CN101013069A - Small-amount free cell or tiny sampler collector - Google Patents
Small-amount free cell or tiny sampler collector Download PDFInfo
- Publication number
- CN101013069A CN101013069A CN 200710063773 CN200710063773A CN101013069A CN 101013069 A CN101013069 A CN 101013069A CN 200710063773 CN200710063773 CN 200710063773 CN 200710063773 A CN200710063773 A CN 200710063773A CN 101013069 A CN101013069 A CN 101013069A
- Authority
- CN
- China
- Prior art keywords
- cavity
- sample
- small
- free cell
- collector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a small amount of free cells or small samples collector belonging to the TEM sample production technology area. The collector includes a conical collection cavity, and the cavity of the conical collection cavity is divided into upper and lower parts; a shell; the cavity of the conical collection cavity is placed and fixed within the shell. The upper cavity of the collection cavity makes up of the rigid material, for holding the samples suspension; the lower cavity is made of the gel, used for the sample enrichment. Transparent hard shell is used for inclusive collection cavity, and bearing the centrifugal force of the collection cavity and the sample suspension. Using the collector, it can make the centrifugal sedimentation samples in the gel cavity directly buried, effective solving the problem that a small amount of samples are difficult to collect and pick up, lowering the requirements of the TEM sample preparation to the initial sample volume, and only needing a 1 X 103 yeast, it can produce cell block visible to the naked eye in the gel buried block, and convenient for the repair, cutting, and other operations, and it provides a new sample collection method for electron sample preparation.
Description
Technical field
The invention belongs to transmission electron microscope sample manufacturing technology category, particularly a kind of gatherer that is used to collect small-amount free cell in the suspension or tiny sampler (as subcellular structure etc.).
Background technology
In biological study, obtain a limited number of samples through regular meeting, therefore the value of this sample promotes, even very precious, in case waste then cause considerable damage.But for free cell or tiny sampler (as subcellular structure etc.), the making of its transmission electron microscope sample needs more sample size usually.Such as, the cell initial number that making microorganism Electronic Speculum sample needs usually will be 1 * 10
7More than, minimumly also can not be less than 1 * 10
5This is because in the process that the Electronic Speculum sample is made, can produce the loss on the sample size usually; Secondly, if sample size is very few, the formed sample agglomerate in centrifugal back is then very little, is unfavorable for picking up of sample; Once more, even if can pick up sample sometimes, but through fixing, rinsing, dewater, soak into etc. after the multiple processing procedure, sample is difficult to identification in the resin embedding piece, give to repair block operations and can't carry out.Rashly make the transmission electron microscope sample of small amounts of cells or tiny sampler with conventional method, tend to because sample be difficult to pick up after being difficult to collect, collecting or pick up after be difficult to reason such as section and cause test failure, cause irremediable consequence.
Have the limitation of electron microscopic sample collection method now, limited collection, sample preparation and the electron microscopic observation of small-amount free cell or tiny sampler, fettered carrying out of related scientific research work.
Summary of the invention
The object of the present invention is to provide a kind of be used to collect small-amount free cell or tiny sampler collector, application of the present invention will significantly reduce the requirement of electron microscopyc sample preparation process for cells in sample or tiny sampler initial number.
Small-amount free cell of the present invention or tiny sampler collector is characterized in that, this gatherer comprises a cone-shaped collection chamber, and the cavity in this cone-shaped collection chamber is divided into two sections; A shell; The cavity in cone-shaped collection chamber places and fixes in the enclosure; The epimere cavity of collecting chamber is made of the smooth hard material, can bear stronger centrifugation power, is used for the splendid attire sample suspension; The hypomere cavity is made of gel, and the vertex of a cone radius-of-curvature of hypomere cavity is no more than 20 μ m, and cone angle is no more than 15 °, is beneficial to the sedimentation, slippage of sample and concentrates, and is used for sample enrichment and the sample gel envelope after agglomerating and buries.The shell of gatherer adopts rigid transparent material to constitute, and is used for containing, supporting collection chamber, bears the centrifugation power of collection chamber and sample suspension.
