CN107259577A - Probiotic gel grain and preparation method thereof - Google Patents
Probiotic gel grain and preparation method thereof Download PDFInfo
- Publication number
- CN107259577A CN107259577A CN201710616322.2A CN201710616322A CN107259577A CN 107259577 A CN107259577 A CN 107259577A CN 201710616322 A CN201710616322 A CN 201710616322A CN 107259577 A CN107259577 A CN 107259577A
- Authority
- CN
- China
- Prior art keywords
- probiotics
- grain
- gel
- core body
- probiotic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000006041 probiotic Substances 0.000 title claims abstract description 171
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 171
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 69
- 238000002360 preparation method Methods 0.000 title claims abstract description 27
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims abstract description 36
- 230000000694 effects Effects 0.000 claims abstract description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000003292 glue Substances 0.000 claims abstract description 9
- 238000010521 absorption reaction Methods 0.000 claims abstract description 6
- 238000002156 mixing Methods 0.000 claims abstract description 5
- 239000000499 gel Substances 0.000 claims description 120
- 239000000243 solution Substances 0.000 claims description 44
- 241000894006 Bacteria Species 0.000 claims description 32
- 229920001542 oligosaccharide Polymers 0.000 claims description 32
- 150000002482 oligosaccharides Chemical class 0.000 claims description 31
- 235000010413 sodium alginate Nutrition 0.000 claims description 27
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 26
- 239000011259 mixed solution Substances 0.000 claims description 25
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 18
- 230000001580 bacterial effect Effects 0.000 claims description 16
- 238000009833 condensation Methods 0.000 claims description 14
- 230000005494 condensation Effects 0.000 claims description 14
- 229920001817 Agar Polymers 0.000 claims description 12
- 239000008272 agar Substances 0.000 claims description 12
- 239000001913 cellulose Substances 0.000 claims description 10
- 235000010980 cellulose Nutrition 0.000 claims description 10
- 150000001720 carbohydrates Chemical class 0.000 claims description 9
- 229920002678 cellulose Polymers 0.000 claims description 9
- 239000004310 lactic acid Substances 0.000 claims description 9
- 235000014655 lactic acid Nutrition 0.000 claims description 9
- 150000002632 lipids Chemical class 0.000 claims description 9
- 108090000623 proteins and genes Proteins 0.000 claims description 8
- 102000004169 proteins and genes Human genes 0.000 claims description 8
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 7
- 239000000661 sodium alginate Substances 0.000 claims description 7
- 229940005550 sodium alginate Drugs 0.000 claims description 7
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 6
- 238000007711 solidification Methods 0.000 claims description 5
- 230000008023 solidification Effects 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 4
- 241000606125 Bacteroides Species 0.000 claims description 3
- 241000186000 Bifidobacterium Species 0.000 claims description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 3
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 3
- 239000011575 calcium Substances 0.000 claims description 3
- 229910052791 calcium Inorganic materials 0.000 claims description 3
- 230000008859 change Effects 0.000 claims description 3
- 239000000460 chlorine Substances 0.000 claims description 3
- 229910052801 chlorine Inorganic materials 0.000 claims description 3
- 230000018044 dehydration Effects 0.000 claims description 3
- 238000006297 dehydration reaction Methods 0.000 claims description 3
- 241001478240 Coccus Species 0.000 claims description 2
- 241000235342 Saccharomycetes Species 0.000 claims description 2
- 238000004108 freeze drying Methods 0.000 claims description 2
- 239000006193 liquid solution Substances 0.000 claims 2
- 235000013305 food Nutrition 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 description 17
- 235000013365 dairy product Nutrition 0.000 description 13
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 11
- 238000000034 method Methods 0.000 description 10
- 239000000047 product Substances 0.000 description 10
- 239000001110 calcium chloride Substances 0.000 description 9
- 229910001628 calcium chloride Inorganic materials 0.000 description 9
- 201000010099 disease Diseases 0.000 description 9
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 8
- DLRVVLDZNNYCBX-UHFFFAOYSA-N Polydextrose Polymers OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(O)O1 DLRVVLDZNNYCBX-UHFFFAOYSA-N 0.000 description 8
- 108090000765 processed proteins & peptides Proteins 0.000 description 8
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- 230000036541 health Effects 0.000 description 6
- 230000000968 intestinal effect Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 244000057717 Streptococcus lactis Species 0.000 description 5
- 235000014897 Streptococcus lactis Nutrition 0.000 description 5
- 210000001035 gastrointestinal tract Anatomy 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- 230000002265 prevention Effects 0.000 description 5
- 240000006024 Lactobacillus plantarum Species 0.000 description 4
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- 229930003427 Vitamin E Natural products 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 235000009508 confectionery Nutrition 0.000 description 4
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 4
- 244000005709 gut microbiome Species 0.000 description 4
- 229940072205 lactobacillus plantarum Drugs 0.000 description 4
- 235000013336 milk Nutrition 0.000 description 4
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- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 108010010803 Gelatin Proteins 0.000 description 3
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- 235000013871 bee wax Nutrition 0.000 description 3
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- 235000013361 beverage Nutrition 0.000 description 3
- 230000015271 coagulation Effects 0.000 description 3
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- 239000000839 emulsion Substances 0.000 description 3
- 239000003925 fat Substances 0.000 description 3
- 235000019197 fats Nutrition 0.000 description 3
- 239000008273 gelatin Substances 0.000 description 3
- 229920000159 gelatin Polymers 0.000 description 3
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- 150000003722 vitamin derivatives Chemical class 0.000 description 3
- NOOLISFMXDJSKH-UTLUCORTSA-N (+)-Neomenthol Chemical compound CC(C)[C@@H]1CC[C@@H](C)C[C@@H]1O NOOLISFMXDJSKH-UTLUCORTSA-N 0.000 description 2
- LUEWUZLMQUOBSB-FSKGGBMCSA-N (2s,3s,4s,5s,6r)-2-[(2r,3s,4r,5r,6s)-6-[(2r,3s,4r,5s,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5s,6r)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](OC3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-FSKGGBMCSA-N 0.000 description 2
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 2
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 2
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241001608472 Bifidobacterium longum Species 0.000 description 2
- 241001468229 Bifidobacterium thermophilum Species 0.000 description 2
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 2
- 241000206575 Chondrus crispus Species 0.000 description 2
- NOOLISFMXDJSKH-UHFFFAOYSA-N DL-menthol Natural products CC(C)C1CCC(C)CC1O NOOLISFMXDJSKH-UHFFFAOYSA-N 0.000 description 2
- 229920002581 Glucomannan Polymers 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- AYRXSINWFIIFAE-SCLMCMATSA-N Isomaltose Natural products OC[C@H]1O[C@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)[C@@H](O)[C@@H](O)[C@@H]1O AYRXSINWFIIFAE-SCLMCMATSA-N 0.000 description 2
- 241000186660 Lactobacillus Species 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 2
- 229930003316 Vitamin D Natural products 0.000 description 2
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 2
- 229940072056 alginate Drugs 0.000 description 2
- 235000010443 alginic acid Nutrition 0.000 description 2
- 229920000615 alginic acid Polymers 0.000 description 2
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 2
- 239000001768 carboxy methyl cellulose Substances 0.000 description 2
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 2
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 2
- 239000004203 carnauba wax Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- -1 gala Natural products 0.000 description 2
- 239000007863 gel particle Substances 0.000 description 2
- 229940046240 glucomannan Drugs 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- DLRVVLDZNNYCBX-RTPHMHGBSA-N isomaltose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)C(O)O1 DLRVVLDZNNYCBX-RTPHMHGBSA-N 0.000 description 2
- 229940039696 lactobacillus Drugs 0.000 description 2
- 235000010445 lecithin Nutrition 0.000 description 2
- 239000000787 lecithin Substances 0.000 description 2
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- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019155 vitamin A Nutrition 0.000 description 2
- 239000011719 vitamin A Substances 0.000 description 2
- 235000019166 vitamin D Nutrition 0.000 description 2
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- 150000003710 vitamin D derivatives Chemical class 0.000 description 2
- 229940045997 vitamin a Drugs 0.000 description 2
- 229940046008 vitamin d Drugs 0.000 description 2
- 239000001993 wax Substances 0.000 description 2
- 229920001285 xanthan gum Polymers 0.000 description 2
- YIWGJFPJRAEKMK-UHFFFAOYSA-N 1-(2H-benzotriazol-5-yl)-3-methyl-8-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carbonyl]-1,3,8-triazaspiro[4.5]decane-2,4-dione Chemical compound CN1C(=O)N(c2ccc3n[nH]nc3c2)C2(CCN(CC2)C(=O)c2cnc(NCc3cccc(OC(F)(F)F)c3)nc2)C1=O YIWGJFPJRAEKMK-UHFFFAOYSA-N 0.000 description 1
- 235000019737 Animal fat Nutrition 0.000 description 1
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- 241000194103 Bacillus pumilus Species 0.000 description 1
- 241000606124 Bacteroides fragilis Species 0.000 description 1
- 241000186016 Bifidobacterium bifidum Species 0.000 description 1
- 241000186012 Bifidobacterium breve Species 0.000 description 1
- 241000186015 Bifidobacterium longum subsp. infantis Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
- 241000588921 Enterobacteriaceae Species 0.000 description 1
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- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
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- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 1
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 1
- 240000001046 Lactobacillus acidophilus Species 0.000 description 1
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 1
- 240000001929 Lactobacillus brevis Species 0.000 description 1
- 235000013957 Lactobacillus brevis Nutrition 0.000 description 1
- 244000199866 Lactobacillus casei Species 0.000 description 1
- 235000013958 Lactobacillus casei Nutrition 0.000 description 1
- 241000186673 Lactobacillus delbrueckii Species 0.000 description 1
- 241000186604 Lactobacillus reuteri Species 0.000 description 1
- 241000218588 Lactobacillus rhamnosus Species 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 241000194020 Streptococcus thermophilus Species 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 150000001480 arabinoses Chemical class 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 229940002008 bifidobacterium bifidum Drugs 0.000 description 1
- 229940004120 bifidobacterium infantis Drugs 0.000 description 1
- 229940009291 bifidobacterium longum Drugs 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 150000001719 carbohydrate derivatives Chemical class 0.000 description 1
- 229920006184 cellulose methylcellulose Polymers 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940032049 enterococcus faecalis Drugs 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 235000021255 galacto-oligosaccharides Nutrition 0.000 description 1
- 150000003271 galactooligosaccharides Chemical class 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000034435 immune system development Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 201000002313 intestinal cancer Diseases 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 1
- 229940017800 lactobacillus casei Drugs 0.000 description 1
- 229940001882 lactobacillus reuteri Drugs 0.000 description 1
- 239000000693 micelle Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000020124 milk-based beverage Nutrition 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
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- 239000012460 protein solution Substances 0.000 description 1
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- 230000001105 regulatory effect Effects 0.000 description 1
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- 230000004083 survival effect Effects 0.000 description 1
- 235000019871 vegetable fat Nutrition 0.000 description 1
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- 239000011718 vitamin C Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Jellies, Jams, And Syrups (AREA)
- Medicinal Preparation (AREA)
Abstract
The present invention relates to field of food, and more particularly to probiotic gel grain and preparation method thereof；It includes absorption thalline and wraps up in glue, wherein, absorption thalline includes mixing carrier with probio thalline, and probiotics core body is made；Wrap up in the outer wrap gel overcoat that glue is included in probiotics core body；The water activity of obtained probiotic gel grain is 0.10 0.99；The preparation method is small to the destructiveness of probiotics, can retain enough active probio thallines, and the probiotic gel grain prepared with the preparation method has good accumulating performance, field planting efficiency high of the probiotics in enteron aisle after eating.
Description
Technical field
The present invention relates to field of food, and more particularly to probiotic gel grain and preparation method thereof.
Background technology
In recent years scientific research shows that intestinal microflora structure is not only relevant with common disease of digestive tract, and and suppression
The diseases such as strongly fragrant disease, diabetes, hypertension, obesity, intestinal cancer, inflammation are related, also with child immune system development, antibiotics resistance
The health factors such as property, oncotherapy effect are closely related.Probiotics can be right by setting up rational human enteric bacteria group structure
Associated disease plays the effect of prevention and auxiliary treatment, and beneficial effect is played to health.But traditional probiotics production
Product have that microbial activity is low, viable bacteria rate is low, field planting effect is poor in enteron aisle, and the only defect such as low temperature accumulating.New probiotics phase
Although close product as lyophilized bacterium powder, bacterium piece can normal temperature storage, not anti-digestive juice effect do not improve intestinal colonisation rate yet
Function；Probiotics capsule has certain anti-digestion, but mouthfeel and usage experience are poor, limit probiotic products
Effective utilization and popularization.
The content of the invention
It is an object of the invention to provide a kind of probiotic gel grain, this probiotic gel grain has anti-digestive juice, can
Probiotics is colonized in enteron aisle, the number of viable contributed in eater's enteron aisle increases, and the bacterium vigor in enteron aisle is high, can
The reasonable layout of intestinal microflora is obviously improved, intestinal tissue health is improved, the related disease of prevention and treatment gut flora is assisted
Disease；Probiotic gel grain also has in good taste, the advantage such as resistance to accumulating；The probiotics, which coagulates grain, can also be applied to emulsion, fruits and vegetables
In the solid product such as the liquid form products such as juice and soft sweets, pudding, ice cream.
Another object of the present invention is to provide a kind of preparation method of probiotic gel grain, the preparation method is to probiotics
Activity destruction it is small, can retain enough active probio thallines, and the probiotics prepared with the preparation method is solidifying
Micelle has good accumulating performance, field planting efficiency high of the probiotics in enteron aisle after eating.
The present invention is solved its technical problem and realized using following technical scheme.
The present invention proposes a kind of probiotic gel grain, including probiotics core body and gel overcoat, and gel overcoat is wrapped in
The outside of probiotics core body, probiotics core body includes probio thalline and carrier, and the water activity of probiotic gel grain is
0.10-0.99。
The present invention proposes a kind of preparation method of probiotic gel grain, and it includes absorption thalline and wraps up in glue, wherein, adhered bacteria
Body includes mixing carrier with probio thalline, and probiotics core body is made；Wrap up in the outside bag that glue is included in probiotics core body
Wrap up in gel overcoat.
The embodiment of the present invention probiotic gel grain and preparation method thereof beneficial effect be：The preparation method is to probiotics
Activity destruction it is small, enough active probio thallines can be retained；The probiotic gel prepared by the preparation method
Grain has certain anti-digestive juice function, can efficiently be colonized in enteron aisle probiotics, contribute in eater's enteron aisle
Number of viable increases, and the bacterium vigor in enteron aisle is high, can be obviously improved the reasonable layout of intestinal microflora, improves enteron aisle group
Health is knitted, prevention and treatment gut flora relevant disease is assisted；The probiotic gel grain also have it is in good taste, resistance to accumulating etc. is excellent
Gesture；The probiotics, which coagulates grain, can also be applied to the solid products such as liquid form product and soft sweets, pudding, ice cream such as emulsion, Juice
In.
Embodiment
, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical scheme be clearly and completely described.Unreceipted actual conditions person, builds according to normal condition or manufacturer in embodiment
The condition of view is carried out.Agents useful for same or the unreceipted production firm person of instrument, are the conventional production that can be obtained by commercially available purchase
Product.
Probiotic gel grain of the embodiment of the present invention and preparation method thereof is specifically described below.
The probiotic gel grain of the present invention includes probiotics core body and gel overcoat, and gel overcoat is wrapped in probiotics
The outside of core body.Gel layer outside probiotics core body can be 1 layer, 2 layers, 3 layers, 4 layers etc., not make specific limit herein
It is fixed.The average particle size range of probiotic gel grain is 0.05-15mm, and preferably 0.5-5mm, its shape can be ball-type, four directions
Shape or irregular shape, also it is not specifically limited herein.
Above-mentioned probiotics core body includes probio thalline and carrier.Probio thalline includes Bifidobacterium, lactic acid bacteria, ball
At least one of bacterium, bacillus, saccharomycete and bacteroid, you can so that a kind of strain is only used alone or uses a variety of simultaneously
Strain, specifically, can be selected according to specific effect of probiotic gel grain etc..
Preferably, above-mentioned Bifidobacterium can be bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, youth pair
Discrimination bacillus, bifidobacterium bifidum or bifidobacterium thermophilum；Lactic acid bacteria (lactobacillus) can be lactobacillus acidophilus, Lactobacillus plantarum,
Lactobacillus brevis, Lactobacillus casei, Lactobacillus delbrueckii, Lactobacillus rhamnosus, lactobacillus reuteri or lactobacillus cellobiosas；Coccus
Can be enterococcus faecalis, VREF, streptococcus thermophilus or streptococcus lactis；Bacillus can be bacillus licheniformis, withered grass
Bacillus, bacillus pumilus or solidification bacillus；Bacteroid can be bacteroides fragilis.
It should be noted that the probiotics used in probiotic gel grain is not that can only use above-mentioned probiotics, only
If all probiotics strains that Food and Drug Administration and Chinese food FAD think safe can be selected.
Also need to further illustrate, prepare the probiotic gel grain when, can select two kinds and it is two or more not
The probiotics belonged to together is combined, and different strains can also be selected in same category.
Above-mentioned carrier includes at least one of carbohydrate, protein and lipid, you can be that one of which material is used as load
Body, can also be two or more material as carrier.The use of carrier can effectively lift the probiotic gel
Probiotics in grain and improves number of viable in enteron aisle in the field planting rate of human body intestinal canal, and spawn activity is high, and then can be efficient
Ground promotes the distribution rational in infrastructure of eater's intestinal flora, improves intestinal tissue health, assists prevention and treatment enterobacteriaceae faciation
Related disorders.
Further, carbohydrate can be converted starch, oligosaccharide, cellulose or carbohydrate derivative；Protein can be many
Peptide；Lipid can be animal fat, vegetable fat or lipoid.
Yet further, carbohydrate can be alginate, oligosaccharide, Glucomannan, polydextrose, D-sorbite, agar,
Carragheen, xanthans, bacteria cellulose, carboxymethyl cellulose or vitamin C；Protein can be gelatin or polypeptide；Lipid can
To be animal and plant fat, lecithin, beeswax, insect wax, spermaceti, palm wax, glycerophosphatide, sphingomyelin, menthol, vitamin
A, vitamin D or vitamin E.
It should be noted that when exceeding a kind of as the material of probiotic gel grain carrier, in being allied substances
Two kinds and the material of the above used as carrier, can also be the material of two kinds in inhomogeneity material and the above as carrier
Use.
Above-mentioned gel overcoat includes at least one of carbohydrate, protein and lipid.Preferably, can be converted starch,
At least one of oligosaccharide, cellulose, polypeptide, animal and plant fat, lipoid.
It is further preferred that carbohydrate can be SKGM, alginate, Glucomannan, the sweet sugar of polydextrose, gala,
Rhamnose, FOS, xylo-oligosaccharide, oligoisomaltose, D- xyloses, arabinose, galactooligosaccharide, soyabean oligosaccharides,
D-sorbite, agar, carragheen, xanthans, bacteria cellulose or carboxymethyl cellulose；Protein can be gelatin or polypeptide；
Lipid can be animal and plant fat, lecithin, beeswax, insect wax, spermaceti, palm wax, glycerophosphatide, sphingomyelin, menthol,
Vitamin A, vitamin D or vitamin E.
Gel overcoat can give the protective effect of probiotics core body protrusion, obtained probiotic gel grain is had more
Prominent resistance to accumulating performance, and in the outer wrap gel overcoat of probiotics core body, probiotic gel grain can be improved in people
The colonization ability of body enteron aisle, and then the balance of eater's beneficial bacteria of intestinal tract group is helped lend some impetus to, help to prevent, treat enteron aisle disease
Disease.
The preparation method of probiotics core body can be carried out in accordance with the following methods：
Probio thalline is adsorbed, is mixed on carrier, then solid particle is made, you can above-mentioned probiotics core is made
Body.
In detail, first probiotics can be activated, bred, obtain probiotics bacterial liquid, by the molten of probiotics bacterial liquid and carrier
Liquid is mixed, and solid particle is made, and it is 5 × 10 to obtain thalline content8-2×109CFU/g probiotics core body.Carrier is molten
Liquid can be at least one of saccharide solution, protein solution and lipid solution, and the mass concentration of carrier solution can be
1-5%.The above-mentioned specific method that solid particle is made can be the mixture cryogenic condensation by bacterium solution and carrier solution, for example：
The mixed liquor of agar and oligosaccharide is mixed with bacterium solution, in -2~-7 DEG C, 10~30min of condensation；It can also be probiotics bacterial
(containing sodium alginate), the solidification that probiotics core body is realized in calcium chloride solution, calcium chloride was added dropwise in the mixed liquor of liquid and carrier solution
The mass concentration of solution can be 3-15%.
The preparation of probiotics core body can also be that first (granular size can be by probiotics bacterial liquid and bacteria cellulose grain
3 × 3 × 3mm) absorption mixing, can also further after probiotics bacterial liquid and bacteria cellulose are mixed again with carrier solution
Mixing, is made solid particle.
Above-mentioned probiotics is activated, propagation can for example be carried out in such a way：Probiotics is inoculated in zymotic fluid,
Temperature be 36-38 DEG C, shaking speed be the 16-18h that ferments under the conditions of 110-130rpm.The concentration of bacterium solution after fermentation can be
5×109-2×1011CFU/mL。
Preferably, after probiotics activation, propagation, low-temperature centrifugation can be carried out and wash what is concentrated with buffer solution
Thalline, the thalline after concentration has certain value for preservation, and facilitates the concentration for regulating and controlling bacterium solution in subsequent handling, reaches preparation
Bacterial concentration needed for probiotic gel grain.
It can be carried out in such a way in the method for the outer wrap gel overcoat of probiotics core body：
According to suitable proportions gel overcoat solution, the probiotics core body prepared is positioned over gel overcoat molten
Soaked in liquid, then the probiotics core body coagulation forming of gel overcoat by after immersion, outer wrap, you can probiotics is made
Gel particles.The time soaked in gel overcoat solution can be more than or equal to 5min.
In detail, above-mentioned gel overcoat solution be by least one of carbohydrate, protein and lipid be watered after it is obtained,
Its mass concentration can be 1-20%, for example can 2wt% sodium alginate and 10wt% oligosaccharide constitute.
Further, the above-mentioned process for soaking probiotics core body coagulation forming again in gel overcoat solution can carry out many
Secondary repetition, be for example repeated 2 times, 3 times, it is 4 inferior.Coagulation forming can be that the probiotics core body that will be soaked is placed in calcium chloride
Solidification is more than or equal to 20min in solution (concentration can be 3-15%), can also be cryogenic condensation etc..
To be repeated 3 times, as the mixture of sodium alginate and oligosaccharide, (wherein, sodium alginate is gel overcoat solution
3wt%, oligosaccharide is 10wt%) exemplified by, can be following operation specifically：Probiotics core body is placed in gel overcoat
5min is soaked in solution, then is placed in calcium chloride solution and solidifies 20min, simple gel grain is obtained；Simple gel grain is placed in solidifying
5min is soaked in glue outer layer solution, is subsequently placed in calcium chloride solution and solidifies 20min, two-layer gel grain is obtained；By two-layer gel
Grain is placed in gel overcoat solution and soaks 5min, then is placed in calcium chloride solution and solidifies 20min, you can probiotic gel is made
Grain.
It should be noted that when preparing multilayer gel outer layer, every layer of gel layer can select same gel layer solution,
Different gel layer solution can be selected to carry out the preparation of gel layer outer layer.
Also need to further illustrate, the method for preparing gel overcoat can also be：The prebiotic of completion will be prepared in advance
Bacterium core body is soaked in the mixed solution of agar and oligosaccharide, then carries out cryogenic condensation, and the temperature of cryogenic condensation can be -2
~-7 DEG C.
The water activity of obtained probiotic gel grain can be 0.10-0.99, when needing the moisture of probiotic gel grain
, it is necessary to which processing is dried to obtained probiotic gel grain when activity is less than 0.60.Preferably, can from freeze-drying,
At least one of low temperature drying, fluid bed are air-dried and supercritical fluid is dehydrated, until the water activity of probiotic gel grain is small
In 0.60.
Probiotic gel grain of the present invention and preparation method thereof is described in further detail with reference to embodiments.
Embodiment 1
By lactic acid bacterial liquid (1 × 1011CFU/mL, 1mL) mixed with 2wt% sodium alginate soln (100mL), be added dropwise in
3wt% calcium chloride solutions solidify 20min, obtain probiotics core body (thalline content in obtained probiotics core body for 1 ×
109CFU/g)。
Probiotics core body is placed in 2wt% sodium alginates and 10wt% oligosaccharide solutions and mixes 5min, is taken out, then put
20min is solidified in 3wt% calcium chloride solutions, simple gel grain is obtained；Again by simple gel grain be placed in 2wt% sodium alginates and
5min is mixed in 10wt% oligosaccharide solutions, is taken out, is solidified 20min in 3wt% calcium chloride solutions, obtain two-layer gel
Grain；Two-layer gel grain is placed in 2wt% sodium alginates and 10wt% oligosaccharide solutions again and mixes 5min, takes out, is finally placed in
20min is solidified in 3wt% calcium chloride solutions, the probiotic gel grain that water activity is 0.90 is obtained.
Embodiment 2
By lactic acid bacterial liquid (1 × 1011CFU/mL, 1mL) mixed solution with 2wt% oligosaccharide and 1wt% agar
(100mL) is mixed, and the cryogenic condensation 20min under the conditions of -2 DEG C obtains probiotics core body (in obtained probiotics core body
Thalline content is 1 × 109CFU/g)。
Probiotics core body is placed in 5min in 2wt% sodium alginates and 2wt% oligosaccharide solution, taken out, then 4wt%
20min is solidified in calcium chloride solution, simple gel grain is obtained；Simple gel grain is placed in 3wt% sodium alginates and 10wt% is low
5min in glycan mixed solution, takes out, then is placed in 4wt% calcium chloride solutions and solidifies 20min, obtains two-layer gel grain；Again will
Two-layer gel grain is placed in 5min in 3wt% sodium alginates and 10wt% oligosaccharide mixed solutions, takes out, is finally placed in 4wt% chlorine
Change in calcium solution and solidify 20min, obtain the probiotic gel grain that water activity is 0.85.
Embodiment 3
By lactic acid bacterial liquid (1 × 1011CFU/mL, 1mL), bacteria cellulose grain (3 × 3 × 3mm) and 2wt% oligosaccharide
Mixed with the mixed solution (100mL) of 2wt% agar, the cryogenic condensation 30min under the conditions of -7 DEG C obtains probiotics core body
(the thalline content in obtained probiotics core body is 1 × 109CFU/g)。
Probiotics core body is placed in 2wt% sodium alginates and 10wt% oligosaccharide mixed solutions and soaks 5min, is taken out,
It is placed in again in 10% calcium chloride solution and solidifies 20min, obtains simple gel grain；Simple gel grain is placed in 2wt% sodium alginates
With 5min in 10wt% oligosaccharide mixed solutions, take out, be placed in 10% calcium chloride solution and solidify 20min, obtain two-layer gel
Grain；Two-layer gel grain is placed in 5min in 2wt% sodium alginates and 10wt% oligosaccharide mixed solutions, takes out, is finally placed in
20min is solidified in 10% calcium chloride solution, the probiotic gel grain that water activity is 0.82 is obtained.
Embodiment 4
By lactic acid bacterial liquid (1 × 1011CFU/mL, 1mL), bacteria cellulose grain (3 × 3 × 3mm) and 2wt% oligosaccharide
Mixed with the mixed solution (100mL) of 1.5wt% agar, the cryogenic condensation 10min under the conditions of -5 DEG C obtains probiotics core body
(the thalline content in obtained probiotics core body is 1 × 109CFU/g)。
Probiotics core body is placed in 2wt% sodium alginates and 10wt% oligosaccharide mixed solutions and soaks 5min, is taken out,
It is placed in again in 10% calcium chloride solution and solidifies 20min, obtains simple gel grain；Simple gel grain is placed in 2wt% sodium alginates
With 5min in 10wt% oligosaccharide mixed solutions, take out, be placed in 10% calcium chloride solution and solidify 20min, obtain two-layer gel
Grain；Two-layer gel grain is placed in 5min in 2wt% sodium alginates and 10wt% oligosaccharide mixed solutions, takes out, is placed in 10% chlorine
Change in calcium solution and solidify 20min, after taking-up, be freeze-dried in freeze drier, until the water activity of probiotic gel grain
For 0.50.
Embodiment 5
By bifidobacterium longum bacterium solution (2 × 1011CFU/mL, 1mL) mixed with 5wt% polypeptide solution (100mL), at -6 DEG C
Under the conditions of cryogenic condensation 30min, obtain probiotics core body (thalline content in obtained probiotics core body for 2 ×
109CFU/g)。
Probiotics core body is placed in 2wt% sodium alginates, 1wt% arabinoses and 20wt% polypeptide mixed solutions and soaked
5min is steeped, is taken out, then is placed in 5% calcium chloride solution and solidifies 25min, simple gel grain is obtained；Simple gel grain is placed in
6min in 2wt% sodium alginates and 10wt% oligosaccharide mixed solutions, takes out, is placed in 3% calcium chloride solution and solidifies 25min,
Obtain two-layer gel grain；Two-layer gel grain is placed in into 2wt% sodium alginates, 20ppm vitamin Cs and 12wt% xylo-oligosaccharides to mix
10min in solution, takes out, is placed in 10% calcium chloride solution and solidifies 20min, after taking-up, carries out supercritical fluid dehydration, until
The water activity of probiotic gel grain is 0.40.
Embodiment 6
Streptococcus lactis and bifidobacterium thermophilum are mixed into thalline (5 × 1010CFU/mL, 1mL) with 1wt% beeswax solution
(100mL) is mixed, and the cryogenic condensation 10min under the conditions of -3 DEG C obtains probiotics core body (in obtained probiotics core body
Thalline content is 5 × 108CFU/g)。
Probiotics core body is placed in 2wt% sodium alginates, 8wt% FOSs, 1wt% gelatin, 10ppm vitamin
15min is soaked in E and 10wt% glycerophosphatide mixed solutions, is taken out, then is placed in 15% calcium chloride solution and solidifies 30min, is obtained
To the probiotic gel grain that water activity is 0.72.
Embodiment 7
Lactobacillus plantarum, bacillus subtilis and streptococcus lactis are mixed to the bacterium solution (1.5 × 10 of thalline11CFU/mL, 1mL)
Mixed with 2wt% sodium alginates, 2wt% polydextrose, 15wt% polypeptide and 3wt% vitamin E mixed solution (100mL)
Close, the calcium chloride solution that concentration is 10wt% is added dropwise, obtaining probiotics core body, (thalline in obtained probiotics core body contains
Measure as 1.5 × 109CFU/g)。
The isomaltose that probiotics core body is placed in into 2wt% sodium alginates, 10wt% D-sorbite and 3wt% is mixed
6min in solution, takes out, then solidifies 22min in 12wt% calcium chloride solutions, obtains simple gel grain；Simple gel grain is placed in
8min in 2wt% sodium alginates and 7wt% isomaltose mixed solutions, takes out, then is placed in solidification in 8wt% calcium chloride solutions
20min, is finally carrying out low temperature drying (5 DEG C), obtains the probiotic gel grain that water activity is 0.10.
Embodiment 8
Lactobacillus plantarum, bacillus subtilis and streptococcus lactis are mixed to the bacterium solution (1.2 × 10 of thalline11CFU/mL, 1mL)
Mixed with 2wt% sodium alginates, 2wt% polydextrose, 15wt% polypeptide and 3wt% vitamin E mixed solution (100mL)
Close, the calcium chloride solution that concentration is 10wt% is added dropwise, obtaining probiotics core body, (thalline in obtained probiotics core body contains
Measure as 1.2 × 109CFU/g)。
Probiotics core body is placed in 10min in the mixed solution of 2wt% agar and 5wt% oligosaccharide, -2 after taking-up
Cryogenic condensation under the conditions of DEG C, obtains the probiotic gel grain that water activity is 0.80.
Embodiment 9
Lactobacillus plantarum, bacillus subtilis and streptococcus lactis are mixed to the bacterium solution (1.5 × 10 of thalline9CFU/mL, 1mL)
Bacteria cellulose grain (3 × 3 × 3mm) is adsorbed in, probiotics core body (the thalline content in obtained probiotics core body is obtained
For 1.5 × 109CFU/g)。
Probiotics core body is placed in 8min in the mixed solution of 2wt% agar and 6wt% oligosaccharide, -7 after taking-up
Condensed under the conditions of DEG C；In the mixed solution for being placed in 1wt% agar, 10ppm vitamin Cs and 5wt% oligosaccharide again, -5 after taking-up
Condensed under the conditions of DEG C, obtain the probiotic gel grain that water activity is 0.99.
Test example 1-9
By dairy products purchased in market are made an addition under the probiotic gel grain aseptic condition of embodiment 1,2,3,6,8,9, (length is guaranteed the quality
Phase Yoghourt, UHT sterile milks and normal temperature milk beverage, these dairy products in itself be free of profitable probliotics) in, addition be dairy products in itself
The 10% of quality, under the conditions of 25 DEG C after storage 30 days, 2h is kept at 37 DEG C, according to《GB4789.35》Method detection probiotics
The content (needing to be detected again after probiotic gel grain is crushed) of probiotics, the results are shown in Table 1 in gel particles.
After the probiotic gel grain of embodiment 4,5,7 is preserved 120 days under the conditions of 25 DEG C, city is made an addition under aseptic condition
It is multiple in the dairy products (yoghourt with long shelf life, UHT sterile milks and normal temperature such as beverage, these dairy products are free of profitable probliotics) of purchase in itself
5~15min of water, and in keeping 2h at 37 DEG C, addition is the 10% of dairy products itself quality, according to《GB4789.35》Side
The content (needing to be detected again after probiotic gel grain is crushed) of probiotics, the results are shown in Table in method detection probiotic gel grain
2。
Test example 10
It is 1 × 10 to prepare thalline content11Breast system purchased in market is made an addition under the lactic acid bacterial liquid of CFU/mL thalline, aseptic condition
In product (yoghourt with long shelf life, UHT sterile milks and normal temperature such as beverage, these dairy products are free of profitable probliotics in itself), addition is
The 1% of dairy products itself quality, under the conditions of 25 DEG C after storage 30 days, 2h is kept at 37 DEG C, according to《GB4789.35》Method inspection
Survey the content of probiotics in dairy products.It the results are shown in Table 1.
Test example 11
It is 1 × 10 to prepare thalline content11Dairy products purchased in market are made an addition under CFU/mL lactic acid bacterial liquid, aseptic condition
In (yoghourt with long shelf life, UHT sterile milks and normal temperature such as beverage, these dairy products are free of profitable probliotics in itself), addition is breast
The 1% of product itself quality, under the conditions of 25 DEG C after storage 120 days, 2h is kept at 37 DEG C, according to《GB4789.35》Method inspection
Survey the content of probiotics in dairy products.It the results are shown in Table 2.
Prebiotic bacterial content (CFU/g or CFU/mL) in the probiotic gel grain of the normal temperature middle or short term of table 1 storage
Prebiotic bacterial content (CFU/g or CFU/mL) in the probiotic gel grain of the normal temperature long term storage of table 2
It can be seen from the result of table 1, probiotic gel grain of the invention is after adding in dairy products purchased in market, at normal temperatures
After storage, the survival rate of probiotics is deposited apparently higher than the probiotics that the probiotics for not carrying out gel parcel is directly added into dairy products
Motility rate, illustrates excellent with higher viable bacteria rate in the probiotics middle or short term at normal temperatures in the probiotic gel grain of the present invention
Gesture.
And it can be seen from the result of table 2, probiotic gel grain of the invention is less than 0.60 by dehydration reduction water activity
Afterwards, in higher viable bacteria rate can be kept after long-time storage under normal temperature, illustrate prebiotic in the probiotic gel grain of the present invention
Bacterium has the advantage of long-term accumulating at normal temperatures.
In summary, the beneficial effect of probiotic gel grain of the embodiment of the present invention and preparation method thereof is：The preparation side
Activity destruction of the method to probiotics is small, can retain enough active probio thallines；Prepared by the preparation method
Probiotic gel grain has anti-digestive juice, probiotics can be colonized in enteron aisle, the viable count contributed in eater's enteron aisle
Amount increases, and the bacterium vigor in enteron aisle is high, can be obviously improved the reasonable layout of intestinal microflora, improves intestinal tissue and is good for
Health, assists prevention and treatment gut flora relevant disease；Probiotic gel grain also has in good taste, the advantage such as resistance to accumulating；Should
Probiotics, which coagulates grain, to be applied in the solid products such as liquid form product and soft sweets, pudding, ice cream such as emulsion, Juice.
Embodiments described above is a part of embodiment of the invention, rather than whole embodiments.The reality of the present invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiment in the present invention, what those of ordinary skill in the art were obtained under the premise of creative work is not made
Every other embodiment, belongs to the scope of protection of the invention.
