CN1969889A - Enteric-coated multilayer encapsulated probiotic microcapsule and preparation method thereof - Google Patents
Enteric-coated multilayer encapsulated probiotic microcapsule and preparation method thereof Download PDFInfo
- Publication number
- CN1969889A CN1969889A CNA2006100705633A CN200610070563A CN1969889A CN 1969889 A CN1969889 A CN 1969889A CN A2006100705633 A CNA2006100705633 A CN A2006100705633A CN 200610070563 A CN200610070563 A CN 200610070563A CN 1969889 A CN1969889 A CN 1969889A
- Authority
- CN
- China
- Prior art keywords
- microcapsule
- enteric
- sodium alginate
- alginate
- calcium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicinal Preparation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses an enteric-solubility multilayer encysted bacterium microcapsule and making method in the biological agent technical domain, which is characterized by the following: adopting sodium alginate, calcium chloride and chitose as clad material of microcapsule; making lactic acid bacteria or bifidobacteria as core-clad material; proceeding ionic exchange for sodium alginate and calcium chloride; forming second clad on the surface of calcium alginate through different isoelectric points of calcium alginate and chitose; freezing at low temperature to obtain the product.
Description
One, technical field
The present invention relates to a kind of microorganism formulation, be specifically related to a kind of enteric-coated multilayer encapsulated probiotic microcapsule and preparation method thereof.
Two, background technology
Microorganism formulation is as nontoxic, the no Drug resistance of a kind of natural biologically active agents, noresidue, and have enhance immunity, promote growth, improve the effect of efficiency of feed utilization, be widely used in human health care's food, and can be used as feed additive and be applied in the breed of livestock-raising and aquatic animal.Referring to disclosed Chinese patent application 200510045358.7 on July 12nd, 2006, " multifunctional probiotic preparation and preparation method thereof ".
Probiotics preparation mainly is an active bacteria formulation, is obligate anaerobe mostly, and lactobacillus wherein and bacillus bifidus are a class anaerobe, is difficult to survival in air, under drying or the room temperature condition, and the shelf-life of its product is shortened.After lactobacillus and bacillus bifidus are eaten by the human or animal simultaneously, can not tolerate stomach sour environment (pH value 1~2), cause probiotic bacteria death can not reach required benefit and come into force really at human or animal's gastric.
CN1613455 discloses a kind of targeting probiotic microcapsule, this microcapsule is made up of probiotic bacteria and/or protective agent and three-layer protection layer, and wherein the ground floor protective layer forms primary microcapsules for the protein after adopting T-5398 crosslinked with the probiotic bacteria embedding; Second layer protective layer be with primary microcapsules with after the suitable dilution agent mixes, coat at 30-40 ℃ hydrogenated oil and fat with fusing point and to form secondary microcapsule; The three-layer protection layer is a release controlling coating material.The weak point of this method is: bag is by complex process, and the operating time is long; In operating process, probiotic bacteria is long-time contact with air or operating process in temperature drift or change too fastly, cause the easy inactivation of thalline; And the part capsulating material has certain influence to the thalline activity, is difficult for long preservation.
Three, summary of the invention
At the deficiencies in the prior art, the invention provides a kind of enteric-coated multilayer encapsulated probiotic microcapsule and preparation method thereof.
Enteric-coated multilayer encapsulated probiotic microcapsule of the present invention, with lactobacillus or bacillus bifidus is core-clad material, sodium alginate, calcium chloride and chitosan are the microcapsule capsulating material, alginate is by β-1 in the capsulating material, 4-D type mannuronic acid (M) and α-1,4-L type guluronic acid (G) copolymerization forms.At Ca2
+Forming gel in the solution utilizes sodium alginate and calcium chloride generation ion exchange to form calcium alginate bag quilt, it forms one deck semipermeable membrane on every side at alginate (electronegative), utilize the different chitosans that make with the chitosan isoelectric point, IP of calcium alginate to form secondary bag quilt on the calcium alginate surface, frozen drying is made.
Above-mentioned probiotic microcapsule particle diameter is at 10~100 μ m.
Calcium alginate has good acid resistance in the capsulating material of the present invention, chitosan is a kind of positively charged polymer, can interosculate so have good intestinal adsorptivity with electronegative intestinal epithelial cell, the gained coated multilayer encapsulated probiotic microcapsule can long preservation also can be located the effect that discharges at intestinal.The health food or the animal feed additive that can be used for the people.
The preparation method of enteric-coated multilayer encapsulated probiotic microcapsule of the present invention, step is as follows:
1. with lactobacillus or the centrifugal collection wet thallus of bifidus bacillus fermented liquid, wet thallus washed out with the phosphate buffer of normal saline or pH value 6.8 be bacteria suspension.
2. the bacteria suspension 1: 5 by volume~10 of gained wet thallus is joined mixing in the sodium alginate soln of 1~2%wt, sodium alginate thalline suspension.
3. sodium alginate thalline suspension is made little drop, join in the calcium chloride solution of 2~4%wt and stir, little drop and calcium chloride solution volume ratio 1: 1~2 obtain wrapping the microcapsule granule by calcium alginate.
4. centrifugal collection microcapsule granule joins in the chitosan solution of 1~2%wt and carries out secondary bag quilt.Microcapsule granule and chitosan solution volume ratio 1: 1~1.5.
5. centrifugal collection microcapsule granule, lyophilization gets enteric-coated multilayer encapsulated probiotic microcapsule.
