CN110161669A - Based on analog video signal in body fluorescence microscopy lens head and fluorescence microscope - Google Patents
Based on analog video signal in body fluorescence microscopy lens head and fluorescence microscope Download PDFInfo
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- CN110161669A CN110161669A CN201910504965.7A CN201910504965A CN110161669A CN 110161669 A CN110161669 A CN 110161669A CN 201910504965 A CN201910504965 A CN 201910504965A CN 110161669 A CN110161669 A CN 110161669A
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- 238000000799 fluorescence microscopy Methods 0.000 title claims abstract description 25
- 230000003287 optical effect Effects 0.000 claims abstract description 71
- 238000006243 chemical reaction Methods 0.000 claims abstract description 36
- 238000003384 imaging method Methods 0.000 claims description 23
- 238000001914 filtration Methods 0.000 claims description 7
- 230000005284 excitation Effects 0.000 claims description 6
- 241001025261 Neoraja caerulea Species 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 abstract description 5
- 238000009434 installation Methods 0.000 abstract description 2
- 230000005540 biological transmission Effects 0.000 description 7
- 239000008280 blood Substances 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 210000004556 brain Anatomy 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 238000002599 functional magnetic resonance imaging Methods 0.000 description 3
- 230000013011 mating Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 244000144985 peep Species 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241001062009 Indigofera Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000029918 bioluminescence Effects 0.000 description 1
- 238000005415 bioluminescence Methods 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000005331 crown glasses (windows) Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000005308 flint glass Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000000004 hemodynamic effect Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 238000002610 neuroimaging Methods 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 238000002601 radiography Methods 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 210000000278 spinal cord Anatomy 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 230000026676 system process Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
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- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Engineering & Computer Science (AREA)
- Multimedia (AREA)
- Microscoopes, Condenser (AREA)
Abstract
The present invention relates to microscope technology fields, and in particular to it is a kind of based on analog video signal in body fluorescence microscopy lens head and fluorescence microscope, it is intended in order to solve the problems, such as that volume, weight are big and installation is inconvenient.What it is the present invention is based on analog video signal includes exciting light sources, optical path conversion mirror, gradient refractive index object lens, analog video camera mould group and video frequency collection card in body fluorescence microscopy lens head, in which: exciting light sources are connected to gradient refractive index object lens by optical path conversion mirror;Gradient refractive index object lens are connected to analog video camera mould group by optical path conversion mirror;Video frequency collection card is connect with analog video camera module signal, for acquiring the vision signal of analog video camera mould group output.Fluorescence microscopy lens head overall volume of the invention is small, light-weight, mountable to be connected with the lesser living body of figure (such as mouse) using flexible cable, does not influence it and move freely.
Description
Technical field
The present invention relates to microscope fields, and in particular to it is a kind of based on analog video signal in body fluorescence microscopy lens head
And fluorescence microscope.
Background technique
The micro- based endoscopic imaging technology of brain is a kind of emerging bioluminescence imaging technology.It not only can be by quick to intracerebral calcium
The fluorescent marker for feeling albumen is realized to the movable high-speed record of cellular level single unit discharge, and in biggish visual field model
There is good spatial resolution in enclosing, good spatial information can be obtained.It can also be by blood red egg in addition to this
White label is realized and carries out real time imagery to blood vessel structure and blood microcirculation variation in cellular level.These technological merits
If BOLD-FMRI can be unfolded in prompt under magnetic resonance environment, (Blood oxygen level dependence functional mri, full name in English are
Functional magnetic resonance imaging of blood oxygen level dependent is a kind of
Emerging neuroimaging mode, principle are that shaken radiography using magnetic to measure that neuron activity causes hemodynamic is changed
Become.Due to the Noninvasive of fMRI, without the application relatively broad with it of radioactive exposure problem, just in brain since generation nineteen ninety
Portion functional localization field occupies a tiny space.Presently mainly be used in the brain or spinal cord of research people and animal) with brain is micro- interior peeps
Technology synchronous imaging will bring breakthrough progress to the research of the neuromechanism of BOLD blood oxygen signal.
Currently, usually by imaging sensor at picture be directly used in observation, it is big with volume, weight, body can not be mounted on
The lesser living body (such as mouse) of product can not move freely.Therefore there is an urgent need to a kind of fluorescence microscopy lens head and fluorescence microscopies
Mirror, with solve or at least mitigate the above problem.
Summary of the invention
In order to solve the above problem in the prior art, traditional light is arranged in order to solve no enough height
Peep in declining, the embodiment of the present invention on the one hand provide it is a kind of based on analog video signal in body fluorescence microscopy lens head, packet
Include exciting light sources, optical path conversion mirror, gradient refractive index object lens, analog video camera mould group and video frequency collection card, in which:
The exciting light sources are connected to the gradient refractive index object lens by the optical path conversion mirror, are swashed with being formed for described
The excitation optical channel that the light that illuminating source issues passes through;
The gradient refractive index object lens are connected to the analog video camera mould group by the optical path conversion mirror, with formed at
The imaging optical channel passed through as the fluorescence that target issues;
The video frequency collection card is connect with the analog video camera module signal, acquires the analog video camera mould group output
Vision signal;
The video frequency collection card is connect with the analog video camera mould group by flexible cable.
In some preferred embodiments, the optical path conversion mirror is total reflection prism or dichroscope.
In some preferred embodiments, the first filter is provided between the optical path conversion mirror and the analog video camera mould group
Mating plate, for filtering out the stray light from the imaging that the optical path conversion mirror is transmitted to the analog video camera mould group.
It in some preferred embodiments, further include LED drive circuit, the exciting light sources are LED light source, the LED
Driving circuit and the LED light source are electrically connected, to drive the LED light source to shine and provide overcurrent protection.
In some preferred embodiments, further include the second optical filter, second optical filter setting the LED light source with
Between the optical path conversion mirror, for filtering out the stray light in LED light source.
It in some preferred embodiments, further include achromatic lens, the achromatic lens is arranged in described first and filters
Between piece and the analog video camera mould group, for the imaging to the analog video camera mould group is transmitted to from the optical path conversion mirror
The color difference of light is corrected and imaging is passed to the analog video camera mould group.
In some preferred embodiments, the LED light source is blue-ray LED diode, and first optical filter is green filter
Mating plate, second optical filter are blue color filter.
In some preferred embodiments, the analog video camera mould group is B/W camera or colour TV camera.
Second aspect of the embodiment of the present invention provides a kind of fluorescence microscope, comprising:
Bracket;
It is any it is above-mentioned based on analog video signal in body fluorescence microscopy lens head, the exciting light sources, the light
Road conversion mirror, the gradient refractive index object lens, the analog video camera mould group and the video frequency collection card are mounted on the bracket.
In some preferred embodiments, fluorescence microscope further includes host computer, the host computer and the video frequency collection card
Signal connection, for being stored and/or being analyzed based on receiving the image from the video frequency collection card, the host computer with
The LED drive circuit is electrically electrically connected using flexible cable, for LED drive circuit power supply.
The invention has the benefit that
Exciting light sources, to optical path conversion mirror, are transferred to gradient under the action of optical path conversion mirror through excitation optical channel transmission
Imageable target surface is irradiated to after refractive objective lens, imageable target issues fluorescence, and then the fluorescence is along imaging optical channel (successively through ladder
Degree refractive objective lens, optical path conversion mirror are transferred to analog video camera mould group) transmission, analog signal is generated through analog video camera mould group
Video image, picture signal are transmitted to video frequency collection card, are finally stored, are analyzed by host computer;Overall volume is small, weight
Gently, mountable to be connected with the lesser living body of figure (such as mouse) using flexible cable, it is not influenced move freely.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of an embodiment of fluorescence microscopy lens head.
Appended drawing reference and explanation:
1, analog video camera mould group;2, achromatic lens;3, the first optical filter;4, optical path conversion mirror;5, gradient refractive index object
Mirror;6, the second optical filter;7, exciting light sources;8, LED drive circuit;9, host computer;10, video frequency collection card;11, cable.
Specific embodiment
To keep the embodiment of the present invention, technical solution and advantage more obvious, below in conjunction with attached drawing to skill of the invention
Art scheme carries out clear, complete description, it is clear that and the embodiment described is a part of the embodiments of the present invention, rather than all
Embodiment.It will be apparent to a skilled person that these embodiments are used only for explaining technical principle of the invention, and
It is not intended to be limiting protection scope of the present invention.
The embodiment of the present invention on the one hand disclose it is a kind of based on analog video signal in body fluorescence microscopy lens head, referring to
Fig. 1 comprising exciting light sources 7, optical path conversion mirror 4, gradient refractive index object lens 5, analog video camera mould group 1 and video frequency collection card
10, in which:
The exciting light sources are connected to the gradient refractive index object lens by the optical path conversion mirror, are swashed with being formed for described
The excitation optical channel that the light that illuminating source issues passes through;
The gradient refractive index object lens are connected to the analog video camera mould group by the optical path conversion mirror, with formed at
The imaging optical channel passed through as the fluorescence that target issues;
The video frequency collection card is connect with the analog video camera module signal, for acquiring the analog video camera mould group
The vision signal of output;
The video frequency collection card is connect with the analog video camera mould group by flexible cable.
Exciting light sources, to optical path conversion mirror, are transferred to gradient under the action of optical path conversion mirror through excitation optical channel transmission
Imageable target surface is irradiated to after refractive objective lens, imageable target issues fluorescence, and then the fluorescence is along imaging optical channel (successively through ladder
Degree refractive objective lens, optical path conversion mirror are transferred to analog video camera mould group) transmission, analog signal is generated through analog video camera mould group
Video image, picture signal are transmitted to video frequency collection card, are finally stored, analyzed by host computer 9;Overall volume is small, weight
Gently, mountable to be connected with the lesser living body of figure (such as mouse) using flexible cable, it is not influenced move freely.
Optical path conversion mirror, dichroscope almost penetrate the light of certain wavelength, and almost to the light of other wavelength
It is fully reflective, exciting light is coupled on gradient refractive index object lens in the way of reflection by this characteristic, and allow imaging edge
Gradient refractive index object lens are transmitted to analog video camera mould group.Several concrete types of optical path conversion mirror can be total reflection prism and two
To one of Look mirror.Those of skill in the art would appreciate that it can be also other types, this will not be repeated here, as long as its energy
Realize that light path converting (can guarantee exciting light along excitation optical channel transmission, imaging along imaging optical channel transmission).In addition, complete
Reflex prism and dichroscope realize total reflection using anaclasis principle.
In addition, being provided with the first optical filter 3 between optical path conversion mirror and analog video camera mould group, turn for filtering out from optical path
The stray light that mirror is transmitted in the imaging of analog video camera mould group is changed, monochromatic light (the such as first optical filtering positioned at a certain wave band is only made
When piece is green color filter, which is green light) pass through, imaging effect is improved, is conducive to improve image quality.
In addition, may also include achromatic lens 2 in body fluorescence microscopy lens head based on analog video signal, achromatism is saturating
Mirror is arranged between the first optical filter and analog video camera mould group, for being transmitted to analog video camera mould group to from optical path conversion mirror
The color difference of imaging is corrected and imaging is passed to analog video camera mould group.Achromatic lens is by two kinds of optical property differences
Glass made of convex, concavees lens be bonded.Common refractive index is smaller and convex lens is made in the biggish crown glass of dispersive power,
It is larger with refractive index and concavees lens are made in the lesser flint glass of dispersive power.Achromatic lens generally can only be being considered main
The picture point for the two kinds of coloured light wanted coincides together, to eliminate the color difference of both coloured light.For example, for visual instrument,
In view of directly being observed with eyes, C line (feux rouges that 656.3 nanometers of wavelength) and the F line (indigo plant that 486.1 nanometers of wavelength can be eliminated
Light) color difference;For photographic film, from the aspect of photochemistry, D line (yellow lights that 589.3 nanometers of wavelength) and G line can be eliminated
The color difference of (purple lights that 430.8 nanometers of wavelength).Though still having residual achromatic aberration to other coloured light, influenced not for being normally applied
Greatly, it is believed that substantially eliminate color difference.
It should be noted that may also include LED drive circuit in body fluorescence microscopy lens head based on analog video signal
8, exciting light sources are LED light source, and LED drive circuit and LED light source are electrically connected, to drive LED light source to shine and provided
Stream protection.LED light source have the service life is long, luminous intensity is big, it is at low cost etc. it is many a little, meanwhile, when using LED light source, also
It may include the LED drive circuit being electrically connected with LED light source, to drive LED light source to shine, so that LED light source work is steady
It is fixed, reduce influence of the power supply circuit fluctuation to LED light source, LED drive circuit can also provide overcurrent guarantor for the work of LED power
Shield.LED drive circuit is well-known technique in those skilled in the art, is not repeated to illustrate.
It should be noted that the camera lens may also include the second optical filter 6, the setting of the second optical filter LED light source with it is described
Between optical path conversion mirror, for filtering out the stray light in LED light source., LED light source is blue-ray LED diode, and the first optical filter is
Green color filter, the second optical filter are blue color filter.LED diode, which issues, contains part stray light in blue light, second filters
Sector-meeting filters the stray light, so that only blue light can be by the second optical filter, imageable target is in blue light illumination green-emitting next time
Fluorescence, the first optical filter filter out after the stray light in fluorescence that only green light can pass through, and further improve image quality.Certainly,
Those of skill in the art would appreciate that exciting light can also use other colors, and it can accordingly adjust the second optical filter, the first filter
The type of mating plate, does not enumerate herein.
It should be noted that analog video camera mould group can be B/W camera, or colour TV camera.
Second aspect of the embodiment of the present invention discloses a kind of fluorescence microscope, on which includes bracket and is any
State based on analog video signal in body fluorescence microscopy lens head, say on the whole, exciting light sources, optical path conversion mirror, gradient
Refractive objective lens, analog video camera mould group and video frequency collection card are mounted on bracket.Certainly, bracket is first party of the embodiment of the present invention
Each component of the fluorescence microscopy lens head in face provides installation foundation.
Inventors have found that sometimes needing to the storage of fluorescence microscope imaging and post analysis, therefore, the present invention is real
Applying fluorescence microscope disclosed in a second aspect further includes host computer, and host computer is connect with Image Acquisition card signal, is used for base
It being stored and/or is analyzed in receiving the image from image pick-up card, host computer and LED drive circuit are electrically connected, with
USB interface is connected to by cable 11 for LED drive circuit power supply host computer, LED drive circuit and image pick-up card are all connected with
To USB interface, so that the picture signal of image pick-up card can pass through USB interface and cable transmission to host computer, host computer
Power supply module (such as power supply) can by cable and USB interface be LED drive circuit power.The host computer can be PC
(Personal Computer) or other smart machines (such as intelligent mobile terminal).
It should be noted that in the description of the present invention, term " center ", "upper", "lower", "left", "right", "vertical",
"horizontal", "inner", "outside" etc. indicate that the term of direction or positional relationship is direction based on the figure or positional relationship, this is only
It is merely for convenience of describing, rather than indication or suggestion described device or element must have a particular orientation, with specific side
Position construction and operation, therefore be not considered as limiting the invention.In addition, term " first ", " second ", " third " are only used for
Purpose is described, relative importance is not understood to indicate or imply.
In addition it is also necessary to explanation, in the description of the present invention unless specifically defined or limited otherwise, term " peace
Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally
Connection;It can be mechanical connection, be also possible to be electrically connected;Can be directly connected, can also indirectly connected through an intermediary,
It can be the connection inside two elements.To those skilled in the art, it can understand that above-mentioned term exists as the case may be
Concrete meaning in the present invention.
Term " includes " or any other like term are intended to cover non-exclusive inclusion, so that including a system
Process, article or the equipment/device of column element not only include those elements, but also other are wanted including what is be not explicitly listed
Element either further includes these processes, article or the intrinsic element of equipment/device.
So far, it has been combined preferred embodiment shown in the drawings and describes technical solution of the present invention, still, this field
Technical staff is it is easily understood that protection scope of the present invention is expressly not limited to these specific embodiments.Without departing from this
Under the premise of the principle of invention, those skilled in the art can make equivalent change or replacement to the relevant technologies feature, these
Technical solution after change or replacement will fall within the scope of protection of the present invention.
Claims (10)
1. it is a kind of based on analog video signal in body fluorescence microscopy lens head, which is characterized in that including exciting light sources, optical path
Conversion mirror, gradient refractive index object lens, analog video camera mould group and video frequency collection card, in which:
The exciting light sources are connected to the gradient refractive index object lens by the optical path conversion mirror, supply the exciting light to be formed
The excitation optical channel that the light that light source issues passes through;
The gradient refractive index object lens are connected to the analog video camera mould group by the optical path conversion mirror, to be formed for mesh is imaged
The imaging optical channel that the fluorescence that mark issues passes through;
The video frequency collection card is connect with the analog video camera module signal, for acquiring the analog video camera mould group output
Vision signal;
The video frequency collection card is connect with the analog video camera mould group by flexible cable.
2. it is according to claim 1 based on analog video signal in body fluorescence microscopy lens head, which is characterized in that it is described
Optical path conversion mirror is total reflection prism or dichroscope.
3. it is according to claim 1 or 2 based on analog video signal in body fluorescence microscopy lens head, which is characterized in that
It is provided with the first optical filter between the optical path conversion mirror and the analog video camera mould group, for filtering out from the light path converting
Mirror is transmitted to the stray light in the imaging of the analog video camera mould group.
4. it is according to claim 3 based on analog video signal in body fluorescence microscopy lens head, which is characterized in that also wrap
LED drive circuit is included, the exciting light sources are LED light source, and the LED drive circuit and the LED light source are electrically connected, with
The LED light source is driven to shine and provide overcurrent protection.
5. it is according to claim 4 based on analog video signal in body fluorescence microscopy lens head, which is characterized in that also wrap
The second optical filter is included, second optical filter is arranged between the LED light source and the optical path conversion mirror, for filtering out LED
Stray light in light source.
6. it is according to claim 5 based on analog video signal in body fluorescence microscopy lens head, which is characterized in that also wrap
Achromatic lens is included, the achromatic lens is arranged between first optical filter and the analog video camera mould group, is used for
The color difference for the imaging for being transmitted to the analog video camera mould group from the optical path conversion mirror is corrected and passes to imaging
The analog video camera mould group.
7. it is according to claim 4 based on analog video signal in body fluorescence microscopy lens head, which is characterized in that it is described
LED light source is blue-ray LED diode, and first optical filter is green color filter, and second optical filter is blue color filter.
8. it is according to claim 4 based on analog video signal in body fluorescence microscopy lens head, which is characterized in that it is described
Analog video camera mould group is B/W camera or colour TV camera.
9. a kind of fluorescence microscope characterized by comprising
Bracket;And
Described in any one of claims 1 to 9 based on analog video signal in body fluorescence microscopy lens head, the exciting light
Light source, the optical path conversion mirror, the gradient refractive index object lens, the analog video camera mould group and the video frequency collection card are respectively mounted
In the bracket.
10. fluorescence microscope according to claim 9, which is characterized in that further include host computer, the host computer with it is described
The connection of video acquisition card signal, for being stored and/or being analyzed based on receiving the image from the video frequency collection card, institute
It states host computer and is electrically connected with the LED drive circuit using flexible cable, for LED drive circuit power supply.
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CN101915754A (en) * | 2010-05-19 | 2010-12-15 | 上海理工大学 | Double-path frequency-division multiplexing fluorescent confocal microscopic imaging system and implementation method |
CN101949685A (en) * | 2010-09-08 | 2011-01-19 | 南京师范大学 | Fiber laser self-mixing interferometer and measurement method thereof |
CN105044069A (en) * | 2015-08-12 | 2015-11-11 | 合肥源信智能仪器有限公司 | Laser fluorescence inspection method for recognizing sequence of crossed writing and seal stamp |
CN205133611U (en) * | 2015-10-30 | 2016-04-06 | 同济大学 | Continuous shearing stress generating device of cell equipartition |
CN108732739A (en) * | 2018-07-25 | 2018-11-02 | 中国科学院苏州生物医学工程技术研究所 | The system being imaged in body multidigit point is realized using fiber optic bundle |
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2019
- 2019-06-12 CN CN201910504965.7A patent/CN110161669A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101915754A (en) * | 2010-05-19 | 2010-12-15 | 上海理工大学 | Double-path frequency-division multiplexing fluorescent confocal microscopic imaging system and implementation method |
CN101949685A (en) * | 2010-09-08 | 2011-01-19 | 南京师范大学 | Fiber laser self-mixing interferometer and measurement method thereof |
CN105044069A (en) * | 2015-08-12 | 2015-11-11 | 合肥源信智能仪器有限公司 | Laser fluorescence inspection method for recognizing sequence of crossed writing and seal stamp |
CN205133611U (en) * | 2015-10-30 | 2016-04-06 | 同济大学 | Continuous shearing stress generating device of cell equipartition |
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Application publication date: 20190823 |