CN109259245A - A kind of the probiotics Intestine-lubricatinhealth-care health-care food and preparation method stable by fiber - Google Patents
A kind of the probiotics Intestine-lubricatinhealth-care health-care food and preparation method stable by fiber Download PDFInfo
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- CN109259245A CN109259245A CN201811394926.8A CN201811394926A CN109259245A CN 109259245 A CN109259245 A CN 109259245A CN 201811394926 A CN201811394926 A CN 201811394926A CN 109259245 A CN109259245 A CN 109259245A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/20—Agglomerating; Granulating; Tabletting
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention provides a kind of probiotics Intestine-lubricatinhealth-care health-care food and preparation method stable by fiber.The dietary fibre of puffing, probiotics are mixed with the product of prebiotics mixed fermentation, obtain composite powder, then with the beeswax high pressure of fusing to spray, obtain the probiotics of beeswax cladding, finally it is uniformly mixed with Hericium erinaceus extract, Tang oligosaccharide, dried orange peel, peanut skin, spirulina, gelatinized starch, compacting is to get the probiotic health food stable by fiber.This method adsorbs the dietary fibre puffing of probiotics and prebiotics using beeswax cladding; so that by the stable probiotics of fiber high temperature viability with higher; the auxiliary element of addition can effectively safeguard intestinal health simultaneously; intestines peristalsis can be increased; stomach is set to form protective film; effective coordinating intestines and stomach function, products obtained therefrom is a kind of Intestine-lubricatinhealth-care health-care food of excellent effect.
Description
Technical field
The present invention relates to field of health care products, and in particular to the preparation of probiotics health care product, more particularly to one kind by fiber
Stable probiotics Intestine-lubricatinhealth-care health-care food and preparation method.
Background technique
Stomach refers generally to the stomach and small intestine, large intestine part of digestive system.And stomach and small intestine are the cores of nutrient absorption.Human body
The nutrition needed is almost required by stomach；Stomach, which becomes, digests most important organ.Gastrointestinal disorder can seriously affect human body
Absorption to nutrition, if gastrointestinal function weakens, gastric emptying slows down, and causes abdominal distension, belch and indigestion；Because digestive juice secretion subtracts
Few, intestinal bacilli illness causes abnormal fermentation, causes indigestion.Edible high protein fault, beans, dairy products etc., cause
Abdominal fullness；Abdominal discomfort after meal feeds cold, hard or irritable food and is easy diarrhea or constipation etc..Digestive discomfort to people with
Various troubles are carried out.Therefore stomach conditioning is particularly important.
Probiotics is widely used in food safety and life and health field.Intestinal flora balance is adjusted since it has, dimension
Protect intestinal health, can effective coordinating intestines and stomach function, reduce intestinal pH, reduce lactose tolerance, enhancing enteron aisle anti-infection ability,
The effects of enhancing Effects on local immunological functions, improving peristalsis of the digest tract and absorb.Therefore, probiotics is in the health food for enteron aisle
Occupy consequence.But in the production of probiotics, storing process, keep the number of viable of probiotics that can just produce setting good
Enteron aisle health-care effect.Prebiotics and probiotics, are often total to by common growth and active function in order to promote probiotics
With using, this is also the important development direction of current probiotics health care product.
Chinese invention patent application number 201610977930.1 discloses a kind of probiotics health care product, by following parts by weight
Raw material is made: 60 ~ 100 parts of S.hemiphyllum, plumage arteries and veins open country 40 ~ 80 parts of flower extract of fan, sky blue sanicle extract 30 ~
60 parts, 45 ~ 80 parts of Freeze-dry Powder of Probioctics, the antifatigue effect of the invention is good and easy to use.
Chinese invention patent application number 201610144053.X discloses a kind of probiotic granulate, includes following parts by weight
Raw material: 20 ~ 30 parts of lotus root starch, 30 ~ 40 parts of inulin, 20 ~ 30 parts of plant enzyme powder, 10 ~ 15 parts of maltodextrin, 10 ~ 15 parts of pectin, low
10 ~ 15 parts of xylan, 5 ~ 10 parts of oligoisomaltose and 0.1 ~ 1 part of citric acid；Probiotic granulate also includes probiotics, relatively
In every gram of raw material, the additive amount of probiotics is 1 × 106~9×106CFU；.
