CN201049281Y - Small-sized ultrafiltration system for laboratory - Google Patents
Small-sized ultrafiltration system for laboratory Download PDFInfo
- Publication number
- CN201049281Y CN201049281Y CNU2007200938031U CN200720093803U CN201049281Y CN 201049281 Y CN201049281 Y CN 201049281Y CN U2007200938031 U CNU2007200938031 U CN U2007200938031U CN 200720093803 U CN200720093803 U CN 200720093803U CN 201049281 Y CN201049281 Y CN 201049281Y
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- ultrafiltration
- tube
- film
- peristaltic pump
- milipore filter
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Abstract
The utility model relates to a miniature ultrafiltration system for laboratory, comprising three main components which are an ultrafiltration chamber, a miniature ultrafiltration membrane packet and a peristaltic pump, wherein, the ultrafiltration membrane packet is placed inside the ultrafiltration chamber for separating samples and provided with three interfaces which are a liquid inlet, a liquid outlet on membrane, a liquid outlet under membrane; a speed regulating knob regulating the peristaltic pump can regulate the flow speed of samples entering an ultrafiltration system and the magnitude of pressure on magnitude; the flow direction of liquid can be controlled by regulating a pump head of the peristaltic pump to change direction. According to different requirements of experiments, the biochemical samples mixed with molecular weight levels can be graded, concentrated or desalted by selecting the ultrafiltration membrane packets with different molecular weight levels. The utility model can be used for the concentration, separation, purification of proteins, antibodies, single peptides (polypeptides), enzymes, nucleic acids, viruses and polysaccharides, the desalination, dealcoholization and buffer solution exchange of macromolecule biological samples, as well as the heat source removal of small molecular samples. The utility model is low in construction cost, convenient to operate, strong in practicality and easy to process as well as produce.
Description
Technical field
The utility model is the laboratory filter, is specifically related to the small-sized ultrafiltration system in a kind of laboratory.
Background technology
Ultrafiltration: being called for short UF, is to be motive force with pressure, the material screening process that utilizes the different apertures of milipore filter that liquid is separated.Usually adopt the slipstream (TFF) and the stream technology of circling round, to reduce the absorption of milipore filter to sample.
The ultrafiltration apparatus of current laboratory or industrial employing mostly is the product of U.S. Pall company or U.S. Millipore company greatly, and the product cost is higher, and homemade ultrafiltration apparatus is second-rate, designs unreasonablely, is difficult to satisfy the requirement of modern laboratory technique.Occupy this kind present situation, be necessary to design a kind of cheap, rational in infrastructure, can satisfy the device of laboratory small lot ultrafiltration experiment.
Summary of the invention
The utility model purpose is in order to address the above problem, provide a kind of have cheap, built-in milipore filter bag, easy and simple to handle, practical, be easy to the small-sized ultrafiltration system in laboratory of processing.
The utility model is laboratory small lot ultrafiltration sample (liquid) experimental provisions such as biology, chemistry, medicine and pharmacology, and this device comprises annexes such as ultrafiltration chamber, small-sized milipore filter bag, three primary clusterings of peristaltic pump and silicone tube.Peristaltic pump links to each other with ultrafiltration chamber feed tube external tapping by peristaltic pump tube, and the milipore filter bag is used for sample separation, is provided with three interfaces: an inlet, a film upper liquid outlet, a film lower liquid outlet.The milipore filter bag is purchased from market.Milipore filter bag inlet links to each other with ultrafiltration chamber feed tube inner joint by silicone tube, and milipore filter coating upper liquid outlet links to each other with recycle control valve student interface on the indoor film of ultrafiltration, and the film lower liquid outlet is derived by right side, ultrafiltration chamber cinclides by silicone tube.The ultrafiltration chamber is a rectangle stainless steel device, built-in milipore filter bag, front panel is with backflow controlled value, three-way control valve door, feed tube interface on Pressure gauge (hydraulic pressure), the film, and sidewall (as right side wall) is with backflow interface tube and the dirty fluid hole of film on two films.The ultrafiltration chamber is used for fixing the milipore filter bag and makes it built-in, and appended Pressure gauge is used to detect film upward pressure (inlet hydraulic), and backflow controlled value is used for the flow and the film upward pressure of phegma on the controlling diaphragm on the film, and the three-way control valve door is used to control the flow direction of phegma.The feed tube external tapping links to each other with the pump head of peristaltic pump by peristaltic pump tube, Pressure gauge links to each other with the feed tube inner joint, the feed tube inner joint links to each other with milipore filter bag inlet by silicone tube, on the film on the backflow controlled value interface link to each other by the side interface of silicone tube with the three-way control valve door, the last lower interface of three-way control valve door links to each other by backflow interface tube A, B on two films in silicone tube and the ultrafiltration chamber right side wall.Peristaltic pump is the power set that sample enters the milipore filter bag, the ultrafiltration sample can be pumped into the milipore filter bag by peristaltic pump tube from the container of adorning sample.Regulate the speed governing knob of peristaltic pump and can regulate the size that sample enters the flow velocity and the film upward pressure of ultrafiltration system.The pump head of regulating peristaltic pump turns to the flow direction that can control liquid.Other milipore filter bag of different molecular weight level is selected in experimental different requirement, can carry out classification, concentrate or desalination etc. being mixed with other biological sample of different molecular weight level (protein, antibody, polypeptide, enzyme, polysaccharide etc.).Can be used for that protein, antibody, list (many) peptide, enzyme, nucleic acid and virus, polysaccharide etc. concentrate, isolation and purification and big molecular biosciences sample desalination, dealcoholysis, buffer-exchanged, and little minute subsample remove thermal source.
The utlity model has cheap, built-in milipore filter bag, easy and simple to handle, practical, be easy to processing.
Description of drawings
The utility model is described in further detail below in conjunction with the drawings and specific embodiments.
Fig. 1 for the laboratory with small-sized ultrafiltration system structural representation.
Fig. 2 is the ultrafiltration chamber interior structural representation of laboratory with small-sized ultrafiltration system.
Among Fig. 1, Fig. 2,1-feed tube external tapping; Backflow controlled value on the 2-film; The 3-Pressure gauge; 4-three-way control valve door; 5-peristaltic pump rotational speed regulation knob; 6-peristaltic pump power switch; The 7-peristaltic pump turns to adjusting knob; 8-peristaltic pump pump head; 9-ultrafiltration chamber feed tube; 10-peristaltic pump feed tube; Return duct B on the 11-film; The dirty drain pipe of 12-film; The 13-sample is contained the liquid device; The 14-film washing liquid is contained the liquid device; The dirty fluid of 15-film is contained the liquid device; The last interface of 16-three-way control valve door; The lower interface of 17-three-way control valve door; The side interface of 18-three-way control valve door; 19-milipore filter bag; The 20-Pressure gauge; 21-three-way control valve door; Recycle control valve student interface on the 22-film; Interface on the backflow controlled value on the 23-film; Backflow interface tube B on the 24-film; Backflow interface tube A on the 25-film; The dirty fluid hole of 26-film; 27-film upper liquid outlet; 28-milipore filter bag inlet; 29-film lower liquid outlet; 30-feed tube inner joint; I-ultrafiltration chamber; The II-peristaltic pump
The specific embodiment
This device comprises annexes such as ultrafiltration chamber, small-sized milipore filter bag, three primary clusterings of peristaltic pump and silicone tube, and small-sized milipore filter bag and peristaltic pump can be purchased from the market, and silicone tube requires good toughness and acid and alkali-resistance, and ultrafiltration chamber feed tube will be used peristaltic pump tube.
I inside, ultrafiltration chamber, Pressure gauge 3 is fixed by Pressure gauge and is fixed on ultrafiltration chamber I with feed tube inner joint connection assembly 20 and upward and with feed tube inner joint 30 links to each other, on the film on the backflow controlled value interface 23 link to each other with the side interface 18 of three-way control valve door by silicone tube, the last interface 16 of three-way control valve door links to each other with backflow interface tube 25 on the right side wall film of ultrafiltration chamber by silicone tube, and the lower interface 17 of three-way control valve door links to each other with backflow interface tube 24 on the right side wall film of ultrafiltration chamber by silicone tube.During ultrafiltration milipore filter bag 19 is put in the I of ultrafiltration chamber, milipore filter bag inlet 28 links to each other with ultrafiltration chamber feed tube inner joint 30 by silicone tube, milipore filter coating upper liquid outlet 27 links to each other by recycle control valve student interface 22 on the indoor film of silicone tube and ultrafiltration, and film lower liquid outlet 29 is derived by the dirty fluid hole 26 of ultrafiltration chamber right side wall film by silicone tube.
Ultrafiltration chamber I is put peristaltic pump II pump housing top, peristaltic pump tube is packed in the peristaltic pump pump head, top links to each other with feed tube external tapping 1, be called ultrafiltration chamber feed tube 9, the bottom is that peristaltic pump feed tube 10 is put into sample Sheng liquid device 13 bottoms, return duct 11-holds on the external tapping of backflow interface tube 24 on the junctional membrane on the film, and the other end is put into film washing liquid and contained liquid device 14, puts into the dirty fluid Sheng of film liquid device 15 by dirty drain pipe 12 mouths of pipe of film that the dirty fluid hole 26 of film is derived.
In containing liquid device 13, add an amount of distilled water; open peristaltic pump power switch 6; backflow controlled value 2 is to full open position on the adjusting film; regulate peristaltic pump rotational speed regulation knob 5 distilled water is pumped into protection liquid or the alkali lye in the flush away milipore filter bag in the indoor milipore filter bag of ultrafiltration with suitable flow velocity; backflow controlled value 2 is to semi-open state and regulate peristaltic pump rotational speed regulation knob 5 on the intermediate regulations film; make that return duct II and the dirty drain pipe 12 of film have liquid to flow out on the film; and control pressure well by Pressure gauge 3 and make it to meet the pressure requirement of milipore filter bag; to wash out after washing that waste liquid is outwelled and clean all with distilled water and contain liquid devices; to treat that the ultrafiltration sample adds in the Sheng liquid device 13; return duct on the film 11 is contained taking-up in the liquid device 14 from film washing liquid; insert and contain in the liquid device 13; backflow controlled value 2 is to full open position on the adjusting film; regulating peristaltic pump rotational speed regulation knob 5 will treat in the interior milipore filter bag of ultrafiltration sample pump excess of imports filter chamber; behind the interior air of emptying milipore filter bag; regulate on the film backflow controlled value 2 to semi-open state and regulate peristaltic pump rotational speed regulation knob 5; make that return duct 11 and the dirty drain pipe 12 of film all have liquid to flow out on the film, and control pressure well and make it to meet the pressure requirement of milipore filter bag.Sample flows in the dirty fluid Sheng of the film liquid device 15 by the dirty drain pipe 12 of film under the film, and the sample that refluxes on the film (not passing through the sample of milipore filter) flows into sample again by return duct on the film 11 and contains in the liquid device 13.Can be after ultrafiltration finishes by regulating three-way control valve door 4 through big molecule concentrate on backflow interface tube 25 reclamation films on the film.After ultrafiltration finishes per sample the different washing lotions of kind different choice wash the residual sample of absorption in the milipore filter bag off, protein-based sample generally washes with the sodium hydrate aqueous solution of 0.1-0.5 molar concentration, washes in the milipore filter packet procedures and can select washing lotion inflow film washing liquid Sheng liquid device 14 or circulation inflow sample to contain in the liquid device 13 by adjusting three-way control valve door.Clean back milipore filter Bao Yaoyong protection liquid (the 0.1 molar concentration left and right sides sodium hydrate aqueous solution or the 0.3 ‰ sodium azide aqueous solution) and seal up for safekeeping, prevent interior minister bacterium and keep hygrometric state.
Claims (2)
1. the small-sized ultrafiltration system in laboratory, it is characterized in that by the ultrafiltration chamber, small-sized milipore filter bag, three primary clusterings of peristaltic pump and silicone tube are formed, peristaltic pump links to each other with ultrafiltration chamber feed tube external tapping by peristaltic pump tube, arrival end connects a container by silicone tube, peristaltic pump is provided with power switch, rotational speed regulation knob and turn to adjusting knob, the indoor milipore filter bag of putting of ultrafiltration, the milipore filter bag is provided with three interfaces: an inlet, a film upper liquid outlet, a film lower liquid outlet, milipore filter bag inlet links to each other with ultrafiltration chamber feed tube inner joint by silicone tube, milipore filter coating upper liquid outlet links to each other with recycle control valve student interface on the indoor film of ultrafiltration, and the film lower liquid outlet is derived by right side, ultrafiltration chamber cinclides by silicone tube; Ultrafiltration chamber front panel is with hydraulic gage, backflow controlled value on the film, the three-way control valve door, the feed tube interface, sidewall is with backflow interface tube and the dirty fluid hole of film on two films, the feed tube external tapping links to each other with the pump head of peristaltic pump by peristaltic pump tube, Pressure gauge links to each other with the feed tube inner joint, the feed tube inner joint links to each other with milipore filter bag inlet by silicone tube, on the film on the backflow controlled value interface link to each other by the side interface of silicone tube with the three-way control valve door, the last lower interface of three-way control valve door by silicone tube respectively with ultrafiltration chamber right side wall on two films the backflow interface tube link to each other the terminal container that connects of return duct.
2. by the small-sized ultrafiltration system in laboratory of claim 1, it is characterized in that the ultrafiltration chamber is a rectangle stainless steel device, silicone tube is a toughness acid and alkali-resistance pipe, and ultrafiltration chamber feed tube is a peristaltic pump tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200938031U CN201049281Y (en) | 2007-05-30 | 2007-05-30 | Small-sized ultrafiltration system for laboratory |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200938031U CN201049281Y (en) | 2007-05-30 | 2007-05-30 | Small-sized ultrafiltration system for laboratory |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201049281Y true CN201049281Y (en) | 2008-04-23 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2007200938031U Expired - Fee Related CN201049281Y (en) | 2007-05-30 | 2007-05-30 | Small-sized ultrafiltration system for laboratory |
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| Country | Link |
|---|---|
| CN (1) | CN201049281Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109867720A (en) * | 2019-04-26 | 2019-06-11 | 兰州兰生血液制品有限公司 | A kind of hyperfiltration process of low Aluminium residual human serum albumin |
| CN114307651A (en) * | 2021-11-23 | 2022-04-12 | 中国辐射防护研究院 | Device and method for separating adsorbed radionuclide colloids with different particle sizes |
-
2007
- 2007-05-30 CN CNU2007200938031U patent/CN201049281Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109867720A (en) * | 2019-04-26 | 2019-06-11 | 兰州兰生血液制品有限公司 | A kind of hyperfiltration process of low Aluminium residual human serum albumin |
| CN114307651A (en) * | 2021-11-23 | 2022-04-12 | 中国辐射防护研究院 | Device and method for separating adsorbed radionuclide colloids with different particle sizes |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080423 |