CN206799609U - A kind of biofermentation filtration system - Google Patents
A kind of biofermentation filtration system Download PDFInfo
- Publication number
- CN206799609U CN206799609U CN201720594618.4U CN201720594618U CN206799609U CN 206799609 U CN206799609 U CN 206799609U CN 201720594618 U CN201720594618 U CN 201720594618U CN 206799609 U CN206799609 U CN 206799609U
- Authority
- CN
- China
- Prior art keywords
- pipeline
- triple valve
- ball valve
- biofermentation
- hollow fiber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/12—Apparatus for enzymology or microbiology with sterilisation, filtration or dialysis means
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The utility model is a kind of biofermentation filtration system, including tank body, peristaltic pump, two hollow fiber film assemblies, first triple valve, second triple valve, 3rd triple valve, first pipeline, second pipe, 3rd pipeline, 4th pipeline, 5th pipeline and the 6th pipeline, the feedback outlet of two hollow fiber film assemblies is connected by the first pipeline, it is intersected in the 5th pipeline, the charging aperture of two hollow fiber film assemblies is connected by second pipe, it is intersected in the 4th pipeline, the filtered solution outlet of two hollow fiber film assemblies is connected by the 3rd pipeline, it is intersected in the 6th pipeline, tank base is equiped with the first triple valve, the first interface of first triple valve is connected with tank body, the second interface of first triple valve is connected by the 4th pipeline through peristaltic pump with second pipe, 3rd interface of the first triple valve is as discharge gate.The utility model convenient disassembly, is readily cleaned.
Description
Technical field
It the utility model is related to a kind of filtration system, more particularly to a kind of biofermentation filtration system.
Background technology
With the development of technique for gene engineering, by transforming microbial gene, engineering bacteria is allowed（Such as Escherichia coli, saccharomycete
Deng）The direct product such as secretory protein or polypeptide is into fermented liquid supernatant liquid, it has also become a kind of conventional meanses.Zymotic fluid is carried out
Bacterium solution separates, and obtains fermented liquid supernatant liquid, and traditional separation method is by tubular type continuous flow centrifuge, is enriched with thalline, separation
Go out fermented supernatant fluid.But there is also some problems for it：After bacteria liquid separation, it is difficult to which whether control supernatant also remains thalline；
Bowl volume is small, frequent shutdown, removes bacteria residue, intermittently operated, influences efficiency；Operation power consumption is big, and noise is big.
At present at home for be adapted to laboratory scale or pilot-scale do micro-filtration it is degerming or be concentrated by ultrafiltration it is designed in
Empty fiber membrane filtration system, the problem of it is present be：The hollow fiber film assembly of fixed frame assembling, dismounting are inconvenient, it is impossible to
According to different preparation technologies, neatly go to change the hollow fiber film assembly of different size；Storage tank is also individually to separate, even
After connecing the pump housing, mobile inconvenience is caused.
The content of the invention
The purpose of this utility model is to consider above mentioned problem and provide a kind of biofermentation filtration system.The utility model
It is simple in construction, convenient disassembly, it is readily cleaned, is a kind of moveable biofermentation filtration system, changes the hollow of different size
Fiber film component, micro-filtration aseptic filtration can be carried out to microbial fermentation solution, can also carry out ultrafiltration to the filtered solution product after degerming
Concentration.
The technical solution of the utility model is：Biofermentation filtration system of the present utility model, include tank body, wriggle
Pump, two hollow fiber film assemblies, the first triple valve, the second triple valve, the 3rd triple valve, the first pipeline, second pipe, the 3rd
Pipeline, the 4th pipeline, the 5th pipeline and the 6th pipeline, the feedback outlet of two of which hollow fiber film assembly are connected by the first pipeline
Connecing, be intersected in the 5th pipeline, the charging aperture of two hollow fiber film assemblies is connected by second pipe, is intersected in the 4th pipeline,
The filtered solution outlet of two hollow fiber film assemblies is connected by the 3rd pipeline, is intersected in the 6th pipeline, the bottom installing of tank body
There is the first triple valve, the first interface of the first triple valve is connected with tank body, and the second interface of the first triple valve passes through the 4th pipeline
It is connected through peristaltic pump with second pipe, the 3rd interface of the first triple valve is as discharge gate.
The utility model is simple in construction, convenient disassembly, is readily cleaned, moveable biofermentation filtration system, changes not
The hollow fiber film assembly of same specification, micro-filtration aseptic filtration can be carried out to microbial fermentation solution, also can be to the filtered solution after degerming
Product is concentrated by ultrafiltration.In addition, the microfiltration membranes of hollow fiber film assembly can be used for micro-filtration to remove engineering bacteria（As Escherichia coli,
Saccharomycete etc.）.The milipore filter of the hollow fiber film assembly can be used for the biological heteropolymer in concentration different molecular weight section（Such as
Polyglutamic acid, plant polyose etc.）.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model；
Fig. 2 is coupling part structural representation corresponding to first opening on tank body top；
Fig. 3 is coupling part structural representation corresponding to second opening on tank body top；
Fig. 4 is coupling part structural representation corresponding to tank base opening.
Embodiment
The utility model is described in further detail with specific embodiment below in conjunction with the accompanying drawings, but protection model of the present utility model
Enclose not limited to this.
Biofermentation filtration system of the present utility model, include tank body 1,2, two hollow fiber film assemblies 3 of peristaltic pump,
First triple valve 51, the second triple valve 52, the 3rd triple valve 53, the first pipeline 61, second pipe 62, the 3rd pipeline the 63, the 4th
Pipeline 71, the 5th pipeline 72 and the 6th pipeline 73, the feedback outlet 32 of two of which hollow fiber film assembly 3 pass through the first pipeline 61
Connection, is intersected in the 5th pipeline 72, the charging aperture 31 of two hollow fiber film assemblies 3 is connected by second pipe 62, is intersected in
4th pipeline 71, the filtered solution outlet 33 of two hollow fiber film assemblies 3 are connected by the 3rd pipeline 63, are intersected in the 6th pipeline
73, the bottom of tank body 1 is equiped with the first triple valve 51, and the first interface of the first triple valve 51 is connected with tank body 1, the first triple valve
51 second interface is connected by the 4th pipeline 71 through peristaltic pump 2 with second pipe 62, and the 3rd interface of the first triple valve 51 is made
For discharge gate.
In addition, the top of above-mentioned tank body 1 is provided with cover 15.In the present embodiment, the one of above-mentioned cover 15 is semifixed, half energy
Can flexibly it uncap.
In the present embodiment, above-mentioned tank body 1 is stainless steel tank body, and its side wall upper part is provided with the first opening, is filled in the first opening
Second Hygiene Ball Valve 9, the second triple valve 52 and the 3rd Hygiene Ball Valve 10.Second Hygiene Ball Valve 9 connects the first opening, the second camphor ball
Another the second triple valve of side joint 52 of valve 9, the horizontal opening of the second triple valve 52 connect the 5th pipeline 72, the vertical openings of the second triple valve 52
The 3rd Hygiene Ball Valve 10 is connect, as discharge gate.See Fig. 2.
In the present embodiment, the above-mentioned side wall upper part of tank body 1 is provided with the second opening, and the 4th Hygiene Ball Valve is equiped with the second opening
11st, the 3rd triple valve 53 and the 5th Hygiene Ball Valve 12.4th Hygiene Ball Valve 11 connects the second opening, the opposite side of the 4th Hygiene Ball Valve 11
The 3rd triple valve 53 is connect, the horizontal opening of the 3rd triple valve 53 connects the 6th pipeline 73, and the vertical openings of the 3rd triple valve 53 connect the 5th and defended
Green-ball valve 12, as discharge gate.See Fig. 3.
