CN109123628A - A kind of preparation method of apple ferment - Google Patents

A kind of preparation method of apple ferment Download PDF

Info

Publication number
CN109123628A
CN109123628A CN201810979256.XA CN201810979256A CN109123628A CN 109123628 A CN109123628 A CN 109123628A CN 201810979256 A CN201810979256 A CN 201810979256A CN 109123628 A CN109123628 A CN 109123628A
Authority
CN
China
Prior art keywords
apple
fermentation
added
acetic acid
acid bacteria
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810979256.XA
Other languages
Chinese (zh)
Inventor
彭浩
白徐成
雷海峰
张婳
宋欢
党蓓蕾
刘峰军
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shaanxi Tian Ling Group Co Ltd
Original Assignee
Shaanxi Tian Ling Group Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shaanxi Tian Ling Group Co Ltd filed Critical Shaanxi Tian Ling Group Co Ltd
Priority to CN201810979256.XA priority Critical patent/CN109123628A/en
Publication of CN109123628A publication Critical patent/CN109123628A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The invention discloses a kind of preparation methods of apple ferment, and pectase is added to stoning slice apple and cellulase carries out enzymatic hydrolysis filtering, obtains filtrate;Lactobacillus plantarum and lactobacillus acidophilus heat-preservation fermentation is added in a part of filtrate;Another part filtrate adds fruit S. cervisiae and carries out yeast fermentation, adds acetic acid bacteria after yeast ferments and generates alcohol, allows acetic acid bacteria to sufficiently grow and alcohol is converted into acetic acid;By for a long time, stage by stage, the abundant fermentation of multi-cultur es, two parts fermentation liquid is mixed and through filtering, allotment, filling and sterilization obtains apple ferment.The present invention can allow every kind of strain that can sufficiently grow according to process requirement, improve fermentation efficiency, and the nutritive value of fermentating metabolism product is higher.

Description

A kind of preparation method of apple ferment
Technical field
The invention belongs to food fermentation technical fields, are related to the preparation of ferment, especially a kind of preparation side of apple ferment Method.
Background technique
Ferment product originating from Japan, is sent out using fresh vegetables, fruit as raw material through multiple beneficial bacterium using one or more Ferment and the fermented product generated.The effect of ferment product, there are many expression, such as kept intestinal flora balance, helped digest, improve Cell viability, enhancing immunity of organisms, adjusting body fluid acid-base balance etc..
Apple organic acid rich in, can stimulating gastrointestinal wriggle, promote intestinal health;Blood Cholesterol can be reduced Content avoids cholesterol deposition;Apple is conducive to balance cylinder electrolyte rich in elements such as potassium, phosphorus, iron.Apple has cerebrum tonifying to support Blood, tranquilizing effect.Using the ferment that apple is prepared as raw material, the absorption of nutrient in apple is improved by biofermentation Rate has both the physiological function of ferment.
The production technology of ferment respectively has difference, has relevant patent application.Chinese fermentation industry association was at 2016 10 The moon has issued " ferment product classification directive/guide ", specifies the definition of edible ferment;" edible ferment Good Manufacture Practice " has been issued, It specifies ferment producting rule, but regulation not yet is made to fermenting microbe and ferment strength.
In the prior art, ferment production has natural fermented and two kinds of techniques of strain fermentation.Natural fermented is to utilize fruits and vegetables certainly The strain that body carries ferments, and has the advantages that flavor is more natural, but since strain kind is uncontrollable, it is possible to which there are one Determine food safety risk.In order to guarantee food safety, it is necessary to safe strain and technique appropriate be selected to carry out fermenting and producing ferment Element.And the growth conditions that existing a variety of safe strains need is different, when mixed fermentation with various bacterium exists to be inhibited each other, fermentation efficiency Low disadvantage.
Summary of the invention
It is an object of the invention to overcome the above-mentioned prior art, a kind of preparation method of apple ferment is provided, It can allow every kind of strain that can sufficiently grow according to process requirement, improve fermentation efficiency, the nutritive value of fermentating metabolism product It is higher.
The purpose of the present invention is achieved through the following technical solutions:
The preparation method of this apple ferment is added pectase into the apple of stoning slice and cellulase is digested Filtering, after obtaining filtrate, lactobacillus plantarum and lactobacillus acidophilus heat-preservation fermentation is added in a part of filtrate;Another part filtrate adds again Enter fruit S. cervisiae and carry out yeast fermentation, adds acetic acid bacteria after yeast ferments and generates alcohol, make acetic acid bacteria sufficiently raw Alcohol is simultaneously converted into acetic acid by length;By for a long time, stage by stage, the abundant fermentation of multi-cultur es, two parts fermentation liquid is isometric Mixing obtains apple ferment through filtering, allotment, filling and sterilization.
Further, above method specifically includes the following steps:
Pectase is added into stoning slice apple for step 1) and hair is obtained by filtration in cellulase, 40-45 DEG C of heat preservation 3-4h Zymotic fluid;
Lactobacillus plantarum and lactobacillus acidophilus fermentation, heat-preservation fermentation one week at 36-40 DEG C is added in step 2);
Fruit S. cervisiae, heat-preservation fermentation 5-15 days at 28-30 DEG C is added in step 3);
Acetic acid bacteria is added in step 4), keeps the temperature 14-45 days at 28-30 DEG C of room temperature, alcohol is made to be converted into acetic acid;
The liquid that lactic acid bacteria and acetic acid bacteria are fermented is mixed to get mixed fermentation liquid by step 5) in equal volume;
Mixed fermentation stoste is removed suspended matter and thallus by three-stage filtration mode by step 6), is generated after reservation fermentation Bioactive ingredients, it is formulated, canned, the full activity of ferment is kept, finally in 80-100 DEG C of sterilization 15min.
Further, above step 1) in, the additional amount of the pectase and cellulase are as follows: stoning per ton is sliced apple Middle addition 300g-500g.
Further, above step 2) in, the additional amount of the lactobacillus plantarum and lactobacillus acidophilus are as follows: be sliced in stoning The 0.008-0.01% of slice apple total weight is added in apple.
Further, above step 3) in, the additional amount of the fruit S. cervisiae are as follows: in the apple of stoning slice The 0.05-0.1% of slice apple total weight is added.
Further, above step 4) in, the amount of acetic acid bacteria is added are as follows: it is total that slice apple is added in stoning slice apple The 3-5% of weight.
Further, before above method carries out, apple need to be cleaned with pure water and is dried up, be sliced after removing apple core.
Further, in another embodiment of the present invention, pectase is added to stoning slice apple and cellulase need to be in 40- It is digested at 45 DEG C, is filtered after the completion of enzymatic hydrolysis, obtain filtrate;Lactobacillus plantarum and lactobacillus acidophilus are added into filtrate, It ferments one week at 36-40 DEG C of room temperature;Another part filtrate is added fruit S. cervisiae and carries out yeast fermentation 5-15 days, in yeast Fermentation adds acetic acid bacteria after generating alcohol, allows acetic acid bacteria to sufficiently grow under temperature control and alcohol is converted into acetic acid, make to ferment Alcohol content is 0.5% hereinafter, acetic acid content about 16.7g/L in liquid;It is added after finally two kinds of fermentation liquids are mixed in equal volume A certain amount of oligosaccharide debugs mouthfeel, has just obtained apple ferment.The apple ferment lactic acid content about 18.1g/L, total phenol contain Measure about 153mg/L, SOD enzyme activity about 82U/mL.
The invention has the following advantages:
The growth conditions needed the present invention overcomes a variety of safe strains that the prior art is selected is different, mixed fermentation with various bacterium When exist and inhibit each other, the low disadvantage of fermentation efficiency provides a kind of preparation method of apple ferment, allows every kind of strain can be by It is sufficiently grown according to process requirement, improves fermentation efficiency, it is ensured that the full activity of the bioactive ingredients generated after fermentation makes The nutritive value of fermentating metabolism product significantly improves.
Specific embodiment
The preparation method of apple ferment of the invention is that pectase and cellulase progress enzyme is added to stoning slice apple Solution filtering, obtains filtrate.Lactobacillus plantarum and lactobacillus acidophilus heat-preservation fermentation is added in a part of filtrate;Another part filtrate adds again Enter fruit S. cervisiae and carry out yeast fermentation, adds acetic acid bacteria after yeast ferments and generates alcohol, make acetic acid bacteria sufficiently raw Alcohol is simultaneously converted into acetic acid by length;By for a long time, stage by stage, the abundant fermentation of multi-cultur es, two parts fermentation liquid is isometric Mixing obtains apple ferment through filtering, allotment, filling and sterilization.In highly preferred embodiment of the present invention, needed before starting with pure Water is cleaned apple and is dried up, and is sliced after removing apple core.
Method of the invention specifically includes the following steps:
Pectase is added into stoning slicing fruits for step 1) and hair is obtained by filtration in cellulase, 40-45 DEG C of heat preservation 3-4h Zymotic fluid;
Lactobacillus plantarum and lactobacillus acidophilus fermentation, heat-preservation fermentation one week at 36-40 DEG C is added in step 2);
Fruit S. cervisiae, heat-preservation fermentation 5-15 days at 28-30 DEG C is added in step 3);
Fruit vinegar acetic acid bacteria is added in step 4), is incubated at room temperature 14-45 days, alcohol is made to be converted into acetic acid;
The liquid that lactic acid bacteria and acetic acid bacteria are fermented is mixed to get mixed fermentation liquid by step 5);
Mixed fermentation stoste is removed suspended matter and thallus by three-stage filtration mode by step 6), is generated after reservation fermentation Bioactive ingredients, it is formulated and filling, the full activity of ferment is kept, in 80-100 DEG C of sterilizing 15min.
Below with reference to embodiment, the invention will be described in further detail:
Embodiment 1
Appropriate fruit S. cervisiae is added in 1000 grams of apple flakes, heat-preservation fermentation 14 days at 25 DEG C, in fermentation liquid Alcohol content 8%.Appropriate fruit vinegar acetic acid bacteria is added, 28 DEG C heat-preservation fermentation 30 days, alcohol content 1% in fermentation liquid, acetic acid contains Measure 12.5g/L.Be added lactobacillus plantarum, lactobacillus acidophilus, 33 DEG C heat-preservation fermentation 90 days, lactic acid content 6.2g/L.Total phenol contains Measure 230mg/L, SOD enzyme activity 35U/mL.
Embodiment 2
Appropriate fruit S. cervisiae is added in 1000 grams of apple flakes, heat-preservation fermentation 7 days at 28 DEG C, wine in fermentation liquid Smart content 7%.Appropriate fruit vinegar acetic acid bacteria is added, 30 DEG C heat-preservation fermentation 14 days, alcohol content 0.8% in fermentation liquid, acetic acid contains Measure 18.4g/L.Be added lactobacillus plantarum, lactobacillus acidophilus, 35 DEG C heat-preservation fermentation 120 days, lactic acid content 7.2g/L.Total phenol contains Measure 200mg/L, SOD enzyme activity 43U/mL.
Embodiment 3
Appropriate fruit S. cervisiae is added in 1000 grams of apple flakes, heat-preservation fermentation 5 days at 28 DEG C, wine in fermentation liquid Smart content 5%.Appropriate fruit vinegar acetic acid bacteria is added, 28 DEG C heat-preservation fermentation 45 days, alcohol content 0.2% in fermentation liquid, acetic acid contains Measure 19.3g/L.Be added lactobacillus plantarum, lactobacillus acidophilus, 30 DEG C heat-preservation fermentation 180 days, lactic acid content 12.2g/L.Total phenol Content 193mg/L, SOD enzyme activity 51U/mL.
Embodiment 4
Appropriate fruit S. cervisiae is added in 1000 grams of apple flakes, ferments 15 days at room temperature, alcohol in fermentation liquid Content 6%.Appropriate fruit vinegar acetic acid bacteria is added, ferments 60 days in room temperature, alcohol content 1.5% in fermentation liquid, acetic acid content 16.7g/L.Lactobacillus plantarum, lactobacillus acidophilus is added, ferments 360 days at room temperature, lactic acid content 18.1g/L.Total phenol content 153mg/L, SOD enzyme activity 82U/mL.

Claims (9)

1. a kind of preparation method of apple ferment, which is characterized in that pectase and cellulase is added into stoning slice apple Enzymatic hydrolysis filtering is carried out, filtrate is obtained.Lactobacillus plantarum and lactobacillus acidophilus heat-preservation fermentation is added in a part of filtrate;Another part filter Liquid adds fruit S. cervisiae and carries out yeast fermentation, adds acetic acid bacteria after yeast ferments and generates alcohol, allows acetic acid bacteria It sufficiently grows and alcohol is converted into acetic acid;By for a long time, stage by stage, the abundant fermentation of multi-cultur es, by two parts fermentation liquid Isometric mixing obtains apple ferment through filtering, allotment, filling and sterilization.
2. the preparation method of apple ferment according to claim 1, which is characterized in that specifically includes the following steps:
Pectase is added into stoning slice apple for step 1) and fermentation is obtained by filtration in cellulase, 40-45 DEG C of heat preservation 3-4h Liquid;
Lactobacillus plantarum and lactobacillus acidophilus fermentation, heat-preservation fermentation one week at 36-40 DEG C is added in step 2);
Fruit S. cervisiae, heat-preservation fermentation 5-15 days at 28-30 DEG C is added in step 3);
Acetic acid bacteria is added in step 4), keeps the temperature 14-45 days at 28-30 DEG C of room temperature, alcohol is made to be converted into acetic acid;
The liquid that lactic acid bacteria and acetic acid bacteria are fermented is mixed to get mixed fermentation liquid by step 5) in equal volume;
Mixed fermentation stoste is removed suspended matter and thallus by three-stage filtration mode by step 6), retains the biology generated after fermentation Active constituent, it is formulated, canned, the full activity of ferment is kept, finally in 80-100 DEG C of sterilization 15min.
3. the preparation method of apple ferment according to claim 2, which is characterized in that in step 1), the pectase and The additional amount of cellulase are as follows: 300g-500g is added in slice apple per ton.
4. the preparation method of apple ferment according to claim 2, which is characterized in that in step 2), the plant cream bar The additional amount of bacterium and lactobacillus acidophilus are as follows: the 0.008-0.01% of slice apple total weight is added in stoning slice apple.
5. the preparation method of apple ferment according to claim 2, which is characterized in that in step 3), the fruit wine brewing The additional amount of saccharomycete are as follows: the 0.05-0.1% of slice apple total weight is added in stoning slice apple.
6. the preparation method of apple ferment according to claim 2, which is characterized in that in step 4), acetic acid bacteria is added Amount are as follows: the 3-5% of slice apple total weight is added in stoning slice apple.
7. the preparation method of apple ferment according to claim 1, which is characterized in that clean apple with pure water and blow It is dry, it is sliced after removing apple core.
8. the preparation method of apple ferment according to claim 1, which is characterized in that fruit is added into stoning slice apple Glue enzyme and cellulase carry out enzymatic hydrolysis filtering, obtain filtrate.Lactobacillus plantarum and lactobacillus acidophilus heat preservation is added in a part of filtrate Fermentation one week;Another part filtrate is added fruit S. cervisiae and carries out yeast fermentation 10 days or so, ferments in yeast and generates wine Acetic acid bacteria is added after essence, allows acetic acid bacteria to sufficiently grow under temperature control and alcohol is converted into acetic acid, contain alcohol in fermentation liquid Amount hereinafter, pH reaches 3-4, obtains after fermenation raw liquid mix in proportion, according to different mouthfeels, addition is a certain amount of 0.5% Oligosaccharide deploys mouthfeel, through 80-100 DEG C of sterilizing 15min.
9. the preparation method of apple ferment according to claim 7, which is characterized in that the apple ferment lactic acid content is about For 18.1g/L, total phenol content is about 153mg/L, and SOD enzyme activity is about 82U/mL.
CN201810979256.XA 2018-08-27 2018-08-27 A kind of preparation method of apple ferment Pending CN109123628A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810979256.XA CN109123628A (en) 2018-08-27 2018-08-27 A kind of preparation method of apple ferment

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810979256.XA CN109123628A (en) 2018-08-27 2018-08-27 A kind of preparation method of apple ferment

Publications (1)

Publication Number Publication Date
CN109123628A true CN109123628A (en) 2019-01-04

Family

ID=64828107

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810979256.XA Pending CN109123628A (en) 2018-08-27 2018-08-27 A kind of preparation method of apple ferment

Country Status (1)

Country Link
CN (1) CN109123628A (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111034996A (en) * 2019-12-11 2020-04-21 河北农业大学 Preparation method of apple enzyme functional beverage
CN111109591A (en) * 2019-10-25 2020-05-08 新疆红旗坡农业发展集团有限公司 Intestinal microecology high-efficiency reconstruction type apple enzyme and processing technology
CN111165798A (en) * 2020-01-03 2020-05-19 江西仁仁健康产业有限公司 Preparation method of composite fruit and vegetable enzyme and composite fruit and vegetable enzyme
CN112042934A (en) * 2020-08-21 2020-12-08 中国农业科学院农产品加工研究所 Preparation method of apple enzyme liquid and enzyme powder
CN113519831A (en) * 2021-07-13 2021-10-22 江南大学 Fruit enzyme and preparation method thereof
CN115491280A (en) * 2022-10-25 2022-12-20 贵州省中国科学院天然产物化学重点实验室(贵州医科大学天然产物化学重点实验室) Roxburgh rose enzymatic fruit vinegar and preparation process and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080292751A1 (en) * 2005-05-27 2008-11-27 Kabushiki Kaisha Yakult Honsha Lactic Acid Bacteria Fermented Substance and Fermented Milk Food Product Containing the Same
CN103609985A (en) * 2013-10-25 2014-03-05 晶叶(青岛)生物科技有限公司 Fermentation method for production of nutritionally balanced natural fruit enzyme
CN105054054A (en) * 2015-07-09 2015-11-18 华中农业大学 Preparation method of high-SOD-activity fruit and vegetable enzyme solution and fruit and vegetable enzyme powder
CN107156822A (en) * 2017-06-29 2017-09-15 张萌 Improve the preparation method of SOD in fruit ferment

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080292751A1 (en) * 2005-05-27 2008-11-27 Kabushiki Kaisha Yakult Honsha Lactic Acid Bacteria Fermented Substance and Fermented Milk Food Product Containing the Same
CN103609985A (en) * 2013-10-25 2014-03-05 晶叶(青岛)生物科技有限公司 Fermentation method for production of nutritionally balanced natural fruit enzyme
CN105054054A (en) * 2015-07-09 2015-11-18 华中农业大学 Preparation method of high-SOD-activity fruit and vegetable enzyme solution and fruit and vegetable enzyme powder
CN107156822A (en) * 2017-06-29 2017-09-15 张萌 Improve the preparation method of SOD in fruit ferment

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111109591A (en) * 2019-10-25 2020-05-08 新疆红旗坡农业发展集团有限公司 Intestinal microecology high-efficiency reconstruction type apple enzyme and processing technology
US11297864B2 (en) 2019-10-25 2022-04-12 Sdic Zhonglu Fruit Juice Co., Ltd. Apple enzyme for efficient reconstruction of intestinal microecology and processing technology
CN111034996A (en) * 2019-12-11 2020-04-21 河北农业大学 Preparation method of apple enzyme functional beverage
CN111165798A (en) * 2020-01-03 2020-05-19 江西仁仁健康产业有限公司 Preparation method of composite fruit and vegetable enzyme and composite fruit and vegetable enzyme
CN112042934A (en) * 2020-08-21 2020-12-08 中国农业科学院农产品加工研究所 Preparation method of apple enzyme liquid and enzyme powder
CN113519831A (en) * 2021-07-13 2021-10-22 江南大学 Fruit enzyme and preparation method thereof
CN113519831B (en) * 2021-07-13 2022-04-29 江南大学 Fruit enzyme and preparation method thereof
CN115491280A (en) * 2022-10-25 2022-12-20 贵州省中国科学院天然产物化学重点实验室(贵州医科大学天然产物化学重点实验室) Roxburgh rose enzymatic fruit vinegar and preparation process and application thereof

Similar Documents

Publication Publication Date Title
CN109123628A (en) A kind of preparation method of apple ferment
CN103045429B (en) Preparation method of okra wine
CN101270329B (en) Method for preparing high concentration fruit vinegar with liquid state submerged fermentation
CN106942742A (en) A kind of preparation method of fig ferment
CN105167095A (en) Pitaya peel lactic acid fermented beverage and preparation method thereof
CN107334100A (en) Bird's nest composite enzyme and preparation method thereof
CN108967775A (en) A kind of wild cherry certain kind of berries active plant lactacidase fermenting beverage and preparation method thereof
CN102972819A (en) Preparation method for honey vinegar mulberry juice beverage
CN110236046A (en) A kind of high viable type lactobacillus-fermented blueberry pomace beverage and preparation method thereof
CN103610022A (en) Yeast culture solution and full-course low-salt liquid-state normal-temperature soy sauce fermentation process
CN101469305A (en) Medlar fruit vinegar and preparation thereof
CN110179025A (en) Haw fermented liquid preparing process
CN102972713A (en) Onion sauce fermentation production method
CN104164355A (en) Liquid fermentation technique of hawthorn vinegar
CN111035002A (en) Preparation method of saponin rice enzyme
CN113801813A (en) EM (effective microorganisms) suitable for rose fermentation and application process thereof
CN113057274A (en) Pomegranate juice probiotic beverage and preparation method thereof
CN113679035A (en) Noni enzyme powder and preparation method thereof
CN103815468A (en) Vinegar beverage with effects of appetizing and helping digestion
CN105602821B (en) A kind of Lenlinus edodes A black garlic vinegar and a method for preparing the same
CN105695287B (en) A kind of selenium-rich agaric mulberry health care black vinegar
CN105482982B (en) A method of producing high-quality fruit vinegar using navel orange slag multi-cultur es liquid submerged fermentation
CN104312893A (en) Persimmon vinegar making method
CN105647737B (en) A kind of agaric fungus black garlic wine
CN106538915A (en) The method that probiotics fermention fruit and vegetable juice improves related enzyme activity

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20190104

RJ01 Rejection of invention patent application after publication