Chinese invention patent application number 201611005217.7 discloses a kind of probiotic powder of relax bowel and defecation, and the ease constipation is logical
Just the raw material of probiotic powder includes the following: 500 ~ 800 parts of polydextrose, 600 ~ 1000 parts of inulin, oligofructose 600 ~ 900
Part, 500 ~ 900 parts of xylo-oligosaccharide, 500 ~ 800 parts of fruit vegetable powder, 100 ~ 200 parts of animal lactic acid Bifidobacterium, bifidobacterium bifidum
100 ~ 200 parts, 100 ~ 200 parts of baby's bifidobacterium longum, 100 ~ 200 parts of Lactobacillus rhamnosus, 100 ~ 200 parts of lactobacillus acidophilus,
100 ~ 200 parts of bifidobacterium adolescentis, 100 ~ 200 parts of bifidobacterium longum, 100 ~ 200 parts of streptococcus thermophilus, lactobacillus bulgaricus
100 ~ 200 parts and additive.
Chinese invention patent application number 201711339231.5 discloses probiotics tablets and its preparation of a kind of release of colon
Method.Probiotics preparation is made of material containing probiotics and colon releasable material.Material containing probiotics include probiotics medicinal powder and
Pharmaceutically acceptable auxiliary material, probiotics medicinal powder include bifidobacterium longum, bifidobacterium bifidum, streptococcus thermophilus, acidophilus cream
Bacillus, German-style lactobacillus subspecies bulgaricus one or more.Pharmaceutically acceptable accessory package contains filler, disintegration
The auxiliary material of agent and lubricant is one or more.Colon releasable material includes Pectin calcium, polyacrylic resin, chitosan, straight chain shallow lake
Powder, guar gum, chondroitin sulfate or sodium alginate are one or more.
It is extremely quick to temperature since probiotics belongs to active material according to above-mentioned, in existing scheme probiotics health care product
Sense, in production inventory link, the excessively high survival for being not easy to probiotics of temperature, especially in drink etc., reproduction, fortune
Defeated, storage link is required under refrigerated conditions, exacerbate carrying cost significantly.
Summary of the invention
Wider probiotics health care product is applied for current, extremely sensitive for temperature, high temperature survival rate is low, production and storage
Deposit at high cost, the present invention proposes a kind of probiotics Intestine-lubricatinhealth-care health-care food stable by fiber and preparation method, to effectively improve
The high temperature viability of probiotic health food, and have the function of excellent coordinating intestines and stomach.
Specific technical solution of the present invention is as follows:
A kind of preparation method of the probiotics Intestine-lubricatinhealth-care health-care food stable by fiber, comprising the following steps:
(1) dietary fibre is subjected to supercritical drying, crushes, makes its puffing, the dietary fibre of puffing is made；
(2) by probiotics, 5:1 is mixed by volume with prebiotics, is uniformly dispersed, then at 26 ~ 32 DEG C of fermentation temperature reaction 1 ~
The mixture of probiotics and prebiotics is made in 2h；
(3) dietary fibre of puffing made from step (1) is blended with the mixture that step (2) obtains, ultrasonic disperse, so that
Loose dietary fibre sufficiently adsorbs probiotics and prebiotics, and composite powder is made in freeze-drying；
(4) beeswax is heated to 65 ~ 70 DEG C of fusings, composite powder made from the beeswax of fusing and step (3) is respectively placed in height
Two to spraying machine opposite nozzles are pressed, are liquid nitrogen to spray booth, high pressure is carried out to spray, makes beeswax in fully decentralized composite powder
Surface condensation cladding, is made the probiotics of beeswax cladding；
(5) by beeswax made from step (4) cladding probiotics and Hericium erinaceus extract, Tang oligosaccharide, dried orange peel, peanut skin,
Spirulina, gelatinized starch are uniformly mixed, and are suppressed to get the probiotic health food stable by fiber.
Beeswax has good plastotype, detachment, film forming and waterproof, moisture proof, the anti-oxidation characteristics such as rotten, by conduct
It the important materials of food industry and is used as mould release；And there is natural moisture-proof water resistance, emulsibility and slickness,
The base-material of ointment, ointment and soft capsule is widely used as in medical industry, also in the neck such as Chinese patent drug encapsulation and medical sustained release agent
Domain plays huge effect, edible to be used as medicine；The present invention will adsorb the edible of profitable probliotics and prebiotics using beeswax
Fiber cladding can protect probiotics to exempt from using the ability of the good filming protective of beeswax and high latent heat barrier external high temperature
External high temperature influences, and improves high temperature viability.In addition, the component of the adjustable functions of intestines and stomach of addition, can be improved the profit of health care product
Intestines performance.
Preferably, step (1) dietary fibre is one of celery dry powder, wheat bran.
Preferably, carbon dioxide, ammonia, methanol, one in ethyl alcohol can be used in the medium of step (1) described supercritical drying
Kind.
Preferably, step (2) probiotics is lactic acid Bifidobacterium, lactobacillus acidophilus, bifidobacterium breve, De Shi cream bar
At least one of bacterium, Lactobacillus casei.
Preferably, step (2) prebiotics are galactooligosaccharide.