Method of the present invention is carried out at normal temperatures, simple to operate, selected capsulating material all is the harmless natural organic substance of edible safety, and outermost layer capsulating material oligochitosan has the immunity of raising, suppresses the growth of cancerous protuberance cell, promote liver spleen antibody to form, promote the absorption of calcium and mineral, promote human body beneficial's flora propagation such as bacillus bifidus, lactobacillus, blood fat reducing, blood pressure lowering, blood sugar lowering, cholesterol regulating, fat-reducing, functions such as prevention adult disease.Method for coating of the present invention need not be regulated acid-base value, also needn't use fixative (as harmful substances such as formaldehyde).
Method operating time of the present invention is short, makes thalline open-assembly time in air short, and capsulating material is harmless and edible safety to thalline; Microcapsule behind the bag quilt carries out frozen drying, can prolong the holding time of probiotic bacteria, and the product after the lyophilization can be preserved under 2~8 ℃ condition 5~10 years, can preserve more than 2 years under the room temperature condition.
The present invention is wrapped in the microcapsule lactobacillus or bacillus bifidus by preparation enteric solubility microcapsule, and secluding air can tolerate the stomach sour environment simultaneously.Because the isoelectric point, IP of microencapsulated material is opposite with the isoelectric point, IP of intestinal epithelial cell, can make microcapsule be adsorbed in the enteric epithelium surface, and microcapsule dissolves in intestinal juice, reach the effect that the location discharges, make lactobacillus or bacillus bifidus can arrive the position growth and breeding of suitable field planting, play benefit and come into force really.
Four, the specific embodiment
The present invention will be further described below in conjunction with embodiment, but be not limited thereto.
Embodiment 1:
(1) sodium alginate is made into 2% solution 100ml, prepares 4% calcium chloride solution 100ml, preparation 1%wt chitosan solution 100ml.
(2) with the centrifugal collection wet thallus of 500ml streptococcus acidi lactici fermented solution, wet thallus is suspended in the 10ml normal saline.
(3) suspension with wet thallus adds mixing in the above-mentioned sodium alginate soln, gets sodium alginate thalline suspension.
(4) sodium alginate thalline suspension is made little drop, add in the above-mentioned calcium chloride solution and stir, obtain the sodium alginate microcapsule granule.
(5) centrifugal collection microcapsule granule joins and carries out secondary bag quilt in the above-mentioned 1%wt chitosan solution.
(6) microcapsule granule of centrifugal collection derma, lyophilization.
(7) dried microcapsule granule diameter is at 10~100 μ m.
Microcapsule to present embodiment is checked as follows:
1, gets two parts of the freeze dried microcapsule granules of 0.1g, carry out the test of gastric solubility test and enteric solubility respectively and count plate.
2, preparation simulated gastric fluid: hydrochloric acid 16.4mL, pepsin 10g adds and is settled to 1000mL, pH=1.2 after water stirs evenly.Simulated intestinal fluid: potassium dihydrogen phosphate 6.8g, add water 500mL dissolving, the sodium hydroxide solution with 0.4% is regulated pH value to 6.8; Other gets pancreatin 10g and adds water and make its dissolving in right amount, after two liquid are mixed, adds water and is settled to 1000mL.
3, get microcapsule 0.1g and place the triangular flask that fills 50mL, pH1.2 simulated gastric fluid, placing (37 ± 1) ℃ constant temperature, rotating speed again is on the shaking table of 150r/min, the situation of change of microcapsule light transmittance in simulated gastric fluid.Microcapsule contacts 3h with gastric juice, and light transmittance remains unchanged substantially in the treatment fluid, therefore can prove that probiotic microcapsule can tolerate the destruction of gastric juice.
4, get microcapsule 0.1g and place the triangular flask that fills 50mL, pH6.8 simulated intestinal fluid, placing (37 ± 1) ℃ constant temperature, rotating speed again is on the shaking table of 150r/min, microcapsule disintegrate fully behind 45min judges that thus microcapsule can discharge probiotic bacteria in the 45min in simulation intestinal environment.
5, with after the disintegrate bacteria suspension carry out flat board and pour into the methods,anaerobic count plate, the microcapsule after the lyophilizing on average contains viable bacteria 1~2 * 10
10CFU/mL.
Embodiment 2:
With the centrifugal collection wet thallus of 500ml bifidus bacillus fermented liquid, be diluted to 10ml with the phosphate buffer of pH value 6.8.Gained wet thallus suspension is joined mixing in 2% sodium alginate soln of 100ml, sodium alginate thalline suspension.Sodium alginate thalline suspension is made little drop, join in the calcium chloride solution of 100ml 3% and stir, obtain wrapping microcapsule granule by calcium alginate.
The above-mentioned microcapsule granule of centrifugal collection joins in 2% the chitosan solution of 100ml again and carries out secondary bag quilt.
The above-mentioned microcapsule granule of centrifugal collection, lyophilization, dried microcapsule granule diameter is at 10~100 μ m.Get enteric-coated multilayer encapsulated probiotic microcapsule.
Claims (3)
1, enteric-coated multilayer encapsulated probiotic microcapsule, it is characterized in that with lactobacillus or bacillus bifidus be core-clad material, sodium alginate, calcium chloride and chitosan are the microcapsule capsulating material, utilize sodium alginate and calcium chloride generation ion exchange to form calcium alginate bag quilt, utilize the different chitosans that make with the chitosan isoelectric point, IP of calcium alginate to form secondary bag quilt on the calcium alginate surface again, frozen drying is made.
