CN103431379B - A kind of low power consuming is produced the lactic acid bacteria that can suspend and to be wet the production method of capsule - Google Patents
A kind of low power consuming is produced the lactic acid bacteria that can suspend and to be wet the production method of capsule Download PDFInfo
- Publication number
- CN103431379B CN103431379B CN201310337653.4A CN201310337653A CN103431379B CN 103431379 B CN103431379 B CN 103431379B CN 201310337653 A CN201310337653 A CN 201310337653A CN 103431379 B CN103431379 B CN 103431379B
- Authority
- CN
- China
- Prior art keywords
- lactic acid
- sodium alginate
- suspend
- acid bacteria
- rotating speed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Medicinal Preparation (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Manufacturing Of Micro-Capsules (AREA)
Abstract
The invention discloses a kind of low power consuming to produce the lactic acid bacteria that can suspend and to wet the production method of capsule, the method comprises the following steps: cultivate lactic acid bacteria, bacterium liquid is centrifugal, suspended bacteria mud; Bacterium mud and sodium alginate soln stirring and emulsifying prepare suspension; Calcium carbonate suspension and the emulsification of bacterium mud sodium alginate suspension centrifugal, collecting granules; The particle collected mixes with shitosan, emulsification collected by centrifugation particle; Sterilized water suspends and washs microcapsules.The present invention is on the basis of classical wall material sodium alginate and shitosan, by processing technology and use reagent to improve, low energy consumption, probiotic microcapsule particle is little, viable bacteria fatal rate is low; In addition; microcapsule granule prepared by the method is added in liquid food can form colloid; realize suspending and do not precipitate; do not affect food mouthfeel; probio height vigor can keep 90 ~ 120 days; after adding protective agent, high vigor can keep half a year more than, keeps microbial activity more than 1 year, can meet the requirement of Food Shelf-life completely.
Description
Technical field
The invention belongs to microcapsules preparation field, particularly relate to a kind of low power consuming and produce the lactic acid bacteria that can suspend and to wet the production method of capsule.
Background technology
Microcapsules technology (Microencapsulation) is the technology that micro substance is wrapped in thin polymer film, is the microdisplay package technology of a kind of store solids, liquid, gas.
Specifically, microcapsules technology refers to a certain object (core or interior phase) completely coated with various macromolecular compound continuous film (wall or foreign minister) that is natural or synthesis, and original chemical property of object at all be can't harm, then little by little by some outside stimulus or slow releasing function, the function of object is presented again in outside, or rely on the shielding action of cyst wall to play the effect of protection core, the diameter of microcapsules is generally 1 ~ 500 μm, the thickness of wall is 0.5 ~ 150 μm, develop the ultra micro capsule of particle diameter below 1 μm at present.
Microcapsule particle expands 0.25 ~ 1000 μm in some instances to.When Microcapsules Size is less than 5 μm, be not easy to collect because of Brownian movement aggravation; When particle diameter is greater than 300 μm, its skin-friction coefficient can decline suddenly and lose microcapsules effect.General capsule membrane wall thickness is 1-30 μm.32 μm and 180 μm mostly are in cosmetics.Ultra-thin-wall microcapsule membrane wall thickness is 0.01 μm.External microcapsules are for hiding in frost, NMF, lipstick, eye shadow, perfume, bath soap, face powder etc.Microcapsules can improve the stability of product, prevent the mutual interference between various component.
Wall material conventional in microcapsules technology has three classes: 1. natural macromolecular material, as: gelatin, Arabic gum, shellac, lac, starch, dextrin, wax, rosin, sodium alginate, zein, feature is nontoxic, stable, good film-forming property; 2. semi-synthetic macromolecular material, as: contracting methylcellulose, methylcellulose, ethyl cellulose, feature is that toxicity is little, and viscosity is large, and after salify, solubility increases, but facile hydrolysis, non-refractory, needs Extemporaneous; 3. full synthesized polymer material, as: polyethylene, polystyrene, polybutadiene, polypropylene, polyethers, polyureas, polyethylene glycol, polyvinyl alcohol, polyamide, polyacrylamide, polyurethane, polymethyl methacrylate, polyvinylpyrrolidone, epoxy resin, polysiloxanes, feature is good film-forming property, and chemical stability is good.
The method preparing microcapsules has Physical, spray drying process, spray chilling method, air suspension, vacuum vapor deposition, complex coacervation, how empty centrifugal process; Physical-chemical process, aqueous phase separation method, oil-phase separating method, limonene method, extrusion, orifice method, powder bed method; Chemical method, interfacial polymerization, situ aggregation method, molecule packing method, radiation packing method.