Beneficial effect of the present invention is
1. collection chamber adopts smooth hard material and gel segmentation to constitute, the epimere collecting chamber of inner wall smooth can alleviate the adhesive tape to sample when centrifugal, the hypomere collecting chamber of small-angle helps sedimentation, the slippage of sample and concentrates, thereby has significantly reduced the sample loss in the sample collection process.
2. utilize gel to make up collecting chamber hypomere cavity, can realize the envelope on the spot of sample bolus in the gel awl nest is buried, solved samples such as cell effectively and picked up difficult problem.
3. utilize gatherer of the present invention, can significantly reduce of the requirement of electron microscopyc sample preparation process sample size.Only need 1 * 10
3Individual brewing yeast cell can be made the Electronic Speculum sample smoothly.
4. social agency of the present invention: the invention is collected a kind of new method that provides of making for the electron microscopic sample of a small amount of biological sample, expanded the usable range of transmission electron microscope in the biological study field, limitless effect has been arranged for the development of the research that advances science, expansion people's cognitive domain.
Description of drawings
Fig. 1 is the structural representation of small amounts of cells or tiny sampler collector.
Embodiment
The invention provides a kind of gatherer that is used to collect small-amount free cell or tiny sampler, structural representation at small amounts of cells shown in Figure 1 or tiny sampler collector, comprise a cone-shaped collection chamber, this cone-shaped collection chamber is divided into 2 two sections of epimere collecting chamber 1 and hypomere collecting chambers, the cavity 4 of epimere collecting chamber 1 is made of smooth hard material (plastics, resin or glass), and the cavity 5 of hypomere collecting chamber 2 is made of gel (agarose, agar, gelatin or polyacrylamide); A shell 3 is made of rigid transparent material (plastics, resin).The cavity in cone-shaped collection chamber places and is fixed in the shell 3.Epimere collecting chamber cavity 4 can bear stronger centrifugation power, is used for the splendid attire sample suspension; The vertex of a cone radius-of-curvature of hypomere collecting chamber cavity 5 is no more than 20 μ m, and cone angle is no more than 15 °, is beneficial to the sedimentation, slippage of sample and concentrates, and is used for sample enrichment and the sample gel envelope after agglomerating and buries.The transparent hard shell 3 of gatherer is used for containing, supporting collection chamber, bears the centrifugation power of collection chamber and sample suspension.
The collection process that described small-amount free cell or tiny sampler collector are used for tiny sampler is: centrifugal concentrating behind the filling quantity of sample suspension in the collecting chamber, after treating that sample enrichment in the hypomere cavity of collecting chamber is agglomerating, the sucking-off supernatant, utilizing solidifiable colloidal sol that the sample in the collecting chamber is sealed on the spot buries, treat that colloidal sol solidifies and fixed for after the one with collecting chamber cavity gel, sample is together taken out together with its embedding gel, can fix, processing such as rinsing.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
1. get the cavity of the first Zhi Zuowei epimere of the blue liquid-transfering gun of 1000 μ L collecting chamber, get nickel plating draw point (8 ° of cone angles, needle point radius-of-curvature 20 μ m, bar footpath φ 1.0mm) hole making device as the hypomere collecting chamber, draw point is inserted rifle head inside, and needle point is by leaning out 3mm in the rifle head tip hole and rifle head tip hole being sealed (being used for the making of hypomere collecting chamber).
2. get the first Zhi Zuowei shell of the yellow liquid-transfering gun of 200 μ L, sealing by fusing rifle head point to the low-freezing agarose 2% redistilled water solution (35 ℃) that wherein injects 200 μ L, makes agarose (being used for the preparation of hypomere collecting chamber cavity) be full of whole rifle cephalic par.
