CN1894410A - 谷类第4组主要致敏原的dna序列和重组制品 - Google Patents
谷类第4组主要致敏原的dna序列和重组制品 Download PDFInfo
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Abstract
本发明涉及到来自谷物类的第4组主要致敏原的DNA序列的提供，本发明也包括具有降低过敏作用的片段、部分序列的新组合及含有点突变的突变体。重组的DNA分子和其衍生而来的多肽、片段、部分序列的新组合和变异体可用于花粉过敏性疾病的治疗。由重组方法制备的蛋白可用于花粉过敏的体外和体内诊断。
Description
发明背景
本发明涉及到来自谷类(小麦族(Triticeae))的第4组主要致敏原的DNA序列的提供，本发明也包括具有降低过敏作用的片段、部分序列的新组合及含有点突变的突变体。重组的DNA分子和其衍生而来的多肽、片段、部分序列的新组合和变异体可以被用于花粉过敏性疾病的治疗。由重组方法制备的蛋白可以用于花粉过敏的体外和体内诊断。
I型过敏反应是全球性的，在工业国家中有多至20％的人口患有如过敏性鼻炎、结膜炎或支气管哮喘。这些过敏由从各种来源如植物花粉、螨类、猫和狗释放到空气中的致敏原(空气致敏原)引起，多至40％的I型过敏患者有对禾本花粉致敏原的特异性IgE反应，对谷类花粉致敏原尤甚(Freidhoff et al.，1986，J.Allergy Clin.Immunol.78，1190-2001)，在谷类花粉致敏原中，又以黑麦致敏原为最重要。
触发I型过敏反应的物质为蛋白、糖蛋白或多肽，通过粘膜被摄取后，在已被致敏的个体体内这些致敏原与结合于肥大细胞表面的I，分子反应，如果两个IgE分子通过致敏原相互交叉连接，即会导致效应细胞释放介质(如组胺、前列腺素)和细胞因子，并引起相应的临床症状。
根据各种致敏原与过敏患者的IgE反应的相对频率，将致敏原区分为主要致敏原和次要致敏原。
禾本科(Poaceae)来源的各种花粉被划分为不同组，各组之间相互同源。
特别值得一提的是主要致敏原第4组中的分子相互间具有很强的交叉免疫反应，对鼠单克隆抗体和人IgE抗体均如此(Fahlbusch et1993 Clin.Exp.Allergy 23：51-60；Leduc-Brodard et 1996，J.AllergyClin.Immunol.98：1065-1072；Su et 1996，J.Allergy Clin.Immunol.97：210；et 1998，Clin.Exp.Allergy 28：799-807；Gavrovic-et 2000，10(6)：361-367；Stumvoll et al.2002，Biol.Chem.383：1383-1396；Grote et2002，Chem.383：1441-1445；Andersson und Lidholm，2003，Arch.Allergy Immunol.130：87-107；Mari，Clin.Exp.Allergy，33(1)：43-51)。
迄今为止，尚没有一个第4组主要致敏原的完整DNA序列被弄清楚。
迄今第4组致敏原中的鸭茅(Dactylus glomerata)致敏原只可能通过酶消化获得多肽并进行序列测定：DIYNYMEPYVSK(SEQ ID NO 13)，VDPTDYFGNEQ(SEQ ID NO 14)，ARTAWVDSGAQLGELSY(SEQ ID NO 15)和GVLFNIQYVNYWFAP(SEQ NO 16，Leduc-Brodard et J.AllergyClin.Immunol.98：1065-1072)。
通过蛋白水解获得肽并测定了序列的还有致敏原第4组中的亚热带狗牙根(Cynodon dactylon)：KTVKPLYIITP(SEQ ID NO 17)KQVERDFLTSLTKDIPQLYLKS(SEQ ID NO 18)TVKPLYIITPITAAMI(SEQ ID NO 19)LRKYGTAADNVIDAKVVDAQGRLL(SEQ ID NO 20)KWQTVAPALPDPNM(SEQ ID NO 21)VTWIESVPYIPMGDK(SEQ ID NO 22)GTVRDLLXRTSNIKAFGKY(SEQ ID NO 23)TSNIKAFGKYKSDYVLEPIPKKS(SEQ ID NO 24)YRDLDLGVNQVVG(SEQ ID NO 25)SATPPTHRSGVLFNI(SEQ ID NO 26)和AAAALPTQVTRDIYAFMTPYVSKNPRQAYVNYRDLD(SEQ IDNO 17，Liaw et 2001，Biochem.Biophys.Research Communication 280：738-743)).
对于黑麦草(Lolium perenne)，作为第4组基本致敏原的具有以下序列的肽片段已有描述：FLEPVLGLIFPAGV(SEQ ID NO 28)和GLIEFPAGV(SEQ ID NO 29 Jaggi et 1989，Int.Arch.342-348)。
尚未发表的来自梯牧草(Phleum pratense)的PhI p 4序列(SEQ IDNO 11)，作为第一个第4组致敏原序列，本专利申请的发明者已在国际申请WO 04/000881中对其进行了阐述。
迄今为止还不知道来自谷类(小麦族(Triceae))的第4组致敏原的任何序列。
因此，本发明的目的是要提供来自谷类的第4组致敏原的DNA序列，特别是来自黑麦(Secale cerale)的Sec c 4(SEQ ID NO 1、3)、来自大麦(Hordeum vulgare)的Hor v 4(SEQ ID NO 5)和来自小麦(Triticum aestivum)的Tri a 4(SEQ ID NO 7、9)以及相应的重组DNA分子，以这些重组DNA分子为基础，致敏原能以无修饰的或修饰过的蛋白形式表达进而得到，用于有意义的药物开发。PhI p 4序列(SEQID NO 11)是本发明的起始点。
本发明的序列表
与SEQ ID NO 1-10对应的成熟致敏原的DNA和蛋白序列前面带有信号序列，其编码区终止于DNA序列上的TGA或TAG终止密码子。
-Sec c 4DNA序列(a)同工型Sec c 4.01(SEQ ID NO 1)，(b)同工型Secc 4.02(SEQ ID NO 3)-来源于与SEQ ID NO 1和3相应的DNA序列的蛋白序列(SEQ IDNO 2、4)
-Hor v 4的DNA序列(SEQ ID NO 5)
-来源于与SEQ ID NO 5相应的DNA序列的蛋白序列(SEQ ID NO 6)
-Tria 4 DNA序列(a)同工型Tria 4.01(SEQ ID NO 7)，(b)同工型Tria 4.02(SEQ ID NO 9)
-来源于与SEQ ID NO 7和9相应的DNA序列的蛋白序列(SEQ IDNO 8、10)
-对应于WO 04/000881中的SEQ ID NO 5的PhI p 4DNA序列(SEQID NO 11)
-对应于WO 04/000881中的SEQ ID NO 6的PhI p 4蛋白序列(SEQID NO 12)
发明描述
本发明目前第一次提供谷类花粉主要致敏原Sec c 4、Hor v 4和Tria 4的DNA序列，对应的序列号为SEQ ID NO1、3、5、7和9。本发明因此涉及到选自对应于SEQ ID NO1、3、5、7和9的核酸序列的DNA分子。
本发明进一步涉及到与本发明的DNA序列同源的序列和来源于其他禾本科(poaceae)的第4组致敏原的相应的DNA分子，这类植物有黑麦草(Lolium perenne)、鸭茅(Dactylis glomerata)、草地早熟禾(Poapratensis)、狗牙根(Cynodon dactylon)和绒毛草(Holcus Lanatus)，由于存在同源性，它们能在严格条件下与本发明的DNA序列杂交或与本发明的致敏原发生交叉免疫反应。
本发明也包括具有降低过敏反应作用的片段、部分序列的新组合和含有点突变的突变体。
本发明因此进一步涉及到编码来自禾本科(poaoaceae)的第4组致敏原的免疫调节、T细胞反应片段的对应的部分序列、部分序列的组合，或含有取代、删除或添加的突变体。
随着对自然发生的致敏原DNA序列的了解，目前，已经有可能制备能够用于过敏性疾病的诊断和治疗的这些致敏原的重组蛋白(Scheiner and Kraft，1995，Allergy 50：384-391)。
经典的有效治疗过敏的方法为特异性免疫治疗或脱敏治疗(Fiebig，1995，J.4(6)：336-339，Bousquet et 1998，J.Alllergy Clin.Immunol.102(4)：558-562)。在此方法中，自然致敏原的提取物以不断提高的剂量给予病人皮下注射。然而这种方法有引起过敏，甚至过敏性休克的危险，为了最大限度地降低这种危险，使用了新型的类致敏原制剂，它们是经过化学修饰的致敏原提取物，其IgE反应性已明显降低，但T细胞反应与未处理的提取物相同(Fiebig，1995，Allergo J.4(7)：377-382)。
用重组方法制备的致敏原使更有实质意义的优化治疗成为了可能。通过重组方法获得的且任选性地与病人个体的过敏模式匹配的高纯度的致敏原“鸡尾酒”可以取代自然致敏原来源的提取物，因为除了各种致敏原外，它们含有相对大量的具有免疫原性但无过敏性的二级蛋白。
用表达产物引起可靠的脱敏反应的现实前景因使用经过特异性突变删除了IgE表位，但不损害治疗作用所必需的T细胞表位的重组致敏原而产生(Schramm等，1999，J.Immunol.162：2406-2414)。
治疗过敏患者体内TH细胞失衡的另一个可能方法是免疫治疗性DNA疫苗接种，它涉及到用编码相关致敏原的表达性DNA进行治疗。通过给啮齿动物注射致敏原编码DNA已得到致敏原特异性免疫应答影响的初步实验证据(Schramm et 1999，J.Immunol.162：2406-2414)。
本发明因此涉及到以上或以下描述的作为药物的DNA分子和作为药物的相应的重组表达载体。
通过重组方法制备的相应的蛋白可以被用于花粉过敏的治疗及体外及体内诊断。
为了制备重组致敏原，克隆的核酸被连接到表达载体上，该构建体在一个适合的宿主生物体内表达，经过生物化学纯化，即可通过已建立的方法用重组致敏原检测IgE抗体。
本发明进一步涉及到含有以上或以下描述的功能性连接到表达控制序列的DNA分子的重组表达载体和被所述DNA分子或所述表达载体转化的宿主生物。
本发明还涉及到至少一种以上描述的DNA分子或至少一种以上描述的表达载体在制备用于禾本科(poaceae)第4组致敏原，优选是小麦族(Triticeae)，尤其是Sec c 4、Hor v 4、Tri a 4参与触发的过敏病人的免疫治疗性DNA药物和/或预防这些过敏的药物中的用途。
正如已经陈述的那样，本发明可以作为重组致敏原或含核酸制剂的基本成分用于特异性免疫治疗，许多种可能在这里呈现，一方面，制剂的组成物可以是一级结构无改变的蛋白；另一方面，根据本发明，可以通过整体特异性删除分子上的IgE表位或产生编码T细胞表位的单一片段产生低致敏(类致敏原)形式来进行治疗以避免不必要的副作用；最后，核酸本身如果被连接到真核表达载体而制成制剂直接使用，能够治疗性地调节过敏免疫状态。
本发明进一步涉及到由以上描述的一个或多个DNA分子编码的多肽，特别是它们作为药物的效用。
它们是具有与SEQ ID NO 2、4、6、8或10一致的氨基酸序列的蛋白，特别值得一提的是它们是成熟蛋白(无信号序列成分)，起始于第23位氨基酸(SEQ ID NO 2、4和6)和第22位氨基酸(SEQ ID NO 8和10)。本发明进一步涉及到含有这些氨基酸序列或这些序列的组成部分的蛋白。
本发明因此也涉及到通过培养宿主生物制备这样的多肽和从培养物中分离相应多肽的方法。
本发明同样涉及到至少一种上述多肽在制备用于诊断和/或治疗禾本科(poaceae)，特别是小麦族(Triticeae)的第4组致敏原，尤其是Secc4、Hor v 4、Tri a 4参与触发的过敏反应的药物和预防这些致敏原的药物中的用途。
在确定本发明的蛋白和DNA序列时，应用了以下步骤：
来自黑麦的Sec c 4
1.从PhI p 4 DNA序列(SEQ ID NO 12，WO04/000881)入手，合成来自PhI p 4序列的特异性引物(表1)，通过PCR用引物#87和#83从黑麦花粉DNA上获得5个克隆，扩增出的对应这些克隆的Sec c 4基因片段1编码与PhI p 4(SEQ ID NO 12)上第68-401位氨基酸序列对应的多肽。
2.用Sec c 4的部分序列在EST数据库进行了检索，在专门的黑麦EST数据库中没有发现与之同源的序列，然而，发现单个的、同源的、非重叠的EST片段在专门的大麦和小麦的EST数据中存在。单个的EST片段延伸至对应基因的5’-UTR区，其他延伸至3’-UTR区(UTR＝非翻译区)。
3.然而，无法从EST数据库中的EST序列拼接出小麦和大麦的完整的第4组基因，因为这些序列不重叠，而且不知道第4组基因的同源序列。但是，参考PhI p 4序列(SEQ ID NO 11)和在步骤1获得的Sec c 4片段可以定位这些EST序列，这些序列可用作制备PCR引物的模板。
4.有了通过这种途径制备的引物#195和#189的帮助，用PCR得到3个克隆，引物#195来自大麦EST序列，引物#189为PhI p 4特异性引物，并与PhI p 4的终止密码子和PhI p 4的C末端的10个氨基酸序列重叠。通过这种途径扩增的Sec c 4基因片段2编码一个起始于信号序列，终止于与PhI p 4对应的490位的多肽。该多肽覆盖Sec c 4的N末端。
5a.用引物#195和#202通过PCR获得了另外3个克隆。这两个引物均来自大麦的EST序列，扩增的Sec c 4基因片段3编码起始于Sec c 4的信号序列，终止于Sec c 4的C末端的氨基酸。由此成熟Secc 4的完整序列以确定的序列被呈现出来。
以下5b和5c两个步骤用于对步骤5a获得的结果的“双保险”确定。5b.通过PCR用引物#195和#203获得了另外3个克隆，引物#195来自大麦的EST序列，引物#203来自小麦EST序列。该扩增的Sec c 4基因编码起始于Sec c 4的信号序列，终止于Sec c 4的C末端的对应氨基酸，由此成熟Sec c 4的完整序列以确定的序列被呈现出来。
5c.用引物#195和#198通过PCR获得了另外1个克隆，(alsoprimer#198)，该扩增的Sec c 4基因编码起始于Sec c 4的信号序列，终止于Sec c 4的C末端的对应氨基酸，由此成熟Sec c 4的完整序列以确定的序列被呈现出来。
发现有Sec c 4.01和Sec c 4.02两种同工型，成熟致敏原起始于对应于SEQ ID NO 2、4和6序列的第23位氨基酸。来自大麦的Hor v 4
有通过以上描述获得的Sec c 4序列的帮助，发现同源的EST片段在大麦(Hordeum vulgare)的EST数据库中存在。这些片段重叠、但从中得不出完整基因。参考已发现的EST片段，有可能设计出Horv 4特异性引物，用于从染色体DNA中扩增Hor v 4基因。
总共分析了15个克隆
4个克隆用引物#195和#198通过PCR获得。
4个克隆用引物#195和#202通过PCR获得。
3个克隆用引物#194和#198通过PCR获得。
4个克隆用引物#194和#202通过PCR获得。
其衍生的蛋白序列起始于Hor v 4的信号序列并延伸至该蛋白的C末端(从SEQ ID NO 6的第23位氨基酸起)
来自小麦的Tri a 4
有通过以上描述获得的Sec c 4序列的帮助，发现同源的EST片段在小麦(Triticum aestivum)的EST数据库中存在。这些片段重叠，但从中得不出完整的基因。参考已发现的EST片段，有可能设计出Tria 4特异性引物#199、#203、#204和#206，用于从染色体DNA中扩增Tri a 4基因。
总共分析了13个克隆
4个克隆用引物#204和#203通过PCR获得。
4个克隆用引物#204和#199通过PCR获得。
4个克隆用引物#206和#203通过PCR获得。
4个克隆用引物#206和#199通过PCR获得。
其衍生的蛋白序列起始于Tri a 4的信号序列并延伸至该蛋白的C末端。发现有Tri a 4.01(从SEQ ID NO 8的第22位氨基酸起)和Tri a4.02(从SEQ ID NO 10的第22位氨基酸起)两个变异体。
为了制备本发明的重组致敏原，对应于SEQ ID NO 1、3、5、7和9的DNA序列被重组进表达载体(例如pProEx、pSE 380)，应用了E.coli优化密码子来编码从该蛋白序列测定所知的N端氨基酸。
在转化进E.coli后，通过各种分离技术对本发明的重组致敏原进行表达和纯化，得到的蛋白质进行重新折叠。两种致敏原均可用于禾本花粉过敏的高特异性诊断，诊断可以是在体外通过检测特异性抗体(IgE、IgG1-4、IgA)并与携带IgE效应细胞(如血液中的嗜碱性白细胞)反应来进行，也可以是在体内通过皮试反应和在反应器官进行诱发来进行。
通过致敏原特异性刺激T淋巴细胞引起的增殖和细胞因子合成可以检测到禾本花粉过敏患者的T淋巴细胞与本发明的致敏原的反应，细胞可以是新鲜制备的血淋巴细胞中的T细胞，也可以是已经建立的n Sec c 4、nHor v 4或nTri a 4反应性T细胞系和克隆。
对编码半胱氨酸的三联子通过特异性点突变技术进行修饰，使其编码其他氨基酸，优选丝氨酸，制备出了单个半胱氨酸被替代的变异体和那些各种组合的两个半胱氨酸残基或所有半胱氨酸被修饰的变异体，这些半胱氨酸点突变体表达的蛋白与过敏患者的IgE抗体的反应被大大降低或与IgE抗体无反应，但能与这些病人的T淋巴细胞发生反应。
本发明因此涉及到以上或以下描述的DNA分子，这些DNA分子对应的多肽中的一个、多个或所有半胱氨酸残基通过特异性点突变技术被另一个氨基酸所取代。
含有T细胞表位和有低致敏性点突变(例如半胱氨酸取代)的多肽对应的低致敏性片段的免疫调节活性可以通过它们与禾本花粉过敏患者的T细胞进行反应来检测。
这样的低致敏片段或半胱氨酸点突变体可以用作过敏患者的脱敏制剂，因为它们与T细胞的反应效力不变，但由于与IgE的反应性降低或完全缺失所以它们引起的IgE介导的副作用会很低。
如果本发明的编码低致敏性致敏原变异体的核酸或本发明的未经修饰的DNA分子被连接到人体表达载体，这些构建体同样可用作免疫治疗(DNA疫苗接种)制剂。
最后，本发明涉及到用于禾本科(poaceae)，特别是小麦族(Triticeae)的第4组致敏原，尤其是Sec c 4、Hor v 4、Tri a 4参与触发的过敏反应的免疫治疗或预防这些过敏反应的药用组合物，该药用组合物包括至少一种上述DNA分子或至少一种上述表达载体和可选的其他活性成分和/或佐剂。
