CN109055348A - Utilize the method for lactobacillus-fermented agricultural and sideline product preparation γ-aminobutyric acid - Google Patents
Utilize the method for lactobacillus-fermented agricultural and sideline product preparation γ-aminobutyric acid Download PDFInfo
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- CN109055348A CN109055348A CN201811046706.6A CN201811046706A CN109055348A CN 109055348 A CN109055348 A CN 109055348A CN 201811046706 A CN201811046706 A CN 201811046706A CN 109055348 A CN109055348 A CN 109055348A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
- C12N11/12—Cellulose or derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/005—Amino acids other than alpha- or beta amino acids, e.g. gamma amino acids
Abstract
Using the method for lactobacillus-fermented agricultural and sideline product preparation γ-aminobutyric acid, belong to agricultural and sideline product biological processing technical field.Its step includes: S1, the outstanding bacterium solution of making lactic acid bacterium；S2, lactic acid bacteria are fixed；S3, the obtained fixing lactic acid bacteria of the step S2 is inoculated into the fluid nutrient medium containing agricultural and sideline product, is sufficiently stirred, carries out liquid fermentation.Lactic acid bacteria is fixed using sericin, cellulose and oxidized dextran containing more aldehyde radicals in the present invention, has the characteristics that biocompatibility is high, safe and non-toxic, stability is good, promote lactic acid bacteria proliferation；Using discarded agricultural and sideline product as fermented and cultured substrate, it can be achieved that resource circulation utilization, not only containing the nutriment of agricultural and sideline product in obtained fermentation liquid, but also contains a large amount of γ-aminobutyric acids, have very high nutritive value.
Description
Technical field
The invention belongs to agricultural and sideline product biological processing technical fields, and in particular to a kind of to utilize fixing lactic acid bacteria fermentation agriculture
The method of byproduct preparation γ-aminobutyric acid.
Background technique
γ-aminobutyric acid (GABA) also known as amino acid injection-800, are a kind of white crystalline powders, are distributed widely in animal and plant body
Interior non-protein amino acid is the main inhibitory neurotransmitter of central nervous system.GABA has important physiological function:
(1) improve memory, enhance memory function；(2) to the facilitation effect of growth hormone secretion, growth hormone has promotion skeletal muscle
The effects of enhancing of meat improves immunity, reduces fat, improve body function；(3) analgesia, calm and improvement sleep effect；
(4) there are alleviation and therapeutic effect to hypertension.
γ-aminobutyric acid preparation method mainly has chemical synthesis and two kinds of biological synthesis process, and chemical synthesis process is main
It is (180 DEG C) reactions under intense conditions with O-phthalic imide potassium and γ-neoprene cyanogen, product and concentrated sulfuric acid back hydrolysis,
It is obtained using crystallization and purification.Although chemical synthesis GABA is swift in response, but violent, retrial is by force with reaction condition,
The disadvantages of energy consumption is high, at high cost, side reaction is more and environmental pollution is serious.In comparison biological synthesis process is safer, cost
Also low.
Biological synthesis process is mainly the glutamate decarboxylase (Glutamate decarboxylase, GAD) for utilizing organism
Decarboxylic reaction occurs for the α-carboxyl for being catalyzed Pidolidone or Pidolidone salt, to generate GABA.Its product have it is at low cost, contain
The advantages of amount is high and can be used safely in food, but the usually more difficult acquisition of efficient microorganism fungus kind.Existing document passes through saliva chain
Coccus thermophilous subspecies, Pediococcus pentosaceus, enterococcus faecium, short and small lactobacillus, Bacillus coli cells conversion method prepare the report of GABA.
But since free cell is not easily recycled reuse, there is still a need for the continuous cells for repeating to cultivate high GAD vigor to be used to give birth to
It produces, and by immobilization technology, the cells are fixed or is embedded on solid-state carrier, that is, is prepared into immobilized cell, then can overcome trip
Cellifugal disadvantage, and can realize continuous production.
Agricultural and sideline product is rich in GABA, develops and uses the agricultural and sideline product, its added value, especially rice, corn can be improved
With large agricultural and sideline product such as beans, using lactic acid bacteria can ferment this kind of agricultural and sideline product advantage carry out GABA preparation method, into
One step enriches health-care efficacy of the lactic acid bacteria as beneficial bacterium, additionally it is possible to be enriched with GABA content by biotechnology, improve agricultural and sideline
The exploitation new way of product, is conducive to the comprehensive utilization of agricultural and sideline product.
Summary of the invention
In view of the problems of the existing technology, lactobacillus-fermented agricultural and sideline product is utilized the purpose of the present invention is to provide a kind of
The method for preparing γ-aminobutyric acid, this method is easy to operate, and raw material is easily obtained, and is suitble to industrialized production, and obtained hair
γ-aminobutyric acid is rich in zymotic fluid.
The technical solution adopted by the present invention to solve the technical problems is:
Using the method for lactobacillus-fermented agricultural and sideline product preparation γ-aminobutyric acid, include the following steps:
S1, making lactic acid bacterium hang bacterium solution: by lactobacillus inoculum in liquid enriched medium, logarithm is obtained after shaking table culture
The thallus in growth period after centrifugation, by thallus with brine 2~3 times, is collected by centrifugation, after normal saline dilution again
Obtain lactic acid bacteria suspension；
S2, lactic acid bacteria are fixed:
1) sericin is swollen sufficiently with acetic acid, be added in the lactic acid bacteria suspension that the step S1 is prepared, fill
Divide and is mixed to get liquid A；
2) liquid A that the step 1) obtains is sufficiently mixed with cellulose, obtains liquid B；
3) oxidized dextran containing more aldehyde radicals is added in the liquid B obtained to the step 2), 20~40 DEG C with 100~
The speed oscillation of 500r/min is crosslinked 4~6h, obtains immobilization bacterium solution；
4) the immobilization bacterium solution that the step 3) obtains sufficiently is washed with the 0.2mol/L phosphate buffer of pH=6.8
Afterwards, it is centrifuged, obtains fixing lactic acid bacteria.
S3, fermentation agricultural and sideline product prepare γ-aminobutyric acid: the fixing lactic acid bacteria that the step 4) obtains being inoculated into and is contained
It in the fluid nutrient medium of agricultural and sideline product, is sufficiently stirred, carries out liquid fermentation.
Oxidized dextran containing more aldehyde radicals can be made by laxative remedy: weigh glucan (preferably T-40) 10.0g, sodium metaperiodate
13.5g is dissolved in respectively in 200ml and 150mlpH4.4 phosphate buffer, and sodium periodate solution is added in dextran solution,
1500r/min is protected from light stirring 4.5h at room temperature, and glycerine 4.5ml is added immediately, and room temperature 1500r/min continues to stir 15min, will
Reaction mixture is placed in the bag filter for the molecular weight 3500 that shuts off, and using distilled water as 4 DEG C of dialysis 48h of medium, dialyzate freezing is dry
It is dry, obtain oxidized dextran.
Sericin is the byproduct of mulberry silk industry, and as a kind of good biocompatibility, cell adherence is good, nontoxic, nothing
Pollution, non-stimulated, biodegradable native protein, are widely used in biologic medical field and cosmetic field now,
It is rich in amino, carboxyl isoreactivity group.Lactic acid bacteria is generally negatively charged, and sericin is positively charged in acid condition, leads to
Electrostatic attraction lactic acid bacteria is crossed to be adsorbed on sericin.Cellulose belongs to a kind of polysaccharide of safety of workers, not still plant
The most important component part of body, and be also the representative of the outer macromolecular substances of microbial cell.The addition of cellulose helps improve
The swelling behavior of material can be such that material further hardens, the mechanical performance of reinforcing material.Cellulose has ultra-fine three-dimensional netted more
Pore structure, can by lactic acid bacteria, by sericin adsorb lactic acid bacteria uniform adsorption and be firmly integrated to its surface and inside, this
Outside, cellulose has better mechanical performance, excellent biodegradability and thermal stability.Glucan itself possess it is nontoxic,
Good water solubility, cheap feature and a kind of raw material for bio-medical material of classics.Oxidation Portugal containing more aldehyde radicals
Glycan is a kind of macromolecule glucose polymer that natural glucan obtains after sodium periodate oxidation, is used as crosslinking agent,
Stable crosslinking can be provided, and biocompatibility is fabulous, calcification and the toxic reaction of cell will not be caused.Oxidized dextran contains
Multiple aldehyde radicals carry out hemiacetal and schiff bases with the amino in the hydroxyl and sericin of high reaction activity in cellulose respectively
Sericin grafting is led on cellulose macromolecule, forms the fixing lactic acid bacteria with tridimensional network by reaction.Due to tool
The surface layer for having the macromolecular fibre " filament " formed by cross-linking reaction to constitute and reticulate body, " filament " diameter is small, and quantity is more, so
The specific surface area of microbial material is huge, to improve in each biological processing unit of the microbial material in unit volume
The quantity of microorganism.
Sericin is a kind of natural macromolecular albumen, is mainly made of 18 kinds of amino acid, and content is preferably serine,
Secondary is aspartic acid and glutamic acid, these three amino acid contents account for 60% or more of sericin total amino acid content.Aoxidize Portugal
Glycan can also degrade, and catabolite is amino acid and polysaccharide.Therefore, sericin and oxidized dextran may be used also in the present invention
Certain fermentation substrate is provided for lactic acid bacteria, is conducive to the proliferation of bacterium.
Preferably, the viable count in the step S1 in lactic acid bacteria suspension is 1 × 107~1 × 109CFU/ml。
Preferably, cellulose is cellulose acetate or carboxymethyl cellulose or DEAE cellulose in the step 2).
Preferably, lactic acid bacteria suspension, sericin, cellulose, the oxidation Portugal containing more aldehyde radicals in the step S2
The mass ratio of glycan is 10~20:0.8~1.6:0.5~2:0.2~0.8.
More preferably, lactic acid bacteria suspension, sericin, cellulose, the oxidation Portugal containing more aldehyde radicals in the step S2
The mass ratio of glycan is 15:1.2:2:0.4.
Preferably, thering is liquid A and cellulose to be sufficiently mixed mode in the step 2) are as follows: cellulose first uses deionization
Water impregnate 30-40h obtain swollen cellulose, then by swollen cellulose and solution A at 37 DEG C constant temperature water bath stirring 1-2h or
On cellulose by solution A Direct spraying.
Preferably, the inoculum concentration of fixing lactic acid bacteria is 0.5-5%, the liquid fermentation condition in the step S3
Are as follows: fermentation temperature is 30-38 DEG C, fermentation time 20-30h, revolving speed 100-300r/min.
More preferably, the inoculum concentration of S3 fixing lactic acid bacteria is 1% in the step, the liquid fermentation condition are as follows:
35 DEG C of fermentation temperature, fermentation time is fermentation shake flask revolving speed 150r/min for 24 hours.
Preferably, the component of the fluid nutrient medium containing agricultural and sideline product includes glucose 20-30g/ in the step S3
L, brown rice 40-50g/L, corn flour 15-20g/L, soy meal 5-10g/L, sodium glutamate 5-10g/L, VB 15-20mg/L, VH
5-10mg/L。
More preferably, in the step S3 component of the fluid nutrient medium containing agricultural and sideline product include glucose 30g/L, it is rough
Rice 40g/L, corn flour 20g/L, soy meal 10g/L, sodium glutamate 8g/L, VB0.02g/L and VH0.01g/L.
The present invention have it is following the utility model has the advantages that
1) lactic acid bacteria is adsorbed on sericin by the present invention first, then adsorbs with cellulose, with the aldehyde of oxidized dextran
Base connects the cellulose of amino-containing sericin and hydroxyl as intermediate, and consolidating with tridimensional network is prepared
Surely change lactic acid bacteria, short with the transformation period, it is convenient that conversion medium liquid is prepared, and interfering substance is few in reaction system, it is not easy microbiological contamination,
The advantages that product is more single, and cell density is high, and thallus can reuse, and product can be easily separated, can reach and improve efficiency, and reduce
The purpose of cost；
2) lactic acid bacteria is fixed using sericin, cellulose and oxidized dextran containing more aldehyde radicals in the present invention, had
Biocompatibility is high, safe and non-toxic, stability is good, promotes the features such as lactic acid bacteria proliferation；
3) process for fixation of lactic acid bacteria provided by the invention is simple, and condition requires mild, it is easy to accomplish, it is obtained solid
Surely changing lactic acid has many advantages, such as that vigor is high, load capacity is big, the catalysis reaction service life is long；
4) the achievable resource circulation utilization of the present invention, not only containing the nutriment of agricultural and sideline product in obtained fermentation liquid, but also
Containing a large amount of γ-aminobutyric acids, have very high nutritive value.
Specific embodiment
The present invention is further illustrated below in conjunction with specific embodiment.
Embodiment 1
Using the method for lactobacillus-fermented agricultural and sideline product preparation γ-aminobutyric acid, include the following steps:
S1, making lactic acid bacterium suspension:
The thallus that logarithmic growth phase is obtained by lactobacillus inoculum in liquid enriched medium, after shaking table culture, after centrifugation,
It by thallus with brine 3 times, is collected by centrifugation again, with obtaining lactic acid bacteria suspension, the cream after normal saline dilution
Viable count in sour bacterium suspension is 108CFU/ml。
The immobilization of S2, lactic acid bacteria:
15ml lactic acid bacteria suspension is taken, 1.2g sericin is added, is sufficiently mixed to obtain liquid A；Liquid A is sprayed to
Mixing liquid B is obtained on 1.8g carboxymethyl cellulose；0.4g is added in liquid B containing the oxidized dextran of more aldehyde radicals, 37
6h is crosslinked with the speed oscillation of 300r/min at DEG C, obtains immobilization bacterium solution；The 0.2mol/L phosphorus of immobilization bacterium solution pH=6.8
Phthalate buffer sufficiently wash after, it is centrifugation, dry, obtain fixing lactic acid bacteria.
S3, fermentation agricultural and sideline product preparation preparation γ-aminobutyric acid
It prepares the fluid nutrient medium containing agricultural and sideline product: being put into 500ml water in 1000ml beaker, be separately added into glucose
VB20mg and VH 10mg, stirring is added in 30g, brown rice 50g, corn flour 20g, soy meal 10g, sodium glutamate 8g after mixing
Uniformly, it is settled to 1000ml, is added culture medium 150ml in 300ml triangular flask, wraps up bottleneck, in 115 DEG C of sterilizing 20min,
It is spare that cooling is taken out after the completion of sterilizing；
Fixing lactic acid bacteria is inoculated in the above-mentioned triangular flask for filling culture medium according to 1% ratio, controls fermentation temperature
It 35 DEG C, fermentation shake flask revolving speed 150r/min, cultivates 24 hours, carries out converting agricultural byproduct into gamma-aminobutyric acid fermented and cultured；
It will test the γ-aminobutyric acid in fermentation liquid with high-content, GABA content is about 350mg/100ml.
Embodiment 2
Using the method for lactobacillus-fermented agricultural and sideline product preparation γ-aminobutyric acid, include the following steps:
S1, making lactic acid bacterium suspension:
The thallus that logarithmic growth phase is obtained by lactobacillus inoculum in liquid enriched medium, after shaking table culture, after centrifugation,
It by thallus with brine 3 times, is collected by centrifugation again, with obtaining lactic acid bacteria suspension, the cream after normal saline dilution
Viable count in sour bacterium suspension is 108CFU/ml。
The immobilization of S2, lactic acid bacteria:
10ml lactic acid bacteria suspension is taken, 1.0g sericin is added, is sufficiently mixed to obtain liquid A；Cellulose acetate is used
Deionized water impregnates 35h and obtains swollen cellulose, and then by swollen cellulose and solution A, constant temperature water bath stirs 1- at 37 DEG C
2h obtains mixing liquid B；0.3g is added in liquid B containing the oxidized dextran of more aldehyde radicals, with 300r/min's at 35 DEG C
Speed oscillation is crosslinked 6h, obtains immobilization bacterium solution；Immobilization bacterium solution is sufficiently washed with the 0.2mol/L phosphate buffer of pH=6.8
It after washing, is centrifuged, is dry, obtaining fixing lactic acid bacteria.
S3, fermentation agricultural and sideline product preparation preparation γ-aminobutyric acid
It prepares the fluid nutrient medium containing agricultural and sideline product: being put into 500ml water in 1000ml beaker, be separately added into glucose
VB15mg and VH 10mg, stirring is added in 20g, brown rice 40g, corn flour 15g, soy meal 7g, sodium glutamate 5g after mixing
Uniformly, it is settled to 1000ml, is added culture medium 150ml in 300ml triangular flask, wraps up bottleneck, in 115 DEG C of sterilizing 20min,
It is spare that cooling is taken out after the completion of sterilizing；
Fixing lactic acid bacteria is inoculated in the above-mentioned triangular flask for filling culture medium according to 1% ratio, controls fermentation temperature
It 30 DEG C, fermentation shake flask revolving speed 300r/min, cultivates 24 hours, carries out converting agricultural byproduct into gamma-aminobutyric acid fermented and cultured；
It will test the γ-aminobutyric acid in fermentation liquid with high-content, GABA content is about 320mg/100ml.
Embodiment 3
Using the method for lactobacillus-fermented agricultural and sideline product preparation γ-aminobutyric acid, include the following steps:
S1, making lactic acid bacterium suspension:
The thallus that logarithmic growth phase is obtained by lactobacillus inoculum in liquid enriched medium, after shaking table culture, after centrifugation,
It by thallus with brine 3 times, is collected by centrifugation again, with obtaining lactic acid bacteria suspension, the cream after normal saline dilution
Viable count in sour bacterium suspension is 109CFU/ml。
The immobilization of S2, lactic acid bacteria:
12ml lactic acid bacteria suspension is taken, 1.6g sericin is added, is sufficiently mixed to obtain liquid A；DEAE cellulose is used
Deionized water impregnates 30h and obtains swollen cellulose, and then by swollen cellulose and solution A, constant temperature water bath stirs 2h at 37 DEG C,
Obtain mixing liquid B；0.3g is added in liquid B containing the oxidized dextran of more aldehyde radicals, is turned at 35 DEG C with 300r/min
Speed oscillation crosslinking 6h, obtains immobilization bacterium solution；Immobilization bacterium solution is sufficiently washed with the 0.2mol/L phosphate buffer of pH=6.8
Afterwards, it is centrifuged, is dry, obtaining fixing lactic acid bacteria.
S3, fermentation agricultural and sideline product preparation preparation γ-aminobutyric acid
It prepares the fluid nutrient medium containing agricultural and sideline product: being put into 500ml water in 1000ml beaker, be separately added into glucose
VB15mg and VH 10mg, stirring is added in 20g, brown rice 40g, corn flour 15g, soy meal 7g, sodium glutamate 5g after mixing
Uniformly, it is settled to 1000ml, is added culture medium 150ml in 300ml triangular flask, wraps up bottleneck, in 115 DEG C of sterilizing 20min,
It is spare that cooling is taken out after the completion of sterilizing；
Fixing lactic acid bacteria is inoculated in the above-mentioned triangular flask for filling culture medium according to 0.5% ratio, control fermentation temperature
38 DEG C, fermentation shake flask revolving speed 100r/min of degree is cultivated 30 hours, and converting agricultural byproduct into gamma-aminobutyric acid fermentation training is carried out
It supports；It will test the γ-aminobutyric acid in fermentation liquid with high-content, GABA content is about 370mg/100ml.
The preferred embodiments of the invention are only listed above, and protection scope of the present invention is not restricted to this, this field
Made any change is each fallen in the scope of the present invention technical staff within the scope of the invention as claimed.
Claims (10)
1. utilizing the method for lactobacillus-fermented agricultural and sideline product preparation γ-aminobutyric acid, which is characterized in that the preparation method includes
Following steps:
S1, making lactic acid bacterium hang bacterium solution: by lactobacillus inoculum in liquid enriched medium, logarithmic growth is obtained after shaking table culture
The thallus of phase after centrifugation, by thallus with brine 2~3 times, is collected by centrifugation again, with obtaining after normal saline dilution
Lactic acid bacteria suspension；
S2, lactic acid bacteria are fixed:
1) sericin is swollen sufficiently with acetic acid, be added in the lactic acid bacteria suspension that the step S1 is prepared, it is sufficiently mixed
Conjunction obtains liquid A；
2) liquid A obtained to the step 1) is added cellulose and is sufficiently mixed, and obtains liquid B；
3) oxidized dextran containing more aldehyde radicals is added in the liquid B obtained to the step 2), 20~40 DEG C with 100~
The speed oscillation of 500r/min is crosslinked 4~6h, obtains immobilization bacterium solution；
After 4) sufficiently washing the 0.2mol/L phosphate buffer for the immobilization bacterium solution pH=6.8 that the step 3) obtains,
Centrifugation, obtains fixing lactic acid bacteria；
S3, fermentation agricultural and sideline product prepare γ-aminobutyric acid: the fixing lactic acid bacteria that the step 4) obtains is inoculated into containing agricultural and sideline
It in the fluid nutrient medium of product, is sufficiently stirred, carries out liquid fermentation.
2. the method according to claim 1 using lactobacillus-fermented agricultural and sideline product preparation γ-aminobutyric acid, feature exist
In the viable count in the step S1 in lactic acid bacteria suspension is 1 × 107~1 × 109CFU/ml。
3. the method according to claim 1 using lactobacillus-fermented agricultural and sideline product preparation γ-aminobutyric acid, feature exist
In the cellulose is cellulose acetate or carboxymethyl cellulose or DEAE cellulose.
4. the method according to claim 1 using lactobacillus-fermented agricultural and sideline product preparation γ-aminobutyric acid, feature exist
In the mass ratio of, lactic acid bacteria suspension in the step S2, sericin, cellulose, oxidized dextran containing more aldehyde radicals be 10
~20:0.8~1.6:0.5~2:0.2~0.8.
5. the method according to claim 4 using lactobacillus-fermented agricultural and sideline product preparation γ-aminobutyric acid, feature exist
In the mass ratio of, lactic acid bacteria suspension in the step S2, sericin, cellulose, oxidized dextran containing more aldehyde radicals be 15:
1.2:2:0.4.
6. the method according to claim 1 using lactobacillus-fermented agricultural and sideline product preparation γ-aminobutyric acid, feature exist
In liquid A and cellulose are sufficiently mixed mode in the step 2) are as follows: cellulose is first obtained with deionized water immersion 30-40h molten
Swollen cellulose, then by swollen cellulose and solution A, constant temperature water bath stirs 1-2h or by solution A Direct spraying in fibre at 37 DEG C
On dimension element.
7. the method according to claim 1 using lactobacillus-fermented agricultural and sideline product preparation γ-aminobutyric acid, feature exist
In the inoculum concentration of fixing lactic acid bacteria is 0.5-5%, the liquid fermentation condition are as follows: fermentation temperature 30- in the step S3
38 DEG C, fermentation time 20-30h, revolving speed 100-300r/min.
8. the method according to claim 7 using lactobacillus-fermented agricultural and sideline product preparation γ-aminobutyric acid, feature exist
In the inoculum concentration of fixing lactic acid bacteria is 1% in the step S3, the liquid fermentation condition are as follows: 35 DEG C of fermentation temperature, fermentation
Time is fermentation shake flask revolving speed 150r/min for 24 hours.
9. the method according to claim 1 using lactobacillus-fermented agricultural and sideline product preparation γ-aminobutyric acid, feature exist
In the component of the fluid nutrient medium containing agricultural and sideline product includes glucose 20-30g/L, brown rice 40-50g/L, jade in the step S3
Rice flour 15-20g/L, soy meal 5-10g/L, sodium glutamate 5-10g/L, VB 15-20mg/L, VH 5-10mg/L.
10. the method according to claim 9 using lactobacillus-fermented agricultural and sideline product preparation γ-aminobutyric acid, feature
It is, the component of the fluid nutrient medium containing agricultural and sideline product includes glucose 30g/L, brown rice 40g/L, corn flour 20g/L, soy meal
10g/L, sodium glutamate 8g/L, VB0.02g/L and VH0.01g/L.
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| CN111607539A (en) * | 2020-06-01 | 2020-09-01 | 鲁东大学 | Chinese cabbage extract culture medium and method for producing gamma-aminobutyric acid through fermentation |
| CN117431275A (en) * | 2023-12-21 | 2024-01-23 | 广州巴宝莉化妆品有限公司 | Preparation method and application of lactobacillus fermentation broth |
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| CN101838672A (en) * | 2010-05-10 | 2010-09-22 | 江南大学 | Method for producing gamma-amino butyric acid by using immobilized lactobacillus plantarum |
| CN105985529A (en) * | 2015-03-05 | 2016-10-05 | 华中科技大学同济医学院附属协和医院 | Sericin-alginate compounded hydrogel and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111607539A (en) * | 2020-06-01 | 2020-09-01 | 鲁东大学 | Chinese cabbage extract culture medium and method for producing gamma-aminobutyric acid through fermentation |
| CN117431275A (en) * | 2023-12-21 | 2024-01-23 | 广州巴宝莉化妆品有限公司 | Preparation method and application of lactobacillus fermentation broth |
| CN117431275B (en) * | 2023-12-21 | 2024-03-29 | 广州巴宝莉化妆品有限公司 | Preparation method and application of lactobacillus fermentation broth |
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