filtration film fully soak 1 minute, begin to read the result after waiting thieving paper to blot moisture content on the film.To in 4 minutes, read the result, inaccurate above reading the possibility of result after 4 minutes;
7, the change color in viewing test hole, and with the color contrast that standard colors is put on, determine immediate color, sentence read result.
8, test for the second time:
Per twice test is at interval more than the week.
Claims (7)
1, a kind of semi-quantitative sperm concentration rapid self-inspection kit, it is characterized in that: comprise seminal fluid collecting box, cell dyeing liquid, physiological saline and breadboard, described seminal fluid collecting box collects box by plastics and the seminal fluid liquefier is formed, described breadboard comprises shell, substrate, shell and substrate are that rectangular PVC plastics are made, there is a test hole in shell central authorities, thieving paper, filtering membrane and standard colour code are arranged on the described substrate, thieving paper and filtering membrane are positioned at the substrate top, the test hole below of shell; Thieving paper is positioned at the filtering membrane below; The standard colors mark is around test hole on the shell, and standard colors target color is the color that 5,000,000,1,000 ten thousand, 2,000 ten thousand, 3,000 ten thousand, 4,000 ten thousand, 6,000 ten thousand, 8,000 ten thousand, 10,000 ten thousand/ml concn sample of sperm adds the cell dyeing liquid test findings.
2, a kind of semi-quantitative sperm concentration rapid self-inspection kit according to claim 1, it is characterized in that: described filtering membrane is the hydrophilic glass tunica fibrosa, pore diameter range 1-30 micron, size dimension are 10 millimeters * 10 millimeters; Described thieving paper is lignocellulose paper, and its size dimension is consistent with filtering membrane.
3, a kind of semi-quantitative sperm concentration rapid self-inspection kit according to claim 1, it is characterized in that: described seminal fluid liquefier can be enzyme preparation such as bromelain, pronase or chymotrypsin, non-enzyme preparation such as dithiothreitol (DTT), working concentration is 1-5mg/ml.
4, a kind of semi-quantitative sperm concentration rapid self-inspection kit according to claim 1 is characterized in that: described cell dyeing liquid can be acid, alkalescence and neutral dye cell dyeing liquid, serge blue for example, peacock green, Yihong etc., working concentration is 0.01-1.0%.
5, a kind of method for preparing each described semi-quantitative sperm concentration rapid self-inspection kit of claim 1-4, comprising following steps:
Preparation cell dyeing agent solution: stain for cell is added phosphate buffer fully dissolve, filter rearmounted room temperature preservation;
The preparation standard colour code: the sperm sample with 5,000,000,1,000 ten thousand, 2,000 ten thousand, 3,000 ten thousand, 4,000 ten thousand, 6,000 ten thousand, 8,000 ten thousand, the 10000 ten thousand/ml concn of confirming through microscopic count is a master sample, add the coloring agent solution that has prepared, color according to experimental result, use modern printing technology and prepare the standard colour code, place on the shell around the test hole;
Filtering membrane and thieving paper are placed the substrate top successively, the test hole below.
6, a kind of method of using each described semi-quantitative sperm concentration rapid self-inspection kit of claim 1-4: comprising following steps:
Collect semen sample in the seminal fluid collecting box;
Seminal fluid liquefaction allows seminal fluid liquefy in the seminal fluid collecting box, and the general time is 15 to 30 minutes, is determined at behind the seminal fluid collecting to finish in 12 hours;
Application of sample is collected 1 seminal fluid with little plastic suction pipe and is added in the test hole, allow test hole inner filtration film fully soak 1 minute and allows thieving paper blot moisture content on the film;
Add 2 physiological saline to test hole, allow test hole inner filtration film fully soak 1 minute and allow thieving paper blot moisture content on the film;
Add the cell coloring agent, add 2 stain for cell, allow test hole inner filtration film fully soak 1 minute and allow thieving paper blot moisture content on the film to test hole;
Add 2 physiological saline to test hole, allow test hole inner filtration film fully soak 1 minute, begin to read the result after waiting thieving paper to blot moisture content on the film, in 4 minutes, read the result, surpass that to read the possibility of result after 4 minutes inaccurate;
The change color in viewing test hole, and with the color contrast that standard colors is put on, determine immediate color, sentence read result.
Test for the second time:
Per twice test is at interval more than the week.
7, according to claim 1 or 6 described semi-quantitative sperm concentration rapid self-inspection kit and using method thereof, it is characterized in that: described physiological saline also can be pure water and the destainer that contains acidic materials such as low-concentration acetic acid.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200910081425A CN101644706A (en) | 2009-04-03 | 2009-04-03 | Semi-quantitative sperm concentration rapid self-test kit, prepration method and using method thereof |
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| CN200910081425A CN101644706A (en) | 2009-04-03 | 2009-04-03 | Semi-quantitative sperm concentration rapid self-test kit, prepration method and using method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012055077A1 (en) * | 2010-10-30 | 2012-05-03 | 湖南省天骑医学新技术有限公司 | Method and device for staining and slide preparation of cells or bacteria |
| CN103773672A (en) * | 2014-02-08 | 2014-05-07 | 浙江省医学科学院 | Method and device for detecting motility and chemotaxis of human sperms |
| CN103969193A (en) * | 2013-01-28 | 2014-08-06 | 郑兆珉 | sperm quality detection device |
| WO2016037536A1 (en) * | 2014-09-11 | 2016-03-17 | 天津起跑线生物信息技术有限公司 | Quantitative test method of semen liquefying capability |
| CN107179401A (en) * | 2017-06-26 | 2017-09-19 | 河北工业大学 | A kind of sperm quality rapid detection system and detection method based on micro-fluidic chip |
| CN117405903A (en) * | 2023-12-13 | 2024-01-16 | 阅尔基因技术（苏州）有限公司 | Kit for detecting male infertility gene and application method thereof |
-
2009
- 2009-04-03 CN CN200910081425A patent/CN101644706A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012055077A1 (en) * | 2010-10-30 | 2012-05-03 | 湖南省天骑医学新技术有限公司 | Method and device for staining and slide preparation of cells or bacteria |
| CN103969193A (en) * | 2013-01-28 | 2014-08-06 | 郑兆珉 | sperm quality detection device |
| CN103773672A (en) * | 2014-02-08 | 2014-05-07 | 浙江省医学科学院 | Method and device for detecting motility and chemotaxis of human sperms |
| CN103773672B (en) * | 2014-02-08 | 2015-02-25 | 浙江省医学科学院 | Method and device for detecting motility and chemotaxis of human sperms |
| WO2016037536A1 (en) * | 2014-09-11 | 2016-03-17 | 天津起跑线生物信息技术有限公司 | Quantitative test method of semen liquefying capability |
| US10247664B2 (en) | 2014-09-11 | 2019-04-02 | Ahead Biotech Co., Ltd. | Quantificational testing method for semen liquefaction ability |
| CN107179401A (en) * | 2017-06-26 | 2017-09-19 | 河北工业大学 | A kind of sperm quality rapid detection system and detection method based on micro-fluidic chip |
| CN117405903A (en) * | 2023-12-13 | 2024-01-16 | 阅尔基因技术（苏州）有限公司 | Kit for detecting male infertility gene and application method thereof |
| CN117405903B (en) * | 2023-12-13 | 2024-02-20 | 阅尔基因技术（苏州）有限公司 | Kit for detecting male infertility gene and application method thereof |
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Application publication date: 20100210 |