CN201166670Y - Observation apparatus for biologic microfluid experiment - Google Patents
Observation apparatus for biologic microfluid experiment Download PDFInfo
- Publication number
- CN201166670Y CN201166670Y CNU2008200286297U CN200820028629U CN201166670Y CN 201166670 Y CN201166670 Y CN 201166670Y CN U2008200286297 U CNU2008200286297 U CN U2008200286297U CN 200820028629 U CN200820028629 U CN 200820028629U CN 201166670 Y CN201166670 Y CN 201166670Y
- Authority
- CN
- China
- Prior art keywords
- micro
- silicon microchip
- microscope
- fluid
- channel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses an observation device of a biotic micro-fluid experiment, and the observation device comprises a micro-injection pump, a microscope, a high-speed camera, a silicon microchip with a micro-fluid channel, a liquid waste pool and a computer. The silicon microchip is directly positioned on the workbench of the microscope; the high-speed camera is connected to the photo adapter of the microscope, and is connected with the computer through a data wire; the micro-injection pump communicates with the channel in the silicon microchip through an input hose; and the liquid waste pool communicates with the channel in the silicon microchip through an output hose. When a biotic micro-fluid injected by the injection pump flows through the channel of the silicon microchip, the fluid motion scene amplified by the microscope is shot by the high-speed camera, and then the video is transmitted to the computer through the data line for observing the flowing condition of the micro-fluid on a real-time basis and storing images.
Description
Technical field
The utility model relates to a kind of observation device of biological micro-fluid experiment.
Background technology
The main technical schemes of the biological microfluidic flow of existing observation has: the one, adopt PIV (the image particle tests the speed) mode; The ultimate principle of PIV mode is to disseminate trace particle in the flow field, with pulse laser sheet laser irradiation territory, institute flow measurement place, by continuous 2 times or multiexposure, multiple exposure, the image of particle is recorded on egative film or the CCD, absorb the frame sequence of this zone particle picture, and write down the time interval of adjacent two frame image sequence, carry out the image correlation analysis, discern the displacement of trace particle image, thereby obtain the velocity field of fluid.The 2nd, LDV (laser velocimeter) mode.The principle of work of LDV mode is that beam of laser is divided into two bundles, and the measurement point of this two bundle laser in the flow field crossed, and will form parallel interference fringe in the little space that two bundle laser intersections form.Follow particle when flowing when passing through this little space, because light scattering can take place, and then change the interference fringe form.By analyzing change of interference fringes, just can determine to follow particle's velocity.This dual mode flows owing to all having adopted trace particle to follow the tracks of fluid, so having relatively high expectations to trace particle.
Summary of the invention
Problem to be solved in the utility model provides a kind of low cost follow particle of need not to flow, simple in structure, the observation device of the biological micro-fluid experiment of reliable operation.
For reaching above purpose, the utility model relates to takes following technical scheme to be achieved:
A kind of observation device of biological micro-fluid experiment is characterized in that, comprises micro-injection pump, microscope, and high-speed camera has microfluidic channel silicon microchip, waste liquid pool and computing machine; Described silicon microchip is placed directly on the microscopical worktable; High-speed camera is connected on the microscopical photography extend neck and by data line and links to each other with computing machine; Described micro-injection pump is by the microfluidic channel UNICOM in input flexible pipe and the silicon microchip, and waste liquid pool is by the microfluidic channel UNICOM in delivery hose and the silicon microchip.
In the such scheme, described silicon microchip is a channel structure, comprises two input ends, two microfluidic channel of UNICOM respectively, and two passages are output terminal of UNICOM after the chip middle part is converted into a passage.The silicon microchip also can be the single channel structure, comprises a U-shaped microfluidic channel, respectively output terminal of UNICOM, an input end.
The utility model has the advantages that, can control the type of flow of fluid, be mobile the giving information of research microfluid (as blood) by syringe pump; Can observe the microcosmic of microfluid in silicon microchip passage clearly by trinocular microscope and flow, and can obtain studying needed video or image in real time by the computer control high-speed camera.
Description of drawings
Fig. 1 is a structural representation of the present utility model.
Fig. 2 is two kinds of structural representations of silicon microchip among Fig. 1.
Among Fig. 1 Fig. 2: 1-micro-injection pump, 2-microscope, 3-high-speed camera, 4-silicon microchip, 5-waste liquid pool, 6-computing machine; The 7-input end; The 8-microfluidic channel; The 9-output terminal.
Embodiment
Below in conjunction with drawings and Examples the utility model is described in further detail.
As shown in Figure 1: a kind of observation device of biological micro-fluid experiment comprises micro-injection pump 1, microscope 2, high-speed camera 3, silicon microchip 4, waste liquid pool 5 and control computer 6.Chip 4 is placed directly on the worktable of microscope 2, and high-speed camera 3 is connected on the photography extend neck of microscope 2 and by data line and links to each other with computing machine.
Micro-injection pump 1 is by controller, injection device, and power supply three parts constitute; Controller wherein adopts microcomputerized control, and pulse free is carried liquid reposefully, and accurately controls injection rate.During work, biological microfluid is injected in the passage of silicon microchip 4 by the input flexible pipe by syringe pump 1, flows into waste liquid pool 5 through a delivery hose then.The type of flow of microfluid generally has laminar flow and turbulent flow.Can control by the controller in the syringe pump 1.The situation that microfluid flows in chip 4 passages is amplified the back through microscope 2 photography extend necks and is taken fully by high-speed camera 3, and be sent to computing machine 6, on the display of computing machine 6, just can find out the situation of microfluidic flow so in real time clearly, and can carry out the preservation of image.
