CN107334100A - Bird's nest composite enzyme and preparation method thereof - Google Patents
Bird's nest composite enzyme and preparation method thereof Download PDFInfo
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- CN107334100A CN107334100A CN201710515819.5A CN201710515819A CN107334100A CN 107334100 A CN107334100 A CN 107334100A CN 201710515819 A CN201710515819 A CN 201710515819A CN 107334100 A CN107334100 A CN 107334100A
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- lactobacillus acidophilus
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- 244000000626 Daucus carota Species 0.000 title claims abstract description 50
- 235000005770 birds nest Nutrition 0.000 title claims abstract description 50
- 235000005765 wild carrot Nutrition 0.000 title claims abstract description 50
- 239000002131 composite material Substances 0.000 title claims abstract description 25
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 24
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 102
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 41
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 36
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 36
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 36
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 36
- 241000589220 Acetobacter Species 0.000 claims abstract description 35
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 claims abstract description 30
- 244000253911 Saccharomyces fragilis Species 0.000 claims abstract description 30
- 235000018368 Saccharomyces fragilis Nutrition 0.000 claims abstract description 30
- 229940031154 kluyveromyces marxianus Drugs 0.000 claims abstract description 30
- 239000002994 raw material Substances 0.000 claims abstract description 26
- 239000000203 mixture Substances 0.000 claims abstract description 24
- 229940081969 saccharomyces cerevisiae Drugs 0.000 claims abstract description 23
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 19
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 19
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 19
- 240000002605 Lactobacillus helveticus Species 0.000 claims abstract description 17
- 235000013967 Lactobacillus helveticus Nutrition 0.000 claims abstract description 17
- 229940054346 lactobacillus helveticus Drugs 0.000 claims abstract description 17
- 244000005700 microbiome Species 0.000 claims abstract description 15
- 238000012545 processing Methods 0.000 claims abstract description 12
- 235000007119 Ananas comosus Nutrition 0.000 claims abstract description 11
- 244000189799 Asimina triloba Species 0.000 claims abstract description 11
- 235000006264 Asimina triloba Nutrition 0.000 claims abstract description 11
- 235000009467 Carica papaya Nutrition 0.000 claims abstract description 11
- 235000005979 Citrus limon Nutrition 0.000 claims abstract description 11
- 244000131522 Citrus pyriformis Species 0.000 claims abstract description 11
- 235000011430 Malus pumila Nutrition 0.000 claims abstract description 11
- 235000015103 Malus silvestris Nutrition 0.000 claims abstract description 11
- 235000009754 Vitis X bourquina Nutrition 0.000 claims abstract description 11
- 235000012333 Vitis X labruscana Nutrition 0.000 claims abstract description 11
- 240000006365 Vitis vinifera Species 0.000 claims abstract description 11
- 235000014787 Vitis vinifera Nutrition 0.000 claims abstract description 11
- 244000099147 Ananas comosus Species 0.000 claims abstract description 9
- 150000001875 compounds Chemical class 0.000 claims abstract description 8
- 238000000855 fermentation Methods 0.000 claims description 23
- 230000004151 fermentation Effects 0.000 claims description 23
- 239000002054 inoculum Substances 0.000 claims description 20
- 239000012530 fluid Substances 0.000 claims description 18
- 238000002156 mixing Methods 0.000 claims description 15
- 241000220225 Malus Species 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 8
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 6
- 241000186660 Lactobacillus Species 0.000 claims description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 5
- 239000012141 concentrate Substances 0.000 claims description 5
- 229940039696 lactobacillus Drugs 0.000 claims description 5
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 claims description 3
- 239000000052 vinegar Substances 0.000 claims description 3
- 235000021419 vinegar Nutrition 0.000 claims description 3
- 235000011054 acetic acid Nutrition 0.000 claims description 2
- 210000000481 breast Anatomy 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 claims 2
- 241000235649 Kluyveromyces Species 0.000 claims 2
- 241000283073 Equus caballus Species 0.000 claims 1
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 abstract description 10
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 abstract description 5
- SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 abstract description 5
- 229960003080 taurine Drugs 0.000 abstract description 5
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 102000004169 proteins and genes Human genes 0.000 abstract description 3
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 3
- 230000000694 effects Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 241000234671 Ananas Species 0.000 description 2
- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 description 2
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
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- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
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- 238000012360 testing method Methods 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/09—Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention proposes a kind of bird's nest composite enzyme and preparation method thereof, including the raw material of following parts by weight is prepared with compound microorganism ferments：0.03~0.05 part of 5~7 parts of pineapple, 2~4 parts of pawpaw, 0.2~0.3 part of grape, 0.2~0.3 part of Chinese grooseberry, 0.2~0.3 part of apple, 0.2~0.3 part of lemon and bird's nest, the complex microorganism are the mixture of the mixture or acetic acid acetobacter of acetic acid acetobacter, lactobacillus acidophilus, Lactobacillus plantarum, kluyveromyces marxianus and saccharomyces cerevisiae, lactobacillus acidophilus, Lactobacillus helveticus, kluyveromyces marxianus and saccharomyces cerevisiae.Its processing without high temperature bird's nest protein structure of the composite enzyme, with regard to the water solubility of bird's nest, bird's nest sialic acid and the absorption efficiency of taurine can be lifted.
Description
Technical field
The invention belongs to ferment technical field, and in particular to a kind of bird's nest composite enzyme and preparation method thereof.
Background technology
Ferment is present in the animal and plant body of all work, is to maintain body normal function, digestion food, repair tissue etc. is raw
A kind of necessary material of life activity.With advancing age with safe diet, living environment the problems such as, the ferment content in human body
In decline year by year, body declines due to the loss of ferment body metabolism speed, easily produce inferior health and chronic disease etc.
Problem, the trend of aging is presented year by year.If without ferment, biochemical reaction will be unable to carry out, and five big nutrients will all become to machine
Body is utterly useless, and biological phenomena will stop.Ferment is supplemented, can allow what all vital movements can be without any confusion in human body to enter
OK, the body moment of let us maintains vigour.
Bird's nest enjoys high praise, proof on the books as high-grade tonic：Just there is the custom of edible bird's nest nourishing since the Ming Dynasty.
The nineties in last century is even more the peak for welcoming bird's nest development, how to eat bird's nest, the utilization rate for how improving bird's nest is always big
The focus that family is talked about.
However, there is not bird's nest to prepare the report of ferment with fruit at present.
The content of the invention
The present invention proposes a kind of bird's nest composite enzyme, its place without high temperature bird's nest protein structure of the composite enzyme
Reason, with regard to the water solubility of bird's nest, bird's nest sialic acid and the absorption efficiency of taurine can be lifted.
The technical proposal of the invention is realized in this way：
A kind of bird's nest composite enzyme, including the raw material of following parts by weight are prepared with compound microorganism ferments：
5~7 parts of pineapple, 2~4 parts of pawpaw, 0.2~0.3 part of grape, 0.2~0.3 part of Chinese grooseberry, 0.2~0.3 part of apple,
0.2~0.3 part of lemon is acetic acid acetobacter, lactobacillus acidophilus, plant breast with 0.03~0.05 part of bird's nest, the complex microorganism
Bacillus, the mixture of kluyveromyces marxianus and saccharomyces cerevisiae or acetic acid acetobacter, lactobacillus acidophilus, Lactobacillus helveticus,
The mixture of kluyveromyces marxianus and saccharomyces cerevisiae.
Preferably, the complex microorganism is acetic acid acetobacter, lactobacillus acidophilus, Lactobacillus plantarum, Marx's Crewe dimension
Total inoculum concentration of the mixture of yeast and saccharomyces cerevisiae, kluyveromyces marxianus and saccharomyces cerevisiae for raw material gross weight 5~
8wt%, total inoculum concentration of acetic acid acetobacter, lactobacillus acidophilus and Lactobacillus plantarum are 5~8wt% of raw material gross weight.
