CN205133611U - Continuous shearing stress generating device of cell equipartition - Google Patents
Continuous shearing stress generating device of cell equipartition Download PDFInfo
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- CN205133611U CN205133611U CN201520848546.2U CN201520848546U CN205133611U CN 205133611 U CN205133611 U CN 205133611U CN 201520848546 U CN201520848546 U CN 201520848546U CN 205133611 U CN205133611 U CN 205133611U
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Abstract
The utility model relates to a continuous shearing stress generating device of cell equipartition comprises microscope, fluorescence appearance, APS -C picture household video camera, parallel dull and stereotyped chamber, peristaltic pump, computer and the sample storage tank of flowing. The utility model discloses a peristaltic pump will be held real -time image transmission through the video recording equipment of installing on the microscope in will being flowed the chamber to the parallel flat board by the pump sending of survey sample to the computer, finally discern through the computer to combine the peristaltic pump flow, the ration produces the cell shearing force, and calculates the deformation of sample cell. Have the function of cell count simultaneously concurrently. The utility model is used for producing cell shearing stress and meeting an emergency to its atress and observe, receive force intensity, atress time, atress cycle that simultaneously can the pair cell be adjusted, can take into account the cell figure, activity, the density dynamic monitoring that realize the cell culture in -process in addition.
Description
Technical field
The utility model belongs to bio-science field, is specifically related to the uniform continuous shearing stress generating unit of a kind of cell.
Background technology
Cell shearing stress generator mainly contains the equipment such as atomic force microscope, micro pipette method, light tweezers, operates, involve great expense and lack statistical significance for unicellular, cell mass.Above equipment requirements operates under cell set condition simultaneously, cannot realize evenly load, distinguish to some extent with the mechanical condition of suspension microorganism and hemocyte.
The development of microfluidic control technology laser etching techniques thereupon, chip manufacture is increasingly convenient; Miniflow pump of constant delivery type universal, fluid controls to establish a capital and reaches microlitre/second rank; The development of computer vision system, real-time image procossing becomes possibility.
Now microorganism instantly, hematological cyto-mechanics research needs the uniform shearing force producer of a cheap, that processing efficiency is higher cell.
Summary of the invention
The purpose of this utility model is to propose the uniform continuous shearing stress generating unit of a kind of cell, peristaltic pump is adopted to be pumped in parallel flat flow chamber by sample, by the video recording equipment be arranged on microscope, realtime graphic is transferred to computer end, eventually through computer platform identification, thus Bound moisture pumping capacity, quantitative generation cell shearing power, and calculation sample cells deformation.Have Cytometric function concurrently simultaneously.
The utility model is for generation of cell shearing stress and observe by stress-strain it, simultaneously can regulating by force intensity, stressed time, stressed cycle cell, the cell number realized in cell cultivation process can be taken into account in addition, activity, Biomass dynamics monitor.
The uniform continuous shearing stress generating unit of the cell that the utility model proposes, is made up of microscope 1, lighting system 2, APS-C picture household video camera 3, parallel flat flow chamber 4, peristaltic pump 5, computer 6 and sample storage tank 7, wherein:
Microscope 1 is made up of microscope mirror body 11, trinocular tube 12, objective lens 13 and Stage microscope 14; Microscope mirror body 11 adopts inverted mode to arrange, the Imaging brought is vibrated to reduce water pump, trinocular tube 12 is installed on directly over microscope mirror body 11, and objective lens 13 is installed on microscope mirror body 11 microscope base, Stage microscope 14 is between trinocular tube 12, objective lens 13;
Lighting system 2 comprises luminoscope and excites mercury lamp 21, light filter group 22 and low-voltage halogen lamp 23; The light inlet of light filter group 23 aims at the light-emitting window that luminoscope excites mercury lamp 21, and luminoscope excites the light-emitting window of mercury lamp 21 to aim at the light inlet of light filter group 22, and the light-emitting window of light filter group 22 aims at Stage microscope 14;