2, enteric-coated multilayer encapsulated probiotic microcapsule as claimed in claim 1 is characterized in that described microcapsule granule diameter is at 10~100 μ m.
3, a kind of preparation method of enteric-coated multilayer encapsulated probiotic microcapsule, step is as follows:
(1) the centrifugal collection wet thallus of lactobacillus or bifidus bacillus fermented liquid washes out with the phosphate buffer of normal saline or pH value 6.8 and to be bacteria suspension;
(2) bacteria suspension of gained wet thallus 1: 5 by volume~10 joins mixing in the sodium alginate soln of 1~2%wt, sodium alginate thalline suspension;
(3) sodium alginate thalline suspension is made little drop, join in the calcium chloride solution of 2~4%wt and stir, little drop and calcium chloride solution volume ratio 1: 1~2 obtain wrapping the microcapsule granule by calcium alginate;
(4) centrifugal collection microcapsule granule joins and carries out secondary bag quilt in above-mentioned 1~2%wt chitosan solution; Microcapsule granule and chitosan solution volume ratio 1: 1~1.5;
(5) centrifugal collection microcapsule granule, lyophilization gets enteric-coated multilayer encapsulated probiotic microcapsule.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB2006100705633A CN100537759C (en) | 2006-12-04 | 2006-12-04 | Enteric-coated multilayer encapsulated probiotic microcapsule and preparation method thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB2006100705633A CN100537759C (en) | 2006-12-04 | 2006-12-04 | Enteric-coated multilayer encapsulated probiotic microcapsule and preparation method thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1969889A true CN1969889A (en) | 2007-05-30 |
CN100537759C CN100537759C (en) | 2009-09-09 |
Family
ID=38111037
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB2006100705633A Expired - Fee Related CN100537759C (en) | 2006-12-04 | 2006-12-04 | Enteric-coated multilayer encapsulated probiotic microcapsule and preparation method thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN100537759C (en) |
Cited By (65)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102198115A (en) * | 2011-05-19 | 2011-09-28 | 甘肃易源生物制药有限公司 | Enteric-coated micro-capsule prepared by mixing traditional Chinese medicine extract and probitics |
CN102334590A (en) * | 2011-08-10 | 2012-02-01 | 广东工业大学 | Method for recycling waste yogurt |
CN101691568B (en) * | 2009-10-13 | 2012-02-08 | 哈尔滨美华生物技术股份有限公司 | Method of producing edible immobilized active probiotics |
CN101249109B (en) * | 2008-03-14 | 2012-06-27 | 华侨大学 | Lactobacillus reuteri hollow micro-capsule and its preparing method |
CN102613458A (en) * | 2012-04-16 | 2012-08-01 | 广东仙乐制药有限公司 | Probiotics soft capsule and preparation method thereof |
CN102640933A (en) * | 2012-04-17 | 2012-08-22 | 中国科学院烟台海岸带研究所 | Phycocyanin microcapsule and preparation method of phycocyanin microcapsule |
CN102919542A (en) * | 2012-11-13 | 2013-02-13 | 西华大学 | Multistage-controlled-release probiotics microecological preparation composite feed for Silurus meridionalis and preparation method thereof |
CN102960600A (en) * | 2012-11-19 | 2013-03-13 | 陕西科技大学 | Method for preparing two-layer embedded bifidobacterium microcapsules |
CN103005463A (en) * | 2012-12-07 | 2013-04-03 | 北京百奥未来生物科技有限公司 | Low molecular collagen oral enteric microcapsule, preparation method thereof, and health drink |
CN103222539A (en) * | 2013-04-09 | 2013-07-31 | 魏永刚 | Preparation method of microbial pre-fermentation coating multilayer microcapsule |
CN103357062A (en) * | 2012-03-26 | 2013-10-23 | 约泰实业股份有限公司 | Fibrous hydrogel and preparation method thereof |
CN103355656A (en) * | 2012-04-01 | 2013-10-23 | 中国科学院大连化学物理研究所 | Probiotics microcapsule product and preparation and application thereof |
WO2013166681A1 (en) * | 2012-05-10 | 2013-11-14 | 聚和国际股份有限公司 | Composition for producing microcapsules and medicine made thereof |
CN103431379A (en) * | 2013-07-31 | 2013-12-11 | 吉林农业大学 | Method for producing suspended lactobacillus wet capsule with low energy consumption |
CN103458709A (en) * | 2011-01-21 | 2013-12-18 | 凡赛尔医药股份有限公司 | Microencapsulated probiotic substance and process of manufacture |
CN103704718A (en) * | 2013-12-23 | 2014-04-09 | 安徽大学 | Preparation method of bacillus subtilis microcapsule with high viable count in shelf life |
CN104000025A (en) * | 2014-06-11 | 2014-08-27 | 北京昕地美饲料科技有限公司 | Method for making stabilized lactic acid bacteria product |
CN104431370A (en) * | 2014-12-15 | 2015-03-25 | 南京优帆生物科技有限公司 | Efficient probiotics microcapsule as well as preparation method and application thereof |
CN104644612A (en) * | 2015-01-26 | 2015-05-27 | 天津科技大学 | Sodium polyacrylate-grafted and sodium alginate-embedded probiotic microcapsule and preparation method thereof |
CN104887647A (en) * | 2014-03-08 | 2015-09-09 | 复旦大学 | Probiotics double-layered microcapsule and manufacturing method thereof |
CN104957254A (en) * | 2015-06-08 | 2015-10-07 | 内蒙古蒙牛乳业(集团)股份有限公司 | Probiotics gel particle, preparation method thereof and dairy containing probiotics gel particle |
CN104970368A (en) * | 2015-06-30 | 2015-10-14 | 安徽大学 | Preparation method of gastric acid-resistant bacillus licheniformis microcapsules with high survival rate |
CN105963344A (en) * | 2016-07-13 | 2016-09-28 | 中国科学院海洋研究所 | Fermented traditional Chinese medicine preparation for improving immunity of aquaculture animals and preparation method thereof |
CN106343578A (en) * | 2016-08-29 | 2017-01-25 | 河北然生物科技有限公司 | Enteric double-layer seamless solid probiotic microspheres and preparation method and application thereof |
CN106880648A (en) * | 2015-12-16 | 2017-06-23 | 深圳翰宇药业股份有限公司 | A kind of pharmaceutical composition and preparation method thereof |
CN107184563A (en) * | 2017-05-31 | 2017-09-22 | 潘小平 | Micro-capsule, the preparation method and use of Physcion and its derivative |
CN107259577A (en) * | 2017-07-25 | 2017-10-20 | 李立风 | Probiotic gel grain and preparation method thereof |
CN107373029A (en) * | 2017-08-11 | 2017-11-24 | 中国农业科学院饲料研究所 | A kind of viable bacteria probiotics and its preparation method and application |
CN107674894A (en) * | 2017-09-21 | 2018-02-09 | 宿州天雨养殖(集团)有限公司 | A kind of preparation method that feed addictive microencapsulation bacterium peptide is produced using vinasse |
CN108174993A (en) * | 2018-01-30 | 2018-06-19 | 广西壮族自治区畜牧研究所 | It is a kind of using leaflet white popinac as egg feedstuff of raw material and preparation method thereof |
CN108244389A (en) * | 2018-01-30 | 2018-07-06 | 广西壮族自治区畜牧研究所 | It is a kind of using leaflet white popinac as broiler fodder of raw material and preparation method thereof |
CN108354181A (en) * | 2018-03-23 | 2018-08-03 | 刘洪明 | A kind of preparation method and system of three probiotic microcapsules |
CN108669298A (en) * | 2018-04-26 | 2018-10-19 | 九江牧威利元科技中心(普通合伙) | A kind of feeding probiotic microcapsule and its preparation method and application |
CN108669565A (en) * | 2018-04-25 | 2018-10-19 | 广东石油化工学院 | A kind of preparation method of microcapsules |
CN108719566A (en) * | 2018-05-14 | 2018-11-02 | 中玺(天津)枣业技术工程中心 | The sandwich soft sweets of jujube and processing method |
CN108740971A (en) * | 2018-05-14 | 2018-11-06 | 中玺(天津)枣业技术工程中心 | Green mustard jujube western-style food garnishes and production method |
CN108740248A (en) * | 2018-05-11 | 2018-11-06 | 中玺(天津)枣业技术工程中心 | Jujube haw sheet and production method |
CN108740244A (en) * | 2018-05-11 | 2018-11-06 | 中玺(天津)枣业技术工程中心 | Cartoon jujube sugar and processing method |
CN109170235A (en) * | 2018-08-01 | 2019-01-11 | 中国农业大学 | Probiotic microcapsule and the preparation method and application thereof |
CN109303166A (en) * | 2018-10-14 | 2019-02-05 | 浙江大学 | The preparation method of heat resistance lactobacillus micro-capsule |
WO2019031550A1 (en) * | 2017-08-09 | 2019-02-14 | ライオン株式会社 | Coating composition, coating film, and coating preparation and method for producing same |
CN109453207A (en) * | 2018-12-30 | 2019-03-12 | 广东南芯医疗科技有限公司 | A kind of probiotic double layer microcapsules, the preparation method and applications of selenizing sodium alginate and selenium chitosan cladding |
CN109527563A (en) * | 2018-12-24 | 2019-03-29 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of synbiotics microcapsule and its preparation method and application |
CN109528683A (en) * | 2018-12-13 | 2019-03-29 | 浙江华康药业股份有限公司 | A kind of arabinose fixed point release produces the regulation method of butyric to enteral |
CN109674061A (en) * | 2019-02-22 | 2019-04-26 | 山东探克生物科技股份有限公司 | A kind of prebiotics of double-layer microcapsule, probiotic composition and preparation method thereof |
CN109758434A (en) * | 2019-03-25 | 2019-05-17 | 成都医学院 | A kind of probiotics compound for treating inflammatory bowel disease |
CN110771898A (en) * | 2019-11-08 | 2020-02-11 | 厦门九和良方医药科技有限公司 | Probiotic microcapsule, preparation method and application thereof |
CN110946287A (en) * | 2019-11-05 | 2020-04-03 | 中国疾病预防控制中心营养与健康所 | Preparation method of probiotic-loaded microcapsule, product prepared from microcapsule and application of microcapsule |
