CN1861017A - Heat-shock and static water-pressure type method for prodn. of tetraploid of trout - Google Patents
Heat-shock and static water-pressure type method for prodn. of tetraploid of trout Download PDFInfo
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- CN1861017A CN1861017A CN 200510114672 CN200510114672A CN1861017A CN 1861017 A CN1861017 A CN 1861017A CN 200510114672 CN200510114672 CN 200510114672 CN 200510114672 A CN200510114672 A CN 200510114672A CN 1861017 A CN1861017 A CN 1861017A
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Abstract
A method for inducing the tetraploid salmoniform rainbow trout by hot shock or static water pressure process features that the fertilized ova of rainbow trout is treated by hot shock at 30 deg.C for 5-6 min or static water pressure under 600-650 kg/sq.cm for 5-6 min.
Description
Technical field
The present invention can produce fresh water cold water fishes tetraploid colony, comprises rainbow trout, golden trout, Atlantic salmon and brook trout.Tetraploid parent fish and normal diploid hybridization can obtain 100% triploid fish.
Background technology
The fresh water cold water fishes triploid of culturing is to induce by chemical method, heat shock or hydrostatic pressing to form, but all can not obtains 100% triploid fry at present, and need evaluation and screening, workload is big, and accuracy rate is not high, and the fry survival rate is low in the breeding process.
Summary of the invention
In order to overcome the low problem of the not pure and mild survival rate of the germplasm that occurs in the triploid fry breeding process in the actual production, the invention provides a kind of new fish breeding approach.Adopt the method for heat shock and hydrostatic pressing all can obtain Fish tetraploid colony, 100% triploid is produced in this colony and common diploid hybridization in enormous quantities, makes production triploid fry technology become a kind of simple hybridization technique.
The technical scheme that its technical problem that solves this utility model adopts is: utilize hydrostatic pressing generator or heat shock plant equipment, the liploid fish germ cell that the spermatozoa insemination method obtained is induced processing, stop germ cell in the developmental spilting of an egg first time, thereby induced chromosome multiplication in the nucleus, produced tetraploid fish.Through repeated experiments repeatedly, and then obtain tetraploid colony.
The invention has the beneficial effects as follows, can simplify the production technology of triploid fish, make doubly change rate of triploid reach 100%.
Description of drawings
Fig. 1 is the heat production device construction figure of heat shock among the present invention.
Fig. 2 is the regulating tank structural map of heat shock among the present invention
Fig. 3 is the water-bath device construction figure of heat shock among the present invention.
Fig. 4 is the generator structural map of hydrostatic pressing among the present invention.
(1) adds fuel inlet among the figure, (2) thermometer, delivery line after (3) water heats up, (4) hot water inlet water tank conduit, (5) cold water inlet water tank conduit, (6) thermometer, (7) regulate the water delivery line, (8) water tank, (9) place side by side the formula incubation tank, (10) hot-water storage pond, (11) water yield is regulated halter, (12) hydrostatic pressing generator, (13) hydrostatic pressing conducting tube, (14) hydrostatic pressing is to the germ cell acting device.
The specific embodiment
In Fig. 1,2,3, fuel enters from adding fuel inlet (1), the burning production capacity heats up water, to temperature about 40 ℃ (2), high-temperature water is derived by delivery line (3), imports water tank (8) by hot water inlet water tank conduit (4), behind 15 ℃ of cold water of mixing by cold water inlet water tank conduit (5) importing, water temperature is adjusted to 30 ℃ (6), regulates halter (8) through the water yield and enters hot water regulating reservoir (11) by regulating water delivery line (7), and germ cell putting into placed side by side formula incubation tank (9) processing of suffering a shock.
In Fig. 4, to pack into plastic tube and to put into hydrostatic pressing to germ cell acting device (14) after the germ cell, hydrostatic pressing generator (12) produces hydrostatic pressing, is changed over to the germ cell acting device by hydrostatic pressing conducting tube (13), to the germ cell processing of suffering a shock.
Claims (1)
1. heat shock and hydrostatic pressing method are induced tetraploid salmon Squaliobarbus ourriculus, it is characterized in that handling with heat shock or hydrostatic platen press behind the fish roe artificial fertilization, and the heat shock zero-time is insemination back 5h, and water temperature is 30 ℃, continues 5～6min; The hydrostatic pressing zero-time is insemination back 5h, and pressure is 600～650Kg/cm
2, the persistent period is 5～6min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510114672 CN1861017A (en) | 2005-10-28 | 2005-10-28 | Heat-shock and static water-pressure type method for prodn. of tetraploid of trout |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510114672 CN1861017A (en) | 2005-10-28 | 2005-10-28 | Heat-shock and static water-pressure type method for prodn. of tetraploid of trout |
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| Publication Number | Publication Date |
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| CN1861017A true CN1861017A (en) | 2006-11-15 |
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| CN 200510114672 Pending CN1861017A (en) | 2005-10-28 | 2005-10-28 | Heat-shock and static water-pressure type method for prodn. of tetraploid of trout |
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| CN (1) | CN1861017A (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101595849B (en) * | 2009-06-23 | 2011-02-23 | 中国水产科学研究院黄海水产研究所 | Flounder tetraplont fry batch induction method |
| CN102090360A (en) * | 2010-12-17 | 2011-06-15 | 中国水产科学研究院黄海水产研究所 | High-efficient induction method of tetraploid cynoglossus semilaevis fish fries |
| CN102106292A (en) * | 2011-02-10 | 2011-06-29 | 中国水产科学研究院黄海水产研究所 | Flounder triploid batch producing method |
| CN102696515A (en) * | 2012-06-01 | 2012-10-03 | 北京市水产科学研究所 | Preparation method of game fish triploid fries |
| CN103109764A (en) * | 2013-01-30 | 2013-05-22 | 中国水产科学研究院黄海水产研究所 | Automatic pressurization hydrostatic pressure type fish chromosome group double operating device |
| CN104109652A (en) * | 2014-07-04 | 2014-10-22 | 湖南师范大学 | Artificially induced tetraploid crucian cell line and culture method and application thereof |
| CN105941330A (en) * | 2016-06-30 | 2016-09-21 | 湖南文理学院 | Efficient breeding method for male tetraploid pure lines of soft-shelled turtles |
| CN106035234A (en) * | 2016-06-30 | 2016-10-26 | 湖南文理学院 | Efficient breeding method for male tetraploid pure line of tortoises |
| CN106106278A (en) * | 2016-06-30 | 2016-11-16 | 湖南文理学院 | Corbicula fluminea triploid is sheerly efficient breeding method |
| CN106135125A (en) * | 2016-06-30 | 2016-11-23 | 湖南文理学院 | Frog triploid is sheerly efficient breeding method |
| CN109362616A (en) * | 2018-12-17 | 2019-02-22 | 中国水产科学研究院黑龙江水产研究所 | A kind of breeding method of triploid carp |
| CN109496922A (en) * | 2018-11-14 | 2019-03-22 | 中国海洋大学 | A kind of producing method for seed of hydrostatic pressing induction rainbow trout tetraploid |
-
2005
- 2005-10-28 CN CN 200510114672 patent/CN1861017A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101595849B (en) * | 2009-06-23 | 2011-02-23 | 中国水产科学研究院黄海水产研究所 | Flounder tetraplont fry batch induction method |
| CN102090360A (en) * | 2010-12-17 | 2011-06-15 | 中国水产科学研究院黄海水产研究所 | High-efficient induction method of tetraploid cynoglossus semilaevis fish fries |
| CN102090360B (en) * | 2010-12-17 | 2012-02-29 | 中国水产科学研究院黄海水产研究所 | High-efficient induction method of tetraploid cynoglossus semilaevis fish fries |
| CN102106292A (en) * | 2011-02-10 | 2011-06-29 | 中国水产科学研究院黄海水产研究所 | Flounder triploid batch producing method |
| CN102696515A (en) * | 2012-06-01 | 2012-10-03 | 北京市水产科学研究所 | Preparation method of game fish triploid fries |
| CN103109764A (en) * | 2013-01-30 | 2013-05-22 | 中国水产科学研究院黄海水产研究所 | Automatic pressurization hydrostatic pressure type fish chromosome group double operating device |
| CN104109652A (en) * | 2014-07-04 | 2014-10-22 | 湖南师范大学 | Artificially induced tetraploid crucian cell line and culture method and application thereof |
| CN105941330A (en) * | 2016-06-30 | 2016-09-21 | 湖南文理学院 | Efficient breeding method for male tetraploid pure lines of soft-shelled turtles |
| CN106035234A (en) * | 2016-06-30 | 2016-10-26 | 湖南文理学院 | Efficient breeding method for male tetraploid pure line of tortoises |
| CN106106278A (en) * | 2016-06-30 | 2016-11-16 | 湖南文理学院 | Corbicula fluminea triploid is sheerly efficient breeding method |
| CN106135125A (en) * | 2016-06-30 | 2016-11-23 | 湖南文理学院 | Frog triploid is sheerly efficient breeding method |
| CN109496922A (en) * | 2018-11-14 | 2019-03-22 | 中国海洋大学 | A kind of producing method for seed of hydrostatic pressing induction rainbow trout tetraploid |
| CN109362616A (en) * | 2018-12-17 | 2019-02-22 | 中国水产科学研究院黑龙江水产研究所 | A kind of breeding method of triploid carp |
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Open date: 20061115 |