CN115191611A - Enteric-coated probiotic preparation and preparation method thereof - Google Patents
Enteric-coated probiotic preparation and preparation method thereof Download PDFInfo
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- CN115191611A CN115191611A CN202210835456.4A CN202210835456A CN115191611A CN 115191611 A CN115191611 A CN 115191611A CN 202210835456 A CN202210835456 A CN 202210835456A CN 115191611 A CN115191611 A CN 115191611A
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- 239000006041 probiotic Substances 0.000 title claims abstract description 113
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 113
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 109
- 238000002360 preparation method Methods 0.000 title claims abstract description 59
- 239000003094 microcapsule Substances 0.000 claims abstract description 32
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 26
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000000661 sodium alginate Substances 0.000 claims abstract description 24
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 24
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 24
- 229920001661 Chitosan Polymers 0.000 claims abstract description 21
- 239000011248 coating agent Substances 0.000 claims abstract description 17
- 238000000576 coating method Methods 0.000 claims abstract description 17
- 241000894006 Bacteria Species 0.000 claims description 31
- 239000007788 liquid Substances 0.000 claims description 22
- 238000003756 stirring Methods 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 17
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 15
- 239000008223 sterile water Substances 0.000 claims description 15
- 238000001816 cooling Methods 0.000 claims description 10
- 238000000855 fermentation Methods 0.000 claims description 10
- 230000004151 fermentation Effects 0.000 claims description 10
- 239000002244 precipitate Substances 0.000 claims description 10
- 238000005507 spraying Methods 0.000 claims description 10
- 239000001963 growth medium Substances 0.000 claims description 8
- 239000003595 mist Substances 0.000 claims description 8
- 239000004925 Acrylic resin Substances 0.000 claims description 5
- 229920000178 Acrylic resin Polymers 0.000 claims description 5
- 241000186000 Bifidobacterium Species 0.000 claims description 5
- 241000186660 Lactobacillus Species 0.000 claims description 5
- DOOTYTYQINUNNV-UHFFFAOYSA-N Triethyl citrate Chemical compound CCOC(=O)CC(O)(C(=O)OCC)CC(=O)OCC DOOTYTYQINUNNV-UHFFFAOYSA-N 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 5
- 239000008367 deionised water Substances 0.000 claims description 5
- 229910021641 deionized water Inorganic materials 0.000 claims description 5
- 238000007865 diluting Methods 0.000 claims description 5
- 238000001879 gelation Methods 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 claims description 5
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 claims description 5
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 claims description 5
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 claims description 5
- 229940039696 lactobacillus Drugs 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 239000001069 triethyl citrate Substances 0.000 claims description 5
- VMYFZRTXGLUXMZ-UHFFFAOYSA-N triethyl citrate Natural products CCOC(=O)C(O)(C(=O)OCC)C(=O)OCC VMYFZRTXGLUXMZ-UHFFFAOYSA-N 0.000 claims description 5
- 235000013769 triethyl citrate Nutrition 0.000 claims description 5
- 239000012299 nitrogen atmosphere Substances 0.000 claims description 2
- 238000009423 ventilation Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000002245 particle Substances 0.000 abstract description 8
- 239000001110 calcium chloride Substances 0.000 abstract 1
- 229910001628 calcium chloride Inorganic materials 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 238000004090 dissolution Methods 0.000 description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000002249 digestive system Anatomy 0.000 description 2
- 239000002702 enteric coating Substances 0.000 description 2
- 238000009505 enteric coating Methods 0.000 description 2
- 238000009776 industrial production Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000002253 acid Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 210000004051 gastric juice Anatomy 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/015—Inorganic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/03—Organic compounds
- A23L29/035—Organic compounds containing oxygen as heteroatom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention relates to the technical field of probiotic preparation processing, in particular to an enteric-coated probiotic preparation and a preparation method thereof. The invention provides an enteric-coated probiotic preparation which is high in efficiency and small in microcapsule particle size and a preparation method thereof. The invention carries out microcapsule coating on the probiotics through the gel effect of sodium alginate, chitosan and calcium chloride. The invention forms the probiotic microcapsule by contacting the fog drops of the solution A and the fog drops of the calcium chloride solution, so that the particle size of the probiotic microcapsule is smaller, and the working efficiency is obviously improved.
Description
The technical field is as follows:
the invention relates to the technical field of probiotic preparation processing, in particular to an enteric-coated probiotic preparation and a preparation method thereof.