CN110973357A (en) * | 2019-12-31 | 2020-04-10 | 宜兴市天石饲料有限公司 | Slow-release type compound acidifier and preparation method thereof |
CN111328803A (en) * | 2018-12-19 | 2020-06-26 | 北京理工大学 | Bacillus thuringiensis thallus microcapsule and preparation method and application thereof |
CN111529511A (en) * | 2020-04-28 | 2020-08-14 | 北京诺康达医药科技股份有限公司 | Probiotic microcapsule and preparation method and application thereof |
CN111588038A (en) * | 2020-05-11 | 2020-08-28 | 青岛明月海藻生物健康科技集团有限公司 | Seaweed enzyme probiotic microcapsule and preparation method thereof |
CN111631214A (en) * | 2020-07-02 | 2020-09-08 | 珠海格力电器股份有限公司 | Essential oil microcapsule, preparation method and application of essential oil microcapsule in humidifier |
CN111838677A (en) * | 2019-04-25 | 2020-10-30 | 北京慧明欧迈科技有限公司 | Culturable enteric bacteria microcapsule and preparation method thereof |
CN112244153A (en) * | 2020-08-29 | 2021-01-22 | 广州大北农农牧科技有限责任公司 | Preparation method of double-layer coated lactic acid bacteria microcapsule |
CN112262917A (en) * | 2020-10-26 | 2021-01-26 | 合肥五粮泰生物科技有限公司 | Preparation method of high-viable bacteria fermented feed |
CN112544977A (en) * | 2020-11-03 | 2021-03-26 | 国家粮食和物资储备局科学研究院 | Preparation method of multi-layer coated probiotic microcapsule |
CN113040174A (en) * | 2021-01-19 | 2021-06-29 | 深圳市芭田生态工程股份有限公司 | Microcapsule microbial inoculum and preparation method and application thereof |
CN113171358A (en) * | 2021-04-30 | 2021-07-27 | 吕豪 | Pet chewing gum based on alginate soft capsules |
CN113925129A (en) * | 2021-08-23 | 2022-01-14 | 北京同仁堂参茸中药制品有限公司 | Cereal blending powder and preparation method thereof |
CN115005448A (en) * | 2022-05-19 | 2022-09-06 | 成都凝创合炬生物科技集团有限公司 | Triple-coated probiotics and production method thereof |
CN115191611A (en) * | 2022-07-15 | 2022-10-18 | 江西中科农牧动物保健品有限公司 | Enteric-coated probiotic preparation and preparation method thereof |
CN115707389A (en) * | 2021-08-20 | 2023-02-21 | 江苏省农业科学院 | Phage gel and preparation method and application thereof |
CN115997934A (en) * | 2022-12-02 | 2023-04-25 | 安徽中微微元生物科技有限公司 | Probiotic nanoparticle slow-release preparation method |
CN116869970A (en) * | 2023-07-13 | 2023-10-13 | 安徽善和生物科技有限公司 | High-activity microcapsule probiotics and preparation method thereof |
-
2006
- 2006-12-04 CN CNB2006100705633A patent/CN100537759C/en not_active Expired - Fee Related
Cited By (88)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101249109B (en) * | 2008-03-14 | 2012-06-27 | 华侨大学 | Lactobacillus reuteri hollow micro-capsule and its preparing method |
CN101691568B (en) * | 2009-10-13 | 2012-02-08 | 哈尔滨美华生物技术股份有限公司 | Method of producing edible immobilized active probiotics |
CN103458709B (en) * | 2011-01-21 | 2017-10-03 | 凡赛尔医药股份有限公司 | Microencapsulation prebiotic substances and its manufacture method |
CN103458709A (en) * | 2011-01-21 | 2013-12-18 | 凡赛尔医药股份有限公司 | Microencapsulated probiotic substance and process of manufacture |
CN102198115B (en) * | 2011-05-19 | 2012-11-14 | 甘肃易源生物制药有限公司 | Enteric-coated micro-capsule prepared by mixing traditional Chinese medicine extract and probitics |
CN102198115A (en) * | 2011-05-19 | 2011-09-28 | 甘肃易源生物制药有限公司 | Enteric-coated micro-capsule prepared by mixing traditional Chinese medicine extract and probitics |
CN102334590B (en) * | 2011-08-10 | 2013-04-03 | 广东工业大学 | Method for recycling waste yogurt |
CN102334590A (en) * | 2011-08-10 | 2012-02-01 | 广东工业大学 | Method for recycling waste yogurt |
CN103357062B (en) * | 2012-03-26 | 2015-10-28 | 约泰实业股份有限公司 | Fiber water gel and preparation method thereof |
CN103357062A (en) * | 2012-03-26 | 2013-10-23 | 约泰实业股份有限公司 | Fibrous hydrogel and preparation method thereof |
CN103355656B (en) * | 2012-04-01 | 2016-01-20 | 中国科学院大连化学物理研究所 | A kind of probiotic microcapsule product and preparation and application thereof |
CN103355656A (en) * | 2012-04-01 | 2013-10-23 | 中国科学院大连化学物理研究所 | Probiotics microcapsule product and preparation and application thereof |
CN102613458A (en) * | 2012-04-16 | 2012-08-01 | 广东仙乐制药有限公司 | Probiotics soft capsule and preparation method thereof |
CN102613458B (en) * | 2012-04-16 | 2013-12-18 | 广东仙乐制药有限公司 | Probiotics soft capsule and preparation method thereof |
CN102640933A (en) * | 2012-04-17 | 2012-08-22 | 中国科学院烟台海岸带研究所 | Phycocyanin microcapsule and preparation method of phycocyanin microcapsule |
WO2013166681A1 (en) * | 2012-05-10 | 2013-11-14 | 聚和国际股份有限公司 | Composition for producing microcapsules and medicine made thereof |
