CN1748493A - Cultivation technology and the feed formula of aseptic cavy - Google Patents
Cultivation technology and the feed formula of aseptic cavy Download PDFInfo
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- CN1748493A CN1748493A CNA2005100865835A CN200510086583A CN1748493A CN 1748493 A CN1748493 A CN 1748493A CN A2005100865835 A CNA2005100865835 A CN A2005100865835A CN 200510086583 A CN200510086583 A CN 200510086583A CN 1748493 A CN1748493 A CN 1748493A
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- cavy
- aseptic
- cobastab
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Abstract
The invention discloses cultivation technology and the feed formula of a kind of aseptic cavy.The inventor cuts open the belly and takes out fetus in the sterile barrier system, and raise and breed in aseptic isolator, according to the cavy dietary structure, be that raw material is made feed, warp with protein, carbohydrate, fat, vitamin and mineral matter
60The cobalt irradiation sterilization, the composition of feed, form and smell are fit to habit and the hobby of cavy, and good palatability is balanced in nutrition comprehensive, aseptic cavy survival rate height with this forage feed, effective, there are not microorganism alive and parasite or worm's ovum to infect, make experimental result correctly reliable, the feed making process is simple, price is cheaper, is convenient to promote, and can be widely used in the research and the production of aspects such as pharmacology, pharmacology, epiphytology, immunology, alimentology.
Description
Technical field
The invention discloses cultivation technology and the feed formula of a kind of aseptic cavy, belong to field of biology.
Background technology
Germfree animal just is meant the microorganism and the parasitic animal that can not detect any work.From microbiological viewpoint, usually in the body of laboratory animal and externally have a parasite, also often have bacterium and virus in the body, be difficult to get rid of some potential infectious disease.In addition, contain antibody in the serum of common laboratory animal.So carry out medical scientific with conventional animal, can there be various interference, experimental result is often imprecise.Use germfree animal to do experiment and just can overcome the existing shortcoming of common experiment, make experimental result correctly reliable.
Cavy is because characteristics such as its altricialism, sex premature, allergy sensitivity are widely used in the research and the production of aspects such as pharmacology, pharmacology, epiphytology, immunology, alimentology.The acquisition technology of aseptic cavy is very ripe, but aseptic cavy feed fails thoroughly to solve all the time, has become the bottleneck problem of aseptic cavy development.Baeot and Harden find that aseptic fly-maggot must be fed vitamin B group could normal growth, the needs of germfree animal have been proved to vitamin B group, because there is not bacterium to exist in the germfree animal intestines, self can not synthesize vitamin B group, thereby has found the reason of raising the germfree animal failure in the past.
The feed of aseptic cavy, must be through sterilization fully, the sterilizing methods of aseptic cavy feed, multipurpose high-pressure sterilization at present, or radiation sterilization, or the sterilization of ethylene oxide sterilizing method, nutrition is destroyed significantly, the feed holding time is short, must replenish the nutrition of destroying because of autoclaving, as: vitamin B1, vitamin C, pantothenic acid etc.
Summary of the invention
Purpose of the present invention is exactly the problem that exists at above-mentioned prior art, and a kind of safety economy that provides, balanced in nutrition sterilization is abundant comprehensively, the aseptic cavy feed of good palatability.
Good effect of the present invention:
1, this feed carries out prescription according to the cavy dietary structure, and is balanced in nutrition comprehensive, the good palatability of feed;
2, with the survival rate height of the aseptic cavy of this forage feed, effective;
3, with the aseptic cavy of this forage feed, there are not microorganism alive and parasite or worm's ovum to infect, make experimental result correctly reliable;
4, the composition of feed, form and smell are fit to habit and the hobby of cavy;
5, the feed making process is simple, and price is cheaper, is convenient to promote.
Aseptic cavy is in the sterile barrier system, cuts open the belly and takes out fetus, raises and breeds in aseptic isolator, and aseptic cavy outward appearance is identical with conventional animal, but its function, structure and conventional animal are very different.
Because there is not bacterium to exist in the germfree animal intestines, self can not synthesize vitamin B group, one of reason of the germfree animal failure that past raises, be exactly not have replenishing vitamins in the feed, this feed addresses this problem satisfactorily, this feed formula is tested in a large number and is drawn, and each amounts of components all has better curative effect in the following weight parts scope.
