CN117405903B - Kit for detecting male infertility gene and application method thereof - Google Patents
Kit for detecting male infertility gene and application method thereof Download PDFInfo
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- CN117405903B CN117405903B CN202311703452.1A CN202311703452A CN117405903B CN 117405903 B CN117405903 B CN 117405903B CN 202311703452 A CN202311703452 A CN 202311703452A CN 117405903 B CN117405903 B CN 117405903B
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- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 21
- 208000007466 Male Infertility Diseases 0.000 title claims abstract description 16
- 238000012360 testing method Methods 0.000 claims abstract description 170
- 238000001514 detection method Methods 0.000 claims abstract description 106
- 238000001179 sorption measurement Methods 0.000 claims description 120
- 239000007788 liquid Substances 0.000 claims description 35
- 238000010030 laminating Methods 0.000 claims description 25
- 238000005192 partition Methods 0.000 claims description 12
- 210000001503 joint Anatomy 0.000 claims description 10
- 238000001125 extrusion Methods 0.000 claims description 8
- 238000003032 molecular docking Methods 0.000 claims description 6
- 230000000903 blocking effect Effects 0.000 claims description 4
- 238000003780 insertion Methods 0.000 claims description 3
- 230000037431 insertion Effects 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims 5
- 230000000712 assembly Effects 0.000 claims 2
- 238000000429 assembly Methods 0.000 claims 2
- 208000000509 infertility Diseases 0.000 abstract description 10
- 230000036512 infertility Effects 0.000 abstract description 10
- 231100000535 infertility Toxicity 0.000 abstract description 6
- 102000004169 proteins and genes Human genes 0.000 abstract description 5
- 102100032131 Lymphocyte antigen 6E Human genes 0.000 abstract description 3
- 101710177157 NHP2-like protein 1 Proteins 0.000 abstract description 3
- 101000661610 Sus scrofa Spermatid nuclear transition protein 4 Proteins 0.000 abstract description 3
- 108010037277 thymic shared antigen-1 Proteins 0.000 abstract description 3
- 238000012864 cross contamination Methods 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 description 27
- 238000004140 cleaning Methods 0.000 description 12
- 241000894006 Bacteria Species 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 208000021267 infertility disease Diseases 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000011358 absorbing material Substances 0.000 description 3
- 238000007664 blowing Methods 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 238000005086 pumping Methods 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 239000010893 paper waste Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000000469 anti-sperm effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B5/00—Cleaning by methods involving the use of air flow or gas flow
- B08B5/02—Cleaning by the force of jets, e.g. blowing-out cavities
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/02—Cleaning by the force of jets or sprays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B5/00—Cleaning by methods involving the use of air flow or gas flow
- B08B5/04—Cleaning by suction, with or without auxiliary action
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/36—Gynecology or obstetrics
- G01N2800/367—Infertility, e.g. sperm disorder, ovulatory dysfunction
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kit for detecting male infertility genes, which comprises a box body, wherein a through hole is formed in one side, close to the bottom end, of the box body, a hanging plate is fixedly connected to the inner side, located above the through hole, of the box body, a first telescopic rod is fixedly connected to one side, facing the through hole, of the hanging plate, an attaching plate is fixedly connected to the output end of the first telescopic rod, the attaching plate is attached to the inner wall of the through hole, and a detection table is placed at the bottom of the box body. The application method of the kit for detecting male infertility genes is also disclosed, and the kit can simultaneously detect multiple people and three recombinant human sperm proteins, namely human sperm proteins TSA-1, FA-1 and STP-4, so as to indirectly determine whether the infertility genes exist; the kit is pushed out from the kit body through the pushing-out component, so that the manual replacement of the test paper and the pollution of the internal space during the replacement are avoided, and the accuracy of the detection result is improved; the kit can be automatically cleaned and repeatedly used, so that cross contamination is avoided, and the kit is environment-friendly and reliable.
Description
Technical Field
The invention relates to the technical field of infertility detection, in particular to a kit for detecting male infertility genes and a use method thereof.
Background
More and more married couples of child bearing age in today's society are plagued by infertility problems, and about 10-15% of the couples of child bearing age are statistically not viable for a variety of reasons. Wherein the immune infertility caused by anti-sperm antibodies induced by sperm antigens in vivo account for a significant proportion.
The patent with publication number of CN204359793U discloses a sterility test kit structure, which comprises a box, a detachable enzyme-linked plate arranged in the box, a first positive control liquid bottle, a second positive control liquid bottle, a first test liquid bottle, a second test liquid bottle, a test diluent bottle, a washing stock solution bottle, a first substrate solution bottle, a second substrate solution bottle and a stop liquid bottle which are arranged in the box, wherein three recombinant human sperm eggs are coated on micropores of the enzyme-linked plate, and the three recombinant human sperm proteins, namely human sperm protein TSA-1, FA-1 and STP-4, can be used for determining whether antibodies of three sperm proteins related to immune sterility exist in a patient body after corresponding reagents are added in the sterility test kit structure, and provides a new, low-cost and definite rapid detection means for screening of immune sterility patients. The antibodies of the three sperm proteins related to the immune infertility are edited into corresponding gene sequences, so that similar detection means can indirectly judge the existence of the infertility genes from macroscopic view.
In addition, in the using process of the similar sterile gene detection kit, after the detection liquid is detected by the test paper, the test paper needs to be replaced by a worker, and the detected test paper is taken out. In the process of replacing test paper by staff, the test paper is easy to be polluted by the external environment, and the detection accuracy is affected; and most of similar kits are disposable, which is easy to cause resource waste.
Disclosure of Invention
The invention discloses a kit for detecting male infertility genes, and aims to solve the problems in the background technology.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the kit for detecting male infertility genes comprises a box body, wherein a through hole is formed in one side, close to the bottom end, of the box body, a laminating plate is connected in the through hole in a sealing manner, a hanging plate is fixedly connected to the inner side, located above the through hole, of the box body, a first telescopic rod is fixedly connected to one side, facing to the through hole, of the hanging plate, the output end of the first telescopic rod is fixedly connected with the laminating plate, the laminating plate is attached to the inner wall of the through hole, a detection table is placed at the bottom of the box body, a blocking rod is fixedly connected to the box body, a placing groove is formed in the top of the detection table in an equidistant manner, a replacement component is arranged in each placing groove, push-out components are arranged at the two ends of each replacement component, each replacement component comprises an arc-shaped placing plate, a jacking telescopic rod is fixedly connected to the inner wall of the bottom of each placing groove, the arc is placed board fixedly connected with in jack-up telescopic link's output, and the standing groove is located the both sides inner wall that the arc placed board top all through bearing connection there being the connecting axle, and the equal fixedly connected with in outside of two connecting axles is spacing contact roller, detect the equal fixedly connected with second telescopic link in top that the platform is located two spacing contact roller tops, the equal fixedly connected with adsorption plate of output of two second telescopic links, the bottom equidistance of adsorption plate leaves has the suction opening, the equal fixedly connected with side stock in both sides of detecting the platform, the equidistant fixedly connected with adsorption pump two in top of side stock, the adsorption end of adsorption pump two passes through the inside of pipe connection in corresponding adsorption plate, and two adsorption plates are located the top of two spacing contact rollers.
Through being provided with change subassembly and ejecting component, when detecting liquid detects, adjust jack-up telescopic link drive the arc and place the test paper of board top and shift up, make the slow follow two spacing contact rollers of test paper of top remove, then adjust the top contact of second telescopic link drive adsorption plate and test paper of top, start adsorption pump two, adsorption pump two realizes the quick absorption of test paper, the second telescopic link drives the adsorption plate and resets, then the test paper is located the standing groove and is close to inside the top, after test paper uses, release it from the box body through ejecting component, thereby avoid the repeated change of test paper to lead to the box body inside to be close to the space of below contaminated, avoid bacterium in the air to be in a large number attached to test paper department, thereby improve the accuracy of testing result.
In a preferred scheme, the ejecting subassembly includes the ejecting frame, the inside cavity that is equipped with of ejecting frame, and laminating board and detection platform all open in the outside that is close to the standing groove has the mounting hole, ejecting frame fixed connection is in the inside of two mounting holes, it has the removal hole to detect one side that the platform kept away from ejecting frame, mounting hole, removal hole and standing groove all are in the state of intercommunication, the ejecting frame is located the top fixedly connected with ejector pin of box body outside, the ejector pin is facing the outside fixedly connected with third telescopic link of below, the box body is located the top of hydraulic rod below and opens there is the shutoff hole, the inside grafting of shutoff hole has the shutoff board, the output fixedly connected with of third telescopic link is in the top of shutoff board.
In a preferred scheme, one side of the detection table, which is close to the moving hole, is fixedly connected with a mounting frame, one side of the mounting frame, which faces the detection table, is fixedly connected with a fourth telescopic rod, the output end of the fourth telescopic rod is fixedly connected with a pushing plate, the top of the pushing plate is fixedly connected with a contact piece, and the contact piece is made of a water absorbing material; the outside fixedly connected with absorption pipe that the mounting bracket was kept away from to the impeller, and the outside of absorption pipe face upwards is opened there is the absorption hole, and the outside fixedly connected with pump frame of absorption pipe, the top fixedly connected with absorption pump of pump frame one, and the absorption end of absorption pump one passes through the inside that the pipe is connected in absorption pipe, and absorption pipe is located the below of impeller.
Through being provided with the release subassembly, detection test paper removes to the inside that the standing groove is close to the top, adjust fourth telescopic link and drive the flitch and remove, make the absorption pipe remove to the below of the detection test paper of keeping away from the mounting bracket, each contact piece contacts with the bottom of detection test paper, the contact piece plays the effect that detection test paper bottom supported with the absorption pipe, after detection test paper used up, start absorption pump one, absorption pump two is closed, absorption hole on the absorption pipe adsorbs detection test paper, fourth telescopic link is adjusted once more, thereby remove detection test paper to the outside of box body through the release frame, the staff collects it, it is convenient high-efficient.
In a preferred scheme, one side that the box body is located the laminating board below fixedly connected with two supporting guide rail, and the outside fixedly connected with of laminating board two support slider, two support slider sliding connection are in the inside of adjacent supporting guide rail, and the top of box body has the lid through hinge connection, and the top fixedly connected with of lid carries the pull handle.
In a preferred scheme, the box body is located the inner wall fixedly connected with division board of detecting bench top, and division board's top equidistance leaves there is the jack, and the inner wall of every jack all is pegged graft there is the test tube, and the outside fixedly connected with outside spacing ring board of test tube, outside spacing ring board contact with division board's top, and open the bottom of test tube has the flowing back hole, and the inside fixedly connected with flowing back pipe of flowing back hole, the outside of flowing back pipe has the metering valve through flange joint.
In a preferred scheme, the bottom fixedly connected with dead lever of test tube, and dead lever one side towards the fluid-discharge tube fixedly connected with fifth telescopic link, the output fixedly connected with drip plate of fifth telescopic link, the bottom equidistance of drip plate leaves there is the drip hole, and open at the top of drip plate has the connecting hole, and the inside fixedly connected with butt joint hose of connecting hole, the other end of butt joint hose peg graft in the inside of fluid-discharge tube.
Through being provided with drip board and dropping hole, when detecting the liquid and discharging, the fifth telescopic link drives the drip board and removes to evenly drip the detection liquid to the test paper through the dropping hole, improve the homogeneity of detecting the liquid drip, and then improve the accuracy of testing result.
In a preferred scheme, the outside fixedly connected with outer loop frame that the division board is located the jack below, and the inboard equidistance fixedly connected with extrusion spring pole of outer loop frame, the inboard equidistance of outer loop frame is through hinged joint having laminating grip block, and laminating grip block contacts with the outside of test tube, and the other end fixedly connected with of extrusion spring pole is in the outside of laminating grip block.
In a preferred scheme, the box body is located the same communicating pipe of both sides inner wall fixedly connected with of division board top, and the outside equidistance of communicating pipe leaves there is the minute hole, the equal fixedly connected with in the inner wall in every minute hole is divided the pipe, the division board is located the top fixedly connected with connecting block in every test tube outside, the outside fixedly connected with exhaust tube of connecting block, the annular of the outside of exhaust tube face below is opened there is the gas vent, be in the state of intercommunication with the exhaust tube in the exhaust tube, one side fixedly connected with fixed plate of box body, the top fixedly connected with aspiration pump of fixed plate, the exhaust end fixedly connected with exhaust tube of aspiration pump, the other end grafting of exhaust tube is in the inside of communicating pipe.
Through setting up laminating grip block, extrusion spring pole, exhaust tube and aspiration pump, when carrying out the laying of test tube, with it impress between each laminating grip block, the reaction force of extrusion spring pole drives the laminating grip block and extrudes the centre gripping to the test tube, improves the convenience of test tube centre gripping, when the test tube takes out the back, the lid is in the state of opening, starts the aspiration pump, and the aspiration pump will probably get into the air extraction of division board below to the box body outside, further prevents that bacterium in the air from being located the air of division board below to the box body and causing the pollution.
In a preferred scheme, the lid is located the top of every test tube top and all opens and have the hole of agreeing with, and the equal fixedly connected with arbor board in both sides of box body, the same axis of rotation is connected with through the bearing to the opposite side of two arbor boards, the outside equidistance fixedly connected with of axis of rotation rotates the cover, the equal fixedly connected with of the outside of every rotates the cover agrees with the board, it laminates with the hole of agreeing with, it has the limiting hole to agree with the top of board to open, the inner wall in limiting hole peg graft has the butt joint feed liquor mouth, the butt joint feed liquor mouth is located the top of test tube, the one end fixedly connected with of axis of rotation rotates the handle, the box body is located the outside fixedly connected with cardboard of a plurality of rotation cover below.
The invention also provides a use method of the kit for detecting male infertility genes, which comprises the following steps:
step one: connecting the sample with detection with the docking liquid inlet nozzle, and adding the sample into a test tube; adjusting the jacking telescopic rod to drive the detection test paper above the arc-shaped placing plate to move upwards, enabling the uppermost detection test paper to move between the two limiting contact rollers, adjusting the second telescopic rod to drive the adsorption plate to contact with the top of the uppermost detection test paper, starting the adsorption pump II, adsorbing the detection test paper by the adsorption pump II, enabling the adsorption plate to be driven by the second telescopic rod to reset, and enabling the test paper to be located in the placing groove close to the top;
step two: the metering valve is opened, the fifth telescopic rod is controlled to drive the dropping plate to move, and the detection liquid is uniformly dropped onto the detection test paper through the dropping hole;
step three: after the detection of test paper is completed, the first adsorption pump is started, the second adsorption pump is closed, the adsorption hole on the adsorption round tube adsorbs the test paper, the fourth telescopic rod stretches, the test paper is moved to the outside of the box body through the push-out frame, and collection of the test paper is completed.
The invention has at least the following technical effects:
1. when the detection liquid is detected, the jacking telescopic rod is regulated to drive the detection test paper above the arc-shaped placing plate to move upwards, so that the uppermost detection test paper slowly moves from the two limiting contact rollers, then the second telescopic rod is regulated to drive the adsorption plate to contact with the top of the uppermost detection test paper, the adsorption pump II is started, the adsorption pump II realizes the rapid adsorption of the detection test paper, the second telescopic rod drives the adsorption plate to reset, the test paper is positioned in the placing groove near the top, after the detection test paper is used, the test paper is pushed out of the box body through the push-out assembly, the space near the lower part in the box body is prevented from being polluted due to repeated replacement of the detection test paper, and a large amount of bacteria in air are prevented from being attached to the detection test paper, so that the technical effect of accuracy of detection results is improved.
2. According to the invention, the fourth telescopic rod is controlled to extend to the fourth length, so that the adsorption round tube moves to be close to the position right below the adsorption plate, then the second telescopic rod is controlled to drive the adsorption plate to move downwards, the adsorption hole is communicated with the adsorption hole, the second adsorption pump is controlled to exhaust air, and the first adsorption pump is controlled to rotate reversely and blow air, so that the first adsorption pump and the second adsorption pump act on the blocked adsorption hole together, and dredging of the blocked adsorption hole is completed under the cooperation of air suction and blowing, abnormal detection caused by influence of the adsorption hole on the detection test paper is prevented, and smooth detection work is ensured.
3. When the cleaning device is used for cleaning, firstly, cleaning liquid is added into the test tube, the fourth telescopic rod is controlled to extend to a fifth length, so that the fourth telescopic rod drives the pushing plate to move to the position right below the dripping plate, after the metering valve is controlled to be opened, the cleaning liquid in the test tube can be sprayed onto the pushing plate, the contact piece and the adsorption round tube above the pushing plate through the dripping hole below the dripping plate, and the cleaning device is used for cleaning the pushing plate; and the second telescopic rod is controlled to stretch so as to drive the adsorption plates and the contact pieces to be in extrusion contact, the fourth telescopic rod is further controlled to stretch repeatedly to a preset length on the basis of keeping the fourth telescopic rod to stretch to a fifth length, samples adhered to the two adsorption plates can be erased, the contact pieces can be cleaned, the influence of residual samples on subsequent detection is prevented, and the accuracy of detection results is further guaranteed.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the kit of the present invention.
FIG. 2 is a schematic diagram of the internal structure of the kit of the present invention.
FIG. 3 is a schematic diagram showing the combined structure of the test table and the test tube of the kit of the present invention.
FIG. 4 is a schematic view showing the combined structure of the replacement assembly and the pushing assembly of the kit of the present invention.
FIG. 5 is a cross-sectional view showing the structure of a test bench of the kit of the present invention.
FIG. 6 is a schematic diagram of a replacement assembly for a kit of the present invention.
FIG. 7 is a schematic view showing the combined structure of a test tube and a bonding clamping plate of the kit of the invention.
FIG. 8 is a schematic view showing the combined structure of the drip plate and the attaching and clamping plate of the kit of the present invention.
FIG. 9 is a schematic view of the combination structure of the engagement plate and the docking nozzle of the kit of the present invention.
In the figure: 1. a case body; 2. a support slider; 3. a push-out assembly; 301. a push-out frame; 302. a plugging plate; 303. a push rod; 304. a third telescopic rod; 305. a fourth telescopic rod; 306. a mounting frame; 307. a pushing plate; 308. a contact piece; 309. adsorbing the round tube; 310. adsorption holes; 311. a first adsorption pump; 312. a pump frame; 4. a support rail; 5. bonding plates; 6. a box cover; 7. a lifting handle; 8. a wedge plate; 9. docking a liquid inlet nozzle; 10. a clamping plate; 11. rotating the handle; 12. a detection table; 13. a blocking rod; 14. a communicating pipe; 15. a rotating sleeve; 16. a rotating shaft; 17. a test tube; 18. an exhaust pipe; 19. a connecting block; 20. a partition plate; 21. a first telescopic rod; 22. a hanger plate; 23. drawing out a pipe; 24. an air extracting pump; 25. a fixing plate; 26. a placement groove; 27. a branch pipe; 28. a side long rod; 29. replacing the assembly; 2901. an arc-shaped placing plate; 2902. limiting the contact roller; 2903. an adsorption plate; 2904. a connecting shaft; 2905. a second telescopic rod; 2906. adsorption pump II; 2907. a suction hole; 2908. jacking up the telescopic rod; 30. an air suction hole; 31. a drip plate; 32. a drip hole; 33. a fifth telescopic rod; 34. a fixed rod; 35. an outer limit ring plate; 36. a liquid discharge pipe; 37. docking the hose; 38. a metering valve; 39. extruding a spring rod; 40. an outer ring frame; 41. attaching a clamping plate; 42. and a shaft plate.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments.