But the processing method power consumptions such as spray drying process, spray chilling method, air suspension are large, and have higher fatal rate to probio, form also particle comparatively large, difficulty suspends in a liquid.These reasons cause the cost of probiotic microcapsule to increase, and microcapsule granule has a negative impact to food proterties, limits the scope that probiotic microcapsule is applied in food.
Summary of the invention
The object of the embodiment of the present invention is to provide a kind of low power consuming to produce and can suspends that lactic acid bacteria wets the production method of capsule, be intended to solve the power consumption of existing method for processing microcapsules greatly, high to probio fatal rate, the problem of the food mouthfeel difference that microcapsule granule occurs after adding food.
The embodiment of the present invention is achieved in that a kind of low power consuming is produced the lactic acid bacteria that can suspend and to be wet the production method of capsule, and this preparation method comprises the following steps:
Cultivate lactic acid bacteria, bacterium liquid is centrifugal, suspended bacteria mud;
Bacterium mud and sodium alginate soln stirring and emulsifying prepare suspension;
Calcium carbonate suspension and the emulsification of bacterium mud sodium alginate suspension centrifugal, collecting granules;
The particle collected mixes with shitosan, emulsification collected by centrifugation particle;
Sterilized water suspends and washs microcapsules.
Further, the cultural method of lactic acid bacteria is: preparation MRS culture medium, by lactic acid bacteria according to 3% ~ 5% connect bacterium amount, be connected on 500mlMRS culture medium, 37 DEG C ~ 42 DEG C, cultivate 12 ~ 24h.
Further, bacterium liquid centrifuging and taking bacterium mud, rotating speed is at 5000 ~ 8000r/min.
Further, in bacterium mud, add volume ratio 50% ~ 80% glycerine 1 ~ 3ml after centrifugal acquisition bacterium mud, glycerine can replace with skimmed milk.
Further, the mass fraction of sodium alginate soln is 2% ~ 4%, and the mixed proportion of bacterium mud and sodium alginate soln is volume ratio 1: 5 ~ 10, and the stirring and emulsifying time is 15 ~ 30min, and rotating speed is 1000 ~ 5000r/min.
Further, the preparation method of calcium carbonate suspension is: calcium carbonate 300 ~ 400 order, be made into the suspension that mass fraction is 2 ~ 4%, adjust pH is to 7.5 ~ 8.5.
Further, calcium carbonate suspension and bacterium mud sodium alginate suspension emulsification times be 15 ~ 30min, rotating speed 1000 ~ 5000r/min; High-speed stirred, rotating speed is 1000 ~ 5000r/min, and the concentration adding hydrochloric acid solution is 0.01 ~ 0.1mol/L, is adjusted to PH4.5 ~ 5.5, continues stirring 10 ~ 15min after reaching pH, and really makes do not have calcium carbonate granule.
Further, the rotating speed of collected by centrifugation sodium alginate particle is 3000 ~ 5000r/min, and distilled water washing times is 2 ~ 3 times.
Further, the preparation method of chitosan particle is:
The sodium alginate particle collected is added in the chitosan solution of 1% ~ 3%, solution ph 3.0 ~ 4.0, high-speed stirred emulsification, and rotating speed 1000 ~ 5000r/min, 15 ~ 30min, prepare chitosan particle;
Collected by centrifugation particle, adds shitosan supernatant, suspended particulate, rotating speed 1000 ~ 5000r/min, adds 0.01 ~ 1mol/LNaOH solution adjust pH 6.0 ~ 7.0.
Further, sterilized water washing times is 3 times.
The invention provides low power consuming, to produce the wet production method of capsule of the lactic acid bacteria that can suspend be on the basis of classical wall material sodium alginate and shitosan, by processing technology and use reagent to improve, low energy consumption, probiotic microcapsule particle is little, viable bacteria fatal rate is low.The microcapsule granule diameter of the method processing technology is 10
-6~ 10
-8m, is added in liquid food and can forms colloid, realizes suspending not precipitating, and does not affect food mouthfeel.Probio height vigor can keep 90 ~ 120 days, and after adding protective agent, high vigor can keep half a year more than, keeps microbial activity more than 1 year, can meet the requirement of Food Shelf-life completely.
Accompanying drawing explanation
Fig. 1 is that low power consuming that the embodiment of the present invention provides is produced the lactic acid bacteria that can suspend and to be wet the flow chart of production method of capsule.
