CN1994100A - A formulated milk-contained drink and method for preparing same - Google Patents
A formulated milk-contained drink and method for preparing same Download PDFInfo
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Abstract
The invention relates to a method for preparing milk drink, wherein it comprises 30-60g milk, 0.01-10.0g isomaltose hypgather, 4. 0-12. 0g sugar, 0.1-1. 0g stabilizer, 0.2-0.7g acid adjuster, 0.05-0.4g flavouring essence, and the left is water. Its production comprises that: 1, preparing materials; 2, dissolving sugar and stabilizer into water to be mixed with milk, adding acid adjuster, to be mixed uniformly; 3, preheating into 25-80Deg. C, preparing uniformly at 15-25Mpa; 4, adding flavouring essence, mixing and disinfecting.
Description
Technical field
The present invention relates to beverage and preparation method thereof, particularly relate to a kind of blending type milk-contained drink and preparation method thereof.
Background technology
Blending type milk-contained drink in the market is to be raw material with sweet milk or dairy products, adds that modulation such as entry, liquid glucose, acid form.The beverage that protein content is not less than 1.0% (m/V) in the finished product is called milk beverage; Protein content is not less than 0.7% beverage and is called lactic acid drink (GB10789-1996).The main component of blending type milk-contained drink is water, milk, white granulated sugar, thickener, emulsifying agent, acidity regulator and flavorant, according to the requirement of people to health, can also in product, add nutriments (Xie Jizhi writes, the liquid diary product science and technology) such as vitamin, mineral matter, amino acid or fruit juice.Such beverage developed very fast in recent years, the growth rate of annual demand is all more than 25.0%, particularly high-temperature short-time sterilization and aseptic packaging are after using on the blending type milk-contained drink, the nutritional labeling that has not only farthest kept cow's milk, the shelf-life of product was reached more than six months, enlarged the sales range of product, thus make the blending type milk-contained drink with its multiple tastes, be of high nutritive value and drink characteristics such as convenient, in milk beverage market, occupied leading position.
The propagation of Bifidobacterium has benifit to the National People's Congress in the human body, yet the quantity of Bifidobacterium is still a technical barrier up to now in the raising human body.This is because Bifidobacterium is the anaerobic type flora, in air, be difficult to survival, the acidity of adding hydrochloric acid in gastric juice is very strong, the overwhelming majority all has been killed during Bifidobacterium process stomach, have only few part to arrive in the intestines, thereby make its beneficial functional be difficult to embody, and the flora of the Bifidobacterium of different people is also incomplete same human body, promptly allow to replenish the Bifidobacterium that lives, can not adapt to all crowds.
Oligoisomaltose (IMO) is a kind of of starch sugar, and main component is by α-1, the isomaltose (IG of 6-glycosidic bond combination
2), panose (P), Isomaltotriose (IG
3) and the above (G of tetrose
N) functional oligose that is combined into.Can be divided into two kinds in oligoisomaltose slurry and oligoisomaltose powder by form.Syrup is colourless or light yellow, transparent thick liquid, and sweet taste is soft, free from extraneous odour, the visible impurity of no twenty-twenty vision.Icing Sugar is white amorphous powder, and sweet taste is soft, free from extraneous odour, the visible impurity of no twenty-twenty vision.The basic composition of different purity oligoisomaltose (IMO) is as shown in table 1.The production technology of oligoisomaltose (IMO) is roughly: the exquisite cornstarch-liquefaction-saccharification-enzyme that the goes out-essence filter-commentaries on classics glycosides-enzyme that goes out-essence filter (micro-filtration)-ion-exchange-concentrate-finished product.
The basic composition of table 1 different purity oligoisomaltose (IMO)
| Project | Requirement | |
| The IMO-50 type | The IMO-90 type | |
| Syrup | Icing Sugar | Syrup | Icing Sugar | |
| IMO content (accounting for dry) % 〉= | 50 | 90 | ||
| IG 2+P+IG 3Content (accounting for dry) % 〉= | 35 | 45 | ||
| Dry (solid content)/% 〉= | 75 | - | 75 | - |
| Moisture/%≤ | - | 5 | - | 5 |
| pH | 4.0-6.0 | |||
| Transmittance/% 〉= | 95 | - | 95 | - |
| Solubility/% 〉= | - | 99 | - | 99 |
| Sulfated ass/%≤ | 0.3 | |||
| Arsenic/(mg/kg)≤ | 0.5 | |||
| Plumbous/(mg/kg)≤ | 0.5 | |||
| Total plate count/[cfu/ (g or mL)]≤ | 1500 | |||
| Coliform/[MPN/ (100g or 100mL)]≤ | 30 | |||
| Pathogenic bacteria (salmonella) | Must not detect | |||
Summary of the invention
The purpose of this invention is to provide a kind of oligoisomaltose (IMO) that contains, can promote the blending type milk-contained drink of beneficial bacterium in the body-Bifidobacterium propagation.
Blending type milk-contained drink provided by the invention, its prescription comprise following component (every 100g):
Milk 30-60g,
Oligoisomaltose (IMO) 0.01-10.0g,
Sugar 4.0-12.0g,
Stabilizing agent 0.1-1.0g,
Acidity regulator 0.2-0.7g,
Flavoring essence 0.01-0.4g,
Add water to 100g.
The addition of oligoisomaltose is that oligoisomaltose (IMO) with 50% purity is a standard metering in the above-mentioned blending type milk-contained drink prescription, i.e. IG
2+ P+IG
3〉=35%, sugariness is the 40-50% of sucrose, pure taste, similar sucrose.The oligoisomaltose of other purity can be converted according to the difference of content.
Described stabilizing agent is emulsifying agent or thickener, or is combined by emulsifying agent and thickener, and wherein, the ratio of weight and number of emulsifying agent and thickener is 1: 1-60; Described emulsifying agent can be selected from one or more the combination in sucrose fatty ester, fatty acid glyceride and the polyglycerol ester; Thickener can be selected from one or more the combination in sodium carboxymethylcellulose, carragheen, gelatin, microcrystalline cellulose, gellan gum, xanthans, propylene glycol alginate, konjac glucomannan, agar, locust bean gum and the pectin.
Described acidity regulator can be selected from one or more the combination in lactic acid, citric acid, phosphoric acid, tartaric acid and the malic acid.
Described flavoring essence can be selected from sour milk essence, milk flavour, orange essence, grape essence, peach essence, cocoanut flavour, strawberry essence, lychee flavor, flavoring apple essence, close melon essence, mango essence, passionflower essence, grapefruit essence, lemon extract, blueberry essence, pawpaw essence, flavoring banana essence, flavoring pineapple essence, tomato flavour, carrot essence, asparagus essence, spinach essence, apricot essence, pear essence, kiwi flavor, vanilla, flavoring rose essence, chocolate essence, walnut essence, the combination of one or more in wheat essence and the cherry essence.
Described sugar can be any one or a few the mixing in white granulated sugar, syrup and the sweetener, and conversion is the white granulated sugar pol when calculating pol.
Described milk is defined as: common cow's milk comprises full-cream, degreasing and half degreasing; Or with the formulated recombined milk of other component of milk powder, cream, protein hydrolysate, whey powder or milk.
For nutritive value and the local flavor taste that improves blending type milk-contained drink of the present invention, can add 0.5-20g Juice or fruit-vegetable granules again in its prescription, and/or the 0.01-0.5g trace element, and/or the 0.3-0.8g dietary fiber.
Described fruit-vegetable granules can be selected from the really combination of one or more in the various fruit-vegetable granules such as grain, celery particle, the operatic circle grain, cucumber particle, radish kind particle and aloe gel particle of tomato particle, peach particle, coconut palm fruit granule, orange fruit grain, mango fruit grain, grape fruit, pawpaw fruit grain, pineapple fruit grain, strawberry; Usually select granular garden stuff grain or the slurry class fruit and vegetable of diameter 1-20mm for use, or length is that 1-20mm, width are the fibrous fruit and vegetable of 1-10mm.
Described Juice can be selected from one or more the combination in the various Juices such as cider, strawberry juice, blueberry juice, Chinese flowering quince juice, bananas juice, orange juice, pineapple juice, mango juice, tomato juice, carrot juice, asparagus juice, spinach juice, peach juice, apricot juice, pear juice, grape juice, asparagus juice, kiwi-fruit juice, Lychee juice, lemon juice and Coconut Juice.
Trace element can be selected from vitamin A, vitamin B complex, vitamin D, vitamin C, vitamin E, calcium lactate, taurine, folic acid, nicotinic acid, choline, ferrous sulfate, ironic citrate, zinc sulfate, zinc gluconate, sodium selenite, magnesium sulfate, magnesium gluconate and l-cn etc. to one or more the combination in human body beneficial's the trace element.
Second purpose of the present invention provides a kind of preparation method of above-mentioned blending type milk-contained drink.
Preparation method provided by the present invention can may further comprise the steps:
1) get material (every 100g) by following composition of raw materials: milk 30-60g, oligoisomaltose (IMO) 0.01-10.0g, sugared 4.0-12.0g, stabilizing agent 0.1-1.0g, acidity regulator 0.2-0.7g, flavoring essence 0.01-0.4g adds water to 100g;
2) oligoisomaltose (IMO), sugar and stabilizing agent water dissolving back are fully mixed with milk, under stirring condition, add acidity regulator again, mixing;
3) with step 2) mixed liquor be preheated to 25-80 ℃, under pressure 13-40Mpa, it is carried out homogeneous again;
4) add flavoring essence, stir, sterilization obtains the blending type milk-contained drink.
In the preparation method of blending type milk-contained drink of the present invention, the addition of the oligoisomaltose in the raw material (IMO) is that oligoisomaltose (IMO) with 50% purity is a standard metering, i.e. IG
2+ P+IG
3〉=35%, sugariness is the 40-50% of sucrose, pure taste, similar sucrose.The oligoisomaltose of other purity (IMO) can be converted according to the difference of content.Described oligoisomaltose (IMO) can be made by oneself or buy from the market, as become the oligoisomaltose (IMO) of companies such as food Development Co., Ltd well available from Shandong bowling treasured, Zhengzhou Fu Run moral bioengineering Co., Ltd or Shanghai.
Stabilizing agent in the raw material is emulsifying agent or thickener, or is combined by emulsifying agent and thickener, and wherein, the ratio of weight and number of emulsifying agent and thickener is 1: 1-60; Described emulsifying agent can be selected from one or more the combination in sucrose fatty ester, fatty acid glyceride and the polyglycerol ester; Thickener can be selected from one or more the combination in sodium carboxymethylcellulose, carragheen, gelatin, microcrystalline cellulose, gellan gum, xanthans, propylene glycol alginate, konjac glucomannan, agar, locust bean gum and the pectin.
Acidity regulator in the raw material can be selected from one or more the combination in lactic acid, citric acid, phosphoric acid, tartaric acid and the malic acid.
Flavoring essence in the raw material can be selected from sour milk essence, milk flavour, orange essence, grape essence, peach essence, cocoanut flavour, strawberry essence, lychee flavor, flavoring apple essence, close melon essence, mango essence, passionflower essence, grapefruit essence, lemon extract, blueberry essence, pawpaw essence, flavoring banana essence, flavoring pineapple essence, tomato flavour, carrot essence, asparagus essence, spinach essence, apricot essence, pear essence, kiwi flavor, vanilla, flavoring rose essence, chocolate essence, walnut essence, the combination of one or more in wheat essence and the cherry essence.
Sugar in the raw material can be any one or a few the mixing in white granulated sugar, syrup or the sweetener, and conversion is the white granulated sugar pol when calculating pol.
Milk is defined as in the raw material: common cow's milk comprises full-cream, degreasing and half degreasing; Or with the formulated recombined milk of other components of milk powder, cream, protein hydrolysate, whey powder or milk.
The condition of sterilizing in the step 4) was preferably: 90-140 ℃ of following sterilization 4 seconds to 15 minutes.
Nutritive value and local flavor taste in order to improve blending type milk-contained drink of the present invention can add 0.5-20g Juice and/or fruit-vegetable granules again in its preparation process, and/or the 0.01-0.5g trace element, and/or the 0.3-0.8g dietary fiber; Described Juice, trace element and/or dietary fiber are in step 2) in add, after the adding again with the acidity regulator mixing; Fruit-vegetable granules adds in step 4), sterilization again after the adding.
Described fruit-vegetable granules can be selected from the really combination of one or more in the various fruit-vegetable granules such as grain, celery particle, the operatic circle grain, cucumber particle, radish kind particle and aloe gel particle of tomato particle, peach particle, coconut palm fruit granule, orange fruit grain, mango fruit grain, grape fruit, pawpaw fruit grain, pineapple fruit grain, strawberry; Usually select granular garden stuff grain or the slurry class fruit and vegetable of diameter 1-20mm for use, or length is that 1-20mm, width are the fibrous fruit and vegetable of 1-10mm.
Described Juice can be selected from one or more the combination in the various Juices such as cider, strawberry juice, blueberry juice, Chinese flowering quince juice, bananas juice, orange juice, pineapple juice, mango juice, tomato juice, carrot juice, asparagus juice, spinach juice, peach juice, apricot juice, pear juice, grape juice, asparagus juice, kiwi-fruit juice, Lychee juice, lemon juice and Coconut Juice.
Trace element can be selected from vitamin A, vitamin B complex, vitamin D, vitamin C, vitamin E, calcium lactate, taurine, folic acid, nicotinic acid, choline, ferrous sulfate, ironic citrate, zinc sulfate, zinc gluconate, sodium selenite, magnesium sulfate, magnesium gluconate and l-cn etc. to one or more the combination in human body beneficial's the trace element.
The invention provides a kind of blending type milk-contained drink and preparation method thereof.This blending type milk-contained drink is the milk beverage that has added oligoisomaltose (IMO), compare with existing like product, have the following advantages and useful effect: 1) the various beneficial bacteriums (such as Bifidobacterium, lactic acid bacteria etc.) that contained in oligoisomaltose (IMO) in the beverage of the present invention and milk, the enteron aisle are coordinated mutually, bring out the best in each other, not only improve taste, better the effect of performance profitable strain; 2) can make multiple beneficial bacterium (particularly Bifidobacterium) propagation in the body, and then play and improve human body stomach environment, promote intestines peristalsis, reduce poisonous tunning and harmful bacteria, prevent constipation, the protection liver function, reduce serum cholesterol, bring high blood pressure down, improve body immunity, promote the effect that the B family vitamin is synthetic, help nutrients such as calcium, iron, zinc to absorb.Human experimentation is the result show, the quantity of taking Bifidobacterium in forward and backward each experimental group enteron aisle obviously increases, and amplification can reach 10 times, shows that beverage of the present invention can obviously promote the propagation of Bifidobacterium in the enteron aisle; 3) product structure and the prescription of having broken through original blending type milk-contained drink formed, and can add Juice, fruit and vegetable or trace element as required, makes the blending type milk beverage have more the comprehensive nutrient effect, and makes the product category abundanter; 4) raw material sources are extensive, and the preparation method is simple, are easy to carry out suitability for industrialized production.The present invention will play a significant role at blending type milk-contained drink and the health care food production field of effectively breeding Bifidobacterium, and market prospects are wide.
Below in conjunction with specific embodiment the present invention is described in further details.
Description of drawings
Fig. 1 is the flow sheet of blending type milk-contained drink of the present invention
The specific embodiment
Method therefor is conventional method if no special instructions among the following embodiment.Used degree is the quality percentage composition if no special instructions among the following embodiment.
Embodiment 1, oligoisomaltose (IMO) are regulated the zoopery of gut flora function
Given the test agent: oligoisomaltose (IMO), available from the precious company of Shandong bowling, purity 50% is white powder.Preserve shady and cool, dry, ventilation.
Animal used as test: select the 18-22g available from Institute of Experimental Animals, Chinese Academy of Medical Sciences (credit number: SCXK-(capital) 2004-0001) for use, 48 of the healthy cleaning level of BALB/C male mices are divided into four groups, 12 every group.
Dosage: the recommended amounts of oligoisomaltose (IMO) is adult (pressing the 60kg batheroom scale) 10g every day, 20g and 30g.According to three RDs 10 times are provided with mouse experiment dosage respectively, and promptly every day, dosage was respectively 1.67g/kgBW (low dose group), 3.33g/kgBW (middle dosage group), 5.00g/kgBW (high dose group).Tried thing water (sterilizing) preparation, per os gives mouse once a day and is tried thing, and the continuous irrigation stomach is tested every index after 14 days.The mouse stomach volume is heavy for the 0.10mL/10g mouse.Establish blank group (0g/kgBW) simultaneously, water (sterilizing) replaces being tried thing, and it is long-pending with respectively to be tried the thing group identical to irritate body of stomach every day.
Before giving given the test agent, the aseptic stool in mice number got is put in the aseptic vessel, uses the assay balance weighing, record weight.Then, in clean bench, sterile working adds dilution (redistilled water), is diluted to 10
-2, the mixing that fully vibrates is according to 10 times of serial dilutions to 10
-8, to measure bacterium for every kind and select suitable dilution factor according to a conventional method, inoculation is dull and stereotyped, detects the flora quantity of five kinds of typical bacterium of enteron aisle (Bifidobacterium, lactobacillus, enterococcus, enterobacteria, C.perfringens).Culture medium, cultivation and authentication method are as shown in table 2, calculate the wet clump count (cfu/g) in just of every gram then, except that C.perfringens, all take the logarithm and carry out statistical disposition.Give given the test agent 24h afterwards the last time, detect the flora quantity of five kinds of typical bacterium of above-mentioned enteron aisle once more, method step is the same.
Data are handled: with SPSS software the initial data of each experimental group is carried out data and handle, adopt the program of variance analysis to carry out homogeneity test of variance earlier, variance is neat, calculates the F value, F value＜F
0.05, conclusion: each organizes the mean differences does not have conspicuousness; F value 〉=F
0.05, P≤0.05 is added up with the comparative approach in twos of mean between a plurality of experimental group and control group; The data of Non-Gaussian Distribution or heterogeneity of variance are carried out the conversion of suitable variable, wait to satisfy normal state or variance are neat require after, add up with the data after changing; If still for to reach normal state or the neat purpose of variance, use rank test instead and add up after the variable conversion.
The result judges: last, criterion according to " health food check and assessment technique standard " (version in 2003), Bifidobacterium between comparative experiments front and back self and group, lactobacillus, enterococcus, enterobacteria, the number change situation of C.perfringens, experimental group experiment front and back self comparing difference has conspicuousness, or comparing difference has conspicuousness between experiment back experimental group and control group group, and experimental group experiment front and back self comparing difference has conspicuousness, meet with the next item down, can judge this group given the test agent zoopery positive as a result: Bifidobacterium and/or lactobacillus obviously increase in (1) ight soil, C.perfringens reduces or does not increase enterobacteria, enterococcus does not have significant change.(2) Bifidobacterium and/or lactobacillus obviously increase in the ight soil, and C.perfringens reduces or do not increase, and enterobacteria and/or enterococcus obviously increase, but the amplitude that increases is lower than the amplitude that Bifidobacterium/lactobacillus increases.
Oligoisomaltose (IMO) is as shown in table 3 to the testing result of mouse body weight influence, initial body weight of mouse and experiment back body weight compare in each dosage group and 0g/kgBW group, there are no significant for difference (P＞0.05), shows that oligoisomaltose (IMO) has no adverse effects to the body weight of mouse.
The testing result of enterobacteria flora quantity is as shown in table 4 in the mouse intestinal of experiment front and back, give and tried to compare between preceding each dosage group of thing and 0g/kgBW group, the quantity of enterobacteria does not all have significant difference (P＞0.05), and compared with the 0g/kgBW group after 14 days to trying thing, 5.00g/kgBW the quantity of group enterobacteria reduces, there were significant differences (P＜0.05).Each dosage group self enterobacteria quantity there are no significant difference (P＞0.05) relatively before and after the experiment.
The testing result of enterococcus flora quantity is as shown in table 5 in the mouse intestinal of experiment front and back, give and tried to compare between preceding each dosage group and 0 g/kgBW group, enterococcal quantity there are no significant difference (P＞0.05), compared with 0 g/kgBW group after 14 days to trying thing, reduce 5.00 g/kgBW organizes enterococcal quantity, there were significant differences (P＜0.05).Each dosage group self enterococcus there are no significant difference (P＞0.05) relatively before and after the experiment.
The testing result of lactobacillus flora quantity is as shown in table 6 in the mouse intestinal of experiment front and back, gives and is tried to compare the quantity there was no significant difference of lactobacillus (P＞0.05) between forward and backward each dosage group of thing and 0g/kgBW group.Give and to be tried forward and backward each dosage group of thing self relatively, the quantity of lactobacillus there are no significant difference (P＞0.05).
Before and after the experiment in the mouse intestinal testing result of C.perfringens flora quantity as shown in table 7, give and tried to compare between forward and backward each dosage group and 0g/kgBW group, the quantity of C.perfringens there are no significant difference (P＞0.05).Give and tried respectively to organize self relatively before and after the thing, the quantity of C.perfringens does not all have significant difference (P＞0.05).
Before and after the experiment in the mouse intestinal testing result of Bifidobacterium flora quantity shown in table 8 and table 9, as shown in Table 8, give and tried to compare between forward and backward each dosage group and 0g/kgBW group, the quantity of Bifidobacterium there are no significant difference (P＞0.05).Give tried thing before and after 0g/kgBW, 1.67g/kgBW group through self relatively, the quantity of Bifidobacterium there are no significant difference (P＞0.05); 3.33g/kgBW organize self relatively, the quantity of Bifidobacterium increases, and significant difference (P＜0.05) is arranged; 5.00g/kgBW experimental group self relatively, the quantity of Bifidobacterium increases, and significant difference (P＜0.01) is arranged.As shown in Table 9, give and to be tried comparison between forward and backward each dosage group and 0g/kgBW group, the quantity of Bifidobacterium there are no significant difference (P＞0.05).Give and tried thing front and back 3.33g/kgBW group self relatively, the quantity of Bifidobacterium increases by 1.4 times; 5.00g/kgBW organize self relatively, the quantity of Bifidobacterium increases by 2.0 times.
In sum, per os gives mouse oligoisomaltose (IMO) 14 days front and back, and the 3.33g/kgBW group self compares, and the quantity of Bifidobacterium increases by 1.4 times in the mouse intestines, and significant difference (P＜0.05) is arranged; 5.00g/kgBW organize self relatively, the quantity of Bifidobacterium increases by 2.0 times, and significant difference (P＜0.01) is arranged.Per os gave mouse oligoisomaltose (IMO) after 14 days, compared with the 0g/kgBW group, and 5.00g/kgBW group enterobacteria quantity reduces (P＜0.05); Enterococcus quantity significantly reduces (P＜0.05).Give before and after the oligoisomaltose (IMO), each organizes mouse C.perfringens quantity does not all have marked change.Oligoisomaltose (IMO) has no adverse effects to the mouse body weight.
Above-mentioned experimental result shows that it is positive that oligoisomaltose (IMO) is regulated the gut flora contractile studies, proves that oligoisomaltose (IMO) can the had significant proliferation intestinal bifidobacteria.
The check of table 2 gut flora culture medium, cultivation and authentication method
| The bacterium name | Culture medium | Condition of culture | Authentication method |
| Enterobacteria | Eosin methylene blue agar (Qingdao Hai Bo Bioisystech Co., Ltd) | 36±1℃，24h | Counting lactose fermenters, dyeing microscopic examination are all bacterium colonies of G bacillus |
| Enterococcus | Sodium azide-crystal violet-aesculin agar (Qingdao Hai Bo Bioisystech Co., Ltd) | 36±1℃，48h | It is all bacterium colonies of G coccus that counting has obvious brown circle, dyeing microscopic examination |
| Lactobacillus | Lactobacillus selective medium (Qingdao Hai Bo Bioisystech Co., Ltd) | 36±1℃，48h | According to GB/ T4789.34-2003 |
| Bifidobacterium | Bifidobacterium selective medium (Qingdao Hai Bo Bioisystech Co., Ltd) | Anaerobism is cultivated 36 ± 1 ℃, 48h | G sporeless bacterium, the negative APICH50 of catalase |
| C.perfringens | C.perfringens selective medium (Qingdao Hai Bo Bioisystech Co., Ltd) | Anaerobism is cultivated 36 ± 1 ℃, 24h | Count all have fluorescence under ultraviolet light black bacterium colony |
Respectively organize before and after table 3 experiment mouse body weight (
± SD)
| Group | Number of animals (only) | Body weight (g) before the experiment | With 0g/kgBW group P value relatively | Experiment back body weight (g) | With 0g/kgBW group P value relatively |
| 0g/kgBW | 12 | 21.4±0.9 | 26.6±1.2 | …… | |
| 1.67g/kgBW | 12 | 21.1±1.3 | 0.895 | 25.5±1.7 | 0.156 |
| 3.33g/kgBW | 12 | 21.4±1.1 | 1.000 | 26.3±1.8 | 0.890 |
| 5.00g/kgBW | 12 | 21.4±0.8 | 1.000 | 25.4±1.2 | 0.137 |
The testing result of enterobacteria flora quantity in table 4 mouse intestinal (log10cfu/g,
± SD)
| Group | Number of animals (only) | Give and tried thing | With 0g/kgBW group P value relatively | To after being tried thing | With 0g/kgBW group P value relatively | This group is given the P value of being tried the thing front and back |
| 0g/kgBW | 12 | 5.97±0.27 | …… | 6.32±0.4 | …… | 0.062 |
| 1.67g/kgBW | 12 | 6.14±0.31 | 0.338 | 6.12±0.31 | 0.271 | 0.751 |
| 3.33g/kgBW | 12 | 6.09±0.24 | 0.635 | 6.04±0.27 | 0.087 | 0.587 |
| 5.00g/kgBW | 12 | 6.07±0.33 | 0.763 | 5.96±0.23a | 0.021 | 0.207 |
A: relatively there were significant differences with the 0g/kgBW group
Enterococcus flora quantity testing result in table 5 mouse intestinal (log10cfu/g,
± SD)
| Group | Number of animals (only) | Give and tried thing | With 0g/kgBW group P value relatively | To after being tried thing | With 0g/kgBW group P value relatively | This group is given the P value of being tried the thing front and back |
| 0g/kgBW | 12 | 6.98±0.30 | …… | 7.29±0.61 | …… | 0.068 |
| 1.67g/kgBW | 12 | 7.04±0.38 | 0.974 | 7.11±0.39 | 0.677 | 0.546 |
| 3.33g/kgBW | 12 | 6.82±0.53 | 0.669 | 6.84±0.47 | 0.065 | 0.828 |
| 5.00g/kgBW | 12 | 8.92±0.50 | 0.971 | 6.74±0.40a | 0.019 | 0.292 |
A: relatively there were significant differences with the 0g/kgBW group
Table 6 mouse lactobacillus flora quantity testing result (log10cfu/g,
± SD)
| Group | Number of animals (only) | Give and tried thing | With 0g/kgBW group P value relatively | To after being tried thing | With 0g/kgBW group P value relatively | This group is given the P value of being tried the thing front and back |
| 0g/kgBW | 12 | 8.30±0.29 | …… | 8.39±0.54 | 0.559 | |
| 1.67g/kgBW | 12 | 8.31±0.19 | 1.000 | 8.41±0.39 | 0.999 | 0.313 |
| 3.33g/kgBW | 12 | 8.29±0.25 | 1.000 | 8.42±0.25 | 0.998 | 0.128 |
| 5.00g/kgBW | 12 | 8.28±0.50 | 0.998 | 8.56±0.30 | 0.605 | 0.120 |
Table 7 mouse C.perfringens flora quantity testing result (cfu/g,
± SD)
| Group | Number of animals (only) | To before being tried thing | With 0g/kgBW group P value relatively | To after being tried thing | With 0g/kgBW group P value relatively | This group is given the P value of being tried the thing front and back |
| 0g/kgBW | 12 | 17±25 | …… | 21±26 | …… | 0.723 |
| 1.67g/kgBW | 12 | 17±33 | 1.000 | 21±26 | 1.000 | 0.674 |
| 3.33g/kgBW | 12 | 17±23 | 0.970 | 12±23 | 0.736 | 1.000 |
| 5.00g/kgBW | 12 | 17±33 | 1.000 | 12±23 | 0.736 | 0.586 |
Bifidobacterium flora quantity testing result in table 8 mouse intestinal (log10cfu/g,
± SD)
| Group | Number of animals (only) | Give and tried thing | With 0g/kgBW group P value relatively | To after being tried thing | With 0g/kgBW group P value relatively | This group is given the P value of being tried the thing front and back |
| 0g/kgBW | 12 | 8.60±0.23 | …… | 8.77±0.36 | …… | 0.165 |
| 1.67g/kgBW | 12 | 8.59±0.36 | 1.000 | 8.81±0.36 | 0.984 | 0.062 |
| 3.33g/kgBW | 12 | 8.55±0.30 | 0.970 | 8.88±0.40b | 0.805 | 0.040 |
| 5.00g/kgBW | 12 | 8.65±0.36 | 0.956 | 9.11±0.35b | 0.069 | 0.001 |
B: relatively there were significant differences before being tried thing with this group
Bifidobacterium flora quantity testing result (* 10 in table 9 mouse intestinal
8Cfu/g,
± SD)
| Group | Number of animals (only) | To before being tried thing | To after being tried thing | This group is given and is tried relatively to increase before and after the thing |
| 0g/kgBW | 12 | 4.4±1.9 | 7.5±5.2 | —— |
| 1.67g/kgBW | 12 | 5.4±4.7 | 8.3±5.3 | —— |
| 3.33g/kgBW | 12 | 4.5±3.9 | 10.8±9.4 | 1.4 doubly |
| 5.00g/kgBW | 12 | 5.5±2.9 | 16.5±10.6 | 2.0 doubly |
Embodiment 2, oligoisomaltose (IMO) are regulated the human experimentation of gut flora function
Given the test agent: oligoisomaltose (IMO), available from the precious company of Shandong bowling, purity 50% is white powder.Preserve shady and cool, dry, ventilation.
Experimenter group: through 60 of all normal adults of clinical health check-up index, the men and women half and half.The experimenter middle part is divided into constipation person.
The enteric bacteria cultural method is as follows:
Bifidobacterium: BBL agar medium (Qingdao Hai Bo Bioisystech Co., Ltd), 37 ℃, anaerobism was cultivated in 48-72 hour;
Lactobacillus: LBs agar medium (Qingdao Hai Bo Bioisystech Co., Ltd), 37 ℃, cultivated in 48 hours;
Enterobacteria: EMB agar medium (Qingdao Hai Bo Bioisystech Co., Ltd), 37 ℃, cultivated in 24-48 hour;
Enterococcus: Sodium azide-crystal violet-aesculin agar medium (Qingdao Hai Bo Bioisystech Co., Ltd), 37 ℃, cultivated in 24-48 hour;
Bacteroid: Bd agar medium (Qingdao Hai Bo Bioisystech Co., Ltd), 37 ℃, anaerobism was cultivated in 24 hours;
C.perfringens: TSC agar medium (Qingdao Hai Bo Bioisystech Co., Ltd), 37 ℃, anaerobism was cultivated in 24 hours.
Randomly draw above-mentioned 60 routine experimenters, be divided into 3 groups (basic, normal, high dosage groups), every group 20 people, the men and women half and half.Before the test-meal given the test agent, the aseptic experimenter's ight soil of taking is put in the aseptic vessel, uses the assay balance weighing, record weight.Then, in clean bench, sterile working adds dilution (redistilled water), is diluted to 10
-2, the mixing that fully vibrates is according to 10 times of serial dilutions to 10
-8, to measure bacterium for every kind and select suitable dilution factor, inoculation is dull and stereotyped, checks above-mentioned six kinds of gut flora numbers (Bifidobacterium, lactobacillus, enterobacteria, enterococcus, bacteroid, C.perfringens).Then, basic, normal, high dosage group experimenter takes oligoisomaltose (IMO) 5g, 10g, 15g every day respectively, continuously 14d.Tried thing 3d, 7d, 10d, 14d respectively at taking, and when withdrawing back 3d, 7d, 14d, the aseptic experimenter's of taking ight soil detects and detects above-mentioned six kinds of gut flora numbers with same procedure.Observe, write down the edible front and back of experimenter subjective symptoms.
Data processing method is identical with embodiment 1.
Take 5g every day and tried thing (low dose group), take 14d, gut flora quantity testing result is as shown in table 10, and the enterobacteria of 5g/d experimental group, enterococcus, C.perfringens are taken forward and backward quantity does not have marked change (p＞0.05); Quantity increased before bacteroid 3d after taking back 10d and withdrawing took, and difference has conspicuousness (p＜0.05; Bifidobacterium is being taken back 7d, the increase of 10d quantity, and difference has conspicuousness (p＜0.05); Lactobacillus is being withdrawn back 7d quantity reduction, and difference has utmost point conspicuousness (p＜0.01).
Take every day and tried thing 10g (middle dosage group), take 14d, gut flora quantity testing result is as shown in table 11, the intestines liver bacterium of 10g/d experimental group, lactobacillus quantity no significant difference (p＞0.05); Enterococcus is being withdrawn back 14d quantity increase, and difference has conspicuousness (p＜0.05); Bacteroid wherein, is taken back 10d difference tool utmost point conspicuousness (p＜0.01) taking back 10d and withdrawing back 3d quantity increase, withdraws back 3d difference tool conspicuousness (p＜0.05); C.perfringens descends taking back 7d, 10d, 14d quantity, 10d difference tool utmost point conspicuousness (p＜0.01) wherein, 7d, 14d difference tool conspicuousness (p＜0.05); Bifidobacterium is taken back 10d difference tool utmost point conspicuousness (p＜0.01) taking back 7d, 10d and withdrawing back 7d quantity increase, takes back 7d and withdraws back 7d difference tool conspicuousness (p＜0.05).
Take every day and tried thing 15g (high dose group), take 14d, gut flora quantity testing result is as shown in table 12, and the enterococcus of 15g/d experimental group, lactobacillus quantity do not have significant variation (p＞0.05); Enterobacteria is being taken back 14d and is withdrawing back 3d quantity and reduce, and difference has conspicuousness (p＜0.05); Bacteroid 7d, 14d after taking withdraw back 7d, 14d quantity reduces, and 14d difference has utmost point conspicuousness (p＜0.01) after wherein taking back 7d and withdrawing, and taking back 14d and withdrawing back 7d difference has conspicuousness (p＜0.05); The pretty bacterium of perfringens is being taken the decline of back 7d and 10d quantity, and wherein 10d difference has utmost point conspicuousness (p＜0.01), and 7d difference has conspicuousness (p＜0.05); Bifidobacterium is being taken back 3d, the increase of 10d quantity, the equal tool utmost point of difference conspicuousness (p＜0.01).
In sum, take 14d continuously with the oligoisomaltose (IMO) of 5g/d, 10g/d, 15g/d dosage, Bifidobacterium is significantly increased.The 5g/d experimental group compares through self, and the quantity of Bifidobacterium increases by 10 times, and (P＜0.05), difference has conspicuousness; 10g/d experimental group and 15g/d experimental group compare through self, and the quantity of Bifidobacterium increases by 10 times, difference tool utmost point conspicuousness (P＜0.01).Above-mentioned experimental result shows that the Bifidobacterium quantity of each dosage group all is significantly improved, and proves that further oligoisomaltose (IMO) can obviously promote Bifidobacterium propagation in the enteron aisle.
Experimental session writes down main suit's symptom of experimenter every day, after the experimenter takes and is tried thing-oligoisomaltose (IMO) as a result, the defecation frequency rule, the ight soil proterties is normal, defecation is unobstructed, diet, sleep and the state of mind all keep well, prove that oligoisomaltose (IMO) has no adverse effects to the people, takes safety.
Table 10 take the oligoisomaltose human body intestinal canal flora dynamic observe as a result I (5g/d) (logCFU/g,
± S, n=10)
| Bacterium | Enterobacteria | Enterococcus | Bacteroid | C.perfringens | Bifidobacterium | Lactobacillus |
| Before taking | 7.11±1.38 | 5.95±1.32 | 8.05±2.28 | 1.79±0.93 | 8.00±1.81 | 8.11±1.04 |
| Take back 3d | 7.53±0.97 | 5.59±1.32 | 8.06±1.95 | 1.63±0.81 | 8.29±1.76 | 8.32±0.75 |
| 7d | 7.10±1.13 | 5.72±1.67 | 8.80±2.18 | 1.81±1.00 | 9.32±0.44* | 7.97±0.80 |
| 10d | 7.32±0.85 | 5.80±1.48 | 9.74±0.82* | 1.67±1.00 | 9.50±0.63* | 7.57±1.28 |
| 14d | 7.63±0.99 | 6.24±1.03 | 9.42±0.52 | 1.42±1.40 | 8.86±0.99 | 7.99±0.62 |
| Withdraw back 3d | 7.42±0.52 | 6.27±1.74 | 9.71±0.27* | 2.53±1.22 | 8.79±0.71 | 7.95±0.63 |
| 7d | 6.57±0.86 | 5.84±1.06 | 9.46±0.66 | 2.32±1.67 | 8.60±0.76 | 7.68±0.69 |
| 14d | 7.10±0.92 | 6.17±1.25 | 7.37±2.59 | 2.49±1.44 | 8.59±0.57 | 7.31±1.59 |
* expression: take and tried behind the thing to be tried before the thing relatively p＜0.05 with taking, * * represents: take and tried behind the thing to be tried before the thing relatively p＜0.01 with taking.
Table 11 take the oligoisomaltose human body intestinal canal flora dynamic observe as a result II (10g/d) (logCFU/g,
± S, n=10)
| Bacterium | Enterobacteria | Enterococcus | Bacteroid | C.perfringens | Bifidobacterium | Lactobacillus |
| Before taking | 6.73±1.37 | 5.21±1.11 | 8.03±2.18 | 2.00±0.99 | 7.75±1.58 | 7.24±1.36 |
| Take back 3d | 7.36±0.99 | 6.00±1.29 | 8.20±2.30 | 2.25±1.02 | 8.57±0.58 | 7.76±0.82 |
| 7d | 6.64±0.79 | 4.82±2.03 | 8.48±2.32 | 1.25±0.71* | 8.93±0.44* | 7.90±1.09 |
| 10d | 6.77±0.83 | 5.28±1.10 | 9.96±0.31** | 1.02±0.02** | 9.26±0.36** | 7.41±0.96 |
| 14d | 6.72±1.01 | 4.85±1.62 | 8.88±2.25 | 1.18±0.49* | 8.62±0.74 | 7.41±0.96 |
| Withdraw back 3d | 7.03±0.55 | 5.59±0.89 | 9.66±0.36* | 2.41±1.13 | 8.61±0.43 | 7.36±0.72 |
| 7d | 7.20±0.39 | 5.88±1.14 | 9.38±1.18 | 2.52±1.21 | 8.99±0.44* | 7.72±0.60 |
| 14d | 7.09±0.22 | 6.51±1.20* | 8.47±2.00 | 1.63±0.97 | 8.73±0.61 | 7.61±0.73 |
* expression: take and tried behind the thing to be tried before the thing relatively p＜0.05 with taking, * * represents: take and tried behind the thing to be tried before the thing relatively p＜0.01 with taking.
Table 12 take the oligoisomaltose human body intestinal canal flora dynamic observe as a result III (15g/d) (logCFU/g,
± S, n=10)
| Bacterium | Enterobacteria | Enterococcus | Bacteroid | C.perfringens | Bifidobacterium | Lactobacillus |
| Before taking | 7.72±0.67 | 5.38±0.64 | 9.91±0.24 | 2.59±1.41 | 8.69±0.46 | 7.68±1.48 |
| Take back 3d | 7.76±1.00 | 7.01±2.07 | 9.95±0.26 | 2.65±1.47 | 9.27±0.25** | 8.24±1.69 |
| 7d | 7.18±0.93 | 5.38±1.47 | 7.87±2.07** | 1.55±0.82* | 9.06±0.66 | 7.78±0.92 |
| 10d | 7.56±0.86 | 6.41±1.38 | 9.88±0.40 | 1.24±0.69** | 9.67±0.50** | 8.21±0.86 |
| 14d | 7.18±0.59* | 5.86±1.43 | 9.64±0.30* | 1.88±1.14 | 8.88±0.52 | 7.91±0.34 |
| Withdraw back 3d | 6.95±0.88* | 5.60±1.56 | 9.10±1.93 | 2.08±1.31 | 8.92±0.20 | 7.58±0.98 |
| 7d | 7.42±0.71 | 6.28±1.62 | 9.60±0.46* | 1.99±1.52 | 8.99±0.47 | 7.92±0.85 |
| 14d | 7.20±0.43 | 6.51±1.84 | 8.33±1.92** | 1.58±1.15 | 8.26±0.68 | 7.60±0.61 |
* expression: take and tried behind the thing to be tried before the thing relatively p＜0.05 with taking, * * represents: take and tried behind the thing to be tried before the thing relatively p＜0.01 with taking.
The preparation of embodiment 3, blending type milk-contained drink
Raw material standard:
Milk: protein 〉=2.95%, fat 〉=3.0%, non-fat solid 〉=8.5%.
White granulated sugar: meet country-level standard.
HFCS: F 〉=42.0%.
Oligoisomaltose (IMO): IMO 〉=50%, available from Shandong bowling treasured.
Pure water, lactic acid and citric acid all meet national standard.
Prepare blending type milk-contained drink of the present invention with reference to Fig. 1, may further comprise the steps:
1) gets material (in 1 ton) by following composition of raw materials: 300.0 kilograms in milk, 55.0 kilograms of white granulated sugars, 50.0 kilograms of HFCSs, oligoisomaltose (IMO) 20 grams, 1.6 kilograms in agar, 1.2 kilograms of sodium carboxymethylcelluloses, 1.5 kilograms of lactic acid, 2.5 kilograms of citric acids, 0.3 kilogram of strawberry essence, 567.9 kilograms of pure water;
2) agar and sodium carboxymethylcellulose and white granulated sugar are mixed, be heated in 85 ℃ the pure water, insulation dissolving 25 minutes, be cooled to 30 ℃ through cold drawing, adding fills in the material-compound tank of HFCS, oligoisomaltose (IMO) and 4 ℃ of milk again, fully stirs, and will spray with the acidity regulator (lactic acid and citric acid) that normal temperature pure water (22 ℃) fully dissolves at last to add in the material-compound tank, continue in the spray process to stir, must not be interrupted, stop;
3) with step 2) feed liquid be preheated to 35 ℃, under pressure 22Mpa (first class pressure adopts 16Mpa, and secondary pressure adopts 6Mpa), mixed liquor is carried out homogeneous;
4) will be temporarily stored in the surge tank behind the feed liquid homogeneous, add strawberry essence, stir,, obtain blending type milk-contained drink of the present invention, be cooled to 20 ℃ and carry out can 90 ℃ of heating sterilizations in 15 minutes down.
Blending type milk-contained drink with method for preparing is detected, and it is as follows to detect index:
Protein: 0.88%, fat: 0.91%, soluble solid: 13.0%, pol (in white granulated sugar): 9.0%, oligoisomaltose (IMO): 0.75%, pH:4.02.
In addition, also human body has been carried out oral experiment, experimenter's mass selection is used through 60 of all normal adults of clinical health check-up index, the men and women half and half, be divided into 3 groups of (height again, in, low dose group), every group 20 people (men and women half and half), high, in, low dose group is taken above-mentioned blending type milk-contained drink 250mL for each person every day respectively, 500mL, 750mL, with the method identical with embodiment 2 detect take before, the quantity of Bifidobacterium in the rear intestinal, after the result takes, Bifidobacterium quantity obviously improves in each experimental group people enteron aisle, and amplification can reach 10 times, shows that beverage of the present invention can obviously promote the propagation of Bifidobacterium in the enteron aisle.
The preparation of embodiment 4, blending type milk-contained drink
Raw material standard:
Milk: protein 〉=2.95%, fat 〉=3.0%, non-fat solid 〉=8.5%.
White granulated sugar: meet country-level standard.
Oligoisomaltose (IMO): IMO 〉=50%, available from Shandong bowling treasured.
Cider: AJC, pulp system pH3.7, pol 35BX.
Pure water, lactic acid and citric acid all meet national standard.
Prepare blending type milk-contained drink of the present invention with reference to Fig. 1, may further comprise the steps:
1) gets material (in 1 ton) by following composition of raw materials: 400.0 kilograms in milk, 45.0 kilograms of white granulated sugars, 15.0 kilograms of oligoisomaltoses (IMO), 15 kilograms of ciders, 3.0 kilograms of pectin, 1.0 kilograms of propylene glycol alginates, 2.0 kilograms of lactic acid, 2.5 kilograms of citric acids, 0.1 kilogram of flavoring apple essence, 516.4 kilograms of pure water;
2) pectin and propylene glycol alginate and white granulated sugar are mixed, be heated in 75 ℃ the pure water, insulation dissolving 20 minutes, be cooled to 25 ℃ through cold drawing, adding fills in the material-compound tank of cider, oligoisomaltose (IMO) and 6 ℃ of milk again, fully stirs, and will spray with the acidity regulator (lactic acid and citric acid) that normal temperature pure water (20 ℃) fully dissolves at last to add in the material-compound tank, continue in the spray process to stir, must not be interrupted, stop;
3) with step 2) feed liquid be preheated to 55 ℃, under pressure 31Mpa (first class pressure adopts 18Mpa, and secondary pressure adopts 13Mpa), mixed liquor is carried out homogeneous;
4) will be temporarily stored in the surge tank behind the feed liquid homogeneous, add flavoring apple essence, stir,, obtain blending type milk-contained drink of the present invention, be cooled to 25 ℃ and carry out can 100 ℃ of heating sterilizations in 10 minutes down.
Blending type milk-contained drink with method for preparing is detected, and it is as follows to detect index:
Protein: 1.2%, fat: 1.28%, soluble solid: 13.5%, pol (in white granulated sugar): 4.50%, oligoisomaltose (IMO): 0.56%, pH:3.90, juice content: 10%.
In addition, also human body has been carried out oral experiment, experimenter's mass selection is used through 60 of all normal adults of clinical health check-up index, the men and women half and half, be divided into 3 groups of (height again, in, low dose group), every group 20 people (men and women half and half), high, in, low dose group is taken above-mentioned blending type milk-contained drink 333mL for each person every day respectively, 666mL, 999mL, with the method identical with embodiment 2 detect take before, the quantity of Bifidobacterium in the rear intestinal, after the result takes, Bifidobacterium quantity obviously improves in each experimental group people enteron aisle, and amplification can reach 10 times, shows that beverage of the present invention can obviously promote the propagation of Bifidobacterium in the enteron aisle.
The preparation of embodiment 5, blending type milk-contained drink
Raw material standard:
Milk: protein 〉=2.95%, fat 〉=3.0%, non-fat solid 〉=8.5%.
White granulated sugar: meet country-level standard.
Oligoisomaltose (IMO): IMO 〉=50%, available from Shandong bowling treasured.
Dietary fiber: water insoluble dietary fiber 〉=60%.
Pure water, lactic acid and malic acid all meet national standard.
Prepare blending type milk-contained drink of the present invention with reference to Fig. 1, may further comprise the steps:
1) gets material (in 1 ton) by following composition of raw materials: 550.0 kilograms in milk, 90.0 kilograms of white granulated sugars, 25 kilograms of oligoisomaltoses (IMO), 0.15 kilogram of gellan gum, 2.0 kilograms of xanthans, 1.9 kilograms of lactic acid, 2.0 kilograms of malic acid, 1.0 kilograms in mango essence, 5 kilograms of dietary fibers, 322.95 kilograms of pure water;
2) gellan gum, xanthans, white granulated sugar are mixed, be heated in 65 ℃ the pure water, insulation dissolving 30 minutes, be cooled to 28 ℃ through cold drawing, adding fills in the material-compound tank of oligoisomaltose (IMO), dietary fiber and 6 ℃ of milk again, fully stirs, and will spray with the acidity regulator (lactic acid and malic acid) that normal temperature pure water (25 ℃) fully dissolves at last to add in the material-compound tank, continue in the spray process to stir, must not be interrupted, stop;
3) with step 2) feed liquid be preheated to 75 ℃, under pressure 25Mpa (first class pressure adopts 18Mpa, and secondary pressure adopts 7Mpa), mixed liquor is carried out homogeneous;
4) will be temporarily stored in the surge tank behind the feed liquid homogeneous, add flavoring apple essence, stir,, obtain blending type milk-contained drink of the present invention, be cooled to 15 ℃ and carry out can 115 ℃ of heating sterilizations in 5 minutes down.
Blending type milk-contained drink with method for preparing is detected, and it is as follows to detect index:
Protein: 1.65%, fat: 1.7%, soluble solid: 16.8%, pol (in white granulated sugar): 9.0%, oligoisomaltose (IMO): 0.9%, dietary fiber: 0.3%, pH:3.99.
In addition, also human body has been carried out oral experiment, experimenter's mass selection is used through 60 of all normal adults of clinical health check-up index, the men and women half and half, be divided into 3 groups of (height again, in, low dose group), every group 20 people (men and women half and half), high, in, low dose group is taken above-mentioned blending type milk-contained drink 200mL for each person every day respectively, 400mL, 600mL, with the method identical with embodiment 2 detect take before, the quantity of Bifidobacterium in the rear intestinal, after the result takes, Bifidobacterium quantity obviously improves in each experimental group people enteron aisle, and amplification can reach 10 times, shows that beverage of the present invention can obviously promote the propagation of Bifidobacterium in the enteron aisle.
The preparation of embodiment 6, blending type milk-contained drink
Raw material standard:
Milk: protein 〉=2.95%, fat 〉=3.0%, non-fat solid 〉=8.5%.
White granulated sugar: meet country-level standard.
Oligoisomaltose (IMO): IMO 〉=50%, available from Shandong bowling treasured.
HFCS: F 〉=42.0%.
Peach grain: flesh-content 〉=55.0%; The fruit particle size is 2-5mm; The fruit plastochondria is pH3.6; Pol 20BX.
Pure water, lactic acid and citric acid all meet national standard.
Prepare blending type milk-contained drink of the present invention with reference to Fig. 1, may further comprise the steps:
1) get material (in 1 ton) by following composition of raw materials: milk: 360.0 kilograms, white granulated sugar: 40.0 kilograms, HFCS: 60.0 kilograms, acesulfame potassium: 0.03 kilogram, oligoisomaltose (IMO): double centner, the peach grain: 120 kilograms, 3.0 kilograms of pectin, 1.0 kilograms of microcrystalline celluloses, 0.5 kilogram of single hard fatty acid ester, lactic acid: 1.7 kilograms, citric acid: 2.51 kilograms, sour milk essence: 4 kilograms, pure water: 307.26 kilograms;
2) pectin, microcrystalline cellulose, single hard fatty acid ester and white granulated sugar are mixed, be heated in 70 ℃ the pure water, insulation dissolving 25 minutes, be cooled to 18 ℃ through cold drawing, adding fills in the material-compound tank of HFCS, oligoisomaltose (IMO) and 8 ℃ of milk again, fully stirs, and will spray with the acidity regulator (lactic acid and citric acid) that normal temperature pure water (15 ℃) fully dissolves at last to add in the material-compound tank, continue in the spray process to stir, must not be interrupted, stop;
3) with step 2) feed liquid be preheated to 80 ℃, under pressure 23Mpa (first class pressure adopts 17Mpa, and secondary pressure adopts 6Mpa), mixed liquor is carried out homogeneous;
4) will be temporarily stored in the surge tank behind the feed liquid homogeneous, add essence and peach grain, stir,, obtain blending type milk-contained drink of the present invention, be cooled to 20 ℃ and carry out can 140 ℃ of heating sterilizations in 4 seconds down.
Blending type milk-contained drink with method for preparing is detected, and it is as follows to detect index:
Protein: 1.10%, fat: 1.2%, soluble solid: 13.5%, pol (in white granulated sugar): 8.5%, oligoisomaltose (IMO): 3.75%, pH:4.1, fruit grain content: 6.0%.
In addition, also human body has been carried out oral experiment, experimenter's mass selection is used through 60 of all normal adults of clinical health check-up index, the men and women half and half, be divided into 3 groups of (height again, in, low dose group), every group 20 people (men and women half and half), high, in, low dose group is taken above-mentioned blending type milk-contained drink 50mL for each person every day respectively, 100mL, 150mL, with the method identical with embodiment 2 detect take before, the quantity of Bifidobacterium in the rear intestinal, after the result takes, Bifidobacterium quantity obviously improves in each experimental group people enteron aisle, and amplification can reach 10 times, shows that beverage of the present invention can obviously promote the propagation of Bifidobacterium in the enteron aisle.
The preparation of embodiment 7, blending type milk-contained drink
Raw material standard:
Milk: protein 〉=2.95%, fat 〉=3.0%, non-fat solid 〉=8.5%.
White granulated sugar: meet country-level standard.
Oligoisomaltose (IMO): IMO 〉=50%, available from Shandong bowling treasured.
Calcium lactate: meet national standard
Strawberry juice: strawberry magma, pulp system pH 3.7, pol 35BX.
Pure water and citric acid all meet national standard.
Prepare blending type milk-contained drink of the present invention with reference to Fig. 1, may further comprise the steps:
1) get material (in 1 ton) by following composition of raw materials: milk: 450.0 kilograms, white granulated sugar: 60.0 kilograms, acesulfame potassium: 0.06 kilogram, knob is sweet: 0.004 kilogram, oligoisomaltose (IMO): 30.0 kilograms, gellan gum: 0.5 kilogram, sodium carboxymethylcellulose: 2.5 kilograms, sucrose fatty ester: 0.8 kilogram, calcium lactate: 1.0 kilograms, strawberry juice: 10 kilograms, citric acid: 4.5 kilograms, strawberry essence: 0.75 kilogram, pure water: 439.886 kilograms;
2) with gellan gum, sodium carboxymethylcellulose, sucrose fatty ester, acesulfame potassium, knob is sweet and white granulated sugar mixes, be heated in 82 ℃ the pure water, insulation dissolving 18 minutes, be cooled to 25 ℃ through cold drawing, add again and fill in the material-compound tank of oligoisomaltose (IMO), strawberry juice, calcium lactate and 6.5 ℃ of milk, fully stir, to add in the material-compound tank with acidity regulator (citric acid) spray that normal temperature pure water (20 ℃) fully dissolves at last, continue in the spray process to stir, must not be interrupted, stop;
3) with step 2) feed liquid be preheated to 75 ℃, under pressure 40Mpa (first class pressure adopts 23Mpa, and secondary pressure adopts 17Mpa), mixed liquor is carried out homogeneous;
4) will be temporarily stored in the surge tank behind the feed liquid homogeneous, add essence, stir,, obtain blending type milk-contained drink of the present invention, be cooled to 27 ℃ and carry out can 121 ℃ of heating sterilizations in 5 seconds down.
Blending type milk-contained drink with method for preparing is detected, and it is as follows to detect index:
Protein: 1.44%, fat: 1.52%, soluble solid: 12.9%, pol (in white granulated sugar): 6.0%, oligoisomaltose: 1.1%, pH:4.0, calcium lactate: 75mg, juice content 15%.
In addition, also human body has been carried out oral experiment, experimenter's mass selection is used through 60 of all normal adults of clinical health check-up index, the men and women half and half, be divided into 3 groups of (height again, in, low dose group), every group 20 people (men and women half and half), high, in, low dose group is taken above-mentioned blending type milk-contained drink 167mL for each person every day respectively, 334mL, 501mL, with the method identical with embodiment 2 detect take before, the quantity of Bifidobacterium in the rear intestinal, after the result takes, Bifidobacterium quantity obviously improves in each experimental group people enteron aisle, and amplification can reach 10 times, shows that beverage of the present invention can obviously promote the propagation of Bifidobacterium in the enteron aisle.
The preparation of embodiment 8, blending type milk-contained drink
Raw material standard:
Milk: protein 〉=2.95%, fat 〉=3.0%, non-fat solid 〉=8.5%.
White granulated sugar: meet country-level standard.
Oligoisomaltose (IMO): IMO 〉=50%, available from Shandong bowling treasured.
HFCS: F 〉=42.0%.
Pure water, lactic acid, malic acid: meet national standard.
Ironic citrate: meet national standard.
Prepare blending type milk-contained drink of the present invention with reference to Fig. 1, may further comprise the steps:
1) gets material (in 1 ton) by following composition of raw materials: 500 kilograms in milk, 50.0 kilograms of white granulated sugars, 50.0 kilograms of HFCSs, 50.0 kilograms of oligoisomaltoses (IMO), 1.0 kilograms of konjac glucomannans, 2.0 kilograms of sodium carboxymethylcelluloses, 1.5 kilograms of lactic acid, malic acid: 2.5 kilograms, 0.3 kilogram of orange flavor, 1.0 kilograms of ironic citrates, 341.7 kilograms of pure water;
2) konjac glucomannan, sodium carboxymethylcellulose, ironic citrate and white granulated sugar are mixed, be heated in 85 ℃ the pure water, insulation dissolving 25 minutes, be cooled to 30 ℃ through cold drawing, adding fills in the material-compound tank of HFCS, oligoisomaltose (IMO) and 4 ℃ of milk again, fully stirs, and will spray with the acidity regulator (lactic acid and malic acid) that normal temperature pure water (22 ℃) fully dissolves at last to add in the material-compound tank, continue in the spray process to stir, must not be interrupted, stop;
3) with step 2) feed liquid be preheated to 65 ℃, under pressure 22Mpa (first class pressure adopts 16Mpa, and secondary pressure adopts 6Mpa), mixed liquor is carried out homogeneous;
4) will be temporarily stored in the surge tank behind the feed liquid homogeneous, add essence, stir,, obtain blending type milk-contained drink of the present invention, be cooled to 15 ℃ and carry out can 115 ℃ of heating sterilizations in 4 seconds down.
Blending type milk-contained drink with method for preparing is detected, and it is as follows to detect index:
Protein: 1.4%, fat: 1.6%, soluble solid: 14.0%, pol (in white granulated sugar): 8.5%, oligoisomaltose (IMO): 1.8%, pH:4.02, ironic citrate (mg/100g): 100.
In addition, also human body has been carried out oral experiment, experimenter's mass selection is used through 60 of all normal adults of clinical health check-up index, the men and women half and half, be divided into 3 groups of (height again, in, low dose group), every group 20 people (men and women half and half), high, in, low dose group is taken above-mentioned blending type milk-contained drink 100mL for each person every day respectively, 200mL, 300mL, with the method identical with embodiment 2 detect take before, the quantity of Bifidobacterium in the rear intestinal, after the result takes, Bifidobacterium quantity obviously improves in each experimental group people enteron aisle, and amplification can reach 10 times, shows that beverage of the present invention can obviously promote the propagation of Bifidobacterium in the enteron aisle.
The preparation of embodiment 9, blending type milk-contained drink
Raw material standard:
Milk: protein 〉=2.95%, fat 〉=3.0%, non-fat solid 〉=8.5%.
White granulated sugar: meet country-level standard.
Oligoisomaltose (IMO): IMO 〉=90%, available from Shandong bowling treasured.
Orange juice: sweet orange inspissated juice, pulp system pH 3.6, pol 42BX.
Pure water, lactic acid, citric acid: meet national standard.
Prepare blending type milk-contained drink of the present invention with reference to Fig. 1, may further comprise the steps:
1) gets material (in 1 ton) by following composition of raw materials: milk: 600.0 kilograms, white granulated sugar: 70.0 kilograms, 74.0 kilograms of oligoisomaltoses (IMO), orange juice: 30 kilograms, pectin: 3.5 kilograms, locust bean gum: 1.0 kilograms, lactic acid: 2.0 kilograms, citric acid: 2.5 kilograms, orange flavor: 0.1 kilogram, pure water: 216.9 kilograms;
2) pectin, locust bean gum and white granulated sugar are mixed, be heated in 70 ℃ the pure water, insulation dissolving 20 minutes, be cooled to 25 ℃ through cold drawing, adding fills in the material-compound tank of orange juice, oligoisomaltose (IMO) and 6 ℃ of milk again, fully stirs, and will spray with the acidity regulator (lactic acid and citric acid) that normal temperature pure water (20 ℃) fully dissolves at last to add in the material-compound tank, continue in the spray process to stir, must not be interrupted, stop;
3) with step 2) feed liquid be preheated to 55 ℃, under pressure 18Mpa (first class pressure adopts 13Mpa, and secondary pressure adopts 5Mpa), mixed liquor is carried out homogeneous;
4) will be temporarily stored in the surge tank behind the feed liquid homogeneous, add essence, stir,, obtain blending type milk-contained drink of the present invention, be cooled to 25 ℃ and carry out can 110 ℃ of heating sterilizations in 1 minute down.
Blending type milk-contained drink with method for preparing is detected, and it is as follows to detect index:
Protein: 1.7%, fat: 1.9%, soluble solid: 15.5%, pol (in white granulated sugar): 7.0%, oligoisomaltose (IMO): 5%, pH:4.0, juice content: 20%.
In addition, also human body has been carried out oral experiment, experimenter's mass selection is used through 60 of all normal adults of clinical health check-up index, the men and women half and half, be divided into 3 groups of (height again, in, low dose group), every group 20 people (men and women half and half), high, in, low dose group is taken above-mentioned blending type milk-contained drink 37.6mL for each person every day respectively, 75.2mL, 112.8mL, with the method identical with embodiment 2 detect take before, the quantity of Bifidobacterium in the rear intestinal, after the result takes, Bifidobacterium quantity obviously improves in each experimental group people enteron aisle, and amplification can reach 10 times, shows that beverage of the present invention can obviously promote the propagation of Bifidobacterium in the enteron aisle.
The preparation of embodiment 10, blending type milk-contained drink
Raw material standard:
Milk: protein 〉=2.95%, fat 〉=3.0%, non-fat solid 〉=8.5%.
White granulated sugar: meet country-level standard.
Oligoisomaltose (IMO): IMO 〉=50%, available from Shandong bowling treasured.
The strawberry grain: flesh-content 〉=65.0%, fruit grain diameter: 3-11mm, pulp is fresh, and the normal fragrance of strawberry is arranged, pH:4.5, pol: 18BX.
Pure water, lactic acid, malic acid: meet national standard.
Prepare blending type milk-contained drink of the present invention with reference to Fig. 1, may further comprise the steps: milk: 350.0 kilograms, white granulated sugar: 85.0 kilograms, oligoisomaltose (IMO): 10.0 kilograms, propylene glycol alginate: 1.5 kilograms, xanthans: 2.0 kilograms, the strawberry grain: 60.0 kilograms, lactic acid: 2.0 kilograms, malic acid: 1.5 kilograms, strawberry essence: 1.0 kilograms, pure water: 495.0 kilograms;
2) propylene glycol alginate, xanthans and white granulated sugar are mixed, be heated in 65 ℃ the pure water, insulation dissolving 30 minutes, be cooled to 28 ℃ through cold drawing, adding fills in the material-compound tank of oligoisomaltose (IMO) and 6 ℃ of milk again, fully stirs, and will spray with the acidity regulator (lactic acid and malic acid) that normal temperature pure water (25 ℃) fully dissolves at last to add in the material-compound tank, continue in the spray process to stir, must not be interrupted, stop;
3) with step 2) feed liquid be preheated to 55 ℃, under pressure 25Mpa (first class pressure adopts 18Mpa, and secondary pressure adopts 7Mpa), mixed liquor is carried out homogeneous;
4) will be temporarily stored in the surge tank behind the feed liquid homogeneous, add strawberry grain, essence, stir,, obtain blending type milk-contained drink of the present invention, be cooled to 5 ℃ and carry out can 95 ℃ of heating sterilizations in 10 minutes down.
Blending type milk-contained drink with method for preparing is detected, and it is as follows to detect index:
Protein: 1.03%, fat: 1.1%, soluble solid: 15.8%, pol (in white granulated sugar): 8.5%, oligoisomaltose (IMO): 0.37%, pH:4.15, pulp: 3.60%.
In addition, also human body has been carried out oral experiment, experimenter's mass selection is used through 60 of all normal adults of clinical health check-up index, the men and women half and half, be divided into 3 groups of (height again, in, low dose group), every group 20 people (men and women half and half), high, in, low dose group is taken above-mentioned blending type milk-contained drink 500mL for each person every day respectively, 1000mL, 1500mL, with the method identical with embodiment 2 detect take before, the quantity of Bifidobacterium in the rear intestinal, after the result takes, Bifidobacterium quantity obviously improves in each experimental group people enteron aisle, and amplification can reach 10 times, shows that beverage of the present invention can obviously promote the propagation of Bifidobacterium in the enteron aisle.
The check of embodiment 11, product mouthfeel
Blending type milk-contained drink with embodiment 3-10 preparation is a laboratory sample, carries out taste tests, and method is as follows:
Test number: 300 people;
The trial test mode: adopt the mode of blank marking to carry out, the full marks of local flavor, color and luster, mouthfeel, chewiness four indices are 10 minutes; The full marks of hardness, sour-sweet ratio and three indexs of nutritive value are 20 minutes; Mark is high more, and the expression effect is good more, carries out statistical analysis to tasting the result, and the result is as shown in table 13, show of the present invention contain oligoisomaltose (IMO) though the blending type milk-contained drink be in local flavor, mouthfeel or tangible improvement all arranged on nutritional labeling.
Table 13 product of the present invention is tasted the result data table
| Index and mark | Embodiment 3 | Embodiment 4 | Embodiment 5 | Embodiment 6 | Embodiment 7 | Embodiment 8 | Embodiment 9 | Embodiment 10 | |
| Local flavor (10) | 8.0 | 8.2 | 7.8 | 7.5 | 9.0 | 8.5 | 8.1 | 7.7 | |
| Color and luster (10) | 9.0 | 8.0 | 8.5 | 8.8 | 8.5 | 8.9 | 8.2 | 8.3 | |
| Mouthfeel (20) | 17.0 | 18.0 | 17.5 | 19.0 | 17.0 | 19 | 18.5 | 18.0 | |
| Chewiness (20) | 18.0 | 17.5 | 19.0 | 17.8 | 18.0 | 16.5 | 19.5 | 18.5 | |
| Sugar-acid ratio (20) | 17.0 | 18.0 | 16.5 | 17.5 | 18.5 | 18.7 | 17.7 | 18.3 | |
| Nutritive value (20) | 19.0 | 18.5 | 17.50 | 17.0 | 18.0 | 19.2 | 18.3 | 19.5 | |
| Totally comment (number) | Like | 230.0 | 240.0 | 251.0 | 225.0 | 232.0 | 235 | 245 | 217 |
| Well | 50.0 | 43.0 | 30.0 | 55.0 | 38.0 | 40 | 38 | 60 | |
| Generally | 20.0 | 17.0 | 19.0 | 20.0 | 30.0 | 25 | 17 | 23 | |
Claims (10)
1, a kind of blending type milk-contained drink, its prescription comprises following component:
Milk 30-60g,
Oligoisomaltose 0.01-10.0g,
Sugar 4.0-12.0g,
Stabilizing agent 0.1-1.0g,
Acidity regulator 0.2-0.7g,
Flavoring essence 0.01-0.4g,
Add water to 100g.
2, blending type milk-contained drink according to claim 1 is characterized in that: described stabilizing agent is emulsifying agent or thickener, or is combined by emulsifying agent and thickener, and wherein, the ratio of weight and number of emulsifying agent and thickener is 1: 1-60; Described emulsifying agent is one or more the combination in sucrose fatty ester, fatty acid glyceride and the polyglycerol ester; Described thickener is one or more the combination in sodium carboxymethylcellulose, carragheen, gelatin, microcrystalline cellulose, gellan gum, xanthans, propylene glycol alginate, konjac glucomannan, agar, locust bean gum and the pectin.
3, blending type milk-contained drink according to claim 1 is characterized in that: described acidity regulator is one or more the combination in lactic acid, citric acid, phosphoric acid, tartaric acid and the malic acid; Described flavoring essence is a sour milk essence, milk flavour, orange essence, grape essence, peach essence, cocoanut flavour, strawberry essence, lychee flavor, flavoring apple essence, close melon essence, mango essence, passionflower essence, grapefruit essence, lemon extract, blueberry essence, pawpaw essence, flavoring banana essence, flavoring pineapple essence, tomato flavour, carrot essence, asparagus essence, spinach essence, apricot essence, pear essence, kiwi flavor, vanilla, flavoring rose essence, chocolate essence, walnut essence, the combination of one or more in wheat essence and the cherry essence; Described sugar is any one or a few the mixing in white granulated sugar, syrup and the sweetener.
4, according to each described blending type milk-contained drink of claim 1-3, it is characterized in that: also include 0.5-20g Juice or fruit-vegetable granules in the described prescription, and/or the 0.01-0.5g trace element, and/or the 0.3-0.8g dietary fiber; Described Juice is selected from one or more the combination in cider, strawberry juice, blueberry juice, Chinese flowering quince juice, bananas juice, orange juice, pineapple juice, mango juice, tomato juice, carrot juice, asparagus juice, spinach juice, peach juice, apricot juice, pear juice, grape juice, asparagus juice, kiwi-fruit juice, Lychee juice, lemon juice and the Coconut Juice; Described fruit-vegetable granules is selected from the really combination of one or more in grain, celery particle, the operatic circle grain, cucumber particle, radish kind particle and the aloe gel particle of tomato particle, peach particle, coconut palm fruit granule, orange fruit grain, mango fruit grain, grape fruit, pawpaw fruit grain, pineapple fruit grain, strawberry; Described trace element is selected from one or more the combination in vitamin A, vitamin B complex, vitamin D, vitamin C, vitamin E, calcium lactate, taurine, folic acid, nicotinic acid, choline, ferrous sulfate, ironic citrate, zinc sulfate, zinc gluconate, sodium selenite, magnesium sulfate, magnesium gluconate and the l-cn; Described fruit-vegetable granules is granular garden stuff grain or the slurry class fruit and vegetable of diameter 1-20mm, or length is that 1-20mm, width are the fibrous fruit and vegetable of 1-10mm.
5, a kind of method for preparing the described blending type milk-contained drink of claim 1 may further comprise the steps:
1) get material by following composition of raw materials: milk 30-60g, oligoisomaltose 0.01-10.0g, sugared 4.0-12.0g, stabilizing agent 0.1-1.0g, acidity regulator 0.2-0.7g, flavoring essence 0.01-0.4g adds water to 100g;
2) sugar and stabilizing agent water dissolving back are fully mixed with milk, under stirring condition, add acidity regulator again, mixing;
3) with step 2) mixed liquor be preheated to 25-80 ℃, under pressure 13-40Mpa, it is carried out homogeneous again;
4) add flavoring essence, stir, sterilization obtains the blending type milk-contained drink.
6, preparation method according to claim 5 is characterized in that: the stabilizing agent in the described step 1) is emulsifying agent or thickener, or is combined by emulsifying agent and thickener, and wherein, the ratio of weight and number of emulsifying agent and thickener is 1: 1-60; Described emulsifying agent is selected from one or more the combination in sucrose fatty ester, fatty acid glyceride and the polyglycerol ester; Thickener is selected from one or more the combination in sodium carboxymethylcellulose, carragheen, gelatin, microcrystalline cellulose, gellan gum, xanthans, propylene glycol alginate, konjac glucomannan, agar, locust bean gum and the pectin.
7, preparation method according to claim 5 is characterized in that: the acidity regulator in the described step 1) is selected from one or more the combination in lactic acid, citric acid, phosphoric acid, tartaric acid and the malic acid; Described flavoring essence is selected from sour milk essence, milk flavour, orange essence, grape essence, peach essence, cocoanut flavour, strawberry essence, lychee flavor, flavoring apple essence, close melon essence, mango essence, passionflower essence, grapefruit essence, lemon extract, blueberry essence, pawpaw essence, flavoring banana essence, flavoring pineapple essence, tomato flavour, carrot essence, asparagus essence, spinach essence, apricot essence, pear essence, kiwi flavor, vanilla, flavoring rose essence, chocolate essence, walnut essence, the combination of one or more in wheat essence and the cherry essence; Described sugar is selected from any one or a few the mixing in white granulated sugar, syrup and the sweetener.
8, preparation method according to claim 5 is characterized in that: add 0.5-20g Juice and/or fruit-vegetable granules in the described preparation process again, and/or the 0.01-0.5g trace element, and/or the 0.3-0.8g dietary fiber; Described Juice, trace element and/or dietary fiber are in step 2) in add, after the adding again with the acidity regulator mixing; Fruit-vegetable granules adds in step 4), sterilization again after the adding; Described Juice is selected from one or more the combination in cider, strawberry juice, blueberry juice, Chinese flowering quince juice, bananas juice, orange juice, pineapple juice, mango juice, tomato juice, carrot juice, asparagus juice, spinach juice, peach juice, apricot juice, pear juice, grape juice, asparagus juice, kiwi-fruit juice, Lychee juice, lemon juice and the Coconut Juice; Described trace element is selected from one or more the combination in vitamin A, vitamin B complex, vitamin D, vitamin C, vitamin E, calcium lactate, taurine, folic acid, nicotinic acid, choline, ferrous sulfate, ironic citrate, zinc sulfate, zinc gluconate, sodium selenite, magnesium sulfate, magnesium gluconate and the l-cn; Described fruit-vegetable granules is selected from the really combination of one or more in grain, celery particle, the operatic circle grain, cucumber particle, radish kind particle and the aloe gel particle of tomato particle, peach particle, coconut palm fruit granule, orange fruit grain, mango fruit grain, grape fruit, pawpaw fruit grain, pineapple fruit grain, strawberry.
9, preparation method according to claim 8 is characterized in that: described fruit-vegetable granules is granular garden stuff grain or the slurry class fruit and vegetable of diameter 1-20mm, or length is that 1-20mm, width are the fibrous fruit and vegetable of 1-10mm.
10, according to each described preparation method of claim 5-9, it is characterized in that: the sterilising conditions in the described step 4) was: 90-140 ℃ of following sterilization 4 seconds to 15 minutes.
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| CNA2007100001767A CN1994100A (en) | 2007-01-09 | 2007-01-09 | A formulated milk-contained drink and method for preparing same |
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| CNA2007100001767A CN1994100A (en) | 2007-01-09 | 2007-01-09 | A formulated milk-contained drink and method for preparing same |
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