CN110218640A - A kind of aerosol Acquisition Instrument automatic sample micropipette device - Google Patents
A kind of aerosol Acquisition Instrument automatic sample micropipette device Download PDFInfo
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- CN110218640A CN110218640A CN201910244305.XA CN201910244305A CN110218640A CN 110218640 A CN110218640 A CN 110218640A CN 201910244305 A CN201910244305 A CN 201910244305A CN 110218640 A CN110218640 A CN 110218640A
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- sample bottle
- buffer
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- 239000000443 aerosol Substances 0.000 title claims abstract description 32
- 239000007788 liquid Substances 0.000 claims abstract description 101
- 238000004140 cleaning Methods 0.000 claims abstract description 78
- 239000012530 fluid Substances 0.000 claims abstract description 34
- 239000002699 waste material Substances 0.000 claims description 105
- 239000000872 buffer Substances 0.000 claims description 57
- 238000002347 injection Methods 0.000 claims description 56
- 239000007924 injection Substances 0.000 claims description 56
- 239000007853 buffer solution Substances 0.000 claims description 43
- 239000000243 solution Substances 0.000 claims description 35
- 238000007599 discharging Methods 0.000 claims description 27
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 20
- 230000002572 peristaltic effect Effects 0.000 claims description 6
- 230000003139 buffering effect Effects 0.000 claims 3
- 239000006210 lotion Substances 0.000 claims 2
- 238000001514 detection method Methods 0.000 abstract description 10
- 238000000034 method Methods 0.000 abstract description 9
- 208000015181 infectious disease Diseases 0.000 abstract description 8
- 238000012544 monitoring process Methods 0.000 abstract description 8
- 230000000813 microbial effect Effects 0.000 abstract description 7
- 238000001802 infusion Methods 0.000 abstract description 6
- 244000005700 microbiome Species 0.000 abstract description 6
- 238000005070 sampling Methods 0.000 abstract description 4
- 208000035473 Communicable disease Diseases 0.000 abstract description 3
- 238000003975 animal breeding Methods 0.000 abstract description 3
- -1 cleaning Substances 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 239000007791 liquid phase Substances 0.000 abstract description 3
- 238000013461 design Methods 0.000 abstract description 2
- 206010011409 Cross infection Diseases 0.000 abstract 1
- 239000002245 particle Substances 0.000 description 5
- 230000001717 pathogenic effect Effects 0.000 description 5
- 241000700605 Viruses Species 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 244000052769 pathogen Species 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 206010069767 H1N1 influenza Diseases 0.000 description 2
- 206010064097 avian influenza Diseases 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000000241 respiratory effect Effects 0.000 description 2
- 201000010740 swine influenza Diseases 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 241000712461 unidentified influenza virus Species 0.000 description 2
- 241000711573 Coronaviridae Species 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000025370 Middle East respiratory syndrome Diseases 0.000 description 1
- 231100000678 Mycotoxin Toxicity 0.000 description 1
- 238000003915 air pollution Methods 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
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- 238000002474 experimental method Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
Abstract
The invention discloses a kind of aerosol Acquisition Instrument automatic sample micropipette devices, increase and change liquid, cleaning and fluid replacing unit, can carry out changing liquid, cleaning and fluid infusion to sample bottle automatically.The present invention is for the connectivity problem after the sampling of bioaerosol Acquisition Instrument with biological detection equipment, pass through innovative design, it establishes the fast trace liquid relief for liquid phase microorganism sampler downstream sample, change liquid, cleaning, fluid infusion system, a full set of process can be completed in 45 seconds.This set system can make microbial aerosol capture and detection apparatus is unattended can be more suitable in large-scale assembly, emphasis stream of people's logistics place etc..Meet the monitoring capability of strategic area antibioterrorism microbial aerosol, and the stream of peoples such as inside-hospital infection, animal-breeding field, entry and exit of the port border hall, the vehicles are densely carried out with the technology needs of full-automatic infectious disease environmental microorganism aerosol monitoring.
Description
Technical field
The present invention relates to field of biotechnology, specially it is a kind of can full-automatic micropipette, automated cleaning, fluid infusion gas
Colloidal sol Acquisition Instrument automatic sample micropipette device.
Background technique
The disease majority that indoor environment is propagated is related to bioaerosol, and common indoor biological Aerosol Pollution source includes
The sneezing behavior of human and animal, and from outdoor air pollution, these pollution sources can produce respiratory pathogen, dirt
The biological particle such as mite and its breakdown products, mould, fungal spore, mycelia and mycotoxin, these particulate matters can pass through
Respiratory tract enters human body and generates the reaction such as infection, allergy.
In addition to bioaerosols such as fungi, bacteriums common in above-mentioned indoor and outdoor environment, cross-border Introduced cases is new in recent years
Hair burst epidemic constantly occurs, and some highly pathogenic a wide range of propagation with virus, the bacterium of high lethality rate etc. cause
A series of global public health security events.As SARS, H1N1 influenza, the infection of MERS novel coronavirus, H7N9 influenza,
H5N1 bird flu etc..Environment special pathogen monitoring object is generally taken straight mainly based on educable bacterium, fungi at present
Connect the complicated approach such as the infection sources (secretion, excreta, article surface swab etc.) found in environment, completely it is inadaptable currently with
The various cross-border pathogen that aerosol form is sent out.Existing domestic and international commercialization bioaerosol Acquisition Instrument can be divided into impacting type,
Impact type, Filter film type etc. [1], basic principle are all to be collected into microbe granular in solid dielectric by high velocity impact, micro- life
Composition granule can be such that its activity substantially reduces in the collision of fluid field high speed, seriously affect subsequent detection experiment.Simultaneously because centrifugation
Power effect, the lesser virus type aerosol of particle are easy to be thrown away by the air-flow of high speed, seriously affect collecting efficiency, lead to viral gas
Monitoring in terms of colloidal sol is restricted.
It is molten for the mankind and pathogenicity respiratory pathogen (such as H1N1 influenza virus, H7N9 avian influenza virus) gas
Glue particle, most efficient method are to collect the causal organism aerosol particle in air in liquid, and novel pin is to little particle
Virus type sampler has become one of the field current research hot spot.China Inst. of Quarantine Inspection Sciences has now been developed disease
Poison gas colloidal sol acquires enriching instrument, and principle is the Cyclonic aerosol acquisition system based on biphase gas and liquid flow, unique tangential
Formula thief hatch can effectively be collected into virus type nanoscale microbial aerosol in buffer, and fast by nano magnetic particle
Speed enrichment carries out subsequent biological detection again.
However, needing that sample is manually carried out micro transfer after existing bioaerosol sampling and having been manually done life
Analyte detection sample-adding cleaning fluid infusion process.Time-consuming for this process, low efficiency, needs are manually real-time on duty.Currently, in the market micro
Liquid relief is applied mostly on nucleic acid automatic work station, and high-volume sample process is commonly available to, although can use plunger pump from
Reservoir is injected in sample bottle;However, it is desirable to manually replace sample bottle;Processing speed is slow, only pipetting processes just need 2 minutes with
On, it is difficult to meet the job requirement that full automatic treatment bioaerosol Acquisition Instrument subsequent samples quickly detect.
Summary of the invention
It is unique that the object of the present invention is to provide a kind of structure novels, easy to use, and can be realized aerosol Acquisition Instrument
Sample is automatically transferred to the automatic sample micropipette device of detection device;The specific technical proposal is:
A kind of aerosol Acquisition Instrument automatic sample micropipette device, including sample bottle, reservoir and micropipette unit lead to
It crosses the micropipette unit and sample is injected into the reservoir from the sample bottle;It is additionally provided with cleaning container, buffer
Container, waste fluid container, sample bottle discharging of waste liquid unit, reservoir discharging of waste liquid unit, sample bottle cleaning solution injection unit and sample
Product bottle buffer injection unit;The sample bottle discharging of waste liquid unit is by discharging of waste liquid in sample bottle to the waste fluid container;Institute
Reservoir discharging of waste liquid unit is stated by discharging of waste liquid in reservoir to the waste fluid container;The sample bottle cleaning solution injection unit
It will be in the cleaning solution injection sample bottle in the soda liquor container;The sample bottle buffer injection unit holds the buffer
In buffer injection sample bottle in device.
Further, the sample bottle discharging of waste liquid unit include sample bottle waste drains pump and with the waste fluid container and with institute
State the pipe of sample bottle connection;The entrance of the sample bottle waste drains pump is connected by the intracavity bottom of hose or hard tube and sample bottle;
The outlet of sample bottle waste drains pump is connect by hose or hard tube with the waste fluid container.
Further, the reservoir discharging of waste liquid unit include reservoir waste drains pump and with the waste fluid container and with institute
State the pipe of reservoir connection;The entrance of the reservoir waste drains pump is connected by the intracavity bottom of hose or hard tube and the reservoir
It connects;The outlet of reservoir waste drains pump is connect by hose or hard tube with the waste fluid container.
Further, the sample bottle cleaning solution injection unit include cleaning liquid pump and with the soda liquor container and with institute
State the pipe of sample bottle connection;The entrance of the cleaning liquid pump is connected by the intracavity bottom of hose or hard tube and the soda liquor container
It connects;The outlet of cleaning liquid pump is connect by hose or hard tube with the sample bottle.
Further, the sample bottle buffer injection unit include buffer solution pump and the buffer container and with it is described
The pipe of sample bottle connection;The entrance of the buffer solution pump is connected by hose or hard tube and the intracavity bottom of the buffer container
It connects;The outlet of buffer solution pump is connect by hose or hard tube with the sample bottle.
Further, the reservoir cleaning solution injection unit include cleaning liquid pump and with the soda liquor container and with institute
State the pipe of reservoir connection;The entrance of the cleaning liquid pump is connected by the intracavity bottom of hose or hard tube and the soda liquor container
It connects;The outlet of cleaning liquid pump is connect by hose or hard tube with the reservoir.
Further, the reservoir buffer injection unit include buffer solution pump and the buffer container and with it is described
The pipe of reservoir connection;The entrance of the buffer solution pump is connected by hose or hard tube and the intracavity bottom of the buffer container
It connects;The outlet of buffer solution pump is connect by hose or hard tube with the reservoir.
Further, the bottom wall of the sample bottle offers through-hole;The through-hole is connected with three-way connector.
Further, the supervisor of the three-way connector connect with the through-hole;Angle of two branch pipes with the supervisor
Between 110 to 130 degree;Branch pipe is connect with the entrance of the entrance of sample waste pump or sample pump by hose or hard tube respectively.
Further, the sample bottle cleaning solution injection unit, reservoir cleaning solution injection unit, the injection of reservoir buffer
Pump in unit, micropipette unit and sample bottle buffer injection unit is all made of peristaltic pump;The injection of sample bottle cleaning solution is single
Member and reservoir cleaning solution injection unit share a cleaning liquid pump, reservoir buffer injection unit and sample bottle buffer note
Enter one buffer solution pump of units shared, sample bottle, cleaning liquid pump, buffer solution pump are pumped with sample and connected by four-way interface.
The present invention is set for the connectivity problem after the sampling of bioaerosol Acquisition Instrument with biological detection equipment by innovation
Meter establishes the fast trace liquid relief for liquid phase microorganism sampler downstream sample, changes liquid, cleaning, fluid infusion system, a full set of process
It can be completed in 45 seconds.This set system can make microbial aerosol capture and detection apparatus is unattended can be more suitable for collecting in large size
Meeting, emphasis stream of people's logistics place etc..Meet the monitoring capability of strategic area antibioterrorism microbial aerosol, and in hospital
The stream of peoples such as infection, animal-breeding field, entry and exit of the port border hall, the vehicles densely carry out full-automatic infectious disease environmental microorganism
The technology of aerosol monitoring needs.
Detailed description of the invention
Fig. 1 is aerosol Acquisition Instrument automatic sample micropipette system structure of device schematic diagram of the present invention;
Fig. 2 is the structural schematic diagram of sample bottle of the present invention.
In figure: 1, sample bottle;1.1, it is responsible for;1.2, the first branch pipe;1.3, the second branch pipe;2, reservoir;3, waste fluid container;
4, buffer container;5, soda liquor container;6, sample pumps;7, reservoir waste drains pump;8, sample bottle waste drains pump;9, buffer solution pump;
10, liquid pump is cleaned;11, four-way interface.
Specific embodiment
The present invention is made a more thorough explanation below with embodiment.The present invention can be presented as a variety of different forms,
It should not be construed as limited to the exemplary embodiments described herein.
The spatially relative terms such as "upper", "lower" " left side " " right side " can be used herein for ease of explanation, for saying
Relationship of the elements or features relative to another elements or features shown in bright figure.It should be understood that in addition in figure
Except the orientation shown, spatial terminology is intended to include the different direction of device in use or operation.For example, if in figure
Device is squeezed, and is stated as being located at other elements or the element of feature "lower" will be located into other elements or feature "upper".Cause
This, exemplary term "lower" may include both upper and lower orientation.Device, which can be positioned in other ways, (to be rotated by 90 ° or is located at
Other orientation), it can be interpreted accordingly used herein of the opposite explanation in space.
Aerosol Acquisition Instrument automatic sample micropipette device in the present embodiment, including sample bottle 1,2 and of reservoir
Sample is injected reservoir 2 by micropipette unit by micropipette unit from sample bottle 1.Micropipette unit includes sample
The pipe that product pump 6 and connect with reservoir 2, and the pipes that sample pump 6 is connect with sample bottle 1.The entrance of sample pump 6 is by hose or firmly
Pipe is connect with the intracavity bottom of sample bottle 1;The outlet of sample pump 6 is connect by hose or hard tube with reservoir 2.Hose or hard tube
It can be inserted into from the opening of sample bottle 1, the bottom of the opening of pipe as close to 1 inner cavity of sample bottle;To reduce remaining liq.?
It can be inserted into from bottom;Equally, bottom of the opening of pipe as close to 1 inner cavity of sample bottle;To reduce remaining liq.In device
It should also include corresponding control unit, control unit can be controlled using intelligent processors such as single-chip microcontroller, DSP, be passed through
The switch of the controls such as relay pump and valve.Design those skilled in the art of control unit are according to the content of specification according to profession
Rudimentary knowledge carries out, and does not need creative work, is no longer discussed in detail herein.
In order to avoid replacing sample bottle 1;By the way that waste liquid, cleaning and injection buffer is discharged to sample bottle 1, make sample bottle 1
It can reuse;No replacement is required, and that consumptive material achievees the purpose that is unattended.It is also provided with the sample for discharging waste liquid in sample bottle 1
Product bottle discharging of waste liquid unit;For discharging the reservoir discharging of waste liquid unit of waste liquid in reservoir 2;For cleaning sample bottle 1
Sample bottle cleaning solution injection unit;And the sample bottle buffer injection unit for injecting buffer into sample bottle 1.Sample
Bottle discharging of waste liquid unit is by discharging of waste liquid in sample bottle 1 to waste fluid container 3;Reservoir discharging of waste liquid unit will give up in reservoir 2
Liquid is emitted into waste fluid container 3;Cleaning solution in soda liquor container 5 is injected sample bottle 1 by the sample bottle cleaning solution injection unit
It is interior;Sample bottle buffer injection unit will be in the buffer injection sample bottle 1 in buffer container 4.
Sample bottle discharging of waste liquid unit includes the pipeline that sample bottle waste drains pump 8 is connected with 8 both ends of sample bottle waste drains pump;Sample
The entrance of bottle waste drains pump 8 is connect by hose or hard tube with the intracavity bottom of sample bottle 1;The outlet of sample bottle waste drains pump 8 passes through
Hose or hard tube are connect with waste fluid container 3.
Reservoir discharging of waste liquid unit includes the pipeline that reservoir waste drains pump 7 is connected with 7 both ends of reservoir waste drains pump;Liquid storage
The entrance of slot waste drains pump 7 is connect by hose or hard tube with the intracavity bottom of reservoir 2;The outlet of reservoir waste drains pump 7 passes through
Hose or hard tube are connect with waste fluid container 3.
Sample bottle cleaning solution injection unit includes the pipeline for cleaning liquid pump 10 and connecting with cleaning 10 both ends of liquid pump;Clean liquid pump
10 entrance is connect by hose or hard tube with the intracavity bottom of soda liquor container 5;Clean the outlet of liquid pump 10 by hose or
Hard tube is connect with sample bottle 1.Cleaning solution can select medicinal alcohol.
Sample bottle buffer injection unit includes the pipeline that buffer solution pump 9 is connected with 9 both ends of buffer solution pump;Buffer solution pump 9
Entrance connect by hose or hard tube with the intracavity bottom of buffer container 4;The outlet of buffer solution pump 9 passes through hose or hard tube
It is connect with sample bottle 1.
Reservoir cleaning solution injection unit includes the pipeline for cleaning liquid pump 10 and connecting with cleaning 10 both ends of liquid pump;Clean liquid pump
10 entrance is connect by hose or hard tube with the intracavity bottom of soda liquor container 5;Clean the outlet of liquid pump 10 by hose or
Hard tube is connect with reservoir 2.Cleaning solution can select medicinal alcohol.
Reservoir buffer injection unit includes the pipeline that buffer solution pump 9 is connected with 9 both ends of buffer solution pump;Buffer solution pump 9
Entrance connect by hose or hard tube with the intracavity bottom of buffer container 4;The outlet of buffer solution pump 9 passes through hose or hard tube
It is connect with reservoir 2.
Sample bottle discharging of waste liquid and reservoir discharging of waste liquid can share a waste fluid container;Also it can be set individually waste liquid
Container.In order to simplify pipeline, micropipette unit, sample bottle cleaning solution injection unit and sample bottle buffer injection unit three
Unit can be such that the selection of sample bottle 1 connects with a pump in sample pump 6, buffer solution pump 9 or cleaning liquid pump 10 by valve transfer
It connects.Only have a root canal to connect with switching valve;The other end of switching valve is exported with the entrance of sample pump 6 or buffer solution pump 9 respectively,
Or the outlet of cleaning liquid pump 10 passes through piping connection.Switching valve can be three-position four-way valve;Those skilled in the art can choose it
The switching valve of his mode carries out the switching of pipeline.
Buffer solution pump 9, cleaning liquid pump 10, reservoir waste drains pump 7, sample bottle waste drains pump 8 and sample pump 6 can use compacted
Dynamic pump or plunger pump.Peristaltic pump or plunger pump should be equipped with corresponding driver;Peristaltic pump or plunger pump are adopted by control unit
Carry out the control prior art.
As shown in Figure 1, buffer solution pump 9, cleaning liquid pump 10, reservoir waste drains pump 7, sample bottle waste drains pump 8 and sample pump 6 are
When using peristaltic pump, sample bottle 1, buffer solution pump 9, cleaning liquid pump 10 and sample pump 6 can be linked together with four-way interface.
Due to peristaltic pump connecting relative to switch valve and pump, there is cut-off, open and start three kinds of states;End and open two kinds of shapes
When state, switch valve can be used as;As pump when starting;The control of route can be made simpler.
Micropipette unit includes the pipeline that sample bottle 1 is connect with four-way interface 11, four-way interface 11, sample pump in Fig. 1
6;Start sample pump 6, the sample in sample bottle 1 can be injected into dashpot 2 by micropipette unit.
Sample bottle cleaning solution injection unit includes the pipeline for cleaning liquid pump 10 and connecting with cleaning 10 both ends of liquid pump;Clean liquid pump
10 entrance is connect by hose or hard tube with the intracavity bottom of soda liquor container 5;Clean the outlet of liquid pump 10 by hose or
Hard tube is connect by four-way interface 11 with sample bottle 1.
Sample bottle buffer injection unit includes the pipeline that buffer solution pump 9 is connected with 9 both ends of buffer solution pump;Buffer solution pump 9
Entrance connect by hose or hard tube with the intracavity bottom of buffer container 4;The outlet of buffer solution pump 9 passes through hose or hard tube
It is connect by four-way interface 11 with sample bottle 1.
Reservoir cleaning solution injection unit includes the pipeline for cleaning liquid pump 10 and connecting with cleaning 10 both ends of liquid pump;Clean liquid pump
10 entrance is connect by hose or hard tube with the intracavity bottom of soda liquor container 5;Clean the outlet of liquid pump 10 by hose or
Hard tube is connect by four-way interface 11 with reservoir 2.
Reservoir buffer injection unit includes the pipeline that buffer solution pump 9 is connected with 9 both ends of buffer solution pump;Buffer solution pump 9
Entrance connect by hose or hard tube with the intracavity bottom of buffer container 4;The outlet of buffer solution pump 9 passes through hose or hard tube
It is connect by four-way interface 11 with reservoir 2.
System above can also have other deformations, and sample is such as pumped 6 reconfigurations between sample bottle 1 and four-way interface 11;Sample
Product pump 6 is connect with reservoir 2 by four-way interface 11.
As shown in Fig. 2, the bottom wall of sample bottle 1 offers through-hole;Through-hole is connected with three-way connector.
The supervisor 1.1 of three-way connector connect with through-hole;Two branch pipe the first branch pipes 1.2 and the second branch pipe 1.3 and supervisor
1.1 angle is between 110 degree to 130 degree;The entrance of first branch pipe 1.2 and sample bottle waste drains pump 8 passes through hose or hard tube
Connection;Second branch pipe 1.3 is connect with the entrance of sample pump 6 by hose or hard tube;When using four-way interface 11, the second branch pipe
1.3 should connect with a connector in four-way interface 11.
Waste fluid container 3, soda liquor container 5 and buffer container 4 can be the containers such as tank, bottle, pond or slot.
When use, operate as follows:
1) switching valve is switched to sample bottle 1 and connects sample pump 6 by control system;End waste drains pump 8, buffer solution pump 9 and cleaning solution
Pump 10;Sample pump 6 is opened, is extracted sample 5ml out from sample bottle 1 by sample pump 6, injects reservoir 2 through four-way interface 11;
For the processing of micropipette equipment (such as full-automatic nucleic acid work station) liquid relief.
After 2a) sample exhausts, cutoff sample pump 6;Open sample bottle waste drains pump 8 and timing;By the raffinate in sample bottle 1
Body is discharged into waste fluid container 3 as waste liquid;Emptying time can hold according to the flow of sample bottle waste drains pump 8 and the sample of sample bottle 1
Meter calculates;Reach emptying time, closes sample bottle waste drains pump 8.It can also be more than sample capacity by the volume controlled of discharge
Cutoff sample bottle waste drains pump 8 afterwards.
It 3a) opens cleaning liquid pump 10 to extract cleaning solution from soda liquor container 5 out, injects sample bottle 1 through four-way interface 11;
After the capacity (such as 30ml) for reaching setting, cut-off cleaning liquid pump 10.
4a) open sample bottle waste drains pump 8;Waste fluid container 3 is discharged into using the remaining liq in sample bottle 1 as waste liquid;Emptying
Cutoff sample bottle waste drains pump 8 afterwards.
It 5a) opens buffer solution pump 9 to extract buffer from buffer container 4 out, injects sample bottle 1 through four-way interface 11;
Clear up the residual cleaning liquid in sample bottle 1 and pipeline;After the capacity (such as 100ml) for reaching setting, end buffer solution pump 9.
6a) open sample bottle waste drains pump 8;Waste fluid container 3 is discharged into using the remaining liq in sample bottle 1 as waste liquid;Emptying
Cutoff sample bottle waste drains pump 8 afterwards.
It 7a) opens buffer solution pump 9 to extract buffer from buffer container 4 out, injects sample bottle 1 through four-way interface 11;
It prepares for injection next time sample;After the capacity (such as 20ml) for reaching setting, end buffer solution pump 9.
The step of another way are as follows:
After 2b) liquid-transfering gun takes liquid, reservoir waste drains pump 7 is opened, extracts out, is discharged into useless using the remaining liq of reservoir 2 as waste liquid
Liquid container 3;End reservoir waste drains pump 7 after emptying.
3b) open cleaning liquid pump 10, open sample pump 6 or open cleaning liquid pump 10, open sample pump 6 by cleaning solution from
It is extracted out in soda liquor container 5, injects reservoir 2 through four-way interface 11;After reaching setting capacity (such as 100ml), end cleaning solution
Pump 10 and sample pump 6.
4b) open reservoir waste drains pump 7;Waste fluid container 3 is discharged into using the remaining liq in reservoir 2 as waste liquid;Emptying
End reservoir waste drains pump 7 afterwards.
Buffer solution pump 9 5b) is opened, sample pump 6 is opened or opens buffer solution pump 9, opening sample pump 6 postpones buffer
It is extracted out in fliud flushing container 4, injects reservoir 2 through four-way interface 11;Clear up the residual cleaning liquid in reservoir 2 and pipeline;Reach
After setting capacity (such as 100ml), end buffer solution pump 9 and sample pump 6.
6b) open reservoir waste drains pump 7;Waste fluid container 3 is discharged into using the remaining liq in reservoir 2 as waste liquid;Emptying
End reservoir waste drains pump 7 afterwards.
Wherein, 2a) to 7a) and 2b) can be alternately to 6b).Such as: 2b 2a) is executed afterwards);Then 3a)-
3b)……;It is of course also possible to first carry out 2a) to 7a);Then 2b is being executed) to 6b).
The present invention is set for the connectivity problem after the sampling of bioaerosol Acquisition Instrument with biological detection equipment by innovation
Meter establishes the fast trace liquid relief for liquid phase microorganism sampler downstream sample, changes liquid, cleaning, fluid infusion system, a full set of process
It can be completed in 45 seconds.This set system can make microbial aerosol capture and detection apparatus is unattended can be more suitable for collecting in large size
Meeting, emphasis stream of people's logistics place etc..Meet the monitoring capability of strategic area antibioterrorism microbial aerosol, and in hospital
The stream of peoples such as infection, animal-breeding field, entry and exit of the port border hall, the vehicles densely carry out full-automatic infectious disease environmental microorganism
The technology of aerosol monitoring needs.
Above-mentioned example is only intended to illustrate the present invention, and in addition to this, also there are many different embodiments, and these are implemented
Mode be all those skilled in the art after comprehension inventive concept it is also envisioned that therefore, will not enumerate herein.
Claims (10)
1. a kind of aerosol Acquisition Instrument automatic sample micropipette device, including sample bottle, reservoir and micropipette unit,
Sample is injected into the reservoir from the sample bottle by the micropipette unit;It is characterized in that, being additionally provided with clear
Wash container, buffer container, waste fluid container, sample bottle discharging of waste liquid unit, reservoir discharging of waste liquid unit, sample bottle cleaning solution note
Enter unit, reservoir cleaning solution injection unit, reservoir buffer injection unit and sample bottle buffer injection unit;The sample
Product bottle discharging of waste liquid unit is by discharging of waste liquid in sample bottle to the waste fluid container;The reservoir discharging of waste liquid unit is by liquid storage
Discharging of waste liquid is to the waste fluid container in slot;The sample bottle cleaning solution injection unit is by the cleaning solution in the soda liquor container
It injects in sample bottle;The sample bottle buffer injection unit will be in the buffer injection sample bottle in the buffer container;
The reservoir cleaning solution injection unit will be in the cleaning solution injection reservoir in the soda liquor container;The reservoir buffering
Liquid injection unit will be in the buffer injection reservoir in the buffer container.
2. aerosol Acquisition Instrument automatic sample micropipette device as described in claim 1, which is characterized in that the sample
Bottle discharging of waste liquid unit include sample bottle waste drains pump and with the waste fluid container and the pipe being connect with the sample bottle;The sample
The entrance of product bottle waste drains pump is connected by the intracavity bottom of hose or hard tube and sample bottle;The outlet of sample bottle waste drains pump passes through soft
Pipe or hard tube are connect with the waste fluid container.
3. aerosol Acquisition Instrument automatic sample micropipette device as described in claim 1, which is characterized in that the liquid storage
Slot discharging of waste liquid unit include reservoir waste drains pump and with the waste fluid container and the pipe being connect with the reservoir;The storage
The entrance of liquid bath waste drains pump is connect by hose or hard tube with the intracavity bottom of the reservoir;The outlet of reservoir waste drains pump is logical
Hose or hard tube is crossed to connect with the waste fluid container.
4. aerosol Acquisition Instrument automatic sample micropipette device as described in claim 1, which is characterized in that the sample
Bottle cleaning solution injection unit include cleaning liquid pump and with the soda liquor container and the pipe being connect with the sample bottle;It is described clear
The entrance of lotion pumps is connect by hose or hard tube with the intracavity bottom of the soda liquor container;The outlet of cleaning liquid pump passes through soft
Pipe or hard tube are connect with the sample bottle.
5. aerosol Acquisition Instrument automatic sample micropipette device as described in claim 1, which is characterized in that the sample
Bottle buffer injection unit includes buffer solution pump and the buffer container and the pipe connecting with the sample bottle;The buffering
The entrance of liquid pump is connect by hose or hard tube with the intracavity bottom of the buffer container;The outlet of buffer solution pump passes through hose
Or hard tube is connect with the sample bottle.
6. aerosol Acquisition Instrument automatic sample micropipette device as described in claim 1, which is characterized in that the liquid storage
Slot cleaning solution injection unit include cleaning liquid pump and with the soda liquor container and the pipe being connect with the reservoir;It is described clear
The entrance of lotion pumps is connect by hose or hard tube with the intracavity bottom of the soda liquor container;The outlet of cleaning liquid pump passes through soft
Pipe or hard tube are connect with the reservoir.
7. aerosol Acquisition Instrument automatic sample micropipette device as described in claim 1, which is characterized in that the liquid storage
Slot buffer injection unit includes buffer solution pump and the buffer container and the pipe connecting with the reservoir;The buffering
The entrance of liquid pump is connect by hose or hard tube with the intracavity bottom of the buffer container;The outlet of buffer solution pump passes through hose
Or hard tube is connect with the reservoir.
8. aerosol Acquisition Instrument automatic sample micropipette device as described in claim 1, which is characterized in that the sample
The bottom wall of bottle offers through-hole;The through-hole is connected with three-way connector.
9. aerosol Acquisition Instrument automatic sample micropipette device as claimed in claim 8, which is characterized in that the threeway
The supervisor of connector connect with the through-hole;Two branch pipes are between the angle 110 to 130 of supervisor degree;Branch pipe difference
It is connect with the entrance of the entrance of sample waste pump or sample pump by hose or hard tube.
10. aerosol Acquisition Instrument automatic sample micropipette device as described in claim 1, which is characterized in that the sample
Product bottle cleaning solution injection unit, reservoir cleaning solution injection unit, reservoir buffer injection unit, micropipette unit and sample
Pump in product bottle buffer injection unit is all made of peristaltic pump;Sample bottle cleaning solution injection unit and the injection of reservoir cleaning solution are single
Member shares a cleaning liquid pump, and reservoir buffer injection unit and sample bottle buffer injection unit share a buffer
Pump, sample bottle, cleaning liquid pump, buffer solution pump are pumped with sample and are connected by four-way interface.
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