3. insert in the yellow rifle head with the blue rifle head that is about to be inserted with draw point, make two rifle headgears close sealing.
4. treat to extract draw point after agarose solidifies, the Ago-Gel after solidifying is the cavity of hypomere collecting chamber, and the awl nest aperture that stays in gel after draw point is pulled out is the hypomere collecting chamber.
5. in blue rifle head (epimere collecting chamber), inject 1mL the saccharomycete suspension (cell concentration be 1 * 103/mL), will take out behind the centrifugal 5min of gatherer 200g.
6. shift out supernatant, pull out blue rifle head, injection an amount of agarose (35 ℃) and in gel awl nest (hypomere collecting chamber) with the centrifugal 5min of 200g, condensation agarose.
7. yellow rifle head (shell) is cut off, take out the gel piece that contains cenobium, resulting sample blocks can be directly used in fixing the processing.
Claims (8)
1. small-amount free cell or tiny sampler collector is characterized in that this gatherer comprises a cone-shaped collection chamber, and the cavity in this cone-shaped collection chamber is divided into two sections; A shell; The cavity in cone-shaped collection chamber places and fixes in the enclosure.
2. according to described small-amount free cell of claim 1 or tiny sampler collector, it is characterized in that described epimere cavity is made of the smooth hard material, is used for the splendid attire sample suspension, and can bear stronger centrifugation power.
3. according to described small-amount free cell of claim 1 or tiny sampler collector, it is characterized in that described hypomere cavity is made of gel, is used for sample enrichment.
4. according to claim 1 or 3 described small-amount free cell or tiny sampler collectors, it is characterized in that the radius-of-curvature of the described hypomere cavity vertex of a cone is not more than 20 μ m, cone angle is no more than 15 °.
5. according to small-amount free cell described in the claim 1 or tiny sampler collector, it is characterized in that described shell adopts rigid transparent material to constitute, and is used to contain collecting chamber, supports the collecting chamber cavity, bears the centrifugation power of collecting chamber cavity and sample suspension.
6. according to small-amount free cell described in the claim 1 or tiny sampler collector, it is characterized in that the smooth hard material of described formation epimere cavity is plastics, resin or glass.
7. according to small-amount free cell described in the claim 1 or tiny sampler collector, it is characterized in that the gel of described formation hypomere cavity is agarose, agar, gelatin or polyacrylamide.
8. according to small-amount free cell described in the claim 1 or tiny sampler collector, it is characterized in that the rigid transparent material of described structure shell is plastics or resin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100637734A CN101013069B (en) | 2007-02-09 | 2007-02-09 | Small-amount free cell or tiny sampler collector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100637734A CN101013069B (en) | 2007-02-09 | 2007-02-09 | Small-amount free cell or tiny sampler collector |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101013069A true CN101013069A (en) | 2007-08-08 |
| CN101013069B CN101013069B (en) | 2010-09-08 |
Family
ID=38700724
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007100637734A Expired - Fee Related CN101013069B (en) | 2007-02-09 | 2007-02-09 | Small-amount free cell or tiny sampler collector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101013069B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102378641A (en) * | 2009-03-31 | 2012-03-14 | 吉纳瓦克有限公司 | Method and sample holding assembly for use in sample preparation |
| CN102847567A (en) * | 2012-08-24 | 2013-01-02 | 浙江硕华医用塑料有限公司 | Microscale sampling rod |
| CN104359746A (en) * | 2014-11-19 | 2015-02-18 | 白银市第一人民医院 | Cell mass collector and method for collecting cells in liquid specimen |
