CN208200794U - A kind of high-precision protein purification device - Google Patents
A kind of high-precision protein purification device Download PDFInfo
- Publication number
- CN208200794U CN208200794U CN201820181398.7U CN201820181398U CN208200794U CN 208200794 U CN208200794 U CN 208200794U CN 201820181398 U CN201820181398 U CN 201820181398U CN 208200794 U CN208200794 U CN 208200794U
- Authority
- CN
- China
- Prior art keywords
- nickel column
- bottle
- column pipeline
- protein purification
- purification device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Peptides Or Proteins (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The utility model discloses a kind of high-precision protein purification devices, including nickel column pipeline and the feed liquor station being connect with the inlet of nickel column pipeline, the liquid outlet of nickel column pipeline is provided with triple valve, triple valve is also connected with waste liquid bottle and pure protein liquid bottle, filter membrane is respectively equipped with above and below nickel column particle in nickel column pipeline, the lower section liquid outlet position of nickel column pipeline is funnel-form, and the inlet of the nickel column pipeline is equipped with dispenser, and the dispenser is to be uniformly distributed foraminate round knee-piece；The high-precision protein purification device structure of the utility model is simple, easy to use, low in cost and suitable popularization, and the upper surface of liquid covering nickel column particle can be made to can also ensure that each position can be all flushed to when rinsing, keep purification effect more preferable.
Description
Technical field
The utility model relates to bio-instruments fields, more particularly to a kind of high-precision protein purification device.
Background technique
Isolating and purifying for protein uses extensively in biochemical research application, is an important operating technology.For
Study some protein, it is necessary to first be purified the protein from other protein and non-proteinaceous molecule.So
And the device for carrying out protein purification at present is all that structure is more complicated, price costly, is unfavorable for promoting and use.
And in the prior art, protein purification device use is not convenient enough, and effect has to be hoisted.
Therefore it is badly in need of a kind of new protein purification device, to solve problems of the prior art.
Utility model content
The purpose of the utility model is to overcome deficiency in the prior art, provide a kind of structure it is simple, it is easy to use, at
The high-precision protein purification device of this cheap and suitable popularization.
To achieve the above object, technical solution used by the utility model is as follows: a kind of high-precision protein purification device,
Nickel column pipeline and the feed liquor station connecting with the inlet of nickel column pipeline, the liquid outlet of nickel column pipeline are provided with triple valve,
Triple valve is also connected with waste liquid bottle and pure protein liquid bottle, is respectively equipped with filtering above and below the nickel column particle in nickel column pipeline
The lower section liquid outlet position of film, nickel column pipeline is funnel-form, and the inlet of the nickel column pipeline is equipped with dispenser, the liquid separation
Device is to be uniformly distributed foraminate round knee-piece.
Preferably, stoste bottle, waste liquid cleaning liquid bottle and albumen cleaning liquid bottle are provided on feed liquor station, stoste bottle gives up
The liquid outlet of liquid cleaning liquid bottle and albumen cleaning liquid bottle is connect with the inlet of nickel column pipeline and alternating feed liquor.
Preferably, the liquid outlet of stoste bottle, waste liquid cleaning liquid bottle and albumen cleaning liquid bottle is respectively connected with switch valve, switch
Valve is connect with the inlet of nickel column pipeline.
Preferably, switch valve is 3-position-3-way solenoid valve.
Preferably, high-precision protein purification device further includes the controller for control switch valve and threeway threshold switch.
Preferably, display screen is provided on controller.
Compared with prior art, the utility model is provided with triple valve in the liquid outlet of nickel column pipeline, and triple valve is also connected with
There are waste liquid bottle and pure protein liquid bottle, it may be convenient to the nickel column by waste liquid and pure protein liquid separate collection, in nickel column pipeline
Filter membrane is respectively equipped with above and below grain, the mesh diameter of filter membrane is less than the diameter of nickel column particle, can keep nickel column bed
It is steady, by the lower section liquid outlet position of nickel column pipeline be funnel-form, liquid is without dead angle out.The utility model structure is simple, uses
Convenient, low in cost and suitable popularization.
The utility model can make the upper surface of liquid covering nickel column particle can also ensure that each position when rinsing
It can all be flushed to, keep purification effect more preferable.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model；
Fig. 2 is the main view of the utility model dispenser.
Specific embodiment
The technical issues of in order to keep the utility model solved, technical solution and beneficial effect are more clearly understood, below
In conjunction with accompanying drawings and embodiments, the utility model is described in further detail.It should be appreciated that specific reality described herein
It applies example to be only used to explain the utility model, is not used to limit the utility model.
As shown in the structural schematic diagram of Fig. 1 the utility model, a kind of high-precision protein purification device, including nickel column pipeline 1
And the feed liquor station 2 connecting with the inlet of nickel column pipeline 1, the liquid outlet of nickel column pipeline 1 are provided with triple valve 4, triple valve 4 is also
It is connected with waste liquid bottle 3 and pure protein liquid bottle 5, is respectively equipped with filter membrane 11, nickel above and below the nickel column particle in nickel column pipeline 1
The lower section liquid outlet position on column tube road 1 is funnel-form.Albumen stoste is after the inflow nickel column pipeline 1 of feed liquor station 2, in filter membrane 11
Buffering under slowly invade in nickel column particle, the mesh diameter of filter membrane 11 is less than the diameter of nickel column particle, the mistake below nickel column
Filter membrane 11 is for preventing nickel column particle from flowing out from liquid outlet, and after nickel column pipeline, waste liquid enters useless liquid from liquid outlet outflow
It is funnel-form by the lower section liquid outlet position of nickel column pipeline 1, liquid is without dead angle out in liquid bottle 3.The inlet of the nickel column pipeline 1
Dispenser 101 is installed, the dispenser 101 is the circle knee-piece for being evenly distributed with aperture 102.
As shown in the main view of Fig. 2 the utility model dispenser, multiple apertures are evenly distributed on the dispenser 101
102, and side view is an arc surface, which makes the liquid direction out of the aperture 102 that can make liquid towards multiple angles
The upper surface for covering nickel column particle can also ensure that each position can be all flushed to, keep purification effect more preferable when rinsing.
The aperture 102
In the present embodiment, stoste bottle 21, waste liquid cleaning liquid bottle 22 and albumen cleaning liquid bottle are provided on feed liquor station 2
23, the liquid outlet of stoste bottle 21, waste liquid cleaning liquid bottle 22 and albumen cleaning liquid bottle 23 is connect with the inlet of nickel column pipeline 1
And alternating feed liquor.Stoste bottle 21 goes out liquid, and a large amount of waste liquid is flowed out from liquid outlet, into waste liquid bottle 3, the albumen that needs and a small amount of
Impurity is attached in nickel column, and that closes stoste bottle 21 goes out liquid, opens waste liquid cleaning liquid bottle 22, and the impurity being attached in nickel column on a small quantity is rushed
It washes off, flows into waste liquid bottle 3, until closing waste liquid cleaning liquid bottle 22 after flushing loses no time, open albumen cleaning liquid bottle 23, at this point,
Triple valve 4 is opened to other direction, albumen flushing liquor rinses away the albumen of needs, into pure protein liquid bottle 5.
In the present embodiment, the liquid outlet of stoste bottle 21, waste liquid cleaning liquid bottle 22 and albumen cleaning liquid bottle 23 is respectively connected with
Switch valve 24, switch valve 24 are connect with the inlet of nickel column pipeline 1.
In the present embodiment, switch valve 24 is 3-position-3-way solenoid valve.
In the present embodiment, high-precision protein purification device further includes the control switched for control switch valve 24 and triple valve 4
Device 6 processed.
In the present embodiment, display screen is provided on controller 6.
The above is only the preferred embodiment of the utility model only, is not intended to limit the utility model, all at this
Made any modifications, equivalent replacements, and improvements etc., should be included in the utility model within the spirit and principle of utility model
Protection scope within.
Claims (6)
1. a kind of high-precision protein purification device, including nickel column pipeline (1) and the feed liquor being connect with the inlet of nickel column pipeline (1)
Station (2), it is characterised in that: the liquid outlet of the nickel column pipeline (1) is provided with triple valve (4), and the triple valve (4) is also connected with
There are waste liquid bottle (3) and pure protein liquid bottle (5), is respectively equipped with filtering above and below the nickel column particle in the nickel column pipeline (1)
The lower section liquid outlet position of film (11), the nickel column pipeline (1) is funnel-form, and the inlet of the nickel column pipeline (1) is equipped with
Dispenser (101), the dispenser (101) are the circle knee-piece for being evenly distributed with aperture (102).
2. high-precision protein purification device according to claim 1, it is characterised in that: be arranged on the feed liquor station (2)
There are stoste bottle (21), waste liquid cleaning liquid bottle (22) and albumen cleaning liquid bottle (23), the stoste bottle (21), waste liquid cleaning liquid bottle
(22) and the liquid outlet of albumen cleaning liquid bottle (23) is connect with the inlet of the nickel column pipeline (1) and alternating feed liquor.
3. high-precision protein purification device according to claim 2, it is characterised in that: the stoste bottle (21), waste liquid punching
The liquid outlet of bottle for handling liquid toilet or cosmetic substance (22) and albumen cleaning liquid bottle (23) is respectively connected with switch valve (24), the switch valve (24) with it is described
The inlet of nickel column pipeline (1) connects.
4. high-precision protein purification device according to claim 3, it is characterised in that: the switch valve (24) is three three
Three-way electromagnetic valve.
5. high-precision protein purification device according to claim 4, it is characterised in that: the high-precision protein purification device
It further include the controller (6) for controlling the switch valve (24) and the triple valve (4) switch.
6. high-precision protein purification device according to claim 5, it is characterised in that: be provided on the controller (6)
Display screen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820181398.7U CN208200794U (en) | 2018-10-29 | 2018-10-29 | A kind of high-precision protein purification device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820181398.7U CN208200794U (en) | 2018-10-29 | 2018-10-29 | A kind of high-precision protein purification device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208200794U true CN208200794U (en) | 2018-12-07 |
Family
ID=64534653
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820181398.7U Active CN208200794U (en) | 2018-10-29 | 2018-10-29 | A kind of high-precision protein purification device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208200794U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115105860A (en) * | 2022-07-21 | 2022-09-27 | 成都德傲生物科技有限公司 | Protein purification nickel column regeneration method and system |
-
2018
- 2018-10-29 CN CN201820181398.7U patent/CN208200794U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115105860A (en) * | 2022-07-21 | 2022-09-27 | 成都德傲生物科技有限公司 | Protein purification nickel column regeneration method and system |
| CN115105860B (en) * | 2022-07-21 | 2024-01-09 | 成都德傲生物科技有限公司 | Protein purification nickel column regeneration method and system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101961578B (en) | Novel water treatment system | |
| CN102644769B (en) | Multifunctional control valve | |
| CN208200794U (en) | A kind of high-precision protein purification device | |
| CN105129915B (en) | A kind of water purification machine and its method for improving water quality when turning back on | |
| CN109364525A (en) | A kind of mesenchyma excretion body separating extraction device and its method | |
| CN108946885A (en) | A kind of water treatment system and water purifier | |
| CN209242970U (en) | A kind of peptide purification preparation facilities | |
| CN207918731U (en) | A kind of novel protein purification devices | |
| EP3642624A1 (en) | Combinatory separation | |
| CN208641896U (en) | A kind of integral type is from rinsing purifier | |
| CN208660837U (en) | One Plant Extracts Multistage filtering device | |
| CN208626788U (en) | A kind of filter assemblies, urine filtering device and urine filtering recovery system | |
| CN102644768B (en) | Multifunctional control valve | |
| CN209537141U (en) | Water purification system and water purifier with it | |
| CN209508207U (en) | A kind of magnetic beads for purifying system of fed-batch test | |
| CN208893986U (en) | Purifier with ultrafiltration membrane stack | |
| CN207816912U (en) | A kind of laboratory separation and purification of protein system | |
| WO2020083373A1 (en) | Large-scale magnetic purification system | |
| CN205483751U (en) | Synthetic colorant of food draws purification device | |
| CN209816004U (en) | Monoclonal antibody purification column and purification device using same | |
| CN110218640A (en) | A kind of aerosol Acquisition Instrument automatic sample micropipette device | |
| CN209797824U (en) | Automatic purification system | |
| CN208440304U (en) | A kind of flusher of interior influent stream fine fack spotter | |
| CN212127879U (en) | Aerobic sewage purification device for environmental protection | |
| CN206308296U (en) | A kind of device for connecting protein purification chromatographic column and desalination chromatographic column |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |