CN207973744U - Fully-automatic multi-channel cell enrichment instrument - Google Patents
Fully-automatic multi-channel cell enrichment instrument Download PDFInfo
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- CN207973744U CN207973744U CN201721157633.9U CN201721157633U CN207973744U CN 207973744 U CN207973744 U CN 207973744U CN 201721157633 U CN201721157633 U CN 201721157633U CN 207973744 U CN207973744 U CN 207973744U
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Abstract
The utility model is related to a kind of fully-automatic multi-channel cell enrichment instrument, the enriching instrument includes：Rack and n cell enrichment unit for being fixed on the rack, the positive integer that n is >=2；Each cell enrichment unit includes：Cell collecting member, including a filter and the filter membrane, cell collecting member arrival end and the outlet end that are set in the filter；Propulsion positioned at the cell collecting member downstream pumps, for driving liquid to flow；Multi-channel valve, the multi-channel valve is located between the cell collecting member and propulsion pump, and at least provided with first port and second port, first port is connected with cell collecting member outlet end, and second port is connected with pump is promoted；With waste liquid outlet side.The utility model can handle multiple samples, enrichment efficient stable, specificity and reproducible, easy to operate, easy to maintain, the cell activity height of enrichment simultaneously.
Description
Technical field
The utility model is related to cell enrichment instrument, and in particular to a kind of fully-automatic multi-channel cell enrichment instrument.
Background technology
It is enriched with the side of rare maxicell in body fluid (including blood, urine, hydrothorax, ascites etc.) and mixed cell culture at present
Method includes mainly：Centrifugal process, filter membrane method, micro-fluidic chip method, antibody act etc., wherein it is relatively high with filter membrane method bioaccumulation efficiency,
But these methods, as manual operations, not only complex steps, time-consuming, and labor intensity is big, and repeatability and stability are poor,
It can not ensure the requirement to bioaccumulation efficiency and repeated high stability such as clinical detection.Meanwhile traditional filter membrane method mostly uses very
Blank pipe or negative pressure pump rapid filtration under suction can not ensure cell strictly by cell size by membrane retention, and be easy to inhale cause it is thin
Born of the same parents are impaired, and accumulation rate is still relatively low.On the other hand, peristaltic pump such as negative pressure is insufficient, and will produce stream of pulses, pressure in running
It is uneven with flow velocity, still it be easy to cause cell damage deformation, while hose not easy care easy to wear.
In order to solve the defects of prior art, those skilled in the art, which are dedicated to developing one kind, can automate enrichment, simultaneously
Handle that multiple samples, bioaccumulation efficiency be high and stable, reproducible, convenient operating maintenance equipment.
Utility model content
The purpose of the utility model is to overcome the deficiencies in the prior art, and multiple samples, enrichment can be handled simultaneously by being related to one kind
Efficiently, stable, specific and reproducible, easy to operate, easy to maintain fully-automatic multi-channel cell enrichment instrument.
In order to achieve the above object, the utility model provides one kind multichannel cell enrichment instrument, the multichannel enrichment
Instrument includes：Rack and n cell enrichment unit for being fixed on the rack, wherein the positive integer that n is >=2；
Each cell enrichment unit includes：
Cell collecting member, the cell collecting member is used to be enriched with and collect waits for that enrichment is thin in pending sample
Born of the same parents, and the cell collecting member includes：It one filter and is set in the filter and described waits for that enrichment is thin for retaining
The filter membrane of born of the same parents, and the cell collecting member is provided with arrival end and outlet end, wherein the arrival end is waited for for inputting to contain
The pending sample of enrichment of cell or for input be used for washing washing lotion；
Propulsion positioned at the cell collecting member downstream pumps, and the propulsion pump is for driving liquid to flow；
Multi-channel valve, the multi-channel valve are located between the cell collecting member and propulsion pump, and extremely
It is equipped with first port and second port less, wherein the first port is connected with the outlet end of the cell collecting member, and
The second port is connected with propulsion pump；With
Waste liquid outlet side, the waste liquid outlet side is for being discharged waste liquid.
In another preferred example, when the multi-channel valve is in first state, the cell collecting member and institute
The state for promoting pump in fluid connection is stated, is collected so that the propulsion pump drives pending sample to flow through the cell
Component, and flow to propulsion pump；Or the propulsion pump driving washing lotion is made to flow through the cell collecting member, and flow to propulsion
Pump.
In another preferred example, when the multi-channel valve is in the second state, the cell collecting member and institute
The state for promoting pump to be not at fluid connection is stated, so that propulsion pump driving waste liquid flows to waste liquid outlet side.
In another preferred example, propulsion pump is additionally provided with independent waste liquid outlet (the i.e. waste liquid outlet
As the waste liquid outlet side).
In another preferred example, it is additionally provided with switch valve between the propulsion pump and the multi-channel valve, used
In the working condition for switching the propulsion pump.
In another preferred example, the multi-channel valve includes three-way valve or two-way valve.
In another preferred example, the three-way valve is equipped with third port, and the third port is waste liquid outlet side.
In another preferred example, when the three-way valve is in the second state, the second port is pumped with the propulsion
It is connected and third port is connected with waste liquid collection vessel.
In another preferred example, each cell enrichment unit further includes containing the pending sample for waiting for enrichment of cell for containing
The injector of product.
In another preferred example, the cell enrichment instrument further includes the wash liquor vessel for containing washing lotion.
In another preferred example, the injector is syringe.
In another preferred example, the arrival end (i.e. the arrival end of cell collecting member) of each cell enrichment unit and use
It is connected in the outlet end for containing an injector containing the pending sample for waiting for enrichment of cell.
In another preferred example, the arrival end (i.e. the arrival end of cell collecting member) of each cell enrichment unit and use
It is connected in the outlet end for the wash liquor vessel for containing washing lotion.
In another preferred example, it is set between the arrival end and the outlet end of the wash liquor vessel of each cell collecting member
It includes two-port valve and/or check valve to have valve, valve, and the arrival end of cell collecting member is flowed to for controlling washing lotion.
In another preferred example, each cell enrichment unit further includes three-way connection, the first end of the three-way connection
Mouth waits for that the outlet end of the injector of enrichment of cell is connected with for containing to contain, the arrival end of second port and cell collecting member
It is connected, third port is connected with the outlet end of the wash liquor vessel for containing washing lotion.
In another preferred example, valve is equipped between the third port of the three-way connection and the outlet end of the wash liquor vessel
Door, valve includes two-port valve and/or check valve.
In another preferred example, the cell enrichment instrument further includes m wash liquor vessel, and each cell collecting member enters
Mouth end is connected with the outlet end of m wash liquor vessel respectively, wherein the positive integer that the m is >=1.
In another preferred example, the m wash liquor vessel is connected by multiple-pass joint with each cell enrichment unit；
M input terminal of the multiple-pass joint is connected with the outlet end of m wash liquor vessel respectively；N output end of multiple-pass joint point
It is not connected with the third port of the arrival end of each cell collecting member or each three-way connection.
In another preferred example, it is additionally provided with valve between each input terminal and the outlet end of wash liquor vessel of the multiple-pass joint
Door, valve includes two-port valve and/or check valve.
In another preferred example, the valve be arranged multiple-pass joint each input terminal and wash liquor vessel outlet end it
Between, it can avoid pending sample and pollute valve.
In another preferred example, each output end of the multiple-pass joint and the arrival end of each cell collecting member or each three
Valve is additionally provided between pass joint, valve includes two-port valve and/or check valve.
In another preferred example, propulsion pump includes that numerical control promotes pump.
In another preferred example, propulsion pump includes a stepper motor, and the stepper motor promotes pump for driving.
In another preferred example, the stepper motor is lead screw stepper motor.
In another preferred example, propulsion pump further includes a lead screw slide unit, wherein the stepper motor drives lead screw
Slide unit, and driven by lead screw slide unit and promote pump.
In another preferred example, the propulsion pump includes syringe pump.
In another preferred example, it is equipped with liquid containing structure in the propulsion pump, for accommodating the liquid for flowing into and promoting in pump
Body.
In another preferred example, described to promote the volume of the liquid containing structure pumped for 20-100ml.
In another preferred example, the stepper motor is the slowspeed machine of 10ul/min-100ml/min.
In another preferred example, the n cell enrichment unit is mutually in parallel.
In another preferred example, the n cell enrichment unit is separate.
In another preferred example, the filter membrane is by under cell retention to be enriched with, and liquid is allowed to flow through.
In another preferred example, the filter is discoid.
In another preferred example, the aperture of a diameter of 13-25mm of the filter, the filter membrane are 5-20um.
In another preferred example, the cell enrichment instrument further includes fixed frame, and the fixed frame is for fixing the cell
Collecting part.
In another preferred example, the fixed frame is equipped with the filter putting hole for placing filter.
In another preferred example, each valve in the cell enrichment instrument is solenoid valve.
In another preferred example, the multi-channel valve is solenoid valve.
In another preferred example, each valve in the cell enrichment instrument is dismountable.
In another preferred example, the cell enrichment instrument further includes：With the solenoid valve and propulsion pump electrical connection, use
In control solenoid valve and the controller for promoting pump to operate.
In another preferred example, the controller includes host controller and lower level controller, wherein the upper control
The lower level controller of device connection, the lower level controller is transferred to for carrying out artificial parameter setting and control, and by the signal；Institute
It states lower level controller with pump, solenoid valve is promoted to be connected, is sent to host controller for receiving simultaneously conversion sensor signal, and connect
The signal that host controller is sent is received to control the driving solenoid valve and promote pump.
In another preferred example, the lower level controller includes microcontroller, stepper drive, relay.
In another preferred example, the host controller includes microcontroller and display screen.
In another preferred example, the host controller further include have mobile terminal, WIFI, bluetooth module, or combinations thereof,
Cell enrichment instrument described in mobile terminal Wireless remote control can be passed through.
In another preferred example, the cell enrichment instrument further includes sensor (such as gas pressure sensor transmitter module
100KPa, contactless liquid level sensor, temperature sensor, pH sensors), and for passing to the signals such as air pressure, water level
Controller
It should be understood that in the scope of the utility model, above-mentioned each technical characteristic of the utility model and below (such as implementation
Example) in specifically describe each technical characteristic between can be combined with each other, to form a new or preferred technical solution.It is limited to
Length, not repeated them here.
Description of the drawings
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art does and simply introduces, it should be apparent that, the accompanying drawings in the following description is only
It is some embodiments of the utility model, for those of ordinary skill in the art, in the premise not made the creative labor
Under, other drawings may also be obtained based on these drawings.
Fig. 1 shows the structural schematic diagram of the multichannel enriching instrument in one embodiment of the utility model.
Fig. 2 shows the electric operation control circuit figure of the multichannel enriching instrument in one embodiment of the utility model.
Wherein, 1- syringes；2- bottle for handling liquid toilet or cosmetic substance；3- two-port valves；4- cell collecting members；5- three-way magnetic valves；6- promotes pump；
7- waste liquid bottles；8- three-way connections.
Specific implementation mode
The present inventor after extensive and in-depth study, by largely screening, develops a kind of multichannel cell for the first time
Enriching instrument, the multichannel cell enrichment instrument can make to wait for the pending sample low-pressure of enrichment of cell, low flow velocity, continuously slow transit through
The filter membrane of certain area and aperture, makes the cell in sample, by membrane retention, ensure that the spy of enrichment in strict accordance with cell size
Anisotropic, high efficiency and stability, and prevent cell damage from deforming, the cell activity being enriched with is high, especially to rare big
The enrichment of cell.The utility model is additionally provided with the fixed frame of fixed cell collecting member, is more put down when cell being enable to pass through filter membrane
Surely, the cell activity of enrichment further increases.The utility model is also cleaned multiple times by different cleaning solutions different in flow rate, can be removed
Remaining non-maxicell is removed, the cell of target size is obtained.The fully-automatic multi-channel cell enrichment instrument of the utility model can be simultaneously
Multiple samples are handled, enrichment is efficient, stablizes, specific and reproducible, easy to operate, easy to maintain, the cell being enriched with
It is active high, it can be used for enriched tumor cell, stem cell and different functioning cells.
Term
As used herein, term " preferred ", " optimal ", " typical ", " illustratively " or " optionally " does not limit this
The range of utility model or embodiment.
As used herein, term "available" expression include or do not include and/or with or without the use of and/or realize or do not realize
And/or occur or the selection or influence that do not occur, and the selection constitutes some embodiments of the utility model or its consequence
At least partially, without limiting the scope of the utility model.
When enumerating a series of values, merely to it is convenient or succinct, including all possible money range and the range side
Single number in or around boundary.Any numerical value, unless otherwise defined, further include practical approximation, and integer value is not arranged
Except fractional value.Subrange value and practical approximation are not construed as special disclosed value.