In the present embodiment, micro-injection pump 1 can adopt the ALC-IP800 type; High-speed camera 3 can adopt＞video cameras of 1000 frame/seconds; Microscope 2 can adopt the transflective industrial inspection trinocular microscope of MA2001 type.
As shown in Figure 2, silicon microchip 4 can adopt two kinds of versions, a kind of is channel structure, microfluid enters two microfluidic channel 8 through two input ends 7 respectively by two input flexible pipes, two passages 8 are converted into a passage at chip 4 middle parts, arrive waste liquid pool 5[Fig. 2 (a) through output terminal 9 by delivery hose].Another kind is the single channel structure, and microfluid enters a U-shaped microfluidic channel 8 by an input flexible pipe through input end 7, arrives waste liquid pool 5[Fig. 2 (b) through output terminal 9 by delivery hose again].
Claims (3)
1. the observation device of a biological micro-fluid experiment is characterized in that, comprises micro-injection pump, microscope, and high-speed camera has the silicon microchip of microfluidic channel, waste liquid pool and computing machine; Described silicon microchip is placed directly on the microscopical worktable; High-speed camera is connected on the microscopical photography extend neck and by data line and links to each other with computing machine; Described micro-injection pump is by the microfluidic channel UNICOM in input flexible pipe and the silicon microchip, and waste liquid pool is by the microfluidic channel UNICOM in delivery hose and the silicon microchip.
2. the observation device of biological micro-fluid experiment as claimed in claim 1, it is characterized in that, described silicon microchip is a channel structure, comprises two input ends, two microfluidic channel of UNICOM respectively, and two passages are output terminal of UNICOM after the chip middle part is converted into a passage.
3. the observation device of biological micro-fluid experiment as claimed in claim 1 is characterized in that, described silicon microchip is the single channel structure, comprises a U-shaped microfluidic channel, respectively output terminal of UNICOM, an input end.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200286297U CN201166670Y (en) | 2008-03-21 | 2008-03-21 | Observation apparatus for biologic microfluid experiment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200286297U CN201166670Y (en) | 2008-03-21 | 2008-03-21 | Observation apparatus for biologic microfluid experiment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201166670Y true CN201166670Y (en) | 2008-12-17 |
Family
ID=40192060
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008200286297U Expired - Fee Related CN201166670Y (en) | 2008-03-21 | 2008-03-21 | Observation apparatus for biologic microfluid experiment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201166670Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104597228A (en) * | 2015-01-20 | 2015-05-06 | 重庆科技学院 | Cell fluid morphologic observation chip and using method thereof |
| CN105107558A (en) * | 2015-09-14 | 2015-12-02 | 安徽博微长安电子有限公司 | Full-automatic droplet formation system and control method thereof |
| WO2020215523A1 (en) * | 2019-04-24 | 2020-10-29 | 山东科技大学 | Experiment system and experiment method based on microfluidic control and jamin effect observation |
-
2008
- 2008-03-21 CN CNU2008200286297U patent/CN201166670Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104597228A (en) * | 2015-01-20 | 2015-05-06 | 重庆科技学院 | Cell fluid morphologic observation chip and using method thereof |
| CN104597228B (en) * | 2015-01-20 | 2016-06-15 | 重庆科技学院 | A kind of cell flow morphologic observation chip and using method |
| CN105107558A (en) * | 2015-09-14 | 2015-12-02 | 安徽博微长安电子有限公司 | Full-automatic droplet formation system and control method thereof |
| WO2020215523A1 (en) * | 2019-04-24 | 2020-10-29 | 山东科技大学 | Experiment system and experiment method based on microfluidic control and jamin effect observation |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101750515B (en) | Non-contact measurement method for measuring liquid parameter | |
| CN104297108B (en) | Particle analyzer and its liquid-way system | |
| CN110044800B (en) | Flow cytometer based on microfluidic three-dimensional focusing technology | |
| Altshuler et al. | Flow-controlled densification and anomalous dispersion of E. coli through a constriction | |
| CN201166670Y (en) | Observation apparatus for biologic microfluid experiment | |
| CN110302851A (en) | Experimental system and its experimental method based on microfluidic control and Jamin effect observation | |
| CN103675333A (en) | Device and method for measuring micro-fluid velocity field in real time | |
| CN104531504B (en) | A kind of micro flow control chip device utilizing laminar flow and chemotaxis separation of bacterial | |
| CN202710481U (en) | Device for identifying and measuring air bubble in high-concentration dispersed multiphase flow | |
| US20140065658A1 (en) | Method for Monitoring A Reaction, And Reaction System For Implementing Same | |
| JP4538497B2 (en) | Flow state observation apparatus and flow state observation method | |
| CN103968900A (en) | Fluid flow measuring system and measuring method | |
| CN205133611U (en) | Continuous shearing stress generating device of cell equipartition | |
| CN104502240A (en) | Liquid parameter measurement system | |
| CN101915855B (en) | Method and device for detecting flying speeds of particles in cytometer by using secondary light-scattering method | |
| Bhattarai et al. | The total air budget in oxygenated water flowing in a drip tape irrigation pipe | |
| CN204255802U (en) | Liquid parameter measuring system | |
| CN107389502B (en) | A kind of method and system measuring liquid viscosity | |
| CN102901470B (en) | Device for measuring diameter of micro-capillary | |
| CN108982334A (en) | Cell counter and its application | |
| CN103675306B (en) | For automated analysis instrument and the system of micro-fluidic immune detection chip | |
| JP2016503507A (en) | Fluid system and method | |
| CN105547922A (en) | Microviscosimeter based on micro/nano channel | |
| JPH11118819A (en) | Measuaring method and device of flow characteristic of cell and particle | |
| Esmaeel et al. | Multi-purpose machine vision platform for different microfluidics applications |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20081217 Termination date: 20100321 |