CN1965675A

CN1965675A - Feed additive of biological apoenzyme and production technique

Info

Publication number: CN1965675A
Application number: CNA2006100177628A
Authority: CN
Inventors: 郜德顺
Original assignee: Individual
Current assignee: Individual
Priority date: 2006-04-30
Filing date: 2006-04-30
Publication date: 2007-05-23

Abstract

The invention relates to a biological enzyme forage additive and relative production, wherein said method comprises that: via mother seed activation, triangle bottle bacterial, curtain seed cultivation, preparing beer saccharomycete; via tube bacterial activation, triangle bottle bacterial, and 50L-1000L liquid ferment pot tube preparation to obtain the lactic acid sheet bacterial; via tube bacterial activation, triangle bottle bacterial, 50L-1000L liquid ferment pot preparation to obtain the bacillus licheniformis; then feeding the beer saccharomycete, lactic acid sheet bacterial, and bacillus licheniformis into large cultivate substrate; pooling, scraping material, and cultivating at constant temperature, drying, breaking, checking and packing. The invention can reduce the forage cost, improve quality, and reduce pollution.

Description

Biology enzyme additive of protein feed and production technology

Technical field: the present invention relates to biology enzyme additive of protein feed and the production technology used in a kind of aquaculture feedstuff industry.

Background technology: along with the raising of social development and people's living standard, livestock products play an important role aspect people's the quality of life improving, and with people ' s health and ecological environment substantial connection are arranged also.Since half a century, chemical classes regulator, antibiotic are at aspects such as people, animal health care, disease prevention and cure, all drawbacks come out gradually, and it is more and more serious, this has caused some pathogeny bacterium to develop immunity to drugs, antibiotic, regulator medicament residue also produce serious threat to livestock products consumer's health, and its consequence can not be imagined.Therefore, the use that is under an embargo gradually of chemobiotic and regulator, this requires people to seek new product to replace chemical classes antibiotic and regulator.

Summary of the invention: the invention provides a kind of technology uniqueness, improve the biology enzyme additive of protein feed and the production technology of aquatic animal quality.Described elder generation spreads cultivation to plant through female kind activation, triangular flask bacterial classification and curtain song and is prepared into the saccharomyces cerevisiae bacterial classification; Be prepared into lactic acid sheet bacterial through test tube strains activation, triangular flask bacterial classification and 50L and the production of hybrid seeds of 1000L liquid fermentation tank; Be prepared into the bacillus licheniformis bacterial classification through test tube strains activation, triangular flask bacterial classification and 50L and the production of hybrid seeds of 1000L liquid fermentation tank again; In proportion saccharomyces cerevisiae bacterial classification, lactic acid sheet bacterial and bacillus licheniformis bacterial classification are evenly inserted in the modulated aniseed culture medium that makes then, behind dress pond, the scraper constant temperature culture certain hour, drying and crushing is put in storage behind the cooperation test package.Because the present invention adopts high-tech green bio enzyme level Four sweat, by advanced person's prescription and special technology, make the biotic component of important function such as product is rich in animal growth and development, metabolism, digests and assimilates, immunological regulation, reduced feed cost, improved the quality of aquatic animal, reduced environmental pollution, and the green product that belongs to safety, health, environmental protection and economize on resources, thereby have good social benefit and economic benefit.

Description of drawings: Fig. 1 is a process chart of the present invention

The specific embodiment: shown in Figure 2 as Fig. 1, elder generation spreads cultivation to plant through female kind activation, triangular flask bacterial classification and curtain song and is prepared into the saccharomyces cerevisiae bacterial classification; Be prepared into lactic acid sheet bacterial through test tube strains activation, triangular flask bacterial classification and 50L and the production of hybrid seeds of 1000L liquid fermentation tank; Be prepared into the bacillus licheniformis bacterial classification through test tube strains activation, triangular flask bacterial classification and 50L and the production of hybrid seeds of 1000L liquid fermentation tank again; In proportion saccharomyces cerevisiae bacterial classification, lactic acid sheet bacterial and bacillus licheniformis bacterial classification are evenly inserted in the modulated aniseed culture medium that makes then, behind dress pond, the scraper constant temperature culture certain hour, drying and crushing is put in storage behind the cooperation test package.

As shown in Figure 1, the saccharomyces cerevisiae bacterial classification is to plant activation by mother, and its prescription is the beef extract-peptone agar medium; Its production technology is mother to be planted insert the sterile test tube culture medium under aseptic technique, and is after 30 ℃ of constant temperature are cultivated 72 hours, standby according to qualifications; Triangular flask bacterial classification prescription is bean cake powder 30g, wheat bran 40g, rice meal 10g, sucrose 5 and distilled water 1000ML; The test tube strains of will selecting the superior inserts aseptic triangular flask culture medium under aseptic technique, after 30 ℃ of constant temperature are cultivated 36 hours, standby according to qualifications; Curtain song kind of the prescription that spreads cultivation is bean cake powder 35%, wheat bran 45%, rice meal 10%, sucrose 1% and corn flour 9%; The triangular flask bacterial classification under aseptic technique, is inserted the bent culture medium of curtain of saccharification, sterilization in proportion, the shop curtain, after 30 ℃ of bacterial classification culturing room cultivated 36 hours, airing was standby.

As shown in Figure 1, lactic acid sheet bacterial, the test tube strains activation, its prescription is the beef extract-peptone agar medium; Its production technology is mother to be planted insert the sterile test tube culture medium under aseptic technique, and is after 37 ℃ of constant temperature are cultivated 24 hours, standby according to qualifications; Triangular flask bacterial classification prescription is bean cake powder 50g, wheat bran 30g, sodium chloride 2,5g, calcium carbonate 0,5g and distilled water 1000ML; The test tube strains of will selecting the superior inserts under aseptic technique in the aseptic triangular flask culture medium, by manufacturing parameter on the constant temperature shaking table 37 ℃ cultivate 24 hours after, excellent standby; The 50L and the production of hybrid seeds of 1000L liquid fermentation tank, 50L seeding tank prescription are bean cake powder 5%, wheat bran 3%, sodium chloride 0,25%, calcium carbonate 0,05%; 1000L fermentation tank prescription is bean cake powder 5%, wheat bran 2,5%, calcium carbonate 0,05%, glucose 2%, corn steep liquor 1% and corn flour 1,5%; Under aseptic technique, respectively by the manufacturing parameter inoculative proportion, bacterial classification is inserted modulated joining in the good fermentation tank culture liquid of sterilization successively, 37 ℃ of constant temperature constant speed were cultivated 36 hours, and are standby.

As shown in Figure 1, the bacillus licheniformis bacterial classification, the test tube strains activation, its prescription is the beef extract-peptone agar medium; Its production technology is mother to be planted insert the sterile test tube culture medium under aseptic technique, and is after 37 ℃ of constant temperature are cultivated 24 hours, standby according to qualifications; 1000ML triangular flask bacterial classification prescription is bean cake powder 80g, corn flour 10g, glucose 20g, distilled water 1000ML and PH7,2-6,5; The test tube strains of will selecting the superior inserts under aseptic technique in the aseptic triangular flask culture medium, and by manufacturing parameter on the constant temperature shaking table 40 ℃, 160r/m cultivated after 14 hours, and is standby; The 50L and the production of hybrid seeds of 1000L liquid fermentation tank, 50L fermentation tank prescription are bean cake powder 6%, corn flour 1,5%, glucose 2%, corn steep liquor 1% and PH6,5-7,5; 1000L fermentation tank prescription be bean cake powder 6%, corn flour 1,5%, glucose 2%, corn steep liquor 1%, hair extract 1,5% and and PH7,0; Under aseptic technique, respectively by the manufacturing parameter inoculative proportion, bacterial classification is inserted modulated joining in the good fermentation tank culture liquid of sterilization successively, 40 ℃ of constant temperature 170r/m constant speed were cultivated 36-48 hour, and are standby.

As shown in Figure 1, mixing dark pond level Four fermentating formula is bean cake powder 40%, corn flour 30%, blood meal 5%, big wing 10%, bacterial classification 5% and cotton dregs 10%; Press manufacturing technique requirent, in proportion saccharomyces cerevisiae bacterial classification, lactic acid sheet bacterial and bacillus licheniformis bacterial classification are evenly inserted in the modulated aniseed culture medium that makes, behind dress pond, the scraper constant temperature culture certain hour, airing is standby.

The mechanism of action of the present invention is because three big beneficial bacteriums have reached 6,500,000,000/gram in feed, to enter animal digestive system and be adsorbed in rapidly in the stomach cortex hair follicle, form dominant group, make pathogenic bacteria enter digestive system, do not have living space, to have reached the generation of prevention alimentary canal pathology.The body of keeping fit promotes fast growth of animal; Acid protease adds to 65 μ/grams again in feed, so can help animal that protein chain is cut into the polypeptide of short chain, be beneficial to animal and decompose free each required seed amino acid of animal completely from the peptide bond of protein, is absorbed by each body.Replenished energy enzymatic activity 350 μ/grams in the feed again, thus can help animal that crude fibre, starch, polysaccharide and pectin fully are degraded to monose or oligosaccharides, for animal provides heat energy, to reduce the waste of the energy in the feed.Additional phytate phosphorus reaches 6 μ/grams, can help animal that the bag of phytate phosphorus is thoroughly decomposed, and phosphatide is exposed in the alimentary canal of animal, is utilized by animal, and the interpolation of the calcium monohydrogen phosphate of replacement 40% alleviates the pollution of excreta to environment.

Claims

1, a kind of biology enzyme additive of protein feed and production technology is characterized in that earlier spreading cultivation and kind being prepared into the saccharomyces cerevisiae bacterial classification through female activation, triangular flask bacterial classification and curtain song of planting; Be prepared into lactic acid sheet bacterial through test tube strains activation, triangular flask bacterial classification and 50L and the production of hybrid seeds of 1000L liquid fermentation tank; Be prepared into the bacillus licheniformis bacterial classification through test tube strains activation, triangular flask bacterial classification and 50L and the production of hybrid seeds of 1000L liquid fermentation tank again; In proportion saccharomyces cerevisiae bacterial classification, lactic acid sheet bacterial and bacillus licheniformis bacterial classification are evenly inserted in the modulated aniseed culture medium that makes then, behind dress pond, the scraper constant temperature culture certain hour, drying and crushing is put in storage behind the cooperation test package.

2, biology enzyme additive of protein feed according to claim 1 and production technology is characterized in that the saccharomyces cerevisiae bacterial classification, are to plant activation by mother, and its prescription is the beef extract-peptone agar medium; Its production technology is mother to be planted insert the sterile test tube culture medium under aseptic technique, and is after 30 ℃ of constant temperature are cultivated 72 hours, standby according to qualifications; Triangular flask bacterial classification prescription is bean cake powder 30g, wheat bran 40g, rice meal 10g, sucrose 5g and distilled water 1000ML; The test tube strains of will selecting the superior inserts aseptic triangular flask culture medium under aseptic technique, after 30 ℃ of constant temperature are cultivated 36 hours, standby according to qualifications; Curtain song kind of the prescription that spreads cultivation is bean cake powder 35%, wheat bran 45%, rice meal 10%, sucrose 1% and corn flour 9%; The triangular flask bacterial classification under aseptic technique, is inserted the bent culture medium of curtain of saccharification, sterilization in proportion, the shop curtain, after 30 ℃ of bacterial classification culturing room cultivated 36 hours, airing was standby.

3, biology enzyme additive of protein feed according to claim 1 and production technology is characterized in that lactic acid sheet bacterial, the test tube strains activation, and its prescription is the beef extract-peptone agar medium; Its production technology is mother to be planted insert the sterile test tube culture medium under aseptic technique, and is after 37 ℃ of constant temperature are cultivated 24 hours, standby according to qualifications; Triangular flask bacterial classification prescription is bean cake powder 50g, wheat bran 30g, sodium chloride 2,5g, calcium carbonate 0,5g and distilled water 1000ML; The test tube strains of will selecting the superior inserts under aseptic technique in the aseptic triangular flask culture medium, by manufacturing parameter on the constant temperature shaking table 37 ℃ cultivate 24 hours after, standby according to qualifications; The 50L and the production of hybrid seeds of 1000L liquid fermentation tank, 50L seeding tank prescription are bean cake powder 5%, wheat bran 3%, sodium chloride 0,25%, calcium carbonate 0,05%; 1000L fermentation tank prescription is bean cake powder 5%, wheat bran 2,5%, calcium carbonate 0,05%, glucose 2%, corn steep liquor 1% and corn flour 1,5%; Under aseptic technique, respectively by the manufacturing parameter inoculative proportion, bacterial classification is inserted modulated joining in the good fermentation tank culture liquid of sterilization successively, 37 ℃ of constant temperature constant speed were cultivated 36 hours, and are standby.

4, biology enzyme additive of protein feed according to claim 1 and production technology, its feature bacillus licheniformis bacterial classification, the test tube strains activation, its prescription is the beef extract-peptone agar medium; Its production technology is mother to be planted insert the sterile test tube culture medium under aseptic technique, and is after 37 ℃ of constant temperature are cultivated 24 hours, standby according to qualifications; 1000ML triangular flask bacterial classification prescription is bean cake powder 80g, corn flour 10g, glucose 20g, distilled water 1000ML and PH7,2-6,5; The test tube strains of will selecting the superior inserts under aseptic technique in the aseptic triangular flask culture medium, and by manufacturing parameter on the constant temperature shaking table 40 ℃, 160r/m cultivated after 14 hours, and is standby; The 50L and the production of hybrid seeds of 1000L liquid fermentation tank, 50L fermentation tank prescription are bean cake powder 6%, corn flour 1,5%, glucose 2%, corn steep liquor 1% and PH6,5-7,5; 1000L fermentation tank prescription be bean cake powder 6%, corn flour 1,5%, glucose 2%, corn steep liquor 1%, hair extract 1,5% and and PH7,0; Under aseptic technique, respectively by the manufacturing parameter inoculative proportion, bacterial classification is inserted modulated joining in the good fermentation tank culture liquid of sterilization successively, 40 ℃ of constant temperature 170r/m constant speed were cultivated 36-48 hour, and are standby.

5, biology enzyme additive of protein feed according to claim 1 and production technology, it is characterized in that mixing dark pond level Four fermentating formula is bean cake powder 40%, corn flour 30%, blood meal 5%, big wing 10%, bacterial classification 5% and cotton dregs 10%; Press manufacturing technique requirent, in proportion saccharomyces cerevisiae bacterial classification, lactic acid sheet bacterial and bacillus licheniformis bacterial classification are evenly inserted in the modulated aniseed culture medium that makes, behind dress pond, the scraper constant temperature culture certain hour, airing is standby.