Preferably, the mass ratio of kluyveromyces marxianus and saccharomyces cerevisiae is 1.5:1.9~2.1, it is acetic acid acetobacter, thermophilic
The mass ratio of Lactobacillus lactis and Lactobacillus plantarum is 1:1:2.1~2.3.
Preferably, the complex microorganism is acetic acid acetobacter, lactobacillus acidophilus, Lactobacillus helveticus, Marx's Crewe dimension
Total inoculum concentration of the mixture of yeast and saccharomyces cerevisiae, kluyveromyces marxianus and saccharomyces cerevisiae for raw material gross weight 5~
8wt%, acetic acid acetobacter, lactobacillus acidophilus and total inoculum concentration of Lactobacillus helveticus are 5~8wt% of raw material gross weight.
Preferably, the mass ratio of kluyveromyces marxianus and saccharomyces cerevisiae is 1.5:1.9~2.1, it is acetic acid acetobacter, thermophilic
Lactobacillus lactis and the mass ratio of Lactobacillus helveticus are 1:1:2.3~2.4.
It is a further object to provide a kind of preparation method of bird's nest composite enzyme, comprise the following steps：
1) Feedstock treating：, then will be respectively after pineapple, pawpaw, grape, Chinese grooseberry, apple, lemon and bird's nest wash clean
Carry out smashing processing, remix to obtain mixing pulp；
2) pulp is handled：Step 1) is mixed into pulp, is cooled to 18~20 DEG C rapidly, obtains mixing pulp, then carry out
Matterization processing, adjusts pH to 3.0~3.5；
3) yeast ferments：The mixture of kluyveromyces marxianus and saccharomyces cerevisiae is added into step 2) homogenize process
Fermented in mixing pulp, fermentation temperature is 18~20 DEG C, until pulp pol as little as 10g/L in zymotic fluid, stops fermentation；
4) lactobacillus ferment：Acetic acid acetobacter, lactobacillus acidophilus and Lactobacillus plantarum are added into the zymotic fluid of step 3)
Mixture or acetic acid acetobacter, the mixture of lactobacillus acidophilus and Lactobacillus helveticus fermented, fermentation temperature 26~30
DEG C, until the pol in zymotic fluid is less than 4g/L, total acid terminates fermentation after being more than 8.5；
5) filtering and concentrating：The zymotic fluid that step 4) is obtained carries out low temperature mistake using diatomite filter and cardboard filter
Filter, then vacuum and low temperature concentrates again.
Preferably, total inoculum concentration of the kluyveromyces marxianus in the step 3) and saccharomyces cerevisiae is raw material gross weight
5~8wt%, total inoculum concentration of acetic acid acetobacter, lactobacillus acidophilus and Lactobacillus plantarum in the step 4) is total for raw material
5~8wt% of weight, total inoculum concentration of vinegar bacillus, lactobacillus acidophilus and Lactobacillus helveticus for raw material gross weight 5~
8wt%.
Preferably, the temperature of the low temperature in the step 4) is 10~15 DEG C.
Preferably, the cycles of concentration of the step 4) is 5 times.
Preferably, during the step 2) homogenize process, pulp pol 100g/L is adjusted.
The bird's nest composite enzyme of the present invention needs low-temperature preservation, and cryogenic temperature suggestion is less than 10 DEG C.
Beneficial effects of the present invention：
Its processing without high temperature bird's nest protein structure of the bird's nest composite enzyme of the present invention, the water with regard to bird's nest can be lifted
The absorption efficiency of dissolubility, bird's nest sialic acid and taurine.Bird's nest sialic acid content is not less than in zymotic fluid after fermenting twice
15g/L, content of taurine are not less than 25g/L.
Embodiment
Embodiment 1
A kind of bird's nest composite enzyme, including the raw material of following parts by weight are prepared with compound microorganism ferments：Pineapple 6
0.04 part of part, 3 parts of pawpaw, 0.25 part of grape, 0.25 part of Chinese grooseberry, 0.25 part of apple, 0.25 part of lemon and bird's nest, composite microbial
Thing is the mixture of acetic acid acetobacter, lactobacillus acidophilus, Lactobacillus plantarum, kluyveromyces marxianus and saccharomyces cerevisiae.