First embodiment
Referring to fig. 1 to 9, a kit for detecting male infertility genes comprises a box body 1, wherein a through hole is formed in one side, close to the bottom end, of the box body 1, an attaching plate 5 is connected in the through hole in a sealing manner, a hanging plate 22 is fixedly connected to the inner side, located above the through hole, of the box body 1, a first telescopic rod 21 is fixedly connected to one side, facing the through hole, of the hanging plate 22, and the output end of the first telescopic rod 21 is fixedly connected with the attaching plate 5. A detection table 12 is arranged at the bottom of the box body 1, and a blocking rod 13 is connected to the box body 1 close to the detection table 12. The bonding board 5 is connected to the inspection table 12 through the push-out unit 3. The setting of adhesion plate 5 can cooperate with first telescopic link 21, pushes adhesion plate 5 outward to box body 1 through first telescopic link 21, and then outside pushing out box body 1 to detection platform 12 through pushing out subassembly 3 to the convenience is packed the test paper on the detection platform 12 or is maintained other parts.
The top equidistance of detecting table 12 leaves there is standing groove 26, and the inside of every standing groove 26 all is equipped with and changes subassembly 29, and the standing groove 26 is located every and changes subassembly 29 both ends and be equipped with ejecting assembly 3. Wherein, change subassembly 29 includes that the arc is placed board 2901, and the bottom inner wall fixedly connected with jack-up telescopic link 2908 of standing groove 26, and board 2901 fixed connection is placed in jack-up telescopic link 2908's output in the arc, and the standing groove 26 is located the both sides inner wall of board 2901 top and all is connected with connecting axle 2904 through the bearing, and the equal fixedly connected with spacing contact roller 2902 in the outside of two connecting axles 2904. The top that detection platform 12 is located two spacing contact roller 2902 top all fixedly connected with second telescopic link 2905, and the equal fixedly connected with of output of two second telescopic links 2905 adsorbs board 2903, and the bottom equidistance of adsorbing board 2903 leaves has suction hole 2907. The two sides of the detection table 12 are fixedly connected with side long rods 28, the tops of the side long rods 28 are fixedly connected with adsorption pumps II 2906 at equal intervals, the adsorption ends of the adsorption pumps II 2906 are connected to the interiors of corresponding adsorption plates 2903 through pipelines, and the two adsorption plates 2903 are located above the two limit contact rollers 2902.
Through the setting of above-mentioned structure, when detecting the sample, adjust jack-up telescopic link 2908 and drive the arc and place board 2901 and the test paper of its top and shift up for the test paper of top is slow from the upward movement between two spacing contact rollers 2902, then adjusts the top contact of second telescopic link 2905 drive adsorption plate 2903 and the test paper of top. In this process, after the test paper is placed on the arc placing plate 2901, the arc placing plate 2901 is matched with the two limiting contact rollers, so that the test paper is bent and stretched at two ends, and accumulation caused by folds is avoided, and infiltration and diffusion of a sample in the test paper are facilitated. After the test paper is sent to a certain height, the second adsorption pump 2906 is started, the second adsorption pump 2906 realizes quick adsorption of the test paper, the second telescopic rod 2905 drives the adsorption plate 2903 to reset, the test paper is located inside the placing groove 26 close to the top end, and preparation is made for sample addition at the moment. After the test strip is added to the sample and reacted for a prescribed time, it is pushed out from the cartridge 1 by the push-out assembly 3 and the test strip after detection is collected. Therefore, when the same sample is prevented from being repeatedly detected, the detection test paper is easy to pollute when being manually replaced, and the detection test paper is prevented from being replaced to pollute the space, close to the lower part, inside the box body 1, of the detection test paper, bacteria in the air are prevented from being attached to the detection test paper, and therefore accuracy of detection results is improved.
Referring to fig. 1, 3, 4, 5 and 6, in a preferred embodiment, the ejector assembly 3 includes an ejector frame 301, with a cavity provided within the ejector frame 301. And the outside that laminating board 5 and detection platform 12 are close to standing groove 26 all opens there is the mounting hole, and ejecting frame 301 fixed connection is in the inside of two mounting holes, and detection platform 12 is kept away from one side of ejecting frame 301 and is opened there is the removal hole (specifically the one side that detection platform 12 kept away from laminating board 5), and mounting hole, removal hole and standing groove 26 all are in the state of intercommunication. The top that ejecting frame 301 is located the box body 1 outside fixedly connected with ejector pin 303, and ejector pin 303 faces the outside fixedly connected with third telescopic link 304 of below, and ejecting frame 301 is located the top of third telescopic link 304 below and opens has the shutoff hole, and the shutoff hole runs through ejecting frame 301 and is linked together with the cavity in the ejecting frame 301. The inside grafting of shutoff hole has shutoff board 302, and the output fixed connection of third telescopic link 304 is in the top of shutoff board 302. In the normal detection process of the device, the third telescopic rod 304 is controlled to drive the plugging plate 302 to be inserted into the plugging hole and seal the cavity of the push-out frame 301, so that the external air is prevented from entering the content of the box body 1, polluting a sample and affecting the accuracy of sample detection. After the detection is completed, the third telescopic rod 304 is controlled to drive the plugging plate 302 to be opened, so that preparation is made for pushing out the detection test paper after the detection is completed from the cavity of the push-out frame 301.
One side of the detection table 12 close to the moving hole is fixedly connected with a mounting frame 306, one side of the mounting frame 306 facing the detection table 12 is fixedly connected with a fourth telescopic rod 305, the output end of the fourth telescopic rod 305 is fixedly connected with a pushing plate 307, the top of the pushing plate 307 is fixedly connected with a contact piece 308, and the contact piece 308 is made of water-absorbing materials such as sponge, cotton cloth and the like. The outside fixedly connected with absorption pipe 309 that promotes board 307 kept away from mounting bracket 306, absorption pipe 309 upwards opens in the outside that faces upwards has absorption hole 310, and the outside fixedly connected with pump frame 312 of absorption pipe 309, and the top fixedly connected with absorption pump 311 of pump frame 312, the absorption end of absorption pump 311 passes through the inside of pipeline connection in absorption pipe 309, and absorption pipe 309 is located the below of absorption board 2903.