| CN113462518A (en) * | 2021-07-06 | 2021-10-01 | 中国人民解放军军事科学院军事医学研究院 | Method for extracting trace amount of fresh peripheral blood nucleated cell protein and special device thereof |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US588831A (en) * | 1897-08-24 | lanhoffer | ||
| US4528187A (en) * | 1982-03-12 | 1985-07-09 | Truglio William J | Apparatus for collecting and microscopically examining a specimen |
| FR2855831B1 (en) * | 2003-06-04 | 2005-09-02 | Bertin Technologies Sa | DEVICE FOR COLLECTING PARTICLES AND MICROORGANISMS IN AMBIENT AIR |
| CN2892868Y (en) * | 2005-12-06 | 2007-04-25 | 刘瑞东 | Cell detaching tube |
-
2007
- 2007-02-09 CN CN2007100637734A patent/CN101013069B/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102378641A (en) * | 2009-03-31 | 2012-03-14 | 吉纳瓦克有限公司 | Method and sample holding assembly for use in sample preparation |
| CN102378641B (en) * | 2009-03-31 | 2017-03-29 | 吉纳瓦克有限公司 | Component is kept for the method in sample preparation and sample |
| CN102847567A (en) * | 2012-08-24 | 2013-01-02 | 浙江硕华医用塑料有限公司 | Microscale sampling rod |
| CN104359746A (en) * | 2014-11-19 | 2015-02-18 | 白银市第一人民医院 | Cell mass collector and method for collecting cells in liquid specimen |
| CN113462518A (en) * | 2021-07-06 | 2021-10-01 | 中国人民解放军军事科学院军事医学研究院 | Method for extracting trace amount of fresh peripheral blood nucleated cell protein and special device thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101013069B (en) | 2010-09-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101013069B (en) | Small-amount free cell or tiny sampler collector | |
| CN102818723B (en) | Method of electrolytically extracting and detecting fine inclusions in steel | |
| CN105842312A (en) | Nano flower-shaped ultramicro gold electrode and preparation and application thereof | |
| Nishihara et al. | Cloning and characterization of endosymbiotic algae isolated from Paramecium bursaria | |
| CN104877928A (en) | Mixed bacteria producing biosurfactant and screening method of mixed bacteria | |
| CN201034852Y (en) | Deposited matter interstitial water quick-speed capturing machine | |
| CN107505176A (en) | Method for extracting and separating nano-scale precipitate in steel by adopting organic electrolyte | |
| CN103163162A (en) | Method for identifying nanogold in tetrahymena thermophila in situ by transmission electron microscope and energy spectrum | |
| Su et al. | [Retracted] Application of Polypyrrole Cellulose Nanocrystalline Composite Conductive Material in Garment Design | |
| CN208735488U (en) | A kind of household emergency lighting system based on plant illumination power generation | |
| CN110467180A (en) | A kind of preparation method of the biomass derived Carbon Materials for sodium-ion battery | |
| TWI725439B (en) | Microbial fuel cell device and method for immobilizing algae strain | |
| CN207476707U (en) | A kind of plant nematode picking tool | |
| CN103436430A (en) | Algae culture apparatus | |
| CN201149737Y (en) | Seashell larva integral specimen prepared by concave slide | |
| CN202105900U (en) | Forming cutter for punched hole of liquid collecting pipe | |
| CN207600799U (en) | A kind of Diatoms In Surface Sediments of Central sampler | |
| CN203174088U (en) | Single cell separator | |
| CN202351165U (en) | Sample bearing mesh box for transmission electron microscope | |
| CN103571755B (en) | A kind of acquisition of chain band algae NMX451 and the method for genetic transformation | |
| CN109256528B (en) | Lithium iron phosphate-bacterial cellulose-graphene composite material and preparation method and application thereof | |
| CN106495142B (en) | By the method for furfural resin preparation class grapheme material, class grapheme material and application thereof | |
| CN207151303U (en) | One kind plantation tree peony flowerpot | |
| CN105567570A (en) | Production method for Cordyceps sobolifera mycelium and exopolysaccharide liquid fermentation | |
| CN206725317U (en) | A kind of centrifuge tube prepared for cell block |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100908 Termination date: 20130209 |