CN108669565A - A kind of preparation method of microcapsules - Google Patents
A kind of preparation method of microcapsules Download PDFInfo
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- CN108669565A CN108669565A CN201810377215.3A CN201810377215A CN108669565A CN 108669565 A CN108669565 A CN 108669565A CN 201810377215 A CN201810377215 A CN 201810377215A CN 108669565 A CN108669565 A CN 108669565A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/03—Organic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a kind of preparation methods of microcapsules, include the following steps：MRS and YPD culture mediums are prepared first, are activated by Lactobacillus casei, and lactic acid bacteria expands culture and collects suspension, bacterium glue mixing is carried out, i.e., sodium alginate soln, oxygen scavenger, prebiotics are added into lactic acid bacteria suspension, and be uniformly mixed, immobilization 15min during microtitrimetry is added dropwise into calcium chloride solution, with filtered through gauze, chitosan is then used to impregnate 5min, after filtered through gauze, use salt acid soak 3min, filtered through gauze again finally uses brine, drying just to obtain finished product.The present invention is got up lactic acid bacteria embedding by two membranes, make lactic acid bacteria that still there is very high activity and survival rate after processing, storage and human consumption, after solving existing sour milk beverage through a long time storage, viable count fierce can decline, can more it lack in the lactic acid bacteria for reaching small intestine after the rugged environments such as the gastric juice of digestion, bile, it is difficult to the problem of playing effective health-care effect.
Description
Technical field
The present invention relates to microcapsules technology field, specially a kind of preparation method of microcapsules.
Background technology
Microcapsules are a kind of substances such as natural plant gum, polysaccharide, starch, protein, cellulose, polymer, wax and lipides
Solid, liquid, gas or bacterium etc. are wrapped the particle with core wall material structure.The size of microcapsule granule generally all exists
In 5-200 μ ms, it can material wrap, protect core material, keep the original color, smell and taste of core material, performance or bioactivity
Deng preventing the loss and destruction of nutriment, microcapsules are in fields such as medicine, food, cosmetics, agricultural, bioengineering at present
With extensive development prospect.With the research that deepens continuously to microcapsules, new microcapsules technology also constantly created and
Exploitation.
In addition, with the development of economy and people's living standards continue to improve, the exploitation of functional food increasingly by
To the attention of people.And can promote the intestinal flora ecological balance as the sour milk beverage containing profitable probliotics, become functional
Important a member in food.But by storing for a long time, viable count fierce can decline this beverage, be by human consumption
The lactic acid bacteria of small intestine is reached after the rugged environments such as gastric juice, the bile of system more to be lacked, it is difficult to play effective health-care effect, be
This, it is proposed that a kind of preparation method of microcapsules.
Invention content
The purpose of the present invention is to provide a kind of preparation methods of microcapsules, to solve mentioned above in the background art ask
Topic.
To achieve the above object, the present invention provides the following technical solutions：A kind of preparation method of microcapsules, including walk as follows
Suddenly：MRS and YPD culture mediums are prepared first, are activated by cheese lactic acid bacteria, after expanding culture and collecting lactic acid bacteria suspension, are carried out
Bacterium glue mixes, i.e., sodium alginate soln, oxygen scavenger, prebiotics is added into lactic acid bacteria suspension, and be uniformly mixed；Microtitrimetry
Then immobilization 15min in being added dropwise into calcium chloride solution uses chitosan to impregnate 5min with filtered through gauze, after filtered through gauze, make
With salt acid soak 3min, filtered through gauze, finally uses brine, drying just to obtain finished product again.
Preferably, it when the lactic acid bacteria suspension is prepared needs that first centrifuge tube is carried out to boil sterilization, in sterile working environment
It is middle that the lactic acid bacteria of Liquid Culture is poured into centrifuge tube, it is centrifuged, removes layer bacterium mud in the test tube of sterile saline, be made into 5*
The bacteria suspension of 109cfu/g, manufacturing process require stringent sterile working, and the additive that is added, raw material will be through before addition
Cross pasteurization or sterilizing.
Preferably, the bacterium glue mixing is needed according to 3：1 mass ratio is by a concentration of 3% sodium alginate soln and bacterium
Suspension mixes, the oxygen scavenger for being stirring evenly and then adding into the prebiotics for accounting for mixed liquor gross mass 8% and accounting for mixed liquor gross mass 0.14%,
Wherein, in prebiotics, synanthrin 3%, oligofructose 2.5%, oligomeric lactose 2.5%, in oxygen scavenger, cysteine hydrochloride 0.05%,
Sodium ascorbate 0.09%.
Preferably, the immobilization is needed to be dropped in the bacterium glue being mixed to get using microtitrimetry and be gone out by Pasteur
Bacterium, cooling 1.5% calcium chloride solution in be fixed, filtered after fixed 15min, obtain the core material of microcapsules.
Preferably, the microtitrimetry needs to utilize syringe（Internal diameter 0.5mm）By the drop of bacterium glue drop by drop into
Calcium chloride solution uses injector arrangement when needing a large amount of making products.
Preferably, the chitosan solution when immersion be by fingernail element by alkalization etc. obtain after reactions can be with acid
Water-soluble chitosan of reaction, chitosan and sodium alginate attract to form polyelectrolyte film by positive and negative charge when immersion.
Preferably, it is washed in such a way that physiological saline thread is mild in the washing process, must not stir or acutely shake
It swings.
There are mainly two types of methods for current Micro-Encapsulation Technique, and one is what Sheu and Marshall was proposed to be wrapped with oil
Water system embeds the chemical method of bacterium, referred to as emulsion process；Another kind is being set with syringe or needle nozzle for Sehultz propositions
It is standby liquid to be squeezed out the method being fixed, referred to as extrusion.The descendants such as Wang Shunyu, D.A. Russell, R.P. Ross exist
A large amount of research and innovation is carried out on the basis of both methods, but major part only rests on the basis of one layer of embedding.And it uses
Two layers of embedding of extrusion progress mainly have Paulraj Kanmani, R. Satish Kumar, Qi Wei, Yang Zeyu, Du Lianxiang
Et al., but they attract to form the principle of transparent polyelectrolyte film just with the positive and negative charge to chitosan and sodium alginate
Carry out the second coating film.The present invention innovative point mainly alkalization processing first is carried out to chitosan, water can be dissolved in, with sea
After mosanom forms polyelectrolyte film, acid adding, chitosan can occur chemical reaction with acid at once and form one layer of white chitosan
Polymeric membrane keeps film even closer, and acid resistance is stronger.
The beneficial effects of the invention are as follows：The present invention can prevent product is rotten, rotten, mouldy to remove by adding oxygen scavenger
It is smelly, prebiotics are added, can be grown with stimulating lactic acid bacteria, increase Lactobacillus Survival, using the chitosan and alginic acid of high concentration
Sodium attracts to form polyelectrolyte film by positive and negative charge, then when by being impregnated in hydrochloric acid, and the shell of hydrochloric acid and surface of microcapsule is poly-
Sugar reaction generates a kind of film of the white of energy stomach juice-resistant, realizes second layer embedding, makes lactic acid bacteria in processing, storage and human consumption
Still there is very high activity and survival rate afterwards, existing sour milk beverage can be solved and stored for a long time, under viable count meeting is fierce
Drop can more be lacked in the lactic acid bacteria for reaching small intestine after the rugged environments such as the gastric juice of digestion, bile, it is difficult to rise
The problem of to effective health-care effect.
Specific implementation mode
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described embodiment
Only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
The every other embodiment that art personnel are obtained without making creative work belongs to the model that the present invention protects
It encloses.
The present invention provides a kind of technical solution：A kind of preparation method of microcapsules, includes the following steps：MRS is prepared first
It with YPD culture mediums, is activated by cheese lactic acid bacteria, after expanding culture and collecting lactic acid bacteria suspension, carries out bacterium glue mixing, i.e., to outstanding
Sodium alginate soln, oxygen scavenger, prebiotics are added in liquid, and are uniformly mixed；Microtitrimetry is solid in being added dropwise into calcium chloride solution
Surely change 15min, with filtered through gauze, chitosan is then used to impregnate 5min, after filtered through gauze, using salt acid soak 3min, again
Filtered through gauze finally uses brine, drying just to obtain finished product.
Embodiment one：
MRS and YPD culture mediums are prepared first, are activated by cheese lactic acid bacteria, and lactic acid bacteria expands culture and collects suspension, carries out bacterium
Glue mixes, i.e., sodium alginate soln, oxygen scavenger, prebiotics is added into suspension, and be uniformly mixed, and then uses calcium chloride solution
Filtered through gauze is used after immobilization 15min, is used filtered through gauze after then chitosan being used to impregnate 5min, is then used salt acid soak
It is filtered after 3min, finally uses brine, finished product is obtained by dry drying after washing.
Embodiment two：
MRS and YPD culture mediums are prepared first, are activated by cheese lactic acid bacteria, and lactic acid bacteria expands culture and collects suspension, carries out bacterium
Glue mixes, i.e., sodium alginate soln, oxygen scavenger, prebiotics is added into suspension, and be uniformly mixed；Then calcium chloride solution is used
Filtered through gauze is used after immobilization 15min, is used filtered through gauze after then chitosan being used to impregnate 5min, is then used salt acid soak
It is filtered after 3min, finally uses brine, finished product is obtained by dry drying after washing；When wherein lactic acid bacteria suspension is prepared
It needs that first centrifuge tube is carried out to boil sterilization, the lactic acid bacteria of Liquid Culture is poured into centrifuge tube in sterile working environment, carry out
Centrifugation removes layer bacterium mud in the test tube of sterile saline, is made into the bacteria suspension of 5*109cfu/g, manufacturing process requires stringent
Sterile working, the additive added, raw material will pass through pasteurization or sterilizing before addition.
Embodiment three：
MRS and YPD culture mediums are prepared first, are activated by cheese lactic acid bacteria, and lactic acid bacteria expands culture and collects suspension, carries out bacterium
Glue mixes, i.e., sodium alginate soln, xanthans, oxygen scavenger, prebiotics, dextrin is added into suspension, and be uniformly mixed, then makes
With filtered through gauze is used after calcium chloride solution immobilization 15min, filtered through gauze is used after then chitosan being used to impregnate 5min, is then made
With being filtered after salt acid soak 3min, brine is finally used, finished product is obtained by dry drying after washing；Wherein lactic acid bacteria
Suspension prepare when need first centrifuge tube is carried out to boil sterilization, in sterile working environment by the lactic acid bacteria of Liquid Culture pour into from
Heart pipe, is centrifuged, and is removed layer bacterium mud in the test tube of sterile saline, is made into the bacteria suspension of 5*109cfu/g, manufacturing process
It is required that stringent sterile working, the additive added, raw material will pass through pasteurization or sterilizing before addition；Wherein bacterium glue
Mixing is needed according to 3：1 ratio mixes 3% sodium alginate soln with bacteria suspension, is stirring evenly and then adding into that account for mixed liquor total
The prebiotics of quality 8% and the oxygen scavenger for accounting for mixed liquor gross mass 0.14%, wherein in prebiotics, synanthrin 3%, oligofructose
2.5%, oligomeric lactose 2.5%, in oxygen scavenger, cysteine hydrochloride 0.05%, sodium ascorbate 0.09%；Immobilization needs to utilize
Microtitrimetry by the bacterium glue being mixed to get drop in by pasteurization, cooling 1.5% calcium chloride solution in consolidated
It is fixed, it is filtered after fixed 15min, obtains the core material of microcapsules.
Example IV：
MRS and YPD culture mediums are prepared first, are activated by cheese lactic acid bacteria, and lactic acid bacteria expands culture and collects suspension, carries out bacterium
Glue mixes, i.e., sodium alginate soln, oxygen scavenger, prebiotics is added into suspension, and be uniformly mixed；Then calcium chloride solution is used
Filtered through gauze is used after immobilization 15min, is used filtered through gauze after then chitosan being used to impregnate 5min, is then used salt acid soak
It is filtered after 3min, finally uses brine, finished product is obtained by dry drying after washing；When wherein lactic acid bacteria suspension is prepared
It needs that first centrifuge tube is carried out to boil sterilization, the lactic acid bacteria of Liquid Culture is poured into centrifuge tube in sterile working environment, carry out
Centrifugation, removes layer bacterium mud in the test tube of sterile saline, is made into the bacteria suspension of about 5*109cfu/g, and manufacturing process requires tight
The sterile working of lattice, the additive added, raw material will pass through pasteurization or sterilizing before addition；Wherein bacterium glue mixing needs
It will be according to 3：1 ratio mixes 3% sodium alginate soln with bacteria suspension, is stirring evenly and then adding into and accounts for mixed liquor gross mass 8%
Prebiotics and account for the oxygen scavenger of mixed liquor gross mass 0.14%, wherein in prebiotics, synanthrin 3%, oligofructose 2.5% is oligomeric
Lactose 2.5%, in oxygen scavenger, cysteine hydrochloride 0.05%, sodium ascorbate 0.09%；Immobilization needs to utilize microtitration
Method by the bacterium glue being mixed to get drop in by pasteurization, cooling 1.5% calcium chloride solution in be fixed, in fixation
It is filtered after 15min, obtains the core material of microcapsules；Microtitrimetry needs to utilize syringe（Internal diameter 0.5mm）Bacterium glue one is dripped
The drop of one drop uses injector arrangement into calcium chloride solution when needing a large amount of making products；Chitosan solution when immersion be by
The water-soluble chitosan that can be reacted with acid that fingernail element is obtained by alkalization etc. after reactions, chitosan and seaweed when immersion
Sour sodium attracts to form polyelectrolyte film by positive and negative charge；It is washed in such a way that physiological saline thread is mild in washing process,
It must not stir or acutely shake.
The present invention can prevent rotten, rotten, the mouldy deodorization of product, add prebiotics, can pierce by adding oxygen scavenger
Swash lactobacter growth, increases Lactobacillus Survival, shape is attracted by positive and negative charge using the chitosan and sodium alginate of high concentration
At polyelectrolyte film, first coating is realized, when by being impregnated in hydrochloric acid, the chitosan reaction of hydrochloric acid and surface of microcapsule is given birth to
At a kind of film of the white of energy stomach juice-resistant, realizes second layer embedding, lactic acid bacteria is made still to have after processing, storage and human consumption
There are very high activity and survival rate, solve existing sour milk beverage and store for a long time, viable count fierce can decline, through remarkable
The lactic acid bacteria of small intestine is reached after the rugged environments such as gastric juice, the bile of body digestive system more to be lacked, it is difficult to play effective health care
The problem of effect.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (9)
1. a kind of preparation method of microcapsules, it is characterised in that：Include the following steps：MRS and YPD culture mediums, warp are prepared first
Overdrying junket lactic acid bacteria activates, and after expanding culture and collecting lactic acid bacteria suspension, carries out bacterium glue mixing, i.e., is added into lactic acid bacteria suspension
Sodium alginate soln, oxygen scavenger, prebiotics, and be uniformly mixed；Immobilization during microtitrimetry is added dropwise into calcium chloride solution
Then 15min uses chitosan to impregnate 5min with filtered through gauze, after filtered through gauze, using salt acid soak 3min, and gauze again
Filtering finally uses brine, drying just to obtain finished product.
2. a kind of preparation method of microcapsules according to claim 1, it is characterised in that：When the lactic acid bacteria suspension is prepared
It needs that first centrifuge tube is carried out to boil sterilization, the lactic acid bacteria of Liquid Culture is poured into centrifuge tube in sterile working environment, carry out
Centrifugation, removes layer bacterium mud in the test tube of sterile saline, is made into 5*109The bacteria suspension of cfu/g, manufacturing process require stringent
Sterile working, the additive added, raw material will pass through pasteurization or sterilizing in addition to bacteria suspension before addition.
3. a kind of preparation method of microcapsules according to claim 1, it is characterised in that：Bacterium glue mixing need according to
3：1 mass ratio mixes a concentration of 3% sodium alginate soln with bacteria suspension, is stirring evenly and then adding into and accounts for the total matter of mixed liquor
The prebiotics of amount 8% and the oxygen scavenger for accounting for mixed liquor gross mass 0.14%, wherein in prebiotics, synanthrin 3%, oligofructose 2.5%,
Oligomeric lactose 2.5%, in oxygen scavenger, cysteine hydrochloride 0.05%, sodium ascorbate 0.09%.
4. a kind of preparation method of microcapsules according to claim 1, it is characterised in that：The immobilization is needed using micro-
Amount titration by the bacterium glue being mixed to get drop in by pasteurization, cooling 1.5% calcium chloride solution in be fixed,
It is filtered after fixed 15min, obtains the core material of microcapsules.
5. a kind of preparation method of microcapsules according to claim 4, it is characterised in that：The microtitrimetry needs profit
With the syringe of internal diameter 0.5mm by bacterium glue drop drop by drop into calcium chloride solution.
6. a kind of preparation method of microcapsules according to claim 1, it is characterised in that：The chitosan solution refers to
The water-soluble chitosan that can be reacted with acid that chitin obtains after the reactions such as alkalization.
7. a kind of preparation method of microcapsules according to claim 1, it is characterised in that：Chitosan and sea when the immersion
Mosanom attracts to form polyelectrolyte film by positive and negative charge, makes chitosan uniform adsorption in core surfaces.
8. a kind of preparation method of microcapsules according to claim 1, it is characterised in that：The acid soak process is shell
Glycan is reacted with acid generates one layer of white polymeric membrane, the structure and resistance to acid and alkali of reinforced membrane.
9. a kind of preparation method of microcapsules according to claim 1, it is characterised in that：Using life in the washing process
The mild mode of reason brine thread is washed, and must not be stirred or acutely be shaken.
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Cited By (6)
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| CN110105145A (en) * | 2019-06-18 | 2019-08-09 | 贾臻 | A kind of preparation and its manufacturing method improving agricultural microorganism survival ability in different environments |
| WO2021082382A1 (en) * | 2018-11-06 | 2021-05-06 | 内蒙古蒙牛乳业（集团）股份有限公司 | Microcapsule, preparation method and application thereof |
| CN112915059A (en) * | 2021-02-03 | 2021-06-08 | 东阿阿胶股份有限公司 | Moisture-retaining flaky microcapsule containing donkey-hide gelatin and preparation method |
| CN112956698A (en) * | 2021-03-26 | 2021-06-15 | 四川农业大学 | Probiotics microcapsule embedded blasting bead and preparation method thereof |
| WO2022188335A1 (en) * | 2021-03-10 | 2022-09-15 | 中农宠物营养研究院(江苏)有限公司 | Chitosan-fe-coating-based synbiotic microcapsule capable of resisting gastric acid and realizing targeted release in intestinal tract, and preparation method therefor |
| RU2781792C1 (en) * | 2021-05-13 | 2022-10-18 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Курская государственная сельскохозяйственная академия имени И.И. Иванова" | Method for producing microcapsules of vetom 1 probiotic |
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