CN104302279A (en) * | 2012-05-10 | 2015-01-21 | 聚和国际股份有限公司 | Composition for producing microcapsules and medicine made thereof |
CN102919542A (en) * | 2012-11-13 | 2013-02-13 | 西华大学 | Multistage-controlled-release probiotics microecological preparation composite feed for Silurus meridionalis and preparation method thereof |
CN102960600A (en) * | 2012-11-19 | 2013-03-13 | 陕西科技大学 | Method for preparing two-layer embedded bifidobacterium microcapsules |
CN103005463A (en) * | 2012-12-07 | 2013-04-03 | 北京百奥未来生物科技有限公司 | Low molecular collagen oral enteric microcapsule, preparation method thereof, and health drink |
CN103222539B (en) * | 2013-04-09 | 2015-05-06 | 思科福(北京)生物科技有限公司 | Preparation method of microbial pre-fermentation coating multilayer microcapsule |
CN103222539A (en) * | 2013-04-09 | 2013-07-31 | 魏永刚 | Preparation method of microbial pre-fermentation coating multilayer microcapsule |
CN103431379B (en) * | 2013-07-31 | 2015-11-25 | 吉林农业大学 | A kind of low power consuming is produced the lactic acid bacteria that can suspend and to be wet the production method of capsule |
CN103431379A (en) * | 2013-07-31 | 2013-12-11 | 吉林农业大学 | Method for producing suspended lactobacillus wet capsule with low energy consumption |
CN103704718B (en) * | 2013-12-23 | 2016-04-27 | 安徽大学 | Preparation method of bacillus subtilis microcapsule with high viable count in shelf life |
CN103704718A (en) * | 2013-12-23 | 2014-04-09 | 安徽大学 | Preparation method of bacillus subtilis microcapsule with high viable count in shelf life |
CN104887647B (en) * | 2014-03-08 | 2017-12-01 | 复旦大学 | Probiotic double layer microcapsules and preparation method thereof |
CN104887647A (en) * | 2014-03-08 | 2015-09-09 | 复旦大学 | Probiotics double-layered microcapsule and manufacturing method thereof |
CN104000025A (en) * | 2014-06-11 | 2014-08-27 | 北京昕地美饲料科技有限公司 | Method for making stabilized lactic acid bacteria product |
CN104000025B (en) * | 2014-06-11 | 2016-03-23 | 北京昕地美饲料科技有限公司 | A kind of preparation method of stabilisation lactic acid bacteria product |
CN104431370A (en) * | 2014-12-15 | 2015-03-25 | 南京优帆生物科技有限公司 | Efficient probiotics microcapsule as well as preparation method and application thereof |
CN104644612A (en) * | 2015-01-26 | 2015-05-27 | 天津科技大学 | Sodium polyacrylate-grafted and sodium alginate-embedded probiotic microcapsule and preparation method thereof |
CN104957254A (en) * | 2015-06-08 | 2015-10-07 | 内蒙古蒙牛乳业(集团)股份有限公司 | Probiotics gel particle, preparation method thereof and dairy containing probiotics gel particle |
CN104957254B (en) * | 2015-06-08 | 2018-10-16 | 内蒙古蒙牛乳业(集团)股份有限公司 | Probiotic gel particle, preparation method and the dairy products containing it |
CN104970368A (en) * | 2015-06-30 | 2015-10-14 | 安徽大学 | Preparation method of gastric acid-resistant bacillus licheniformis microcapsules with high survival rate |
CN106880648A (en) * | 2015-12-16 | 2017-06-23 | 深圳翰宇药业股份有限公司 | A kind of pharmaceutical composition and preparation method thereof |
CN105963344A (en) * | 2016-07-13 | 2016-09-28 | 中国科学院海洋研究所 | Fermented traditional Chinese medicine preparation for improving immunity of aquaculture animals and preparation method thereof |
CN106343578A (en) * | 2016-08-29 | 2017-01-25 | 河北然生物科技有限公司 | Enteric double-layer seamless solid probiotic microspheres and preparation method and application thereof |
CN107184563A (en) * | 2017-05-31 | 2017-09-22 | 潘小平 | Micro-capsule, the preparation method and use of Physcion and its derivative |
CN107259577A (en) * | 2017-07-25 | 2017-10-20 | 李立风 | Probiotic gel grain and preparation method thereof |
JP7111104B2 (en) | 2017-08-09 | 2022-08-02 | ライオン株式会社 | Coating composition, coating film, coating formulation and method for producing the same |
JPWO2019031550A1 (en) * | 2017-08-09 | 2020-09-03 | ライオン株式会社 | Coating composition, coating film, coating preparation and its manufacturing method |
WO2019031550A1 (en) * | 2017-08-09 | 2019-02-14 | ライオン株式会社 | Coating composition, coating film, and coating preparation and method for producing same |
CN107373029A (en) * | 2017-08-11 | 2017-11-24 | 中国农业科学院饲料研究所 | A kind of viable bacteria probiotics and its preparation method and application |
CN107373029B (en) * | 2017-08-11 | 2019-10-01 | 中国农业科学院饲料研究所 | A kind of viable bacteria probiotics and its preparation method and application |
CN107674894A (en) * | 2017-09-21 | 2018-02-09 | 宿州天雨养殖(集团)有限公司 | A kind of preparation method that feed addictive microencapsulation bacterium peptide is produced using vinasse |
CN108244389B (en) * | 2018-01-30 | 2020-09-01 | 广西壮族自治区畜牧研究所 | Broiler feed taking albizzia julibrissin as raw material and preparation method thereof |