Ten thousand parts of ten thousand parts of protein 1-1.5, carbohydrate 5-6, ten thousand parts of fatty 2.5-3, retinol1 5-25 million international units, cholecalciferol-5 million international units, vitamin E 0.8-1.1 million international units, vitamin K1 0.05-0.07 part, Cobastab
10.6-1 part, Cobastab
21-1.5 part, Cobastab
60.4-0.6 part, Cobastab
120.001-0.002 part, vitamin C 50-100 part, nicotinic acid 3-5 part, folic acid 0.06-0.07 part, pantothenic acid 2-3 part, calcium 300-500 part, phosphorus 200-400 part, magnesium 40-60 part, iron 5-10 part, zinc 4-6 part, manganese 0.05-0.12 part, copper 0.3-0.6 part, iodine 0.5-1.5 part, sodium 10-150 part, potassium 500-700 part, chlorine 300-500 part.
Be preferably: 12000 parts in protein, 56000 parts in carbohydrate, 28000 parts in fat, dehydroretinol 000000 international unit, VD4 00000 international unit, vitamin e1 0000 international unit, vitamin K
10.06 part, Cobastab
10.8 part, Cobastab
21.2 part, Cobastab
60.5 part, Cobastab
120.001.6 part, 75 parts of vitamin Cs, 4 parts in nicotinic acid, 0.065 part in folic acid, 2.5 parts in pantothenic acid, 400 parts of calcium, 300 parts in phosphorus, 50 parts in magnesium, 7 parts of iron, 5 parts on zinc, 0.1 part in manganese, 0.5 part of copper, 0.1 part of iodine, 125 parts in sodium, 600 parts in potassium, 400 parts of chlorine.
Another aspect of the present invention relates to a kind of sterilizing methods of aseptic cavy feed.
Feed is packed with plastic bag sealing, through 20-30KGY dosage
60The cobalt irradiation sterilization is imported aseptic isolator into through the sterilization aqueduct again, and the sterile water of dissolving feed is through high pressure 118-124 degree centigrade and sterilization in 40-50 minute.
With
60Cobalt irradiation feed sterilization effect is remarkable, and the holding time is long, can keep germ-free condition to reach more than 6 months, nutrition such as Cobastab
1, Cobastab
2, destruction higher pressure sterilization such as vitamin C, protein, cystine, lysine, pantothenic acid significantly reduces.
Aseptic detection is cultivated young cavy with the artificial suckling method ripe, at 30 ~ 35 days during from breast, detects fresh excreta and throat swab with sodium thioglycollate medium (TGC) and brain heart body fluid medium (TSA), 25 ℃ and 37 ℃ of cultivations 17-19 days.
First aseptic cavy of microbial inoculant is behind breast, and inoculate 5 kinds of microorganisms: aseptic cavy does not have the attack of microorganism, passive protective physical fitness a little less than, therefore inoculating lactobacillus at first, Bifidobacterium inoculates enterococcus after one week, enterobacteria and bacteroid, route of inoculation are oral, and inoculum concentration is 10
6Determine to be colonizated in the cavy body after checking, not having specially appointed microorganism, promptly to look this animal be germfree animal.
Fresh excreta, the cavy sample (comprising the institute adult cavy in generation in postpartum) of living of aseptic cavy after microorganism detection is sterilized with this sterilizing methods, send Nat'l Pharmaceutical ﹠ Biological Products Control Institute Experimental Animal Center laboratory animal standardization chamber by GB 1 492_94 and the every monthly test micrometer of GB/T 149261_41_94 biological aspect, comprise: parasite, bacterium, virus, aseptic cavy assay meets the classification standard of aseptic cavy.
The present invention will further specify by embodiment:
Embodiment
Embodiment one
Take by weighing each raw material according to the following weight proportioning:
10,000 parts in protein, 50,000 parts in carbohydrate, 2.5 ten thousand parts in fat, retinol1 5 million international units, cholecalciferol million international units, vitamin E 0.8 million international units, vitamin K
10.05 part, Cobastab
10.6 part, Cobastab
21 part, Cobastab
60.4 part, Cobastab
120.001 part, 50 parts of vitamin Cs, 3 parts in nicotinic acid, 0.06 part in folic acid, 2 parts in pantothenic acid, 300 parts of calcium, 200 parts in phosphorus, 40 parts in magnesium, 5 parts of iron, 4 parts on zinc, 0.05 part in manganese, 0.3 part of copper, 0.5 part of iodine, 10 parts in sodium, 500 parts in potassium, 300 parts of chlorine.
With protein, carbohydrate, fat, vitamin A, vitamin D, vitamin E, vitamin K
1, Cobastab
1, Cobastab
2, Cobastab
6, Cobastab
12, vitamin C, nicotinic acid, folic acid, pantothenic acid, calcium, phosphorus, magnesium, iron, zinc, manganese, copper, iodine, sodium, potassium, chlorine pack with plastic bag sealing, through 20-30KGY dosage
60The cobalt irradiation sterilization is imported aseptic isolator into through the sterilization aqueduct again; The sterile water of dissolving feed is deposited in aseptic after the sterilization of high pressure 118-124 degree centigrade and 40-50 minute, during nursing again through high pressure 118-124 degree centigrade and sterilization in 40-50 minute.