APS-C picture household video camera 3 comprises eyepiece adapter ring 31, teleconverter 32, camera body 33 and HDMI video output line 34; The camera lens bayonet socket place of camera body 33 connects teleconverter 32, and teleconverter 32 is connected with trinocular tube 12 by eyepiece adapter ring 31, and APS-C picture household video camera 3 connects the video frequency collection card 61 of computer 6 by HDMI video output line 34; Camera body 33 captures graphic information, and is transferred on video frequency collection card 61 by HDMI video output line 34;
Parallel flat flow chamber 4 comprises parallel flat flow chamber cavity 41 and parallel flat flow chamber fixed plate 42; Parallel flat flow chamber cavity 41 is fixed on parallel flat flow chamber fixed plate 42.Parallel flat flow chamber 4 is positioned on the Stage microscope 14 of microscope 1;
Peristaltic pump 5 comprises flexible pipe import 51, the pump housing 52 and flexible pipe outlet 53; Flexible pipe import 51 clamps on the pump housing 52, and flexible pipe import 51 one end is injection port, and the other end is connected with parallel flat flow chamber cavity 41, and parallel flat flow chamber cavity 41 in addition one end and flexible pipe exports 53, as fluid outlet;
Computer 6 comprises video frequency collection card 61, cabinet 62, indicating meter 63 and input unit 64; Video frequency collection card 61 gathers household video camera 3 gained image by HDMI video output line 34.Computer 6 is equipped with indicating meter 63 and input unit 64 simultaneously;
Sample storage tank 7 comprises storage tank tank body 71, storage tank fluid inlet 72, storage tank liquid outlet 73, volumetric void fraction spiral cover 74, volumetric void fraction bar 75 and through hole 76; Storage tank tank body 71 two bottom sides is respectively equipped with storage tank fluid inlet 72 and storage tank liquid outlet 73, in storage tank tank body, bottom has the volumetric void fraction spiral cover 74 of sealing function, the through hole 76 through the center of circle is provided with bottom volumetric void fraction spiral cover 74, when volumetric void fraction spiral cover 74 is close to bottom storage tank tank body 71, through hole 76 is just to storage tank fluid inlet 72 and storage tank liquid outlet 73, and sample storage tank 7 volume is 0.Volumetric void fraction spiral cover 74 is welded with volumetric void fraction bar 75.
The beneficial effects of the utility model are:
1, device adopts APS-C picture household video camera, and effectively utilize the APS-C picture household video camera visual field in conjunction with teleconverter, and improve imaging signal to noise ratio, compare high speed, high-definition image real-time sampling function that existing microscope image pick-up facility have 1080/50P, and family expenses camera is detachable for other purposes at ordinary times.
2, visual field inner cell deformation quantity is calculated in conjunction with microscope micrometer and parallel flat flow chamber.
3, adopt luminoscope, by fluorescence excitation, the interference getting rid of abitotic substance is measured active somatic cell and activity thereof.
4, adopt video frequency collection card, and in conjunction with computer system, realize the real-time analysis to data.
5, the acellular set wall of device, produce shearing effect by fluid, shearing force is uniform, closer to blood and microorganism life shearing force condition.
6, sample storage tank has capacity regulating bar, regulates cell stressed time, stressed cycle.
7, device is by parallel flat flow chamber and peristaltic pump transmission, adopts continuous, the cycle count of target compound to carry out full sample measurement, real-time online measuring and observation.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
Fig. 2 is structure iron of the present utility model;
Number in the figure: 1 is microscope, 11 is microscope mirror body, and 12 is trinocular tube, and 13 is objective lens, and 14 is Stage microscope;
2 is lighting system, and 21 excite mercury lamp for luminoscope, and 22 is light filter group, and 23 is low-voltage halogen lamp;
3 is APS-C picture household video camera, and 31 is adapter ring on show, and 32 is teleconverter, and 33 is camera body, and 34 is HDMI video output line;
4 is parallel flat flow chamber, and 41 is parallel flat flow chamber cavity, and 42 is parallel flat flow chamber fixed plate;
5 is peristaltic pump, and 51 is flexible pipe import, and 52 is the pump housing, and 53 is flexible pipe outlet;
6 is computer, and 61 is video frequency collection card, and 62 is cabinet, and 63 is indicating meter, and 64 is input unit;
7 sample storage tanks, 71 is storage tank tank body, and 72 is storage tank fluid inlet, and 73 is storage tank liquid outlet, and 74 is volumetric void fraction spiral cover, and 75 is volumetric void fraction bar, and 76 is through hole.
Embodiment
The utility model is further illustrated by reference to the accompanying drawings below by embodiment.
Embodiment 1:
This example carries out the stressed loading of cell under low-voltage halogen lamp 23 condition.
Device comprises microscope 1, lighting system 2, APS-C picture household video camera 3, parallel flat flow chamber 4, peristaltic pump 5, computer 6 and sample storage tank 7.
Microscope 1 is based on microscope mirror body 11, inverted microscope is adopted to vibrate to reduce water pump the Imaging brought, trinocular tube 12 is installed on directly over microscope mirror body 11, and objective lens 13 is installed on microscope mirror body 11 microscope base, Stage microscope 14 is between trinocular tube 12, objective lens 13.
Lighting system 2 is the optional feature of microscope 1, and lighting system 2 is installed on microscopical rear portion.Lighting system 2 excites mercury lamp 21 and low-voltage halogen lamp 23 based on luminoscope, and light filter group 23 is installed in luminoscope light path.The excitation beam that luminoscope excites mercury lamp 21 to send out is transmitted on Stage microscope 14 by the light filter group 22 in luminoscope light path.
APS-C picture household video camera 3 is based on camera body 33, and the camera lens bayonet socket place of camera body 33 connects teleconverter 32, and teleconverter 32 is crossed eyepiece adapter ring 31 and is connected with trinocular tube 12.Camera body 33 captures graphic information, and is transferred on video frequency collection card 61 by HDMI video output line 34.
Parallel flat flow chamber 4 is based on parallel flat flow chamber cavity 41, and parallel flat flow chamber cavity 41 is fixed on parallel flat flow chamber fixed plate 42.Parallel flat flow chamber 4 is positioned on the Stage microscope 14 of microscope 1.The flexible pipe import 51 of peristaltic pump 5 clamps on the pump housing 52, and flexible pipe import 51 one end is injection port, and the other end is connected with parallel flat flow chamber cavity 41, and parallel flat flow chamber cavity 41 in addition one end and flexible pipe exports 53, as fluid outlet.
Computer 6 comprises based on cabinet 62, and installs video frequency collection card 61 additional, and video frequency collection card 61 gathers household video camera 3 gained image by HDMI video output line 34.Computer 6 is equipped with indicating meter 63 and input unit 64 simultaneously.
Sample storage tank 7 is based on storage tank tank body 71, and tank base both sides have respectively: storage tank fluid inlet 72 and storage tank liquid outlet 73, has the volumetric void fraction spiral cover 74 with sealing function above tank base in tank body.Have the through hole 76 through the center of circle bottom volumetric void fraction spiral cover 74, when volumetric void fraction spiral cover 74 is close to bottom storage tank tank body 71, through hole 76 is just to storage tank fluid inlet 72 and storage tank liquid outlet 73, and sample storage tank 7 volume is 0.Volumetric void fraction spiral cover 74 is welded with volumetric void fraction bar 75, can spiral cover be rotated, regulate sample storage tank 7 volume.
According to shown in following table, sample frequency and flow velocity are set in conjunction with the objective lens 13 object lens enlargement ratio that works:
| Frequency (Hz) | 60 | 30 | 60 | 30 | 60 | 30 |
| Enlargement ratio | 40 | 40 | 20 | 20 | 10 | 10 |
| Lowest speed (micron/s) | 3 | 2 | 6 | 4 | 12 | 8 |
View data is read in from video frequency collection card 61.
Run the pump housing 52 of peristaltic pump 5, emptying inner air tube, conveying sample.Sample storage tank 7 volume V, consistent flow Q, now the stressed cycle of liquid is T=V/Q, and volume adjusted controls the volumetric void fraction bar 75 on spiral cover 74, rotates spiral cover, is adjusted to the required stressed cycle.If be omnidistance stressed, then volumetric void fraction spiral cover 74 rotated and be adjusted to bottom sample storage tank 7.
The image obtained by computer 6 calculates shearing force size, and regulates.
Terminate to measure, close all appts with after the clear pipeline of distilled water.Take off parallel flat flow chamber 4, be separated the flexible pipe import 51 of peristaltic pump 5, the pump housing 52, flexible pipe outlet 53, sample storage tank 9, clean, dry and treat to use next time.APS-C picture household video camera 3 is taken off from trinocular tube 12, is separated with camera body 33, teleconverter 32, eyepiece adapter ring 31 and HDMI video output line 34, concentrates folding and unfolding.
Embodiment 2:
This example carries out the stressed loading of cell under luminoscope excites mercury lamp 21 condition.
APS-C picture household video camera 3 is connected on teleconverter 32 with the camera lens bayonet socket place of camera body 33, and teleconverter 32 is connected with trinocular tube 12 by eyepiece adapter ring 31, HDMI video output line 34 on camera body 33 connects.Open the low-voltage halogen lamp 23 in lighting system 2, open computer 6, microscope micrometer 8 is positioned over Stage microscope 14, just to work objective lens optical axis to objective lens 13.To regulate the microscope mirror body 11 of display microscope 1, the object lens that make to work in objective lens 13 correctly focus on.
The flexible pipe import 51 of the parallel flat flow chamber cavity 41 of parallel flat flow chamber 4 and peristaltic pump 5, flexible pipe are exported 53 and be connected, flexible pipe import 51, flexible pipe outlet 53 other one end connect storage tank fluid inlet 72 and storage tank liquid outlet 73 respectively, flexible pipe import 51 are fastened on the pump housing 52.By parallel flat flow chamber 4, be placed on Stage microscope 14 also fixing.
Close low-voltage halogen lamp 23, open luminoscope and excite mercury lamp 21, preheating 10min.
According to shown in following table, sample frequency and flow velocity are set in conjunction with the objective lens 13 object lens enlargement ratio that works:
| Frequency (Hz) | 60 | 30 | 60 | 30 | 60 | 30 |
| Enlargement ratio | 40 | 40 | 20 | 20 | 10 | 10 |
| Peak flow rate (micron/s) | 4 | 3 | 8 | 6 | 16 | 12 |
View data is read in from video frequency collection card 61.
Run peristaltic pump 5 pump housing 52, emptying inner air tube, conveying sample.Sample storage tank 7 volume V, consistent flow Q, now the stressed cycle of liquid is T=V/Q, and volume adjusted controls the volumetric void fraction bar 75 on spiral cover 74, rotates spiral cover, is adjusted to the required stressed cycle.If be omnidistance stressed, then volumetric void fraction spiral cover 74 rotated and be adjusted to bottom sample storage tank 7.
The image obtained by computer 6 calculates shearing force size, and regulates.
Terminate to measure, close all appts with after the clear pipeline of distilled water.Take off parallel flat flow chamber 4, be separated the flexible pipe import 51 of peristaltic pump 5, the pump housing 52, flexible pipe outlet 53, sample storage tank 7, clean, dry and treat to use next time.APS-C picture household video camera 3 is taken off from trinocular tube 12, is separated with camera body 33, teleconverter 32, eyepiece adapter ring 31 and HDMI video output line 34, concentrates folding and unfolding.
Claims (1)
1. the uniform continuous shearing stress generating unit of cell, be made up of microscope (1), lighting system (2), APS-C picture household video camera (3), parallel flat flow chamber (4), peristaltic pump (5), computer (6) and sample storage tank (7), it is characterized in that:
Microscope (1) is made up of microscope mirror body (11), trinocular tube (12), objective lens (13) and Stage microscope (14); Microscope mirror body (11) adopts inverted mode to arrange, the Imaging brought is vibrated to reduce water pump, trinocular tube (12) is installed on directly over microscope mirror body (11), and objective lens (13) is installed on microscope mirror body (11) microscope base, Stage microscope (14) is positioned at trinocular tube (12), between objective lens (13);
Lighting system (2) comprises luminoscope and excites mercury lamp (21), light filter group (22) and low-voltage halogen lamp (23); The light inlet of light filter group (23) aims at the light-emitting window that luminoscope excites mercury lamp (21), luminoscope excites the light-emitting window of mercury lamp (21) to aim at the light inlet of light filter group (22), and the light-emitting window of light filter group (22) aims at Stage microscope (14);
APS-C picture household video camera (3) comprises eyepiece adapter ring (31), teleconverter (32), camera body (33) and HDMI video output line (34); The camera lens bayonet socket place of camera body (33) connects teleconverter (32), teleconverter (32) is connected with trinocular tube (12) by eyepiece adapter ring (31), and APS-C picture household video camera (3) connects the video frequency collection card (61) of computer (6) by HDMI video output line (34); Camera body (33) captures graphic information, and is transferred on video frequency collection card (61) by HDMI video output line (34);
Parallel flat flow chamber (4) comprises parallel flat flow chamber cavity (41) and parallel flat flow chamber fixed plate (42); Parallel flat flow chamber cavity (41) is fixed on parallel flat flow chamber fixed plate (42), and parallel flat flow chamber (4) is positioned on the Stage microscope (14) of microscope (1);
Peristaltic pump (5) comprises flexible pipe import (51), the pump housing (52) and flexible pipe outlet (53); Flexible pipe import (51) clamps on the pump housing (52), flexible pipe import (51) one end is injection port, the other end is connected with parallel flat flow chamber cavity (41), parallel flat flow chamber cavity (41) in addition one end and flexible pipe exports (53), as fluid outlet;
Computer (6) comprises video frequency collection card (61), cabinet (62), indicating meter (63) and input unit (64); Video frequency collection card (61) gathers household video camera (3) gained image by HDMI video output line (34), and computer (6) is equipped with indicating meter (63) and input unit (64) simultaneously;
Sample storage tank (7) comprises storage tank tank body (71), storage tank fluid inlet (72), storage tank liquid outlet (73), volumetric void fraction spiral cover (74), volumetric void fraction bar (75) and through hole (76), storage tank tank body (71) two bottom sides is respectively equipped with storage tank fluid inlet (72) and storage tank liquid outlet (73), in storage tank tank body, bottom has the volumetric void fraction spiral cover (74) of sealing function, volumetric void fraction spiral cover (74) bottom is provided with the through hole (76) through the center of circle, when volumetric void fraction spiral cover (74) is close to storage tank tank body (71) bottom, through hole (76) is just to storage tank fluid inlet (72) and storage tank liquid outlet (73), sample storage tank (7) volume is 0, volumetric void fraction spiral cover (74) is welded with volumetric void fraction bar (75).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520848546.2U CN205133611U (en) | 2015-10-30 | 2015-10-30 | Continuous shearing stress generating device of cell equipartition |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520848546.2U CN205133611U (en) | 2015-10-30 | 2015-10-30 | Continuous shearing stress generating device of cell equipartition |
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| CN205133611U true CN205133611U (en) | 2016-04-06 |
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| CN201520848546.2U Expired - Fee Related CN205133611U (en) | 2015-10-30 | 2015-10-30 | Continuous shearing stress generating device of cell equipartition |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105974102A (en) * | 2016-04-28 | 2016-09-28 | 新乡医学院 | Apparatus for observing shear stress adjusted PCSK9 expression |
| CN106119079A (en) * | 2016-07-06 | 2016-11-16 | 四川大学 | Clearance flow cyto-mechanics biology experimental installation between one |
| CN108398371A (en) * | 2018-02-09 | 2018-08-14 | 浙江大学 | A kind of reproducting method of the analysis and Rheologic of haemocyte shear stress rheology observation device and haemocyte shear stress Rheological Regularity |
| CN109655379A (en) * | 2018-12-29 | 2019-04-19 | 潍坊医学院 | For Study of Fluid shear stress to the chute panel assembly and measuring method of impact cell |
| CN110161669A (en) * | 2019-06-12 | 2019-08-23 | 中国科学院自动化研究所 | Based on analog video signal in body fluorescence microscopy lens head and fluorescence microscope |
| CN111057640A (en) * | 2019-12-09 | 2020-04-24 | 西北工业大学 | Experimental device for in-situ research cell mechanical characteristics |
-
2015
- 2015-10-30 CN CN201520848546.2U patent/CN205133611U/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105974102A (en) * | 2016-04-28 | 2016-09-28 | 新乡医学院 | Apparatus for observing shear stress adjusted PCSK9 expression |
| CN106119079A (en) * | 2016-07-06 | 2016-11-16 | 四川大学 | Clearance flow cyto-mechanics biology experimental installation between one |
| CN108398371A (en) * | 2018-02-09 | 2018-08-14 | 浙江大学 | A kind of reproducting method of the analysis and Rheologic of haemocyte shear stress rheology observation device and haemocyte shear stress Rheological Regularity |
| CN109655379A (en) * | 2018-12-29 | 2019-04-19 | 潍坊医学院 | For Study of Fluid shear stress to the chute panel assembly and measuring method of impact cell |
| CN109655379B (en) * | 2018-12-29 | 2022-02-01 | 潍坊医学院 | Chute plate device and measuring method for researching influence of fluid shear stress on cells |
| CN110161669A (en) * | 2019-06-12 | 2019-08-23 | 中国科学院自动化研究所 | Based on analog video signal in body fluorescence microscopy lens head and fluorescence microscope |
| CN111057640A (en) * | 2019-12-09 | 2020-04-24 | 西北工业大学 | Experimental device for in-situ research cell mechanical characteristics |
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| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160406 Termination date: 20181030 |
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| CF01 | Termination of patent right due to non-payment of annual fee |