Detailed description of the invention
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Fig. 1 shows low power consuming provided by the invention and produces the lactic acid bacteria that can suspend and to wet the flow process of production method of capsule.For convenience of explanation, illustrate only part related to the present invention.
As shown in Figure 1, the low power consuming that the embodiment of the present invention provides is produced the wet production method of capsule of the lactic acid bacteria that can suspend and is comprised the following steps:
Cultivate lactic acid bacteria, bacterium liquid is centrifugal, suspended bacteria mud;
Bacterium mud and sodium alginate soln stirring and emulsifying prepare suspension;
Calcium carbonate suspension and the emulsification of bacterium mud sodium alginate suspension centrifugal, collecting granules;
The particle collected mixes with shitosan, emulsification collected by centrifugation particle;
Sterilized water suspends and washs microcapsules.
As a prioritization scheme of the embodiment of the present invention, the cultural method of lactic acid bacteria is: preparation MRS culture medium, by lactic acid bacteria according to 3% ~ 5% connect bacterium amount, be connected on 500mlMRS culture medium, 37 DEG C ~ 42 DEG C, cultivate 12 ~ 24h.
As a prioritization scheme of the embodiment of the present invention, bacterium liquid centrifuging and taking bacterium mud, rotating speed is at 5000 ~ 8000r/min.
As a prioritization scheme of the embodiment of the present invention, in bacterium mud, add volume ratio 50% ~ 80% glycerine 1 ~ 3ml after centrifugal acquisition bacterium mud, glycerine can replace with skimmed milk.
As a prioritization scheme of the embodiment of the present invention, the mass fraction of sodium alginate soln is 2% ~ 4%, and the mixed proportion of bacterium mud and sodium alginate soln is volume ratio 1: 5 ~ 10, and the stirring and emulsifying time is 15 ~ 30min, and rotating speed is 1000 ~ 5000r/min.
As a prioritization scheme of the embodiment of the present invention, the preparation method of calcium carbonate suspension is: calcium carbonate 300 ~ 400 order, and be made into the suspension that mass fraction is 2 ~ 4%, adjust pH is to 7.5 ~ 8.5.
As a prioritization scheme of the embodiment of the present invention, calcium carbonate suspension and bacterium mud sodium alginate suspension emulsification times are 15 ~ 30min, rotating speed 1000 ~ 5000r/min; High-speed stirred, rotating speed is 1000 ~ 5000r/min, and the concentration adding hydrochloric acid solution is 0.01 ~ 0.1mol/L, is adjusted to PH4.5 ~ 5.5, continues stirring 10 ~ 15min after reaching pH, and really makes do not have calcium carbonate granule.
As a prioritization scheme of the embodiment of the present invention, the rotating speed of collected by centrifugation sodium alginate particle is 3000 ~ 5000r/min, and distilled water washing times is 2 ~ 3 times.
As a prioritization scheme of the embodiment of the present invention, the preparation method of chitosan particle is:
The sodium alginate particle collected is added in the chitosan solution of 1% ~ 3%, solution ph 3.0 ~ 4.0, high-speed stirred emulsification, and rotating speed 1000 ~ 5000r/min, 15 ~ 30min, prepare chitosan particle;
Collected by centrifugation particle, adds shitosan supernatant, suspended particulate, rotating speed 1000 ~ 5000r/min, adds 0.01 ~ 1mol/LNaOH solution adjust pH 6.0 ~ 7.0.
As a prioritization scheme of the embodiment of the present invention, sterilized water washing times is 3 times.
Below in conjunction with drawings and the specific embodiments, application principle of the present invention is further described.
The low power consuming that the embodiment of the present invention provides is produced the wet production method of capsule of the lactic acid bacteria that can suspend and is comprised the following steps:
S101: cultivate lactic acid bacteria, bacterium liquid is centrifugal, suspended bacteria mud;
S102: bacterium mud and sodium alginate soln stirring and emulsifying prepare suspension;
S103: calcium carbonate suspension and the emulsification of bacterium mud sodium alginate suspension centrifugal, collecting granules;
S104: the particle of collection mixes with shitosan, emulsification collected by centrifugation particle;
S105: sterilized water suspends and washs microcapsules.
The low power consuming that the embodiment of the present invention provides is produced the wet production method of capsule of the lactic acid bacteria that can suspend and to be wet microcapsules for the not tally bacillus that can suspend, and with the not tally bacillus of two qi for core, produces the not tally wet microcapsules that can suspend.Concrete steps are:
1. the bacterium liquid getting 10 ~ 25ml is received 500ml and has been gone out in the MRS of bacterium, 37 DEG C of constant temperature culture 12 ~ 24h.