The artificial suckling left hand is held the newborn animal Head and Neck, the right hand is held nipple and is inserted in the newborn animal mouth, push artificial nipple gently, make and flow out a milk, induced animal is sucked, during lactation, notice will be concentrated, and notes the breast in the animal mouth constantly, if the breast in the animal mouth too much, should control flow velocity, prevent that animal from choking.
Nursing time a day every day is 8:00 early, afternoon 2:00, regularly lactation of late 8:00.
The estimation of value for feeding animal value for feeding is calculated one day lactation total amount by 20% of body weight, is each value for feeding after 3, and determines concrete value for feeding according to circumstances.
Embodiment two
Take by weighing each component raw material according to the following weight proportioning:
1.5 ten thousand parts in protein, 60,000 parts in carbohydrate, 30,000 parts in fat, dehydroretinol 5 million international units, Vitamin D5 million international units, vitamin e1 .1 million international units, vitamin K
10.07 part, Cobastab
11 part, Cobastab
21.5 part, Cobastab
60.6 part, Cobastab
120.002 part, 100 parts of vitamin Cs, 5 parts in nicotinic acid, 0.07 part in folic acid, 3 parts in pantothenic acid, 500 parts of calcium, 400 parts in phosphorus, 60 parts in magnesium, 10 parts of iron, 6 parts on zinc, 0.12 part in manganese, 0.6 part of copper, 1.5 parts of iodine, 150 parts in sodium, 700 parts in potassium, 500 parts of chlorine.
The artificial suckling left hand is held the newborn animal Head and Neck, and the right hand is held nipple and inserted in the newborn animal mouth, pushes artificial nipple gently, makes to flow out a milk, and induced animal is sucked.
Nursing time a day every day is 8:00 early, afternoon 2:00, regularly lactation of late 8:00.
Value for feeding is with embodiment one.
Embodiment three
Take by weighing each component raw material according to the following weight proportioning:
12000 parts in protein, 56000 parts in carbohydrate, 28000 parts in fat, dehydroretinol 000000 international unit, VD4 00000 international unit, vitamin e1 0000 international unit, vitamin K
10.06 part, Cobastab
10.8 part, Cobastab
21.2 part, Cobastab
60.5 part, Cobastab
120.001.6 part, 75 parts of vitamin Cs, 4 parts in nicotinic acid, 0.065 part in folic acid, 2.5 parts in pantothenic acid, 400 parts of calcium, 300 parts in phosphorus, 50 parts in magnesium, 7 parts of iron, 5 parts on zinc, 0.1 part in manganese, 0.5 part of copper, 0.1 part of iodine, 125 parts in sodium, 600 parts in potassium, 400 parts of chlorine.
Lactation method, nursing time a day, value for feeding are with embodiment one.
Claims (5)
1. method that detects aseptic cavy, it is characterized in that: young cavy is cultivated ripe with the artificial suckling method, at 30 ~ 35 days during, detect fresh excreta and throat swab with sodium thioglycollate medium and brain heart body fluid medium, 25 ℃ and 37 ℃ of cultivations 17-19 days from breast.
2. the feed formula of an aseptic cavy is characterized in that this prescription made by following materials of weight proportions: ten thousand parts of ten thousand parts of protein 1-1.5, carbohydrate 5-6, ten thousand parts of fatty 2.5-3, retinol1 5-25 million international units, cholecalciferol-5 million international units, vitamin E 0.8-1.1 million international units, vitamin K
10.05-0.07 part, Cobastab
10.6-1 part, Cobastab
21-1.5 part, Cobastab
60.4-0.6 part, Cobastab
120.001-0.002 part, vitamin C 50-100 part, nicotinic acid 3-5 part, folic acid 0.06-0.07 part, pantothenic acid 2-3 part, calcium 300-500 part, phosphorus 200-400 part, magnesium 40-60 part, iron 5-10 part, zinc 4-6 part, manganese 0.05-0.12 part, copper 0.3-0.6 part, iodine 0.5-1.5 part, sodium 10-150 part, potassium 500-700 part, chlorine 300-500 part.