In the present embodiment, above-mentioned 4th pipeline 71 is connected through peristaltic pump 2 with second pipe 62, the other end of the 4th pipeline 71 with
First Hygiene Ball Valve 8 connects.
In the present embodiment, above-mentioned first pipeline 61, the pipeline 63 of second pipe 62 and the 3rd are pvc pipes.
In the present embodiment, above-mentioned 4th pipeline 71, the 5th pipeline 72 and the 6th pipeline 73 are silicone tubes.
In the implementation case, the bottom of above-mentioned tank body 1 is equiped with the first triple valve 51, the first interface of the first triple valve 51
It is connected with tank body 1, the second interface of the first triple valve 51 is connected by the 4th pipeline 71 through peristaltic pump 2 with second pipe 62, the
3rd interface of one triple valve 51 is as larger discharge gate.See Fig. 4.
In the present embodiment, the bottom of above-mentioned tank body 1 is equiped with three support feets, and three support feets are equiped with three ten thousand respectively
To wheel 14, the tank skin of tank body 1 is equiped with handle.
In the present embodiment, above-mentioned hollow fiber film assembly 3 is equiped with micro-filtration hollow fiber film assembly or ultrafiltration hollow fiber
Membrane module.
In the present embodiment, the pore size filter of microfiltration membranes is set by above-mentioned hollow fiber film assembly 3:0.001 μm~0.12 μ
m.From two 0.2 μm of apertures.
The hollow fiber film assembly 3, charging aperture 31, feedback outlet 32, filtered solution are all exported 33 respectively by pvc pipe,
Parallel connection, which communicates, to cross to charging aperture house steward, feedback outlet house steward, filtered solution outlet header.Charging aperture house steward and feedback outlet house steward installation
There is pressure gauge, pressure gauge scope is in 0.1~0.6MPa.
Operation principle of the present utility model is：Filtration system is moved to by fermentation tank, the upper lid of tank body 1 is opened, by
The escherichia coli fermented broth of fermented end is sent in tank body 1 through pipeline by pressure inside the tank, open the first Hygiene Ball Valve 8,
5th Hygiene Ball Valve 12, the 3rd Hygiene Ball Valve 10, the 4th Hygiene Ball Valve 11, the 6th Hygiene Ball Valve 13 are closed, in filter process, defended
The second Hygiene Ball Valve of green-ball valve 9 is in adjustable state, is no more than 0.2MPa for control pressure list index reading.By wriggling
The conveying of pump 2, Hygiene Ball Valve fiveth Hygiene Ball Valve 12 outlet outflow of the micro-filtrate through open mode, is obtained containing protein or polypeptide
The fermented supernatant fluid of product.
After filtering terminates, the first Hygiene Ball Valve 8 is opened, the 3rd Hygiene Ball Valve 10, the 5th Hygiene Ball Valve 12, the 4th is closed and defends
Green-ball valve 11, the 6th Hygiene Ball Valve 13, the second Hygiene Ball Valve 9 are in adjustable state, clear water are poured into tank body, washed away hollow
Remaining waste in fiber film component 3.
After washing away the remaining waste in hollow fiber film assembly 3, the first Hygiene Ball Valve 8 is opened, the 4th Hygiene Ball Valve 11, is closed
Closing the 3rd Hygiene Ball Valve 10, the 5th Hygiene Ball Valve 12, the 6th Hygiene Ball Valve 13, the second Hygiene Ball Valve 9 is in adjustable state, then
Backflow is carried out with acid or alkaline rinse to rinse about 1 hour.
After carrying out backflow flushing about 1 hour with acid or alkaline rinse, the first Hygiene Ball Valve 8, the 3rd Hygiene Ball Valve are opened
10, the 5th Hygiene Ball Valve 12 closes the 4th Hygiene Ball Valve 11, the 6th Hygiene Ball Valve 13, and the second Hygiene Ball Valve 9 is in adjustable nodular
State, clear water is poured into tank body, wash away the acidity or alkaline rinse of the residual in hollow fiber film assembly 3.
The acidity or alkaline rinse of the residual in hollow fiber film assembly 3 are washed away with clear water, with pH reagent test water quality pH
Value about 7 or so, opens the first Hygiene Ball Valve 8, the 4th Hygiene Ball Valve 11, closes the 3rd Hygiene Ball Valve 10, the 5th Hygiene Ball Valve
12, the 6th Hygiene Ball Valve 13, the second Hygiene Ball Valve 9 is in adjustable state, then is flowed back with the sodium hypochlorite of suitable concentration
Rinse, allow hollow fiber film assembly 3 is interior to preserve liquid full of sodium hypochlorite, that is, complete cleaning and preservation work.
Claims (10)
1. a kind of biofermentation filtration system, it is characterised in that include tank body, peristaltic pump, two hollow fiber film assemblies,
One triple valve, the second triple valve, the 3rd triple valve, the first pipeline, second pipe, the 3rd pipeline, the 4th pipeline, the 5th pipeline and
6th pipeline, the feedback outlet of two of which hollow fiber film assembly are connected by the first pipeline, are intersected in the 5th pipeline, in two
The charging aperture of empty fiber membrane component is connected by second pipe, is intersected in the 4th pipeline, the filtration of two hollow fiber film assemblies
Liquid outlet is connected by the 3rd pipeline, is intersected in the 6th pipeline, the bottom of tank body is equiped with the first triple valve, the first triple valve
First interface is connected with tank body, and the second interface of the first triple valve is connected by the 4th pipeline through peristaltic pump with second pipe, the
3rd interface of one triple valve is as discharge gate.
2. biofermentation filtration system according to claim 1, it is characterised in that the top of above-mentioned tank body is provided with cover.
3. biofermentation filtration system according to claim 1, it is characterised in that above-mentioned tank wall top is provided with first
It is open, the second Hygiene Ball Valve, the second triple valve and the 3rd Hygiene Ball Valve, the second Hygiene Ball Valve cans picking body is equiped with the first opening
First opening of side wall, the triple valve of another side joint second of the second Hygiene Ball Valve, the horizontal opening of the second triple valve connect the 5th pipe
Road, the vertical openings of the second triple valve connect the 3rd Hygiene Ball Valve, as discharge gate.
4. biofermentation filtration system according to claim 1, it is characterised in that above-mentioned tank wall top is provided with second
It is open, the 4th Hygiene Ball Valve, the 3rd triple valve and the 5th Hygiene Ball Valve, the 4th Hygiene Ball Valve cans picking body is equiped with the second opening
The second opening in side wall, the triple valve of another side joint the 3rd of the 4th Hygiene Ball Valve, the horizontal opening of the 3rd triple valve connect the 6th
Pipeline, the vertical openings of the 3rd triple valve connect the 5th Hygiene Ball Valve, as discharge gate.
5. biofermentation filtration system according to claim 1, it is characterised in that the other end of above-mentioned 4th pipeline and
One Hygiene Ball Valve connects.
6. the biofermentation filtration system according to any one of claim 1 to 5, it is characterised in that above-mentioned first pipeline,
Two pipelines and the 3rd pipeline are pvc pipes；Above-mentioned 4th pipeline, the 5th pipeline and the 6th pipeline are silicone tubes.
7. biofermentation filtration system according to claim 6, it is characterised in that the bottom of above-mentioned tank body is equiped with three
Support feet, three support feets are equiped with three universal wheels respectively, and the tank skin of tank body is equiped with handle.
8. biofermentation filtration system according to claim 6, it is characterised in that above-mentioned hollow fiber film assembly is micro-filtration
Hollow fiber film assembly or ultra filtration hollow fiber membrane component.
9. biofermentation filtration system according to claim 8, it is characterised in that micro- set by above-mentioned hollow fiber film assembly
The pore size filter of filter membrane is:0.001 μm~0.12 μm.