Background art:
the probiotics is a kind of active microorganism beneficial to a host by colonizing in the host and changing the composition of flora at a certain part of the host, and means that the probiotics is a general name of a single microorganism or mixed microorganism with definite composition which is beneficial to health by regulating the immune function of a host mucous membrane and a system or regulating the balance of flora in an intestinal tract and promoting the nutrient absorption to keep the intestinal tract healthy.
The pH of gastric juice in the stomach of a digestive system is generally 0.9-1.5, and when the probiotic product enters the digestive system of a human body and passes through the stomach, strong acid can kill most of viable bacteria, so that the effectiveness of the probiotic product is greatly reduced, therefore, the method for preparing the probiotics into the microcapsules is provided at present, and the probiotic product is not dissolved in the stomach and is partially dissolved in the intestinal tract through coating the outer layer of the microcapsules, so that the problem of reduction of most of the viable bacteria is avoided.
Sodium alginate is a good enteric coating material, but at present, when sodium alginate is used for microencapsulation, a calcium chloride solution is generally dripped into a sodium alginate solution by an injector through an orifice method to form microcapsules, but the method has low efficiency, and the prepared microcapsules have large particle size and are not suitable for industrial production.
Aiming at the problems, the enteric-coated probiotic preparation which has higher efficiency and smaller microcapsule grain size and the preparation method thereof are provided.
Disclosure of Invention
The invention provides an enteric-coated probiotic preparation and a preparation method thereof, aiming at solving the defects that the existing preparation method of the enteric-coated probiotic preparation is low in efficiency, and the prepared microcapsule is large in particle size and is not suitable for industrial production.
In order to solve the technical problems, the invention provides a preparation method of an enteric-coated probiotic preparation
The method comprises the following steps:
s1: inoculating probiotic strains in an MRS culture medium, and continuously culturing for two generations to obtain probiotic fermentation liquor;
s2: centrifuging the probiotic fermentation liquor for 10min at 500rpm, taking supernatant to obtain probiotic bacteria liquid, and diluting the probiotic bacteria liquid to ensure that the concentration of viable bacteria in the probiotic bacteria liquid is 900-1100cfu/g;
s3: dissolving sodium alginate and chitosan in deionized sterile water, stirring at room temperature to dissolve, and making the mass fraction of sodium alginate and chitosan be 10-30%, to obtain solution A;
s4: adding 3-8% of probiotic bacteria liquid into the solution A, and uniformly stirring to obtain a solution B;
s5: respectively introducing the solution B and the calcium chloride solution into a sprayer, spraying the solution A and the calcium chloride solution by the sprayer face to face, allowing the fog drops of the solution A and the calcium chloride solution to contact for gelation to form probiotic microcapsules, and allowing the probiotic microcapsules to fall on a bearing plate at the bottom,
s6: naturally cooling the probiotic microcapsules to prepare the enteric-coated probiotic preparation.
Further, the probiotic strain is one or two of lactobacillus or bifidobacterium.
Further, the ratio of sodium alginate to chitosan is 1.
Further, the step S3 includes the following steps:
s3.1: heating deionized sterile water to 60-70 deg.C;
s3.2: adding sodium alginate into the heated deionized sterile water, and stirring by using a stirrer until the solution is clear and has no precipitate;
s3.3: c, adding citric acid into the solution obtained in the step b, and adjusting the pH of the solution to 4-5;
s3.4: c, adding chitosan into the solution obtained in the step c, and continuing stirring by using a stirrer until the solution is clear and has no precipitate;
s3.5: and d, naturally cooling the solution obtained in the step d to obtain a solution A.
Further, in the step S4, in the process of adding the probiotic liquid, the whole process is performed in a nitrogen atmosphere.
Further, in the step S5, while the mist of the solution a and the mist of the calcium chloride solution are contacted to gel, ventilation is performed from the bottom upward, so that the mist of the solution a and the mist of the calcium chloride solution are sufficiently mixed.
Further, after the enteric probiotic preparation is prepared in step S6, the method further comprises the following steps:
s7: adding 5-10 parts of acrylic resin, 5-10 parts of triethyl citrate and 0.1-0.5 part of hydroxypropyl methyl cellulose into 50-60 parts of deionized water to prepare a solution C;
s8: and spraying the solution C on the surface of the enteric-coated probiotic preparation through a fluidized bed coating machine, and further coating the enteric-coated probiotic preparation.
Furthermore, the working conditions of the fluidized bed coating machine are that the temperature of an air inlet is 50-60 ℃, the temperature of an air outlet is 40-50 ℃, and the air speed is 3-5m/min.
Drawings
FIG. 1 is a diagram illustrating the composition of specific raw materials in an example of the present invention.
The invention has the following advantages:
1. the invention forms the probiotic microcapsule by contacting the fog drops of the solution A and the fog drops of the calcium chloride solution, so that the particle size of the probiotic microcapsule is smaller, and the working efficiency is obviously improved.
2. According to the invention, the enteric-coated probiotic preparation is further coated in an enteric-coating manner, so that the slow-release performance of the enteric-coated probiotic preparation is obviously improved.
The specific implementation mode is as follows:
example 1
The invention provides a preparation method of an enteric-coated probiotic preparation, which comprises the following steps:
s1: inoculating lactobacillus and bifidobacterium in an MRS culture medium, wherein the MRS culture medium is selected from Qinghai Haibobo biological limited company, and continuously culturing for two generations to obtain probiotic fermentation liquor;
s2: centrifuging the probiotic fermentation liquor for 10min at 500rpm, taking supernatant to obtain probiotic bacteria liquid, and diluting the probiotic bacteria liquid to ensure that the concentration of viable bacteria in the probiotic bacteria liquid is 1000cfu/g;
s3: dissolving sodium alginate and chitosan in deionized sterile water, stirring and dissolving at room temperature to make the mass fraction of sodium alginate and chitosan be 20%, and preparing solution A;
the step S3 specifically includes the following steps:
s3.1: heating deionized sterile water to 60 ℃;
s3.2: adding 10% sodium alginate into the heated deionized sterile water, and stirring by using a stirrer until the solution is clear and has no precipitate;
s3.3: c, adding 1% citric acid into the solution obtained in the step b, adjusting the pH of the solution to 5, reducing the viscosity of sodium alginate and facilitating the subsequent dissolution of chitosan;
s3.4: c, adding 10% chitosan into the solution obtained in the step c, and continuing stirring by using a stirrer until the solution is clear and has no precipitate;
s3.5: and d, naturally cooling the solution obtained in the step d to obtain a solution A.
S4: adding 5% of probiotic bacteria liquid into the solution A in a nitrogen environment to prevent the living bacteria from being exposed to inactivation under an aerobic condition, and uniformly stirring to obtain a solution B;
s5: respectively introducing the solution B and the calcium chloride solution into a sprayer, spraying the solution A and the calcium chloride solution by the sprayer face to face, ventilating from the bottom to the top, fully mixing fog drops of the solution A and fog drops of the calcium chloride solution, and allowing the fog drops of the solution A and the fog drops of the calcium chloride solution to contact to carry out gelation to form probiotic microcapsules which fall on a bearing plate at the bottom, wherein the particle size of the finally formed probiotic microcapsules is small due to the fact that the probiotic microcapsules are contacted in a fog drop state;
s6: naturally cooling the probiotic microcapsules to prepare an enteric probiotic preparation;
s7: adding 10 parts of acrylic resin, 10 parts of triethyl citrate and 0.3 part of hydroxypropyl methyl cellulose into 60 parts of deionized water to prepare a solution C;
s8: and spraying the solution C on the surface of the enteric-coated probiotic preparation through a fluidized bed coating machine, wherein the working conditions of the fluidized bed coating machine are that the air inlet temperature is 60 ℃, the air outlet temperature is 40 ℃, and the air speed is 5m/min, and further coating is carried out on the enteric-coated probiotic preparation.
The embedding rate of the enteric-coated probiotic preparation prepared by the steps is 88.17 percent.
Example 2:
the invention provides a preparation method of an enteric-coated probiotic preparation, which comprises the following steps:
s1: inoculating lactobacillus and bifidobacterium in an MRS culture medium, wherein the MRS culture medium is selected from Qinghai Haibobo biological limited company, and continuously culturing for two generations to obtain probiotic fermentation liquor;
s2: centrifuging the probiotic fermentation liquor for 10min at 500rpm, taking supernatant to obtain probiotic bacteria liquid, and diluting the probiotic bacteria liquid to ensure that the concentration of viable bacteria in the probiotic bacteria liquid is 1000cfu/g;
s3: dissolving sodium alginate and chitosan in deionized sterile water, stirring and dissolving at room temperature to make the mass fraction of sodium alginate and chitosan be 20%, and preparing solution A;
the step S3 specifically includes the following steps:
s3.1: heating deionized sterile water to 60 ℃;
s3.2: adding 10% sodium alginate into the heated deionized sterile water, and stirring by using a stirrer until the solution is clear and has no precipitate;
s3.3: c, adding 1% citric acid into the solution obtained in the step b, adjusting the pH of the solution to 5, reducing the viscosity of sodium alginate and facilitating the subsequent dissolution of chitosan;
s3.4: c, adding 10% chitosan into the solution obtained in the step c, and continuing stirring by using a stirrer until the solution is clear and has no precipitate;
s3.5: and d, naturally cooling the solution obtained in the step d to obtain a solution A.