3. a kind of aseptic cavy feed formula according to claim 2 is characterized in that the preferable amount of each raw material is: 1.2 ten thousand parts in protein, 5.6 ten thousand parts in carbohydrate, 2.8 ten thousand parts in fat, dehydroretinol 0 million international units, VD4 million international units, vitamin e1 million international units, vitamin K
10.06 part, Cobastab
10.8 part, Cobastab
21.2 part, Cobastab
60.5 part, Cobastab
120.0016 part, 75 parts of vitamin Cs, 4 parts in nicotinic acid, 0.065 part in folic acid, 2.5 parts in pantothenic acid, 400 parts of calcium, 300 parts in phosphorus, 50 parts in magnesium, 7 parts of iron, 5 parts on zinc, 0.1 part in manganese, 0.5 part of copper, 0.1 part of iodine, 125 parts in sodium, 600 parts in potassium, 400 parts of chlorine.
4. the sterilizing methods of an aseptic cavy feed formula, it is characterized in that: feed is through 20-30KGY dosage
60The cobalt irradiation sterilization.
5. the sterilizing methods of a kind of aseptic cavy feed formula according to claim 4 is characterized in that: the sterile water of dissolving feed, and through high pressure 118-124 degree centigrade and sterilization in 40-50 minute.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2005100865835A CN1748493A (en) | 2005-10-11 | 2005-10-11 | Cultivation technology and the feed formula of aseptic cavy |
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| CNA2005100865835A CN1748493A (en) | 2005-10-11 | 2005-10-11 | Cultivation technology and the feed formula of aseptic cavy |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104996359A (en) * | 2015-07-29 | 2015-10-28 | 北海市铁山港区富群养殖有限公司 | Baby black Guinea pig breeding method |
| CN105028951A (en) * | 2015-08-05 | 2015-11-11 | 山西省肿瘤研究所 | Vitamin additive for cleaning-grade guinea pig raising and breeding |
| CN106342749A (en) * | 2016-08-24 | 2017-01-25 | 广西河池市明达特种养殖专业合作社 | Guinea pig breeding method |
| CN106359258A (en) * | 2016-08-25 | 2017-02-01 | 广西河池市明达特种养殖专业合作社 | Disease-resisting breeding method for guinea pig |
| CN106376519A (en) * | 2016-08-25 | 2017-02-08 | 广西河池市明达特种养殖专业合作社 | Guinea pig breeding method |
| CN108576454A (en) * | 2018-03-06 | 2018-09-28 | 睿迪生物科技（深圳）有限公司 | A kind of cavy milk powder and the preparation method and application thereof |
| CN108925502A (en) * | 2018-05-30 | 2018-12-04 | 凌云县郁丰农业专业合作社联合社 | A kind of mating system of Huang globefish |
| CN109123102A (en) * | 2018-09-27 | 2019-01-04 | 江苏省协同医药生物工程有限责任公司 | A kind of aseptic experiment mouse feed and preparation method thereof |
| CN111685238A (en) * | 2020-07-15 | 2020-09-22 | 常州鼠一鼠二生物科技有限公司 | Sterile feed for large sterile mice and preparation method thereof |
-
2005
- 2005-10-11 CN CNA2005100865835A patent/CN1748493A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104996359A (en) * | 2015-07-29 | 2015-10-28 | 北海市铁山港区富群养殖有限公司 | Baby black Guinea pig breeding method |
| CN105028951A (en) * | 2015-08-05 | 2015-11-11 | 山西省肿瘤研究所 | Vitamin additive for cleaning-grade guinea pig raising and breeding |
| CN106342749A (en) * | 2016-08-24 | 2017-01-25 | 广西河池市明达特种养殖专业合作社 | Guinea pig breeding method |
| CN106359258A (en) * | 2016-08-25 | 2017-02-01 | 广西河池市明达特种养殖专业合作社 | Disease-resisting breeding method for guinea pig |
| CN106376519A (en) * | 2016-08-25 | 2017-02-08 | 广西河池市明达特种养殖专业合作社 | Guinea pig breeding method |
| CN108576454A (en) * | 2018-03-06 | 2018-09-28 | 睿迪生物科技（深圳）有限公司 | A kind of cavy milk powder and the preparation method and application thereof |
| CN108925502A (en) * | 2018-05-30 | 2018-12-04 | 凌云县郁丰农业专业合作社联合社 | A kind of mating system of Huang globefish |
| CN109123102A (en) * | 2018-09-27 | 2019-01-04 | 江苏省协同医药生物工程有限责任公司 | A kind of aseptic experiment mouse feed and preparation method thereof |
| CN111685238A (en) * | 2020-07-15 | 2020-09-22 | 常州鼠一鼠二生物科技有限公司 | Sterile feed for large sterile mice and preparation method thereof |
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Open date: 20060322 |