Unless the context indicates otherwise, with reference to the object of singulative (such as " something (a thing) " or " this part thing
(the thing) ") it is not excluded for plural form (such as " these things ").
As used herein, term " utility model device " and " the utility model multichannel enriching instrument " are used interchangeably.
As used herein, term " fluid connection ", " can fluid connection " be used interchangeably, and refer to two components or two
It is at a time either directly or indirectly connected by components such as pipelines between port, so that liquid (such as celliferous sample
Product, washing lotion, buffer solution) it can flow between them.It is a kind of typically to realize that the mode of " fluid connection " passes through pipeline (hard tube
And/or hose) be attached.It should be understood that the term further includes that one or more other are arranged in connecting pipe not influence liquid
The component of body connection, these representative components include (but being not limited to)：Valve, pump, probe (such as temperature sensor, pressure
Sensor, pH sensors) etc..
As used herein, term " multi-channel valve " refers to the valve with two or more ports.
As used herein, term " multiple-pass joint " refers to multiple input end, is exported by multiple output ends after collecting
Connector.
The fully-automatic multi-channel cell enrichment instrument of the utility model can the multiple samples of (or concurrently) processing, enrichment simultaneously it is high
Effect is stablized, is specific and reproducible, easy to operate, easy to maintain.
In order to make it easy to understand, providing following operation principle for reference.It should be understood that the scope of protection of the utility model not by
Any restrictions of the operation principle.The main operational principle of the utility model is as follows：
1. after liquid to be detected diluted to a certain concentration, it is collected into 5-50ml syringes, by syringe
It is inserted into one end of three-way connection, the other both ends of three-way connection are separately connected bottle for handling liquid toilet or cosmetic substance outlet end and cell collecting member entrance
End；
2. the filter membrane of different pore size can be placed in filter, the cell for being enriched with different size range.
3. cell collecting member outlet end connects three-way magnetic valve first port, three-way magnetic valve second port connects numerical control
Pump, third port is promoted to lead to waste liquid bottle；Solenoid valve promotes the push-and-pull campaign of pump to realize that liquid crosses film and gives up by cooperation numerical control
Liquid discharges.
4. the one end (washing lotion interface) for the three-way connection being connect with bottle for handling liquid toilet or cosmetic substance outlet end is connected with two-way electromagnetic valve, this valve closes
When conjunction, become the closing cock of three-way connection, cell liquid is preferential in syringe crosses film, and liquid drains in device to be injected, syringe at
For the closing cock of three-way connection, this valve is opened, and washing lotion can enter cell collecting member；
5. the two-way electromagnetic valve other end described in above-mentioned 4 connects check valve, it to be used for counterseal, check valve and multiple-pass joint phase
Even, multiple-pass joint is for connecting a variety of washing lotions and other channels.It is mostly logical according to instrument channel number and the setting of washing lotion type number
The port number of connector.
6. intermediate in bottle for handling liquid toilet or cosmetic substance and the pipeline of multiple-pass joint connection be provided with two-way electromagnetic valve and check valve, respectively with control
Washing lotion leads to the switch of multiple-pass joint and prevents liquid reflux to bottle for handling liquid toilet or cosmetic substance.The switch of this solenoid valve switches for controlling washing lotion,
For normally closed type, when energization, opens, and corresponding washing lotion can be pushed into pumping and enter multiple-pass joint, then by two-way in 4 and check valve into
Enter cell collecting member, is inhaled into propulsion pump.Three-way magnetic valve described in control 3, closes the first port of three-way magnetic valve, beats
Open second port and third port.Finally sucking is promoted the liquid of pump by promoting pump to be discharged into waste liquid bottle.By open successively and
The solenoid valve on bottle for handling liquid toilet or cosmetic substance is closed, realizes that different washing lotions sequentially enters filter cleaning.
7. the opening of all solenoid valves is controlled with the movement closed and propulsion pumps by controller.
The main operational steps of the utility model are as follows：
1. booting：Open power supply
2. the filter membrane in aperture needed for selection, installation to cell collecting member
3. after diluted containing cellular liquid to a certain concentration to be detected, it is collected into 5-50ml syringes, it will
Syringe is inserted into the one end for the three-way connection being connect with cell collecting member arrival end
4. pressing display screen, corresponding channel number, setting or option program is selected to execute according to number of samples
5. after, filter membrane is taken out, completes enrichment.
The major advantage of the utility model
The utility model multichannel cell enrichment instrument can make to wait for the pending sample low-pressure of enrichment of cell, low flow velocity, company
It is continuous to slow transit through certain area and the filter membrane in aperture, so that the cell in sample, by membrane retention, is ensured in strict accordance with cell size
Specificity, high efficiency and the stability of enrichment.And since the slow constant speed of liquid, pressure equably continue through filter membrane, prevent
Only cell damage deforms, and the cell activity being enriched with is high, the especially enrichment to rare maxicell.The utility model is additionally provided with
The fixed frame of fixed cell collecting member, more steady when cell being enable to pass through filter membrane, the cell activity of enrichment further increases.
To which the utility model is also cleaned multiple times by different cleaning solutions different in flow rate, remaining non-maxicell impurity can be removed, is obtained
To the cell of target size.The fully-automatic multi-channel cell enrichment instrument of the utility model can handle multiple samples simultaneously, and enrichment is high
Effect is stablized, is specific and reproducible, easy to operate, easy to maintain.
This fully-automatic multi-channel cell enrichment instrument can be used for separation and concentration body fluid (including blood, urine, hydrothorax, ascites,
Cerebrospinal fluid etc.) in rare maxicell.It is enriched with the different size of cell in mixed cell culture using fenestra size separation.This is complete
Automated multi-channel cell enrichment instrument can also aseptically work, then can to the different size of cell of separation and concentration into
Row culture amplification, analyzes and is applied for subsequent various biological.
With reference to specific embodiment, the utility model is expanded on further.It should be understood that these embodiments are merely to illustrate this
Utility model rather than limitation the scope of the utility model, construction is provided to deform and/or replacing and/or expanding embodiment
Property basis, can differently configuration or change put into practice.In addition, attached drawing is schematic diagram, therefore the utility model fills
It sets and is not limited by the size or ratio of the schematic diagram with equipment.
It should be noted that in the claim and specification of this patent, such as first and second or the like relationship
Term is only used to distinguish one entity or operation from another entity or operation, without necessarily requiring or implying
There are any actual relationship or orders between these entities or operation.Moreover, the terms "include", "comprise" or its
Any other variant is intended to non-exclusive inclusion so that including the processes of a series of elements, method, article or
Equipment includes not only those elements, but also includes other elements that are not explicitly listed, or further include for this process,
Method, article or the intrinsic element of equipment.In the absence of more restrictions, being wanted by what sentence " including one " limited
Element, it is not excluded that there is also other identical elements in the process, method, article or apparatus that includes the element.
Embodiment 1
A kind of multichannel enriching instrument as shown in Figure 1, including：Rack；Be fixed on the rack n are respectively independent mutual
Cell enrichment unit in parallel, wherein the positive integer that n is >=2；Fixed frame for fixing cell collecting member, equipped with being used for
Place the filter putting hole of filter；Waste liquid bottle 7；Bottle for handling liquid toilet or cosmetic substance 2.
Each cell enrichment unit includes：Syringe 1, cell collecting member 4, promotes pump 6, three at three-way connection 8
Three-way electromagnetic valve 5 and waste liquid outlet side.
Syringe 1 contains the pending sample for waiting for enrichment of cell for containing.
Three-way connection 8, first port are connected with the outlet end of syringe 1, and second port enters with cell collecting member 4
Mouth end is connected.
Cell collecting member 4, the enrichment of cell that waits for for being enriched with and collecting in pending sample, including：One filter,
And it is set to the filter membrane in the filter, cell collecting member 4 is additionally provided with arrival end and outlet end.Wherein, filter is
It is discoid, filter a diameter of 13-25mm, filter sizes 5-20um.
Pump 6 is promoted, is located at 4 downstream of cell collecting member, for driving liquid to flow.It further includes stepper motor to promote pump 6
With lead screw slide unit, wherein the stepper motor drives lead screw slide unit, and is driven by lead screw slide unit and promote pump 6.The propulsion pump 6
In be equipped with liquid containing structure, volume 20-100ml, the stepper motor be 10ul/min-100ml/min slowspeed machine.
Three-way magnetic valve 5, between being located at cell collecting member 4 and promoting pump 6, and equipped with first port, second port
And third port.Wherein, first port is connected with the outlet end of the cell collecting member 4, the second port with it is described
Pump 6 is promoted to be connected；Third port is that waste liquid outlet side is connected for waste liquid to be discharged with waste liquid bottle 7.
The m (positive integer that m is >=1) bottle for handling liquid toilet or cosmetic substance 2 are connected by multiple-pass joint with each cell enrichment unit, more
M input terminal of pass joint is connect with m bottle for handling liquid toilet or cosmetic substance 2 respectively, is equipped between each input terminal and bottle for handling liquid toilet or cosmetic substance 2 of multiple-pass joint
Valve, valve can be two-port valve 3 and/or check valve；N output end of multiple-pass joint and the third port phase of each three-way connection 8
Even, the first port of the three-way connection 8 is connected with the outlet end of syringe 1, and second port enters with cell collecting member 4
Mouth end is connected.
When the three-way magnetic valve 5 is in first state, cell collecting member 4 and propulsion pump 6 are in fluid connection
State so that the 6 pending sample of driving of pump is promoted to flow through cell collecting member 4 from syringe 1, and flows to propulsion pump 6；
Or so that 6 driving washing lotion of pump is promoted to flow through cell collecting member 4 from bottle for handling liquid toilet or cosmetic substance 2, and flow to propulsion pump 6.When the threeway
When solenoid valve 5 is in the second state, the cell collecting member 4 and the state for promoting pump 6 to be not at fluid connection, from
And make the third port (waste liquid outlet side) for promoting 6 driving waste liquid of pump to flow to three-way magnetic valve 5, it flows into waste liquid bottle 7.
When the three-way magnetic valve 5 is in first state, the first port and second port of three-way magnetic valve 5 are opened,
Third port is closed, and cell collecting member 4 provides negative pressure with 6 fluid connections of pump, propulsion pump 6 are promoted, and driving liquid flows through cell
Collecting part 4, and flow in the liquid containing structure promoted in pump 6.When the three-way magnetic valve 5 is in the second state, third
Port and second port are opened, and first port is closed, and promote pump 6 and waste liquid outlet side fluid connection.Promote pump 6 driving liquid from
It promotes and is flowed out in the liquid containing structure in pump 6, waste liquid is drained into waste liquid bottle 7 by waste liquid outlet side.
The utility model further includes control system, and control system further includes controller and sensor.
Controller and 6 electrical connection of the solenoid valve and propulsion pump, 6 runnings are pumped for controlling solenoid valve and promoting, including
Host controller and lower level controller, wherein the lower level controller of host controller connection, for carrying out artificial parameter setting
And control, and the signal is transferred to the lower level controller；The lower level controller is connected with pump 6, solenoid valve is promoted, and is used for
It receives and conversion sensor signal is sent to host controller, and receive the signal that host controller is sent to control driving electromagnetism
Valve and propulsion pump 6.The lower level controller includes microcontroller, stepper drive, relay.The host controller includes microcontroller
And display screen, further include having mobile terminal, WIFI and bluetooth module, it can be rich by cell described in mobile terminal Wireless remote control
Collect instrument.
Sensor, such as gas pressure sensor transmitter module 100KPa, contactless liquid level sensor, temperature sensing
Device, pH sensors, and for the signals such as air pressure, water level to be passed to controller.
Embodiment 2
Remaining with embodiment 1, difference lies in：Three-way magnetic valve 5 replaces with two-way electromagnetic valve in each cell enrichment unit.
Two-way electromagnetic valve is equipped with first port, second port.Wherein, the first port and the cell collecting member 4
Outlet end be connected, the second port and the propulsion pump 6 is connected.
Promote pump 6 to be equipped with independent waste liquid outlet (i.e. the waste liquid outlet is as the waste liquid outlet side).
It is additionally provided with switch valve between the propulsion pump 6 and the two-way electromagnetic valve, for switching the work for promoting pump 6
State.
It is incorporated herein by reference in all documents that the utility model refers to, just as each document quilt
It is individually recited as reference.In addition, it should also be understood that, after having read the above-mentioned instruction content of the utility model, this field skill
Art personnel can make various changes or modifications the utility model, and such equivalent forms equally fall within the application appended claims
Book limited range.