It should be noted that, after the arc-shaped placing plate 2901 drives the test paper to move to the inside of the placing groove 26 near the upper side, and the two adsorption pumps two 2906 are started, after the adsorption pumps two 2906 realize rapid adsorption of the test paper through the adsorption holes 2907 on the adsorption plate 2903, the fourth telescopic rod 305 is adjusted to extend to the first length so as to drive the pushing plate 307 to move, so that the adsorption round tube 309 and the contact piece 308 move to the lower side of the test paper, the contact piece 308 and the adsorption round tube 309 contact with the bottom of the test paper, the contact piece 308 and the bottom of the test paper support effect on one hand, and the contact piece 308 can adsorb redundant samples to prevent the redundant samples from being spilled on the unused test paper below. Furthermore, the adsorbing tube 309 under the test strip can be prepared for adsorbing and driving the test strip after detection. After the test paper is used, the first adsorption pump 311 is started, the second adsorption pump 2906 is closed, the adsorption hole 310 on the adsorption round tube 309 adsorbs the test paper, the fourth telescopic rod 305 is adjusted to be extended to the second length, and therefore the test paper is pushed out of the box body 1 through the pushing plate 307 and the adsorption round tube 309 penetrating through the cavity of the push-out frame 301 (at this time, the third telescopic rod 304 drives the plugging plate 302 to be pulled out of the plugging hole, so that the cavity of the push-out frame 301 is in an open state), the test paper is conveniently collected by workers, samples are automatically added in the whole process, the test paper is pushed out, the interference of external factors is reduced, and the detection accuracy is improved.
Referring to fig. 1 and 2, in a preferred embodiment, two support rails 4 are fixedly connected to one side of the case 1 below the bonding plate 5, and two support sliders 2 are fixedly connected to the outer side of the bonding plate 5, and the two support sliders 2 are slidably connected to the inside of the adjacent support rails 4. The support rail 4 facilitates the insertion of the test strips or maintenance of the adhesive plate 5, the test table 12 and the related components out of the cartridge 1. The top of box body 1 has lid 6 through hinge connection, and lid 6 is connected in sealed with box body 1, reduces the entering of outside air, and then prevents to influence the accuracy of testing result. The top of the box cover 6 is fixedly connected with a lifting handle 7, so that the box cover 6 can be conveniently opened.
Referring to fig. 1, 2, 7 and 8, in a preferred embodiment, a partition plate 20 is fixedly connected to the inner wall of the box 1 above the detection table 12, and jacks are equidistantly arranged at the top of the partition plate 20, and test tubes 17 are inserted into the inner wall of each jack. The test tubes 17 are provided with a plurality of test tubes, so that the detection of multiple people can be realized simultaneously, and three recombinant human sperm proteins, namely human sperm proteins TSA-1, FA-1 and STP-4, can be detected simultaneously, so that the detection efficiency is improved, and whether the sterile gene exists or not is determined according to the detection result.
The outside of the test tube 17 is fixedly connected with an outside stop collar plate 35, and the outside stop collar plate 35 is contacted with the top of the partition plate 20. The bottom of test tube 17 is opened there is the fluid-discharge opening, the inside fixedly connected with fluid-discharge tube 36 of fluid-discharge opening, the outside of fluid-discharge tube 36 is through flange joint there being metering valve 38, the bottom fixedly connected with dead lever 34 of test tube 17, and the dead lever 34 is towards one side fixedly connected with fifth telescopic link 33 of fluid-discharge tube 36, the output fixedly connected with drip plate 31 of fifth telescopic link 33, drip plate 31's bottom equidistance is left there is drip hole 32, the top of drip plate 31 is opened there is the connecting hole, the inside fixedly connected with butt joint hose 37 of connecting hole, the other end of butt joint hose 37 is pegged graft in the inside of fluid-discharge tube 36.
Specifically, when the detection liquid is discharged, the fifth telescopic rod 33 drives the dropping plate 31 to move, so that the detection liquid is uniformly dropped onto the detection test paper through the dropping hole, the dropping uniformity of the detection liquid is improved, and the accuracy of the detection result is further improved.
Referring to fig. 1, 7 and 8, in a preferred embodiment, the outer side of the partition plate 20 below the insertion hole is fixedly connected with an outer ring frame 40, the inner side of the outer ring frame 40 is fixedly connected with a compression spring rod 39 at equal distance, the inner side of the outer ring frame 40 is fixedly connected with a fitting clamping plate 41 through a hinge, the fitting clamping plate 41 is in contact with the outer side of the test tube 17, and the other end of the compression spring rod 39 is fixedly connected with the outer side of the fitting clamping plate 41, so that the stability of fixing the test tube 17 is improved.
Referring to fig. 1, 2 and 3, in a preferred embodiment, the inner walls of two sides of the box body 1 above the partition plate 20 are fixedly connected with the same communicating pipe 14, the outer sides of the communicating pipes 14 are equidistantly separated by a split hole, the inner wall of each split hole is fixedly connected with a split pipe 27, the top of the partition plate 20 outside each test tube 17 is fixedly connected with a connecting block 19, the outer side of the connecting block 19 is fixedly connected with an exhaust pipe 18, the outer side of the exhaust pipe 18 facing downwards is annularly provided with an exhaust hole 30, and the split pipes 27 are in a communicating state with the exhaust pipe 18. One side of the box body 1 is fixedly connected with a fixing plate 25, the top of the fixing plate 25 is fixedly connected with an air pump 24, the air pumping end of the air pump 24 is fixedly connected with an air pumping pipe 23, and the other end of the air pumping pipe 23 is inserted into the communicating pipe 14.
In specific application scenario, when carrying out the laying of test tube 17, with it impress between each laminating grip block 41, the reaction force of extrusion spring bar 39 drives laminating grip block 41 and extrudees the centre gripping to test tube 17, improves the convenience of test tube 17 centre gripping, and after test tube 17 took out and changed, start aspiration pump 24, aspiration pump 24 will probably get into the air extraction of division board 20 below to the box body 1 outside, further prevent that bacterium or impurity etc. in the air that the sky got into from causing the pollution to the air of division board 20 below in the box body 1, influence the accuracy of testing result.
Referring to fig. 1, 2 and 9, in a preferred embodiment, the top of each test tube 17 above the box cover 6 is provided with a fitting hole, two sides of the box body 1 are fixedly connected with shaft plates 42, opposite sides of the two shaft plates 42 are connected with the same rotating shaft 16 through bearings, the outer sides of the rotating shafts 16 are fixedly connected with rotating sleeves 15 at equal intervals, the outer sides of each rotating sleeve 15 are fixedly connected with a fitting plate 8, the fitting plates 8 are attached to the fitting holes, the top of each fitting plate 8 is provided with a limiting hole, the inner wall of each limiting hole is inserted with a butt joint liquid inlet nozzle 9, the butt joint liquid inlet nozzle 9 is located above each test tube 17, one end of each rotating shaft 16 is fixedly connected with a rotating handle 11, and the outer sides of the box body 1 below the plurality of rotating sleeves 15 are fixedly connected with clamping plates 10, so that the test tubes 17 can be replaced or added with samples conveniently.
Second embodiment
In the detection process, the adsorption plates 2903 on both sides adsorb both ends of the detection test paper through the adsorption holes 2907, and are conveyed to the lower part of the drip plate 31 through the second telescopic rod 2905, and the drip plate 31 drops the sample onto the detection test paper through the drip holes 32. In the above detection process, the adsorption plate 2903 adsorbs two ends of the test paper through the adsorption hole 2907, so that normal sample detection can be ensured. And because the characteristic of test paper itself, after long-time work and many times absorption test paper, the gradual accumulation of tiny wastepaper on the test paper is in inhaling hole 2907, because the power that adsorption pump two 2906 actually selected is less, often is difficult to inhale away accumulated wastepaper, consequently causes inhaling hole 2907 to block easily. And once the suction hole 2907 is blocked, normal conveying of the test paper can be affected, so that normal detection cannot be performed. The suction holes 2907 need to be cleaned periodically to prevent the normal inspection operation from being affected.
Specifically, when the fourth telescopic rod 305 is not extended in the initial state, the fourth telescopic rod 305 is controlled to extend to the third length, the fourth telescopic rod 305 drives the adsorbing circular tube 309 to move to the position right below the adsorbing plate 2903 close to the pushing plate 307, at this time, the adsorbing holes 310 on the adsorbing circular tube 309 correspond to the adsorbing holes 2907 on the adsorbing plate 2903, then the second telescopic rod 2905 is controlled to drive the adsorbing plate 2903 to move downwards, so that the adsorbing holes 310 are communicated with the adsorbing holes 2907, at this time, the adsorbing pump two 2906 is controlled to suck air, and at the same time, the adsorbing pump one 311 is controlled to reversely rotate and blow air, so that the adsorbing circular tube 309 and the adsorbing circular tube are jointly acted on the blocked adsorbing hole 2907, and dredging of the blocked adsorbing hole 2907 is completed under the cooperation of air suction and blowing.
Similarly, the fourth telescopic rod 305 is controlled to extend to a fourth length, the fourth telescopic rod 305 drives the adsorbing circular tube 309 to move to be right below the adsorbing plate 2903 close to the side of the plugging plate 302, at the moment, the adsorbing holes 310 on the adsorbing circular tube 309 correspond to the adsorbing holes 2907 on the adsorbing plate 2903, then the second telescopic rod 2905 is controlled to drive the adsorbing plate 2903 to move downwards, so that the adsorbing holes 310 are communicated with the adsorbing holes 2907, at the moment, the second adsorbing pump 2906 is controlled to suck air, and meanwhile, the first adsorbing pump 311 is controlled to reversely rotate and blow air, so that the first adsorbing pump and the second adsorbing pump act on the blocked adsorbing hole 2907 together, and dredging of the blocked adsorbing hole 2907 is completed under the cooperation of air suction and blowing.
Through the operation, the suction hole 2907 working for a long time can be maintained, the blockage of the suction hole 2907 is dredged in time, the phenomenon that the suction hole 2907 cannot normally detect due to the fact that the suction hole 2907 adsorbs detection test paper is prevented from being influenced is avoided, and the smooth operation of detection work is guaranteed.
Third embodiment
In the above embodiment, the related parts of the replacement assembly 29 and the related parts of the push-out assembly 3, which are matched with the placement groove 26, are key parts for ensuring automatic push-out, collection and addition of test paper, so that external interference factors caused by manual operation of operators can be avoided to the greatest extent, and the accuracy of the inspection result is affected. In addition, the device is designed into a structure which can be repeatedly used by cleaning in order to avoid the defect that the disposable kit easily causes resource waste. In order to avoid the risk of cross-contamination between different samples during repeated use, it is therefore necessary to clean the above-mentioned relative parts of the replacement assembly 29, which are arranged in cooperation with the placement groove 26, and the relative parts of the ejection assembly 3 after use.
Specifically, when the above components are cleaned, cleaning solution is added into the test tube 17, the fourth telescopic rod 305 is controlled to extend to a fifth length, so that the fourth telescopic rod 305 drives the pushing plate 307 to move to the position right below the drip plate 31, and after the metering valve 38 is controlled to be opened, the cleaning solution in the test tube 17 can be sprayed onto the pushing plate 307, the contact piece 308 and the adsorption round tube 309 above the pushing plate 307 through the drip hole 32 below the drip plate 31, so as to clean the components. The contact piece 308 is made of water-absorbing material such as sponge, cotton cloth, etc. After the contact piece 308 absorbs the stored rinse liquid, the second telescopic rod 2905 is controlled to extend so as to drive the adsorption plate 2903 to be in pressing contact with the contact piece 308. On the basis of keeping the fourth telescopic rod 305 to be extended to the fifth length, the fourth telescopic rod 305 is further controlled to be repeatedly extended and contracted to a preset length, in the process, the fourth telescopic rod 305 repeatedly drives the contact piece 308 stored with the cleaning liquid to move through the pushing plate 307 so as to wipe and clean the lower surfaces of the two adsorption plates 2903, and further, samples adhered to the two adsorption plates 2903 are erased (because in the process that the two adsorption plates 2903 adsorb the test paper to add the samples, the samples infiltrate and permeate on the test paper, which can cause the adhesion of the test samples below the two adsorption plates 2903), so that the residual samples adhered to the two adsorption plates 2903 in the subsequent test process can be prevented from affecting the subsequent test. In the process of cleaning the two adsorption plates 2903, since the adsorption plates 2903 are in pressing contact with the contact pieces 308, cleaning of the contact pieces 308 can be completed in the process of relative movement of the two adsorption plates 2903 and the contact pieces 308 (because the contact pieces 308 are matched with the adsorption round tubes 309 in the process of conveying the test paper, samples on the test paper can be adhered to the adsorption round tubes 309 and the contact pieces 308).
Through the above-mentioned washing to parts such as two adsorption plates 2903, push plate 307, contact piece 308, adsorption circular tube 309 and test tube 17, drip plate 31, can effectively wash away remaining sample, prevent that remaining sample from to follow-up measuring influence, and then guarantee the accuracy of testing result.
Fourth embodiment
The invention also discloses a use method of the kit for detecting male infertility genes, which comprises the following steps:
step one: connecting the sample with detection with the docking liquid inlet nozzle 9, and adding the sample into the test tube 17; the jacking telescopic rod 2908 is adjusted to drive the detection test paper above the arc-shaped placing plate 2901 to move upwards, so that the uppermost detection test paper moves between the two limiting contact rollers 2902, then the second telescopic rod 2905 is adjusted to drive the adsorption plate 2903 to contact with the top of the uppermost detection test paper, the adsorption pump II 2906 is started, the adsorption pump II 2906 adsorbs the detection test paper, the second telescopic rod 2905 drives the adsorption plate 2903 to reset, and the test paper is positioned in the placing groove 26 close to the top;
step two: the metering valve 38 is opened, the fifth telescopic rod 33 drives the dropping plate 31 to move, and the detection liquid is uniformly dropped onto the detection test paper through the dropping hole 32;
step three: after the test paper is used, the first adsorption pump 311 is started, the second adsorption pump 2906 is closed, the adsorption hole 310 on the adsorption round tube 309 adsorbs the test paper, the fourth telescopic rod 305 stretches, the test paper is moved out of the box body 1 through the push-out frame 301, and collection of the test paper is completed.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (6)
1. The kit for detecting the male infertility genes comprises a box body (1) and is characterized in that a through hole is formed in one side, close to the bottom end, of the box body (1), a laminating plate (5) is connected in the through hole in a sealing manner, a hanging plate (22) is fixedly connected to the inner side, located above the through hole, of the box body (1), a first telescopic rod (21) is fixedly connected to one side, facing the through hole, of the hanging plate (22), the output end of the first telescopic rod (21) is fixedly connected with the laminating plate (5), and the laminating plate (5) is laminated with the inner wall of the through hole; the detection device is characterized in that a detection table (12) is arranged at the bottom of the box body (1), a blocking rod (13) is fixedly connected to the box body (1) close to the detection table (12), placing grooves (26) are formed in the top of the detection table (12) in an equidistant mode, replacement assemblies (29) are arranged in each placing groove (26), and pushing-out assemblies (3) are arranged at two ends of each replacement assembly (29) where the placing grooves (26) are located; the replacement assembly (29) comprises an arc-shaped placing plate (2901), the inner wall of the bottom of the placing groove (26) is fixedly connected with a jacking telescopic rod (2908), the arc-shaped placing plate (2901) is fixedly connected with the output end of the jacking telescopic rod (2908), the inner walls of the two sides of the placing groove (26) above the arc-shaped placing plate (2901) are respectively connected with a connecting shaft (2904) through bearings, the outer sides of the two connecting shafts (2904) are respectively fixedly connected with a limiting contact roller (2902), the tops of the detecting table (12) above the two limiting contact rollers (2902) are respectively fixedly connected with a second telescopic rod (2905), the output ends of the two second telescopic rods (2905) are respectively fixedly connected with an adsorption plate (2903), the bottoms of the adsorption plates (2903) are equidistantly separated from the adsorption holes (2907), the two sides of the detecting table (12) are respectively fixedly connected with side long rods (28), the tops of the side long rods (28) are respectively fixedly connected with adsorption pumps (2906), and the adsorption ends of the adsorption pumps (6) are respectively connected with the two adsorption ends of the adsorption pumps (2903) through pipelines and are respectively arranged on the two adsorption plates (2903) and the two adsorption plates (2902);
the pushing-out assembly (3) comprises a pushing-out frame (301), and a cavity is formed in the pushing-out frame (301); the outside of the laminating plate (5) and the detecting table (12) close to the placing grooves (26) are provided with mounting holes, the push-out frame (301) is fixedly connected to the inside of the two mounting holes, one side of the detecting table (12) away from the push-out frame (301) is provided with a moving hole, the mounting holes, the moving holes and the placing grooves (26) are all in a communicated state, the top of the push-out frame (301) positioned at the outer side of the box body (1) is fixedly connected with a push rod (303), the outer side of the push rod (303) facing downwards is fixedly connected with a third telescopic rod (304), the top of the push-out frame (301) positioned at the lower side of the hydraulic rod is provided with a plugging hole, the inside of the plugging hole is plugged with a plugging plate (302), and the output end of the third telescopic rod (304) is fixedly connected to the top of the plugging plate (302);
one side of the detection table (12) close to the moving hole is fixedly connected with a mounting frame (306), one side of the mounting frame (306) facing the detection table (12) is fixedly connected with a fourth telescopic rod (305), the output end of the fourth telescopic rod (305) is fixedly connected with a pushing plate (307), the top of the pushing plate (307) is fixedly connected with a contact piece (308), the outer side of the pushing plate (307) away from the mounting frame (306) is fixedly connected with an adsorption circular tube (309), the upper side of the adsorption circular tube (309) is provided with an adsorption hole (310), the outer side of the adsorption circular tube (309) is fixedly connected with a pump frame (312), the top of the pump frame (312) is fixedly connected with an adsorption pump I (311), the adsorption end of the adsorption pump I (311) is connected to the inside of the adsorption circular tube (309) through a pipeline, and the adsorption circular tube (309) is positioned below the adsorption plate (2903);
the inner wall of the box body (1) above the detection table (12) is fixedly connected with a separation plate (20), jacks are formed in the top of the separation plate (20) in an equidistant manner, test tubes (17) are inserted into the inner wall of each jack, an outer limit ring plate (35) is fixedly connected to the outer side of each test tube (17), the outer limit ring plate (35) is contacted with the top of the separation plate (20), a liquid discharge hole is formed in the bottom of each test tube (17), a liquid discharge pipe (36) is fixedly connected to the inside of each liquid discharge hole, and a metering valve (38) is connected to the outer side of each liquid discharge pipe (36) through a flange;
the bottom fixedly connected with dead lever (34) of test tube (17), and one side fixedly connected with fifth telescopic link (33) of dead lever (34) towards fluid-discharge tube (36), the output fixedly connected with drip plate (31) of fifth telescopic link (33), the bottom equidistance of drip plate (31) is left there is drip hole (32), open at the top of drip plate (31) has the connecting hole, the inside fixedly connected with butt joint hose (37) of connecting hole, the other end grafting of butt joint hose (37) is in the inside of fluid-discharge tube (36).
2. The kit for detecting male infertility genes according to claim 1, wherein one side of the box body (1) below the laminating plate (5) is fixedly connected with two support guide rails (4), the outer side of the laminating plate (5) is fixedly connected with two support sliding blocks (2), the two support sliding blocks (2) are slidably connected to the inner parts of the adjacent support guide rails (4), the top of the box body (1) is connected with a box cover (6) through a hinge, and the top of the box cover (6) is fixedly connected with a lifting handle (7).
3. The kit for detecting male infertility genes according to claim 1, wherein the outer side of the partition plate (20) below the insertion hole is fixedly connected with an outer ring frame (40), the inner side of the outer ring frame (40) is fixedly connected with an extrusion spring rod (39) at equal intervals, the inner side of the outer ring frame (40) is fixedly connected with a laminating clamping plate (41) through a hinge, the laminating clamping plate (41) is contacted with the outer side of the test tube (17), and the other end of the extrusion spring rod (39) is fixedly connected with the outer side of the laminating clamping plate (41).
4. The kit for detecting male infertility genes according to claim 1, wherein the box body (1) is fixedly connected with the same communicating pipe (14) on two side inner walls above the separation plate (20), partition holes are formed in the outer sides of the communicating pipes (14) in an equidistant manner, the inner walls of each partition hole are fixedly connected with partition pipes (27), the separation plate (20) is fixedly connected with a connecting block (19) on the top of the outer side of each test tube (17), the outer side of the connecting block (19) is fixedly connected with an exhaust pipe (18), the exhaust pipe (18) is annularly provided with an exhaust hole (30) on the outer side facing downwards, the partition pipes (27) are in a communicating state with the exhaust pipe (18), one side of the box body (1) is fixedly connected with a fixing plate (25), the top of the fixing plate (25) is fixedly connected with an exhaust pump (24), the exhaust end of the exhaust pump (24) is fixedly connected with an exhaust pipe (23), and the other end of the exhaust pipe (23) is spliced in the interior of the communicating pipe (14).
5. The kit for detecting male infertility genes according to claim 2, wherein the box cover (6) is positioned at the top of each test tube (17) and is provided with a fitting hole, two sides of the box body (1) are fixedly connected with shaft plates (42), two opposite sides of the two shaft plates (42) are connected with the same rotating shaft (16) through bearings, the outer sides of the rotating shafts (16) are fixedly connected with rotating sleeves (15) at equal intervals, the outer sides of each rotating sleeve (15) are fixedly connected with fitting plates (8), the fitting plates (8) are attached to the fitting holes, the top of each fitting plate (8) is provided with a limiting hole, the inner wall of each limiting hole is inserted with a butting liquid inlet nozzle (9), the butting liquid inlet nozzle (9) is positioned above the test tube (17), one end of each rotating shaft (16) is fixedly connected with a rotating handle (11), and the outer sides of the box body (1) positioned below the rotating sleeves (15) are fixedly connected with clamping plates (10).
6. A method of using a kit for detecting a male infertility gene as set forth in any one of claims 1 to 5, characterized in that the method comprises the steps of:
step one: connecting the sample with detection with a docking liquid inlet nozzle (9) and adding the sample into a test tube (17); the jacking telescopic rod (2908) is adjusted to drive the detection test paper above the arc-shaped placing plate (2901) to move upwards, so that the uppermost detection test paper moves between the two limiting contact rollers (2902), then the second telescopic rod (2905) is adjusted to drive the adsorption plate (2903) to contact with the top of the uppermost detection test paper, the adsorption pump II (2906) is started, the adsorption pump II (2906) adsorbs the detection test paper, the second telescopic rod (2905) drives the adsorption plate (2903) to reset, and the test paper is positioned in the placing groove (26) close to the top;
step two: the metering valve (38) is opened, the fifth telescopic rod (33) drives the dropping plate (31) to move, and the detection liquid is uniformly dropped onto the detection test paper through the liquid dropping hole (32);
step three: after the test paper is used, the first adsorption pump (311) is started, the second adsorption pump (2906) is closed, the adsorption holes (310) on the adsorption round tube (309) adsorb the test paper, the fourth telescopic rod (305) stretches, the test paper moves out of the box body (1) through the push-out frame (301), and collection of the test paper is completed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311703452.1A CN117405903B (en) | 2023-12-13 | 2023-12-13 | Kit for detecting male infertility gene and application method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311703452.1A CN117405903B (en) | 2023-12-13 | 2023-12-13 | Kit for detecting male infertility gene and application method thereof |
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| CN117405903A CN117405903A (en) | 2024-01-16 |
| CN117405903B true CN117405903B (en) | 2024-02-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202311703452.1A Active CN117405903B (en) | 2023-12-13 | 2023-12-13 | Kit for detecting male infertility gene and application method thereof |
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| CN114441761A (en) * | 2022-01-24 | 2022-05-06 | 陈香铃 | Painless blood glucose meter with automatic disinfection function |
| WO2022095043A1 (en) * | 2020-11-09 | 2022-05-12 | 沈凌云 | Gene detection kit |
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| CN115489863A (en) * | 2022-09-19 | 2022-12-20 | 宜兴市拜奥精核生物科技有限公司 | Kit for gene detection and gene detection method |
| CN115993447A (en) * | 2023-03-22 | 2023-04-21 | 苏州市药品检验检测研究中心（苏州市药品不良反应监测中心） | Colloidal gold rapid detection kit for abietic acid |
| CN117129471A (en) * | 2023-10-27 | 2023-11-28 | 深圳市明鉴检测专业技术有限公司 | Antibiotic residue detection kit and detection method thereof |
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2023
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| WO2022095043A1 (en) * | 2020-11-09 | 2022-05-12 | 沈凌云 | Gene detection kit |
| CN214539629U (en) * | 2021-04-06 | 2021-10-29 | 青岛市第八人民医院 | Gynecological infertility ovulation detection special device |
| WO2022217397A1 (en) * | 2021-04-12 | 2022-10-20 | 苏州鼎实医疗科技有限公司 | Fully automated immunoassay analyzer convenient for feeding and discharging |
| CN215116299U (en) * | 2021-05-13 | 2021-12-10 | 上海锐品生物科技有限公司 | Kit for detecting infertility antibody |
| CN114441761A (en) * | 2022-01-24 | 2022-05-06 | 陈香铃 | Painless blood glucose meter with automatic disinfection function |
| CN115489863A (en) * | 2022-09-19 | 2022-12-20 | 宜兴市拜奥精核生物科技有限公司 | Kit for gene detection and gene detection method |
| CN115993447A (en) * | 2023-03-22 | 2023-04-21 | 苏州市药品检验检测研究中心（苏州市药品不良反应监测中心） | Colloidal gold rapid detection kit for abietic acid |
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| CN117405903A (en) | 2024-01-16 |
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