Preferably, in step (3) described composite powder, 60 ~ 65 parts by weight of dietary fibre of puffing, probiotics with it is prebiotic
35 ~ 40 parts by weight of mixture of member.
Preferably, in the probiotics of step (4) beeswax cladding, 8 ~ 12 parts by weight of beeswax of fusing, composite powder 88 ~
92 parts by weight.
Preferably, step (5) gelatinized starch is cornstarch, wheaten starch, starch from sweet potato, potato starch, mung bean
At least one of starch, soybean starch, Rhizoma Nelumbinis starch are made through being gelatinized.
Preferably, in the raw material of step (5) described probiotic health food, 30 ~ 33 parts by weight of probiotics of beeswax cladding,
10 ~ 13 parts by weight of Hericium erinaceus extract, 8 ~ 10 parts by weight of Tang oligosaccharide, 3 ~ 6 parts by weight of dried orange peel, 3 ~ 5 parts by weight of peanut skin, spiral shell
Revolve 10 ~ 14 parts by weight of algae, 19 ~ 36 parts by weight of gelatinized starch.
The present invention also provides the probiotics Intestine-lubricatinhealth-care health-care food stable by fiber that a kind of above-mentioned preparation method is prepared.
By dietary fibre supercritical drying, crushes, make its puffing；Probiotics and prebiotics are uniformly dispersed, it is anti-under fermentation temperature
It answers, subsequently into loose dietary fibre, loose fiber, which sufficiently adsorbs, makes probiotics and prebiotics, is freeze-dried as composite powder
End；Beeswax is heated and is melted, the beeswax of fusing and composite powder are respectively placed in high pressure two to spraying machine opposite nozzles, is led to
High pressure is crossed to spray, beeswax is condensed on fully decentralized composite powder surface and coats, be liquid nitrogen to spray booth, obtain beeswax cladding
Probiotics；The probiotics of beeswax cladding and Hericium erinaceus extract, Tang oligosaccharide, dried orange peel, peanut skin, spirulina, gelatinization are formed sediment
Powder is uniformly mixed, and compacting obtains product.
The present invention provides a kind of probiotics Intestine-lubricatinhealth-care health-care food and preparation method stable by fiber, with prior art phase
Than the feature and excellent effect protruded is:
1, propose that the dietary fibre that absorption profitable probliotics are coated with beeswax prepares the probiotics Intestine-lubricatinhealth-care health-care food stable by fiber
Method.
2, by probiotics and prebiotics then being adsorbed, so that probiotics is steady with prebiotics for dietary fibre puffing
It is fixed, it is further coated using beeswax, the influence using the high latent heat barrier external high temperature of beeswax to probiotics, so that steady by fiber
Fixed probiotics high temperature viability with higher, from being reduced in 30 ~ 40 DEG C of activity.
3, Hericium erinaceus extract, Tang oligosaccharide, dried orange peel, peanut skin, the spirulina that auxiliary is added in present invention preparation, can
Effectively to safeguard intestinal health, intestines peristalsis can be increased, stomach is made to form protective film, effective coordinating intestines and stomach function is obtained
Product is a kind of Intestine-lubricatinhealth-care health-care food of excellent effect.
Specific embodiment
In the following, the present invention will be further described in detail by way of specific embodiments, but this should not be interpreted as to the present invention
Range be only limitted to example below.Without departing from the idea of the above method of the present invention, according to ordinary skill
The various replacements or change that knowledge and customary means are made, should be included in the scope of the present invention.
Embodiment 1
Preparation process are as follows:
(1) dietary fibre is subjected to supercritical drying, crushes, makes its puffing, the dietary fibre of puffing is made；Dietary fibre
For celery dry powder；The medium of supercritical drying uses carbon dioxide；
(2) by probiotics, 5:1 is mixed by volume with prebiotics, is uniformly dispersed, and then reacts 1.5h at 29 DEG C of fermentation temperature,
The mixture of probiotics and prebiotics is made；Probiotics is lactobacillus acidophilus；
(3) dietary fibre of puffing made from step (1) is blended with the mixture that step (2) obtains, ultrasonic disperse, so that
Loose dietary fibre sufficiently adsorbs probiotics and prebiotics, and composite powder is made in freeze-drying；In composite powder, puffing
63 parts by weight of dietary fibre, 37 parts by weight of mixture of probiotics and prebiotics；
(4) beeswax is heated to 67 DEG C of fusings, composite powder made from the beeswax of fusing and step (3) is respectively placed in high pressure pair
Two opposite nozzles of spraying machine are liquid nitrogen to spray booth, carry out high pressure to spray, make beeswax on fully decentralized composite powder surface
Condensation cladding, is made the probiotics of beeswax cladding；In the probiotics of beeswax cladding, 11 parts by weight of beeswax of fusing, composite powder
89 parts by weight；
(5) by beeswax made from step (4) cladding probiotics and Hericium erinaceus extract, Tang oligosaccharide, dried orange peel, peanut skin,
Spirulina, gelatinized starch are uniformly mixed, and are suppressed to get the probiotic health food stable by fiber；Gelatinized starch is corn shallow lake
Powder is made through being gelatinized；In the raw material of probiotic health food, 31 parts by weight of probiotics of beeswax cladding, 11 weight of Hericium erinaceus extract
Measure part, 9 parts by weight of Tang oligosaccharide, 4 parts by weight of dried orange peel, 4 parts by weight of peanut skin, 12 parts by weight of spirulina, 29 weight of gelatinized starch
Measure part.
Test method are as follows:
Choose lactobacillus acidophilus and health food be prepared Following the procedure of Example 1 as research object, respectively at 30 DEG C, 37 DEG C and
10d is placed under the conditions of 45 DEG C, in initial time, 5d, 10d sample detection viable count；Living stems method is first to place sample
Then 1 ~ 2h at room temperature is added in sodium chloride dilution, mix 60s, to ensure that uniform suspension is consistent, dilute suspension step by step
Liquid is inoculated into the MRS agar that temperature is 30 ~ 45 DEG C respectively and trains by the sample liquid 1mL of each different extension rates in 15min
It supports in base plate, 72h is cultivated under (37 ± 1) DEG C anaerobic condition, choose culture dish of the clump count between 30 ~ 300 and counted
Number calculates viable count.
The viable count of the different temperatures storage test of the probiotic health food of the embodiment 1 measured by the above method is such as
Shown in table 1.
Embodiment 2
Preparation process are as follows:
(1) dietary fibre is subjected to supercritical drying, crushes, makes its puffing, the dietary fibre of puffing is made；Dietary fibre
For wheat bran；The medium of supercritical drying uses ammonia；
(2) by probiotics, 5:1 is mixed by volume with prebiotics, is uniformly dispersed, and then reacts 2h at 26 DEG C of fermentation temperature, system
Obtain the mixture of probiotics and prebiotics；Probiotics is lactobacillus acidophilus；
(3) dietary fibre of puffing made from step (1) is blended with the mixture that step (2) obtains, ultrasonic disperse, so that
Loose dietary fibre sufficiently adsorbs probiotics and prebiotics, and composite powder is made in freeze-drying；In composite powder, puffing
60 parts by weight of dietary fibre, 40 parts by weight of mixture of probiotics and prebiotics；
(4) beeswax is heated to 65 DEG C of fusings, composite powder made from the beeswax of fusing and step (3) is respectively placed in high pressure pair
Two opposite nozzles of spraying machine are liquid nitrogen to spray booth, carry out high pressure to spray, make beeswax on fully decentralized composite powder surface
Condensation cladding, is made the probiotics of beeswax cladding；In the probiotics of beeswax cladding, 8 parts by weight of beeswax of fusing, composite powder 92
Parts by weight；
(5) by beeswax made from step (4) cladding probiotics and Hericium erinaceus extract, Tang oligosaccharide, dried orange peel, peanut skin,
Spirulina, gelatinized starch are uniformly mixed, and are suppressed to get the probiotic health food stable by fiber；Gelatinized starch is wheat shallow lake
Powder is made through being gelatinized；In the raw material of probiotic health food, 30 parts by weight of probiotics of beeswax cladding, 10 weight of Hericium erinaceus extract
Measure part, 8 parts by weight of Tang oligosaccharide, 3 parts by weight of dried orange peel, 3 parts by weight of peanut skin, 10 parts by weight of spirulina, 36 weight of gelatinized starch
Measure part.
Test method are as follows:
Choose lactobacillus acidophilus and health food be prepared Following the procedure of Example 2 as research object, respectively at 30 DEG C, 37 DEG C and
10d is placed under the conditions of 45 DEG C, in initial time, 5d, 10d sample detection viable count；Living stems method is first to place sample
Then 1 ~ 2h at room temperature is added in sodium chloride dilution, mix 60s, to ensure that uniform suspension is consistent, dilute suspension step by step
Liquid is inoculated into the MRS agar that temperature is 30 ~ 45 DEG C respectively and trains by the sample liquid 1mL of each different extension rates in 15min
It supports in base plate, 72h is cultivated under (37 ± 1) DEG C anaerobic condition, choose culture dish of the clump count between 30 ~ 300 and counted
Number calculates viable count.
The viable count of the different temperatures storage test of the probiotic health food of the embodiment 2 measured by the above method is such as
Shown in table 1.
Embodiment 3
Preparation process are as follows:
(1) dietary fibre is subjected to supercritical drying, crushes, makes its puffing, the dietary fibre of puffing is made；Dietary fibre
For celery dry powder；The medium of supercritical drying uses methanol；
(2) by probiotics, 5:1 is mixed by volume with prebiotics, is uniformly dispersed, and then reacts 1h at 32 DEG C of fermentation temperature, system
Obtain the mixture of probiotics and prebiotics；Probiotics is lactobacillus acidophilus；
(3) dietary fibre of puffing made from step (1) is blended with the mixture that step (2) obtains, ultrasonic disperse, so that
Loose dietary fibre sufficiently adsorbs probiotics and prebiotics, and composite powder is made in freeze-drying；In composite powder, puffing
65 parts by weight of dietary fibre, 35 parts by weight of mixture of probiotics and prebiotics；
(4) beeswax is heated to 70 DEG C of fusings, composite powder made from the beeswax of fusing and step (3) is respectively placed in high pressure pair
Two opposite nozzles of spraying machine are liquid nitrogen to spray booth, carry out high pressure to spray, make beeswax on fully decentralized composite powder surface
Condensation cladding, is made the probiotics of beeswax cladding；In the probiotics of beeswax cladding, 12 parts by weight of beeswax of fusing, composite powder
88 parts by weight；
(5) by beeswax made from step (4) cladding probiotics and Hericium erinaceus extract, Tang oligosaccharide, dried orange peel, peanut skin,
Spirulina, gelatinized starch are uniformly mixed, and are suppressed to get the probiotic health food stable by fiber；Gelatinized starch is sweet potato shallow lake
Powder is made through being gelatinized；In the raw material of probiotic health food, 33 parts by weight of probiotics of beeswax cladding, 13 weight of Hericium erinaceus extract
Measure part, 10 parts by weight of Tang oligosaccharide, 6 parts by weight of dried orange peel, 5 parts by weight of peanut skin, 14 parts by weight of spirulina, 19 weight of gelatinized starch
Measure part.
Test method are as follows:
Choose lactobacillus acidophilus and health food be prepared Following the procedure of Example 3 as research object, respectively at 30 DEG C, 37 DEG C and
10d is placed under the conditions of 45 DEG C, in initial time, 5d, 10d sample detection viable count；Living stems method is first to place sample
Then 1 ~ 2h at room temperature is added in sodium chloride dilution, mix 60s, to ensure that uniform suspension is consistent, dilute suspension step by step
Liquid is inoculated into the MRS agar that temperature is 30 ~ 45 DEG C respectively and trains by the sample liquid 1mL of each different extension rates in 15min
It supports in base plate, 72h is cultivated under (37 ± 1) DEG C anaerobic condition, choose culture dish of the clump count between 30 ~ 300 and counted
Number calculates viable count.
The viable count of the different temperatures storage test of the probiotic health food of the embodiment 3 measured by the above method is such as
Shown in table 1.
Embodiment 4
Preparation process are as follows:
(1) dietary fibre is subjected to supercritical drying, crushes, makes its puffing, the dietary fibre of puffing is made；Dietary fibre
For wheat bran；The medium of supercritical drying uses ethyl alcohol；
(2) by probiotics, 5:1 is mixed by volume with prebiotics, is uniformly dispersed, and then reacts 2h at 27 DEG C of fermentation temperature, system
Obtain the mixture of probiotics and prebiotics；Probiotics is lactobacillus acidophilus；
(3) dietary fibre of puffing made from step (1) is blended with the mixture that step (2) obtains, ultrasonic disperse, so that
Loose dietary fibre sufficiently adsorbs probiotics and prebiotics, and composite powder is made in freeze-drying；In composite powder, puffing
60 ~ 65 parts by weight of dietary fibre, 35 ~ 40 parts by weight of mixture of probiotics and prebiotics；
(4) beeswax is heated to 65 ~ 70 DEG C of fusings, composite powder made from the beeswax of fusing and step (3) is respectively placed in height
Two to spraying machine opposite nozzles are pressed, are liquid nitrogen to spray booth, high pressure is carried out to spray, makes beeswax in fully decentralized composite powder
Surface condensation cladding, is made the probiotics of beeswax cladding；In the probiotics of beeswax cladding, 9 parts by weight of beeswax of fusing, composite powder
Last 91 parts by weight；
(5) by beeswax made from step (4) cladding probiotics and Hericium erinaceus extract, Tang oligosaccharide, dried orange peel, peanut skin,
Spirulina, gelatinized starch are uniformly mixed, and are suppressed to get the probiotic health food stable by fiber；Gelatinized starch is potato shallow lake
Powder is made through being gelatinized；In the raw material of probiotic health food, 31 parts by weight of probiotics of beeswax cladding, 11 weight of Hericium erinaceus extract
Measure part, 9 parts by weight of Tang oligosaccharide, 4 parts by weight of dried orange peel, 3 parts by weight of peanut skin, 11 parts by weight of spirulina, 31 weight of gelatinized starch
Measure part.
Test method are as follows:
Choose lactobacillus acidophilus and health food be prepared Following the procedure of Example 4 as research object, respectively at 30 DEG C, 37 DEG C and
10d is placed under the conditions of 45 DEG C, in initial time, 5d, 10d sample detection viable count；Living stems method is first to place sample
Then 1 ~ 2h at room temperature is added in sodium chloride dilution, mix 60s, to ensure that uniform suspension is consistent, dilute suspension step by step
Liquid is inoculated into the MRS agar that temperature is 30 ~ 45 DEG C respectively and trains by the sample liquid 1mL of each different extension rates in 15min
It supports in base plate, 72h is cultivated under (37 ± 1) DEG C anaerobic condition, choose culture dish of the clump count between 30 ~ 300 and counted
Number calculates viable count.
The viable count of the different temperatures storage test of the probiotic health food of the embodiment 4 measured by the above method is such as
Shown in table 1.
Embodiment 5
Preparation process are as follows:
(1) dietary fibre is subjected to supercritical drying, crushes, makes its puffing, the dietary fibre of puffing is made；Dietary fibre
For celery dry powder；The medium of supercritical drying uses carbon dioxide；
(2) by probiotics, 5:1 is mixed by volume with prebiotics, is uniformly dispersed, and then reacts 1h at 31 DEG C of fermentation temperature, system
Obtain the mixture of probiotics and prebiotics；Probiotics is lactobacillus acidophilus；
(3) dietary fibre of puffing made from step (1) is blended with the mixture that step (2) obtains, ultrasonic disperse, so that
Loose dietary fibre sufficiently adsorbs probiotics and prebiotics, and composite powder is made in freeze-drying；In composite powder, puffing
64 parts by weight of dietary fibre, 36 parts by weight of mixture of probiotics and prebiotics；
(4) beeswax is heated to 69 DEG C of fusings, composite powder made from the beeswax of fusing and step (3) is respectively placed in high pressure pair
Two opposite nozzles of spraying machine are liquid nitrogen to spray booth, carry out high pressure to spray, make beeswax on fully decentralized composite powder surface
Condensation cladding, is made the probiotics of beeswax cladding；In the probiotics of beeswax cladding, 11 parts by weight of beeswax of fusing, composite powder
89 parts by weight；
(5) by beeswax made from step (4) cladding probiotics and Hericium erinaceus extract, Tang oligosaccharide, dried orange peel, peanut skin,
Spirulina, gelatinized starch are uniformly mixed, and are suppressed to get the probiotic health food stable by fiber；Gelatinized starch is mung bean shallow lake
Powder is made through being gelatinized；In the raw material of probiotic health food, 32 parts by weight of probiotics of beeswax cladding, 12 weight of Hericium erinaceus extract
Measure part, 10 parts by weight of Tang oligosaccharide, 5 parts by weight of dried orange peel, 4 parts by weight of peanut skin, 13 parts by weight of spirulina, 24 weight of gelatinized starch
Measure part.
Test method are as follows:
Choose lactobacillus acidophilus and health food be prepared Following the procedure of Example 5 as research object, respectively at 30 DEG C, 37 DEG C and
10d is placed under the conditions of 45 DEG C, in initial time, 5d, 10d sample detection viable count；Living stems method is first to place sample
Then 1 ~ 2h at room temperature is added in sodium chloride dilution, mix 60s, to ensure that uniform suspension is consistent, dilute suspension step by step
Liquid is inoculated into the MRS agar that temperature is 30 ~ 45 DEG C respectively and trains by the sample liquid 1mL of each different extension rates in 15min
It supports in base plate, 72h is cultivated under (37 ± 1) DEG C anaerobic condition, choose culture dish of the clump count between 30 ~ 300 and counted
Number calculates viable count.
The viable count of the different temperatures storage test of the probiotic health food of the embodiment 5 measured by the above method is such as
Shown in table 1.
Embodiment 6
Preparation process are as follows:
(1) dietary fibre is subjected to supercritical drying, crushes, makes its puffing, the dietary fibre of puffing is made；Dietary fibre
For wheat bran；The medium of supercritical drying uses ethyl alcohol；
(2) by probiotics, 5:1 is mixed by volume with prebiotics, is uniformly dispersed, and then reacts 1.5h at 29 DEG C of fermentation temperature,
The mixture of probiotics and prebiotics is made；Probiotics is lactobacillus acidophilus；
(3) dietary fibre of puffing made from step (1) is blended with the mixture that step (2) obtains, ultrasonic disperse, so that
Loose dietary fibre sufficiently adsorbs probiotics and prebiotics, and composite powder is made in freeze-drying；In composite powder, puffing
62 parts by weight of dietary fibre, 38 parts by weight of mixture of probiotics and prebiotics；
(4) beeswax is heated to 68 DEG C of fusings, composite powder made from the beeswax of fusing and step (3) is respectively placed in high pressure pair
Two opposite nozzles of spraying machine are liquid nitrogen to spray booth, carry out high pressure to spray, make beeswax on fully decentralized composite powder surface
Condensation cladding, is made the probiotics of beeswax cladding；In the probiotics of beeswax cladding, 10 parts by weight of beeswax of fusing, composite powder
90 parts by weight；
(5) by beeswax made from step (4) cladding probiotics and Hericium erinaceus extract, Tang oligosaccharide, dried orange peel, peanut skin,
Spirulina, gelatinized starch are uniformly mixed, and are suppressed to get the probiotic health food stable by fiber；Gelatinized starch is beautiful soybean
Starch is made through being gelatinized；In the raw material of probiotic health food, 32 parts by weight of probiotics of beeswax cladding, Hericium erinaceus extract 12
Parts by weight, 9 parts by weight of Tang oligosaccharide, 4 parts by weight of dried orange peel, 4 parts by weight of peanut skin, 12 parts by weight of spirulina, gelatinized starch 27
Parts by weight.
Test method are as follows:
Choose lactobacillus acidophilus and health food be prepared Following the procedure of Example 6 as research object, respectively at 30 DEG C, 37 DEG C and
10d is placed under the conditions of 45 DEG C, in initial time, 5d, 10d sample detection viable count；Living stems method is first to place sample
Then 1 ~ 2h at room temperature is added in sodium chloride dilution, mix 60s, to ensure that uniform suspension is consistent, dilute suspension step by step
Liquid is inoculated into the MRS agar that temperature is 30 ~ 45 DEG C respectively and trains by the sample liquid 1mL of each different extension rates in 15min
It supports in base plate, 72h is cultivated under (37 ± 1) DEG C anaerobic condition, choose culture dish of the clump count between 30 ~ 300 and counted
Number calculates viable count.
The viable count of the different temperatures storage test of the probiotic health food of the embodiment 6 measured by the above method is such as
Shown in table 1.
Comparative example 1
Preparation process are as follows:
(1) dietary fibre is crushed,；Dietary fibre is wheat bran；The medium of supercritical drying uses ethyl alcohol；
(2) by probiotics, 5:1 is mixed by volume with prebiotics, is uniformly dispersed, and then reacts 1.5h at 29 DEG C of fermentation temperature,
The mixture of probiotics and prebiotics is made；Probiotics is lactobacillus acidophilus；
(3) dietary fibre made from step (1) is blended with the mixture that step (2) obtains, ultrasonic disperse, so that loose food
Probiotics and prebiotics are sufficiently adsorbed with fiber, are freeze-dried, composite powder is made；In composite powder, the edible fibre of puffing
Tie up 38 parts by weight of mixture of 62 parts by weight, probiotics and prebiotics；
(4) by composite powder made from step (3) and Hericium erinaceus extract, Tang oligosaccharide, dried orange peel, peanut skin, spirulina, paste
Change starch to be uniformly mixed, suppress to get the probiotic health food stable by fiber；Gelatinized starch is beautiful soybean starch through being gelatinized
It is made；In the raw material of probiotic health food, 32 parts by weight of composite powder, 12 parts by weight of Hericium erinaceus extract, Tang oligosaccharide 9
Parts by weight, 4 parts by weight of dried orange peel, 4 parts by weight of peanut skin, 12 parts by weight of spirulina, 27 parts by weight of gelatinized starch.
Comparative example 1 is not to dietary fibre puffing.
Test method are as follows:
Choose lactobacillus acidophilus according to comparative example 1 method be made health food as research object, respectively at 30 DEG C, 37 DEG C and
10d is placed under the conditions of 45 DEG C, in initial time, 5d, 10d sample detection viable count；Living stems method is first to place sample
Then 1 ~ 2h at room temperature is added in sodium chloride dilution, mix 60s, to ensure that uniform suspension is consistent, dilute suspension step by step
Liquid is inoculated into the MRS agar that temperature is 30 ~ 45 DEG C respectively and trains by the sample liquid 1mL of each different extension rates in 15min
It supports in base plate, 72h is cultivated under (37 ± 1) DEG C anaerobic condition, choose culture dish of the clump count between 30 ~ 300 and counted
Number calculates viable count.
The viable count of the different temperatures storage test of the probiotic health food of the comparative example 1 measured by the above method is such as
Shown in table 1.
Table 1:
Claims (10)
1. a kind of preparation method of the probiotics Intestine-lubricatinhealth-care health-care food stable by fiber, which comprises the following steps:
(1) dietary fibre is subjected to supercritical drying, crushes, makes its puffing, the dietary fibre of puffing is made；
(2) by probiotics, 5:1 is mixed by volume with prebiotics, is uniformly dispersed, then at 26 ~ 32 DEG C of fermentation temperature reaction 1 ~
The mixture of probiotics and prebiotics is made in 2h；
(3) dietary fibre of puffing made from step (1) is blended with the mixture that step (2) obtains, ultrasonic disperse, so that
Loose dietary fibre sufficiently adsorbs probiotics and prebiotics, and composite powder is made in freeze-drying；
(4) beeswax is heated to 65 ~ 70 DEG C of fusings, composite powder made from the beeswax of fusing and step (3) is respectively placed in height
Two to spraying machine opposite nozzles are pressed, are liquid nitrogen to spray booth, high pressure is carried out to spray, makes beeswax in fully decentralized composite powder
Surface condensation cladding, is made the probiotics of beeswax cladding；
(5) by beeswax made from step (4) cladding probiotics and Hericium erinaceus extract, Tang oligosaccharide, dried orange peel, peanut skin,
Spirulina, gelatinized starch are uniformly mixed, and are suppressed to get the probiotic health food stable by fiber.
2. a kind of preparation method of the probiotics Intestine-lubricatinhealth-care health-care food stable by fiber, feature exist according to claim 1
In: step (1) dietary fibre is one of celery dry powder, wheat bran.
3. a kind of preparation method of the probiotics Intestine-lubricatinhealth-care health-care food stable by fiber, feature exist according to claim 1
In: the medium of step (1) described supercritical drying uses one of carbon dioxide, ammonia, methanol, ethyl alcohol.
4. a kind of preparation method of the probiotics Intestine-lubricatinhealth-care health-care food stable by fiber, feature exist according to claim 1
In: step (2) probiotics is lactic acid Bifidobacterium, lactobacillus acidophilus, bifidobacterium breve, Lactobacillus delbrueckii, cheese cream bar
At least one of bacterium.
5. a kind of preparation method of the probiotics Intestine-lubricatinhealth-care health-care food stable by fiber, feature exist according to claim 1
In: step (2) prebiotics are galactooligosaccharide.
6. a kind of preparation method of the probiotics Intestine-lubricatinhealth-care health-care food stable by fiber, feature exist according to claim 1
In: in step (3) described composite powder, 60 ~ 65 parts by weight of dietary fibre of puffing, probiotics and prebiotics mixture 35
~ 40 parts by weight.
7. a kind of preparation method of the probiotics Intestine-lubricatinhealth-care health-care food stable by fiber, feature exist according to claim 1
In: in the probiotics of step (4) the beeswax cladding, 8 ~ 12 parts by weight of beeswax, 88 ~ 92 parts by weight of composite powder of fusing.
8. a kind of preparation method of the probiotics Intestine-lubricatinhealth-care health-care food stable by fiber, feature exist according to claim 1
In: step (5) gelatinized starch is cornstarch, wheaten starch, starch from sweet potato, potato starch, green starch, soybean shallow lake
At least one of powder, Rhizoma Nelumbinis starch are made through being gelatinized.
9. a kind of preparation method of the probiotics Intestine-lubricatinhealth-care health-care food stable by fiber, feature exist according to claim 1
In: in the raw material of step (5) described probiotic health food, 30 ~ 33 parts by weight of probiotics of beeswax cladding, Hericium erinaceus extract
10 ~ 13 parts by weight, 8 ~ 10 parts by weight of Tang oligosaccharide, 3 ~ 6 parts by weight of dried orange peel, 3 ~ 5 parts by weight of peanut skin, 10 ~ 14 weight of spirulina
Measure part, 19 ~ 36 parts by weight of gelatinized starch.
10. a kind of probiotics Intestine-lubricatinhealth-care health-care stable by fiber that any one of claim 1 ~ 9 preparation method is prepared
Food.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110115345A (en) * | 2019-05-10 | 2019-08-13 | 成都大学 | A kind of nutrition digested tankage and preparation method thereof |
| CN115463184A (en) * | 2022-10-08 | 2022-12-13 | 天津小薇生物科技有限公司 | Probiotic-containing composition with function of promoting sleep and preparation method and application thereof |
-
2018
- 2018-11-22 CN CN201811394926.8A patent/CN109259245A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110115345A (en) * | 2019-05-10 | 2019-08-13 | 成都大学 | A kind of nutrition digested tankage and preparation method thereof |
| CN115463184A (en) * | 2022-10-08 | 2022-12-13 | 天津小薇生物科技有限公司 | Probiotic-containing composition with function of promoting sleep and preparation method and application thereof |
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