CN106148451B - Biological cellulose gel culture medium and the biological cellulose gel product prepared with it - Google Patents
Biological cellulose gel culture medium and the biological cellulose gel product prepared with it Download PDFInfo
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- CN106148451B CN106148451B CN201510138896.4A CN201510138896A CN106148451B CN 106148451 B CN106148451 B CN 106148451B CN 201510138896 A CN201510138896 A CN 201510138896A CN 106148451 B CN106148451 B CN 106148451B
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Abstract
The present invention relates to a kind of biological cellulose gel culture mediums, wherein containing spirulina medium.The spirulina medium is the fluid nutrient medium by being improved with viscosity, the culture preparation in spirulina 3-10 days under conditions of illumination is sufficient, temperature is 20-35 DEG C.The present invention improves the dissolved oxygen amount in biological cellulose gel culture medium by the photosynthesis of spirulina, when static culture, at the time of capable of postponing biology cellulose producing strains anoxic, improves the yield and quality of biological cellulose gel.Spirulina is wrapped up with biological cellulose gel, the oxidation of spirulina can also be effectively avoided, avoid the loss and discoloration of nutritional ingredient, and further promote product commercial value using the various health care functions of spirulina, and obtain good green appearance.
Description
Technical field
The present invention relates to the preparation of biology cellulose product, in particular to a kind of biological cellulose gel culture medium, with this
Application of the biological cellulose gel product and the product of culture medium preparation in field of food.
Background technique
Biology cellulose (Biocellulose), be otherwise known as bacteria cellulose, is by the microorganism of acetic acid Pseudomonas, such as
Cellulose products made of the fermentation such as acetobacter xylinum.At present mostly with waste, such as coconut water, pineapple peel juice be as raw material, because
This its it is low in cost.Contain up to 95% or more moisture, therefore the biological cellulose hydrogel that is otherwise known as in obtained product.
Domestic bacterial cellulose product makes an addition in food mainly as dietary fiber at present, provides healthcare function to human body.Currently,
The preparation method of biological cellulose gel product mainly includes static culture method and dynamic cultivation method.Static culture method due to
The generation on biological cellulose gel liquid medium within surface, has completely cut off air, leads to aerobic biology cellulose producing strains
In the environment of anoxic can not continued growth breeding, the limits throughput for causing single to produce, production efficiency is relatively low；Dynamically
Cultivation is to improve the dissolved oxygen amount in fluid nutrient medium by mechanical stirring or concussion, but since fluid nutrient medium is continuous
Agitation leads to the mesh space structure relative loose in the biological cellulose gel product generated, this leverages biological fibre
Mechanical performance, mouthfeel, the moisture holding capacity etc. of plain gel products are tieed up, therefore in actual production and is of little use.Currently used life
Fibres element gel entrapment culture base is broadly divided into following a few classes, first is that natural products is directly as culture medium, such as coconut water, useless sugar
The agricultural wastes such as honey, brewex's grains；Second is that human configuration culture medium, uses conventional carbon source (such as glucose, sucrose), nitrogen
Source (peptone, yeast extract, beef extract etc.) and inorganic salts (such as magnesium, calcium) are formulated；Third is that based on natural products
Ingredient is wanted, is additionally added what some nutritional ingredients were formulated.But what present these three types of culture mediums all only considered is it
The effect of nutrition is provided for biological cellulose gel producing strains, not can solve biological cellulose gel culture to certain phase just
It can not be grown due to anoxic and reduce biological cellulose gel and generate quality, thus the problem of reducing taste quality.
Spirulina is a kind of unicellular lower eukaryote, and the filamentous being made of unicellular or many cells is that one kind can be grown on fresh water
Ancient photoautotrophy type microalgae in seawater, can generate oxygen by photosynthesis.And spirulina also has reduction gallbladder
Sterol adjusts blood glucose, strengthen immunity, protection stomach, mends iron, the plurality kinds of health care purposes such as anti-radiation, be it is a kind of can be directly for people
Edible good healthy food material.
Summary of the invention
In view of the above-mentioned problems, present invention firstly provides a kind of biological cellulose gel culture mediums, wherein containing spiral
Algae culturing liquid.
Biological cellulose gel culture medium in the present invention, wherein further comprising carbon source, nitrogen source and inorganic salts.
Biological cellulose gel culture medium in the present invention, is liquid culture medium.
Biological cellulose gel culture medium in the present invention, pH value is in the range of 5.5-7.5, preferably 6.0-6.5.
Biological cellulose gel culture medium in the present invention, spirulina medium therein are the liquid by being improved with viscosity
The preparation of body culture medium culture spirulina.The fluid nutrient medium that the viscosity improves is increased by being added into Spirulina culture medium
Thick dose of acquisition, the thickener is not particularly limited, common various foodstuff glues, such as carragheen, pectin, xanthan gum, Guar
Glue, locust bean gum, Arabic gum, gelatin can use, and dosage is the 0.05-0.1wt% of Spirulina culture medium weight.Institute
The pH value for stating the fluid nutrient medium of viscosity raising is 8.5-10.
Biological cellulose gel culture medium in the present invention, spirulina medium therein are the liquid by being improved with viscosity
Body culture medium, culture preparation in spirulina 3-10 days, was preferably being cultivated under conditions of illumination is sufficient, temperature is 20-35 DEG C
Cheng Zhong keeps pH8-10 with sodium bicarbonate every other day, and supplements urea, and the magnitude of recruitment of urea is the 0.001- of culture medium weight
0.01wt%。
Biological cellulose gel culture medium provided by the invention, is prepared by the following method:
1) Spirulina culture medium that preparation viscosity improves: thickener is added into normal spiral algae culture medium, additional amount is
The 0.05-0.1wt% of Spirulina culture medium weight；
2) cultivate spirulina: being inoculated with spirulina seed in the Spirulina culture medium that Xiang Shangshu viscosity improves, illumination it is sufficient,
Temperature is cultivated spirulina 3-10 days under conditions of being 20-35 DEG C, and during the cultivation process, sodium bicarbonate keeps pH8-10 every other day, and
Urea is supplemented, the magnitude of recruitment of urea is the 0.001-0.01wt% of culture medium weight, obtains spirulina medium；
3) carbon source, nitrogen source and inorganic the preparation of biological cellulose gel culture medium: are added in Xiang Shangshu spirulina medium
Salt, and adjust pH value to 5.5-7.5, biological cellulose gel culture medium is made.
There is no strict requirements, spirulinas for the above-mentioned amount that carbon source, nitrogen source and inorganic salts are added into spirulina medium
The weight ratio of culture solution and carbon source, nitrogen source and inorganic salts can be with are as follows: 1000:(25-40): (10-20): (5-10).
The present invention also provides it is a kind of it is being prepared with above-mentioned biological cellulose gel culture medium, be wherein enclosed with spirulina
Biological cellulose gel product is by generating to above-mentioned biological cellulose gel inoculation of medium biology cellulose
Bacterium, the preparation in static culture 3-12 days at 28-32 DEG C.
Above-mentioned biology cellulose producing strains are gluconate pyracetobacillus or acetobacter xylinum.
After above-mentioned culture, washed repeatedly after solid-liquid separation biological cellulose gel product with sig water and deionized water
Wash biological cellulose gel product.
The present invention also provides above-mentioned biological cellulose gel products to prepare the purposes in food.Above-mentioned food is
Beverage, dairy products, jelly production, bakery product or meat products.
Biological cellulose gel culture medium provided by the invention, wherein spirulina medium is contained, since spirulina exists
Oxygen can be discharged by the photosynthesis of itself when growth, therefore the dissolved oxygen amount in the spirulina medium can be made to mention significantly
Height, then the dissolved oxygen amount in the biological cellulose gel culture medium comprising spirulina medium also greatly improves, and is prepared with its static state
When biological cellulose gel product, at the time of biology cellulose producing strains anoxic can be postponed, so that it is solidifying to improve biology cellulose
The yield and quality of glue.In addition, it is enclosed with the spirulina with plurality kinds of health care effect in the biological cellulose gel product of preparation,
Value of the product is further improved, simultaneously because package of the biological cellulose gel to spirulina, can also effectively avoid spiral
The oxidation of algae avoids the loss and discoloration of nutritional ingredient.Spirulina also makes biological cellulose gel product have pure and fresh green,
With good exterior quality.
Specific embodiment
The present invention is further illustrated with comparative experiments With reference to embodiment, but tool hereinafter
Body implementation method is understood not to limitation of the present invention.Those of ordinary skill in the art can show on the basis of the present invention
And various changes and variation that easy insight is made, it should be all within the scope of the present invention.
Embodiment 1: the preparation of spirulina medium
The xanthan gum of 0.07wt% is added into commercially available liquid spirulina culture medium, after mixing evenly high-temperature short-time sterilization,
It adjusts pH value to 9 after cooling, then accesses spirulina seed, be 20-35 DEG C in illumination abundance, temperature using transparent culture vessel
Under conditions of cultivate spirulina 5 days, be made spirulina medium.
Embodiment 2: the preparation of biological cellulose gel culture medium
The spirulina medium 1L prepared in Example 1, be added glucose 30g, yeast extract 15g, magnesium sulfate 3g,
With citric acid tune pH value to 6.0, biological cellulose gel culture medium is made after mixing evenly in disodium hydrogen phosphate 3g.
Embodiment 3: the preparation of biological cellulose gel
The biological cellulose gel culture medium prepared in Example 2 is inoculated with the wooden glucose vinegar bar by the inoculum concentration of 8wt%
Bacterium, at 28-32 DEG C after static culture 3-7 days, after solid-liquid separation biological cellulose gel product, then with sig water and go from
Sub- water washs repeatedly obtains the biological cellulose gel product comprising spirulina.
Embodiment 4: the application of the biological cellulose gel product comprising spirulina
It is solidifying instead of common biology cellulose with the biological cellulose gel product comprising spirulina prepared in embodiment 3
Glue product, using the conventional method of this field, fruit drink product of the preparation comprising biological cellulose gel product, jelly are produced
Product, sour milk products, bakery product and meat products.
Embodiment described above only describe the preferred embodiments of the invention, not to model of the invention
It encloses and is defined, without departing from the spirit of the design of the present invention, this field ordinary engineering and technical personnel is to skill of the invention
The various changes and improvements that art scheme is made, should fall within the scope of protection determined by the claims of the present invention.
Claims (4)
1. a kind of biological cellulose gel culture medium, is prepared by the following method:
1) Spirulina culture medium that preparation viscosity improves: thickener is added into normal spiral algae culture medium, additional amount is common
The 0.05-0.1wt% of Spirulina culture medium weight；
2) it cultivates spirulina: being inoculated with spirulina seed in the Spirulina culture medium that Xiang Shangshu viscosity improves, in illumination abundance, temperature
It is cultivated under conditions of being 20-35 DEG C spirulina 3-10 days, during the cultivation process, sodium bicarbonate keeps pH8-10 every other day, and supplements
Urea, the magnitude of recruitment of urea are the 0.001-0.01wt% of culture medium weight, obtain spirulina medium；
3) carbon source, nitrogen source and inorganic salts, spiral shell the preparation of biological cellulose gel culture medium: are added in Xiang Shangshu spirulina medium
Revolve algae culturing liquid and carbon source, the weight ratio of nitrogen source and inorganic salts are as follows: 1000:(25-40): (10-20): (5-10), and adjust pH value
To 5.5-7.5, biological cellulose gel culture medium is made.
2. a kind of biological cellulose gel product prepared with biological cellulose gel culture medium described in claim 1, special
Sign is: being enclosed with spirulina in biological cellulose gel product, is by solidifying to biology cellulose described in claim 1
Glue inoculation of medium biology cellulose producing strains, and static culture 3-12 days at 28-32 DEG C, then solid-liquid separation biology is fine
Sig water and deionized water is used to wash preparation repeatedly after tieing up plain gel products.
3. biological cellulose gel product according to claim 2, it is characterised in that: biology cellulose producing strains therein
It is gluconate pyracetobacillus or acetobacter xylinum.
4. biological cellulose gel product described in claim 2 or 3 is preparing the purposes in food, wherein the food is
Beverage, dairy products, jelly production, bakery product or meat products.
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| CN111134333A (en) * | 2018-11-06 | 2020-05-12 | 内蒙古蒙牛乳业（集团）股份有限公司 | Food or health product containing probiotic microcapsules and preparation method thereof |
| CN112587712B (en) * | 2020-12-17 | 2022-07-12 | 福建长源纺织有限公司 | Composite bacterial cellulose dressing based on controllable nanofiber space structure and preparation method |
| CN114181887A (en) * | 2021-11-18 | 2022-03-15 | 浙江工商大学 | Method for constructing in-vitro intestinal tract model based on bacterial cellulose composite gel material |
| DE202022101508U1 (en) | 2022-03-22 | 2022-04-11 | Biswaranjan Acharya | Spirulina production system with intelligent circuit |
| CN115124380B (en) * | 2022-06-09 | 2024-02-06 | 承德石油高等专科学校 | Organic-inorganic compound fertilizer with mesoporous core-shell type and preparation method thereof |
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| CN101386877A (en) * | 2008-10-30 | 2009-03-18 | 上海应用技术学院 | Method for preparing bacteria cellulose compound film and application thereof as face pack material |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101386877A (en) * | 2008-10-30 | 2009-03-18 | 上海应用技术学院 | Method for preparing bacteria cellulose compound film and application thereof as face pack material |
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| CO2、NaHCO3对螺旋藻光合放氧和pH值影响的研究;罗通等;《四川师范大学学报（自然科学版）》;19990930;第22卷(第5期);标题 * |
| Overview of bacterial cellulose production and application.;Faezah Esa et al.;《Agriculture and agricultural science procedia》;20141129;第2卷;第113-119页 * |
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Effective date of registration: 20170421 Address after: 570311 Hainan city of Haikou province white pond Xiuying District poverty alleviation Beverly Road Industrial Development Zone No. 19 Applicant after: Hainan Yeguo Foods Co., Ltd. Applicant after: Nanjing Yeguo Food Co.,Ltd. Address before: 570311 Hainan city of Haikou province white pond Xiuying District poverty alleviation Beverly Road Industrial Development Zone No. 19 Applicant before: Hainan Yeguo Foods Co., Ltd. |
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