Claims (10)
1. a kind of multichannel cell enrichment instrument, which is characterized in that the enriching instrument includes：Rack and it is fixed on the rack
N cell enrichment unit, wherein the positive integer that n is >=2；
Each cell enrichment unit includes：
Cell collecting member, the cell collecting member wait for enrichment of cell for being enriched with and collecting in pending sample,
And the cell collecting member includes：It one filter and is set in the filter and described waits for enrichment of cell for retaining
Filter membrane, and the cell collecting member is provided with arrival end and outlet end, wherein the arrival end waits being enriched with for inputting to contain
The pending sample of cell or for input be used for washing washing lotion；
Propulsion positioned at the cell collecting member downstream pumps, and the propulsion pump is for driving liquid to flow；
Multi-channel valve, the multi-channel valve is located between the cell collecting member and propulsion pump, and at least sets
There are first port and second port, wherein the first port is connected with the outlet end of the cell collecting member, and described
Second port is connected with propulsion pump；With
Waste liquid outlet side, the waste liquid outlet side is for being discharged waste liquid.
2. multichannel cell enrichment instrument as described in claim 1, which is characterized in that the multi-channel valve is equipped with third end
Mouthful, the third port is waste liquid outlet side.
3. multichannel cell enrichment instrument as described in claim 1, which is characterized in that the propulsion pump is additionally provided with independent useless
Liquid outlet, the waste liquid outlet are the waste liquid outlet side.
4. multichannel cell enrichment instrument as described in claim 1, which is characterized in that each cell enrichment unit further includes using
In injector of the splendid attire containing the pending sample for waiting for enrichment of cell；And/or the wash liquor vessel for containing washing lotion.
5. multichannel cell enrichment instrument as described in claim 1, which is characterized in that each cell enrichment unit further includes three
Pass joint, the first port of the three-way connection are connected with the arrival end of cell collecting member, second port with for containing
Containing waiting for that the outlet end of the injector of enrichment of cell is connected, the outlet end phase of third port and the wash liquor vessel for containing washing lotion
Connection.
6. multichannel cell enrichment instrument as described in claim 1, which is characterized in that the cell enrichment instrument further includes m and washes
Liquid container, the positive integer that m is >=1；The m wash liquor vessel is connected by multiple-pass joint with each cell enrichment unit,
M input terminal of the multiple-pass joint is connected with the outlet end of m wash liquor vessel respectively, each input terminal of multiple-pass joint with
Valve is equipped between the outlet end of wash liquor vessel, the valve includes two-port valve and/or check valve；And n of multiple-pass joint
Output end is connected with the arrival end of each cell collecting member respectively.
7. multichannel cell enrichment instrument as described in claim 1, which is characterized in that be equipped with liquid containing knot in the propulsion pump
Structure.
8. multichannel cell enrichment instrument as described in claim 1, which is characterized in that a diameter of 13-25mm of the filter, institute
The aperture for stating filter membrane is 5-20um.
9. multichannel cell enrichment instrument as described in claim 1, which is characterized in that the cell enrichment instrument further includes fixing
Frame, the fixed frame is for fixing the cell collecting member, and the fixed frame is equipped with the filter for placing filter
Putting hole.
10. multichannel cell enrichment instrument as described in claim 1, which is characterized in that the multi-channel valve is solenoid valve, and
And the cell enrichment instrument further includes：With the solenoid valve and promote pump electrical connection, for control solenoid valve and promote pump
The controller of running.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721157633.9U CN207973744U (en) | 2017-09-11 | 2017-09-11 | Fully-automatic multi-channel cell enrichment instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721157633.9U CN207973744U (en) | 2017-09-11 | 2017-09-11 | Fully-automatic multi-channel cell enrichment instrument |
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| Publication Number | Publication Date |
|---|---|
| CN207973744U true CN207973744U (en) | 2018-10-16 |
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| CN201721157633.9U Active CN207973744U (en) | 2017-09-11 | 2017-09-11 | Fully-automatic multi-channel cell enrichment instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111044514A (en) * | 2019-11-27 | 2020-04-21 | 爱威科技股份有限公司 | Enrichment method, sample manufacturing method and sample detection method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111044514A (en) * | 2019-11-27 | 2020-04-21 | 爱威科技股份有限公司 | Enrichment method, sample manufacturing method and sample detection method |
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