10. biofermentation filtration system according to claim 9, it is characterised in that surpass set by above-mentioned hollow fiber film assembly
Membrane filtration aperture is:0.15 μm~0.85 μm.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720594618.4U CN206799609U (en) | 2017-05-25 | 2017-05-25 | A kind of biofermentation filtration system |
| PCT/CN2017/099149 WO2018214317A1 (en) | 2017-05-25 | 2017-08-25 | Bio-fermentation filtration system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720594618.4U CN206799609U (en) | 2017-05-25 | 2017-05-25 | A kind of biofermentation filtration system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206799609U true CN206799609U (en) | 2017-12-26 |
Family
ID=60742637
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720594618.4U Expired - Fee Related CN206799609U (en) | 2017-05-25 | 2017-05-25 | A kind of biofermentation filtration system |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN206799609U (en) |
| WO (1) | WO2018214317A1 (en) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201850276U (en) * | 2010-05-07 | 2011-06-01 | 江苏大学 | Micro-filtration membrane coupling high-density culture device for lactic acid bacteria |
| CN201873680U (en) * | 2010-07-07 | 2011-06-22 | 中国石油化工股份有限公司 | System device for ethanol fermentation and separation and coupling based on pervaporation membrane |
| CN202509078U (en) * | 2012-03-08 | 2012-10-31 | 上海理工大学 | Lactic acid fermentation device based on dual-bacterium fermentation |
-
2017
- 2017-05-25 CN CN201720594618.4U patent/CN206799609U/en not_active Expired - Fee Related
- 2017-08-25 WO PCT/CN2017/099149 patent/WO2018214317A1/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| WO2018214317A1 (en) | 2018-11-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102153251B (en) | External anaerobic membrane bioreactor and waste water purification technology thereof | |
| CN102616954B (en) | Integrated water purification and supply method and system | |
| CN207362235U (en) | Anaerobic fermentation system | |
| CN207928807U (en) | A kind of automatic backwash bag type filter device | |
| CN206799609U (en) | A kind of biofermentation filtration system | |
| CN103173347A (en) | Multifunctional continuous fermentation and extraction integrated apparatus | |
| CN211612279U (en) | Membrane separation device with cleaning function | |
| CN204952681U (en) | Improved generation rubbish is washed and is swept automobile -used membrane separation device | |
| CN206511963U (en) | A kind of water purifier and water cleaning systems | |
| CN202983520U (en) | Fully-enclosed aseptic liquid medicine ultra-filtration tank device | |
| CN102527242B (en) | Sanitary external pressure hollow fiber membrane assembly water collecting device | |
| CN207918494U (en) | A kind of economical pressure type drinking water deep purification membrane-filtering unit | |
| CN208008570U (en) | Water purifier under a kind of kitchen | |
| CN207031442U (en) | Aerobic fermentation system | |
| CN216498593U (en) | Self-cleaning multifunctional tubular separation membrane equipment | |
| CN104017725A (en) | Stirring gravity type fermentation and extraction integrated tank and extraction method realized by using same | |
| CN204602003U (en) | A kind of feed liquid ultrafiltration concentration equipment | |
| CN206082189U (en) | Portable biofiltration device that declines | |
| CN203212558U (en) | Multifunctional continuous fermenting and extracting integrated device | |
| CN205516790U (en) | Canning of sewage purification plastic steel is put | |
| CN205252910U (en) | Equipment that technology drainage of running water water factory was retrieved | |
| CN212068367U (en) | Ceramic membrane cleaning device | |
| CN208893782U (en) | A kind of waste liquid filtering recovering device of board-washing machine | |
| CN104031833A (en) | Clutch gravity type fermentation and extraction integrated tank and extraction method thereof | |
| CN103041706A (en) | Totally-enclosed ultrafiltration tank device for sterile liquid medicine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171226 Termination date: 20200525 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |