CN207476707U - A kind of plant nematode picking tool - Google Patents
A kind of plant nematode picking tool Download PDFInfo
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- CN207476707U CN207476707U CN201721547989.3U CN201721547989U CN207476707U CN 207476707 U CN207476707 U CN 207476707U CN 201721547989 U CN201721547989 U CN 201721547989U CN 207476707 U CN207476707 U CN 207476707U
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- nematode
- capillary
- connecting tube
- picking
- diaphragm seal
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Abstract
The utility model discloses a kind of plant nematode picking tool, including rubber head, connecting tube and capillary, one end of the connecting tube is connect with rubber head, the other end is connect with capillary, the junction of the connecting tube and rubber head is provided with the first diaphragm seal, and the junction of the connecting tube and capillary is provided with the second diaphragm seal.The plant nematode picking tool of the utility model operates convenient for grasping, and capillary is smooth, transparent, under microscope or stereoscope can clear view to the nematode present position of institute's picking, the phenomenon that there is no adhering to, the loss of nematode will not be caused.Once can just be shifted with picking single nematode, can also a plurality of nematode of picking retransfer, transfer effect is good, and speed is fast, efficient, the picking particularly suitable for tiny line style nematode.
Description
Technical field
The utility model belongs to the transfer tool technical field of nematode, and in particular to a kind of plant nematode picking work
Tool, particularly for the transfer picking of Bursaphelenchus nematodes.
Background technology
Bursaphelenchus nematodes (Bursaphelenchus) are a kind of plant nematode propagated by coleopteron or food
Bacterium nematode, since 1971, due to causing the Bursaphelenchus xylophilus (Bursaphelenchus of Japanese a large amount of pine deaths
Xylophilus the caused seriousness endangered of pathogenic confirmation and the nematode), much country is classified as in the world
Important quarantine harmful organisms, Bursaphelenchus nematodes also cause the extensive concern of nematode educational circles and plant quarantine department.Especially
It is from after Portugal in 1998 finds Bursaphelenchus xylophilus, and Bursaphelenchus nematodes cause the great attention of EU countries, to the category
Nematode is taken a broad survey, and by the detection to a large amount of pine samples, a large amount of novel species are found.So far, the umbrella described is slided
Sword category nematode is more than 100 kinds.Either nematode in pine body is identified or is collected the pine line from countries in the world
The strain of worm worm establishes nematode gene resources bank in pine body or carries out in pine body the diversity of nematode or quarantine departments to intercepting and capturing
Wood packing box etc. quarantine, the separated nematode arrived of picking is required to, especially for carrying out every research, example in a deep going way
Such as retrospect of Bursaphelenchus xylophilus Population variation law study, epidemic disease source, inbred strais structure, single nematode DNA extractions, it is often necessary to
Accurate picking single nematode.
Bursaphelenchus nematodes polypide is thinner, and general body grows 0.4~1.5mm, and 30 μm or so of diameter, body is relatively sliding, there is certain bullet
Property.Picking plant nematode is typically to choose needle using No. 0 insect needle making at this stage, since insect needle is relatively large in diameter, 300
μm or so, using insect needle make when choosing needle transfer nematode, accidentally with other vessel, such as container edge gently percussion,
It is easily lost nematode.Flat platinum wire is struck in some laboratories using head end, but there is the deficiency similar with insect needle with platinum wire.
Also some scholars carry out nematode picking with the pipette tips of 0.5 μ l, disadvantage is that nematode is easy to be adhered in plastics pipette tips,
Equally it is easy to cause nematode loss.In the case of extremely lacking for the quantity being separated in experimentation or quarantine procedures, cause
Unpredictable loss.Therefore, it is badly in need of finding a kind of tool of efficiently and accurately picking nematode to meet the needs of production or scientific research.
Utility model content
Purpose of utility model：In view of the deficienciess of the prior art, the purpose of this utility model be to provide it is a kind of it is easy to operate,
Transfer effect is good, fireballing plant nematode picking tool.
Technical solution：In order to realize above-mentioned purpose of utility model, the technical solution adopted in the utility model is as follows：
A kind of plant nematode picking tool, including rubber head, connecting tube and capillary, one end of the connecting tube and glue
Head connection, the other end are connect with capillary, and the junction of the connecting tube and rubber head is provided with the first diaphragm seal, the connecting tube
The junction of capillary is provided with the second diaphragm seal.
Preferably, the connecting tube is plastic conduit or glass tube.
Preferably, the connecting tube is moccasin pipe, be set with pipette tips outside the moccasin pipe, the butt end of the pipette tips with
Rubber head connects, and the junction of the pipette tips and rubber head is provided with third diaphragm seal.
Preferably, the internal diameter of the moccasin pipe is 0.9mm；A diameter of 0.9mm of the capillary, length for 4~
6cm。
Preferably, first diaphragm seal, the second diaphragm seal and third diaphragm seal are Parafilm sealed membranes.
Preferably, second diaphragm seal is wrapped in the outside of capillary, capillary is wound after the second diaphragm seal
One end is inserted into connecting tube.
Preferably, the pipette tips are 1mL pipette tips.
Preferably, the capillary is capillary made of glass.
Advantageous effect：Compared with prior art, the utility model has the following advantages：
The plant nematode picking tool of the utility model, rubber head hold operation convenient for middle finger, forefinger and thumb.For
During plant nematode picking, as long as the target nematode by capillary in nematode suspension, by siphonage, nematode into
Enter in capillary, when the nematode of institute's picking is transferred to other vessel, only need to gently squeeze rubber head, can be squeezed out, it will
Nematode is transferred to any position for wanting to place.Capillary glass tube is smooth, transparent, under microscope or stereoscope can clear view to institute
The nematode present position of picking there is no the phenomenon that adherency, will not cause the loss of nematode.It once can be with picking single nematode just
Transfer, can also a plurality of nematode of picking retransfer, transfer effect is good, and speed is fast, efficient, particularly suitable for tiny line style
The picking of nematode.
Description of the drawings
Fig. 1 is 1 structure diagram of plant nematode picking device embodiment；
Fig. 2 is 2 structure diagram of plant nematode picking device embodiment；
Fig. 3 is Bursaphelenchus xylophilus worm strain SX14-1 single nematode DNA cloning product electrocapillary phoresis figures, from left to right swimming lane：
1.Maker, 2-18 swimming lane are respectively 01-17 primers.
Specific embodiment
In the following with reference to the drawings and specific embodiments, the utility model is furtherd elucidate, embodiment is with the utility model skill
Implemented under premised on art scheme, it should be understood that these embodiments are merely to illustrate the utility model rather than limit this practicality
Novel range.
Embodiment 1
A kind of plant nematode picking tool, including rubber head 1, connecting tube 3 and capillary 4, one end of connecting tube 3 and glue
First 1 connection, the other end connect with capillary 4, and the junction of connecting tube 3 and rubber head 1 is provided with the first diaphragm seal 5, connecting tube 3 and
The junction of capillary 4 is provided with the second diaphragm seal 6.Connecting tube 3 is plastic conduit or glass tube, increases picking tool
Integral strength.
Embodiment 2
As preferred embodiment, as shown in Fig. 2, a kind of plant nematode picking tool, including rubber head 1, pipette tips 2,
Connecting tube 3 and capillary 4, connecting tube 3 are moccasin pipe, and moccasin pipe is easy to connect with capillary 4, and pipette tips 2 are socketed in outside moccasin pipe,
Pipette tips 2 are made of the plastics or glass of hard, and 2 butt end of pipette tips is connect with rubber head 1；Moccasin pipe is stretched into inside pipette tips 4, moccasin pipe
One end connect with rubber head 1, the other end is pierced by from 2 taper end of pipette tips and is connect with capillary 4, the junction of moccasin pipe and rubber head 1
The first diaphragm seal 5 is provided with, capillary 4 and the junction of moccasin pipe are provided with the second diaphragm seal 6, in the company of pipette tips 2 and rubber head 1
The place of connecing is provided with third diaphragm seal 7.Capillary 4 is capillary made of glass.First diaphragm seal 5 is wrapped in moccasin pipe and rubber head
1 junction, the second diaphragm seal 6 are wrapped in the outside of capillary 4, and capillary 4 winds one end insertion after the second diaphragm seal 6
In moccasin pipe.Install one layer of pipette tips 2 additional outside moccasin pipe, operating personnel is held when laboratory uses it is more convenient, it is also more convenient and
The connection of rubber head.
Pipette tips 2 are 1mL pipette tips, and the internal diameter of moccasin pipe is 0.9mm；A diameter of 0.9mm of capillary 4, length 4-6cm,
Preferred length is 5cm.
First diaphragm seal 5, the second diaphragm seal 6 and third diaphragm seal 7 are given birth to for Parafilm sealed membranes or politef
The material strip either film of other good seal performances or the diaphragm seal formed after being solidified using fluid sealant.
The case for now choosing several practical applications verifies the using effect of the utility model：
Application case 1：Using the tool, it is very beneficial for the apparent pine line of accurate picking Sex Differentiation under the microscope
Female, the male larva of worm mates in the 5mm × 5mm Botrytis cinerea fungus blocks being transferred in pairs, and the experiment is by picking from not
With 20 generation of nematode and its filial generation inbreeding of parent, picking nematode more than 5000 is right altogether, obtains a large amount of inbred strais offsprings.It effectively prevents
Liquid-transfering gun is too thick, is unfavorable for the accurate picking of pairs of nematode, it sometimes appear that 3 nematodes (even more) are chosen the feelings into water
Condition.Hybrid experiment (hybridization of such as Bursaphelenchus xylophilus and B. mucronatus) of the tool between different nematode kinds are carried out equally has pole
Its apparent advantage.
Application case 2：The plant nematode picking tool of the utility model is chosen particularly suitable for the accurate of tiny nematode
It takes and safe transfer.Using the plant nematode picking tool of the utility model, will successfully come from U.S.'s wood packing box
1 female 2 male totally 3 Bursaphelenchus xylophiluses only found, are transferred in 5mm × 5mm ash grape fungus blocks, establish population.It effectively prevents
It is shifted using pipette tips, nematode, which is easily adhered to, causes nematode to be lost on plastic rifle head, the feelings so as to cause population foundation failure
Condition.
Application case 3：The plant nematode picking tool of the utility model is particularly suitable for tiny line style nematode
Accurate picking.It using the tool picking single nematode, can not only be lost to avoid nematode, and carrying water is considerably less, is conducive to
Accurate single nematode carries out DNA extractions under the microscope, and water shortage entrained by extraction single nematode is chosen using the pipette tips of 5 μ L
The 1/10 of line taking worm institute water amount is conducive to further extract single nematode DNA using kit, carries out capillary electrophoresis detection
Deng experiment, as shown in figure 3, using picking tool picking Bursaphelenchus xylophilus worm strain SX14-1 single nematodes, after extracting DNA, with 17
Capillary Electrophoresis is carried out to primer, obtains clearly amplified production.
The above is only the preferred embodiment of the utility model, it is noted that for the common skill of the art
For art personnel, under the premise of the utility model principle is not departed from, several improvements and modifications can also be made, these improve and
Retouching also should be regarded as the scope of protection of the utility model.
Claims (8)
1. a kind of plant nematode picking tool, it is characterised in that：Including rubber head (1), connecting tube (3) and capillary (4), institute
The one end for stating connecting tube (3) is connect with rubber head (1), and the other end is connect with capillary (4), the connecting tube (3) and rubber head (1)
Junction is provided with the first diaphragm seal (5), and the connecting tube (3) and the junction of capillary (4) are provided with the second diaphragm seal
(6)。
2. plant nematode picking tool according to claim 1, it is characterised in that：The connecting tube (3) is hard
Plastic tube or glass tube.
3. plant nematode picking tool according to claim 1, it is characterised in that：The connecting tube (3) is moccasin
Pipe, in the moccasin pipe outer cover equipped with pipette tips (2), the butt end of the pipette tips (2) is connect with rubber head (1), in the pipette tips (2)
The junction of rubber head (1) is provided with third diaphragm seal (7).
4. plant nematode picking tool according to claim 3, it is characterised in that：The internal diameter of the moccasin pipe is
0.9mm；A diameter of 0.9mm of the capillary (4), length are 4~6cm.
5. plant nematode picking tool according to claim 3, it is characterised in that：First diaphragm seal (5),
Two diaphragm seals (6) and third diaphragm seal (7) are Parafilm sealed membranes.
6. plant nematode picking tool according to claim 1, it is characterised in that：Second diaphragm seal (6) twines
It is wound on the outside of capillary (4), one end that capillary (4) winds after the second diaphragm seal (6) is inserted into connecting tube (3).
7. plant nematode picking tool according to claim 3, it is characterised in that：The pipette tips (2) are 1mL rifles
Head.
8. the plant nematode picking tool according to claim 1 or 3, it is characterised in that：The capillary (4) is glass
Capillary made of glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721547989.3U CN207476707U (en) | 2017-11-20 | 2017-11-20 | A kind of plant nematode picking tool |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721547989.3U CN207476707U (en) | 2017-11-20 | 2017-11-20 | A kind of plant nematode picking tool |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207476707U true CN207476707U (en) | 2018-06-12 |
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ID=62474121
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721547989.3U Expired - Fee Related CN207476707U (en) | 2017-11-20 | 2017-11-20 | A kind of plant nematode picking tool |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207476707U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110150194A (en) * | 2019-06-06 | 2019-08-23 | 深圳金普迈生物科技有限公司 | A kind of measuring method of microzooplankton swimming rate |
-
2017
- 2017-11-20 CN CN201721547989.3U patent/CN207476707U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110150194A (en) * | 2019-06-06 | 2019-08-23 | 深圳金普迈生物科技有限公司 | A kind of measuring method of microzooplankton swimming rate |
| CN110150194B (en) * | 2019-06-06 | 2021-04-20 | 深圳金普迈生物科技有限公司 | Method for measuring swimming speed of micro zooplankton |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Nanjing biological pest control technology, Limited by Share Ltd Assignor: Nanjing Forestry University Contract record no.: 2018320000400 Denomination of utility model: Plant nematode picking instrument Granted publication date: 20180612 License type: Common License Record date: 20181218 Assignee: Nanjing Forest Protection Bird Biotechnology Co., Ltd. Assignor: Nanjing Forestry University Contract record no.: 2018320000399 Denomination of utility model: Plant nematode picking instrument Granted publication date: 20180612 License type: Common License Record date: 20181218 Assignee: Guizhou Linxin Ecological Agriculture Technology Co., Ltd. Assignor: Nanjing Forestry University Contract record no.: 2018320000401 Denomination of utility model: Plant nematode picking instrument Granted publication date: 20180612 License type: Common License Record date: 20181218 |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180612 Termination date: 20191120 |