CN108244389A (en) * | 2018-01-30 | 2018-07-06 | 广西壮族自治区畜牧研究所 | It is a kind of using leaflet white popinac as broiler fodder of raw material and preparation method thereof |
CN108174993A (en) * | 2018-01-30 | 2018-06-19 | 广西壮族自治区畜牧研究所 | It is a kind of using leaflet white popinac as egg feedstuff of raw material and preparation method thereof |
CN108354181A (en) * | 2018-03-23 | 2018-08-03 | 刘洪明 | A kind of preparation method and system of three probiotic microcapsules |
CN108669565A (en) * | 2018-04-25 | 2018-10-19 | 广东石油化工学院 | A kind of preparation method of microcapsules |
CN108669298A (en) * | 2018-04-26 | 2018-10-19 | 九江牧威利元科技中心(普通合伙) | A kind of feeding probiotic microcapsule and its preparation method and application |
CN108740248A (en) * | 2018-05-11 | 2018-11-06 | 中玺(天津)枣业技术工程中心 | Jujube haw sheet and production method |
CN108740244A (en) * | 2018-05-11 | 2018-11-06 | 中玺(天津)枣业技术工程中心 | Cartoon jujube sugar and processing method |
CN108719566A (en) * | 2018-05-14 | 2018-11-02 | 中玺(天津)枣业技术工程中心 | The sandwich soft sweets of jujube and processing method |
CN108740971A (en) * | 2018-05-14 | 2018-11-06 | 中玺(天津)枣业技术工程中心 | Green mustard jujube western-style food garnishes and production method |
CN109170235A (en) * | 2018-08-01 | 2019-01-11 | 中国农业大学 | Probiotic microcapsule and the preparation method and application thereof |
CN109303166A (en) * | 2018-10-14 | 2019-02-05 | 浙江大学 | The preparation method of heat resistance lactobacillus micro-capsule |
CN109528683A (en) * | 2018-12-13 | 2019-03-29 | 浙江华康药业股份有限公司 | A kind of arabinose fixed point release produces the regulation method of butyric to enteral |
CN111328803A (en) * | 2018-12-19 | 2020-06-26 | 北京理工大学 | Bacillus thuringiensis thallus microcapsule and preparation method and application thereof |
CN109527563A (en) * | 2018-12-24 | 2019-03-29 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of synbiotics microcapsule and its preparation method and application |
CN109453207A (en) * | 2018-12-30 | 2019-03-12 | 广东南芯医疗科技有限公司 | A kind of probiotic double layer microcapsules, the preparation method and applications of selenizing sodium alginate and selenium chitosan cladding |
CN109453207B (en) * | 2018-12-30 | 2021-12-31 | 广东南芯医疗科技有限公司 | Sodium selenylation alginate and chitosan selenylation coated probiotic double-layer microcapsule, preparation method and application thereof |
CN109674061A (en) * | 2019-02-22 | 2019-04-26 | 山东探克生物科技股份有限公司 | A kind of prebiotics of double-layer microcapsule, probiotic composition and preparation method thereof |
CN109758434A (en) * | 2019-03-25 | 2019-05-17 | 成都医学院 | A kind of probiotics compound for treating inflammatory bowel disease |
CN109758434B (en) * | 2019-03-25 | 2021-06-25 | 成都医学院 | Probiotic compound for treating inflammatory bowel disease |
CN111838677A (en) * | 2019-04-25 | 2020-10-30 | 北京慧明欧迈科技有限公司 | Culturable enteric bacteria microcapsule and preparation method thereof |
CN110946287A (en) * | 2019-11-05 | 2020-04-03 | 中国疾病预防控制中心营养与健康所 | Preparation method of probiotic-loaded microcapsule, product prepared from microcapsule and application of microcapsule |
CN110946287B (en) * | 2019-11-05 | 2022-07-08 | 中国疾病预防控制中心营养与健康所 | Preparation method of probiotic-loaded microcapsule, product prepared from microcapsule and application of microcapsule |
CN110771898A (en) * | 2019-11-08 | 2020-02-11 | 厦门九和良方医药科技有限公司 | Probiotic microcapsule, preparation method and application thereof |
CN110973357A (en) * | 2019-12-31 | 2020-04-10 | 宜兴市天石饲料有限公司 | Slow-release type compound acidifier and preparation method thereof |
CN111529511A (en) * | 2020-04-28 | 2020-08-14 | 北京诺康达医药科技股份有限公司 | Probiotic microcapsule and preparation method and application thereof |
CN111529511B (en) * | 2020-04-28 | 2022-04-15 | 北京诺葆营养科技有限公司 | Probiotic microcapsule and preparation method and application thereof |
CN111588038A (en) * | 2020-05-11 | 2020-08-28 | 青岛明月海藻生物健康科技集团有限公司 | Seaweed enzyme probiotic microcapsule and preparation method thereof |
CN111631214A (en) * | 2020-07-02 | 2020-09-08 | 珠海格力电器股份有限公司 | Essential oil microcapsule, preparation method and application of essential oil microcapsule in humidifier |
CN112244153A (en) * | 2020-08-29 | 2021-01-22 | 广州大北农农牧科技有限责任公司 | Preparation method of double-layer coated lactic acid bacteria microcapsule |
CN112262917A (en) * | 2020-10-26 | 2021-01-26 | 合肥五粮泰生物科技有限公司 | Preparation method of high-viable bacteria fermented feed |
CN112544977B (en) * | 2020-11-03 | 2022-02-18 | 国家粮食和物资储备局科学研究院 | Preparation method of multi-layer coated probiotic microcapsule |
CN112544977A (en) * | 2020-11-03 | 2021-03-26 | 国家粮食和物资储备局科学研究院 | Preparation method of multi-layer coated probiotic microcapsule |
CN113040174A (en) * | 2021-01-19 | 2021-06-29 | 深圳市芭田生态工程股份有限公司 | Microcapsule microbial inoculum and preparation method and application thereof |
CN113171358A (en) * | 2021-04-30 | 2021-07-27 | 吕豪 | Pet chewing gum based on alginate soft capsules |
CN115707389A (en) * | 2021-08-20 | 2023-02-21 | 江苏省农业科学院 | Phage gel and preparation method and application thereof |
CN113925129A (en) * | 2021-08-23 | 2022-01-14 | 北京同仁堂参茸中药制品有限公司 | Cereal blending powder and preparation method thereof |
CN115005448A (en) * | 2022-05-19 | 2022-09-06 | 成都凝创合炬生物科技集团有限公司 | Triple-coated probiotics and production method thereof |
CN115191611A (en) * | 2022-07-15 | 2022-10-18 | 江西中科农牧动物保健品有限公司 | Enteric-coated probiotic preparation and preparation method thereof |
CN115997934A (en) * | 2022-12-02 | 2023-04-25 | 安徽中微微元生物科技有限公司 | Probiotic nanoparticle slow-release preparation method |
CN116869970A (en) * | 2023-07-13 | 2023-10-13 | 安徽善和生物科技有限公司 | High-activity microcapsule probiotics and preparation method thereof |
CN116869970B (en) * | 2023-07-13 | 2024-01-26 | 安徽善和生物科技有限公司 | High-activity microcapsule probiotics and preparation method thereof |
Also Published As
Publication number | Publication date |
---|---|
CN100537759C (en) | 2009-09-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN100537759C (en) | Enteric-coated multilayer encapsulated probiotic microcapsule and preparation method thereof | |
Rodrigues et al. | Encapsulated probiotic cells: Relevant techniques, natural sources as encapsulating materials and food applications–A narrative review | |
Razavi et al. | Microencapsulating polymers for probiotics delivery systems: Preparation, characterization, and applications | |
Silva et al. | Symbiotic microencapsulation to enhance Lactobacillus acidophilus survival | |
CN109619593B (en) | Probiotic double-layer microcapsule and preparation method thereof | |
Cui et al. | Survival and stability of bifidobacteria loaded in alginate poly-l-lysine microparticles | |
CN101257892B (en) | Colonic delivery of adsorbents | |
ES2698384T3 (en) | Multilayer probiotic microcapsules | |
ES2957783T3 (en) | Protection of microbial cells against acid degradation | |
KR101370143B1 (en) | Probiotic storage and delivery | |
Zhu et al. | Biomaterial-based encapsulated probiotics for biomedical applications: Current status and future perspectives | |
Sandolo et al. | Encapsulation of Cwp84 into pectin beads for oral vaccination against Clostridium difficile | |
Huang et al. | Microencapsulation of probiotic lactobacilli with shellac as moisture barrier and to allow controlled release | |
Nezamdoost-Sani et al. | Alginate and derivatives hydrogels in encapsulation of probiotic bacteria: An updated review | |
Iqbal et al. | In-vitro GIT tolerance of microencapsulated Bifidobacterium bifidum ATCC 35914 using polysaccharide-protein matrix | |
CN102960600A (en) | Method for preparing two-layer embedded bifidobacterium microcapsules | |
CN102960599A (en) | Method for preparing bifidobacterium microcapsules containing prebiotics | |
Wu et al. | Evaluation of chitosan hydrochloride-alginate as enteric micro-probiotic-carrier with dual protective barriers | |
Nezamdoost-Sani et al. | A comprehensive review on the utilization of biopolymer hydrogels to encapsulate and protect probiotics in foods | |
Vimon et al. | Matrices-charges of agar-alginate crosslinked microcapsules via o/w microemulsion: A non-spore forming probiotic bacteria encapsulation system for extensive viability | |
CN104970368A (en) | Preparation method of gastric acid-resistant bacillus licheniformis microcapsules with high survival rate | |
Mohammad et al. | Biopolymeric encapsulation of probiotics for improved release properties in the gastrointestinal digestion system | |
Bakhtiyari et al. | Optimization of co-encapsulation of L. plantarum cells and Silybum marianum seed extract and evaluation of protective effect of extract on cells survival in simulated gastrointestinal fluids | |
RU2775231C1 (en) | Method for producing capsules based on bacterial cellulose hydrogels | |
Sreya et al. | Science and Technology of Alginates: A Review |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C17 | Cessation of patent right | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090909 Termination date: 20131204 |