CN104597228A - Cell fluid morphologic observation chip and using method thereof - Google Patents
Cell fluid morphologic observation chip and using method thereof Download PDFInfo
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- CN104597228A CN104597228A CN201510027343.1A CN201510027343A CN104597228A CN 104597228 A CN104597228 A CN 104597228A CN 201510027343 A CN201510027343 A CN 201510027343A CN 104597228 A CN104597228 A CN 104597228A
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- 230000000877 morphologic effect Effects 0.000 title claims abstract description 17
- 239000012530 fluid Substances 0.000 title claims abstract description 10
- 238000000034 method Methods 0.000 title claims abstract description 9
- 239000000523 sample Substances 0.000 claims description 77
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 230000001413 cellular effect Effects 0.000 claims description 4
- 239000012488 sample solution Substances 0.000 claims description 3
- 230000000007 visual effect Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 abstract description 2
- 239000000945 filler Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 15
- 238000011160 research Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000010923 batch production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 238000009534 blood test Methods 0.000 description 1
- 210000000746 body region Anatomy 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 208000014951 hematologic disease Diseases 0.000 description 1
- 238000013160 medical therapy Methods 0.000 description 1
- 238000010606 normalization Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a cell fluid morphologic observation chip. The cell fluid morphologic observation chip is characterized in that: a sample introduction main channel is processed at one end of a rectangular chip body, the end of the sample introduction main channel is provided with three outlets which are connected with three straight sample introduction branched paths, the outlet ends of the three straight sample introduction branched paths are perpendicular to the long side of the chip body in a leveling manner, are sequentially connected with an observation channel I, an observation channel II and an observation channel III, of which the widths are reduced, and are converged at a sample outlet main channel, a sample outlet main channel terminal is connected with a sample outlet, and the above structure forms an observation unit; the chip has three specifications according to the quantity of 1-3 observation units. The invention also provides a using method of the cell fluid morphologic observation chip. The cell fluid morphologic observation chip has the technical characteristics that the cell fluid morphologic observation chip is simple and convenient and has speciality when used for observing the cell flow morphology, can be directly connected with an automatic liquid filler, can be applied to a high intelligence microscope examination medical instrument, can detect cells with different sizes simultaneously, and can improve the observation detecting efficiency.
Description
Technical field
The present invention relates to a kind of novel cell observation chip, from use, is a kind of cell flow morphologic observation chip and the using method of a kind of medical microscope observation.
Background technology
Recently along with medical skill is in the research of hematologic disease and breakthrough, people, to the research of the high intelligence of blood test, the Medical Devices of high automation and production application, also come more.People are while daily life level improves, and also more and more pay close attention to health, medicine body is to normalization and popular development, and the amount detection of the blood sample that people's health check-up gathers is more and more higher.In medical therapy, the identification of the fluid phychology of blood cell is also got more and more with research.So, also change with the detection microslide matched thereupon.But at present, be directly design change to be made into special-shaped microslide substantially, but the cost of manufacture of special-shaped microslide is relatively high, adds the popularization cost of Medical Devices of high intelligence, high automation, and the testing cost of medical research and patient.
Therefore, a kind of more professional blood of human body fluid observation microslide is needed, for medical research and medical diagnostic tests.
Summary of the invention
Technical scheme of the present invention is: a kind of cell flow morphologic observation chip, form primarily of observation unit, it is characterized in that: observation unit comprises with lower component: in one end of oblong chips body, be processed with sample holes, sample holes connects the sample introduction main channel on a long limit of parallel core lamellar body.Mainly ensure that sample introduction has one section of passage to ensure flow velocity.Sample introduction main channel end separates 3 outlets, and a middle outlet taps into sample allotment road, and the outlet of 2, both sides connects 2 oblique by-paths of sample introduction; Article 2, the oblique by-path of sample introduction respectively connects 2 sample introduction allotment roads, is parallel to each other with the sample introduction allotment road of centre is equidistant.Regular arrangement, draws and observes passage.Article three, the concordant long limit perpendicular to chip body, the outlet termination in sample introduction allotment road.This is the chip of a kind of pattern of the present invention.Or outlet termination concordant connecting line be 45 ° of angles in the long limit of chip body, export termination be connected with drainage channel.This is the chip of another kind of pattern of the present invention.Article three, 3 of sample introduction allotment road export the long limit side of terminations from chip body, the observation passage one that interface channel width is descending successively, observation passage two and observation passage three.Observe passage one, observe passage two and observe outlet one end of passage three and be connected 3 respectively and go out sample allotment road, the observation passage one on both sides and the sample allotment road that goes out observing passage three pass through the oblique by-path of sample and converge at out sample main channel with the sample allotment road that goes out observing passage two.Go out sample main channel terminal and connect sample outlet hole.More than form an observation unit.
Chip body, according to the quantity of contain 1 ~ 3 observation unit, is divided into 3 Chip scale.Such as: Chip scale one is containing an observation unit, Chip scale two is containing 2 observation units, and Chip scale three is containing 3 observation units.Observing passage one, observing the chip body region at passage two and observation passage three place is observation area, and the scope identifier of observation area has wire frame, indicates focusing target in the side of adjacent each observation passage.The wire frame of mark and focusing target, object is under microscopical small field of view, and the wire frame of mark is conducive to finding observation passage fast, and the focusing target of mark is conducive to microscope and focuses on fast.Or do not identify wire frame in observation area and focused on target, all in protection scope of the present invention.
In technique scheme, the degree of depth of all passages of described chip body is 100 μm, sample introduction allotment road and go out sample allotment road width 100 μm.Be convenient to unified making template, batch production chip.The width of described observation passage one, observation passage two and observation passage three, 3 passages is respectively 50 μm, 30 μm, 10 μm wide, unified long 10mm.According to the cellular morphology size in blood, the nowed forming of simple differentiation and observation of cell.
In technique scheme, the concordant connecting line in outlet termination in described three sample introduction allotment roads is that 45 ° of angles are in the long limit of chip body, the outlet termination being three sample introduction allotment roads is descending according to connected width, shrinks back to sample holes successively in the outlet termination observing passage one, observation passage two and observation passage three.The outlet termination of the observation passage three that width is minimum is nearest from sample holes.3 outlet concordant connecting lines of termination and the long limit angle at 45 ° of chip body.Article three, on the angle of the side each other of outlet termination, sample introduction allotment road, be connected with drainage channel, the long limit angle at 45 ° of drainage channel and chip body, width is 50 μm.The design of drainage channel is to allow in the observation passage that the impenetrable stream of cells of observation passage that width is little is large to accessible width.
In technique scheme, the using method of described a kind of cell flow morphologic observation chip, is characterized in that, operation steps is as follows: chip sample holes and sample outlet hole are connected kapillary, be fixed on by chip on microscopical objective table, adjustment objective table, makes observation area enter in visual field; By microscope according to observing passage one, observing the focusing target of passage two and observation passage three, reserve focal length, preserve the data reserving focal length.The kapillary of sample holes is connected with the syringe needle of automatic sampler, sets flow velocity, start syringe pump, according to after the fluid access observation passage that microscopic examination is arrived, open high-speed camera, or high definition CCD, the cellular morphology that record is observed passage one, observed passage two and observe in passage three.After observation terminates, sample solution changed by charger, and change the outfit deionized water.Inject deionized water, rinse 5 minutes.
The technology of the present invention feature is observation of cell flow morphology, simple and convenient, again have professional.
Compared with existing body fluid cell observation microslide, the present invention has following beneficial effect: (1) is simply easy to make, with low cost.(2) professional stronger, can directly and automatic liquid adding device be used in conjunction, or to use in high-intelligentization microscopic examination Medical Instruments.(3) a slice can detect the cell of different size simultaneously, improves observation detection efficiency.
Accompanying drawing explanation
Fig. 1 is the chip schematic diagram having 2 observation units of the present invention.
Fig. 2 is the chip schematic diagram having 1 observation unit of the present invention.
Fig. 3 is the chip schematic diagram having drainage channel of the present invention.
Fig. 4 is the enlarged diagram having drainage channel of the present invention.
In figure: 1. chip body; 2. sample holes; 3. sample introduction main channel; 4. the oblique by-path of sample introduction; 5. sample introduction allotment road; 6. observe passage one; 7. observe passage two; 8. observe passage three; 9. go out sample allotment road; 10. go out the oblique by-path of sample; 11. go out sample main channel; 12. sample outlet holes; 13. observation units; 14. observation areas; 15. focus on target; 16. drainage channels.
Embodiment
Further the present invention is illustrated below in conjunction with drawings and Examples.
Embodiment one
With reference to the shape and structure of Fig. 1, a kind of cell flow morphologic observation chip, Chip scale two, has 2 observation units 13, and the degree of depth of all passages of chip body 1 is 100 μm; The width observing passage 1, observation passage 27 and observation passage 38,3 passages is respectively 50 μm, 30 μm, 10 μm wide, unified long 10mm; Sample introduction allotment road 5 and go out sample allotment road 9 width 100 μm.
Operation steps is as follows: chip sample holes 2 and sample outlet hole 12 are connected kapillary, is fixed on by chip on microscopical objective table, and adjustment objective table, makes observation area 14 enter in visual field.By microscope according to observing passage 1, observing the focusing target 15 of passage 27 and observation passage 38, poly-good focal length, strictly according to the facts auto-focusing microscope, preserve the data reserving focal length.The kapillary of sample holes 2 is connected with the syringe needle of automatic sampler, set flow velocity, start syringe pump, according to after the fluid access observation passage that microscopic examination is arrived, open high-speed camera, or high definition CCD, the cellular morphology that record is observed passage 1, observed passage 27 and observe in passage 38, carries out detect delay.After observation terminates, the sample solution changed in automatic sampler is deionized water.Inject deionized water, rinse 5 minutes.
Embodiment two
With reference to the shape and structure of Fig. 2, a kind of cell flow morphologic observation chip, Chip scale one, has 1 observation unit 13, and the degree of depth of all passages of chip body 1 is 100 μm; The width observing passage 1, observation passage 27 and observation passage 38,3 passages is respectively 50 μm, 35 μm, 20 μm wide, unified long 10mm; Sample introduction allotment road 5 and go out sample allotment road 9 width 100 μm.
Operation is with reference to embodiment one.
Embodiment three
With reference to the shape and structure in Fig. 3 and Fig. 4, a kind of cell flow morphologic observation chip, Chip scale one, has 1 observation unit 13, and the degree of depth of all passages of chip body 1 is 100 μm; The width observing passage 1, observation passage 27 and observation passage 38,3 passages is respectively 20 μm, 10 μm, 5 μm wide, unified long 10mm; Sample introduction allotment road 5 and go out sample allotment road 9 width 100 μm.The long limit angle at 45 ° of drainage channel 16 and chip body 1, width is 50 μm.
Operation is with reference to embodiment one.In sample, observe and be greater than 5 μm before passage three, be less than the cell of 10 μm, will enter from drainage channel 16 and observe passage 1 or observe passage 27.Observe and be greater than 10 μm before passage two, be less than the cell of 20 μm, will enter from drainage channel 16 and observe passage 1.
Claims (4)
1. a cell flow morphologic observation chip, form primarily of observation unit (13), it is characterized in that: observation unit (13) comprises with lower component: in one end of oblong chips body (1), be processed with sample holes (2), sample holes (2) connects the sample introduction main channel (3) on a long limit of parallel core lamellar body (1); Sample introduction main channel (3) end separates 3 outlets, and a middle outlet taps into sample allotment road (5), and the outlet of 2, both sides connects 2 oblique by-paths of sample introduction (4); Article 2, the oblique by-path of sample introduction (4) respectively connects 2 sample introduction allotment roads (5), and 2 the sample introduction allotment roads (5) connect are parallel to each other with middle sample introduction allotment road (5) is equidistant; Article three, the concordant long limit perpendicular to chip body (1), the outlet termination of sample introduction allotment road (5), or outlet termination concordant connecting line be 45 ° of angles in the long limit of chip body (1), export termination be connected with drainage channel (16); Article three, sample introduction allotment road (5) 3 export the long limit side of terminations from chip body (1), the observation passage one (6) that interface channel width is descending successively, observation passage two (7) and observation passage three (8); Observe passage one (6), observation passage two (7) is connected 3 respectively with the outlet one end observing passage three (8) and goes out sample allotment road (9), the observation passage one (6) on both sides and the sample allotment road (9) that goes out passing through the oblique by-path of sample (10) and observe passage two (7) in sample allotment road (9) that goes out of observation passage three (8) converge at out sample main channel (11); Go out sample main channel (11) terminal and connect sample outlet hole (12); More than form an observation unit (13); Chip body (1), according to the quantity of 1 ~ 3 observation unit (13) contained, is divided into 3 Chip scale; Observing passage one (6), observing chip body (1) region at passage two (7) and observation passage three (8) place is observation area (14), and the scope identifier of observation area (14) has wire frame, indicates focus on target (15) in the side of adjacent each observation passage; Or do not identify wire frame in observation area (14) and focus on target (15).
2. according to claim 1 cell flow morphologic observation chip, it is characterized in that: the degree of depth of all passages of described chip body (1) is 100 μm; Described observation passage one (6), observation passage two (7) and observation passage three (8), the width of 3 passages is respectively 50 μm, 30 μm, 10 μm wide, unified long 10mm; Sample introduction allotment road (5) and go out sample allotment road (9) width 100 μm.
3. a kind of cell flow morphologic observation chip according to claim 1, it is characterized in that: the concordant connecting line of the outlet termination of described three sample introduction allotment roads (5) is that 45 ° of angles are in the long limit of chip body (1), the outlet termination being three sample introduction allotment roads (5) is descending according to connected width, shrink back to sample holes (2) successively in the outlet termination observing passage one (6), observation passage two (7) and observation passage three (8), the outlet termination of the observation passage three (8) that width is minimum is nearest from sample holes (2); 3 outlet connecting lines of termination and the long limit angle at 45 ° of chip body (1); Article three, sample introduction allotment road (5) export on the angle of the side each other of termination, are connected with drainage channel (16), and the long limit angle at 45 ° of drainage channel (16) and chip body (1), width is 50 μm.
4. the using method of a kind of cell flow morphologic observation chip according to claim 1-3, it is characterized in that, operation steps is as follows:
Chip sample holes (2) and sample outlet hole (12) are connected kapillary, is fixed on by chip on microscopical objective table, adjustment objective table, makes observation area (14) enter in visual field; By microscope according to observing passage one (6), observing the focusing target (15) of passage two (7) and observation passage three (8), reserve focal length, preserve the data reserving focal length; The kapillary of sample holes (2) is connected with the syringe needle of automatic sampler, set flow velocity, start syringe pump, according to after the fluid access observation passage that microscopic examination is arrived, open high-speed camera, or high definition CCD, the cellular morphology that record is observed passage one (6), observed passage two (7) and observe in passage three (8); After observation terminates, sample solution changed by charger, and change the outfit deionized water, injects deionized water rinsing 5 minutes.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510027343.1A CN104597228B (en) | 2015-01-20 | 2015-01-20 | A kind of cell flow morphologic observation chip and using method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510027343.1A CN104597228B (en) | 2015-01-20 | 2015-01-20 | A kind of cell flow morphologic observation chip and using method |
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| CN104597228B CN104597228B (en) | 2016-06-15 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106732839A (en) * | 2016-12-30 | 2017-05-31 | 天津禄浩科技股份有限公司 | A kind of cellular fat particle detections chip and its detection reagent |
| CN109507413A (en) * | 2018-12-13 | 2019-03-22 | 浙江峻山生物科技有限公司 | A kind of immune labeled slide turntable placement rack |
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| US8355876B2 (en) * | 2006-03-31 | 2013-01-15 | Cfd Research Corporation | Microfluidic assay for selection and optimization of drug delivery vehicles to tumors |
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| US20070105206A1 (en) * | 2005-10-19 | 2007-05-10 | Chang Lu | Fluidic device |
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