Claims (10)
1. a kind of probiotic gel grain, it is characterised in that including probiotics core body and gel overcoat, the gel overcoat parcel
In the outside of the probiotics core body, the probiotics core body includes probio thalline and carrier, the probiotic gel
The water activity of grain is 0.10-0.99.
2. probiotic gel according to claim 1 grain, it is characterised in that the probio thalline include Bifidobacterium,
At least one of lactic acid bacteria, coccus, bacillus, saccharomycete and bacteroid.
3. probiotic gel according to claim 1 grain, it is characterised in that the carrier also include carbohydrate, protein and
At least one of lipid.
4. probiotic gel grain according to claim 1, it is characterised in that the thalline content in the probiotics core body
For 5 × 108-2×109CFU/g。
5. probiotic gel grain according to claim 1, it is characterised in that the gel overcoat includes carbohydrate, protein
At least one of with lipid.
6. the preparation method of a kind of probiotic gel grain as described in claim any one of 1-5, it is characterised in that including absorption
Thalline and glue is wrapped up in, wherein,
Adsorbing thalline includes mixing in the carrier and the probio thalline, and the probiotics core body is made；
Wrap up in glue and be included in gel overcoat described in the outer wrap of the probiotics core body.
7. the preparation method of probiotic gel according to claim 6 grain, it is characterised in that described wrap up in also includes after glue
Dry, until the water activity of probiotic gel grain is less than 0.60.
8. the preparation method of probiotic gel grain according to claim 7, it is characterised in that the mode of the drying is selected from
Freeze-drying, low temperature drying, fluid bed air-dry and supercritical fluid dehydration at least one of.
9. the preparation method of probiotic gel grain according to claim 6, it is characterised in that the glue of wrapping up in includes following two
The mode of kind：The probiotics core body is soaked in the mixed solution of sodium alginate and oligosaccharide, then is placed in calcium chloride solution
Solidification；Or, the probiotics core body is soaked in the mixed solution of agar and oligosaccharide, then cryogenic condensation, it is described low
The temperature of temperature condensation is -2~-7 DEG C.
10. the preparation method of probiotic gel grain according to claim 6, it is characterised in that the absorption thalline includes
Three kinds of modes below：The mixed solution of oligosaccharide and sodium alginate is mixed with the probiotics bacterial liquid solution, then is added dropwise in chlorine
Change in calcium solution and solidify；Or, the mixed solution of agar and oligosaccharide is mixed with the probiotics bacterial liquid solution, then low temperature cold
Solidifying, the temperature of the cryogenic condensation is -2~-7 DEG C；Again or, by the probio thalline be adsorbed in bacteria cellulose grain.
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| CN110720638A (en) * | 2019-09-22 | 2020-01-24 | 深圳市多微生保健食品有限公司 | Probiotic powder and production method thereof |
| CN111134261A (en) * | 2018-11-06 | 2020-05-12 | 钟宇光 | Solid beverage rich in active probiotics |
| CN111280449A (en) * | 2020-02-11 | 2020-06-16 | 浙江华康药业股份有限公司 | Intestinal slow-release sugar alcohol additive and preparation method and application thereof |
| CN113475717A (en) * | 2021-05-26 | 2021-10-08 | 南京同仁堂黄山精制药业有限公司 | Preparation method of probiotic composition |
| CN113521013A (en) * | 2020-04-20 | 2021-10-22 | 青岛海益康健康科技有限公司 | Embedded probiotic product with planting effect and preparation process thereof |
| WO2023049693A3 (en) * | 2021-09-24 | 2023-05-04 | Johnson & Johnson Consumer Inc. | Dosage forms for the delivery of a probiotic |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111134261A (en) * | 2018-11-06 | 2020-05-12 | 钟宇光 | Solid beverage rich in active probiotics |
| CN110720638A (en) * | 2019-09-22 | 2020-01-24 | 深圳市多微生保健食品有限公司 | Probiotic powder and production method thereof |
| CN111280449A (en) * | 2020-02-11 | 2020-06-16 | 浙江华康药业股份有限公司 | Intestinal slow-release sugar alcohol additive and preparation method and application thereof |
| WO2021160054A1 (en) * | 2020-02-11 | 2021-08-19 | 浙江华康药业股份有限公司 | Enteral sustained-release sugar alcohol additive, preparation method therefor and application thereof |
| JP2022553097A (en) * | 2020-02-11 | 2022-12-21 | 浙江▲華▼康葯▲業▼股▲フン▼有限公司 | Intestinal Sustained-Release Sugar Alcohol Additive, Method for Producing the Same, and Application |
| JP7368618B2 (en) | 2020-02-11 | 2023-10-24 | 浙江▲華▼康葯▲業▼股▲フン▼有限公司 | Intestinal sustained release sugar alcohol additive and its manufacturing method and application |
| CN113521013A (en) * | 2020-04-20 | 2021-10-22 | 青岛海益康健康科技有限公司 | Embedded probiotic product with planting effect and preparation process thereof |
| CN113521013B (en) * | 2020-04-20 | 2023-02-10 | 青岛海益康健康科技有限公司 | Embedded probiotic product with planting effect and preparation process thereof |
| CN113475717A (en) * | 2021-05-26 | 2021-10-08 | 南京同仁堂黄山精制药业有限公司 | Preparation method of probiotic composition |
| WO2023049693A3 (en) * | 2021-09-24 | 2023-05-04 | Johnson & Johnson Consumer Inc. | Dosage forms for the delivery of a probiotic |
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