2. rotating speed 1000 ~ 5000r/min, centrifuging and taking bacterium mud.
3. in bacterium mud, add the glycerine that 2ml volume ratio is 50% ~ 80%, as bacteria protectant.
4. add 7 ~ 9ml physiological saline suspended bacteria mud.
5., under high-speed stirred, rotating speed 1000 ~ 5000r/min, joining mass fraction according to volume ratio 1: 5 is in the sodium alginate soln of 2% ~ 4%, emulsification 15 ~ 30min.With the NaOH solution adjust pH of 1mol/1 to 7.5 ~ 8.5.
6. 200 ~ 400 order calcium carbonate are made into the suspension that mass fraction is 2% ~ 4%, with the NaOH solution adjust pH of 0.01 ~ 1mol/1 to 7.5 ~ 8.5.Then slowly join in 5 sodium alginate solns obtained, stirring and emulsifying 15 ~ 30min, rotating speed 1000 ~ 5000r/min.
7. keep high-speed stirred, slowly add the hydrochloric acid solution of 0.01 ~ 0.1mol/L, formed to liquid particles, no longer include calcium carbonate powder in solution, pH value is 5.0, no longer raises in 5 ~ 10min.
8. collected by centrifugation particle, discards supernatant, rotating speed 3000 ~ 5000r/min.
9. shake scattered seed, and joining the mass fraction prepared is in 2% ~ 4% chitosan solution, emulsify at a high speed 20 ~ 30min, rotating speed 1000 ~ 5000r/min.
10. the made microcapsules of collected by centrifugation, after adding the suspension of a small amount of sterilized water, adjust pH is to 6.0 ~ 7.0.
With aseptic water washing microcapsules three times, soak with physiological saline, short-term 4 DEG C preservation; Or vacuum freeze-drying, long-term-80 DEG C of preservations.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (1)
1. low power consuming is produced the lactic acid bacteria that can suspend and to be wet the production method of capsule, it is characterized in that, described low power consuming is produced the lactic acid bacteria that can suspend and wet the production method of capsule with the not tally bacillus of two qi for core, and producing the not tally wet micro-glue concrete steps that can suspend is:
(1). the bacterium liquid getting 10 ~ 25ml is received 500ml and has been gone out in the MRS of bacterium, 37 DEG C of constant temperature culture 12 ~ 24h;
(2). rotating speed 1000 ~ 5000r/min, centrifuging and taking bacterium mud;
(3). in bacterium mud, add the glycerine that 2ml volume ratio is 50% ~ 80%, as bacteria protectant;
(4). add 7 ~ 9ml physiological saline suspended bacteria mud;
(5). under high-speed stirred, rotating speed 1000 ~ 5000r/min, joining mass fraction according to volume ratio 1: 5 is in the sodium alginate soln of 2% ~ 4%, emulsification 15 ~ 30min, with the NaOH solution adjust pH of 1mol/l to 7.5 ~ 8.5;
(6). 200 ~ 400 order calcium carbonate are made into the suspension that mass fraction is 2% ~ 4%, with the NaOH solution adjust pH of 0.01 ~ 1mol/l to 7.5 ~ 8.5; Then slowly join in 5 sodium alginate solns obtained, stirring and emulsifying 15 ~ 30min, rotating speed 1000 ~ 5000r/min;
(7). keep high-speed stirred, slowly add the hydrochloric acid solution of 0.01 ~ 0.1mol/L, formed to liquid particles, no longer include calcium carbonate powder in solution, pH value is 5.0, no longer raises in 5 ~ 10min;
(8). collected by centrifugation particle, discards supernatant, rotating speed 3000 ~ 5000r/min;
(9). shake scattered seed, and joining the mass fraction prepared is in 2% ~ 4% chitosan solution, emulsify at a high speed 20 ~ 30min, rotating speed 1000 ~ 5000r/min;
(10). the made microcapsules of collected by centrifugation, after adding the suspension of a small amount of sterilized water, adjust pH is to 6.0 ~ 7.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310337653.4A CN103431379B (en) | 2013-07-31 | 2013-07-31 | A kind of low power consuming is produced the lactic acid bacteria that can suspend and to be wet the production method of capsule |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310337653.4A CN103431379B (en) | 2013-07-31 | 2013-07-31 | A kind of low power consuming is produced the lactic acid bacteria that can suspend and to be wet the production method of capsule |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103431379A CN103431379A (en) | 2013-12-11 |
| CN103431379B true CN103431379B (en) | 2015-11-25 |
Family
ID=49685176
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310337653.4A Active CN103431379B (en) | 2013-07-31 | 2013-07-31 | A kind of low power consuming is produced the lactic acid bacteria that can suspend and to be wet the production method of capsule |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103431379B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109303166A (en) * | 2018-10-14 | 2019-02-05 | 浙江大学 | The preparation method of heat resistance lactobacillus micro-capsule |