S4: under the nitrogen environment, adding 15% of probiotic bacteria liquid into the solution A to prevent the living bacteria from being inactivated under the aerobic condition, and uniformly stirring to obtain a solution B;
s5: respectively introducing the solution B and the calcium chloride solution into a sprayer, spraying the solution A and the calcium chloride solution by the sprayer face to face, ventilating from the bottom to the top, fully mixing fog drops of the solution A and fog drops of the calcium chloride solution, and allowing the fog drops of the solution A and the fog drops of the calcium chloride solution to contact to carry out gelation to form probiotic microcapsules, wherein the probiotic microcapsules fall on a bearing plate at the bottom, and the particle size of the finally formed probiotic microcapsules is smaller because the probiotic microcapsules are contacted in a fog drop state;
s6: naturally cooling the probiotic microcapsules to prepare an enteric-coated probiotic preparation;
s7: adding 10 parts of acrylic resin, 10 parts of triethyl citrate and 0.3 part of hydroxypropyl methyl cellulose into 60 parts of deionized water to prepare a solution C;
s8: and spraying the solution C on the surface of the enteric-coated probiotic preparation through a fluidized bed coating machine, wherein the working conditions of the fluidized bed coating machine are that the air inlet temperature is 60 ℃, the air outlet temperature is 40 ℃, and the air speed is 5m/min, and further coating is carried out on the enteric-coated probiotic preparation.
The embedding rate of the enteric-coated probiotic preparation prepared by the steps is 90.03%. Example 3:
the invention provides a preparation method of an enteric-coated probiotic preparation, which comprises the following steps:
s1: inoculating lactobacillus and bifidobacterium into an MRS culture medium, wherein the MRS culture medium is selected from Qinghai Haibo biological limited company, and continuously culturing for two generations to obtain probiotic fermentation liquor;
s2: centrifuging the probiotic fermentation liquor for 10min at 500rpm, taking supernatant to obtain probiotic bacteria liquid, and diluting the probiotic bacteria liquid to ensure that the concentration of viable bacteria in the probiotic bacteria liquid is 1000cfu/g;
s3: dissolving sodium alginate and chitosan in deionized sterile water, stirring and dissolving at room temperature to make the mass fraction of sodium alginate and chitosan be 30%, and preparing solution A;
the step S3 specifically includes the following steps:
s3.1: heating deionized sterile water to 60 ℃;
s3.2: adding 15% sodium alginate into the heated deionized sterile water, and stirring by using a stirrer until the solution is clear and has no precipitate;
s3.3: adding 1% citric acid into the solution obtained in the step b, adjusting the pH of the solution to 5, reducing the viscosity of sodium alginate and facilitating the subsequent dissolution of chitosan;
s3.4: c, adding 15% chitosan into the solution obtained in the step c, and continuing stirring by using a stirrer until the solution is clear and has no precipitate;
s3.5: and d, naturally cooling the solution obtained in the step d to obtain a solution A.
S4: under the nitrogen environment, adding 5% of probiotic bacteria liquid into the solution A to prevent the living bacteria from being inactivated under the aerobic condition, and uniformly stirring to obtain a solution B;
s5: respectively introducing the solution B and the calcium chloride solution into a sprayer, spraying the solution A and the calcium chloride solution by the sprayer face to face, ventilating from the bottom to the top, fully mixing fog drops of the solution A and fog drops of the calcium chloride solution, and allowing the fog drops of the solution A and the fog drops of the calcium chloride solution to contact to carry out gelation to form probiotic microcapsules, wherein the probiotic microcapsules fall on a bearing plate at the bottom, and the particle size of the finally formed probiotic microcapsules is smaller because the probiotic microcapsules are contacted in a fog drop state;
s6: naturally cooling the probiotic microcapsules to prepare an enteric-coated probiotic preparation;
s7: adding 10 parts of acrylic resin, 10 parts of triethyl citrate and 0.3 part of hydroxypropyl methyl cellulose into 60 parts of deionized water to prepare a solution C;
s8: and spraying the solution C on the surface of the enteric-coated probiotic preparation through a fluidized bed coating machine, wherein the working conditions of the fluidized bed coating machine are that the air inlet temperature is 60 ℃, the air outlet temperature is 40 ℃, and the air speed is 5m/min, and further coating is carried out on the enteric-coated probiotic preparation.
The embedding rate of the enteric-coated probiotic preparation prepared by the steps is 89.88%.
Claims (9)
1. A preparation method of an enteric-coated probiotic preparation is characterized by comprising the following steps:
s1: inoculating probiotic strains in an MRS culture medium, and continuously culturing for two generations to obtain probiotic fermentation liquor;
s2: centrifuging the probiotic fermentation liquor for 10min at 500rpm, taking supernatant to obtain probiotic bacteria liquid, and diluting the probiotic bacteria liquid to ensure that the concentration of viable bacteria in the probiotic bacteria liquid is 900-1100cfu/g;
s3: dissolving sodium alginate and chitosan in deionized sterile water, stirring at room temperature to dissolve, and making the mass fraction of sodium alginate and chitosan be 10-30%, to obtain solution A;
s4: adding 3-8% of probiotic bacteria liquid into the solution A, and uniformly stirring to obtain a solution B;
s5: respectively introducing the solution B and the calcium chloride solution into a sprayer, spraying the solution A and the calcium chloride solution by the sprayer face to face, and allowing the fog drops of the solution A and the fog drops of the calcium chloride solution to contact for gelation to form probiotic microcapsules, wherein the probiotic microcapsules fall on a bearing plate at the bottom;
s6: naturally cooling the probiotic microcapsules to prepare the enteric-coated probiotic preparation.
2. The method for preparing an enteric probiotic preparation according to claim 1, characterized in that: the probiotic strain is one or two of lactobacillus or bifidobacterium.
3. The method for preparing an enteric probiotic preparation according to claim 1, characterized in that: the ratio of sodium alginate to chitosan is 1.
4. The method for preparing an enteric probiotic preparation according to claim 1, characterized in that: the step S3 includes the steps of:
s3.1: heating deionized sterile water to 60-70 deg.C;
s3.2: adding sodium alginate into the heated deionized sterile water, and stirring by using a stirrer until the solution is clear and has no precipitate;
s3.3: adding citric acid into the solution obtained in the step b, and adjusting the pH of the solution to 4-5;
s3.4: c, adding chitosan into the solution obtained in the step c, and continuing stirring by using a stirrer until the solution is clear and has no precipitate;
s3.5: and d, naturally cooling the solution obtained in the step d to obtain a solution A.
5. The method for preparing an enteric probiotic preparation according to claim 1, characterized in that: in the step S4, the whole process is carried out in the nitrogen atmosphere in the process of adding the probiotic bacteria liquid.
6. The method for preparing an enteric probiotic preparation according to claim 1, characterized in that: in the step S5, when the mist of the solution a and the mist of the calcium chloride solution are contacted to gel, ventilation is performed from the bottom to the top, so that the mist of the solution a and the mist of the calcium chloride solution are sufficiently mixed.
7. The method for preparing an enteric probiotic preparation according to claim 1, characterized in that: after the enteric probiotic preparation is prepared in the step S6, the method also comprises the following steps:
s7: adding 5-10 parts of acrylic resin, 5-10 parts of triethyl citrate and 0.1-0.5 part of hydroxypropyl methyl cellulose into 50-60 parts of deionized water to prepare a solution C;
s8: and spraying the solution C on the surface of the enteric-coated probiotic preparation through a fluidized bed coating machine, and further coating the enteric-coated probiotic preparation.
8. The method for preparing an enteric probiotic preparation as claimed in claim 7, characterized in that: the working conditions of the fluidized bed coating machine are that the temperature of an air inlet is 50-60 ℃, the temperature of an air outlet is 40-50 ℃, and the air speed is 3-5m/min.
9. An enteric probiotic preparation produced by the method of making an enteric probiotic preparation according to claims 1-8.
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| CN1969889A (en) * | 2006-12-04 | 2007-05-30 | 济南赛拜斯生物工程有限公司 | Enteric-coated multilayer encapsulated probiotic microcapsule and preparation method thereof |
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