Preparation method：
1) Feedstock treating：, then will be respectively after pineapple, pawpaw, grape, Chinese grooseberry, apple, lemon and bird's nest wash clean
Carry out smashing processing, remix to obtain mixing pulp；
2) pulp is handled：Step 1) is mixed into pulp, is cooled to 18 DEG C rapidly, obtains mixing pulp, then homogenized
Processing, adjust pH to 3.5；
3) yeast ferments：By the mixture of kluyveromyces marxianus and saccharomyces cerevisiae, (kluyveromyces marxianus are with making
Total inoculum concentration of brewer yeast is the 5wt% of raw material gross weight, and the mass ratio of kluyveromyces marxianus and saccharomyces cerevisiae is 1.5:
2) add in the mixing pulp of step 2) homogenize process and fermented, fermentation temperature is 18 DEG C, until pulp sugar in zymotic fluid
As little as 10g/L is spent, stops fermentation；
4) lactobacillus ferment：Acetic acid acetobacter, lactobacillus acidophilus and Lactobacillus plantarum are added into the zymotic fluid of step 3)
Mixture (total inoculum concentration of acetic acid acetobacter, lactobacillus acidophilus and Lactobacillus plantarum be raw material gross weight 5wt%, acetic acid
The mass ratio of acetobacter, lactobacillus acidophilus and Lactobacillus plantarum is 1:1:2.2) fermented, 26 DEG C of fermentation temperature, until fermentation
Pol in liquid is less than 4g/L, and total acid terminates fermentation after being more than 8.5；
5) filtering and concentrating：The zymotic fluid that step 4) is obtained is low using 10 DEG C of diatomite filter and cardboard filter progress
Temperature filtering, then vacuum and low temperature concentrates again, and cycles of concentration is 5 times.
Embodiment 2
A kind of bird's nest composite enzyme, including the raw material of following parts by weight are prepared with compound microorganism ferments：Pineapple 5
Part, 2 parts of pawpaw, 0.3 part of grape, 0.3 part of Chinese grooseberry, 0.3 part of apple, 0.3 part of lemon are with 0.05 part of bird's nest, complex microorganism
Acetic acid acetobacter, lactobacillus acidophilus, Lactobacillus helveticus, the mixture of kluyveromyces marxianus and saccharomyces cerevisiae.
Preparation method：
1) Feedstock treating：, then will be respectively after pineapple, pawpaw, grape, Chinese grooseberry, apple, lemon and bird's nest wash clean
Carry out smashing processing, remix to obtain mixing pulp；
2) pulp is handled：Step 1) is mixed into pulp, is cooled to 20 DEG C rapidly, obtains mixing pulp, then homogenized
Processing, adjust pH to 3.0；
3) yeast ferments：By the mixture of kluyveromyces marxianus and saccharomyces cerevisiae, (kluyveromyces marxianus are with making
Total inoculum concentration of brewer yeast is the 8wt% of raw material gross weight, and the mass ratio of kluyveromyces marxianus and saccharomyces cerevisiae is 1.5:
1.9) add in the mixing pulp of step 2) homogenize process and fermented, fermentation temperature is 20 DEG C, until pulp in zymotic fluid
Pol as little as 10g/L, stop fermentation；
4) lactobacillus ferment：Acetic acid acetobacter, lactobacillus acidophilus and Lactobacillus helveticus are added into the zymotic fluid of step 3)
Mixture (acetic acid acetobacter, lactobacillus acidophilus and total inoculum concentration of Lactobacillus helveticus be raw material gross weight 7wt%, acetic acid
Acetobacter, lactobacillus acidophilus and the mass ratio of Lactobacillus helveticus are 1:1:2.4) fermented, 30 DEG C of fermentation temperature, until fermentation
Pol in liquid is less than 4g/L, and total acid terminates fermentation after being more than 8.5；
5) filtering and concentrating：The zymotic fluid that step 4) is obtained is low using 15 DEG C of diatomite filter and cardboard filter progress
Temperature filtering, then vacuum and low temperature concentrates again, and cycles of concentration is 5 times.
Embodiment 3
A kind of bird's nest composite enzyme, including the raw material of following parts by weight are prepared with compound microorganism ferments：
7 parts of pineapple, 4 parts of pawpaw, 0.2 part of grape, 0.2 part of Chinese grooseberry, 0.2 part of apple, 0.2 part of lemon and bird's nest 0.05
Part, complex microorganism is acetic acid acetobacter, lactobacillus acidophilus, Lactobacillus plantarum, kluyveromyces marxianus and saccharomyces cerevisiae
Mixture.
Preparation method：
1) Feedstock treating：, then will be respectively after pineapple, pawpaw, grape, Chinese grooseberry, apple, lemon and bird's nest wash clean
Carry out smashing processing, remix to obtain mixing pulp；
2) pulp is handled：Step 1) is mixed into pulp, is cooled to 20 DEG C rapidly, obtains mixing pulp, then homogenized
Processing, adjust pH to 3.0；
3) yeast ferments：By the mixture of kluyveromyces marxianus and saccharomyces cerevisiae, (kluyveromyces marxianus are with making
Total inoculum concentration of brewer yeast is the 6wt% of raw material gross weight, and the mass ratio of kluyveromyces marxianus and saccharomyces cerevisiae is 1.5:
2.1) add in the mixing pulp of step 2) homogenize process and fermented, fermentation temperature is 18 DEG C, until pulp in zymotic fluid
Pol as little as 10g/L, stop fermentation；
4) lactobacillus ferment：Acetic acid acetobacter, lactobacillus acidophilus and Lactobacillus plantarum are added into the zymotic fluid of step 3)
Mixture (total inoculum concentration of acetic acid acetobacter, lactobacillus acidophilus and Lactobacillus plantarum be raw material gross weight 8wt%, acetic acid
The mass ratio of acetobacter, lactobacillus acidophilus and Lactobacillus plantarum is 1:1:2.1) fermented, 26 DEG C of fermentation temperature, until fermentation
Pol in liquid is less than 4g/L, and total acid terminates fermentation after being more than 8.5；
5) filtering and concentrating：The zymotic fluid that step 4) is obtained is low using 10 DEG C of diatomite filter and cardboard filter progress
Temperature filtering, then vacuum and low temperature concentrates again, and cycles of concentration is 5 times.
Test example
The bird's nest compound ferment of the present invention is shown in Table 1 compared with the advantageous effect of like product.
The bird's nest composite enzyme of the present invention of table 1 and the Indexes Comparison of like product
| Index | Ferment of the present invention | Commercial like product |
| Sialic acid | 20±3g/L | 5~10g/L |
| Taurine | 30±3g/L | 0.7~0.9g/L |
| SOD activity | 198U/ml | 15~20U/ml |
| Proteinase activity | 143U/ml | 13~16U/ml |
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God any modification, equivalent substitution and improvements made etc., should be included in the scope of the protection with principle.
Claims (9)
- A kind of 1. bird's nest composite enzyme, it is characterised in that raw material including following parts by weight and compound microorganism ferments prepare and Into：5~7 parts of pineapple, 2~4 parts of pawpaw, 0.2~0.3 part of grape, 0.2~0.3 part of Chinese grooseberry, 0.2~0.3 part of apple, lemon 0.2~0.3 part is acetic acid acetobacter, lactobacillus acidophilus, plant breast bar with 0.03~0.05 part of bird's nest, the complex microorganism Bacterium, the mixture of kluyveromyces marxianus and saccharomyces cerevisiae or acetic acid acetobacter, lactobacillus acidophilus, Lactobacillus helveticus, horse The mixture of gram this kluyveromyces and saccharomyces cerevisiae.
- 2. bird's nest composite enzyme according to claim 1, it is characterised in that the complex microorganism be acetic acid acetobacter, Lactobacillus acidophilus, Lactobacillus plantarum, the mixture of kluyveromyces marxianus and saccharomyces cerevisiae, kluyveromyces marxianus are with making Total inoculum concentration of brewer yeast is 5~8wt% of raw material gross weight, acetic acid acetobacter, lactobacillus acidophilus and Lactobacillus plantarum it is total Inoculum concentration is 5~8wt% of raw material gross weight.
- 3. bird's nest composite enzyme according to claim 2, it is characterised in that kluyveromyces marxianus and saccharomyces cerevisiae Mass ratio is 1.5:1.9~2.1, the mass ratio of acetic acid acetobacter, lactobacillus acidophilus and Lactobacillus plantarum is 1:1:2.1~2.3.
- 4. bird's nest composite enzyme according to claim 1, it is characterised in that the complex microorganism be acetic acid acetobacter, Lactobacillus acidophilus, Lactobacillus helveticus, the mixture of kluyveromyces marxianus and saccharomyces cerevisiae, kluyveromyces marxianus are with making Total inoculum concentration of brewer yeast is 5~8wt% of raw material gross weight, acetic acid acetobacter, lactobacillus acidophilus and Lactobacillus helveticus it is total Inoculum concentration is 5~8wt% of raw material gross weight.
- 5. bird's nest composite enzyme according to claim 4, it is characterised in that kluyveromyces marxianus and saccharomyces cerevisiae Mass ratio is 1.5:1.9~2.1, acetic acid acetobacter, lactobacillus acidophilus and the mass ratio of Lactobacillus helveticus are 1:1:2.3~2.4.
- 6. the preparation method of bird's nest composite enzyme as claimed in claim 1, it is characterised in that comprise the following steps：1) Feedstock treating：After pineapple, pawpaw, grape, Chinese grooseberry, apple, lemon and bird's nest wash clean, then it will carry out respectively Processing is smashed, remixes to obtain mixing pulp；2) pulp is handled：Step 1) is mixed into pulp, is cooled to 18~20 DEG C rapidly, obtains mixing pulp, then homogenized Processing, adjust pH to 3.0~3.5；3) yeast ferments：The mixture of kluyveromyces marxianus and saccharomyces cerevisiae is added to the mixing of step 2) homogenize process Fermented in pulp, fermentation temperature is 18~20 DEG C, until pulp pol as little as 10g/L in zymotic fluid, stops fermentation；4) lactobacillus ferment：The mixed of acetic acid acetobacter, lactobacillus acidophilus and Lactobacillus plantarum is added into the zymotic fluid of step 3) Compound or acetic acid acetobacter, the mixture of lactobacillus acidophilus and Lactobacillus helveticus are fermented, 26~30 DEG C of fermentation temperature, directly Pol into zymotic fluid is less than 4g/L, and total acid terminates fermentation after being more than 8.5；5) filtering and concentrating：The zymotic fluid that step 4) is obtained carries out filter at low temperature using diatomite filter and cardboard filter, Then vacuum and low temperature concentrates again.
- 7. the preparation method of bird's nest composite enzyme according to claim 6, it is characterised in that mark in the step 3) Total inoculum concentration of this kluyveromyces and saccharomyces cerevisiae is 5~8wt% of raw material gross weight, the acetic acid vinegar bar in the step 4) Total inoculum concentration of bacterium, lactobacillus acidophilus and Lactobacillus plantarum be raw material gross weight 5~8wt%, vinegar bacillus, lactobacillus acidophilus Total inoculum concentration with Lactobacillus helveticus is 5~8wt% of raw material gross weight.
- 8. the preparation method of bird's nest composite enzyme according to claim 6, it is characterised in that the low temperature in the step 4) Temperature be 10~15 DEG C.
- 9. the preparation method of bird's nest composite enzyme according to claim 6, it is characterised in that the concentration of the step 4) times Number is 5 times.
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| CN107788521A (en) * | 2017-12-01 | 2018-03-13 | 高州市客多多农产品开发有限公司 | A kind of pectase liquid |
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