| CN109453207B (en) * | 2018-12-30 | 2021-12-31 | 广东南芯医疗科技有限公司 | Sodium selenylation alginate and chitosan selenylation coated probiotic double-layer microcapsule, preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1969889A (en) * | 2006-12-04 | 2007-05-30 | 济南赛拜斯生物工程有限公司 | Enteric-coated multilayer encapsulated probiotic microcapsule and preparation method thereof |
| CN101401638A (en) * | 2008-10-17 | 2009-04-08 | 东北农业大学 | Method for preparing edible mushroom synbiotics microcapsule |
| CN101683604A (en) * | 2009-08-21 | 2010-03-31 | 黑龙江大学 | Method for preparing lactobacillus microcapsules |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100484480B1 (en) * | 2001-05-04 | 2005-04-20 | 정 호 김 | Encapsulation of Lactobacillus fermentum YL-3 |
-
2013
- 2013-07-31 CN CN201310337653.4A patent/CN103431379B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1969889A (en) * | 2006-12-04 | 2007-05-30 | 济南赛拜斯生物工程有限公司 | Enteric-coated multilayer encapsulated probiotic microcapsule and preparation method thereof |
| CN101401638A (en) * | 2008-10-17 | 2009-04-08 | 东北农业大学 | Method for preparing edible mushroom synbiotics microcapsule |
| CN101683604A (en) * | 2009-08-21 | 2010-03-31 | 黑龙江大学 | Method for preparing lactobacillus microcapsules |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103431379A (en) | 2013-12-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2013138291A (en) | MICROINCapsULATED PROBIOTIC SUBSTANCE AND METHOD OF ITS PRODUCTION | |
| CN106982825A (en) | A kind of antibacterial microcapsule and preparation method thereof and anti-bacterial packaging film | |
| CN101724163B (en) | Composite microsphere of chitosan derivative, preparing method and applications thereof | |
| CN103665398B (en) | The preparation method of fully biodegradable and biocompatible complex microsphere | |
| CN108752603A (en) | A kind of preparation method of starch base Pickering emulsion gels | |
| CN103431379B (en) | A kind of low power consuming is produced the lactic acid bacteria that can suspend and to be wet the production method of capsule | |
| CN105343883B (en) | The preparation method of the compound galapectite pipe microballoon of porous calcium carbonate | |
| CN107789332A (en) | A kind of calcium carbonate/calcium alginate compounded microballoon that adjustable drug release rate is prepared based on aqueous two-phase biomineralization technology | |
| CN106390175A (en) | Antibacterial carboxymethyl chitosan/nano zinc oxide composite microsphere and preparation method thereof | |
| CN106076214A (en) | A kind of calcium alginate microsphere preparation method with nucleocapsid structure | |
| CN105348548A (en) | Hydrogel microspheres based on glucan and preparation method thereof | |
| CN114766481B (en) | Pesticide microcapsule with high encapsulation efficiency and preparation method and application thereof | |
| CN111733531A (en) | Multifunctional mask and preparation method thereof | |
| CN106074684A (en) | Slow-releasing sachet and preparation method thereof | |
| CN104489237B (en) | A kind of modified rapeseed protein, microcapsules and preparation method | |
| CN105709671B (en) | A kind of preparation method of polyelectrolyte microcapsule | |
| CN104138471A (en) | Novel chitosan nanoparticles and preparation method thereof | |
| CN100569835C (en) | A kind of preparation method of hydroxypropyl chitosan nano particles | |
| CN104741618A (en) | Method for preparing platinum nanowires through helicoverpa armigera nuclear polyhedron extractives | |
| CN104151841A (en) | Multilayer hollow casein-based submicrometer composite microcapsule and preparation method thereof | |
| CN102771781A (en) | Preparation method of novel nano DNA nutrition powder | |
| CN110433741B (en) | Aromatic antibacterial microcapsule with carbon skeleton and preparation method thereof | |
| CN103497344A (en) | Nanogel for loading noble metal particles as well as preparation method and application thereof | |
| CN104801247B (en) | A kind of control release type yeast cells microcapsule product and preparation method thereof | |
| CN101933906B (en) | Preparation method of controlled-release drug-carried composite microsphere with small particle size and high mechanical strength |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |