DE202013103204U1 - Preparation containing a probiotic culture - Google Patents

Abstract

A preparation for use in the food industry, pharmaceutical industry, human or veterinary medicine or cosmetics, characterized in that it comprises a probiotic culture in the form of a single or multiple probiotic in the amount of 1 x 10 3 to 1 x 10 14 CFU per 1 g of the preparation, and a carrier in the amount of 0.001 to 99 wt.%, wherein the probiotic culture is at least partially in the form of a biofilm adhered to the carrier.

Description

Technical part

The present invention relates to a preparation containing a probiotic culture in the form of a single or multiple probiotic and a carrier, the probiotic culture being at least partially in the form of a biofilm adhered to the carrier. Also described is a process for preparing the preparation and the use thereof in food supplements, special foods, foods, beverages, medicines, health products, cosmetic and hygienic products, animal feed products and probiotics containing supplements intended for human and veterinary use.

State of the art

The basic ability of microorganisms to form communities adherent to a solid surface has been described in oceanic microorganisms as early as the beginning of the twentieth century ( Zobel et al., 1935 ). It turns out that for most microorganisms such lifestyle is their basal way of life and that such microorganisms can occur in the natural environment, on the bodies of other living organisms or on artificial surfaces in the form of specific adherent communities. Such sophisticated, highly organized and usually multi-layered communities of microorganisms that adhere to biological or artificial surfaces and are enveloped by an extracellular polymer matrix have later been given the designation Biofilm ( Costerton et al., 1985 ).

Thanks to the interaction and communication among the individual cells, the biofilm develops as a relatively complex structure, which has some hints of a circulatory system and which allows for the nutrition and derivation of metabolites in an entire stratum. In the natural environment, one finds mostly biofilms formed predominantly of mixed bacterial communities, while a biofilm formed by a single bacterial strain is relatively often described more in the context of clinical practice. However, a bacterial community has considerable heterogeneity, even in the case of a single-stem biofilm.

The prerequisite for the formation of a biofilm is the ability of bacterial cells, on surfaces of various kinds, such as surfaces of living tissues, various introduced into the organisms medical aids and implants, water pipes, appropriately selected carriers in biotechnological cultivation processes o. The like. In the In the first phase of biofilm formation, during which the adhesion develops, the most important factors play the most important role in facilitating or at least facilitating adhesion. In addition to the physico-chemical properties of the adhesive surface, including the presence of adherent, adhesion-facilitating macromolecules (mostly proteins such as fibrin and fibronectin), this is also the presence of the factors which determine the adhesiveness of the microorganism in question, in particular around the layer of a polymeric extracellular matrix (Donlan, 2001), around the fimbriae as well as the receptors present on the surfaces of the microorganisms.

After adherence of the microorganisms to the surface of the carrier, the phase of accumulation and maturation of the biofilm, referred to as maturation, follows. It stimulates the expression of the genes involved in the formation of the complex structure of the biofilm, in particular the formation of the polymeric extracellular matrix, and alters the physiology of the bacterial cells. The bacteria aggregate, multiply and thus form the sgn. Microcolonies ( Costerton JW 1999, Stoodley et al., 2002 ). In contrast to the term "colony", which in microbiology refers to a group of microbes formed by dividing a single mother cell, the microcolonies resulting from aggregation of the bacteria can also be formed by several microorganismic strains.

During this phase of the bacteria is a relatively large amount of extracellular substance, a sgn. Slime, produced with various chemical composition. This substance is composed for the most part of both substituted and unsubstituted polysaccharides, which are also referred to as extracellular polymeric substances (EPS). It may also contain proteins, nucleic acids or phospholipids. The EPS, together with the microcolonies consisting of bacterial cells, constitutes the fundamental structural component of the biofilm, the properties of which strongly influence it. Their accumulation in the vicinity of the microcolonies causes the gradual formation of a mature layer of the biofilm. The achievement of the critical amount of the cells as well as the biofilm mass finally leads to the transition into the next phase, namely into the dispersion phase, during which both the individual cells enveloped by the EPS and their clusters detach from the biofilm in order to reproduce further ( Donlan, 2001). Among the most important factors involved in this phase are, in addition to the mechanical effects of the environment (force of water flow) and the availability of nutrients and oxygen, the concentration of the metabolites and the regulatory systems, especially the system called quorum sensing , The microorganisms growing in the form of a biofilm differ from their planktonic forms by the transcription of different genes and thus also by their physiological properties, so that a certain biofilm phenotype "arises ( O'Toole et al., 2000; Donlan et Costerton 2002 ). In addition, the environment in a biofilm is not homogeneous and the bacteria are thus exposed to the influences of different conditions. These conditions may include varying concentrations of signaling molecules, nutrients, oxygen, and by-produced metabolites in the individual layers of the biofilm. This causes considerable heterogeneity, even within the bacterial community in the biofilm. Also contributing to this heterogeneity is the fact that the microcolonies result from the aggregation of the "biofilm phenotype" cells and the "planktonic" phenotype-bearing cells ( Rickard et al., 2003 ).

The structure as well as the shape of the biofilm are very variable and depend on both the actual biofilm-forming microbial strains and the environmental conditions, in particular on the availability of nutrients and oxygen, on the pH, on the osmotic pressure or hydrodynamic ratios (Davey et O'Toole, 2000). In a nutrient-rich environment, the biofilm forms in a strong relatively homogeneous layer, often without the formation of channels. On the contrary, in a poor nutrient environment, the biofilm is formed either from a low mosaic structure or from spongy structures, the latter having well developed channels and pores with which the deeper layers of the biofilm provide nutrients and oxygen supplied and can be freed of metabolites ( Costerton et al., 1995 ; Costerton et al., 1999 . Stoodley et al., 2002 ).

Growth in the form of a biofilm is beneficial to the microorganisms. Unlike bacteria growing in planktonic form, growth in the form of a biofilm provides the necessary protection to the bacterial cells and ensures the maintenance of a certain level of homeostasis. The resulting biofilm layer, as well as the extracellular polymeric substances that envelop the cells, provide a barrier that isolates the bacteria from the surrounding environment. The cells embedded in the biofilm thus have, for example, a higher resistance to toxic substances, UV radiation, mechanical damage, bacteriophages or predators. In a human or animal body, they better resist the effects of the immune system or antibiotics.

The cells embedded in the biofilm work metabolically together and show a higher level of genetic change ( Costerton et al., 1995; O'Tooley et al., 2000 ). Moreover, thanks to relatively close contact between the individual cells, there is an intense change in the genetic information, in particular with regard to the conjugation and transfer of plasmids. In this way, among the individual communities present in the biofilm, it is possible, for example, to spread relatively rapidly, for example, those plasmids which transmit the genes which code for resistance to antibiotics, or the like. Hausner et al., 1999 ).

Today, there are modern combined systems in which the combination of the growth of the bacteria in suspended form and the growth of the cultures on the immersed in an activating solution (with which a biological filter can be filled) or on that in a fluidized bed (which consists of polyurethane foam , which may be in the form of spheres), is applied to housed solid supports. Such systems are used for example in water treatment plants. A great advantage of such systems is the accumulation of a suspended culture as well as a biofilm culture in a reactor. This fact allows a significant increase in the supply of biomass in the system, which can also result in an increase in the capacity of the relevant water treatment plant. The diversity of biological cultures growing in such reactors can also make the processes of biological nitrification and denitrification considerably more effective, stabilize the functioning of the system, and in many cases also solve the problem associated with the fibrous swelling of the activated sludge. In the context of food biotechnologies, such carriers are used primarily in the production of vinegar. Bacteria used in vinegar builders must have good adhesion to the material in question. With this property, the formation of mucus (cuticle) is dependent, whose chemical basis is formed mainly by polysaccharides. Carriers in the form of fillers (such as cork, pumice) are also known and lately silicate-based supports have also begun to find application. The basic prerequisite for the proper functioning of a vinegar maker is the achievement of the greatest possible contact between Oxygen and the substrate containing the bacteria.

In the field of functional foods, dietary supplements and remedies containing probiotic cultures, the method described above has not been used heretofore. The invention makes it possible to see the probiotic preparations of the new generation from an innovative point of view. So far, attention has been focused only on the adhesion capabilities of the probiotic bacteria in relation to the gut epithelium and on the formation of a biofilm under the physiological conditions. The problem of preparing probiotic cultures in a biofilm structure already during the actual process of producing an active substance for dietary supplements, remedies, cosmetic products, health products, food, etc., has not been treated intensively.

Subject of the invention

For the purposes of the present invention, certain terms are used in the text, which are defined as follows:

The term biofilm is understood to mean a structure consisting of single or multi-layered groups of bacterial cells of at least one bacterial strain, the bacteria being enshrined in an amorphous extracellular material composed of exopolysaccharides (EPS), in particular of exopolysaccharides of bacterial origin which causes a firm adhesion between the cells and the surface of the carrier as well as between the adjacent cells.

The bacterial bacteria in the biofilm are characterized by an increased resistance to the effects of the external environment compared to the suspended bacteria, which in practice positively influences the stability of the product and its biological activity. This is due to the fact that the bacteria occurring in living systems form a biofilm structure by default.

The term carrier is understood here to mean a substance of organic or inorganic type which is used as a substrate for the cultivation of bacteria.

It is therefore an object of the present invention to provide an improved preparation containing a probiotic culture or several probiotic cultures. The preparation according to the invention is said to have more favorable properties in comparison to existing preparations, in particular with regard to its effectiveness and its resistance to external influences.

The invention relates to a preparation for use in the food industry, pharmaceutical industry, human or veterinary medicine or cosmetics, which comprises a probiotic culture in the form of a single or multiple probiotic in the amount of 1 × 10 ³ to 1 × 10 ¹⁴ CFU per 1 g and a carrier in the amount of 0.001 to 99 wt.%, Wherein the probiotic culture is at least partially in the form of a biofilm adhered to the carrier. The probiotic culture may be selected from the group comprising the genera Lactobacillus, Bifidobacterium, Streptococcus, Sacharomyces, or other human or animal beneficial or natural constituent of human or animal microflora forming bacteria or yeasts, or mixtures thereof , The carrier may be selected from the group consisting of oligosaccharides and polysaccharides such as microcrystalline cellulose (MCC), pectin, inulin, dextrin, maltodextrin or glycogen, corn or potato starch and other starches, proteins of microbial, plant or animal origin, crystals of various mineral substances , HMC, nanotextiles, FOS, GOS and other physiologically acceptable carriers or mixtures thereof, which may be included in the preparation in the minimum amount of 1% by weight. Advantageously, the preparation contains the probiotic culture in the amount of 1 × 10 ⁷ CFU / g, wherein 0.1 to 100% by weight of the probiotic culture is in the form of a biofilm. The probiotic culture should not be contaminated.

The preparation may further contain vitamins, minerals, vegetable extracts and other physiologically beneficial substances in the maximum amount of 99% by weight.

The preparation according to the invention can be present for example in the form of a tablet, a capsule, a powder or a granulate.

A process for the production of the preparation according to the invention is presented in which a culture medium is cultivated with a carrier and an inoculation solution containing the probiotic culture at a temperature of 20 to 40 ° C. until at least 5% of the carrier, advantageously 10% of the carrier, more preferably 20% of the carrier, and more preferably 100% of the carrier is covered with the probiotic culture, and then the resulting mixture is thickened and dried. The stated percentage values of the coverage of the carrier, however, should by no means limit the scope of the invention and the Cultivation process can thus be stopped even when 30%, 40% or 50% to 100% of the carrier are covered with the probiotic culture. The percentage coverage means that at least 5% of the entire surface of the support is covered with the biofilm (for example, if the support is in the form of spheres then at least 5% of the total number of spheres should be covered with the biofilm). In an advantageous embodiment of the method, the culture medium is first sterilized together with the carrier, then the inoculation solution is added to the medium, and then the resulting mixture is cultured at a temperature of 30 to 40 ° C. for 8 to 48 hours. Finally, the mixture is thickened and dried.

The culture medium which can be used in the process may be selected from the group consisting of, for example, sterile milk or whey or synthetic, commercially prepared media containing casein, casein hydrolysates or peptone, such as e.g. Man-Rogosa-Sharpe, AOAC, Trypticase Soy Broth, Bacto Proteose Peptone, Reinforced Clostridial Broth, Tryptone, Peptone or mixtures thereof. In the method, the cultivation of the probiotic culture may be carried out in a culture medium or in a mixture of the culture media or stepwise in at least two different culture media or mixtures thereof. Cultivation may be static or with continuous stirring or in a combined manner, i. Static at times and temporarily with stirring.

The thickening leading to the increase in concentration can be carried out, for example, as centrifugation or ultrafiltration and, for example, a fluid or freeze drier can be used for dehydration.

In an advantageous embodiment of the method, the thickened preparation is dried with the addition of another carrier, the latter being selected from the group comprising maltodextrin, inulin or mixtures thereof, thereby facilitating dehydration, increasing the yield and subsequently processing the material can be simplified.

After dehydration tablets can be pressed from the preparation. The preparation in the form of a powder or granules can also be filled into capsules, bags or containers with drinks.

The preparation according to the invention can be used, for example, as a dietary supplement, special nutrient, foodstuff, drink, remedy, health product, cosmetic and hygienic product, animal feed product or a probiotics-containing supplement for human and veterinary use.

Exemplary embodiments of the invention

example 1

The probiotic culture Lactobacillus acidophillus is propagated on a solid MRS medium for 24 hours at 37 ° C. Subsequently, at least one colony is removed with a microbiological loop, which is then transferred to an Erlenmayer flask of the total volume of 300 ml, the flask containing 100 ml of the pre-sterilized MRS medium. The cultivation proceeds either stationary or with gentle shaking for 16 to 24 hours at 37 ° C. This is followed by transferring the contents of the Erlenmayer flask to a fermenter containing 2 liters of a pre-sterilized culture medium, such as sterile milk or whey, or synthetic, commercially prepared media containing casein or casein hydrolyzates, e.g. Man-Rogosa-Sharpe, AOAC, Trypticase Soy Broth, Bacto Proteose Peptone, Reinforced Clostridial Broth, Tryptone, Peptone or the like. The medium is pre-sterilized along with the corn starch carrier (20% by weight). The cultivation proceeds for 20 hours at 37 ° C and 90 rpm. This is followed by 6 to 8 hours of static culture without shaking at 37 ° C. During the process, the formation of the bacterial coverage of the carriers is continuously checked. Once at least 5% of the carriers are covered with bacteria, the cultivation can be stopped. After completion of the culture, the culture is spun in a flow-through centrifuge or processed in an ultrafiltration unit and then it is lyophilized with the addition of maltodextrin in the amount of 40 wt.%.

Example 2

The probiotic culture Bifidobacterium is fixed on a. Casein hydrolyzate containing agar medium for 24 to 48 hours at 37 ° C propagated. Subsequently, at least one colony is removed with a microbiological loop, which is then transferred to an Erlenmayer flask of 300 ml total volume, the flask containing 50 to 100 ml of pre-sterilized MRS medium (Man-Rogosa-Sharpe). The cultivation proceeds either stationary or with gentle shaking for 8 to 16 hours at 37 ° C. Following is the transfer of the contents of the Erlenmayer flask to a 10 liter culture flask containing 6 liters of pre-sterilized MRS culture medium. The probiotic culture is obtained by static culture for 8 to 16 hours runs. Then the preparation of the medium in the amount of 170 l follows (15 kg dry whey are dissolved in purified water in the amount of 170 l and then the solution is sterilized with the addition of 15 kg of microcrystalline cellulose carrier). In the thus prepared medium, the content of the 10-liter culture bottle is transferred. The cultivation proceeds for 20 hours at 37 ° C and at the speed of the agitator of 90 rpm. Static culture then proceeds at 37 ° C for 20 hours with minimal air supply (10 liters of atmospheric air per hour). During the process, the formation of the bacterial coverage of the carriers is continuously checked. Once at least 5% of the carriers are covered with bacteria, the cultivation can be stopped. After completion of the culture, the culture is spun in a flow-through centrifuge and then dried in a fluid dryer with the addition of maltodextrin or inulin in the ratio of 2: 8.

Example 3

The probiotic culture Lactobacillus rhamnosus is propagated on a solid MRS medium for 24 to 48 hours at 37 ° C. Subsequently, a microbiological loop is used to remove at least one colony, which is then transferred to an Erlenmayer flask of a total volume of 300 ml, the flask containing 50 to 100 ml of the pre-sterilized trypticase soy broth medium, which has a pH of 6 is set. The cultivation proceeds either stationary or with gentle shaking for 12 to 16 hours at 37 ° C. Following is the transfer of the contents of the Erlenmayer flask to a 10 liter culture flask containing 6 liters of pre-sterilized culture medium. The probiotic culture is obtained by static culture, which lasts 8 to 16 hours. Then follows the preparation of the culture medium (7 kg of the medium Reinforced Clostridial Broth are dissolved in purified water in the amount of 190 l and then the solution with the addition of 15 kg of the prepared in the ratio of 1: 1 mixture of microcrystalline cellulose and the corn starch existing carrier sterilized). In the thus prepared medium, the content of the 10-liter culture bottle is transferred. The cultivation proceeds for 20 hours at 37 ° C and at the speed of the agitator of 120 rpm. Static culture then proceeds at 37 ° C for 4 to 16 hours with minimal air supply (10 liters of atmospheric air per hour). During the process, the formation of the bacterial coverage of the carriers is continuously checked. Once at least 5% of the carriers are covered with bacteria, the cultivation can be stopped. After completion of the culture, the culture is spun in a flow-through centrifuge, and then it is dried in a fluid dryer with 2:10 maltodextrin or inulin added.

Example 4

The probiotic culture Streptococcus thermophilus is propagated on a solid MRS medium for 24 to 48 hours at 39 ° C. Subsequently, a microbiological loop is used to remove at least one colony, which is then transferred to an Erlenmayer flask of a total volume of 300 ml, the flask containing 50 to 100 ml of the presterilized Bacto Proteose Peptone medium. The cultivation proceeds either stationary or with gentle shaking for 12 to 16 hours at 39 ° C. Following is the inoculation, which consists in placing the contents of the Erlenmayer flask in a 10 liter culture flask containing 6 liters of pre-sterilized culture medium. The probiotic culture is obtained by static culture, which lasts 8 to 16 hours. Then follows the preparation of the culture medium (7 kg of the powdered medium Bacto Proteose peptone are dissolved in purified water in the amount of 190 l and then the solution with the addition of 25 kg of the prepared in the ratio of 1: 1 mixture of microcrystalline cellulose and the corn starch existing carrier is sterilized). In the thus prepared medium, the content of the 10-liter culture bottle is transferred. The cultivation proceeds for 12 to 16 hours at 39 ° C and at the speed of the agitator of 120 rpm. Static culture then proceeds at 37 ° C for 4 to 16 hours with minimal air supply (20 liters of atmospheric air per hour). During the process, the formation of the bacterial coverage of the carriers is continuously checked. Once at least 5% of the carriers are covered with bacteria, the cultivation can be stopped. After completion of the culture, the culture is spun in a flow-through centrifuge, and then it is dried in a fluid dryer with 2:10 maltodextrin or inulin added.

Example 5

A mixture of the probiotic cultures Lactobacillus rhamnosus, Bifidobacterium breve, Lactobacillus casei, Lactobacillus acidophilus and Bifidobacterium longum is obtained by the fermentation-proceeding culture on a liquid medium prepared from the dry whey dissolved in the demineralized water. The ampoules containing the lyophilisates of the individual cultures are rehydrated with 1 ml of physiological saline and the contents thus dissolved are transferred to an Erlenmayer flask of a total volume of 300 ml, the Contains 200 ml of a pre-sterilized medium prepared by dissolving 18 g of dry whey in demineralised water (200 ml). The cultivation proceeds either stationary or with gentle shaking for 12 to 16 hours at 37 ° C. Following is the transfer of the contents of the Erlenmayer flask to a 10 liter culture flask containing 6 liters of pre-sterilized culture medium prepared by dissolving 540 grams of dry whey in demineralized water (6 liters). The probiotic culture is obtained by static culture, which lasts 8 to 16 hours. Then follows the preparation of the culture medium (9 kg dry whey dissolved in demineralized water in the amount of 100 liters and then sterilized with the addition of a carrier in the amount of 25 kg, using as the carrier microcrystalline cellulose or starch or inulin or glycogen or dextran can be used, in separate form or in a mixture). After circulating the inoculum in the amount of 6 liters in the medium in the amount of 100 liters, the cultivation proceeds for 12 to 16 hours at 37 ° C and at the speed of the agitator of 120 rpm. Subsequently, the static cultivation proceeds at 34 ° C for 4 to 16 hours. During the process, the formation of the bacterial coverage of the carriers is continuously checked. Once at least 5% of the carriers are covered with bacteria, the cultivation can be stopped. After completion of the culture, the culture is spun in a flow-through centrifuge or thickened in an ultrafiltration unit and then it is dried in a fluid dryer with the addition of maltodextrin or inulin in a ratio of 2: 3.

Example 6

The probiotic culture Lactobacillus acidophillus is propagated on a solid MRS medium for 24 to 48 hours at 37 ° C. Subsequently, at least one colony is removed with a microbiological loop, which is then transferred to an Erlenmayer flask of a total volume of 300 ml, the flask containing 50 to 100 ml of the presterilized MRS medium, which is adjusted to a pH of 6 is. The cultivation proceeds either stationary or with gentle shaking for 12 to 16 hours at 37 ° C. Following is the transfer of the contents of the Erlenmayer flask to a 10 liter culture flask containing 6 liters of the pre-sterilized MRS culture medium. The probiotic culture is obtained by static culture, which lasts 8 to 16 hours. This is followed by the preparation of 170 liters of the MRS culture medium, which was presterilized with the addition of the carrier in the amount of 15 kg. The support is in the form of a mixture of microcrystalline cellulose and corn starch prepared in the ratio of 1: 1. The medium is processed in a fermenter. The content of the 10 liter culture bottle is then transferred to the pre-sterilized medium. The cultivation proceeds for 12 to 16 hours at 37 ° C and at the speed of the agitator of 120 rpm. Static culture then proceeds at 37 ° C for 2 to 16 hours with minimal air flow (10 liters of atmospheric air per hour). During the process, the formation of the bacterial coverage of the carriers is continuously checked. Once at least 5% of the carriers are covered with bacteria, the cultivation can be stopped. After completion of the culture, the culture is spun in a flow-through centrifuge, and then it is dried in a fluid dryer with the addition of maltodextrin or inulin in a ratio of 2:10.

QUOTES INCLUDE IN THE DESCRIPTION

This list of the documents listed by the applicant has been generated automatically and is included solely for the better information of the reader. The list is not part of the German patent or utility model application. The DPMA assumes no liability for any errors or omissions.

Cited non-patent literature

• Zobel et al., 1935 [0002]
• Costerton et al., 1985 [0002]
• Costerton JW 1999, Stoodley et al., 2002 [0005]
• O'Toole et al., 2000; Donlan et Costerton 2002 [0006]
• Rickard et al., 2003 [0006]
• Costerton et al., 1995 [0007]
• Costerton et al., 1999 [0007]
• Stoodley et al., 2002 [0007]
• Costerton et al., 1995; O'Tooley et al., 2000 [0009]
• Hausner et al., 1999 [0009]

Claims (9)

A preparation for use in the food industry, pharmaceutical industry, human or veterinary medicine or cosmetics, characterized in that it comprises a probiotic culture in the form of a single or multiple probiotic in the amount of 1 x 10 ³ to 1 x 10 ¹⁴ CFU per 1 g of the preparation, and a carrier in the amount of 0.001 to 99 wt.% Contains, wherein the probiotic culture is at least partially in the form of a biofilm adhering to the carrier. Preparation according to claim 1, characterized in that the probiotic culture is selected from the group consisting of the genera Lactobacillus, Bifidobacterium, Streptococcus, Sacharomyces or other bacteria beneficial to human or animal health or constituting a natural constituent of human or animal microflora or yeasts, optionally their mixture. A preparation according to any one of claims 1 to 2, characterized in that the carrier is selected from the group consisting of oligosaccharides and polysaccharides such as microcrystalline cellulose (MCC), pectin, inulin, dextrin, maltodextrin, glycogen, corn or potato starch and other starches , Proteins of microbial, plant or animal origin, crystals of various mineral substances, HMC, nanotextiles, FOS, GOS and other physiologically acceptable carriers or mixtures thereof. Preparation according to one of claims 1 to 3, characterized in that the carrier is contained in the preparation in the minimum amount of 1 wt.%. A preparation according to any one of claims 1 to 4, characterized in that the probiotic culture is contained in the preparation in the amount of 1 x 10 ⁷ CFU / g. A preparation according to any one of claims 1 to 5, characterized in that 0.1 to 100% by weight of the probiotic culture is in the form of a biofilm. Preparation according to one of claims 1 to 6, characterized in that it further contains vitamins, minerals, vegetable extracts and other physiologically beneficial substances in the maximum amount of 99 wt.%. Preparation according to one of claims 1 to 7, characterized in that it is in the form of a tablet, a capsule, a powder or a granule. Food product, pharmaceutical product or veterinary product containing the preparation according to any one of claims 1 to 8.