本发明的另一组适用于所述过敏反应的诊断和/或治疗的药用组合物包含至少一种上述多肽，而不是DNA。
本发明意义上的药用组合物包含作为活性成分的本发明的多肽或表达载体和/或它们各自的药学上适用的衍生物，包括以各种比率混合的它们的混合物。本发明的活性成分可以与至少一种固体、液体和/或半液体赋形剂或佐剂做成合适的剂量形式，并可选择性地与一种或多种其他活性成分联用。
特别合适的佐剂为免疫刺激DNA或含有CpG基序的寡核苷酸。
这些组合物可以用作人类或兽类医疗方面的治疗药物或诊断试剂。合适的赋形剂为适合于肠外给药且对本发明的活性成分的功效没有负面影响的有机或无机物质。适合于肠外使用的，具体有溶液剂，优选油性或水性溶液剂，此外有混悬剂、乳剂或植入剂。本发明的活性成分也可以被冷冻干燥，形成的冻干物用于制备注射制剂。所指组合物可以是灭菌的和/或含有佐剂，如防腐剂、稳定剂和/或润湿剂、乳化剂、调节渗透压的盐、缓冲物质和/或多种其他活性成分。
此外，可以通过相应配制本发明活性成分，例如将其吸附于氢氧化铝而获得缓释制剂。
本发明还作为病人特异性过敏谱的致敏原触发成分鉴别试验的一部分用于改善体外诊断，本发明同样用于禾本花粉过敏的特异性免疫治疗的显著改善制剂的制备。
表1.所用引物
a)Sec c 4
| 引物编号 | SEQ ID NO | 序列 |
| #0083 | 30 | GGCTCCCGGGGCGAACCAGTAG |
| #0087 | 31 | ACCAACGCCTCCCACATCCAGTC |
| #0189 | 32 | GATAAGCTTCTCGAGTGATTAGTACTTTTTGATCAGCGGCGGGATGCTC |
| #0195 | 33 | GCTCTCGATCGGCTACAATGGCG |
| #0198 | 34 | CACGCACTACAAATCTCCATGCAAG |
| #0202 | 35 | CATGCTTGATCCTTATTCTACTAGTTGGGC |
| #0203 | 36 | TACGCACGATCCTTATTCTACTAGTTGGGC |
b)Hor V 4
| 引物编号 | SEQ ID NO | 序列 |
| #0194 | 37 | GCCTTGTCCTGCCACCACGCCGCCGCCACC |
| #0195 | 38 | GCTCTCGATCGGCTACAATGGCG |
| #0198 | 39 | CACGCACTACAAATCTCCATGCAAG |
| #0202 | 40 | CATGCTTGATCCTTATTCTACTAGTTGGGC |
c)Tri a 4
| 引物编号 | SEQ ID NO | 序列 |
| #0199 | 41 | CACGCACTAAATCTCCATGCAAG |
| #0203 | 42 | TACGCACGATCCTTATTCTACTAGTTGGGC |
| #0204 | 43 | AAGCTCTATCGCCTACAATGGCG |
| #0206 | 44 | GGTGCTCCTCTTCTGCGCCTTGTCC |
序列表
<110>默克专利股份公司
<120>谷类第4组主要致敏原的DNA序列和重组制品
<130>P 03/239
<140>DE 10359351.9
<141>2003-12-16
<160>44
<170>PatentIn version 3.1
<210>1
<211>1603
<212>DNA
<213>黑麦(Secale cereale)
<220>
<221>stop_codon
<222>(1555)..(1557)
<223>
<220>
<221>signal_sequence_DNA
<222>(1)..(66)
<223>
<220>
<221>signal_sequence_PROT
<222>(1)..(22)
<223>
<220>
<221>CDS
<222>(1)..(1557)
<223>
<400>1
aac tat agg gcc ttc gcg ctg gcg ctc ctc ttc tgc gcc ttg tcc tgc 48
Asn Tyr Arg Ala Phe Ala Leu Ala Leu Leu Phe Cys Ala Leu Ser Cys
1 5 10 15
caa gcc gcc gcg gcc gcc tac gcg ccc gtg cct gcc aag gcg gac ttc 96
Gln Ala Ala Ala Ala Ala Tyr Ala Pro Val Pro Ala Lys Ala Asp Phe
20 25 30
ctc gga tgc ctc atg aag gag ata ccg gcc cgc ctc ctc tac gcc aag 144
Leu Gly Cys Leu Met Lys Glu Ile Pro Ala Arg Leu Leu Tyr Ala Lys
35 40 45
agc tcg cct gac tac ccc acc gtg ctg gcg cag acc atc agg aac tcg 192
Ser Ser Pro Asp Tyr Pro Thr Val Leu Ala Gln Thr Ile Arg Asn Ser
50 55 60
cgg tgg tcg tcg ccg cag aac gtg aag ccg atctac atc atc acc ccc 240
Arg Trp Ser Ser Pro Gln Asn Val Lys Pro Ile Tyr Ile Ile Thr Pro
65 70 75 80
acc aac gcc tcg cac atc cag tcc gcg gtg gtg tgc ggc cgc cgg cac 288
Thr Asn Ala Ser His Ile Gln Ser Ala Val Val Cys Gly Arg Arg His
85 90 95
ggc atc cgc ctc cgc gtg cgg agc ggc ggc cac gactac gag ggc ctg 336
Gly Ile Arg Leu Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu
100 105 110
tcg tac cgg tct gag aaa ccc gag acg ttc gcc gtc gtc gac ctc aac 384
Ser Tyr Arg Ser Glu Lys Pro Glu Thr Phe Ala Val Val Asp Leu Asn
115 120 125
aag atg cgg gca gtg tcg gtc gac ggc tac gcc cgc acg gcg tgg gtc 432
Lys Met Arg Ala Val Ser Val Asp Gly Tyr Ala Arg Thr Ala Trp Val
130 135 140
gaa tcc ggc gcg cag ctc ggc gag ctc tac tac gcg atc gcc aag aac 480
Glu Ser Gly Ala Gln Leu Gly Glu Leu Tyr Tyr Ala Ile Ala Lys Asn
145 150 155 160
agc ccc gtg ctc gcg ttc ccg gct ggc gtc tgc ccgtcc atc ggc gtc 528
Ser Pro Val Leu Ala Phe Pro Ala Gly Val Cys Pro Ser Ile Gly Val
l65 l70 l75
ggc ggc aac ttc gca ggc ggc ggc ttt ggc atg ctg ctg cgc aag tac 576
Gly Gly Asn Phe Ala Gly Gly Gly Phe Gly Met Leu Leu Arg Lys Tyr
180 185 190
ggc atc gcc gct gag aac gtc atc gac gtc aag gtg gtc gac ccc aac 624
Gly Ile Ala Ala Glu Asn Val Ile Asp Val Lys Val Val Asp Pro Asn
195 200 205
ggc aag ctg ctc gac aag agc tcc atg agc gcg gac cac ttc tgg gcc 672
Gly Lys Leu Leu Asp Lys Ser Ser Met Ser Ala Asp His Phe Trp Ala
210 215 220
gtt agg ggc ggc ggc gga gag agc ttt ggc atc gtc gtc tcg tgg cag 720
Val Arg Gly Gly Gly Gly Glu Ser Phe Gly Ile Val Val Ser Trp Gln
225 230 235 240
gtg aag ctc ctg ccg gtg cct ccc acc gtg acc gtg ctc aag atc ccc 768
Val Lys Leu Leu Pro Val Pro Pro Thr Val Thr Val Leu Lys Ile Pro
245 250 255
aag acg gtg caa gaa ggc gcc ata gac ctc gtc aac aag tgg cag ctg 816
Lys Thr Val Gln Glu Gly Ala Ile Asp Leu Val Asn Lys Trp Gln Leu
260 265 270
gtc ggg ccg gca ctt ccc ggc gac ctc atg atc cgc atc atc ctt gcc 864
Val Gly Pro Ala Leu Pro Gly Asp Leu Met Ile Arg Ile Ile Leu Ala
275 280 285
ggg aac agc gcg acg ttc gag gcc atg tac ctg ggc acc tgc agt acc 912
Gly Asn Ser Ala Thr Phe Glu Ala Met Tyr Leu Gly Thr Cys Ser Thr
290 295 300
ctg acg ccg ctg atg agc agc aaa ttc ccc gag ctt ggc atg aac ccc 960
Leu Thr Pro Leu Met Ser Ser Lys Phe Pro Glu Leu Gly Met Asn Pro
305 310 315 320
tcg cac tgc aac gag atg tcc tgg atc aag tcc atc ccc ttc atc cac 1008
Ser His Cys Asn Glu Met Ser Trp Ile Lys SerIle Pro Phe Ile His
325 330 335
ctc ggc aag cag aac ctc gac gac ctc ctc aac cgg aac aac acc ttc 1056
Leu Gly Lys Gln Asn Leu Asp Asp Leu Leu Asn Arg Asn Asn Thr Phe
340 345 350
aaa cca ttc gcc gaa tac aag tcg gac tac gtg tac cag ccc ttc ccc 1104
Lys Pro Phe Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln Pro Phe Pro
355 360 365
aag ccc gtg tgg gag cag atc ttc ggc tgg ctt gtg aag ccc ggc gcg 1152
Lys Pro Val Trp Glu Gln Ile Phe Gly Trp Leu Val Lys Pro Gly Ala
370 375 380
ggg atc atg atc atg gac ccc tat ggc gcc acc atc agc gct acc ccc 1200
Gly Ile Met Ile Met Asp Pro Tyr Gly Ala Thr Ile Ser Ala Thr Pro
385 390 395 400
gaa gcg gcg acg ccg ttc cct cac cgc cag ggc gtc ctc ttc aac atc 1248
Glu Ala Ala Thr Pro Phe Pro His Arg Gln Gly Val Leu Phe Asn Ile
405 410 415
cag tac gtc aac tac tgg ttc gct gag tca gcc ggc gcg gcg ccg ctg 1296
Gln Tyr Val Asn Tyr Trp Phe Ala Glu Ser Ala Gly Ala Ala Pro Leu
420 425 430
cag tgg agc aag gac ata tac aag ttc atg gag ccg tac gtg ago aaa 1344
Gln Trp Ser Lys Asp Ile Tyr Lys Phe Met Glu Pro Tyr Val Ser Lys
435 440 445
aat ccc agg cag gcg tat gcc aac tac agg gac atc gac ctt ggc agg 1392
Asn Pro Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp Leu Gly Arg
450 455 460
aat gag gtg gtg aac gac atc tcc acc tac agc agc ggc aaa gtg tgg 1440
Asn Glu Val Val Asn Asp Ile Ser Thr Tyr Ser Ser Gly Lys Val Trp
465 470 475 480
ggt gag aag tac ttc aag ggc aac ttc caa agg ctc gcc att acc aag 1488
Gly Glu Lys Tyr Phe Lys Gly Asn Phe Gln Arg Leu Ala Ile Thr Lys
485 490 495
ggc aag gtg gat cct cag gac tac ttc agg aac gag cag agc atc ccg 1536
Gly Lys Val Asp Pro Gln Asp Tyr Phe Arg Asn Glu Gln Ser Ile Pro
500 505 510
cca ctg gtc gag aag tac tga tcgaggacct tgcatggaaa tttagtgcgt 1587
Pro Leu Val Glu Lys Tyr
515
ggttggcgtt tcacat 1603
<210>2
<211>518
<212>PRT
<213>黑麦(Secale cereale)
<400>2
Asn Tyr Arg Ala Phe Ala Leu Ala Leu Leu Phe Cys Ala Leu Ser Cys
1 5 10 15
Gln Ala Ala Ala Ala Ala Tyr gla Pro Val Pro Ala Lys Ala Asp Phe
20 25 30
Leu Gly Cys Leu Met Lys Glu Ile Pro Ala Arg Leu Leu Tyr Ala Lys
35 40 45
Ser Ser Pro Asp Tyr Pro Thr Val Leu Ala Gln Thr Ile Arg Asn Ser
50 55 60
Arg Trp Ser Ser Pro Gln Asn Val Lys Pro Ile Tyr Ile Ile Thr Pro
65 70 75 80
Thr Asn Ala Ser His Ile Gln Ser Ala Val Val Cys Gly Arg Arg His
85 90 95
Gly Ile Arg Leu Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu
100 105 110
Ser Tyr Arg Ser Glu Lys Pro Glu Thr Phe Ala Val Val Asp Leu Asn
115 120 125
Lys Met Arg Ala Val Ser Val Asp Gly Tyr Ala Arg Thr Ala Trp Val
130 135 140
Glu Ser Gly Ala Gln Leu Gly Glu Leu Tyr Tyr Ala Ile Ala Lys Asn
145 150 155 160
Ser Pro Val Leu Ala Phe Pro Ala Gly Val Cys Pro Ser Ile Gly Val
165 170 175
Gly Gly Asn Phe Ala Gly Gly Gly Phe Gly Met Leu Leu Arg Lys Tyr
180 185 190
Gly Ile Ala Ala Glu Asn Val Ile Asp Val Lys Val Val Asp Pro Asn
195 200 205
Gly Lys Leu Leu Asp Lys Ser Ser Met Ser Ala Asp His Phe Trp Ala
210 215 220
Val Arg Gly Gly Gly Gly Glu Ser Phe Gly Ile Val Val Ser Trp Gln
225 230 235 240
Val Lys Leu Leu Pro Val Pro Pro Thr Val Thr Val Leu Lys Ile Pro
245 250 255
Lys Thr Val Gln Glu Gly Ala Ile Asp Leu Val Asn Lys Trp Gln Leu
260 265 270
Val Gly Pro Ala Leu Pro Gly Asp Leu Met Ile Arg Ile Ile Leu Ala
275 280 285
Gly Asn Ser Ala Thr Phe Glu Ala Met Tyr Leu Gly Thr Cys Ser Thr
290 295 300
Leu Thr Pro Leu Met Ser Ser Lys Phe Pro Glu Leu Gly Met Asn Pro
305 310 315 320
Ser His Cys Asn Glu Met Ser Trp Ile Lys Ser Ile Pro Phe Ile His
325 330 335
Leu Gly Lys Gln Asn Leu Asp Asp Leu Leu Asn Arg Asn Asn Thr Phe
340 345 350
Lys Pro Phe Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln Pro Phe Pro
355 360 365
Lys Pro Val Trp Glu Gln Ile Phe Gly Trp Leu Val Lys Pro Gly Ala
370 375 380
Gly Ile Met Ile Met Asp Pro Tyr Gly Ala Thr Ile Ser Ala Thr Pro
385 390 395 400
Glu Ala Ala Thr Pro Phe Pro His Arg Gln Gly Val Leu Phe Asn Ile
405 410 415
Gln Tyr Val Asn Tyr Trp Phe Ala Glu Ser Ala Gly Ala Ala Pro Leu
420 425 430
Gln Trp Ser Lys Asp Ile Tyr Lys Phe Met Glu Pro Tyr Val Ser Lys
435 440 445
Asn Pro Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp Leu Gly Arg
450 455 460
Asn Glu Val Val Asn Asp Ile Ser Thr Tyr Ser Ser Gly Lys Val Trp
465 470 475 480
Gly Glu Lys Tyr Phe Lys Gly Asn Phe Gln Arg Leu Ala Ile Thr Lys
485 490 495
Gly Lys Val Asp Pro Gln Asp Tyr Phe Arg Asn Glu Gln Ser Ile Pro
500 505 510
Pro Leu Val Glu Lys Tyr
515
<210>3
<211>1644
<212>DNA
<213>黑麦(Secale cereale)
<220>
<221>stop_codon
<222>(1561)..(1563)
<223>
<220>
<221>signal_sequence_DNA
<222>(1)..(66)
<223>
<220>
<221>signal_sequence_PROT
<222>(1)..(22)
<223>
<220>
<221>CDS
<222>(1)..(1563)
<223>
<400>3
aac tcg agg gcc ttt gct ctg gtg ccc ctc ctc atc tgc gtc ttg tcc 48
Asn Ser Arg Ala Phe Ala Leu Val Pro Leu Leu Ile Cys Val Leu Ser
1 5 10 15
tgc cac gcc gcc gtc tcc tac gcg gcg gcg ccg gtg ccg gcc aag gag 96
Cys His Ala Ala Val Ser Tyr Ala Ala Ala Pro Val Pro Ala Lys Glu
20 25 30
gac ttc ttc gga tgc ctg gtg aag gag ata ccg gcc cgc ctc ctc tac 144
Asp Phe Phe Gly Cys Leu Val Lys Glu Ile Pro Ala Arg Leu Leu Tyr
35 40 45
gcc aag agc tcg cct gcc ttc ccc acc gtc ctg gcg cag acc atc agg 192
Ala Lys Ser Ser Pro Ala Phe Pro Thr Val Leu Ala Gln Thr Ile Arg
50 55 60
aac tcg cgg tgg tcg tcg ccg cag agc gtg aag ccg ctc tac atc atc 240
Asn Ser Arg Trp Ser Ser Pro Gln Ser Val Lys Pro Leu Tyr Ile Ile
65 70 75 80
acc ccc acc aac gcc tcc cac atc cag tcc gcg gtg gtg tgc ggc cgc 288
Thr Pro Thr Asn Ala Ser His Ile Gln Ser Ala Val Val Cys Gly Arg
85 90 95
cgg cac ggc gtc cgc atc cgc gtg cgg agc ggc ggc cac gac tac gag 336
Arg His Gly Val Arg Ile Arg Val Arg Ser Gly Gly His Asp Tyr Glu
100 105 110
ggc ctg tcg tac cgg tcc gag cgc ccc gag gcg ttc gcc gtc gtc gac 384
Gly Leu Ser Tyr Arg Ser Glu Arg Pro Glu Ala Phe Ala Val Val Asp
115 120 125
ctc aac aag atg cgg gcc gtg gtg gtc gac ggc aag gct cgc acg gcg 432
Leu Asn Lys Met Arg Ala Val Val Val Asp Gly Lys Ala Arg Thr Ala
130 135 140
tgg gtg gac tcc ggt gcg cag ctc ggc gag ctc tac tac gcc atc gcc 480
Trp Val Asp Ser Gly Ala Gln Leu Gly Glu Leu Tyr Tyr Ala Ile Ala
145 150 155 160
aag aac agc ccc gtg ctc gcg ttc ccg gcc ggc gtt tgc ccg acc att 528
Lys Asn Ser Pro Val Leu Ala Phe Pro Ala Gly Val Cys Pro Thr Ile
165 170 175
ggt gta ggc ggc aac ttc gct ggc ggc ggc ttc ggc atg ctg ctg cgc 576
Gly Val Gly Gly Asn Phe Ala Gly Gly Gly Phe Gly Met Leu Leu Arg
180 185 190
aag tac ggc atc gcc gcc gag aac gtc atc gac gtg aag gtg gtc gac 624
Lys Tyr Gly Ile Ala Ala Glu Asn Val Ile Asp Val Lys Val Val Asp
195 200 205
gcc aac ggc aca ctg ctc gac aag agc tco atg agc gcg gat cac ttc 672
Ala Asn Gly Thr Leu Leu Asp Lys Ser Ser Met Ser Ala Asp His Phe
210 215 220
tgg gcc gtc agg ggc ggc ggc gga gag agc ttc ggc atc gtc gtg tcg 720
Trp Ala Val Arg Gly Gly Gly Gly Glu Ser Phe Gly Ile Val Val Ser
225 230 235 240
tgg cag gtg aag ctc ctc ccg gtg cct ccc acc gtg acc gtg ttc aag 768
Trp Gln Val Lys Leu Leu Pro Val Pro Pro Thr Val Thr Val Phe Lys
245 250 255
atc ccc aag acg gtg caa gaa ggc gcc gta gag ctc atc aac aag tgg 816
Ile Pro Lys Thr Val Gln Glu Gly Ala Val Glu Leu Ile Asn Lys Trp
260 265 270
cag cta gtc gcg ccg gcc ctc ccc gac gac ctg atg atc cgc atc atc 864
Gln Leu Val Ala Pro Ala Leu Pro Asp Asp Leu Met Ile Arg Ile Ile
275 280 285
gct ttc ggc ggc acc gcc aag ttc gag gcc atg tac ctg ggc acc tgc 912
Ala Phe Gly Gly Thr Ala Lys Phe Glu Ala Met Tyr Leu Gly Thr Cys
290 295 300
aaa gcc ctg aca ccg ctg atg agc agc aga ttc ccc gag ctc ggc atg 960
Lys Ala Leu Thr Pro Leu Met Ser Ser Arg Phe Pro Glu Leu Gly Met
305 310 315 320
aac gcc tcg cac tgc aac gag atg ccc tgg atc aag tcc gtc cca ttc 1008
Asn Ala Ser His Cys Asn Glu Met Pro Trp Ile Lys Ser Val Pro Phe
325 330 335
atc cac cttggc aag cag gcc acc ctc tcc gac ctc ctc aac cgg aac 1056
Ile His Leu Gly Lys Gln Ala Thr Leu Ser Asp Leu Leu Asn Arg Asn
340 345 350
aac acc ttc aaa ccc ttc gcc gag tac aag tcg gac tac gtc tac cag 1104
Asn Thr Phe Lys Pro Phe Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln
355 360 365
ccc gtc ccc aag ccc gtc tgg gcg cag atc ttc gtc tgg ctc gtc aaa 1152
Pro Val Pro Lys Pro Val Trp Ala Gln Ile Phe Val Trp Leu Val Lys
370 375 380
ccc ggc gcc ggg atc atg gtc atg gac ccc tac ggc gcc gcc atc agc 1200
Pro Gly Ala Gly Ile Met Val Met Asp Pro Tyr Gly Ala Ala Ile Ser
385 390 395 400
gcc acc ccc gaa gcc gcc acg ccg ttc cct cac cgc aag gac gtc ctc 1248
Ala Thr Pro Glu Ala Ala Thr Pro Phe Pro His Arg Lys Asp Val Leu
405 410 415
ttc aac atc cag tac gtc aac tac tgg ttc gac gag gca ggc ggc gcc 1296
Phe Asn Ile Gln Tyr Val Asn Tyr Trp Phe Asp Glu Ala Gly Gly Ala
420 425 430
gcg ccg ctg cag tgg agc aag gac atg tac agg ttc atg gag ccg tac 1344
Ala Pro Leu Gln Trp Ser Lys Asp Met Tyr Arg Phe Met Glu Pro Tyr
435 440 445
gtc agc aag aac ccc aga cag gcc tac gcc aac tac agg gac atc gac 1392
Val Ser Lys Asn Pro Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp
450 455 460
ctc ggc agg aac gag gtg gtc aac gac atc tcc acc tat gcc agc ggc 1440
Leu Gly Arg Asn Glu Val Val Asn Asp Ile Ser Thr Tyr Ala Ser Gly
465 470 475 480
aag gtc tgg ggc gag aag tac ttc aag ggc aac ttc caa agg ctc gcc 1488
Lys Val Trp Gly Glu Lys Tyr Phe Lys Gly Asn Phe Gln Arg Leu Ala
485 490 495
att acc aag ggc aag gtg gat cct cag gac tac ttc agg aac gag cag 1536
Ile Thr Lys Gly Lys Val Asp Pro Gln Asp Tyr Phe Arg Asn Glu Gln
500 505 510
agc atc ccg ccg ctg cta ggg aag tag tagtactctt gcttgcatgg 1583
Ser Ile Pro Pro Leu Leu Gly Lys
515 520
agatttgtag tgcgtctttc gcgtttcaaa tgcccaacta gtagaataag gatcgtgcgt 1643
a 1644
<210>4
<211>520
<212>PRT
<213>黑麦(Secale cereale)
<400>4
Asn Ser Arg Ala Phe Ala Leu Val Pro Leu Leu Ile Cys Val Leu Ser
1 5 10 15
Cys His Ala Ala Val Ser Tyr Ala Ala Ala Pro Val Pro Ala Lys Glu
20 25 30
Asp Phe Phe Gly Cys Leu Val Lys Glu Ile Pro Ala Arg Leu Leu Tyr
35 40 45
Ala Lys Ser Ser Pro Ala Phe Pro Thr Val Leu Ala Gln Thr Ile Arg
50 55 60
Asn Ser Arg Trp Ser Ser Pro Gln Ser Val Lys Pro Leu Tyr Ile Ile
65 70 75 80
Thr Pro Thr Asn Ala Ser His Ile Gln Ser ALa Val Val Cys Gly Arg
85 90 95
Arg His Gly Val Arg Ile Arg Val Arg Ser Gly Gly His Asp Tyr Glu
100 105 110
GlV Leu Ser Tyr Arg Ser Glu Arg Pro Glu Ala Phe Ala Val Val Asp
115 120 125
Leu Asn Lys Met Arg Ala Val Val Val Asp Gly Lys Ala Arg Thr Ala
130 135 140
Trp Val Asp Ser Gly Ala Gln Leu Gly Glu Leu Tyr Tyr Ala Ile Ala
145 150 155 160
Lys Asn Ser Pro Val Leu Ala Phe Pro Ala Gly Val Cys Pro Thr Ile
165 170 175
Gly Val Gly Gly Asn Phe Ala Gly Gly Gly Phe Gly Met Leu Leu Arg
180 185 190
Lys Tyr Gly Ile Ala Ala Glu Asn Val Ile Asp Val Lys Val Val Asp
195 200 205
Ala Asn Gly Thr Leu Leu Asp Lys Ser Ser Met Ser Ala Asp His Phe
210 215 220
Trp Ala Val Arg Gly Gly Gly Gly Glu Ser Phe Gly Ile Val Val Ser
225 230 235 240
Trp Gln Val Lys Leu Leu Pro Val Pro Pro Thr Val Thr Val Phe Lys
245 250 255
Ile Pro Lys Thr Val Gln Glu Gly Ala Val Glu Leu Ile Asn Lys Trp
260 265 270
Gln Leu Val Ala Pro Ala Leu Pro Asp Asp Leu Met Ile Arg Ile Ile
275 280 285
Ala Phe Gly Gly Thr Ala Lys Phe Glu Ala Met Tyr Leu Gly Thr Cys
290 295 300
Lys Ala Leu Thr Pro Leu Met Ser Ser Arg Phe Pro Glu Leu Gly Met
305 310 315 320
Asn Ala Ser His Cys Asn Glu Met Pro Trp Ile Lys Ser Val Pro Phe
325 330 335
Ile His Leu Gly Lys Gln Ala Thr Leu Ser Asp Leu Leu Asn Arg Asn
340 345 350
Asn Thr Phe Lys Pro Phe Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln
355 360 365
Pro Val Pro Lys Pro Val Trp Ala Gln Ile Phe Val Trp Leu Val Lys
370 375 380
Pro Gly Ala Gly Ile Met Val Met Asp Pro Tyr Gly Ala Ala Ile Ser
385 390 395 400
Ala Thr Pro Glu Ala Ala Thr Pro Phe Pro His Arg Lys Asp Val Leu
405 410 415
Phe Asn Ile Gln Tyr Val Asn Tyr Trp Phe Asp Glu Ala Gly Gly Ala
420 425 430
Ala Pro Leu Gln Trp Ser Lys Asp Met Tyr Arg Phe Met Glu Pro Tyr
435 440 445
Val Ser Lys Asn Pro Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp
450 455 460
Leu Gly Arg Asn Glu Val Val Asn Asp Ile Ser Thr Tyr Ala Ser Gly
465 470 475 480
Lys Val Trp Gly Glu Lys Tyr Phe Lys Gly Asn Phe Gln Arg Leu Ala
485 490 495
Ile Thr Lys Gly Lys Val Asp Pro Gln Asp Tyr Phe Arg Asn Glu Gln
500 505 510
Ser Ile Pro Pro Leu Leu Gly Lys
515 520
<210>5
<211>1608
<212>DNA
<213>大麦(Hordeum vulgare)
<220>
<221>stop_codon
<222>(1555)..(1557)
<223>
<220>
<221>signal_sequence_DNA
<222>(1)..(66)
<223>
<220>
<221>signal_sequence_PROT
<222>(1)..(22)
<223>
<220>
<221>CDS
<222>(1)..(1557)
<223>
<400>5
agc tcg agg gcc ttc gct ctg gtg ctc ctc ctc tgc gcc ttg tcc tgc 48
Ser Ser Arg Ala Phe Ala Leu Val Leu Leu Leu Cys Ala Leu Ser Cys
1 5 10 15
cac cac gct gcc gtc tcc tcc gcg cag gtg ccg gcc aag gac gac ttc 96
His His Ala Ala Val Ser Ser Ala Gln Val Pro Ala Lys Asp Asp Phe
20 25 30
ctg gga tgc ctc gtg aag gag ata ccg gcc cgc ctc ctc ttc gcc aag 144
Leu Gly Cys Leu Val Lys Glu Ile Pro Ala Arg Leu Leu Phe Ala Lys
35 40 45
agc tcg cct gcc ttc ccc gcc gtc ctg gag cag acc atc agg aac tcg 192
Ser Ser Pro Ala Phe Pro Ala Val Leu Glu Gln Thr Ile Arg Asn Ser
50 55 60
cgg tgg tcg tcg ccg cag aac gtg aag ccg ctc tac atc atc acc ccc 240
Arg Trp Ser Ser Pro Gln Asn Val Lys Pro Leu Tyr Ile Ile Thr Pro
65 70 75 80
acc aac acc tcc cac atc cag tct gct gtg gtg tgc ggc cgc cgg cac 288
Thr Asn Thr Ser His Ile Gln Ser Ala Val Val Cys Gly Arg Arg His
85 90 95
ggc gtc cgc ctc cgc gtg cgg agc ggc ggc cac gac tac gag ggc ctg 336
Gly Val Arg Leu Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu
100 105 110
tcg tac cgg tcc gag cgc ccc gag gcg ttc gcc gtc gta gac ctc aac 384
Ser Tyr Arg Ser Glu Arg Pro Glu Ala Phe Ala Val Val Asp Leu Asn
115 120 125
aag atg cgg acc gtg ttg gtc aac gaa aag gcc cgc acg gcg tgg gtg 432
Lys Met Arg Thr Val Leu Val Asn Glu Lys Ala Arg Thr Ala Trp Val
130 135 140
gac tcc ggc gcg cag ctc ggc gag ctc tac tac gcc atc gcc aag aac 480
Asp Ser Gly Ala Gln Leu Gly Glu Leu Tyr Tyr Ala Ile Ala Lys Asn
145 150 155 160
agc ccc gtg ctc gcg ttc cca gcc ggc gtt tgc ccg tcc att ggt gta 528
Ser Pro Val Leu Ala Phe Pro Ala Gly Val Cys Pro Ser Ile Gly Val
165 170 175
ggt ggc aac ttc gct ggc ggc ggc ttc ggc atg ctg ctg cgc aag tac 576
Gly Gly Asn Phe Ala Gly Gly Gly Phe Gly Met Leu Leu Arg Lys Tyr
180 185 190
ggc atc gcc gcc gag aac gtc atc gac gtc aag ctg gtc gac gcc aac 624
Gly Ile Ala Ala Glu Asn Val Ile Asp Val Lys Leu Val Asp Ala Asn
195 200 205
ggc aag ctg ctc gac aag agc tcc atg agc ccg gac cac ttc tgg gcc 672
Gly Lys Leu Leu Asp Lys Ser Ser Met Ser Pro Asp His Phe Trp Ala
210 215 220
gtc agg ggc ggc ggc gga gag agc ttc ggc atc gtc gtc tcg tgg cag 720
Val Arg Gly Gly Gly Gly Glu Ser Phe Gly Ile Val Val Ser Trp Gln
225 230 235 240
gtg aag ctt ctc ccg gtg cct ccc acc gtg act gtg ttt cag atc ccc 768
Val Lys Leu Leu Pro Val Pro Pro Thr Val Thr Val Phe Gln Ile Pro
245 250 255
aag aca gtg caa gaa ggc gcc gta gac ctc atc aac aag tgg cag ctg 816
Lys Thr Val Gln Glu Gly Ala Val Asp Leu Ile Asn Lys Trp Gln Leu
260 265 270
gtc gcg ccg gcc ctt ccc ggc gac atc atg atc cgc atc atc gcc atg 864
Val Ala Pro Ala Leu Pro Gly Asp Ile Met Ile Arg Ile Ile Ala Met
275 280 285
ggg gac aaa gcg acg ttc gag gcc atg tac ctg ggc acc tgc aaa acc 912
Gly Asp Lys Ala Thr Phe Glu Ala Met Tyr Leu Gly Thr Cys Lys Thr
290 295 300
ctg acg ccg ctg atg agc agc aaa ttc ccg gag ctt ggc atg aac cco 960
Leu Thr Pro Leu Met Ser Ser Lys Phe Pro Glu Leu Gly Met Asn Pro
305 310 315 320
tcg cac tgc aac gag atg ccc tgg atc aag tcc atc ccc ttc atc cac 1008
Ser His Cys Asn Glu Met Pro Trp Ile Lys Ser Ile Pro Phe Ile His
325 330 335
ctt ggc aag cag gcc acc ctg gcc gac ctc ctc aac cgg aac aac acc 1056
Leu Gly Lys Gln Ala Thr Leu Ala Asp Leu Leu Asn Arg Asn Asn Thr
340 345 350
ttc aaa ccc ttc gcc gaa tac aag tcg gac tac gtc tac cag ccc gtc 1104
Phe Lys Pro Phe Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln Pro Val
355 360 365
ccc aag ccc gtg tgg gag cag ctc ttc ggc tgg ctc acg aaa ccc ggc 1152
Pro Lys Pro Val Trp Glu Gln Leu Phe Gly Trp Leu Thr Lys Pro Gly
370 375 380
gcg ggg atc atg gtc atg gac cca tac ggc gcc acc atc agc gcc acc 1200
Ala Gly Ile Met Val Met Asp Pro Tyr Gly Ala Thr Ile Ser Ala Thr
385 390 395 400
ccc gaa gcg gcg acg ccg ttc cct cac cgc aag ggc gtc ctc ttc aac 1248
Pro Glu Ala Ala Thr Pro Phe Pro His Arg Lys Gly Val Leu Phe Asn
405 410 415
atc cag tac gtc aac tac tgg ttc gcc gag gca gcc ggc gcc gcg ccg 1296
Ile Gln Tyr Val Asn Tyr Trp Phe Ala Glu Ala Ala Gly Ala Ala Pro
420 425 430
ctg cag tgg agc aag gac att tac aaa ttc atg gag ccg ttc gtg agc 1344
Leu Gln Trp Ser Lys Asp Ile Tyr Lys Phe Met Glu Pro Phe Val Ser
435 440 445
aag aac ccc agg cag gcg tac gcc aac tac agg gsc atc gac ctc ggc 1392
Lys Asn Pro Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp Leu Gly
450 455 460
agg aac gag gtg gtg aac gac atc tca acc tac agc agc ggc aag gtg 1440
Arg Asn Glu Val Val Asn Asp Ile Ser Thr Tyr Ser Ser Gly Lys Val
465 470 475 480
tgg ggc gag aag tac ttc aag ggc aac ttc caa agg ctc gcc atc acc 1488
Trp Gly Glu Lys Tyr Phe Lys Gly Asn Phe Gln Arg Leu Ala Ile Thr
485 490 495
aag ggc aag gtg gat ccc cag gac tac ttc agg aac gag cag agc atc 1536
Lys Gly Lys Val Asp Pro Gln Asp Tyr Phe Arg Asn Glu Gln Ser Ile
500 505 510
ccg ccg ctg ctg ggc aag tag tgaccgagag tcttgcatgg agatttgtag 1587
Pro Pro Leu Leu Gly Lys
515
tgcgtgcttg gcgtttctga t 1608
<210>6
<211>518
<212>PRT
<213>大麦(Hordeum vulgare)
<400>6
Ser Ser Arg Ala Phe Ala Leu Val Leu Leu Leu Cys Ala Leu Ser Cys
1 5 10 15
His His Ala Ala Val Ser Ser Ala Gln Val Pro Ala Lys Asp Asp Phe
20 25 30
Leu Gly Cys Leu Val Lys Glu Ile Pro Ala Arg Leu Leu Phe Ala Lys
35 40 45
Ser Ser Pro Ala Phe Pro Ala Val Leu Glu Gln Thr Ile Arg Asn Ser
50 55 60
Arg Trp Ser Ser Pro Gln Asn Val Lys Pro Leu Tyr Ile Ile Thr Pro
65 70 75 80
Thr Asn Thr Ser His Ile Gln Ser Ala Val Val Cys Gly Arg Arg His
85 90 95
Gly Val Arg Leu Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu
100 105 110
Ser Tyr Arg Ser Glu Arg Pro Glu Ala Phe Ala Val Val Asp Leu Asn
115 120 125
Lys Met Arg Thr Val Leu Val Asn Glu Lys Ala Arg Thr Ala Trp Val
130 135 140
Asp Ser Gly Ala Gln Leu Gly Glu Leu Tyr Tyr Ala Ile Ala Lys Asn
145 150 155 160
Ser Pro Val Leu Ala Phe Pro Ala Gly Val Cys Pro Ser Ile Gly Val
165 170 175
Gly Gly Asn Phe Ala Gly Gly Gly Phe Gly Met Leu Leu Arg Lys Tyr
180 185 190
Gly Ile Ala Ala Glu Asn Val Ile Asp Val Lys Leu Val Asp Ala Asn
195 200 205
Gly Lys Leu Leu Asp Lys Ser Ser Met Ser Pro Asp His Phe Trp Ala
210 215 220
Val Arg Gly Gly Gly Gly Glu Ser Phe Gly Ile Val Val Ser Trp Gln
225 230 235 240
Val Lys Leu Leu Pro Val Pro Pro Thr Val Thr Val Phe Gln Ile Pro
245 250 255
Lys Thr Val Gln Glu Gly Ala Val Asp Leu Ile Asn Lys Trp Gln Leu
260 265 270
Val Ala Pro Ala Leu Pro Gly Asp Ile Met Ile Arg Ile Ile Ala Met
275 280 285
Gly Asp Lys Ala Thr Phe Glu Ala Met Tyr Leu Gly Thr Cys Lys Thr
290 295 300
Leu Thr Pro Leu Met Ser Ser Lys Phe Pro Glu Leu Gly Met Asn Pro
305 310 315 320
Ser His Cys Asn Glu Met Pro Trp Ile Lys Ser Ile Pro Phe Ile His
325 330 335
Leu Gly Lys Gln Ala Thr Leu Ala Asp Leu Leu Asn Arg Asn Asn Thr
340 345 350
Phe Lys Pro Phe Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln Pro Val
355 360 365
Pro Lys Pro Val Trp Glu Gln Leu Phe Gly Trp Leu Thr Lys Pro Gly
370 375 380
Ala Gly Ile Met Val Met Asp Pro Tyr Gly Ala Thr Ile Ser Ala Thr
385 390 395 400
Pro Glu Ala Ala Thr Pro Phe Pro His Arg Lys Gly Val Leu Phe Asn
405 410 415
Ile Gln Tyr Val Asn Tyr Trp Phe Ala Glu Ala Ala Gly Ala Ala Pro
420 425 430
Leu Gln Trp Ser Lys Asp Ile Tyr Lys Phe Met Glu Pro Phe Val Ser
435 440 445
Lys Asn Pro Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp Leu Gly
450 455 460
Arg Asn Glu Val Val Asn Asp Ile Ser Thr Tyr Ser Ser Gly Lys Val
465 470 475 480
Trp Gly Glu Lys Tyr Phe Lys Gly Asn Phe Gln Arg Leu Ala Ile Thr
485 490 495
Lys Gly Lys Val Asp Pro Gln Asp Tyr Phe Arg Asn Glu Gln Ser Ile
500 505 510
Pro Pro Leu Leu Gly Lys
515
<210>7
<211>1603
<212>DNA
<213>小麦(Triticum aestivum)
<220>
<221>stop_codon
<222>(1555)..(1557)
<223>
<220>
<221>signal_sequence_DNA
<222>(1)..(63)
<223>
<220>
<221>signal_sequence_PROT
<222>(1)..(21)
<223>
<220>
<221>CDS
<222>(1)..(1557)
<223>
<400>7
aac tat agg gcc ttc acg ctg gtg ctc ctc ttc tgc gcc ttg tcc tgt 48
Asn Tyr Arg Ala Phe Thr Leu Val Leu Leu Phe Cys Ala Leu Ser Cys
1 5 10 15
caa gcc gcc gcc acc tac gcg ccg gtg cct gcc aag gag gac ttc ctc 96
Gln Ala Ala Ala Thr Tyr Ala Pro Val Pro Ala Lys Glu Asp Phe Leu
20 25 30
gga tgc ctc atg aag gag ata ccg gca cgc ctc ctc tac gcc aag agc 144
Gly Cys Leu Met Lys Glu Ile Pro Ala Arg Leu Leu Tyr Ala Lys Ser
35 40 45
tcg cct gacttc ccc acc gtc ctg gcg cag acc atc agg aac tcg cgg 192
Ser Pro Asp Phe Pro Thr Val Leu Ala Gln Thr Ile Arg Asn Ser Arg
50 55 60
tgg ttg tcg ccg cag aac gtg aag ccg ctc tac atc atc acc ccc acc 240
Trp Leu Ser Pro Gln Asn Val Lys Pro Leu Tyr Ile Ile Thr Pro Thr
65 70 75 80
aac gcc tcg cac atc cag tcc gcg gtg gtg tgc gga cgc cgg cac agc 288
Asn Ala Ser His Ile Gln Ser Ala Val Val Cys Gly Arg Arg His Ser
85 90 95
gtc cgc ctc cgc gtc cgg agc ggc ggc cac gac tac gag ggc ctg tcg 336
Val Arg Leu Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser
100 105 110
tac cgg tcc gag aaa ccc gag acg ttc gcc gtc gtc gac ctc aac aag 384
Tyr Arg Ser Glu Lys Pro Glu Thr Phe Ala Val Val Asp Leu Asn Lys
115 120 125
atg cgg gca gtg ttg atc gac ggc tac gcc cgc acg gcg tgg gtc gaa 432
Met Arg Ala Val Leu Ile Asp Gly Tyr Ala Arg Thr Ala Trp Val Glu
130 135 140
tcc ggc gcg cag ctc ggc gag ctc tac tac gcc atc gcg aaa aac agc 480
Ser Gly Ala Gln Leu Gly Glu Leu Tyr Tyr Ala Ile Ala Lys Asn Ser
145 150 155 160
ccc gtg ctc gcg ttc ccg gcc ggc gtc tgc ccg acc atc ggc gtc ggc 528
Pro Val Leu Ala Phe Pro Ala Gly Val Cys Pro Thr Ile Gly Val Gly
165 170 175
ggc aac ttc gca ggc ggc ggc ttt ggc atg ctg ctg cgg aag tac ggc 576
Gly Asn Phe Ala Gly Gly Gly Phe Gly Met Leu Leu Arg Lys Tyr Gly
180 185 190
atc gcc gcc gag aac gtc atc gac gtc aag gtg gtc gac ccc aac ggc 624
Ile Ala Ala Glu Asn Val Ile Asp Val Lys Val Val Asp Pro Asn Gly
195 200 205
aag ctt ctc gac aag agc tcc atg agc ccg gac cac ttc tgg gcc gtc 672
Lys Leu Leu Asp Lys Ser Ser Met Ser Pro Asp His Phe Trp Ala Val
210 215 220
agg ggc ggc ggc gga gag agc ttt ggc atc gtc gtg tcg tgg caa gtg 720
Arg Gly Gly Gly Gly Glu Ser Phe GlyIle Val Val Ser Trp Gln Val
225 230 235 240
aag ctc ctg ccg gtg cct ccc acc gtg acc gtg ttc aag atc ccc aag 768
Lys Leu Leu Pro Val Pro Pro Thr Val Thr Val Phe Lys Ile Pro Lys
245 250 255
aca gtg caa gaa ggc gcc gta gac ctc gtc aac aag tgg caa ctg gtc 816
Thr Val Gln Glu Gly Ala Val Asp Leu Val Asn Lys Trp Gln Leu Val
260 265 270
ggg ccg gcc ctt ccc ggc gac ctc atg atc cgc gtc atc gct gcg ggg 864
Gly Pro Ala Leu Pro Gly Asp Leu Met Ile Arg Val Ile Ala Ala Gly
275 280 285
aac acc gcg aca ttc gag ggc atg tac ctg ggc acc tgc caa acc ctg 912
Asn Thr Ala Thr Phe Glu Gly Met Tyr Leu Gly Thr Cys Gln Thr Leu
290 295 300
acg ccg ttg atg agc agc caa ttc ccc gag ctt ggc atg aac ccc tat 960
Thr Pro Leu Met Ser Ser Gln Phe Pro Glu Leu Gly Met Asn Pro Tyr
305 310 315 320
cac tgc aac gag atg ccc tgg atc aag tcc atc ccc ttc atc cac ctc 1008
His Cys Asn Glu Met Pro Trp Ile Lys Ser Ile Pro Phe Ile His Leu
325 330 335
ggc aaa gag gcc agc ctg gtc gac ctc ctc aac cgg aac aac acc ttc 1056
Gly Lys Glu Ala Ser Leu Val Asp Leu Leu Asn Arg Asn Asn Thr Phe
340 345 350
aag ccc ttc gcc gaa tac aag tcg gac tac gtg tac cag ccc ttc ccc 1104
Lys Pro Phe Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln Pro Phe Pro
355 360 365
aag ccc gtg tgg gag cag atc ttc ggc tgg ctc acg aag ccc ggt ggg 1152
Lys Pro Val Trp Glu Gln Ile Phe Gly Trp Leu Thr Lys Pro Gly Gly
370 375 380
ggg atg atg atc atg gac cca tac ggc gcc acc atc agc gcc acc ccc 1200
Gly Met Met Ile Met Asp Pro Tyr Gly Ala Thr Ile Ser Ala Thr Pro
385 390 395 400
gaa gcg gcg acg ccg ttc cct cac cgc cag ggc gtt ctc ttc aao atc 1248
Glu Ala Ala Thr Pro Phe Pro His Arg Gln Gly Val Leu Phe Asn Ile
405 410 415
cag tac gtc aac tac tgg ttc gcc gag gca gcc gcc gcc gcg ccg ctg 1296
Gln Tyr Val Asn Tyr Trp Phe Ala Glu Ala Ala Ala Ala Ala Pro Leu
420 425 430
cag tgg agc aag gac atg tac aat ttc atg gag ccg tac gtg agc aag 1344
Gln Trp Ser Lys Asp Met Tyr Asn Phe Met Glu Pro Tyr Val Ser Lys
435 440 445
aac ccc agg cag gcg tac gcc aac tac agg gac att gac ctc ggc agg 1392
Asn Pro Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp Leu Gly Arg
450 455 460
aac gag gtg gtg aac gac atc tca acc tat agc agc ggc aag gtt tgg 1440
Asn Glu Val Val Asn Asp Ile Ser Thr Tyr Ser Ser Gly Lys Val Trp
465 470 475 480
ggc gag aag tac ttc aag ggc aac ttc caa agg ctc gct att acc aag 1488
Gly Glu Lys Tyr Phe Lys Gly Asn Phe Gln Arg Leu Ala Ile Thr Lys
485 490 495
ggc aag gtg gat cct cag gac tac ttc agg aac gag cag agc atc ccg 1536
Gly Lys Val Asp Pro Gln Asp Tyr Phe Arg Asn Glu Gln Ser Ile Pro
500 505 510
ccg ctg ctc gag aag tac tga tcgaggacct tgcatggaga tttagtgcgt 1587
Pro Leu Leu Glu Lys Tyr
515
ggttgccgtt tcacat 1603
<210>8
<211>518
<212>PRT
<213>小麦(Triticum aestivum)
<400>8
Asn Tyr Arg Ala Phe Thr Leu Val Leu Leu Phe Cys Ala Leu Ser Cys
1 5 10 15
Gln Ala Ala Ala Thr Tyr Ala Pro Val Pro Ala Lys Glu Asp Phe Leu
20 25 30
Gly Cys Leu Met Lys Glu Ile Pro Ala Arg Leu Leu Tyr Ala Lys Ser
35 40 45
Ser Pro Asp Phe Pro Thr Val Leu Ala Gln Thr Ile Arg Asn Ser Arg
50 55 60
Trp Leu Ser Pro Gln Asn Val Lys Pro Leu Tyr Ile Ile Thr Pro Thr
65 70 75 80
Asn Ala Ser His Ile Gln Ser Ala Val Val Cys Gly Arg Arg His Ser
85 90 95
Val Arg Leu Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser
100 105 110
Tyr Arg Ser Glu Lys Pro Glu Thr Phe Ala Val Val Asp Leu Asn Lys
115 120 125
Met Arg Ala Val Leu Ile Asp Gly Tyr Ala Arg Thr Ala Trp Val Glu
130 135 140
Ser Gly Ala Gln Leu Gly Glu Leu Tyr Tyr Ala Ile Ala Lys Asn Ser
145 150 155 160
Pro Val Leu Ala Phe Pro Ala Gly Val Cys Pro Thr Ile Gly Val Gly
165 170 175
Gly Asn Phe Ala Gly Gly Gly Phe Gly Met Leu Leu Arg Lys Tyr Gly
180 185 190
Ile Ala Ala Glu Asn Val Ile Asp Val Lys Val Val Asp Pro Asn Gly
195 200 205
Lys Leu Leu Asp Lys Ser Ser Met Ser Pro Asp His Phe Trp Ala Val
210 215 220
Arg Gly Gly Gly Gly Glu Ser Phe Gly Ile Val Val Ser Trp Gln Val
225 230 235 240
Lys Leu Leu Pro Val Pro Pro Thr Val Thr Val Phe Lys Ile Pro Lys
245 250 255
Thr Val Gln Glu Gly Ala Val Asp Leu Val Asn Lys Trp Gln Leu Val
260 265 270
Gly Pro Ala Leu Pro Gly Asp Leu Met Ile Arg Val Ile Ala Ala Gly
275 280 285
Asn Thr Ala Thr Phe Glu Gly Met Tyr Leu Gly Thr Cys Gln Thr Leu
290 295 300
Thr Pro Leu Met Ser Ser Gln Phe Pro Glu Leu Gly Met Asn Pro Tyr
305 310 315 320
His Cys Asn Glu Met Pro Trp Ile Lys Ser Ile Pro Phe Ile His Leu
325 330 335
Gly Lys Glu Ala Ser Leu Val Asp Leu Leu Asn Arg Asn Asn Thr Phe
340 345 350
Lys Pro Phe Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln Pro Phe Pro
355 360 365
Lys Pro Val Trp Glu Gln Ile Phe Gly Trp Leu Thr Lys Pro Gly Gly
370 375 380
Gly Met Met Ile Met Asp Pro Tyr Gly Ala Thr Ile Ser Ala Thr Pro
385 390 395 400
Glu Ala Ala Thr Pro Phe Pro His Arg Gln Gly Val Leu Phe Asn Ile
405 410 415
Gln Tyr Val Asn Tyr Trp Phe Ala Glu Ala Ala Ala Ala Ala Pro Leu
420 425 430
Gln Trp Ser Lys Asp Met Tyr Asn Phe Met Glu Pro Tyr Val Ser Lys
435 440 445
Asn Pro Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp Leu Gly Arg
450 455 460
Asn Glu Val Val Asn Asp Ile Ser Thr Tyr Ser Ser Gly Lys Val Trp
465 470 475 480
Gly Glu Lys Tyr Phe Lys Gly Asn Phe Gln Arg Leu Ala Ile Thr Lys
485 490 495
Gly Lys Val Asp Pro Gln Asp Tyr Phe Arg Asn Glu Gln Ser Ile Pro
500 505 510
Pro Leu Leu Glu Lys Tyr
515
<210>9
<211>1603
<212>DNA
<213>小麦(Triticum aestivum)
<220>
<221>stop_Codon
<222>(1555)..(1557)
<223>
<220>
<221>CDS
<222>(1)..(1557)
<223>
<220>
<221>signal_sequence_DNA
<222>(1)..(63)
<223>
<220>
<221>signal_sequence_PROT
<222>(1)..(21)
<223>
<400>9
aac tgt agg gcc ttc gcg cag gtg ctc ctc ttc ttc gcc ttg tcc tgc 48
Asn Cys Arg Ala Phe Ala Gln Val Leu Leu Phe Phe Ala Leu Ser Cys
1 5 10 15
caa gcc gcc gcc acc tac gcg ccg gtg cct gcc aag gag gac ttc ctc 96
Gln Ala Ala Ala Thr Tyr Ala Pro Val Pro Ala Lys Glu Asp Phe Leu
20 25 30
gga tgc ctc atg aag gag ata ccg gcc cgc ctc ctc tac gcc aag agc 144
Gly Cys Leu Met Lys Glu Ile Pro Ala Arg Leu Leu Tyr Ala Lys Ser
35 40 45
tcg cctgac tac ccc acc gtg ctg gcg cag acc atc agg aac tcg cgg 192
Ser Pro Asp Tyr Pro Thr Val Leu Ala Gln Thr Ile Arg Asn Ser Arg
50 55 60
tgg tcg acg cag cag aac gtg aag ccg ctg tac atc atc acc ccc acc 240
Trp Ser Thr Gln Gln Asn Val Lys Pro Leu Tyr Ile Ile Thr Pro Thr
65 70 75 80
aac gcc tcc cac atc caa tcc gcg gtg gtg tgc ggc cgc cgg cac ggc 288
Asn Ala Ser His Ile Gln Ser Ala Val Val Cys Gly Arg Arg His Gly
85 90 95
gtc cgc ctc cgc gtg cgg agc ggc ggc cac gac tac gag ggc ctg tcg 336
Val Arg Leu Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser
100 105 110
tac cgg tcc gag aaa ccc gag acg ttc gcc gtc gtc gac ctc aac aag 384
Tyr Arg Ser Glu Lys Pro Glu Thr Phe Ala Val Val Asp Leu Asn Lys
115 120 125
atg cgg gca gtg gtt gtc gac ggc tac gcc cgc acg gcg tgg gtc gaa 432
Met Arg Ala Val Val Val Asp Gly Tyr Ala Arg Thr Ala Trp Val Glu
130 135 140
tcc ggc gcg cag ctc ggc gag ctc tac tac gcc atc gcg aag aac agc 480
Ser Gly Ala Gln Leu Gly Glu Leu Tyr Tyr Ala Ile Ala Lys Asn Ser
145 150 155 160
ccc gtg ctc gcg ttc ccg gcc ggc gtc tgc ccg tcc atc ggc gtc ggc 528
Pro Val Leu Ala Phe Pro Ala Gly Val Cys Pro Ser Ile Gly Val Gly
165 170 175
ggc aac ttc gca ggc ggc ggc ttc ggc atg ctg ctg cgc aag tac ggc 576
Gly Asn Phe Ala Gly Gly Gly Phe Gly Met Leu Leu Arg Lys Tyr Gly
180 185 190
atc gcc gcc gag aac gtc atc gac gtc aag gtg gtc gac ccc gac ggc 624
Ile Ala Ala Glu Asn Val Ile Asp Val Lys Val Val Asp Pro Asp Gly
195 200 205
aag ctg ctc gac aag agc tcc atg agc gcg gac cac ttc tgg gcc gtc 672
Lys Leu Leu Asp Lys Ser Ser Met Ser Ala Asp His Phe Trp Ala Val
210 215 220
agg ggc ggc ggc gga gag agc ttc ggc atc gtc gtc tcg tgg cag gtg 720
Arg Gly Gly Gly Gly Glu Ser Phe Gly Ile Val Val Ser Trp Gln Val
225 230 235 240
aag ctc atg cca gtg cct ccc acc gtc acc gtg ttt aag atc ccc aag 768
Lys Leu Met Pro Val Pro Pro Thr Val Thr Val Phe Lys Ile Pro Lys
245 250 255
acg gtg caa gaa ggc gcc gta gac ctc gtc aac aag tgg cag ctg gtc 816
Thr Val Gln Glu Gly Ala Val Asp Leu Val Asn Lys Trp Gln Leu Val
260 265 270
ggg ccg gca ctt ccc ggc gac ctc atg atc cgc gtc atc gct gcc ggg 864
Gly Pro Ala Leu Pro Gly Asp Leu Met Ile Arg Val Ile Ala Ala Gly
275 280 285
aac acg 9cg acg ttc gag gcc ttg tac ctg ggc acc tgc aaa acc ctg 912
Asn Thr Ala Thr Phe Glu Ala Leu Tyr Leu Gly Thr Cys Lys Thr Leu
290 295 300
acg ccg ctg atg agc agc caa ttc ccc gag ctt ggc atg aac ccc tat 960
Thr Pro Leu Met Ser Ser Gln Phe Pro Glu Leu Gly Met Asn Pro Tyr
305 310 315 320
cac tgc aac gag atg ccc tgg atc aag tcc gtc ccc ttc atc cac ctc 1008
His Cys Asn Glu Met Pro Trp Ile Lys Ser Val Pro Phe Ile His Leu
325 330 335
ggc aaa cag gct ggc ctg gac gac ctc ctc aac cgg aac aac acc ttc 1056
Gly Lys Gln Ala Gly Leu Asp Asp Leu Leu Asn Arg Asn Asn Thr Phe
340 345 350
aag ccc ttc gcc gaa tac aag tcg gac tac gtg tac cag ccc ttc ccc 1104
Lys Pro Phe Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln Pro Phe Pro
355 360 365
aag ccc gtg tgg gag cag atc ttc ggc tgg ctc gcg aag ccc ggc gcg 1152
Lys Pro Val Trp Glu Gln Ile Phe Gly Trp Leu Ala Lys Pro Gly Ala
370 375 380
ggg atc atg atc atg gac ccc tac ggc gcc acc atc agc gcc acc ccc 1200
Gly Ile Met Ile Met Asp Pro Tyr Gly Ala Thr Ile Ser Ala Thr Pro
385 390 395 400
gaa gcg gcg acg ccg ttc cct cac cgc cag ggc gtc ctc ttc aac atc 1248
Glu Ala Ala Thr Pro Phe Pro His Arg Gln Gly Val Leu Phe Asn Ile
405 410 415
cag tat gtc aac tac tgg ttc gcc gag cca gcc ggc gcc gcg ccg ctg 1296
Gln Tyr Val Asn Tyr Trp Phe Ala Glu Pro Ala Gly Ala Ala Pro Leu
420 425 430
cag tgg agc aag gac att tac aat ttc atg gag ccg tac gtg agc aag 1344
Gln Trp Ser Lys Asp Ile Tyr Asn Phe Met Glu Pro Tyr Val Ser Lys
435 440 445
aac ccc agg cag gcg tac gcc aac tac agg gac atc gac ctc ggc agg 1392
Asn Pro Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp Leu Gly Arg
450 455 460
aat gag gtg gtg aac gac atc tca acc tac agc agc ggc aag gtg tgg 1440
Asn Glu ValVal Asn Asp Ile Ser Thr Tyr Ser Ser Gly Lys Val Trp
465 470 475 480
ggc gag aag tac ttc aag agc aac ttc caa agg ctc gcc att acc aag 1488
Gly Glu Lys Tyr Phe Lys Ser Asn Phe Gln Arg Leu Ala Ile Thr Lys
485 490 495
ggc aag gta gat cct cag gac tac ttc agg aat gag caa agc atc ccg 1536
Gly Lys Val Asp Pro Gln Asp Tyr Phe Arg Asn Glu Gln Ser Ile Pro
500 505 510
ccg ctg atc gag aag tac tga tcgaggacct tgcatggaga tttagtgcgt 1587
Pro Leu Ile Glu Lys Tyr
515
ggttggcgtt tcacat 1603
<210>10
<211>518
<212>PRT
<213>小麦(Triticum aestivum)
<400>10
Asn Cys Arg Ala Phe Ala Gln Val Leu Leu Phe Phe Ala Leu Ser Cys
1 5 10 15
Gln Ala Ala Ala Thr Tyr Ala Pro Val Pro Ala Lys Glu Asp Phe Leu
20 25 30
Gly Cys Leu Met Lys Glu Ile Pro Ala Arg Leu Leu Tyr Ala Lys Ser
35 40 45
Ser Pro Asp Tyr Pro Thr Val Leu Ala Gln Thr Ile Arg Asn Ser Arg
50 55 60
Trp Ser Thr Gln Gln Asn Val Lys Pro Leu Tyr Ile Ile Thr Pro Thr
65 70 75 80
Asn Ala Ser His Ile Gln Ser Ala Val Val Cys Gly Arg Arg His Gly
85 90 95
Val Arg Leu Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser
100 105 110
Tyr Arg Ser Glu Lys Pro Glu Thr Phe Ala Val Val Asp Leu Asn Lys
115 120 125
Met Arg Ala Val Val Val Asp Gly Tyr Ala Arg Thr Ala Trp Val Glu
130 135 140
Ser Gly Ala Gln Leu Gly Glu Leu Tyr Tyr Ala Ile Ala Lys Asn Ser
145 150 155 160
Pro Val Leu Ala Phe Pro Ala Gly Val Cys Pro Ser Ile Gly Val Gly
165 170 175
Gly Asn Phe Ala Gly Gly Gly Phe Gly Met Leu Leu Arg Lys Tyr Gly
180 185 190
Ile Ala Ala Glu Asn Val Ile Asp Val Lys Val Val Asp Pro Asp Gly
195 200 205
Lys Leu Leu Asp Lys Ser Ser Met Ser Ala Asp His Phe Trp Ala Val
210 215 220
Arg Gly Gly Gly Gly Glu Ser Phe Gly Ile Val Val Ser Trp Gln Val
225 230 235 240
Lys Leu Met Pro Val Pro Pro Thr Val Thr Val Phe Lys Ile Pro Lys
245 250 255
Thr Val Gln Glu Gly Ala Val Asp Leu Val Asn Lys Trp Gln Leu Val
260 265 270
Gly Pro Ala Leu Pro Gly Asp Leu Met Ile Arg Val Ile Ala Ala Gly
275 280 285
Asn Thr Ala Thr Phe Glu Ala Leu Tyr Leu Gly Thr Cys Lys Thr Leu
290 295 300
Thr Pro Leu Met Ser Ser Gln Phe Pro Glu Leu Gly Met Asn Pro Tyr
305 310 315 320
His Cys Asn Glu Met Pro Trp Ile Lys Ser Val Pro Phe Ile His Leu
325 330 335
Gly Lys Gln Ala Gly Leu Asp Asp Leu Leu Asn Arg Asn Asn Thr Phe
340 345 350
Lys Pro Phe Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln Pro Phe Pro
355 360 365
Lys Pro Val Trp Glu Gln Ile Phe Gly Trp Leu Ala Lys Pro Gly Ala
370 375 380
Gly Ile Met Ile Met Asp Pro Tyr Gly Ala Thr Ile Ser Ala Thr Pro
385 390 395 400
Glu Ala Ala Thr Pro Phe Pro His Arg Gln Gly Val Leu Phe Asn Ile
405 410 415
Gln Tyr Val Asn Tyr Trp Phe Ala Glu Pro Ala Gly Ala Ala Pro Leu
420 425 430
Gln Trp Ser Lys Asp Ile Tyr Asn Phe Met Glu Pro Tyr Val Ser Lys
435 440 445
Asn Pro Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp Leu Gly Arg
450 455 460
Asn Glu Val Val Asn Asp Ile Ser Thr Tyr Ser Ser Gly Lys Val Trp
465 470 475 480
Gly Glu Lys Tyr Phe Lys Ser Asn Phe Gln Arg Leu Ala Ile Thr Lys
485 490 495
Gly Lys Val Asp Pro Gln Asp Tyr Phe Arg Asn Glu Gln Ser Ile Pro
500 505 510
Pro Leu Ile Glu Lys Tyr
515
<210>11
<211>1503
<212>DNA
<213>梯牧草(Phleum pratense)
<220>
<221>CDS
<222>(1)..(1503)
<223>
<400>11
tac ttc ccg ccg ccg gct gct aaa gaa gac ttc ctg ggt tgc ctg gtt 48
Tyr Phe Pro Pro Pro Ala Ala Lys Glu Asp Phe Leu Gly Cys Leu Val
1 5 10 15
aaa gaa atc ccg ccg cgt ctg ttg tac gcg aaa tcg tcg ccg gcg tat 96
Lys Glu Ile Pro Pro Arg Leu Leu Tyr Ala Lys Ser Ser Pro Ala Tyr
20 25 30
ccc tca gtc ctg ggg cag acc atc cgg aac tcg agg tgg tcg tcg ccg 144
Pro Ser Val Leu Gly Gln Thr Ile Arg Asn Ser Arg Trp Ser Ser Pro
35 40 45
gac aac gtg aag ccg ctc tac atc atc acc ccc acc aac gtc tcc cac 192
Asp Asn Val Lys Pro Leu Tyr Ile Ile Thr Pro Thr Asn Val Ser His
50 55 60
atc cag tcc gcc gtg gtg tgc ggc cgc cgc cac agc gtc cgc atc cgc 240
Ile Gln Ser Ala Val Val Cys Gly Arg Arg His Ser Val Arg Ile Arg
65 70 75 80
gtg cgc agc ggc ggg cac gac tac gag ggc ctc tcg tac cgg tct ttg 288
Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr Arg Ser Leu
85 90 95
cag ccc gag acg ttc gcc gtc gtc gac ctc aac aag atg cgg gcg gtg 336
Gln Pro Glu Thr Phe Ala Val Val Asp Leu Asn Lys Met Arg Ala Val
100 105 110
tgg gtg gac ggc aag gcc cgc acg gcg tgg gtg gac tcc ggc gcg cag 384
Trp Val Asp Gly Lys Ala Arg Thr Ala Trp Val Asp Ser Gly Ala Gln
115 120 125
ctc ggc gag ctc tac tac gcc atc tat aag gcg agc ccc acg ctg gcg 432
Leu Gly Glu Leu Tyr Tyr Ala Ile Tyr Lys Ala Ser Pro Thr Leu Ala
130 135 140
ttc ccg gcc ggc gtg tgc ccg acg atc gga gtg ggc ggc aac ttc gcg 480
Phe Pro Ala Gly Val Cys Pro Thr Ile Gly Val Gly Gly Asn Phe Ala
145 150 155 l60
ggc ggc ggc ttc ggc atg ctg ctg cgc aag tac ggc atc gcc gcg gag 528
Gly Gly Gly Phe Gly Met Leu Leu Arg Lys Tyr Gly Ile Ala Ala Glu
165 170 175
aac gtc atc gac gtg aag ctc gtc gac gcc aac ggc aag ctg cac gac 576
Asn Val Ile Asp Val Lys Leu Val Asp Ala Asn Gly Lys Leu His Asp
180 185 190
aag aag tcc atg ggc gac gac cat ttc tgg gcc gtc agg ggc ggc ggg 624
Lys Lys Ser Met Gly Asp Asp His Phe Trp Ala Val Arg Gly Gly Gly
195 200 205
ggc gag agc ttc ggc ate gtg gtc gcg tgg cag gtg aag ctc ctg ccg 672
Gly Glu Ser Phe Gly Ile Val Val Ala Trp Gln Val Lys Leu Leu Pro
210 215 220
gtg ccg ccc acc gtg aca ata ttc aag atc tcc aag aca gtg agc gag 720
Val Pro Pro Thr Val Thr Ile Phe Lys Ile Ser Lys Thr Val Ser Glu
225 230 235 240
ggc gcc gtg gac atc atc aac aag tgg caa gtg gtc gcg ccg cag ctt 768
Gly Ala Val Asp Ile Ile Asn Lys Trp Gln Val Val Ala Pro Gln Leu
245 250 255
ccc gcc gac ctc atg atc cgc atc atc gcg cag ggg ccc aag gcc acg 816
Pro Ala Asp Leu Met Ile Arg Ile Ile Ala Gln Gly Pro Lys Ala Thr
260 265 270
ttc gag gcc atg tac ctc ggc acc tgc aaa acc ctg acg ccg ttg atg 864
Phe Glu Ala Met Tyr Leu Gly Thr Cys Lys Thr Leu Thr Pro Leu Met
275 280 285
agc agc aag ttc ccg gag ctc ggc atg aac ccc tcc cac tgc aac gag 912
Ser Ser Lys Phe Pro Glu Leu Gly Met Asn Pro Ser His Cys Asn Glu
290 295 300
atg tca tgg atc cag tcc atc ccc ttc gtc cac ctc ggc cac agg gac 960
Met Ser Trp Ile Gln Ser Ile Pro Phe Val His Leu Gly His Arg Asp
305 3l0 315 320
gcc ctc gag gac gac ctc ctc aac cgg aac aac tcc ttc aag ccc ttc 1008
Ala Leu Glu Asp Asp Leu Leu Asn Arg Asn Asn Ser Phe Lys Pro Phe
325 330 335
gcc gaa tac aag tcc gac tac gtc tac cag ccc ttc ccc aag acc gtc 1056
Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln Pro Phe Pro Lys Thr Val
340 345 350
tgg gag cag atc ctc aac acc tgg ctc gtc aag ccc ggc gcc ggg atc 1104
Trp Glu Gln Ile Leu Asn Thr Trp Leu Val Lys Pro Gly Ala Gly Ile
355 360 365
atg atc ttc gac ccc tac ggc gcc acc atc agc gcc acc ccg gag tcc 1152
Met Ile Phe Asp Pro Tyr Gly Ala Thr Ile Ssr Ala Thr Pro Glu Ser
370 375 380
gcc acg ccc ttc cct cac cgc aag ggc gtc ctc ttc aac atc cag tac 1200
Ala Thr Pro Phe Pro His Arg Lys Gly Val Leu Phe Asn Ile Gln Tyr
385 390 395 400
gtc aac tac tgg ttc gcc ccg gga gcc gcc gcc gcg ccc ctc tcg tgg 1248
Val Asn Tyr Trp Phe Ala Pro Gly Ala Ala Ala Ala Pro Leu Ser Trp
405 410 415
agc aag gac atc tac aac tac atg gag ccc tac gtg agc aag aac ccc 1296
Ser Lys Asp Ile Tyr Asn Tyr Met Glu Pro Tyr Val Ser Lys Asn Pro
420 425 430
agg cag gcg tac gca aac tac agg gac atc gac ctc ggc agg aac gag 1344
Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp Leu Gly Arg Asn Glu
435 440 445
gtg gtc aac gac gtc tcc acc tac gcc agc ggc aag gtc tgg ggc cag 1392
Val Val Asn Asp Val Ser Thr Tyr Ala Ser Gly Lys Val Trp Gly Gln
450 455 460
aaa tac ttc aag ggc aac ttc gag agg ctc gcc att acc aag ggc aag 1440
Lys Tyr Phe Lys Gly Asn Phe Glu Arg Leu Ala Ile Thr Lys Gly Lys
465 470 475 480
gtc gat cct acc gac tac ttc agg aac gag cag agc atc ccg ccg ctc 1488
Val Asp Pro Thr Asp Tyr Phe Arg Asn Glu Gln Ser Ile Pro Pro Leu
485 490 495
atc aaa aag tac tga 1503
Ile Lys Lys Tyr
500
<210>12
<211>500
<212>PRT
<213>梯牧草(Phleum pratense)
<400>12
Tyr Phe Pro Pro Pro Ala Ala Lys Glu Asp Phe Leu Gly Cys Leu Val
1 5 10 15
Lys Glu Ile Pro Pro Arg Leu Leu Tyr Ala Lys Ser Ser Pro Ala Tyr
20 25 30
Pro Ser Val Leu Gly Gln Thr Ile Arg Asn Ser Arg Trp Ser Ser Pro
35 40 45
Asp Asn Val Lys Pro Leu Tyr Ile Ile Thr Pro Thr Asn Val Ser His
50 55 60
Ile Gln Ser Ala Val Val Cys Gly Arg Arg His Ser Val Arg Ile Arg
65 70 75 80
Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr Arg Ser Leu
Gln Pro Glu Thr Phe Ala Val Val Asp Leu Asn Lys Met Arg Ala Val
100 105 110
Trp Val Asp Gly Lys Ala Arg Thr Ala Trp Val Asp Ser Gly Ala Gln
115 120 125
Leu Gly Glu Leu Tyr Tyr Ala Ile Tyr Lys Ala Ser Pro Thr Leu Ala
130 135 140
Phe Pro Ala Gly Val Cys Pro Thr Ile Gly Val Gly Gly Asn Phe Ala
145 150 155 160
Gly Gly Gly Phe Gly Met Leu Leu Arg Lys Tyr Gly Ile Ala Ala Glu
165 170 175
Asn Val Ile Asp Val Lys Leu Val Asp Ala Asn Gly Lys Leu His Asp
180 185 190
Lys Lys Ser Met Gly Asp Asp His Phe Trp Ala Val Arg Gly Gly Gly
195 200 205
Gly Glu Ser Phe Gly Ile Val Val Ala Trp Gln Val Lys Leu Leu Pro
210 215 220
Val Pro Pro Thr Val Thr Ile Phe Lys Ile Ser Lys Thr Val Ser Glu
225 230 235 240
Gly Ala Val Asp Ile Ile Asn Lys Trp Gln Val Val Ala Pro Gln Leu
245 250 255
Pro Ala Asp Leu Met Ile Arg Ile Ile Ala Gln Gly Pro Lys Ala Thr
260 265 270
Phe Glu Ala Met Tyr Leu Gly Thr Cys Lys Thr Leu Thr Pro Leu Met
Ser Ser Lys Phe Pro Glu Leu Gly Met Asn Pro Ser His Cys Asn Glu
290 295 300
Met Ser Trp Ile Gln Ser Ile Pro Phe Val His Leu Gly His Arg Asp
305 310 315 320
Ala Leu Glu Asp Asp Leu Leu Asn Arg Asn Asn Ser Phe Lys Pro Phe
Ala Glu Tyr Lys Ser Asp Tyr Val Tyr Gln Pro Phe Pro Lys Thr Val
340 345 350
Trp Glu Gln Ile Leu Asn Thr Trp Leu Val Lys Pro Gly Ala Gly Ile
355 360 365
Met Ile Phe Asp Pro Tyr Gly Ala Thr Ile Ser Ala Thr Pro Glu Ser
370 375 380
Ala Thr Pro Phe Pro His Arg Lys Gly Val Leu Phe Asn Ile Gln Tyr
385 390 395 400
Val Asn Tyr Trp Phe Ala Pro Gly Ala Ala Ala Ala Pro Leu Ser Trp
405 410 415
Ser Lys Asp Ile Tyr Asn Tyr Met Glu Pro Tyr Val Ser Lys Asn Pro
420 425 430
Arg Gln Ala Tyr Ala Asn Tyr Arg Asp Ile Asp Leu Gly Arg Asn Glu
435 440 445
Val Val Asn Asp Val Ser Thr Tyr Ala Ser Gly Lys Val Trp Gly Gln
450 455 460
Lys Tyr Phe Lys Gly Asn Phe Glu Arg Leu Ala Ile Thr Lys Gly Lys
465 470 475 480
Val Asp Pro Thr Asp Tyr Phe Arg Asn Glu Gln Ser Ile Pro Pro Leu
485 490 495
Ile Lys Lys Tyr
500
<210>13
<211>12
<212>PRT
<213>鸭茅(Dactylus glomerata)
<400>13
Asp Ile Tyr Asn Tyr Met Glu Pro Tyr Val Ser Lys
1 5 10
<210>14
<211>11
<212>PRT
<213>鸭茅(Dactylus glomerata)
<400>14
Val Asp Pro Thr Asp Tyr Phe Gly Asn Glu Gln
1 5 10
<210>15
<211>17
<212>PRT
<213>鸭茅(Dactylus glomerata)
<400>15
Ala Arg Thr Ala Trp Val Asp Ser Gly Ala Gln Leu Gly Glu Leu Ser
1 5 10 15
Tyr
<210>16
<211>15
<212>PRT
<213>鸭茅(Dactylus glomerata)
<400>16
Gly Val Leu Phe Asn Ile Gln Tyr Val Asn Tyr Trp Phe Ala Pro
1 5 10 15
<210>17
<211>11
<212>PRT
<213>狗牙根(Cynodon dactylon)
<400>17
Lys Thr Val Lys Pro Leu Tyr Ile Ile Thr Pro
1 5 10
<210>18
<211>22
<212>PRT
<213>狗牙根(Cynodon dactylon)
<400>18
Lys Gln Val Glu Arg Asp Phe Leu Thr Ser Leu Thr Lys Asp Ile Pro
1 5 10 15
Gln Leu Tyr Leu Lys Ser
20
<210>19
<211>16
<212>PRT
<213>狗牙根(Cynodon dactylon)
<400>19
Thr Val Lys Pro Leu Tyr Ile Ile Thr Pro Ile Thr Ala Ala Met Ile
1 5 10 15
<210>20
<211>24
<212>PRT
<213>狗牙根(Cynodon dactylon)
<400>20
Leu Arg Lys Tyr Gly Thr Ala Ala Asp Asn Val Ile Asp Ala Lys Val
1 5 10 15
Val Asp Ala Gln Gly Arg Leu Leu
20
<210>21
<211>14
<212>PRT
<213>狗牙根(Cynodon dactylon)
<400>21
Lys Trp Gln Thr Val Ala Pro Ala Leu Pro Asp Pro Asn Met
1 5 10
<210>22
<211>15
<212>PRT
<213>狗牙根(Cynodon dactylon)
<400>22
Val Thr Trp Ile Glu Ser Val Pro Tyr Ile Pro Met Gly Asp Lys
1 5 10 15
<210>23
<211>19
<212>PRT
<213>狗牙根(Cynodon dactylon)
<220>
<221>MISC_FEATURE
<222>(8)..(8)
<223>未确定氨基酸
<400>23
Gly Thr Val Arg Asp Leu Leu Xaa Arg Thr Ser Asn Ile Lys Ala Phe
1 5 10 15
Gly Lys Tyr
<210>24
<211>23
<212>PRT
<213>狗牙根(Cynodon dactylon)
<400>24
Thr Ser Asn Ile Lys Ala Phe Gly Lys Tyr Lys Ser Asp Tyr Val Leu
1 5 10 15
Glu Pro Ile Pro Lys Lys Ser
20
<210>25
<211>13
<212>PRT
<213>狗牙根(Cynodon dactylon)
<400>25
Tyr Arg Asp Leu Asp Leu Gly Val Asn Gln Val Val Gly
1 5 10
<210>26
<211>15
<212>PRT
<213>狗牙根(Cynodon dactylon)
<400>26
Ser Ala Thr Pro Pro Thr His Arg Ser Gly Val Leu Phe Asn Ile
1 5 10 15
<210>27
<211>36
<212>PRT
<213>狗牙根(Cynodon dactylon)
<400>27
Ala Ala Ala Ala Leu Pro Thr Gln Val Thr Arg Asp Ile Tyr Ala Phe
1 5 10 15
Met Thr Pro Tyr Val Ser Lys Asn Pro Arg Gln Ala Tyr Val Asn Tyr
20 25 30
Arg Asp Leu Asp
35
<210>28
<211>14
<212>PRT
<213>黑麦草(Lolinm perenne)
<400>28
Phe Leu Glu Pro Val Leu Gly Leu Ile Phe Pro Ala Gly Val
1 5 10
<210>29
<211>9
<212>PRT
<213>黑麦草(Lolium perenne)
<400>29
Gly Leu Ile Glu Phe Pro Ala Gly Val
1 5
<210>30
<211>22
<212>DNA
<213>黑麦(Secale cereale)
<400>30
ggctcccggg gcgaaccagt ag 22
<210>31
<211>23
<212>DNA
<213>黑麦(Secale cereale)
<400>31
accaacgcct cccacatcca gtc 23
<210>32
<211>49
<212>DNA
<213>黑麦(Secale cereale)
<400>32
gataagcttc tcgagtgatt agtacttttt gatcagcggc gggatgctc 49
<210>33
<211>23
<212>DNA
<213>黑麦(Secale cereale)
<400>33
gctctcgatc ggctacaatg gcg 23
<210>34
<211>25
<212>DNA
<213>黑麦(Secale cereale)
<400>34
cacgcactac aaatctccat gcaag 25
<210>35
<211>30
<212>DNA
<213>黑麦(Secale cereale)
<400>35
catgcttgat ccttattcta ctagttgggc 30
<210>36
<211>30
<212>DNA
<213>黑麦(Secale cereale)
<400>36
tacgcacgat ccttattcta ctagttgggc 30
<210>37
<211>30
<212>DNA
<213>大麦(Hordeum vulgare)
<400>37
gccttgtcct gccaccacgc cgccgccacc 30
<210>38
<211>23
<212>DNA
<213>大麦(Hordeum vulgare)
<400>38
gctctcgatc ggctacaatg gcg 23
<210>39
<211>25
<212>DNA
<213>大麦(Hordeum vulgare)
<400>39
cacgcactac aaatctccat gcaag 25
<210>40
<211>30
<212>DNA
<213>大麦(Hordeum vulgare)
<400>40
catgcttgat ccttattcta ctagttgggc 30
<210>41
<211>23
<212>DNA
<213>小麦(Triticum aestivum)
<400>41
cacgcactaa atctccatgc aag 23
<210>42
<211>30
<212>DNA
<213>小麦(Triticum aestivum)
<400>42
tacgcacgat ccttattcta ctagttgggc 30
<210>43
<211>23
<212>DNA
<213>小麦(Triticum aestivum)
<400>43
aagctctatc gcctacaatg gcg 23
<210>44
<211>25
<212>DNA
<213>小麦(Triticum aestivum)
<400>44
ggtgctcctc ttctgcgcct tgtcc 25
Claims (18)
1.一种DNA分子，其与选自SEQ ID NO 1、3、5、7和9的序列之一的谷类花粉主要致敏原的核苷酸序列相对应。
2.一种DNA分子，其在严格条件下与权利要求1中的DNA分子杂交并来源于禾本科物种的DNA序列。
3.一种编码多肽的DNA分子，其与来自黑麦(Secale cerale)、大麦(Hordeum vulgare)或小麦(Triticum aestivum)的主要致敏原Sec c4、Hor v4或Tri a4有免疫交叉反应并来源于禾本科物种的DNA序列。
4.一种DNA分子，其与权利要求1到3中的一项或多项部分序列或部分序列的组合相对应并且编码禾本科的第4组致敏原的免疫调节、T细胞反应片段。
5.一种与权利要求1到4中的一项或多项编码免疫调节性T细胞反应片段的核苷酸序列相对应的DNA分子，其特征是通过对单个密码子的特异性突变、删除或添加而特异性修饰所述核苷酸序列。
6.权利要求5中的DNA分子，其特征是所述突变导致了相应多肽的一个、多个或所有半胱氨酸被其他氨基酸替代。
7.一种重组DNA表达载体或克隆系统，其包含功能性连接到表达控制序列的权利要求1到6中一项或多项的DNA分子。
8.一种宿主生物，其用权利要求1到6中一项或多项的DNA分子或权利要求7中的表达载体转化。
9.一种制备由权利要求1到6中一项或多项的DNA序列编码的多肽的方法，所述方法为对权利要求8中的宿主生物进行培养并从培养物中分离相应的多肽。
10.一种与SEQ ID NO 2、4、6、8和10中氨基酸序列之一对应的多肽，其由权利要求1到6中一项或多项的DNA序列编码。
11.一种与权利要求10中的氨基酸序列的成熟致敏原相应的多肽，选自以下氨基酸序列：
-一段SEQ ID NO 2、4或6的氨基酸序列，起始于第23位氨基酸；
-一段SEQ ID NO 8或10的氨基酸序列，起始于第22位氨基酸。
12.权利要求10或11中的多肽，其用作药物。
13.一种包含至少一种权利要求12中的多肽并任选包含其他活性物质和/或佐剂的药用组合物，其用于诊断和/或治疗来自禾本科的第4组致敏原参与触发的过敏反应。
14.至少一种权利要求12中的多肽在制备用于诊断和/或治疗来自禾本科的第4组致敏原参与触发的过敏反应的药物中的用途。
15.权利要求1到6中一项或多项的DNA分子，其用作药物。
16.权利要求7中的重组表达载体，其用作药物。
17.一种包含至少一种权利要求15中的DNA分子或至少一种权利要求16中的表达载体并任选包含其他活性成分和/或佐剂的药用组合物，其用于免疫治疗性DNA疫苗接种来自禾本科的第4组致敏原参与触发的过敏反应病人和/或预防这样的过敏反应。
18.至少一种权利要求15中的DNA分子或至少一种权利要求16中的表达载体在制备用于免疫治疗性DNA疫苗接种来自禾本科的第4组致敏原参与触发的过敏反应病人和/或预防这样的过敏反应的药物中的用途。
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10359351A DE10359351A1 (de) | 2003-12-16 | 2003-12-16 | DNA-Sequenz und rekombinante Herstellung von Gruppe-4 Majorallergenen aus Getreiden |
| DE10359351.9 | 2003-12-16 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN2013102182194A Division CN103305531A (zh) | 2003-12-16 | 2004-12-01 | 谷类第4组主要致敏原的dna序列和重组制品 |
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| CN1894410A true CN1894410A (zh) | 2007-01-10 |
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| CN2013102182194A Pending CN103305531A (zh) | 2003-12-16 | 2004-12-01 | 谷类第4组主要致敏原的dna序列和重组制品 |
| CNA2004800372314A Pending CN1894410A (zh) | 2003-12-16 | 2004-12-01 | 谷类第4组主要致敏原的dna序列和重组制品 |
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| CN2013102182194A Pending CN103305531A (zh) | 2003-12-16 | 2004-12-01 | 谷类第4组主要致敏原的dna序列和重组制品 |
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| Country | Link |
|---|---|
| US (3) | US7935347B2 (zh) |
| EP (3) | EP1709177B1 (zh) |
| JP (1) | JP4960102B2 (zh) |
| CN (2) | CN103305531A (zh) |
| AT (1) | ATE501257T1 (zh) |
| AU (1) | AU2004299600A1 (zh) |
| BR (1) | BRPI0417736A (zh) |
| CA (3) | CA2873261C (zh) |
| DE (2) | DE10359351A1 (zh) |
| ES (3) | ES2443302T3 (zh) |
| PL (3) | PL2261336T3 (zh) |
| PT (3) | PT2261337E (zh) |
| RU (1) | RU2373283C2 (zh) |
| WO (1) | WO2005059136A2 (zh) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10359351A1 (de) * | 2003-12-16 | 2005-07-21 | Merck Patent Gmbh | DNA-Sequenz und rekombinante Herstellung von Gruppe-4 Majorallergenen aus Getreiden |
| EP3125933B1 (en) | 2014-03-31 | 2022-05-18 | Boehringer Ingelheim Vetmedica GmbH | Improved modular antigen transportation molecules and uses therof |
| CA2999930A1 (en) | 2015-09-30 | 2017-04-06 | Boehringer Ingelheim Vetmedica Gmbh | Improved modular antigen transportation molecules and uses therof in animals |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5762943A (en) * | 1996-05-14 | 1998-06-09 | Ribi Immunochem Research, Inc. | Methods of treating type I hypersensitivity using monophosphoryl lipid A |
| US7214786B2 (en) * | 2000-12-14 | 2007-05-08 | Kovalic David K | Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement |
| WO2003000898A1 (en) * | 2001-06-22 | 2003-01-03 | Syngenta Participations Ag | Plant genes involved in defense against pathogens |
| AUPR779201A0 (en) | 2001-09-20 | 2001-10-11 | University Of Melbourne, The | Immunotherapeutic and immunoprophylactic reagents |
| AU2002328225A1 (en) | 2001-09-21 | 2003-04-01 | Affinium Pharmaceuticals, Inc. | Bacterial polypeptides involved in protein processing |
| WO2004000881A1 (de) * | 2002-06-25 | 2003-12-31 | Merck Patent Gmbh | Dna-sequenz und rekombinante herstellung des graspollen-allergens phl p4 |
| DE10359351A1 (de) * | 2003-12-16 | 2005-07-21 | Merck Patent Gmbh | DNA-Sequenz und rekombinante Herstellung von Gruppe-4 Majorallergenen aus Getreiden |
-
2003
- 2003-12-16 DE DE10359351A patent/DE10359351A1/de not_active Withdrawn
-
2004
- 2004-12-01 PT PT100096783T patent/PT2261337E/pt unknown
- 2004-12-01 RU RU2006125511/13A patent/RU2373283C2/ru not_active IP Right Cessation
- 2004-12-01 BR BRPI0417736-3A patent/BRPI0417736A/pt not_active Application Discontinuation
- 2004-12-01 PL PL10009677T patent/PL2261336T3/pl unknown
- 2004-12-01 AT AT04803421T patent/ATE501257T1/de active
- 2004-12-01 PT PT100096775T patent/PT2261336E/pt unknown
- 2004-12-01 AU AU2004299600A patent/AU2004299600A1/en not_active Abandoned
- 2004-12-01 CN CN2013102182194A patent/CN103305531A/zh active Pending
- 2004-12-01 DE DE502004012294T patent/DE502004012294D1/de active Active
- 2004-12-01 JP JP2006544263A patent/JP4960102B2/ja not_active Expired - Fee Related
- 2004-12-01 ES ES10009678.3T patent/ES2443302T3/es active Active
- 2004-12-01 ES ES04803421T patent/ES2363015T3/es active Active
- 2004-12-01 CN CNA2004800372314A patent/CN1894410A/zh active Pending
- 2004-12-01 WO PCT/EP2004/013664 patent/WO2005059136A2/de active Application Filing
- 2004-12-01 CA CA2873261A patent/CA2873261C/en not_active Expired - Fee Related
- 2004-12-01 EP EP04803421A patent/EP1709177B1/de not_active Not-in-force
- 2004-12-01 PL PL04803421T patent/PL1709177T3/pl unknown
- 2004-12-01 EP EP10009677.5A patent/EP2261336B1/de not_active Not-in-force
- 2004-12-01 EP EP10009678.3A patent/EP2261337B1/de not_active Not-in-force
- 2004-12-01 CA CA2945267A patent/CA2945267A1/en not_active Abandoned
- 2004-12-01 ES ES10009677.5T patent/ES2442951T3/es active Active
- 2004-12-01 PL PL10009678T patent/PL2261337T3/pl unknown
- 2004-12-01 PT PT04803421T patent/PT1709177E/pt unknown
- 2004-12-01 US US10/583,089 patent/US7935347B2/en not_active Expired - Fee Related
- 2004-12-01 CA CA2549573A patent/CA2549573C/en not_active Expired - Fee Related
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2010
- 2010-11-16 US US12/947,361 patent/US9309297B2/en not_active Expired - Fee Related
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2015
- 2015-12-17 US US14/972,633 patent/US20170246317A1/en not_active Abandoned
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